HTS automated patch clamp takes cardiac safety testing to the next level

Transcription

1 Nanion Technologies HTS automated patch clamp takes cardiac safety testing to the next level Dr. Elena Dragicevic Senior Scientist / Sales Manager at Nanion Technologies

2 Agenda Meet Nanion Technologies SyncroPatch 384 PE & cardiac ion channels in cell lines and ips CMs Dynamic patch clamping of ips CMs recorded on Patchliner Optogenetics in HTS automated patch clamp systems (SyncroPatch 384 PE ) Summary

3 Nanion Technologies in brief Founded in 2002 in Munich, Germany >90 highly qualified employees worldwide (20 at PhD level) Own offices in USA, China, Japan, Copenhagen Distributors in 7 countries Launch of first product Port-a-Patch (2003) Patchliner (2006), SyncroPatch 96 (2009) Launch of SyncroPatch 384/768 PE (2013) More than 500 systems installed globally Nanion won most prestigious German award for entrepreneurs (2009) Second place at German Zukunftpreis (2014) Europe Israel Japan honouring people who have put their outstanding scientific accomplishments to good use by creating marketable products

4 Agenda Meet Nanion Technologies SyncroPatch 384 PE & cardiac ion channels in cell lines and ips CMs Dynamic patch clamping of ips CMs recorded on Patchliner Optogenetics in HTS automated patch clamp systems (SyncroPatch 384 PE ) Summary

5 Nanion participation in CiPA Part of the leadership team in the HTS ion channel screening subteam: 5 cardiac ion channels recorded on automated patch clamp devices (Patchliner & SyncroPatch384 PE and others) Participant in the myocyte phase II study: hips cardiomyocytes on impedance/efp system: the CardioExcyte96 CiPA power tools SyncroPatch 384/768PE. HTS leadership team member: Dr. Sonja Stolzle-Feix sonja@nanion.de The Patchliner.. The CardioExcyte 96.

6 PE The SyncroPatch 384/768 PE the 4th generation APC Giga-seal patch clamping 384 or 768 recording channels Up to 20,000/40,000 data points per day Made in Unattended operation 4-6 hours 0,16-0,65 per data point Ultra flexible: Can be applied in ALL phases of drug discovery 1x 4x 8x R: low R: medium R: high SyncroPatch 384 PE consumables NPC-384 Variable hole number and size Partial run (any 32 wells per run) Partial plates can be run without using the whole chip

7 PE The SyncroPatch 384/768 PE stable current recordings CHO herg: Overlay of the traces for more than 20 minutes

8 PE The SyncroPatch 384/768 PE temperature control temperature-controlled recordings herg expressed in HEK cells was recorded at RT and 35 C Some like it hot! erythromycin was more potent at 35 C vs. RT as previously reported

9 PE Ion Channels of the CIPA Initiative Na v 1.5 ( INa ) 1 K v 11.1 (herg IKr ) 2 K v 4.3-K Chip 2.2 ( Ito ) 2 Ca v 1.2/b2/a2d ( Ica, L ) 1 K v 7.1-KCNE1 ( IKs ) 2 K ir 2.1 ( IK1 )

10 PE Ion Channels of the CIPA Initiative on ONE plate

11 PE CiPA Step-Ramp vs CiPA Milnes herg protocol Step-Ramp Milnes Raw data traces Current vs. time Raw data traces Current vs. time Neg. Pos. Neg. Pos. Neg. Pos. Neg. Pos. HEK herg cells kindly provided by Chantest

12 PE Pharmacology: Milnes vs. Step-Ramp herg protocol The Milnes and the ramp protocol where compared in terms of IC 50 s of Verapamil and Dofetilide. The IC 50 s were found to be comparable between the two methods. Milnes Step-Ramp HEK herg cells kindly provided by Chantest

13 PE Different (ips CM) cells on one 384-well plate: Cor.4U other cell supplier Cor.4U CHO Nav1.7 The SyncroPatch 384/768 PE the 4th generation APC Made in

14 PE HTS pharmacology on ips CM cells: Cor.4U AP raw trace: CTR vs Nifedipine Online Analysis APD 90 Made in Cor.4U Online Analysis APD90 and APD50

15 Agenda Meet Nanion Technologies SyncroPatch 384 PE & cardiac ion channels in cell lines and ips CMs Dynamic patch clamping of ips CMs recorded on Patchliner Optogenetics in HTS automated patch clamp systems (SyncroPatch 384 PE ) Summary

16 PL Patchliner and it s new features Hardware External & internal exchange Temperature control Voltage & current clamp + dynamic clamp New! Single- & multihole chips, produced in-house Rseries compensation Cooled cell hotel New! Applications Voltage and ligand-gated ion channels Whole-cell & perforated patch clamp Cell lines, primary cells and stem cells Validated for CiPA Minimized cell consumption Easy & customizable analysis tools The Patchliner offers high flexibility and sophistication.

17 PL Minimized cell usage and measurements of ipsc neurons on Patchliner K+ Na+ Cor.4U cells added per well Becker et al., J Pharmacol Toxicol Methods. 2013

18 PL Dynamic clamp in automated patch clamp ipsc-derived CMs pharmacology I K1 was electronically introduced using dynamic clamp and was used to replace injected current to achieve a native resting membrane potential (RMP) of approximately -94 mv. An increase in I K1 conductance resulted in a faster repolarization of the AP and a decrease in AP duration Dynamic clamp approach reveals typical pharmacology of calcium channel-manipulating drugs (action potentials elicted using a 1ms depolarazing pulse and 400 ps/pf I k1

19 Agenda Meet Nanion Technologies SyncroPatch 384 PE & cardiac ion channels in cell lines and ips CMs Dynamic patch clamping of ips CMs recorded on Patchliner Optogenetics in HTS automated patch clamp systems (SyncroPatch 384 PE ) Summary

20 PE The SyncroPatch 384/768 PE with Optical Stimulation First Prototype: 96 blue LEDs (470 nm) below the Chip Constant stimulation at selected intensity possible Stimulation at selected frequency (e.g. 1 Hz) possible

21 PE The SyncroPatch 384/768 PE with Optical Stimulation Depolarization Via ChR2: Nav1.5-ChR2 Example Traces Proof of Concept: CHO cells expressing ChR2 and Nav1.5 were used Cells were measured in Current Clamp (CC) - 90 mv were achieved by LFVC (Low Frequency Voltage Clamp) 1 ms Light Pulse at 2Hz (470 nm) was used for stimulation Cells: ChR2 + Nav1.5 in CHO provided by

22 PE The SyncroPatch 384/768 PE with Optical Stimulation Depolarization Via ChR2: Cor4U-ChR2 Example Traces Proof of Concept: Cor4U cells transfected with ChR2 were used Cell were measured in CC Mode - 90 mv were achieved by LFVC 1 ms Light Pulse at 0.5 Hz (470 nm) was used for stimulation Nav1.5 ChR-2 1 ms Light Stimulus Cells: Cor4U + ChR2 provided by

23 Agenda Meet Nanion Technologies SyncroPatch 384 PE & cardiac ion channels in cell lines and ips CMs Dynamic patch clamping of ips CMs recorded on Patchliner Optogenetics in HTS automated patch clamp systems (SyncroPatch 384 PE ) Summary

24 Summary Holistic view on cardiac pharmacology Myocyte II study Impedance / EFP in beating networks Contractility and electrophysiology CiPA Comprehensive safety assays mechanistic electrophysiological understanding Comprehensive safety assays Patch clamp of single cells Cell lines impedance HTS study EFP In Silico reconstruction human ventricular cellular electrophysiology In silico modelling group

25 Summary Holistic view on cardiac pharmacology Impedance / EFP in beating networks Contractility and electrophysiology CiPA Comprehensive safety assays mechanistic electrophysiological understanding Comprehensive safety assays Patch clamp of single cells Cell lines and ips CM? Na+ Cor.4U... and others In Silico reconstruction human ventricular cellular electrophysiology

26 Summary Holistic view on cardiac pharmacology We have established SOPs for APC and CardioExcyte 96 measurements in frame of the CiPA studies APC: Success rate for those targets were between 80 to 90% after applying the QC filters required from the CiPA guidelines Patchliner and SyncroPatch 384PE are equipped with features that are needed for investigating ips CM: Temperature control Low cell consumption, GigaOhm seals Current clamp Dynamic clamp (ongoing project) SyncroPatch 384/768PE. The Patchliner.. The CardioExcyte SOL