ambr 15 cell culture The Industry Standard Cell Culture Micro Bioreactor System
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1 ambr 15 cell culture The Industry Standard Cell Culture Micro Bioreactor System
2 Bioreactor System for Cell Line Development and Process Optimization ambr 15 is an automated microscale bioreactor system that replicates classical laboratory scale bioreactors. The benchtop system comprises disposable micro bioreactor vessels, an automated workstation and user friendly software. ambr 15 offers automated parallel processing and control of 24 or 48 bioreactor experiments using just one operator. Designed to be installed in a standard laminar airflow biological safety cabinet for aseptic operation. ambr 15 provides efficient, consistent and scalable bioreactor experiments compared to classical laboratory bioreactors.* ambr 15 transforms the way scientists undertake cell line development in the 21 st century - Improves early clone selection decisions by providing a predictive bioreactor model with ph and DO control - Is the established industry standard with 50 systems installed in the top 20 biopharma companies - Increases lab productivity by managing multiple (24 or 48) bioreactor development experiments in parallel. - Reduces the cost per experiment by saving substantial amounts on facility space, capital, labour, media and consumables. - Helps create better cell lines faster by automating vessel set-up, feeding, base addition and sampling. - Simplifies adoption of Quality by Design (QbD) principles enabling large multifactorial experi mental designs (DoE) in real time. * See separate ambr 15 Performance Data Sheet 2
3 3
4 ambr 15 Combines Disposable Micro Bioreactor Vessels, an Automated Workstation and Easy-to-Use Software Liquid handling unit For media, feed and reagent addition plus sampling from the bioreactor vessels. Unit includes dual pipette heads, automated vessel decapper and plate delidder. Culture stations Each culture station can run up to 12 micro bioreactor vessels and controls temperature and impeller speed. Gas mixture is controlled and delivered independently to each vessel. 4
5 Sterile working environment System fits within most standard biological safety cabinets. Software Software enables easy experiment construction. It controls and monitors all experiments and records data and events with a full audit trail. Labware lid management Lids are automatically removed prior to liquid transfer and replaced afterwards. Disposable tips Sterile disposable tips are used to eliminate cross contamination. Two sizes of high grade tips are available to transfer liquid volumes accurately and precisely. Image shows ambr 15 cell culture, 24 vessel system 5
6 Functions ambr 15 automatically controls, feeds and samples 24 or 48 bioreactor vessels in parallel ambr 15 cell culture Fully automated parallel processing, control and evaluation of all bioreactor experiments with: Online monitoring and closed-loop control of ph and DO Independent control of: O 2 CO 2 N 2 for each vessel Full control of impeller speed and bioreactor temperature Optional integration with Beckman Coulter Vi-CELL XR or Cedex HiRes cell viability analysers ambr 15 cell culture micro bioreactor vessel Mimics the characteristics of a classical lab scale bioreactor to enable optimal cell growth and productivity: ph and DO sensors for continuous monitoring and control Integral impeller for rapid, efficient mixing Sparge tube delivers gas to the impeller mixing zone 10 to 15 ml culture volume with sample port to allow addition of reagents & feeds or removal of samples Micro bioreactor vessel The core disposable micro bioreactor technology includes sensors, stirring impeller, gas sparging and sample port. 1 Gas sparge tube 2 Sample port 3 ph and DO sensor spots 4 Impeller 4 6
7 Applications ambr 15 cell culture can be used as a microscale model to unblock bottlenecks in a range of development applications such as: Cell line selection Media development Media feed strategies Process optimization QbD and DoE studies 7
8 Sales and Service Contacts For further contacts, visit Europe America Germany Sartorius Stedim Biotech GmbH August-Spindler-Strasse Goettingen Phone Fax Sartorius Stedim Systems GmbH Robert-Bosch-Strasse Guxhagen Phone Fax France Sartorius Stedim FMT S.A.S. ZI des Paluds Avenue de Jouques CS Aubagne Cedex Phone Fax Sartorius Stedim France SAS ZI des Paluds Avenue de Jouques CS Aubagne Cedex Phone Fax Austria Sartorius Stedim Austria GmbH Franzosengraben Vienna Phone Fax Belgium Sartorius Stedim Belgium N.V. Leuvensesteenweg, 248/B 1800 Vilvoorde Phone Fax Hungary Sartorius Stedim Hungária Kft. Kagyló u Budakeszi Phone Fax Italy Sartorius Stedim Italy S.p.A. Via dell Antella, 76/A Antella-Bagno a Ripoli (FI) Phone Fax Netherlands Sartorius Stedim Netherlands B.V. Phone Fax filtratie.nederland@sartorius-stedim.com Poland Sartorius Stedim Poland Sp. z o.o. ul. Wrzesinska Kostrzyn Phone Fax Russian Federation LLC Sartorius ICR and LLC Biohit Uralskaya str. 4, Lit. B , Saint-Petersburg Phone Fax Spain Sartorius Stedim Spain, S.A.U. Avda. de la Industria, 32 Edificio PAYMA Alcobendas (Madrid) Phone Fax Switzerland Sartorius Stedim Switzerland AG Ringstrasse 24 a 8317 Tagelswangen Phone Fax U.K. Sartorius Stedim UK Ltd. Longmead Business Centre Blenheim Road, Epsom Surrey KT19 9 QQ Phone Fax Sartorius Stedim Biotech UK formerly TAP Biosystems York Way, Royston, Herts, SG8 5WY Phone Fax USA Sartorius Stedim North America Inc. 5 Orville Drive, Suite 200 Bohemia, NY Toll-Free Fax Argentina Sartorius Argentina S.A. Int. A. Ávalos 4251 B1605ECS Munro Buenos Aires Phone Fax Brazil Sartorius do Brasil Ltda Avenida Senador Vergueiro 2962 São Bernardo do Campo CEP SP- Brasil Phone Fax Mexico Sartorius de México S.A. de C.V. Circuito Circunvalación Poniente No. 149 Ciudad Satélite 53100, Estado de México México Phone Fax Asia Pacific Australia Sartorius Stedim Australia Pty. Ltd. Unit 5, 7-11 Rodeo Drive Dandenong South Vic 3175 Phone Fax China Sartorius Stedim Biotech (Beijing) Co. Ltd. No. 33 Yu an Road Airport Industrial Park Zone B Shunyi District, Beijing Phone Fax Sartorius Stedim Biotech (Beijing) Co. Ltd. Shanghai Branch Office 3rd Floor, North Wing, Tower 1 No Jin Ke Road Pudong District, Shanghai Phone Fax Sartorius Stedim Biotech (Beijing) Co. Ltd. Guangzhou Representative Office Unit K, Building 23 Huihua Commerce & Trade Building No. 80 Xianlie Middle Road Guangzhou Phone Fax India Sartorius Stedim India Pvt. Ltd. #69/2-69/3, NH 48, Jakkasandra Nelamangala Tq Bangalore, India Phone Fax Japan Sartorius Stedim Japan K.K. 4th Fl., Daiwa Shinagawa North Bldg. 8-11, Kita-Shinagawa 1-chome Shinagawa-ku, Tokyo, Japan Phone Fax Malaysia Sartorius Stedim Malaysia Sdn. Bhd. Lot L3-E-3B, Enterprise 4 Technology Park Malaysia Bukit Jalil Kuala Lumpur, Malaysia Phone Fax Singapore Sartorius Stedim Singapore Pte. Ltd. 1 Science Park Road, The Capricorn, #05-08A, Singapore Science Park II Singapore Phone Fax South Korea Sartorius Korea Biotech Co., Ltd. 8th Floor, Solid Space B/D, PanGyoYeok-Ro 220, BunDang-Gu SeongNam-Si, GyeongGi-Do, Phone Fax TAP Biosystems is now part of the Sartorius Stedim Biotech Group Specifications subject to change without notice. Publication No.: SBI1522-e Order No.: Ver
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