Inter-Regional Laboratory Agreement Laboratory Consumables

Transcription

1 Inter-Regional Laboratory Agreement Laboratory Consumables sterile non-pyrogenic DNA-free RNase-free ATP-free FREE delivery NO HIDDEN charges GUARANTEED IRLA discount

2 Contents Products Page Products Page Contact Details Delivery Service Level Agreement 3 Tissue Culture Flasks 20 Tubes 4 Tubes Cuvettes 5 Multiply PCR Plates 6 Micro Test Plates 8 Adhesive Sealing Tapes 9 Sarstedt Quality Symbols for Laboratory Products Biosphere Sterile Pipette Tips with Filter 11 Quality Tips with Filter 12 Quality Tips without Filter 13 Compatibility Table - Pipette Tips 14 Pipette Tips and Tip SystemBox 18 Tissue Culture Cell + Tissue Culture Products 21 Tissue Culture Products 22 Filtropur V Vacuum Filtration 26 Filtropur S / Filtropur L Filtration Units 27 CryoPure Storage System 28 Storage Boxes Transfer Pipettes 30 Micro Tubes Multiply PCR Reaction Tubes 31 miniperm 32 x-well lumox dish fl exiperm quadriperm 33 Serological Pipettes 34 Containers Petri dishes Inoculation Spreader and 50 ml Centrifuge Tubes NEW! Now centrifugation up to g** Dark blue graduations for improved visibility of transparent fluids Improved seal and reinforced wall thickness Sterile versions certified non-pyrogenic Tube with brown coloration (light protection) Tube with screw cap, e.g. for trace element analysis **The information is valid for liquids with a density of 1.06 being centrifuged at room temperature in an appropriate rotor containing inserts that are adapted to the conical base of the tube. 2

3 Contact Details Delivery Service Level Agreement Contact Details Sarstedt Ltd, 68 Boston Road, Beaumont Leys, Leicester LE4 1AW Supplier Contact Tel.: Fax.: Web: ing Contact Tel.: Fax.: To ensure orders are processed effi ciently and without delay, when making an order please provide the following information: Contact name Contact number Full delivery address Full invoice address number Product number and description Quantity required s can also be placed using most major purchasing and credit cards. Delivery Standard Delivery is free on all orders to mainland Great Britain. We use TNT for all deliveries; delivery is 2-3 working days from the receipt of orders. In cases of urgency, we offer a guaranteed next day delivery service on all ex stock items. Relevant additional charges are as shown in the table below: Service Level Price 2-3 Working Days (standard) Free of charge Next Day Pre am Additional 20 Next Day Pre Noon Additional 10 Saturday Delivery Additional 20 Service Level Agreement Sarstedt operate to the following target service levels;: Service Description Target Time taken to quote for special quotation within 3 days Percentage of core items out of stock <1% Percentage of orders not filled first time round <5% Percentage of core list lines delivered within 3 days 98% Percentage of other catalogue items delivered within 5 days 95% Percentage of orders completely delivered the first time within 1 day 95% Percentage of orders completely delivered the first time within 5 days 98% Percentage of orders completely delivered the first time within 10 days 98% Percentage of orders completely delivered the first time within 20 days 99% Percentage of orders completely delivered the first time within 21+ days 99.5% Collection of returned goods within 5 days Delivery, product and invoicing errors within 5 days Speed of complaint response (see note) within 5 days Note: Errors raised as a result of delivery, product on invoicing errors are usually dealt with within 5 working days. In the event of complaints of a technical nature that require detailed investigation it will take a little longer due to escalation processes. In any event the customer will be kept informed as to progress of the complaint. 3

4 Tubes Tube with round base, push cap assembled, sterile ml Material Length/Ø mm pcs./case Cap Colour PS 95/ /case Tube with round base, push cap ml Material Length/ Ø mm pcs./case Compatible Caps* PP 75/12 4,000/case , Tubes with assembled two-position ventilation cap 1. Position 1: cap lightly pushed on ventilated 2. Position 2: cap tightly pushed on sealed ml Material Length/ Ø mm PP 75/12 without print PP 100/ PP 100/16 Description pcs./case with printed graduation with printed graduation 25/bag, sterile 25/bag, sterile ind. wrapped, sterile 1,000/case /case /case Tubes with skirted round/fl at base, screw cap in HD-PE ml Material Length/ Ø mm PS 50/ PP 79/16 Description Neutral cap, enclosed Neutral cap, enclosed pcs./case 2,000/case ,000/case Tubes with conical base and screw cap ml Material Length/ Ø mm Description pcs./case PP 90/25 Assembled lid 500/case PP 90/ PP with printed graduation and writing space 115/28 Assembled lid, with label Red cap, assembled, sterile, non-pyrogenic 500/case /case Tubes with conical base, screw cap from HD-PE, with graduation and writing space ml Material Length/ Ø mm PP 114/ PP 114/ PP 120/ PP 120/17 Description Red cap, assembled, sterile, non-pyrogenic Red cap, assembled, sterile, non-pyrogenic Red cap, assembled, sterile, non-pyrogenic Red cap, assembled, sterile, non-pyrogenic 25/Styrofoam rack 300/case 25/bag 300/case 50/Styrofoam rack 500/case 50/bag 500/case

5 Tubes Cuvettes TC tubes, sterile, TC quality, clear polystyrene The pretreated polystyrene TC tubes with red screw cap are especially suitable for: Culturing small cell populations Culturing suspension or monolayer cultures Storage or transport of cell material The screw cap enables an open or closed growth environment for cell cultures ml Length/Ø mm Description /16 round base /bag /16 round base ,000/case /16 conical, skirted base Semi-micro cuvettes 10 x 4 mm, optical pathway 10 mm Length mm Polystyrene Acrylic Material Pcs./case 100/rack by mould cavity number 100/rack by mould cavity number 2,000/case ,000/case Cuvette 10 x 10 mm, optical pathway 10 mm, 4 ml Length mm Polystyrene Material Pcs./case 100/rack by mould cavity number 2,000/case UV Micro cuvettes Length mm LCH mm 100 pcs./styrofoam rack, 400/case mm 100 pcs./styrofoam rack (ind. sealed with lid) 400/box, certified DNA-/RNase-/protein-free

6 Multiply PCR Plates Each production batch is subjected to a highly sensitive PCR method to guarantee compliance with the individual quality criteria. Consequently, award of the PCR Performance Tested quality symbol to a production batch is subject to successfully passing PCR tests. Thanks to this quality grade, customers can use Sarstedt disposables in direct contact with reagents and sample material for PCR in their experimental work. Products awarded the PCR Performance Tested standard meet the following requirements: DNase-/RNase-free Human DNA-free PCR inhibitor-free Multiply PCR plates made from polypropylene Certifi ed by the PCR Performance Tested quality symbol Rapid temperature transfer due to thin-walled construction of wells Sealable with heat-resistant highly transparent tape or fl at lid strip 96 Well Multiply PCR plate, without lateral skirt Flat PCR plate fi ts most commonly used thermocyclers/dna sequencers Raised well rims for optimum sealing with adhesive tape Description/ max. Colour Compatible Instruments 96 Well/ 0.3 ml 5 pcs./bag 25/case e.g. Applied Biosystems, Biometra, Bio-Rad, Eppendorf, Ericom, MJ Research, Stratagene, Techne, Thermo Hybaid, Transgenomic Well White Multiply PCR plate optimised for qpcr Black alphanumeric print for enhanced well identifi cation Sealable with heat-resistant, highly transparent foil or fl at lid strip Special feature Optimised for qpcr Description Colour 96 Well, without skirt white 25 pcs./case Well Multiply PCR plates with lateral half skirt Recommended for Applied Biosystems thermocyclers/dna sequencers Can be sealed with heat-resistant, highly transparent tape or fl at lid strip Frame enables barcoding and application in automated systems Also available with standard 128 barcode Description/ max. Colour Compatible Instruments 96 Well/0.3 ml without barcode label 96 Well/0.3 ml with barcode label 25 pcs./case 25 pcs./case e.g. ABI Applied Biosystems, Biometra, Hybaid, MJ Research, Eppendorf

7 Multiply PCR Plates 96 well Multiply PCR plate with lateral skirt for robotic systems and real-time thermocyclers (e.g. MJ Research, Amersham Bioscience, Hybaid, Stratagene, Eppendorf, Bio-Rad, Techne) SBS compliant, with low profi le wells Sealable with heat-resistant highly transparent tape or fl at lid strip Frame enables barcoding and application in automated systems Description/ max. Colour Compatible Instruments Well/0.2 ml 25 pcs./case e.g. MegaBace 500/1000 DNA Analysis System, icycler, DNA Engine Opticon Well Multiply PCR plate with lateral half skirt, optimised for ABI Fast PCR Systems (ABI Prism 9800 and 7500 Fast) Low profi le design reduces dead volume and cycle times Ultra-clear thin-walled wells provide optimal signal recovery Suitable for most standard thermocyclers, e.g. from MJ Research Description/ max. Colour Compatible Instruments Well/0.2 ml 25 pcs./case e.g. ABI FAST PCR Systems, MJ Research Well Multiply PCR plate with robust frame SBS compliant PCR plate, with robust frame for most commonly used thermocyclers/dna sequencers Raised well rims for optimum sealing with adhesive tape Description/ max. Colour Compatible Instruments Well/40 μl 50 pcs./case e.g. ABI Prism 3100, 7700, GeneAmp 9700, Bio-Rad, MJ Research, Eppendorf Well Multiply PCR plate without skirt, in microtitre format Biosphere quality, individually wrapped sterile to avoid contamination Biosphere lid strips packed with 12 strips per bag are available separately (see below) Compatible with RackSystem storage and pipetting station Description ml Biosphere quality, sterile 1/bag 20/case Compatible Instruments e.g. Biometra, MJ Research, Eppendorf Multiply lid strip, optically clear, suitable for real-time PCR PCR Performance Tested Description NEW Strip of 8 lids for and Strips of 8 lids, flat, for 96 Well plates, highly transparent Strips of 8 lids, flat, for 96 Well plates, highly transparent 120 strips/bag 480 strips/case 120 pcs./bag 480 pcs./case 12 strips/bag 240 strips/case

8 Micro Test Plates Micro test plates from high-quality clear polystyrene Compatible with all common dispensers, readers and washers Excellent and consistent optical quality Optimal surface Uniform design Available with, U, und V shaped wells Micro test plates, PS, with 96 wells Well Shape Description without lid with lid, sterile without lid with lid, sterile without lid with lid, sterile 5/bag 100/case 1/bag 100/case 5/bag 100/case 1/bag 100/case 5/bag 100/case 1/bag 100/case Lid for micro test plates, PS /bag 150/case Adhesive fi lm for micro test plates Acetate foil (impermeable to air), -40 C to 100 C /case Terasaki micro test plate 60 Well plates, 10 μl (tissue culture treated) For use with micro volumes 10 Plates with lid/pack, gamma-sterile /case PS = polystyrene 8

9 Adhesive Sealing Tapes Transparent adhesive tape for PCR and sample storage The optically transparent foil is made from a 50 μm polyolefi n fi lm with perforated end tabs for easy removal. Optically transparent foil for standard and real-time PCR Removable without leaving any residue and ideally suited for sample storage to -70 C Description transparent 100 Tapes/case Aluminium foil for PCR and sample storage The heat-resistant, robust, pierceable 38 μm aluminium foil stands out for effi cient antievaporation protection and resistance to solvents. Perforated end tabs can be easily removed after attaching the foil. Tape can be pierced with probes and pipette tips Removable without leaving any residue Ideally suited for storing sample material/compounds to -70 C Description Aluminium 100 Foils/case

10 Sarstedt Quality Symbols for Laboratory Products Guaranteed reliability and reproducibility To accommodate the highly sophisticated needs of customers working in the fi eld of medical research, environmental technology, the pharmaceutical and food industries as well as in cellular and molecular biology, Sarstedt has developed the Biosphere quality products. The superior biological purity of sterile laboratory products is guaranteed by a controlled production process that complies with the strictest purity requirements so that our customers can be sure to achieve utmost reproducibility in their analyses and research experiments. Laborartikel All disposables, such as pipette tips and reaction tubes bearing the Biosphere symbol are sterile, DNA-, RNase- and ATP-free and non-pyrogenic. To reduce storage time of the sterile Biosphere products in open bags and inherent contamination risks, Biosphere tubes and plates are packaged in small quantities. Biosphere laboratory products are manufactured in accordance with defined production requirements and meet the following threshold values: DNA < 2 x g ATP < 3.5 x g RNase < 1.1 x Kunitz units Pyrogens < 2 x 10-3 EU Sterility is validated according to ISO PCR and other molecular-biological methods have gained access to laboratories all over the world. Sarstedt has enormously refined the production requirements for non-sterile PCR products. Skilled personnel, clean room conditions along with automated production processes enable contamination-free manufacturing. Each production batch is subjected to a highly sensitive PCR method to guarantee compliance with the individual quality criteria. Consequently, award of the PCR Performance Tested quality symbol to a production batch is subject to successfully passing PCR tests. Thanks to this quality grade, customers can use Sarstedt disposables in direct contact with reagents and sample material for PCR in their experimental work. Products awarded the PCR Performance Tested standard meet the following requirements: DNase-/RNase-free Human DNA-free PCR inhibitor-free 10

11 Biosphere Sterile Pipette Tips with Filter Certifi ed purity more than just sterile Highly sensitive methods like PCR are commonly applied in many fi elds of modern research and analytics today. A simple and safe measure to reduce carryover contamination is the use of pipette tips with integral fi lter. Precise positioning sterile non-pyrogenic DNA-free RNase-free ATP-free of the fi lter insert excludes wetting with sample fl uid during pipetting enabling contamination-free pipetting of DNA or RNA, toxic or infectious samples. piston pipette shaft Filter serving as an aerosol barrier The fi lter insert in the pipette tips serves as an aerosol barrier protecting the pipette against air contamination likely to occur during pipetting and preventing cross talk with subsequent pipetting cycles performed with a new tip and sample. Filter tips certifi ed in accordance with the Biosphere quality and purity standard are available in a range from 0.1 μl to 1,250 μl. sealing surface of pipette tip aerosol aerosol-free filter * Refer to our Application Report Shielding Effect of Filter Inserts in Pipette Tips for additional details sample Reliable safety with selected fi lter materials The fi lter element integrated in the pipette tips consists of a porous plastic material featuring defi ned hydrophobic properties and no self-sealing additives. The sponge-like material is densely composed of randomly arranged channels (see picture on the left), enabling air to pass for precision when using air displacement pipettes. Simultaneously, the complex structure of the porous fi lter safely prevents aerosols from entering the pipette shaft. Added protection with Biosphere purity quality Maximum biological purity thanks to the special production process: free from DNA, RNase, ATP and pyrogens Batch-specifi c certifi cation is available upon request Convenient and safe packaging of individual tip boxes Each tip box is additionally and aseptically protected in a special sterile bag packaging Easy opening of bag packaging without aid via notch at short bag side (see picture) 11

12 Quality Tips with Filter Filter tips (illustrations 50%) Tray Colour Code Max. Sample / Colour Quality Grade Type E μl, 96/box 480/inner box 1,920/case Type E μl, 96/box 480/inner box 1,920/case μl, 480/bag (loose) 4,800/case Type E long μl, 96/box 480/inner box 1,920/case Type D μl, 96/box 480/inner box 1,920/case μl, 480/bag (loose) 4,800/case NEW Type N μl, 96/box 480/inner box 1,920/case Type J μl, 96/box 480/inner box 1,920/case Type A μl, yellow 96/box 480/inner box 1,920/case μl, yellow 480/bag (loose) 4,800/case Type A μl, yellow 96/box 480/inner box 1,920/case μl, yellow 480/bag (loose) 4,800/case Type C μl, 96/box 480/inner box 1,920/case μl, 480/bag (loose) 4,800/case Type L μl, 96/box 480/inner box 1,920/case Type B ,000 μl, blue 100/box 500/inner box 1,000/case ,000 μl, blue 200/bag (loose) 2,000/case Type M ,250 μl, 96/box 768/case ,250 μl, 384/bag (loose) 3,840/case

13 Quality Tips without Filter Tips without filter (illustrations 50%) Tray Colour Code Max. Sample / Colour Quality Grade Type E ,000/bag, 10,000/case /box, 1,920/case μl, 96/box, 480/inner case, 1,920/case Tip StackPack /tray, 576/StackPack, ,304/case Tip StackPack Starter Kit 1,728 + Tip System Box Type E long μl, 500/bag, 10,000/case /box, 1,920/case Type D μl, 1,000/bag, 10,000/case /box, 1,920/case /box, 480/inner case, 1,920/case Type J Type A Type C Type L Type B ,000/bag, 10,000/case μl, 96/box, 1,920/case /box, 480/inner case, 1,920/case /bag, 10,000/case /box, 480/inner case, 1,920/case μl, Tip StackPack yellow 96/tray, 480/StackPack, ,920/case Tip StackPack Starter Kit ,440 + Tip System Box /bag, 10,000/case /box, 480/inner case, 1,920/case μl, Tip StackPack /tray, 480/StackPack, ,920/case Tip StackPack Starter Kit 1,440 + Tip System Box /bag, 5,000/case μl, 96/box, 1,920/case /box, 480/inner case, 1,920/case /bag, 5,000/case ,000 μl, blue 100/box, 1,000/case /box, 500/inner case, 1,000/case Type M /bag, 3,840/case ,250 μl, /box, 3,840/case /box, 768/case /bag, 2,500/case ml Scale 1: /box, 500/case /bag, 2,500/case ml Scale 1: /box, 500/case /bag, 2,000/case ml Scale 1: /box, 250/case Type H: Tips for Ortho BioVue System 360 μl 250/bag, 5,000/case No.:

14 Compatibility Table - Pipette Tips Biosphere Pipette Tips with fi lter (illustrations 50%) Eppendorf Reference μl μl 2-20 μl 2-20 μl μl μl 100-1,000 μl Eppendorf Pipettes Eppendorf Research μl μl 2-20 μl μl μl μl MC 100-1,000 μl 500-5,000 μl 1-10 ml Eppendorf Research plus μl MC 2-20 μl μl μl μl MC 2.5 μl 10 μl 10 μl μl ,250 μl 1,000 μl 300 μl 200 μl 200 μl 100 μl 20 μl 20 μl 20 μl Quality Pipette Tips without fi lter (illustrations 50%) Eppendorf Reference Eppendorf Research Eppendorf Research plus 10 μl μl μl 20 μl 20 μl 20 μl μl ,250 μl 1,000 μl 300 μl 250 μl ml ml ml = max. pipetting volume 10 = limited pipetting volume = not recommended = not checked MC = Multi-channel pipette 14

15 Eppendorf Pipettes Thermo Finnpipettes Gilson Pipettes Eppendorf Research pro Xplorer Finnpipette Digital / F1 / F2 Finnpipette Focus Finnpipette Novus Bio Control Pipetman / Ultra / Concept 100-1,000 μl 500-5,000 μl 1-10 ml μl μl MC μl μl MC μl μl MC 50-1,000 μl 50-1,200 μl MC 100-5,000 μl μl MC μl F μl F μl μl Univ μl MC 2-20 μl Univ μl MC μl Univ μl Univ μl MC 100-1,000 μl 200-1,000 μl μl μl 1-10 μl μl 100-1,000 μl μl μl MC 100-1,000 μl 100-1,200 μl MC μl MC P2/U μl P10/U μl C8/C μl P20/U μl P100/U μl P200/U μl U μl P1,000/U1, ,000 μl P5,000/U5,000 1,000-5,000 μl P10/U ml Eppendorf Research pro Xplorer Finnpipette Digital / F1 / F2 Finnpipette Focus Finnpipette Novus Bio Control Pipetman / Ultra / Concept Pipette suppliers occasionally rework or carry out technical modifications to the pipette cones of their pipettors so that the information provided in the Compatibility Table constitutes a recommendation only. In case of doubt, free sample tips are available on request to test the optimum fitting and ejection behaviour of our tips when used on your pipettes. 15

16 Compatibility Table - Pipette Tips Biosphere Pipette Tips with fi lter (illustrations 50%) μl Biohit Pipettes mline Mechanical Proline Mechanical eline Electronic μl MC 2-20 μl μl μl μl MC 100-1,000 μl 100-5,000 μl μl μl μl 5-50 μl MC μl μl MC μl MC 100-1,000 μl 100-5,000 μl μl μl 50-1,000 μl 50-1,200 μl MC 100-5,000 μl μl 20 μl 20 μl 10 μl 10 μl 10 μl 2.5 μl μl 100 μl ,250 μl 1,000 μl 300 μl 200 μl 200 μl Quality Pipette Tips without fi lter (illustrations 50%) mline Mechanical Proline Mechanical eline Electronic 10 μl μl 200 μl 20 μl 20 μl 20 μl 10 μl ,250 μl 1,000 μl 300 μl 250 μl ml ml ml = max. pipetting volume 10 = limited pipetting volume = not recommended = not checked MC = Multi-channel pipette 16

17 Brand Pipettes Socorex Manual / Electronic Pipettes Transferpette Digital Transferpette S Transferpette Electronic Calibra Manual Micro 822 C. M. Multi 852 Acura Manual Micro 825 Acura Electro Micro 925 A. 835/ 935e Acura Multi 855/955e μl 2-20 μl 2-20 μl 5-50 μl μl μl μl MC μl 100-1,000 μl μl μl μl MC 2-20 μl μl μl μl MC 100-1,000 μl 1-10 ml μl μl MC 2-20 μl 1-20 μl MC μl 100-1,000 μl μl 1-10 μl 1-10 μl 2-20 μl μl μl 100-1,000 μl 1-10 μl μl μl μl μl 1-10 μl 2-20 μl 5-50 μl μl μl 100-1,000 μl ml 0,5-10 μl MC 5-50 μl MC μl MC Transferpette Digital Transferpette S Transferpette Electronic Calibra Manual Micro 822 C. M. Multi 852 Acura Manual Micro 825 Acura Electro Micro 925 A. 835/ 935e Acura Multi 855/955e Pipette suppliers occasionally rework or carry out technical modifications to the pipette cones of their pipettors so that the information provided in the Compatibility Table constitutes a recommendation only. In case of doubt, free sample tips are available on request to test the optimum fitting and ejection behaviour of our tips when used on your pipettes. 17

18 Pipette Tips and Tip SystemBox The Tip SystemBox: Central Pipetting Station Simple and practical Flexible and cost-effective Empty 96-tip refill trays and bulk tips Economic and environmentally friendly Tip StackPack Suitable for manually racking bulk tips for autoclaving and convenient handling.* Efficient handling of 96-tip trays, reduced packaging and storage space in the Tip SystemBox Tip SystemBox The autoclavable Tip SystemBox is the central work station for all 96-tip Sarstedt trays. Complete with hinged lid and anti-slip base, the Tip SystemBox readily accomodates refill trays from Tip StackPack or empty trays for racking. Empty, colour-coded refill trays are available separately. Re-cycling Information Sarstedt are able to offer a Free Service for the collection of empty tip boxes for re-use or re-cycling. Contact Customer Services for further information. 18

19 Tissue Culture Certifi ed Tissue Culture Products In the field of cell and tissue culture, compliance with strict purity standards is essential to achieving reliability and consistency for in vitro applications. Today, cell and tissue cultures are not only used in basic research but also in applied biotechnology and clinical research. Cell cultures are also increasingly used in toxicity tests, for quality control of chemical and biological products as well as for industrial applications, e.g. manufacturing monoclonal antibodies etc. Sarstedt produces sterile disposables for sophisticated tissue culture research. In particular, this range includes the following products: TC flasks TC dishes 96/24/6 Well plates Cell scrapers Filtration units To meet this growing demand and in accordance with our guiding principle that products coming into contact with cells must not have an adverse effect on the cells, these products are manufactured under stringent purity conditions and subjected to comprehensive quality testing. The fi nal product is then certifi ed non-pyrogenic and non-cytotoxic and marked with the Come Grow With Us quality symbol. All tissue culture products meet the following requirements: Sterile In accordance with ISO Sterilisation of health care products-radiation Non-pyrogenic Based on the LAL Test in accordance with the FDA Guidelines on Medical Devices Non-cytotoxic In accordance with EN ISO Biological Evaluation of Medical Devices - Part 5, Test on in-vitro Cytotoxicity 19

20 Tissue Culture Flasks with a Quick-Release Cap Closed Maximum 1/3 turn for ergonomic opening/closing of the fl ask Vented position Lock the cap in the defi ned vented position by a simple turn Open Remove the cap by a quick 1/8 turn from the vented position with a colour code system that offers maximum fl exibility Depending on the cell s affinity to the growth surface and the user s requirements, three different colour coded surface options are available Adherent cells are easily cultivated in tissue culture fl asks with a traditional tissue culture surface that are coded with a red or black quick-release cap. Primary, established epithelial, and other fastidious cell lines that are diffi cult to grow on traditional surfaces may grow better on the growth surface offered by Cell + coded with a yellow quick-release cap. A special coating of the growth surfaces with polylysines, collagens, or gelatins is often not necessary. Special fl asks with a hydrophobic surface that minimises cell loss attributed to unwanted cell attachment are coded with a green quick-release cap. Problem-free cultivation of suspension and hybridoma cultures thanks to the hydrophobic growth surface. Quick detachment of cells from the surface can be achieved by gently tapping the fl asks. 20

21 Cell + Tissue Culture Products Tissue culture products for adherent cells Cell + growth surface for sensitive cell cultures Cell + TC products for the growth of primary cultures and other fastidious cell lines are gamma-sterilised and made from high-quality polystyrene. Cell + TC products particularly support the growth of various fastidious cell lines: Primary cultures Continuous cell lines Cells that normally require a coating of collagen, gelatin or polylysine for growth Cell lines that grow poorly on standard surfaces Cell + TC fl asks with PE quick-release cap PE ventilation cap Yellow cap with and without ventilation - Plug Seal Cap Two-position screw cap with locking mechanism Anti-tip skirt at canted neck Growth Area cm 2 ml Neck Shape bag/300 case w. ventilation bag/300 case bag/100 case w. ventilation bag/100 case Non-wettable 0.2 μm hydrophobic fi lter membrane for reproducible gas exchange bag/40 case w. ventilation bag/40 case Canted neck options with pouring channel Cell + TC dishes in SUREGrip Design Convenient handling due to a raised ring around the circumference of the dish base, enabling an easy and secure grip of both the dish and lid, even from a whole stack. Ø/Height mm Growth Area cm 2 Surface/ Colour Code /10 8 Cell + 20 bag/500 case /15 21 Cell + 20 bag/500 case /20 58 Cell + 20 bag/300 case Cell + TC plates with lid Lid with condensation rings Wells with raised rims Lid can only be placed onto the plate in one direction Stackable Growth Area cm 2 Max. per Well of Wells Surface/ Base Colour Shape Code ml 96 Cell + 1 bag/50 case ml 24 Cell + 1 bag/50 case ml 6 Cell + 1 bag/50 case

22 Tissue Culture Products Tissue culture products for adherent cells PE ventilation cap Non-wettable 0.2 μm hydrophobic fi lter membrane for reproducible gas exchange Tissue culture fl asks with quick-release screw cap Two-position screw cap with positive venting mechanism With or without ventilation cap Made from high-quality polystyrene Sterilised by gamma irradiation Hydrophilic surface for optimal attachment and growth of adherent cells Anti-tip skirt at canted neck TC fl asks with PE quick-release cap Red cap with or without ventilation Plug Seal Cap Two-position screw cap with locking mechanism Anti-tip skirt at canted neck Growth Area cm 2 ml Surface Neck Shape standard 20 bag/300 case w. ventilation standard 20 bag/300 case standard 5 bag/100 case w. ventilation standard 5 bag/100 case standard 5 bag/100 case w. ventilation standard 5 bag/100 case standard 5 bag/40 case w. ventilation standard 5 bag/40 case Canted neck options with pouring channel TC fl asks with black PP cap - Rim Seal Cap Growth Area cm 2 ml Surface Neck Shape standard 20 bag/300 case standard 5 bag/100 case standard 5 bag/100 case standard 5 bag/40 case Canted neck options with pouring channel PE = polyethylene, PP = polypropylene 22

23 Tissue Culture Products Tissue culture products for adherent cells TC plates with lid Lid with condensation rings Wells with raised rims Lid can only be placed onto the plate in one direction Stackable Growth Area cm 2 of Wells Max. Base Shape Surface/ Colour Code ml standard 1 bag/ 50 case ml standard 1 bag/ 50 case ml standard 1 bag/ 50 case ml standard 1 bag/ 50 case TC dishes in SUREGrip Design Convenient handling due to a raised ring around the circumference of the dish base, enabling an easy and secure grip of both the dish and lid, even from a whole stack. Ø/ Height mm Growth Area cm 2 Surface/ Colour Code /10 8 standard 20 bag/500 case /10 8 with grid 20 bag/500 case /15 21 standard 20 bag/500 case /15 21 with grid 20 bag/500 case /20 58 standard 20 bag/300 case non SUREGrip 150/ standard 10 bag/100 case

24 Tissue Culture Products Tissue culture products for suspension and hybridoma cells For minimisation of interactions between cell culture and growth area due to hydrophobic surface Reduced micro adhesion (cell loss) Sterilised by gamma irradiation Anti-tip skirt at canted neck TC fl asks with PE quick-release cap Green cap with or without ventilation - Plug Seal Cap Two position screw cap with locking mechanism Anti-tip skirt at canted neck Growth Area cm 2 ml Neck Shape w. ventilation bag/300 case PE ventilation cap w. ventilation bag/100 case w. ventilation bag/40 case Canted neck options with pouring channel Non-wettable 0.2 μm hydrophobic fi lter membrane for reproducible gas exchange TC dishes in SUREGrip Design Convenient handling due to a raised ring around the circumference of the dish base, enabling an easy and secure grip of both the dish and lid, even from a whole stack. Ø/ Height mm Surface/ Colour Code /10 hydrophobic 20 bag/500 case /15 hydrophobic 20 bag/500 case /20 hydrophobic 20 bag/300 case TC plates with lid, sterile of Wells Max. per Well ml Surface/ Colour Code hydrophobic Base Shape 1 bag/50 case ml ml ml ml hydrophobic hydrophobic hydrophobic hydrophobic 1 bag/50 case bag/50 case bag/50 case bag/50 case

25 Tissue Culture Products Tissue culture products for adherent cells Coverslips for microscopy Highly transparent coverslips for culturing adherent cells Sterilised by gamma irradiation Ø mm Surface/ Colour Code standard 25 bag/200 case standard 25 bag/200 case standard 25 bag/200 case Cell scraper with soft, fl exible blade Pos. 1 An alternative to enzymatic detachment of intact monolayer cultures to avoid cell alterations Easy and more complete separation of monolayer due to extra thin and fl exible blade When downward pressure is applied to the handle, the fl exible blade can remove all cells in its path with one smear Sterilised by gamma irradiation, individually wrapped, sterile Cell scrapers with two-position blade Cell Scraper Length cm Blade mm bag/100 case bag/100 case Pos. 2 turned by 90 Cell scraper for use in larger TC fl asks (T-175) Cell Scraper Length cm Blade mm bag/100 case

26 Filtropur V Vacuum Filtration Sterile vacuum fi ltration units and sterile bottle top fi lters Time- and cost-saving solutions thanks to: Polyether sulfone (PES) membrane filter: High-fl ow membrane for high throughput rates and low protein binding Filtropur V 50 and BT 50 with 0.1 μm PES membrane for effi cient prevention/removal of mycoplasms from solutions Ergonomically shaped receiver bottles 250 ml 1 litre with increased extra volume and improved stability Easy product identification by detailing the fi lter material, pore size and Lot number on the fi lter housing Ideal for cold sterilisation of, e.g. - tissue culture media - protein solutions Filtration units Description Filtration Ø Membrane Membrane/ Pore Size Filtropur V 25 vacuum filter Filtropur V 25 vacuum filter Filtropur V 50 vacuum filter Filtropur V 50 vacuum filter 250 ml 60 mm PES/0.45 μm 1/case ml 60 mm PES/0.22 μm 1/case ml 90 mm PES/0.45 μm 1/case ml 90 mm PES/0.22 μm 1/case 5.94 A sterile screw cap for the collection vessel is included with each fi ltration unit. Bottle top fi lters for storage vessels, 45 mm max. outer diameter Description Filtropur BT 25 bottle top Filtropur BT 25 bottle top Filtropur BT 50 bottle top Filtropur BT 50 bottle top Filtration Ø Membrane Membrane/ Pore Size 250 ml 60 mm PES/0.45 μm 1/case ml 60 mm PES/0.22 μm 1/case ml 90 mm PES/0.45 μm 1/case ml 90 mm PES/0.22 μm 1/case

27 Filtropur S/Filtropur L Filtration Units Sterile Filtropur S syringe and Filtropur L pressure fi ltration systems for fi ltration of aqueous solutions Pre-fi lter free from binding agents and cellulose acetate membrane free from wetting agents to ensure bio-compatibility PES membrane for low protein binding and high throughput rates Individually wrapped and sterile Non-pyrogenic Filtropur S 0.45, yellow housing, for sterilisation and particle removal from solutions Inlet/Outlet Connection Inlet Luer-lock, female outlet Luer-lock, male Filtration Surface Membrane Pore Size 5.3 cm 2 PES membrane 0.45 μm 1 bag/50 case Filtropur S 0.2, dark blue/ housing, for sterile fi ltration Inlet/Outlet Connection Inlet Luer-lock, female outlet Luer-lock, male Filtration Surface Membrane Pore Size 5.3 cm 2 PES membrane 0.2 μm 1 bag/50 case Filtropur S plus 0.2, blue/opaque housing with integral glass-fi bre prefi lter, for sterile fi ltration of viscous solutions (e.g. serum) Inlet/Outlet Connection Inlet Luer-lock, female outlet Luer-lock, male Filtration Surface Membrane Pore Size 5.3 cm 2 CA/GF membrane 0.2 μm 1 bag/50 case Filtropur L pressure fi ltration system with integral prefi lter for sterile fi ltration of media, sera, buffers and other aqueous solutions Filtration range 100 ml to 10 L Individually wrapped, sterile Filtropur L 0.2 S with integral glass-fi bre prefi lter for use in connection with pressure pumps (e.g. membrane pumps) or peristaltic pumps (e.g. tube pumps) Inlet Tube connector (6-12 mm stepped connector) Membrane Pore Size CA/GF membrane 0.2 μm 1 bag/10 case Filtropur L 0.2 LS with integral glass-fi bre prefi lter for use in connection with pressure pumps (e.g. membrane pumps) or peristaltic pumps (e.g. tube pumps) Inlet Luer-Lock Membrane Pore Size CA/GF membrane 0.2 μm 1 bag/10 case

28 CryoPure Storage System CryoPure - Certifi ed Quality CryoPure tubes are subjected to extensive testing to ensure that potential contaminants which could harm cell materials and cellular components during vital preservation are not present. Upon successfully meeting stringent testing criteria, CryoPure tubes are certifi ed as follows: Non-pyrogenic Sterile Non-mutagenic CE/IVD-compliant Non-cytotoxic Exceptional versatility Available in a volume range of 1.2 to 5 ml, highly transparent CryoPure tubes are engineered with a safe, externally threaded screw cap to minimise contamination For more compact storage (10 x 10 compartments), a 2.0 ml CryoPure tube with an internally threaded cap and silicone 0-ring is available Colour-coding options 5 Cap colours and 5 additional cap inserts provide up to 25 different colour-coding options NEW Blue caps and coding inserts for 2 ml CryoPure tubes Ergonomic design QuickSeal screw caps ensure safe, ergonomic opening and closing of both cap types with a single turn CryoRack 40/Work rack Accommodates 40 tubes (4 rows of 10 grooves) Interlocking connection with tube bases provides easy single-handed operation Alphanumeric colour-coding for easy sample identifi cation Anti-slip rubber pads Autoclavable, 121 C, 20 min. CryoRack 40 No (1/bag, 10/case) Cryo boxes for low temperature range storage High-quality durable polycarbonate storage boxes for a deep-freeze temperature range to -196 C Numerical coding at each aperture enables quick sample identifi cation Compatible tube designs ml ml Format 5 x 5 9 x 9 10 x 10 9 x 9 Storage capacity Box size (WxDxH) in mm 75 x 75 x x 132 x x 132 x x 132 x 95 Ideal for tubes with Internal and external thread Internal thread Internal and external thread Colour 5/bag 20/case red yellow blue

29 CryoPure Storage System CryoPure 1.2 ml tubes with external thread Screw Cap Nominal white 1.0 ml red 1.0 ml yellow 1.0 ml 50/bag green 1.0 ml 500/case violet 1.0 ml Colour mix 1.0 ml CryoPure 2.0 ml tubes with external thread Screw Cap Nominal white 1.8 ml red 1.8 ml yellow 1.8 ml green 1.8 ml 50/bag 500/case blue 1.8 ml violet 1.8 ml Colour mix 1.8 ml CryoPure 5.0 ml tubes with external thread Screw Cap Nominal white 4.5 ml red 4.5 ml yellow 4.5 ml 25/bag green 4.5 ml 250/case violet 4.5 ml Colour mix 4.5 ml CryoPure 2.0 ml tubes with internal thread and silicone O-ring Screw Cap Nominal white 1.6 ml red 1.6 ml yellow 1.6 ml green 1.6 ml 50/bag 500/case blue 1.6 ml violet 1.6 ml Colour mix 1.6 ml

30 Storage Boxes Transfer Pipettes PP Storage boxes for sample storage to -90 C High-quality polypropylene storage boxes Numerical coding for each tube in the box enables quick sample identifi cation Range of box options for tubes of 12 mm max. Ø Autoclavable, 121 C, 20 min. Accommodates tubes of max. 45 x 12 mm (e.g , , etc.) Accommodates tubes of max. 45 x 12 mm Colour Lid shape Format/ storage capacity Box size (W x D x H) mm Slip-on lid 9 x 9/ x 130 x 45 5/case Hinged lid 10 x 10/ x 140 x 55 5/case Disposal Box Description Disposal Box for Sharps, Screw Cap, Label Box size (W x H) mm 2 Litre 115 x /case Transfer pipettes, economy Length mm Bulb Drop Size Graduation pcs./case ,5 ml μl 3:0.50 5,000 case ml μl 1:0.25 5,000 case Transfer pipette, sterile Length mm Bulb Drop Size Graduation pcs./case ml μl 1:0.25 1,000 case Transfer pipettes, individually wrapped and sterile Length mm Bulb Drop Size Graduation pcs./case ml μl 3: case ml μl 1: case

31 Micro Tubes Multiply PCR Reaction Tubes Type D Micro tube 1.5 ml, Type D, without skirted base, cap Description pcs./case Screw cap assembled, sterile 1, SafeSeal micro tube, 2.0 ml, PP, with wide hinge Length/Ø mm Colour Pcs./case / /bag 2, Multiply PCR Single Tubes PCR Performance Tested Description ml ml 500 pcs./bag 2,000 pcs./case 500 pcs./bag 2,000 pcs./case ml PCR strip of 8 tubes/multiply -μstrip without lid PCR Performance Tested Description ml Strip of 8 lids for and strips/bag 480 strips/case 120 strips/bag 480 strips/case ml PCR strip of 8 tubes/multiply -μstrippro with attached lid PCR Performance Tested Description ml 120 strips/bag 480 strips/case RackSystem storage and pipetting station for 0.2 ml Multiply PCR tubes and pre-loaded PCR plates Flexible, two-piece system comprising a work tray and support base Convenient 96 well microtiter format to fi t any processing system Stackable design provides space-saving specimen storage Autoclavable at 121 C Description Description PCR work tray, for 96 tubes 5 pcs./case pcs./case red 5 pcs./case Support base for PCR work tray blue 5 pcs./case green 5 pcs./case yellow 5 pcs./case

32 Now Available... miniperm - Bioreactor for protein production miniperm is an easy-to-handle bioreactor for the cultivation of cells in high densities and simultaneous production of cell products, e.g. monoclonal antibodies. Depending on the cell line, cell densities of more than 10 7 cells/ml and product concentrations of several mg/ml can be obtained. High cell densities High product concentrations Easy handling Multiple harvesting Range of cell culture volumes Applications Hybridoma cells Transfected cells Biomass production Virus-producing cells Entomological cells Plant cells nutrient module production module cell product Glucose Lactate NH4 + CO 2 Cell O 2 silicone membrane dialysis membrane Function The miniperm bioreactor is a membrane-based two-compartment system. A dialysis membrane subdivides the culturing chamber of the miniperm bioreactor into a production module (optionally 5, 35 or 50 ml cell culture volume) and nutrient module (400 ml medium volume). The small pore size of the dialysis membrane (MWCO 12.5 kd) prevents both the cells and released cell products from passing through the membrane. These are accumulated to high cell densities and product concentrations in the production module. Simultaneously, the metabolites released from the cells diffuse through the dialysis membrane from the production module into the nutrient module to be diluted or ised in the medium. The outer side of the production module consists of a thin O 2 and CO 2 permeable silicone membrane for optimal gas exchange. 32

33 Now Available... x-well Slide-based tissue culture chambers The x-well cell culture system includes slide-based cell culture vessels for high-resolution microscopy. Slides made from a variety of materials, PCA, glass and coverglass (170μm), in combination with a polystyrene frame are shaped as one- or multi-chamber vessels with different growth surfaces. The product range includes options in a fl ask or 1, 2, 4, or 8 well format. Range of surfaces and formats Excellent microscopic properties Excludes cell transfer from large cell culture vessels to slides Reduced consumption of media and reagents Leaves no adhesive residues on the PCA slide lumox dish Film-based cell culture system The lumox dish stands out for an ultra-thin (25 μm), gas-permeable lumox fi lm base. As a result of the special fi lm base design, lumox dish shows a very low autofl uorescence and a high transparency for exceptionally good microscopy behaviour. The gas-permeable quality of the lumox fi lm base provides a range of advantages. Cells grow directly at the border between the gaseous and liquid phase where the culture medium cannot function as a diffusion barrier. Low autofl uorescence and high transparency Gas-permeable fi lm base Cultivation of adherent cells or suspension cells Available in a diameter of 50 and 35 mm fl exiperm Reusable tissue culture inserts fl exiperm is a reusable silicone insert to subdivide slides or tissue culture dishes (Ø 50 mm), e.g. lumox dish 50, into smaller cultivation units. The highly adhesive fl exiperm bottom adheres to all plain surfaces such as glass, foil and plastic material. Tissue culture chamber from silicone Highly adhesive Suited for DIN slides and tissue culture dishes Autoclavable quadriperm Tissue culture dish for parallel analyses In the four compartments of identical size, the cells can be cultivated either directly in the dish (suspension cells) or on coated or non-coated DIN slides (adherent cells) under identical conditions and in a parallel procedure. In combination with fl exiperm slide and fl exiperm micro 12, the individual compartments can be subdivided into smaller cultivation units. Cell cultivation, fi xation and coloration Easy to handle Swift media exchange Suitable for DIN slides 33

34 Serological Pipettes Serological pipettes from clear polystyrene Individually wrapped in paper/plastic peel packaging Non-pyrogenic, individually wrapped, sterile Easy identifi cation by international colour code Larger pipetting volume due to negative graduation Variable mode of handling due to reverse graduation Optimised mouthpieces for universal fi t in most common pipettors Easy to open, static-free packaging Serological pipettes 1 ml, 2 ml, 5 ml, 10 ml, 25 ml, 50 ml Total / Graduation Description Colour Code pcs./case * 1 ml 1/100 ml plugged, ind. wrapped, sterile yellow 1,000 case * 2 ml 1/100 ml plugged, ind. wrapped, sterile green 1,000 case * 5 ml 1/10 ml plugged, ind. wrapped, sterile blue 500 case * 10 ml 1/10 ml plugged, ind. wrapped, sterile orange 500 case * 25 ml 2/10 ml plugged, ind. wrapped, sterile red 200 case * 50 ml 1/2 ml plugged, ind. wrapped, sterile violet 100 case *non-pyrogenic Aspiration pipette, polystyrene For aspirating media with a vacuum pump Individually wrapped in paper/plastic peel packaging Certifi ed non-pyrogenic Without graduation, without cotton plug Total / Graduation 2 ml/ without graduation Description without plug, without print, ind. wrapped, sterile pcs./case 1,000 case Easy volume identifi cation by international colour code on individual wrapping guide ribs at mouthpiece Silicone adapter gap adapter housing 25 ml Serological pipette in optimised design Due to the weight of 25 ml pipettes, they are apt to have considerable range of movement in pipetting aid adapters. This undesirable motion makes the pipettes inconvenient to handle, impedes precise sampling and may even cause leaks at the pipette/adapter connection. Small guide ribs at the mouthpiece provide an improved fi t in the adapter housing of any pipette aid to minimise undesirable swaying motion of fi lled pipettes Minimises the risk of dripping by a secure grip of the pipette as soon as the small guide ribs on the mouthpiece come into contact with the adapter housing (see illustration) 34

35 Containers Petri dishes Inoculation Spreader Containers with assembled screw cap from HD-PE/PP Tubes (polycarbonate) and lid (polypropylene) autoclavable* at 121 C Material ml Length/Ø mm Description pcs./case PP /70 Neutral cap, HD-PE, graduation up to 200 ml 210 case PP 70 55/44 Yellow cap, HD-PE 500 case PP 70 55/44 Yellow cap, HD-PE, with label 500 case Container with spoon Length/Ø mm Tube Material Description pcs./case /20 PP non-sterile, with label 500 case Petri dishes for bacteriology Made from clear polystyrene, heat resistant up to 80 C Ø/ Height mm Ventilation Cams /20 gamma-sterile with 10 bag/100 case /15 gamma-sterile with 20 bag/200 case /16 with 20 bag/480 case /16 gamma-sterile with 20 bag/480 case Petri dish, square L x W x H mm Ventilation Cams x 100 x 20 gamma-sterile without 4 bag/160 case Gamma-sterile inoculation spreader from polystyrene Time-saving for laboratories with high work loads Easy to use Safe no pathogenic aerosol and cross contamination Description Colour inoculation spreader blue 4/peel pack 500/case

36 Fig. 1: Fluorescence measurement of lumox film and polystyrene bases at 330 nm lumox film Polystyrene base Emission filter (nm) Fig. 2: Light transmission measurement. Detection of low signals, especially at wavelengths of 200 to 300 nm. Wavelength (nm) lumox film Polystyrene base Glass base You may also be interested in the following subjects... Microbiology Sampling, processing and cultivation PCR & Molecular Biology Certified High-Quality Products for PCR, Molecular Biology and Research Cell and Tissue Culture Cell Cultivation, Cryogenic Storage, Filtration, Liquid Handling sterile non-pyrogenic DNA-free RNase-free ATP-free Liquid Handling 1 No.: No.: No.: Transport and Mailing Systems For transportation of diagnostic samples and blood products Micro Tubes lumox dish Gas-permeable tissue culture dish Low autofluorescence High transparency Ideal for microscopy Gas-permeable film base Optimal growth The complete product range for mailing and transport requirements Safe and compliant with transportation guidelines Low protein binding Minimal protein loss Large writing space on frosted, flat cap The lumox cell culture dish stands out for its ultra-thin (25 μm), gas-permeable film base. As a result of the special film base design, lumox products show a very low autofluorescence and a high transparency for exceptionally good microscopy behaviour. Optical properties The low autofluorescence of the lumox film and the good light transmission enable a consistently high sensitivity for assays, imaging and reader techniques. Low autofluorescence and high light transmission Compared to standard polystyrene bases, the lumox film base shows a lower autofluorescence (Fig. 1) and has a higher light transmission (Fig. 2) than polystyrene or glass bases This document may contain information on products that may not be available in particular countries Technical modifi cations reserved No.: CryoPure Storage System Energizing your Cryopreservation No.: SARSTEDT Ltd. 68 Boston Road Leicester LE4 1AW Tel.: Fax: info@sarstedt.co.uk Efficient and reliable cryopreservation SafeSeal Optimal sealing properties Standard micro tubes The all-around solution No.: Screw cap micro tubes For transport, storage and sample preparation No.: Reliable quality and versatility relative fluorescence (RFU) No.: Forensic Swab in a transport tube with ventilation membrane Transmission (%) Maximum purity for optimal safety in preanalytics The new Forensic Swabs from Sarstedt are suited for the collection of both reference samples and evidence of DNA traces at crime scenes. Apart from Forensic Swabs with polystyrene stem, our range also includes a wooden stem option. The Forensic Swab L has an extended handle for the hygienic collection of reference samples. The Forensic Swab XL with extended stem is ideal for the collection of evidence in sexual assault cases. The ventilation membrane in the tube base enables self-drying of the swab within the tube. Drying of the swab in the open, i.e. outside the tube, as in standard systems is unnecessary, thereby excluding the risk of mix-up and contamination. Stringent purity conditions in the production process and a special EtO sterilisation method developed by Sarstedt to comply with the requirements of forensic analysis ensure the absence of DNA contamination. DNA-free EtO sterilised Self-drying within the tube Identification code* for correct matching Individually wrapped, sterile, or with tamper-proof closure * Global article ID number + serial number (SGTIN)/GS1 Germany. The code is printed on the label both in plain script and in a GS1 DataMatrix format on the tube and swab for a correct match (Art. Nos and only). No.: NEW!