Revolutionising Stem Cell Research. Unique Biaxial Bioreactor Designed for TISSUE ENGINEERING & 3D CELL CULTURE. Regeneration System
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1 Regeneration System Unique Biaxial Bioreactor Designed for TISSUE ENGINEERING & 3D CELL CULTURE Revolutionising Stem Cell Research A Member of Quintech Life Sciences
2 TisXell A Renaissance in Regenerative and Translational Medicine QuinXell s Novel TisXell Regeneration System is a 3D culture bioreactor which offers a favourable environment for high growth and high proliferation of cell cultures compared to conventional methods. The biaxial bioreactor controlled environment provides optimal nutrients and gases to growing cells and triggers cellular mechano-transduction signaling pathways to stimulate tissue remodeling onto three-dimensional (3D) scaffolds. Our integrated solution addresses a broad range of tissue engineering processes and cell culture applications including that of single cells on microcarriers and slow growing cells types with unsurpassed cell quality. Unique Features Patented biaxial revolution on two independent orthogonal axes (spin and tumble simultaneously) Flexible operational modes available - biaxial, uniaxial, swing modes Efficient fluid transport within the 3D scaffolds, allowing optimal nutrients and waste exchange to and from the cells, penetrating the deepest core of the scaffolds Spherical design of vessel creates a biaxial flow regime that reduces drag and promotes uniform fluid mixing Key Benefits Accelerates cell growth, differentiation and cell proliferation, mimicking native extracellular matrix (ECM) Supports homogenous cell culture at the surface and core of the 3D scaffolds Maintains functionality and viability of tissue constructs Reduces formation of necrotic neo-tissue that interferes with subsequent implantation process Promotes integration of implants with surrounding tissue and support the structural integrity in regeneration of tissues and organs WE PROVIDE CUSTOMISABLE SOLUTIONS FOR YOUR CELL CULTURING NEEDS
3 For BONE CELLS Enhanced Cell Proliferation and Viability Increased Osteogenic Properties Conventional TisXell Conventional TisXell Increased necrosis in conventional culture conditions after 28 days 3 months after tissue engineered bone graft Intensified Vascular Ingrowth in Rat Femur Tissue Engineered Bone Graft Control Group (Empty Scaffold) MACROSCOPIC IMAGES Stem Cells Grown on Microcarriers MICRO CT 3D IMAGES References a ZY Zhang et al. (2009) Biomaterials b ZY Zhang et al. (2010) Biomaterials c MP Chhaya et al (2015) Biomaterials d Leferink AM et al. (2015) Frontiers in bioengineering and biotechnology vol 3 Article 169 e Luciani N et al (2016) Acta biometerialia f DC Andersen/ CH Jensen (2016) danishcrm.com g Dr Mark Chong (on going)
4 TisXell Specifications Patented Innovative Design Independently-controlled drive axes for biaxial, single axis and swing modes for arm rotation of 1 to 12 rpm, and chamber rotation of 1 to 35 rpm. Direction of rotation is selectable Continuous perfusion with integrated variable speed peristaltic pump providing flow rates from 3 to 500ml/min Oxygenator column unit to facilitate gaseous exchange Medium reservoir to for easy exchange of media. Larger headplate (optional) available with ports for optional temperature, ph, and dissolved oxygen probes TisXell Regeneration System with Control Panel (right) Heat isolated console for system drivers and controller A. Arm Assembly with Sensors B. Weight-balanced rotary arm for 50ml, 500ml and 1000ml spherical culture chambers Built-in sensors to detect arm angle and positions for fully programmable operations A. Touchscreen Remote Panel B. Medium Reservoir
5 Modular Culture Chambers & Accessories Chambers are available in 3 sizes 50ml, 500ml and 1000ml Clear, transparent culture chamber with 360 degrees view With dual containment fluid sealed cap and sterile rotators 50ml(left), 500ml (centre), 1000ml (right) spherical culture chambers are available as standard configurations Accessories include various scaffold holders and compression units Highly Versatile Operation Modes Selectable biaxial, uniaxial and swing modes of operation Programmable speeds, directions and swing angles including cycletrack feature Intuitive touchscreen remote panel with interactive user interface for complete management of system while unit is inside a laminar flow hood and a CO2 incubator
6 Specifications TisXell Base Unit (Left) and Prep Module (Right) Designed for Usability Detachable lightweight prep module for easy handling Prep module easily detached with a single pull of lever Accessibility of all connections and controls from front of unit Uniquely designed Two-Way Rotators and tubing slots to prevent any entangling Standard 500ml media bottle reservoir for easy replacement during runs
7 TisXell Regeneration System (Dual Perfusion) Two completely separate perfusion systems within one TisXell Regeneration System Designed for cultures of two separate cell types within one bioreactor system Two individually-controlled peristaltic pumps allow for unique flow rates in each perfusion system Completely customisable holders for attaching scaffolds MultiXell-4 System (MX-4) Four individually-controlled 50ml and/or 250ml chambers for running four separate experiments Complete management of all features of each chamber via the touchscreen remote panel Four separate peristaltic pumps for four separate perfusion systems Specially designed temperature-controlled CO2 incubator hood to provide sterile environment for all TisXell work *For Illustrative purposes only
8 Contact Information Main Office QuinXell Technologies Pte Ltd 18 Boon Lay Way # TradeHub 21, Singapore Tel: Fax: Vietnam Quintech Life Sciences (Vietnam) Room T6 Building, Hoang Dao Thuy Street Trung Hoa, Cau Giay District. Hanoi, Vietnam Tel: +84 (4) Fax: +84 (4) Contact: Dr NX Khoa China The Oriental Science & Technology Co Ltd No. 265 Central Bei-Si-Huan Road HaiDian District, Beijing , China Contact: Mr Wang Ding Europe Minden LifeScience GmbH Feldstr. 21, D Minden, Germany Contact: Mr Lothar Grannemann A Member Of Quintech Life Sciences
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