MUHSIN JAMAL. Permanent Address: Village Gumbat Post Office Ziarat Talash Tehsil Timergara, Distt Dir (L) KPK, Pakistan EDUCATION

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1 MUHSIN JAMAL Assistant Professor, Department of Microbiology, Abdul Wali Khan University, Garden Campus, Mardan, Pakistan ID: Contact: Permanent Address: Village Gumbat Post Office Ziarat Talash Tehsil Timergara, Distt Dir (L) KPK, Pakistan EDUCATION 2015: PhD in Microbiology (Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences & Technology, Islamabad, Pakistan and University of Florida, USA. Grade: 1st Division: 3.92/4.00 CGPA Research Title: Isolation and Characterization of Bacteriophages and their Potential to Control Bacterial Biofilm. 2010: M. Phil in Virology and Immunology: Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences & Technology, Islamabad, Pakistan. Grade: 1 st Division: 3.60/4.00 CGPA Research Title: Detection of FLT-3 Internal Tandem Duplication in Patients with Acute Myeloid Leukemia. 2008: Bachelor of Biotechnology (Hons)- Department of Biotechnology, University of Malakand, KPK, Pakistan. Grade: 1 st Division: % Research Title: Characterization of wheat (Triticum aestivum l.) Varieties by SDS-PAGE 2003: Higher Secondary School Certificate- Board of Intermediate & Secondary Education, Malakand, KPK. Pakistan.

2 Grade: 1 st Division: % 2000: Secondary School Certificate- Board of Intermediate & Secondary Education, Swat, KPK. Pakistan. Grade: 1 st Division: % RESEARCH INTEREST Bacteriophage therapy and Bacterial control: Interested in Isolation of bacteriopges from various sources against various multidrug resistant bugs (MDR Bacteria) and studying various characteristics of such phages having a key role in phage therapy. Biofilm study: Also interested in studying various bacterial strains tendency for biofilm formation on various material surfaces and also control of such a biofilm using host specific bacteriophages and phage cocktails. Bacterial antibiotic resistance: Studying drug resistance in different bacterial isolates from local hospitals, especially focusing on molecular level along with other factors. AWARDS/ SCHOLARSHIPS Merit Scholarship (Ministry of Science and Technology, Pakistan) - ( )- PhD,Virology and Immunology- NUST Center of Virology and Immunology, National University of Sciences & Technology, Islamabad, Pakistan. Merit Scholar Ship: HEC PhD Fellowship for 5000 Scholars Phase II ( ) IRSIP Scholarship during PhD funded by HEC. Major Courses studied (Graduate & Post-Graduate) Microbiology Medical Microbiology Food Microbiology Environmental Microbiology General Virology Medical Virology Toxicology

3 Cell Biology Molecular Biology Biochemistry Phytochemistry Molecular Genetics Bacterial Genetics Enzymology Biopharmaceuticals Immunology Vaccinology General Virology Advances in Virology Research Techniques Bioinformatics Health Biotechnology Environmental Biotechnology Industrial Biotechnology Microbial Biotechnology (More detailed subject list can be provided) RESEARCH HANDLINGS/ TECHNIQUES Bacteriophage isolation and purification Bacteriophage typing Bacterial various culturing techniques Bacterial various preservation techniques Slant, Stab, Glycerol stock method Bacterial biofilm formation by different Methods e.g. Tube method, 96-well and Congo Red method DNA/RNA Extractions Polymerase Chain Reaction (PCR) Reverse Transcription (RT) Spectrophotometeric Analysis

4 Gene Cloning Transformations (Gene ) DNA Restriction Analysis SDS PAGE (Protein) Primer Designing Technique Transmission Electron Microscopy (TEM) Sample preparation, Fixation, loading Scanning Electron Microscopy (SEM) Sample preparation, Fixation, loading RESEARCH EXPERIENCE o Six months research experience at Emerging Pathogens Institute (EPI), University of Florida (UF), Florida, USA (Aug Feb-2014) o Four year research experience at the Microbiology Lab, Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), Islamabad, Pakistan (06/ /2013) o Six months research experience at the Department of Biotechnology, University of Malakand (UOM), Chakdara, Dir lower (11/2007-4/2008) PUBLICATIONS (20) Waqas Nasir Chaudhry, Rabia Badar, Muhsin Jamal, Jason Jeong, Jamal Zafar, Saadia Andleeb (2016). Clinico-microbiological study and antibiotic resistance profile with meca and ESBL gene prevalence in diabetic foot infections. Experimental and Therapeutic Medicine. 11(3): (I.F: 1.3) (19) Muhammad Asif Nawaz, Zainab Bibi, Haneef Ur Rehman, Muhsin Jamal, Tour Jan, Afsheen Aman and Shah Ali Ul Qader (2016) Production of α-1,4-glucosidase from Bacillus licheniformis KIBGE-IB4 by utilizing sweet potato peel (I.F: 2.7) (18) Tahir Hussain, Muhsin Jamal, Faridah Nighat and Saadia Andleeb (2016). Dissemination Of SHV Beta-Lactamase Genes Amongst Clinical Acinetobacter Baumannii Isolates.

5 Value in Health 19(3):A213 (IF: 3.3). (17) Muhsin Jamal, Tahir Hussain, Chythanya Rajanna Das and Saadia Andleeb (2015). Isolation and Characterization of a Myoviridae MJ1 Bacteriophage Against Multi- Drug Resistant Escherichia coli 3. Jundishapur Journal of Microbiology. 8(11): e (IF:.387) (16) Muhsin Jamal, Tahir Hussain, Chythanya Rajanna Das and Saadia Andleeb (2015). Characterization of Siphoviridae phage Z and studying its efficacy against multi-drug resistant (MDR) Klebsiella pneumonia planktonic cells and biofilm. Journal of Medical Microbiology. 64(4): (IF:2.3) (15) Muhsin Jamal, Waqas Nasir Chaudhry, Tahir Hussain, Chythanya Rajanna Das and Saadia Andleeb (2015). Characterization of new Myoviridae bacteriophagewz1 against multi-drug resistant (MDR) Shigella dysenteriae. Journal of Basic Microbiology. 55(4): (IF: 1.9). doi: /jobm (14) Muhsin Jamal, Tahir Hussain, Chythanya Rajanna Das and Saadia Andleeb (2015). Inhibition of clinical multi-drug resistant Klebsiella pneumoniae biofilm by Siphoviridae bacteriophage Z. Sci. Letters. 2015; 3(2): (13) Muhsin Jamal, Ufaq Tasneem, Tahir Hussain and Saadia Andleeb (2015). Bacterial Biofilm: Its Composition, Formation and Role in Human Infections. Research & Reviews: Journal of Microbiology and Biotechnology. 4(3):1-14 (12) Tahir Hussain, Muhsin Jamal, Tayyab-ur-Rahman and Saadia Andleeb (2015). Dengue: pathogenesis, prevention and treatment - A mini review. Advances in Life Sciences. 2(2): (11) Ummar Raheel, Muhsin Jamal and Najam-us-Sahar Sadaf Zaidi (2015). A Molecular Approach Designed to Limit the Replication of Mature DENV2 in Host Cells. Viral Immunology. 28(7):1-7 (I.F: 1.62) (10) Waqas Nasir Chaudhry, Rabia Badar, Muhsin Jamal, Jason Jeong, Jamal Zafar, Saadia Andleeb (2015). Clinico-microbiological study and antibiotic resistance profile with meca and ESBL gene prevalence in diabetic foot infections. Experimental and Therapeutic Medicine. 11(3): (I.F: 1.3) (9) Tahir Hussain, Muhsin Jamal, Faridah Nighat and Saadia Andleeb (2015). Increasing prevalence of CTX-M, TEM and SHV beta-lactamases in clinical pathogenic E. coli

6 causes significant health and economic losses- a perspective from Pakistan. Value in Health, 18(3):A229. DOI: /j.jval (IF: 3.3). (8) Tahir Hussain, Muhsin Jamal, Faridah Nighat and Saadia Andleeb (2014). Broad Spectrum Antibiotics and Resistance in Non-target Bacteria: An Example from Tetracycline. Journal of Pure and Applied Microbiol., 8, (7) Tahir Hussain Muhsin Jamal and Saadia Andleeb (2014). Potential epidemics due to multi-drug resistant enterobacteriacae in Pakistan. Journal of Bacteriology and Parasitology. 5(4): 201. (6) Tahir Hussain, Muhsin Jamal, Faridah Nighat and Saadia Andleeb (2014). 3 rd Generation Cephalosporin Resistance in Klebsiella pneumoniae from Pus Samples. World Journal of Microbiology, 9 (4): (5) Tahir Hussain, Muhsin Jamal, Faridah Nighat and Saadia Andleeb (2014). Susceptibility Profile of Klebsiella pneumoniae Isolates from Pus to -Lactam/- Lactamase Inhibitor Combinations. World Journal of Microbiology. 9 (2): (3) Hajra Sadia, Muhsin Jamal, Attya Bhatti, Muhammad Ayaz Anwar, Rubina Tabassum Siddiqui, Ishtiaq Qadri (2011). "Paucity of FLT-3 internal tandem duplications in patients with AML, ALL and CML". Current Opinion in Biotechnology. 22: 201. (IF: 18.03) (2) Muhsin Jamal, Inam-ur-Rahman, Muhammad Asif Nawaz, Tahir Hussain, Samiullah, Muhammad Ali and Muhammad Shuaib (2013). Physiochemical and Nutritional Evaluation of Selected Wheat Varieties in Pakistan. Journal of Agricultural and Biological Science. 8 (2): (1) Muhammad Shuaib, Muhsin Jamal, Haji Akbar, Israr Khan and Rehmana Khalid (2010). Evaluation of wheat by polyacrylamide gel Electrophoresis. African Journal of Biotechnology. 9 (2): PAPER SUBMITTED Muhsin Jamal, Tahir Hussain, Chythanya Rajanna Das and Saadia Andleeb. (2015). Control of multi-drug resistant (MDR) Pseudomonas aeruginosa planktonic cells and biofilm by applying single phage versus phage cocktail. Microbiology -SGM). (under review)

7 . Muhsin Jamal, Tahir Hussain, Waqas Nasir Chaudhry, Chythanya Rajanna Das and Saadia Andleeb(2015). Isolation and Characterization of Phage MJ2 and Studying its efficacy against Multi-Drug Resistant (MDR) Enterobacter cloacae Planktonic Cells and Biofilm. Journal of Microbiology and Biotechnology. (Under review) Taskeen Raza, Saadia Andleeb, Muhsin Jamal, Sidra Rahmat Ullah and Khalid Mehmood. (2015). Bacteriophage control of vancomycin resistant Enterococcus faecium in milk, treated and untreated water. World Journal of Microbiology and Biotechnology. (Under review) PUBLISHED SEQUENCES Jamal M., Sadia, H, Anwar M.A, Raza, M, Ain, Q and Qadri, I (2010). "FLT3- gene, FMS like tyrosine kinase 3 gene (exon 14 and 15 DNA) (Acc No ) Jamal, M., Hussain, T., Rajanna, C. and Andleeb, S (2014) Escherichia coli LF 16S ribosomal RNA gene, partial sequence (Acc No. KF955544) Jamal, M., Hussain, T., Rajanna, C. and Andleeb, S (2014) Klebsiella pneumoniae strain S ribosomal RNA gene, partial sequence ( Acc No. KJ438818) Jamal, M., Hussain,T., Rajanna, C. and Andleeb, S (2014) Pseudomonas aeruginosa S ribosomal RNA gene, partial sequence. (Acc no. KJ438816) Jamal, M., Hussain, T., Rajanna, C. and Andleeb, S (2014) seudomonas_aeruginosa S ribosomal RNA gene, partial sequence. (Acc No. KJ438817) Jamal, M., Hussain, T., Rajanna, C. and Andleeb, S (2014.) Achromobacter xylosoxidans 16S ribosomal RNA gene, partial sequence. (Acc No. KJ438819) WORKSHOPS/SEMINARS/CONFERENCES ATTENDED International symposium: Emerging Pathogens Institute Presents Research Day at Emerging Pathogens Institute (EPI) University of Florida (USA) (January 2014). International symposium on Hepatitis B Virus: Emergence and Surveillance 2nd April 2013

8 National Symposium on Frontiers in Nanotechnology, organized by NUST Science Society (May 2012) 3 rd IPR National conference 2011, organized by Intellectual Property rights organization (IPO), Government t of Pakistan. One day workshop titled TIME MANAGEMENT, organized by Center for Counseling and Career Advisory (C3A), National University of sciences and Technology, Islamabad, Pakistan (April 2011). Workshop on Research Methodology organized by Professional Development Center (PDC), NUST (January 2011). Conference on "1st Annual Symposium on HCV Management in Pakistan" NCVI NUST Pakistan (2/10/2011-2/11/2011). Seminar on Bioinformatics and Pakistani Industries titled: Biology Meets Computers Organized by Department of Bioinformatics & Biotechnology International Islamic University, Islamabad (November, 2010). 5 th International Biotechnology Symposium, organized by university of Sindh, Pakistan (October, 2009) LANGUAGES English: Speak, read, write and interpret. Urdu: Speak, read, write and interpret. Pashtu: Speak, read, write and interpret. REFERENCES (1) Dr. Saadia Andleeb Assistant Professor, Atta ur Rahman School of Applied Biosciences, (ASAB)

9 National University of Sciences & Technology. Islamabad, Pakistan. Phone office: Fax Number: Cell No: ID: (2) Dr. Najam us Sahar Sadad Zaidi Assistant Professor,Head of the Department, Industrial Biotechnology, Atta ur Rahman School of Applied Biosciences (ASAB), National University of Sciences & Technology. Islamabad,Pakistan. Phone office: Fax Number: Cell No: ID: (3) Dr. Chythanya Rajanna Das Senior Research Scientist Intralytix,(Biotechnology), Baltimore, Maryland, USA. Cell No: ID: