Thermo Scientific Proteomics & Metabolomics Seminar Tour

Transcription

1 Proteomics & Seminar Tour Ignite your workflows with our with Cutting Edge LC-MS Technology Stop by to learn the latest advances in mass spectrometry workflows, technologies launched at 2012 ASMS and talk with our application experts and local guest speakers at our interactive seminar tour. Topics for discussion include: An insight into qualitative and quantitative proteomics* Cutting edge metabolomics and lipidomics research utilizing HRAM and the Q Exactive. HRAM quantification for verification, Data Independent Acquisition (DIA) methods, and more!, workflow including acquisition methods, enrichment, consumables and Byonics. Guest experts from local customer users at select locations. Q Exactive MS TSQ Vantage Orbitrap Elite Register for a location near you: Tues, Jan 15, 2013 Ithaca, NY at Cornell University Wed, Jan 16, 2013 Baltimore, MD at John Hopkins University Tues, Nov 6, 2012 Kansas City, KS at University of Kansas Medical Center Wed, Nov 7, 2012 St. Louis, MO at Washington University Thrs, Nov 8, 2012 Madison, WI at UW Biotech Center Frid, Nov 9, 2012 Cleveland, OH at Case Western Reserve University Tues, Nov 13, 2012 Louisville, KY at University of Louisville Health Sciences Campus Wed, Nov 14, 2012 Bloomington, IN at Indiana University Thrs, Nov 15, 2012 Chicago, IL at University of Chicago Frid, Nov 16, 2012 Rochester, MN at Mayo Clinic Reserve your seat at Space is limited. Free to attend

2 Hot application : Tandem mass tags: New strategies for higher multiplexing and for cysteine peptide eodification, enrichment, and multiplexed quantitation Tandem Mass Tag (TMT) Reagents enable concurrent identification and multiplexed quantitation of proteins in different samples using tandem mass spectrometry. We will describe an irreversible, cysteine-reactive TMT reagent set containing an iodoacetyl reactive group (iodotmt ). The irreversible labeling with this iodotmt reagent enables the enrichment and quantification of cysteine modifications such as S-nitrosylation, oxidation and disulfide bridges. Additionally, several new strategies for higher TMT reagent multiplexing will be described.. Glycroproteoics and Glycomics: Towards direct glycan/glycopeptide sequencing and identification in glycoproteomic applications Glycosylation is an important post-translational modification (PTM) that plays crucial roles in biochemical processes. However, characterization and identification of glycoproteins and free glycans remains analytically challenging. We will present workflows that minimize some of the pain points associated with glycopeptide analysis. Strategies for enrichment will be discussed along with implementation of a novel acquisition strategy termed higher energy collision dissociation-accurate mass product dependent-electron transfer dissociation (HCD-PD-ETD) which increases the overall productivity of glycopeptides analysis. We also introduce a novel bioinformatics tool called Byonic to automate largescale data analysis from the combined fragmentation techniques. Additionally we will also focus on new workflows and tools for simplifying free glycan analysis. Discovery : Applying Q Exactive LC-MS-MS and sieve 2.0 software for cutting edge metabolomics and lipidomics research This talk will feature metabolomics and lipidomics workflows using the benchtop Q Exactive high resolution Orbitrap LC-MS/MS system and SIEVE 2.0 software. Example applications covered in the presentation are: (1) untargeted analysis of metabolites in serum from normal vs. Zucker diabetic fatty (ZDF) rats, a model for type II diabetes, and (2) untargeted analysis of lipids in wild-type yeast (S. cerevisiae) and a knockout (KO) strain that does not produce coenzyme Q (CoQ). The use of Isotope Ratio Outlier Analysis (IROA), a powerful new strategy for metabolomics analysis, is presented for metabolomics analysis in worms (C. elegans). The use of SIEVE software for processing complex metabolomics data is illustrated for analysis of serum from ZDF rats.

3 topic abstracts: Targeted Protein Quantification Using HR/AM Approach: A more selective and higher throughput method to precisely quantify your protein of interest High-resolution, accurate-mass (HR/AM) approach is emerging into one of the major quantitative techniques for targeted protein analysis because of its high selectivity, throughput and flexibility. The Thermo Scientific Q Exactive mass spectrometer combines high-performance quadrupole precursor selection with high-resolution, accurate-mass Orbitrap detection. It offers unique capabilities of parallel filling and detection, multiplexed detection and high resolving power up to 140,000 FWHM which enables accurate protein quantification with UHPLC comparable duty cycle. We will demonstrate the superior quantitative performance of Q Exactive by using both HR/AM SIM and HCD MS/ MS approaches. Targeted Qualitative and Quantitative Studies: Using mass spectrometry immunoassays (MSIA) enrichment strategies for microheterogeneity detection and quantitation* Targeted protein/peptide studies using LC-MS provide significant advantage for quantifying relative/absolute expression levels in complex matrices. To increase the detection capabilities, antibody-based enrichment strategies have been used. A known mono- or polyclonal antibody can be used to selectively capture a specific region of the targeted protein, resulting in simultaneous capture of multiple protein forms. Our approach is to utilize a pipette-tip based approach to bind antibodies for targeted protein capture. Mass Spectrometry Immunoassays (MSIA) enrichment has been shown to increase the detection and quantitation range of targeted proteins compared to magnetic bead based approach. The present approach focuses on PTH and the microheterogeneity across different classes of samples. To increase the robustness of the entire workflow, heavy labeled PTH was generated using in vitro translation (IVT). The heavy labeled PTH analog was spiked into each sample to determine capture efficiency, digestion reproducibility, and relative/ absolute quantitation. Reserve your seat at Space is limited. Free to attend * This talk is not available at all locations.

4 Tues, Jan 15, Ithaca, NY Agendas for 2012 Tour Wed, Jan 16, 2013 Baltimore, MD Welcome Guest Speaker - Robert N. Cole, Ph. D., Director, Mass Spectrometry and Proteomics Facility, Johns Hopkins School of Medicine, Quantitative Proteomics in Nutrition, Enyzme Kinetics and Protein Modifications using an Orbitrap Velos 10:00am 10:45am 10:45am 11:00am 11:00am 11:30am 12:00pm 1:00pm /Q&A 1:00am 1:45pm 2:30pm 3:00pm Tues, Nov 6, 2012 Kansas City, KS 10:00am 10:30am Wed, Nov 7, 2012 St. Louis, MO 1:00pm 1:30pm 1:30pm 1:45pm 1:45pm 2:15pm 2:15pm 2:45pm 2:45pm 3:00pm Snack 3:00pm 3:30pm 3:30pm 4:15pm /Lipodomics 4:15pm 4:30pm Thrs, Nov 8, 2012 Madison, WI 8:00am - 8:30am 8:30am - 8:45am 8:45am - 9:15am 9:15am - 9:45am 9:45am - 10:00am 10:00am - 10:30am 10:30am - 11:00am 11:00am - 11:30am 11:30am - 11:45am 11:45am * will be in a different location HR/AM Quan *

5 Frid, Nov 9, 2012 Cleveland, OH Agendas for 2012 Tour 9:30am 10: 00am Guest Customer Speaker - Dr. Belinda Willard, Cleveland Clinic, 2H2O Proteome Dynamics on an Orbitrap Elite LC-MS/ MS system 10:00am 10:15am 10:15am 10:45am 10:45am 11:15pm 11:15am - 12:00pm 12:00pm 1:00pm Tues, Nov 13, 2012 Louisville, KY 9:30am 10:00am Guest Speaker - Michael Merchant, Ph.D., Technical Director, Core Proteomics Laboratories, University of Louisville Medical School, : Leveraging the high-sensitivity and highresolution accurate mass capabilities of the LTQ-Orbitrap-Elite for identification of biomarkers for lupus nephritis 10:00am 10:15am 10:15am 10:45am 10:45am 11:15pm 11:15am - 12:00pm 12:00pm 1:00pm Wed, Nov 14, 2012 Bloomington, IN 12:00pm 1:00pm Thrs, Nov 15, 2012 Chicago, IL Frid, Nov 16, 2012 Rochester, MN 9:30am 10:00am 10:00am 10:15am 10:15am 10:45am 10:45am 11:15pm 11:15am - 12:00pm 12:00pm 1:00pm Guest Customer Speaker Reid Townsend