Education. Harvard T.H. Chan School of Public Health

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1 665 Huntington Ave., Bldg. 1, Room 1309, Boston, MA Office: Cell: Education Harvard T.H. Chan School of Public Health Boston, MA Postdoctoral Research Fellow September 2011 present Projects: 1.) Linking exposures of particles released from nano-enabled products to toxicology 2.) Genotoxicity of engineered nanomaterials 3.) Screening for chemical toxicity using cryopreserved precision cut lung slices Mentors: Joseph Brain and Philip Demokritou North Carolina Agricultural and Technical State University Greensboro, North Carolina PhD, Energy and Environmental Systems August 2011 Advisor: Leonard Williams NC A&T State University /Wake Forest University M.S. Cancer Biology Advisor: Suzanne Hess North Carolina Agricultural and Technical State University B.S. Biology Summary of Achievements Winston-Salem, North Carolina May 2004 Greensboro, North Carolina May 1999 ~ 2016 Hoffman Program on Chemicals and Health Pilot Grant Award Harvard T.H. Chan School of Public Health - Project title: "DELTA: Do repeated pulmonary exposures to aerosolized cosmetics alter redox status and lead to sensitization or adaptation." ~ 2015 Alonzo Yerby Postdoctoral Fellowship Harvard T.H. Chan School of Public Health ~ 2015 NIEHS Center for Environmental Health Mini-Pilot Grant Award Development of Predictive Toxicology Platform using Precision Cut Lung Slices - Harvard T.H. Chan School of Public Health ~ 2013 NIEHS Center for Environmental Health Mini-Pilot Grant Award Assessing the Genotoxicity of Engineered Nanomaterials Harvard T.H. Chan School of Public Health ~ ( ) NIH NHLBI Research Training Grant Fellowship Harvard T.H. School of Public Health ~ (2012-present) Joseph D. Brain Postdoctoral Fellowship Harvard T.H. Chan School of Public Health

2 ~ 2011 Merck Minority Training Travel Award American Thoracic Society Conference, Denver, Colorado ~ USDA Transdisciplinary Training Program in Functional Foods Fellowship /Kannapolis Scholar Research Experience Postdoctoral Research Fellow ~ Harvard T.H. Chan School of Public Health ~ Center for Nanotechnology and Nanotoxicology ~ (2011- present) Mentors: Demokritou/Brain Developed integrated methodology to monitor, sample, extract, and prepare released aerosols/particulate matter from incinerated nano-enabled products Assessed the toxicity and genotoxicity of released aerosols and engineered nanomaterials using in vitro, ex vivo, and in vivo approaches Supervised two doctoral students in planning/conducting animal and cell culture experiments. Managed several projects and successful in completing research objectives, publications and writing grants. Doctoral Candidate ~ North Carolina A&T State University ~ ( ) Advisor: Dr. Leonard Williams ~ Dissertation Title: An Omics approach and analysis of biogenic and anthropogenic nanoparticle exposure on airway epithelium" Performed in vitro evaluations of biogenic and anthropogenic particle exposures using swine and human airway epithelial cells Developed and initiated protocols for genomic and proteomic analysis using quantitative real time PCR, Western Blot, 2-D DIGE respectively Trained graduate and undergraduate students on aseptic technique, cell culture, and cytotoxicity assays. Graduate Intern/Lab Manager~ North Carolina A&T State University ~ ( ) Internship Project Enhancing Cell Viability through Biospecific Films: Preliminary Studies for the Development of a Bioreactor Designed and conducted biocompatibility studies using primary liver cells and HepG2 liver carcinoma cell lines cultured on functionalized conductive films Developed and optimized the usage of conjugation chemistry for the immobilization of biospecific ligands such as hepatocyte growth factor (HGF), epidermal growth factor (EGF), and transforming growth factor α (TGF-α) on conductive surfaces. Managed and trained two graduate students and one undergraduate student

3 Graduate Student/Researcher/Teaching Assistant ~ North Carolina A&T State University/ Wake Forest University ( ) Thesis: The Cytotoxic Effect of Essential Oils on Lung and Colon Cancer Cells Maintained tissue and bacterial cultures using aseptic technique. Designed radiation and essential oil combination experiments to detect potential radiosensitizers. Conducted cytotoxicity tests on lung (H460), colon (RKO), and normal cells (NIH3T3) using various anticancer agents. Trained in animal handling and anesthetization. Publications Watson, C., Singh, D., Pirela, S.V., Demokritou, P. Toxicological Implications of Released Particulate Matter during Thermal Decomposition of Nano-Enabled Thermoplastics (2016) (In preparation to be submitted to NanoImpact) Nelson, B., Watson-Wright, C., Ibuki, Y., Moreno-Villanueva, M.,Karlsson, H.L., Hendriks, G., Sims, C.,Singh, N., Doak, S.H. Emerging Metrology for High Throughput Nanomaterial Genotoxicology. Mutagenesis. accepted July 1, Watson, C., DeLoid, G., Pal, A., Demokritou, P. Buoyant Nanoparticles: Implications on Assessing Nano-Cellular Interactions. (2016) Small DOI: /smll Watson, C., Damiani, F., Ram-Moham, S., Donaghey, T., Lichtenstein, J., Rodrigues, S., de Moura Queiroz, P., Brain, J.D., Krishnan, R., Molina, R. Screening for chemical toxicity using cryopreserved precision cut lung slices. Toxicol. Sci. (2016) 150 (1): doi: / toxsci/kfv320 Watson, C., Pal, A., Pirela,SV., Singh,D., Chalbot,M.S.G., Kavouras,I., and Demokritou, P. (2015) Linking exposures of particles released from nano-enabled products to toxicology: An integrated methodology for particle sampling, extraction, dispersion and dosing. Toxicological Sciences. doi: /toxsci/kfv095 Watson, C., Molina, R., Louzada, A., Donaghey, T. Brain, JD. (2015) Effects of zinc oxide nanoparticles on Kupffer cell motility, bacterial clearance and liver function. International Journal of Nanomedicine. Watson, C.; Ge, J.; Cohen, J.; Pyrgiotakis, G.; Engelward, B.; Demokritou, P.(2014) A High Throughput Screening Platform for Engineered Nanoparticle Mediated Genotoxicity using CometChip Technology. ACS Nano 2014;8, DOI: /nn404871p. Sotiriou, G.A., Watson, C., Murdaugh, K.M., Darrah, T.H., Pyrgiotakis, G., Elder, A., Brain, J.D., Demokritou, P. (2014) Engineering Safer-by-Design, Transparent, Silica-coated ZnO Nanorods with Reduced DNA Damage Potential, Environ. Sci.: Nano in press, DOI: /C3EN00062A. Zhou, E. H., Watson, C., Pizzo, R., Cohen, J., Dang, Q., de Barros, P. M. F., Park, C. Y., Chen, C., Brain, J. D., Butler, J. P., Ruberti, J. W., Fredberg, J. J. and Demokritou, P. (2014). "Assessing the impact of engineered nanoparticles on wound healing using a novel in-vitro bioassay." Nanomedicine 2014;13, 1-13

4 Pirela, S.; Molina, R.; Watson, C.; Cohen, J.; Bello, D.; Demokritou, P.; Brain, J. (2013) Effects of Copy Center Particles on the Lungs: A Toxicological Characterization Using a Balb/C Mice Model. Inhal. Toxicol.2013, Pyrgiotakis, G., Bordini, A., Watson, C., Demokritou, P, Brain, J. (2014) A Chemical free, Nanotechnology-based Method for Airborne Bacterial Inactivation using Engineered Water Nanostructures. Environ. Sci.: Nano, DOI: /C3EN00007A. Faraque, M., Darkwa, K., Watson, C., Waterman, J., Kumar, D.(2012) Synthesis, structure, and biocompatibility of pulsed laser deposited TiN nanowires for implant applications. Journal of Biomedical Materials Research: Part A. 7: doi: /jbm.a Funding NSF Grant- Thermal decomposition/incineration of Nano-Enabled Products (NEPs): Environmental Health and Safety Implications Co-Investigator Presentations Watson, C. The Utility of the CometChip Technology for the Evaluation of Nanomaterial Mediated Genotoxicity. 46 th Annual Environmental Mutagenesis and Genomics Society Conference, New Orleans, LA (25/9/2015) Pal, A.*, Watson, C.*, Pirela, S., Sotiriou, G., Chalbot, M.C, Kavouras, I., Demokritou, P. Integrated methodology for toxicity evaluation of released particulate matter during life cycle studies and assessment of real world exposure scenarios. Sustainable Nanotechnology Organization (SNO) Conference 2014, Boston, MA (4-5/11/2014) Watson, C., J. Ge, J. Cohen, G. Pyrgiotakis, B. Engelward, P.Demokritou Development of a high throughput screening assay for assessing nanoparticle mediated DNA damage using the CometChip Technology Cancer Nanotechnology Gordon Research Conference 2013, West Dover, VT (14-18/7/2013) Watson, C., J. Ge, J. Cohen, G. Pyrgiotakis, B. Engelward, P.Demokritou Development of a high throughput screening assay for assessing nanoparticle mediated DNA damage using the CometChip Technology Sustainable Nanotechnology Organization (SNO) Conference 2013, Santa Barbara, CA (3-5/11/2013)

5 Teaching Experience Guest Lecturer Course: Introduction to Toxicology Fall 2014 Guest Lecturer Course: Human Physiology Fall Department of Biology, North Carolina A&T State University Greensboro, NC Teaching Assistant Course: Introduction to Microbiology Fall Semester 2003 Tutored students in various microbiology topics weekly. Graded homework and exams. Prepared wet lab and experiments weekly. Department of Biology, North Carolina A&T State University Greensboro, NC Teaching Assistant Course: Introduction to Zoology Spring Semester 2004 Responsible for teaching recitation for entire class. Developed weekly lesson plan for recitation class.