Temperature and ph Stability of a New High Temperature C18 Polydentate Silica Column

Transcription

1 Temperature and ph Stability of a New High Temperature C18 Polydentate lica Column Stephanie J. Marin 1, Brian A. Jones 1 Jody Clark 1, Nathan L. Porter 1 J. Andreas Lippert 2, and Todd M. Johnson 2 1. Selerity Technologies, Inc W. Custer Road Salt Lake City, UT Dept. of Chemistry Weber State University gden, UT

2 utline Benefits of durable silica columns Durable column chemistries Blaze 200 chemistry Selectivity comparison Blaze 200 evaluation 2

3 Benefits of ph Stable Columns Wide ph range Ion suppression for acids Ion suppression for amines Column regeneration by elution of contaminants at ph extremes Separating basic analytes at high ph gives: Increased loading Increased retention Increased resolution 3

4 Benefits of Temperature Stable Columns High thermal stability Selectivity tuning Faster analysis Less organic modifier required Wider temperature programming range Extended column lifetimes under more moderate conditions 4

5 lica Columns are Subject to Hydrolysis and Degradation Water attacks siloxane bond or behind point of phase attachment Backside Attack H 2: R Degradation of the lica Support CH 3 + H H H :H CH 3 H + 5

6 Durable Non-lica Column Chemistries Columns stable at high temperatures Zirconia columns Polymeric PSDVB or DVB columns Hamilton PRP-1 Jordi DVB Stable to 150 C ph 0 to 14 Graphitic Carbon Thermo Hypercarb Stable to 200 C ph 0 to 14 6

7 Comparison Between Durable lica Based Chemistries Sterically Hindered Phases Agilent Stablebond give good stability only with low ph at elevated temperatures rganic/inorganic hybrid family Waters Xterra Carbon/silica hybrid particle Stable chemistry, apparently usable at high ph and elevated temperatures Surface organic groups reduce low ph stability 7

8 Comparison Between Durable lica Selerity Blaze 200 TM Based Chemistries mple surface coating with dendrimeric reagents that can be readily purified Works on any silica and has traditional silica selectivity Underlying particle structure undisturbed, resulting in the highest strength and efficiency ne column has stability at low and high ph and high temperature 8

9 Blaze 200 TM Bonding Chemistry Generation 1 ethyl-bridged carbosilane dendrimers with multiple attachment points as barrier layers and functional layers Representative reagents: (CH 2 CH 2 Cl 3 ) 4 C 18 (CH 2 CH 2 Cl 3 ) 3 US Patent 6,794,044, and additional US and International Patents Pending 9

10 Selerity Blaze 200 TM Multiple Point Bonding H H H H H H H H H H H H H H lica Surface & Barrier Layer US and International Patents Pending 10

11 C 11 H 23 Functional Layer Added C 7 H 15 H H H H H H H H H H H H US and International Patents Pending 11

12 Selectivity Comparison Agilent SB, Xbridge and Blaze 200 TM mv SRM 870 test conditions were followed, and indicate typical C18 silica column selectivity. Uracil measures void volume. Toluene and ethylbenzene measure overall retention. Peak shape and elution of quinizarin indicate activity toward metal chelating agents. Tailing and asymmetry for amitriptyline indicates silanol interaction. 500 Detection: UV 254 nm Mobile Phase: 80:20 methanol:5mm potassium phosphate ph 7.0 Temperature: 23 C Minutes Waters Xbridge (150 x 3.5 mm) Blaze 200 (150 x 4.6 mm) Agilent SB C18 (150 x 4.6 mm) 12

13 Selectivity Comparison Blaze 200 TM C18 / Zorbax SB-C18 AU Analytes: Uracil, Phenobarbital, Butalbarbital, Carbromal Mobile Phase: 20:80 ACN:3mM phosphate at ph 7.0, 3 ml/min. Temperature: 60 C Columns: 4.6 x 150 mm Zorbax SB-C18 Blaze 200 TM C Minutes 13

14 Analgesics Blaze 200 TM C18 Blaze 200 TM 150 x 4.6 mm 30:70 Acetonitrile:water w/ 0.1% TFA 1.5 ml/min UV 220 nm 50 C to 140 C at 30 /min, hold for 10 minutes Elution order: Acetaminophen Caffeine Salicylamide Aspirin Salicylic acid Naproxen Ibuprofen Minutes 14

15 Selerity Polaratherm TM Series 9000 Total Temperature Controller Used in this study except where noted Forced air oven and chiller Isothermal and thermal gradient operation Sub-zero to 200 C Thermal gradients up to 30 C/min Mobile phase preheating and pre-cooling Peltier effluent temperature control Vapor sensor Compatible with any HPLC system Integrated software control with Waters, Agilent, and EZChrom 15

16 Effects of Temperature on the Theoretical Plates of Acetophenone Peak 120% Methanol:Water Percentage of Initial Theoretical Plates 100% 80% 60% 40% 20% 100% Water 20% MetH 40% MetH 60% MetH 80% MetH 0% vernight Column Temperature in o C 16

17 Effects of Temperature and ph on the Theoretical Plates of Acetophenone Peak 60% Acetonitrile 120% Percentage of Initial Theoretical Plates 100% 80% 60% 40% 20% ph 2.0 ph 3.0 ph 9.0 ph 10.0 water 0% vernight Column Temperature in o C 17

18 High ph Stability Evaluation mau Column: Blaze 200 C18, 2.1 x 50 mm, 3 µm, 100A Mobile Phase: 50:50 ACN:50mM pyrrolidine at ph 12, Flow Rate: 0.8 ml/min Detection: UV at 254 nm Temperature: 40 C Analyte: Amitryptyline Red: Initial Blue: After 2500 column volumes at ph 12! Minutes 18

19 Availability of Columns High temperature, Blaze TM Selerity, RIC ph extreme, phidelity TM Through Restek Corporation under license from Selerity Future work: Pore sizes, particle sizes, expanded chemistries 19

20 Conclusions Highly durable coating and protective layer lica columns with traditional selectivity stable at extended ph and temperature ranges 20

21 Acknowledgements Weber State University students Jarem B. Lloyd Jared P. Smith Bryce T. Johnson Jason Furlow Restek Corporation 21