UE Biopathologie et Biobanking
|
|
|
- Imogen Hood
- 6 years ago
- Views:
Transcription
1 UE Biopathologie et Biobanking Modalités de l examen Trois questions rédactionnelles de 45 min (coefficient 3/4) 30 QCM (coefficient 1/4) Présence obligatoire (relevé de présence) Programme 2012 Cellules tumorales circulantes Biobanque: pourquoi et comment? (I) Biobanque: pourquoi et comment? (II) Technique en biopathologie (I) Techniques en biopathologie (II) Techniques en biopathologie (III) Pathologie moléculaire et médecine personnalisée Microscopie électronique Exemple de recherche translationnelle (I) Exemple de recherche translationnelle (II)
2 UE Biobanking & Biopathology Setting up criteria (including biospecimen quality controls) to evaluate the activity of a biobank Toward an «impact factor» for biobanks? Paul Hofman Laboratory of Clinical and Experimental Pathology & Human Biobank Nice Sophia Antipolis University, France
3 How to define a «good» biobank? Which indicators? Indicators showing that samples can be of good value for a research project Indicators of good research productivity Other indicators of strong interest
4 How to define a «good» biobank? Which indicators? Indicators showing that samples can be of good value for a research project Indicators of good research productivity Other indicators of strong interest
5 Indicators showing that samples can be of good value for a research project Quality of the samples Quality of DNA Quality of RNA Quantity of nucleic acids Associated mirror sample for morphological control Associated biological fluids to tissue samples Quality of associated clinical data General data Specific data for the pathology Efficiency for updating the data (including the follow up of the patients)
6 Indicators showing that samples can be of good value for a research project Quality of the samples Quality of DNA Quality of RNA Quantity of nucleic acids Associated mirror sample for morphological control Asssociated biological fluids to tissue samples Quality of associated clinical data General data Specific data for the pathology Efficiency for updating the data (including the follow up of the patients)
7 Parameters impacting the quality of samples Pre analytical time Frozen procedure versus fixatives Sample size Percentage of tumor cells Area of necrosis Matched healthy sample available Bottle necks
8 Parameters impacting the quality of samples Pre analytical time Frozen procedure versus fixatives Sample size Percentage of tumor cells Area of necrosis Matched healthy sample available Bottle necks
9 ? From the surgical room to the pathology laboratory and the biobank: How to get an «easy road»??
10 Improving molecular medicine research, «omic» projects, and clinical practice Archive frozen tissues & Archive paraffin embedded tissues But. How to control the Pre-Analytical Time Intervals (the «PATI»)? Impact on quality
11 Time Physicians Pathology Laboratory Biobanking Differences in tissue preservation Pre-Analytical Time Intervals Surgery Warm ischemia Hospital Organization Cold ischemia Transport to Pathology department How to avoid a labyrinth? Formalin New fixatives? Fixation Grossing Embedding Archive Sec-Hrs Sec-Hrs-Days Hrs-Days Days-Years
12 Time Physicians Pathology Laboratory Biobanking Differences in tissue preservation Pre-Analytical Time Intervals Surgery Warm ischemia Hospital Organization Cold ischemia Transport to Pathology department Bottle neck Need to get a visual control Frozen samples Visual control Archive Freezers Sec-Hrs Sec-Hrs-Days Hrs-Days Days-Years
13 The best and simple system: A pneumatic tube RFID technology Cold ischemia time control
14 Biobank Pathology Laboratory Surgery Surgical room Biobank Area Surgical Pathology Area ENTRY Pneumatic tube Embedding Recording data Meeting room ENTRY Laundry Immunohistochemistry Cytology Gross macroscopy Tissue specimen sectioning room Integrative pathology model Optimization of the process
15 Parameters impacting the quality of samples Pre analytical time Frozen procedure versus fixatives Sample size Percentage of tumor cells Area of necrosis Matched healthy sample available Bottle necks
16 FFPE sample DNA and mirna Frozen sample RNA and mirna
17 FFPE sample Frozen sample DNA and mirna RNA and mirna
18 Frozen sample procedure Drawbacks Ø Different times of cold ischemia Ø Morphological control in a mirror sample Ø Quite expensive equipment (nitrogen tanks, freezers) and safety environment Usefulness & Advantages Ø An easy and fast way for DNA & RNA extraction Ø DNA & RNA of very good quality
19 Formalin-fixed paraffin embbeded sample procedure Drawbacks Ø Variability for fixative time period Ø DNA fragmentation Ø Quality control is difficult and take quite a long time Ø RNA of very low quality Usefulness & Advantages Ø Available in all surgical pathology laboratories Ø An easy way for morphological control before DNA extraction
20 Indicators showing that samples can be of good value for research Quality of the samples Quality of DNA Quality of RNA Quantity of nucleic acids Associated mirror sample for morphological control Associated biological fluids Quality of associated clinical data General data Specific data for the pathology Efficiency for updating the data (including the follow up of the patients)
21 DNA extraction Ø Depending of sample conditionment: Ø Frozen tissue or Ø FFPE Ø Hand made method (lysis buffer & protease) Ø Kits (ex Qiagen) Ø Automated method If tiny material available: Hand made method If large material available: Automated method
22 DNA quality Ø PCR using a gene control Ø Gene control must be selected according to the sample Ø FFPE: <350 pb (S26 gene) Ø Frozen sample: > 350 pb (GAPDH gene) C- MW C+ Ø DNA gel migration
23 DNA quantification (spectrophotometer) Ø Analysis of 260/280 & 260/230 ratios Ø Potential proteins and/or solvants contamination DNA Solvant Protein
24 Indicators showing that samples can be of good value for a research project Quality of the samples Quality of DNA Quality of RNA Quantity of nucleic acids Associated mirror sample for morphological control Associated biological fluids Quality of associated clinical data General data Specific data for the pathology Efficiency for updating the data (including the follow up of the patients)
25 RNA extraction Ø Quality of sample Ø Frozen tissue Ø Hand made (Trizol) Ø Different kits (ex Qiagen) Ø Automated method If tiny material available: Hand made method If large material available: Automated method
26 RNA quality & quantity (Bioanalyser Agilent) Ø RNA Integrity number (RIN) 7
27 Storage of nucleic acid collections Ø -80 C & -150 C freezers
28 Frozen samples Tumoral tissue 1R mg 1R mg 2R mg 3R mg 4R mg RFID : mg Matched healthy tissue THYROID / LUNG / HEAD & NECK / 1R SKIN mg 1R mg N Biobank LB11-2R mg N Pathology Lab LH11-3R mg Name : 4R mg Surname : RFID : mg Date : Artery clamping : min Cold ischemia : min Technician name : Surgical Pathologist name:
29 Parameters impacting the quality of samples Pre analytical time Frozen procedure versus fixatives Sample size Percentage of tumor cells Area of necrosis Matched healthy sample available Bottle necks
30 Tissue sampling (& other biospecimen sampling) «Time is moving» Tissue sample available for biobanking will become more and more smaller in the futur Fine needle aspiration Transbronchial biopsie Etc., Etc.
31 FNA 1 FNA 2 FNA3 Surgical How to collect in the future tiny tissue specimens for research projects? Large variabilty of the size (and wheight) of the different FFPE and frozen samples available for biobanking and research
32 Parameters impacting the quality of samples Pre analytical time Frozen procedure versus fixatives Sample size Percentage of tumor cells Area of necrosis Matched healthy sample Bottle necks
33 Variability of the percentage of tumor cells According to the tumor According to the tumor area 100% of tumor cells 5 % of tumor cells
34 Parameters impacting the quality of samples Pre analytical time Frozen procedure versus fixatives Sample size Percentage of tumor cells Area of necrosis Matched healthy sample available Bottle necks
35 Large necrosis 0% of tumor cells Lung specimen?
36 Parameters impacting the quality of samples Pre analytical time Frozen procedure versus fixatives Sample size Percentage of tumor cells Area of necrosis Matched healthy sample available Bottle necks
37 Indicators showing that samples can be of good value for a research project Quality of the samples Quality of DNA Quality of RNA Quantity of nucleic acids Associated mirror sample for morphological control Associated biological fluids Quality of associated clinical data General data Specific data for the pathology Efficiency for updating the data (including the follow up of the patients)
38 Biobank Initial check list (available for 6877 patients) ID Consent Sample weights Morphological control DNA & RNA quality Associated fluids
39 Indicators showing that samples can be of good value for a research project Quality of the samples Quality of DNA Quality of RNA Quantity of nucleic acids Associated mirror sample for morphological control Asssociated biological fluids Quality of associated clinical data General data Specific data for the pathology Efficiency for updating the data (including the follow up of the patients & treatments)
40 Tumoral and non Tumoral Tissues Patient Consent Frozen specimen Tumoral/ non tumoral Research Pathology Gross macroscopy Histology Paraffin Blocks «in mirror» Clinical datas Treatment Outcome Controls Images RNA Quantity/ Quality DNA arrays RNG Tissue microarrays automatisation quantification Central database
41 How to define a «good» biobank? Indicators showing that samples can be of good value for a research project Indicators of good research productivity Other indicators of strong interest
42 Indicators of good research productivity Number of publications Biobank staff are authors Biobank staff are contributors Biobank staff are mentioned in the acknowledgment section or the biobank is cited in the article Impact factor of the journal Cumulative impact factor by year Cumulative impact factor by year and by collection Grants Obtained by the users of the biobank Obtained to support the biobank Patents Based on research supported by the biobank Others
43 How to define a «good» biobank? Indicators showing that samples can be of good value for a research project Indicators of good research productivity Other indicators of strong interest
44 Other indicators of strong interest Ethical standards Certification and accreditation QC/QA processes in place within the resource Training/competence of biobank staff Educational training program (Academic program) Official recognition from Regional/National/ International Health Institutions Other important indicators of biobank efficiency
45 Other indicators of strong interest Ethical standards Certification and accreditation QC/QA processes in place within the resource Training/competence of biobank staff Educational training program Official recognition from Regional/National/ International Health Institutions Other important indicators of biobank efficiency
46 Certification NF S & ISO 9001 Accreditation NF ISO &NF ISO is the international standard for medical laboratories (ISO Medical laboratories - Particular requirements for quality and competence). ISO is the general standard for testing and calibration laboratories (ISO General requirements for the competence of testing and calibration laboratories).
47 Other indicators of strong interest Ethical standards Certification and accreditation QC/QA processes in place within the resource Training/competence of biobank staff Educational training program Official recognition from Regional/National/ International Health Institutions Other important indicators of biobank efficiency
48 How to get a senior manager for biobanking!
49
50 Other indicators of strong interest Ethical standards Certification and accreditation QC/QA processes in place within the resource Training/competence of biobank staff Educational training program Official recognition from Regional/National/ International Health Institutions Other important indicators of biobank efficiency
51 «Marketing efficiency»
52 Criteria to get a high standing biobank Accreditation & Certification Quality of samples Biopsies ou pièces opératoires Ethical Standards Resarch Productivity Grants and financial supports Toward a high Bioresource Research Impact factor
53 «The main bottle neck»? Biobanker Surgical Pathologist
54
55 Biobank06 Team
Tech Note. Using the ncounter Analysis System with FFPE Samples for Gene Expression Analysis. ncounter Gene Expression. Molecules That Count
ncounter Gene Expression Tech Note Using the ncounter Analysis System with FFPE Samples for Gene Expression Analysis Introduction For the past several decades, pathologists have kept samples obtained from
TheraLin. Universal Tissue Fixative Enabling Molecular Pathology
TheraLin Universal Tissue Fixative Enabling Molecular Pathology TheraLin Universal Tissue Fixative Enabling Molecular Pathology Contents Page # TheraLin Universal Tissue Fixative 3 Introduction 5 Easy
ROUND TABLE ARISLA Milano 26 marzo 2014 Le BioBanche Nodo Italiano di Biobanking and Biomolecular Resources Research Infrastructure
ROUND TABLE ARISLA Milano 26 marzo 2014 Le BioBanche Nodo Italiano di Biobanking and Biomolecular Resources Research Infrastructure Marialuisa Lavitrano Università Milano-Bicocca Coordinatore BBMRI-Italia
celldatasci.com/rnastorm RNAstorm RNA Isolation Kit for FFPE Tissue Samples Sample Kit (20 extractions)
celldatasci.com/rnastorm info@celldatasci.com RNAstorm RNA Isolation Kit for FFPE Tissue Samples Sample Kit (20 extractions) Support: Email: support@celldatasci.com Phone: 650.285.2376 (option 2) Toll-free:
Accessible answers. Targeted sequencing: accelerating and amplifying answers for oncology research
Accessible answers Targeted sequencing: accelerating and amplifying answers for oncology research Help advance precision medicine Accelerate results with Ion Torrent NGS Life without cancer. This is our
HIGH SCHOOL STUDENT SCIENCE WEEK. St. Paul s Hospital Vancouver, BC
HIGH SCHOOL STUDENT SCIENCE WEEK St. Paul s Hospital Vancouver, BC Sponsors 2 AGENDA Location: UBC James Hogg Research Centre (JHRC), St. Paul s Hospital, Room 166 Burrard Building, 1081 Burrard Street,
BEST PRACTICES IN MOUSE TISSUE SAMPLE PREPARATION FOR RNA EXTRACTION WITH PRECELLYS EVOLUTION
BEST PRACTICES IN MOUSE TISSUE SAMPLE PREPARATION FOR RNA EXTRACTION WITH PRECELLYS EVOLUTION The lab mouse is the most commonly used mammalian model system for genetic research. Scientists from a wide
Integrated Radiology & Pathology Diagnostic Services: The Time is Now at UCLA
Integrated Radiology & Pathology Diagnostic Services: The Time is Now at UCLA Scott W Binder MD Pritzker Professor of Clinical Pathology and Dermatology Senior Vice Chair Director, Pathology Clinical Services
ReliaPrep FFPE gdna Miniprep System
TECHNICAL MANUAL ReliaPrep FFPE gdna Miniprep System Instructions for Use of Products A2351 and A2352 Revised 12/15 TM352 ReliaPrep FFPE gdna Miniprep System All technical literature is available at: www.promega.com/protocols/
CAP Accreditation Checklists 2017 Edition
CAP Accreditation Checklists 2017 Edition The College of American Pathologists (CAP) accreditation checklists contain the CAP accreditation program requirements, developed on more than 50 years of insight
Optimized Covaris truxtrac FFPE RNA Protocol for Isolating RNA from Laser-Capture Microdissected (LCM) FFPE Tissue for Next Generation Sequencing
Optimized truxtrac FFPE RNA Protocol for Isolating RNA from Laser-Capture Microdissected (LCM) FFPE Tissue for Next Generation Sequencing Enni Markkanen Institute of Veterinary Pharmacology and Toxicology,
FFPE DNA Extraction kit
FFPE DNA Extraction kit Instruction Manual FFPE DNA Extraction kit Cat. No. C20000030, Format 50 rxns Version 1 / 14.08.13 Content Introduction 4 Overview of FFPE DNA extraction workflow 4 Kit contents
Developing an Accurate and Precise Companion Diagnostic Assay for Targeted Therapies in DLBCL
Developing an Accurate and Precise Companion Diagnostic Assay for Targeted Therapies in DLBCL James Storhoff, Ph.D. Senior Manager, Diagnostic Test Development World Cdx, Boston, Sep. 10th Molecules That
Lifespan MOLECULAR PATHOLOGY CORE LABORATORY SERVICE & FEE SCHEDULE (Effective 09/01/17) ArcturusXT Laser Capture Microdissection (LCM)
& FEE SCHEDULE (Effective 09/01/17) ArcturusXT Laser Capture Microdissection (LCM) : : Initial training includes LCM operation and $145.00 flat fee* No charge (first 4 hours; cap handling plus 3 hr free
Survey of Neural Networks in Digital Pathology and Pathology Workflow
Survey of Neural Networks in Digital Pathology and Pathology Workflow Christianne Dinsmore 1 1 Department of Computing and Digital Media DePaul University Chicago, IL Abstract Digital pathology has already
Product # Specifications. Kit Specifications Maximum Column Binding Capacity (gdna) Maximum Column Loading Volume.
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com FFPE DNA Purification Kit Product # 47400 Product Insert Norgen
CAP Laboratory Improvement Programs. A Call to Standardize Preanalytic Data Elements for Biospecimens
CAP Laboratory Improvement Programs A Call to Standardize Preanalytic Data Elements for Biospecimens James A. Robb, MD; Margaret L. Gulley, MD; Patrick L. Fitzgibbons, MD; Mary F. Kennedy, CT(ASCP), MPH;
Making More Efficient Use of Biospecimens. ISBER Contributions and Initiatives
Making More Efficient Use of Biospecimens. ISBER Contributions and Initiatives Members of the ISBER Education and Training Committee a,1, Members of the ISBER Science Policy a,1, Members of the ISBER Biospecimen
The Children s Hospital of Philadelphia Department of Pathology and Laboratory Medicine
TheChildren shospitalofphiladelphia DepartmentofPathologyandLaboratoryMedicine Muscle Biopsy - General Instructions The Division of Neuropathology, Department of Pathology and Laboratory Medicine, Children
Sample Flexibility for Gene Expression Analysis with the ncounter Analysis System
Technical Note Sample Flexibility for Gene Expression Analysis with the ncounter Analysis System Sensitive Gene Expression Analysis From Crude Preparations and Fragmented RNA Cell Lysates Tissue Homogenates
Quick Ray Manual Tissue Microarrayer. Manual Tissue Microarrayer Quick-Ray
Manual Tissue Microarrayer Quick-Ray 1 NOTE This user guide describes the instruction of Quick Ray. Review this user guide to avoid injury and prevent damage to this product or any products connected to
Notice of Faculty Disclosure
Pathologist Extenders: Current and Future Amy Clayton, M.D. Vice Chair Clinical Practice and Quality Department of Laboratory Medicine and Pathology Mayo Clinic, Rochester MN Notice of Faculty Disclosure
QuickExtract FFPE RNA Extraction Kit
QuickExtract FFPE RNA Extraction Kit Cat. Nos. QFR82805 and QFR82050 Connect with Epicentre on our blog (epicentral.blogspot.com), Facebook (facebook.com/epicentrebio), and Twitter (@EpicentreBio). www.epicentre.com
International standard for Biobanking
International standard for Biobanking Georges Dagher Inserm, BBMRI Paris, France 20/10/2017 GBW meeting 1 Increase quality Decrease Cost Take home message 20/10/2017 GBW meeting 2 Most of our current knowledge
in-situ PCR Presented for: Presented by: Date:
in-situ PCR Presented for: Presented by: Date: 2 in situ Hybridization - Definition in situ PCR is a method in which the polymerase chain reaction actually takes place in the cell on a slide, and the product
University of New Mexico Health Sciences Center Office of Research Policy for the Oversight of Human Tissue in Research
1. Applicability This policy applies to all tissues collected from volunteers and from nonliving human subjects for the purpose of research. Oversight of this policy and these tissues are the responsibility
ICH guideline E18 on genomic sampling and management of genomic data
1 2 3 22 January 2016 EMA/CHMP/ICH/11623/2016 Committee for Human Medicinal Products 4 5 6 ICH guideline E18 on genomic sampling and management of genomic data Step 3 Adopted by CHMP for release for consultation
ROUTINE SPECIMEN COLLECTION BY SPECIMEN TYPE
1. AUTOPSY EYE ROUTINE SPECIMEN COLLECTION BY SPECIMEN TYPE a. Arrangements: No special arrangements necessary. i. Immerse the specimen in a sufficient quantity of fixative so that the eye is covered completely
Evaluation with RNA ScreenTape Assays
Technical Overview Evaluation with RNA ScreenTape Assays Author Eva Graf Agilent Technologies, Inc. Waldbronn, Germany Abstract The success of library preparation for RNA sequencing workflows depends highly
Integrated Data Repository. Mike Conlon, PhD Associate Director, UF CTSI
Integrated Data Repository Mike Conlon, PhD Associate Director, UF CTSI What if we had a repository That contained clinical data from Epic for all patients in the health care system Combined that data
AmoyDx TM PIK3CA Five Mutations Detection Kit
AmoyDx TM PIK3CA Five Mutations Detection Kit Detection of five mutations in PIK3CA gene Instructions For Use Instructions Version: B1.3 Date of Revision: April 2012 Store at -20±2 1/5 Background Phosphatidylinositol
Unlocking your FFPE archive
Unlocking your FFPE archive Critical factors for molecular analysis of FFPE samples Sample & Assay Technologies Table of contents 1. 2. Introduction Important considerations when preparing and archiving
Use of BHQplus Probes to Optimize Multiplex Competitive qrt-pcr in Diagnostics. Dr. James C. Willey Chief Medical Advisor Accugenomics, Inc.
Use of BHQplus Probes to Optimize Multiplex Competitive qrt-pcr in Diagnostics Dr. James C. Willey Chief Medical Advisor Accugenomics, Inc. Professor, Medicine and Pathology University of Toledo College
Total RNA Purification Micro Kit Product # 35300
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Total RNA Purification Micro Kit Product # 35300 Product Insert
truxtrac FFPE DNA microtube Kit Column Purification (25)
PROTOCOL truxtrac FFPE DNA microtube Kit Column Purification (25) Adaptive Focused Acoustics (AFA) -based DNA extraction and purification from Formalin-Fixed, Paraffin-Embedded Tissue using columns Product
Streamline Pre-Analytic Orchard Pathology Processes
Orchard Pathology Orchard Pathology is a complete diagnostic information system where clinical and anatomic data share the same database and where pathology data is stored in discrete data fields to simplify
MMLV Reverse Transcriptase 1st-Strand cdna Synthesis Kit
MMLV Reverse Transcriptase 1st-Strand cdna Synthesis Kit Cat. No. MM070150 Available exclusively thru Lucigen. lucigen.com/epibio www.lucigen.com MA265E MMLV Reverse Transcriptase 1st-Strand cdna Synthesis
Determinants of RNA Quality from FFPE Samples
Determinants of RNA Quality from FFPE Samples Silke von Ahlfen 1, Andreas Missel 1, Klaus Bendrat 2, Martin Schlumpberger 1 * 1 QIAGEN GmbH, Hilden, Germany, 2 Gemeinschaftspraxis für Pathologie Niendorf
(Spin Column) For purification of DNA and RNA from formalin-fixed, paraffin-embedded tissue sections. Instruction for Use
AmoyDx FFPE DNA/RNA Kit (Spin Column) For purification of DNA and RNA from formalin-fixed, paraffin-embedded tissue sections Instruction for Use Instruction Version: B1.3 Revision Date: September 2015
E.Z.N.A. Tissue DNA Kit. D preps D preps D preps
E.Z.N.A. Tissue DNA Kit D3396-00 5 preps D3396-01 50 preps D3396-02 200 preps August 2016 E.Z.N.A. Tissue DNA Kit Table of Contents Introduction and Overview...2 Yield and Quality of DNA...3 Illustrated
EXTRACTME Kits, Product Brochure
EXTRACTME Kits, Product Brochure Interest in any of the products, request or order them at Bio-Connect. Bio-Connect B.V. T NL +31 (0)26 326 44 50 T BE +32 (0)2 503 03 48 Begonialaan 3a F NL +31 (0)26 326
Improved Sensitivity for FFPE Microarray Analysis: ArrayGrade FFPE RNA Isolation
Improved Sensitivity for FFPE Microarray Analysis: ArrayGrade FFPE RNA Isolation Improved RNA Isolation from FFPE Blocks and Slides for Greater Microarray Sensitivity By Savita Prabhakar, George Quellhorst,
Can/How do we put data intensive biology in the picture?
www.crs4.it Can/How do we put data intensive biology in the picture? 1 www.crs4.it Centro di Ricerca, Sviluppo e Studi Superiori in Sardegna Interdisciplinary research center focused on computational sciences
NRAS Codon 61 Mutation Analysis Reagents
NRAS Codon 61 Mutation Analysis Reagents User Manual V1.0 Cat No. GP19 32 reactions 1 CONTENTS Introduction 4 Overview of Mutector TM Assay 5 Materials Provided 6 Materials Required 7 Equipment Required
Plant/Fungi Total RNA Purification Kit Product # 25800, 31350, 25850
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Plant/Fungi Total RNA Purification Kit Product # 25800, 31350,
Protocol for DNA extraction from FFPE Samples
25, ave Georges Lemaître - B-6041 Gosselies (Belgique) Tel : +32 (0)71 37 85 27 - Fax : +32 (0)71 34 78 79 Protocol for DNA extraction from FFPE Samples Step 1. Paraffin Removal 1 Equilibrate a heat block
EZ-10 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK
EZ-0 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK (Bacteria, Plant, Animal, Blood) Version 8 Rev 05/0/03 EZ-0 Genomic DNA Kit Handbook Table of Contents Introduction Limitations of Use Features Applications
QUALITY CONTROL/QUALITY ASSURANCE IN THE MOLECULAR MICROBIOLOGY LABORATORY
QUALITY CONTROL/QUALITY ASSURANCE IN THE MOLECULAR MICROBIOLOGY LABORATORY Richard L. Hodinka, Ph.D. University of South Carolina School of Medicine Greenville Greenville Health System, Greenville, SC
Boost turnaround time for faster diagnosis
Boost turnaround time for faster diagnosis Tissue-Tek Xpress x120 Rapid Tissue Processor True rapid continuous tissue processing The Tissue-Tek Xpress x120 is a standardized, turnkey solution combining
Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System) Product # 62500
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Cells and Tissue DNA Isolation 96-Well Kit (Magnetic Bead System)
NRAS Mutation Analysis Reagents (Codons 12 and 13)
NRAS Mutation Analysis Reagents (Codons 12 and 13) User Manual V1.1 Cat No. GP18 32 reactions 1 CONTENTS Introduction 4 Overview of Mutector TM Assay 5 Materials Provided 6 Materials Required 7 Equipment
MICROARRAYS+SEQUENCING
MICROARRAYS+SEQUENCING The most efficient way to advance genomics research Down to a Science. www.affymetrix.com/downtoascience Affymetrix GeneChip Expression Technology Complementing your Next-Generation
3.1.4 DNA Microarray Technology
3.1.4 DNA Microarray Technology Scientists have discovered that one of the differences between healthy and cancer is which genes are turned on in each. Scientists can compare the gene expression patterns
Aligning Lab Goals & Institutional Goals Laughlin Rice
Aligning Lab Goals & Institutional Goals Laughlin Rice Senior Administrative Director Department of Pathology & Laboratory Medicine The Children s Hospital of Learning Objectives To understand the institutional
Cells and Tissue DNA Isolation Kit (Magnetic Bead System) 50 Preps Product # 59100
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Cells and Tissue DNA Isolation Kit (Magnetic Bead System) 50 Preps
QIAGEN s NGS Solutions for Biomarkers NGS & Bioinformatics team QIAGEN (Suzhou) Translational Medicine Co.,Ltd
QIAGEN s NGS Solutions for Biomarkers NGS & Bioinformatics team QIAGEN (Suzhou) Translational Medicine Co.,Ltd 1 Our current NGS & Bioinformatics Platform 2 Our NGS workflow and applications 3 QIAGEN s
PREPARATION OF HISTOLOGICAL SPECIMENS
PREPARATION OF HISTOLOGICAL SPECIMENS Histo-techniques Preparation of tissue for microscopic examination Series of processes Ultimate aim to make tissue visible as it is Pathology Vs Anatomy Steps vary
Laboratory Assistant Suite
Laboratory Assistant Suite Manual V e r sion: 1.0 D a t e: 18/03/2015 Index 1. Introduction... 8 1.1. System requirements... 9 2. Basic concepts... 10 3. Authentication... 13 Basic concepts... 13 Registration...
ncounter Elements TagSets General Purpose Reagents PACKAGE INSERT Molecules That Count NanoString Technologies, Inc.
ncounter Elements TagSets General Purpose Reagents PACKAGE INSERT NanoString Technologies, Inc. 530 Fairview Ave N Suite 2000 Seattle, Washington 98109 www.nanostring.com Tel: 206.378.6266 888.358.6266
Gene Regulation Solutions. Microarrays and Next-Generation Sequencing
Gene Regulation Solutions Microarrays and Next-Generation Sequencing Gene Regulation Solutions The Microarrays Advantage Microarrays Lead the Industry in: Comprehensive Content SurePrint G3 Human Gene
PreAnalytiX Supplementary Protocol
PreAnalytiX Supplementary Protocol Purification of full-length proteins from sections of PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue This protocol describes using the Qproteome FFPE Tissue kit
AmoyDx PIK3CA Five Mutations Detection Kit
AmoyDx PIK3CA Five Mutations Detection Kit Detection of five mutations in PIK3CA gene Instruction for Use Instruction Version: B2.2 Revision Date: June 2016 Store at -20±5 Background For: ADx-PI01 Phosphatidylinositol
TECHNOLOGIES & SERVICES FOR THERAPEUTIC ANTIBODY DEVELOPMENT
Specialist in tissue analysis by Histology, Immunohistochemistry and In Situ Hybridization TECHNOLOGIES & SERVICES FOR THERAPEUTIC ANTIBODY DEVELOPMENT CONTENTS Histalim: who we are Our areas of expertise
Cytoplasmic & Nuclear RNA Purification Kit Product # 21000, 37400
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Cytoplasmic & Nuclear RNA Purification Kit Product # 21000, 37400
Tissue Tug of War: Issues in tissue procurement for research and banking
Tissue Tug of War: Issues in tissue procurement for research and banking Christopher A. Moskaluk M.D., Ph.D. University of Virginia Disclosures: No commercial interests. Federal and non-profit support
QIAGEN Supplementary Protocol: Isolation of genomic DNA from tissue using the QIAGEN-tip Storage of tissue samples.
QIAGEN Supplementary Protocol: Isolation of genomic DNA from tissue using the QIAGEN-tip 2500 This protocol is designed for the rapid, easy, and non-toxic preparation of up to 2 mg genomic DNA from not
E.Z.N.A. MicroElute Genomic DNA Kit. D preps D preps D preps
E.Z.N.A. MicroElute Genomic DNA Kit D3096-00 5 preps D3096-01 50 preps D3096-02 200 preps December 2013 E.Z.N.A. MicroElute Genomic DNA Kit Table of Contents Introduction...2 Kit Contents/Storage and Stability...3
Histology Pattern Recognition Software in Investigative Pathology
Histology Pattern Recognition Software in Investigative Pathology J. Webster, DVM, PhD, DACVP Laboratory of Cancer Biology and Genetics National Cancer Institute, Bethesda, MD Pathology Visions 2011 November
TIME IT S PRIME. chemagic TM Prime TM instrument Streamlined Walk-away Automation. For all Human Sample Materials. Primary Sample Handling
IT S PRIME TIME chemagic TM Prime TM instrument Streamlined Walk-away Automation. For all Human Sample Materials. Primary Sample Nucleic Acid Isolation Eluate Quantitation of Extracted Samples Assay Setup
Billing Contact: Phone: Research Funding: NIH Funded Industry Funded Department Funded Short Study Protocol Title:
Please submit this application to CPRS@mednet.ucla.edu at least 2-3 weeks prior to the beginning of the research for approval. All UCLA studies using patient medical record numbers/identifiers must be
TaKaRa MiniBEST Viral RNA/DNA Extraction Kit Ver.5.0
Cat. # 9766 For Research Use TaKaRa MiniBEST Viral RNA/DNA Extraction Kit Ver.5.0 Product Manual Table of Contents I. Description... 3 II. Components... 3 III. Storage and shipping... 3 IV. Preparation
Comprehensive Report on FFPE Extraction Methods
Technical Note Comprehensive Report on FFPE Extraction Methods Mutation detection based on DNA or RNA sequencing is becoming increasingly important in both human disease research and clinical settings.
Electrophoresis and the Agilent Bioanalyzer. Advanced Biotechnology Lab I Florida Atlantic University January 23, 2008
Electrophoresis and the Agilent Bioanalyzer Advanced Biotechnology Lab I Florida Atlantic University January 23, 2008 Introduction Electrophoresis is one of the most commonlyused methods of separating
GenUP Virus DNA/RNA Kit
Product Insert GenUP Virus DNA/RNA Kit LOT: See product label EXPIRY DATE: See product label ORDERING INFORMATION PRODUCT GenUP Virus DNA/RNA Kit CAT. NO. BR0701101 BR0701102 BR0701103 SIZE 10 preps 50
truxtrac FFPE DNA microtube Kit - Bead Purification (24)
truxtrac FFPE DNA microtube Kit - Bead Purification (24) Adaptive Focused Acoustics (AFA) -based DNA extraction and purification from Formalin-Fixed, Paraffin-Embedded (FFPE) Tissue using Magnetic Beads
Comparing the Quality of Fixation for Gel-based Formalin (Formagel) versus Traditional Liquid-Based Formalin for Immunohistochemistry
Comparing the Quality of Fixation for Gel-based Formalin (Formagel) versus Traditional Liquid-Based Formalin for Immunohistochemistry Brian H. Le, M.D., Reading Hospital Reviewed by Michael R. LaFrinere,
Chapter 8. Nucleic Acids Extraction from Laser Microdissected FFPE Tissue Sections. Renate Burgemeister. Abstract. 1. Introduction
Chapter 8 Nucleic Acids Extraction from Laser Microdissected FFPE Tissue Sections Renate Burgemeister Abstract Tissue heterogeneity is a common source of unsuccessful experiments. Laser capture microdissection
HighPrep Blood & Tissue DNA Kit
MAGBIO ACCELERATING genomic research HighPrep Blood & Tissue DNA Kit Manual Revision v1.1 Catalog Nos. HPBTS-D16, HPBTS-D96, HPBTS-D96X4 Genomic DNA isolation from 20-250 μl of blood, lysate of tissues,
STANDARD OPERATING PROCEDURE
Effective Date: 12/1/2011 Page 1 of 12 Approval Name/Title Signature Date Authors: Jessica Landis Trial Implementation Manager Immune Tolerance Network Mary C. Philogene Associate Director, QA And Cell
LightCycler 480 qpcr Tools. Meeting the Challenge of Your Research
LightCycler 480 qpcr Tools Meeting the Challenge of Your Research Find the Optimal LightCycler 480 Reagents for Your Research Application: Are you analyzing DNA DNA Nucleic acid isolation Manual processing
Bill Kabat and Carmen Irizarry of the ACTG/IMPAACT Lab Tech Committee. Network Name, Title Signature Date
Title: Dried PBMC Pellet Processing Standard Operating Procedure (LDMS specimen code = BLD/EDTA or ACD/PEL) Origination Date: 01 Jun 2004 Total Pages: 6 Effective Date: 29 Nov 2012 SOP Number LTC-SOP-59
EZ-10 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK
EZ-0 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK (Bacteria, Plant, Animal, Blood) Version 5.0 Rev 03/25/205 Table of Contents Introduction 2 Limitations of Use 2 Features 2 Applications 2 Storage 2
High Pure PCR Template Preparation Kit for preparation of 100 nucleic acid samples Cat. No
for preparation of 100 nucleic acid samples Cat. No. 1 796 88 Principle Cells are lysed during a short incubation with Proteinase K in the presence of a chaotropic salt (guanidine HCl), which immediately
E.Z.N.A. Total RNA Kit II. R preps R preps R preps
E.Z.N.A. Total RNA Kit II R6934-00 5 preps R6934-01 50 preps R6934-02 200 preps September 2015 E.Z.N.A. Total RNA Kit II Table of Contents Introduction...2 Illustrated Protocol...3 Kit Contents/Storage
Quality of Cancer RNA Samples Is Essential for Molecular Classification Based on Microarray Results Application
Quality of Cancer RNA Samples Is Essential for Molecular Classification Based on Microarray Results Application Pathology Author Jenny Xiao Agilent Technologies, Inc. Deer Creek Road MC U-7 Palo Alto,
QIAamp DNA FFPE Tissue Handbook
April 2010 QIAamp DNA FFPE Tissue Handbook For purification of genomic DNA from formalin-fixed, paraffin-embedded tissues Sample & Assay Technologies Trademarks: QIAGEN, QIAamp, MinElute, Qproteome, REPLI-g,
E.Z.N.A. Blood DNA Mini Kit. D preps D preps D preps
E.Z.N.A. Blood DNA Mini Kit D3392-00 5 preps D3392-01 50 preps D3392-02 200 preps January 2017 E.Z.N.A. Blood DNA Mini Kit Table of Contents Introduction and Overview...2 Illustrated Protocol...3 Kit Contents/Storage
Tissue Microarray Catalog
Tissue Microarray Catalog 2012 2013 The most complete tissue microarray product line you can choose from Manual TMA Kits Semi-Automatic Tissue Arrayer Fully Automated Tissue Microarray Builder 1 EZ-TMA
Coding Essentials for Laboratories 2017
Coding Essentials for Laboratories 2017 An Easy-to-Use Tool for Coding and Reimbursement Compliance Prepared and Published By: MedLearn Publishing A Division of Panacea Healthcare Solutions, Inc. 287 East
MEDICAL LABORATORY SCIENCE (MLS)
MEDICAL LABORATORY SCIENCE (MLS) Explanation of Course Numbers Courses in the 1000s are primarily introductory undergraduate courses Those in the 2000s to 4000s are upper-division undergraduate courses
ab Plant Chromatin Extraction Kit
ab156906 Plant Chromatin Extraction Kit Instructions for Use For isolating chromatin or DNA-protein complex from plants in a simple and rapid format This product is for research use only and is not intended
KCC Path-Core Page 1 of 5
Instructions for Sample preparation for Paraffin embedding PLEASE NOTE: There is no one-size-fits-all method of tissue preparation for all experimental designs. Before harvesting tissue, you need to assess
Preparation of tissues for study
Preparation of tissues for study HISTOLOGY : It is the branch of science which deals with the microscopic study of normal tissue HISTOPATHOLOGY : It is the branch of science which deals with the microscopic
Manual innuprep Micro RNA Kit
Manual Order No.: 845-KS-2030010 10 reactions 845-KS-2030050 50 reactions 845-KS-2030250 250 reactions Publication No.: HB_KS-2030_e_120116 This documentation describes the state at the time of publishing.
E.Z.N.A. Plant RNA Kit. R preps R preps R preps
E.Z.N.A. Plant RNA Kit R6827-00 5 preps R6827-01 50 preps R6827-02 200 preps August 2014 E.Z.N.A. Plant RNA Kit Table of Contents Introduction...2 Illustrated Protocol...3 Kit Contents and Storage...4
CATS RNA-seq v2 library preparation protocol on RNA from FFPE-samples
CATS RNA-seq v2 library preparation protocol on RNA from FFPE-samples INTRODUCTION The following protocol offers a streamlined solution for whole transcriptome sequencing studies from human/ mouse/rat
The 100,000 Genomes Project Genomics Collaboration Event Finnish Residence
The 100,000 Genomes Project Genomics Collaboration Event Finnish Residence Prof Mark Caulfield FMedSci Chief Scientist for Genomics England Barts Heart Centre William Harvey Research Institute Queen Mary
Supplementary File 3: DNA and RNA isolation
Supplementary File 3: DNA and RNA isolation Q-CROC-02 Biopsy protocol For the purposes of this protocol, four needle core biopsies (NCBs) of lymph node tissue are isolated from each patient using a 16G
Manual innuprep Virus DNA Kit
Manual Order No.: 845-- reactions 845-- reactions 845-- reactions Publication No.: HB_KS-_e_1 This documentation describes the state at the time of publishing. It needs not necessarily agree with future