Genesis of the BioSolarH 2 Team

Size: px

Start display at page:

Download "Genesis of the BioSolarH 2 Team"

Edwina Townsend
5 years ago
Views:

1 Genesis of the BioSolarH 2 Team AFOSR MURI June 2005 launch Renewable Bio-solar Hydrogen Production from Roust Oxygenic Phototrophs Founding Members BioSolarH 2 Team Charles Dismukes PU photosynthesis/inorganic Edward Stiefel PU enzymology/inorganic Donald Bryant PSU genetics/mol biology cyanos Matthew Posewitz CSM genetics/mol biology algae Eric Hegg MiSU enzymology/inorganic Robert Bidigare UH microbiology/ecology

2 BioSolarH 2 Team 2007 MURI supported team (other support) 10 co-pis 3 senior researchers 10 young postdoctoral coworkers 14 doctoral candidates 2005 MURI Eastern subgroup Western subgroup LC-MS & NMR research staff: J. Eng I. Pelczer 2006 AF Supplement 2007 AF PU Dismukes PSU Bryant PU Stiefel CSM Posewitz UH Bidigare MiSU Hegg PU Austin MoSU Peters AFRL Pachter GIT Spain Ananyev, Shen Kolling(ACS) Vogl ( 08) Cheng (CSC, 08) Smith Drobnini, Beer Brown, Messer Hatlevik Keymer Galajda (NBTC) Boyd, McGlynn, Zadvorny, Ruebush Trohalaki Carrieri McNeely Bennette Skizim (DOE) Guerra (Fulbright) Garcia- Costas Xu Carrieri McNeely Meuser, Nagy Liao (DoD) Lambert Mulder, McGlynn Ranchou- Peyruse

3 What makes the AFOSR BioSolarH 2 MURI format successful? The Goal Microbial Ecology Quantitative Tools & Models Renewable Bio-solar H 2 Production from Robust Oxygenic Phototrophs Photosynthesis Gene Expression & Manipulation Evolution

4 What makes the AFOSR BioSolarH 2 MURI program successful? Motivation

5 The secret to success is communication: triannual reports & teleconferences

6 July 2005, Princeton July 2006, Princeton August 2007, Breckenridge 10 distinguished international advisors -BioSolarH 2 Face-to-Face Annual Meetings

Our MURI team works together Internal Collaborations Nomadic PIs and students eager to wander Posewitz Lab, Jon Meuser, Bryant Lab: Yu Xu, Bidigare Lab: Susan Brown, Dismukes Lab: Kelsey McNeely,

7 Our MURI team works together Internal Collaborations Nomadic PIs and students eager to wander Posewitz Lab, Jon Meuser, Bryant Lab: Yu Xu, Bidigare Lab: Susan Brown, Dismukes Lab: Kelsey McNeely, Damian Carrieri, Peters Lab: Shawn McGlynn 14 publications, 2 in queue, 12 in preparation 4 specialty instruments built for this project 3 LC-MS instruments purchased for the price of one PU central support for MS/NMR metabolomics & proteomics

Educational impact of the BioSolarH 2 MURI 10 young PhD coworkers 14 doctoral

CSM Posewitz UH Bidigare MiSU Hegg PU Austin MoSU Peters GIT Spain T. Brown C.

Nitchke; L. Fogli, P. Wu,, A. Bautista, D. Chien, J. Chou, N. Slater Byrne 08 A.

Berne MURI spawned new or revised courses Stiefel, Freshman Seminar: Elements of

8 Educational impact of the BioSolarH 2 MURI 10 young PhD coworkers 14 doctoral candidates in training PU Dismukes PSU Bryant PU Stiefel Undergraduate Trainees CSM Posewitz UH Bidigare MiSU Hegg PU Austin MoSU Peters GIT Spain T. Brown C. Louie J. Kouch, P. Curtin, Y. Lee, M. Honigberg, A Cortez, A. Ma, S. Kriston, B. Nitchke; L. Fogli, P. Wu,, A. Bautista, D. Chien, J. Chou, N. Slater Byrne 08 A. Chambers W. Stephens C.Muldoon C. Howe K.Swanson J. Fox C. Berne MURI spawned new or revised courses Stiefel, Freshman Seminar: Elements of Life Dismukes, GEO255 Astrobiology (Bioprospecting Lab) Dismukes, ENV525: Production of Renewable Fuels and Energy

9 Leveraged $ Support & Synergistic Collaborations Translational Activities Austin Lab: 6.19% 7.13% 9.98% 4.75e-006% 2.49% 5.77% 2.38% 4.28% Total = $ % 33.3% AFOSR MURI AFOSR core DOD-DURIP NSF: Photosystems DOE-BES: Half-Cell DOE-JGI: Genomes DOE-JGI: Metagenomes DOE-OS+BER+GTL: H2 Networks DOE-GTL: Metabolomics DOE-EERE: H2 Program DOE-Bioenergy Center Bidigare Lab: Bryant Lab: John Golbeck, PSU Dave Ward, MoSU Igor Brown, NASA Samples: O. Lenz, K. Kovacs, J. Appel Dismukes Lab: Rabinowitz, PU Rabitz, PU Gajda Ajlani, CEA Saclay Ben Hankamer U. Queensland Olaf Kruse, U. Bielfeld, GDR Igor Brown, NASA Patricia Brletic W&J Hegg Lab: Jim Fredrickson at PNNL Helen Kreuzer-Martin at PNNL Robert Hausinger MSU C. Peter Wolk MSU Kazem Kashefi MSU Gemma Reguera MSU Claire Vieille MSU Teresa Theil MSU Tim Donohue,UW Madison Daniel Noguera UW Madison Peters Lab: Nikolay Zorin, Pushchino Joan Broderick, MSU Robert Szilagyi, MSU Posewitz Lab: Bonnie Baxter Westminster Arthur Grossman, Stanford Michael Seibert, NREL Maria Ghirardi, NREL

Public Website (new site under construction) Public Announcement for 2007 BioSolarH2 Mid-Term Review 2007 BioSolarH2 Registration Form RENEWABLE BIO-SOLAR

10 Public Website (new site under construction) Public Announcement for 2007 BioSolarH2 Mid-Term Review 2007 BioSolarH2 Registration Form RENEWABLE BIO-SOLAR HYDROGEN PRODUCTION FROM ROBUST OXYGENIC PHOTOTROPHS Project Announcement FA BioSolar Team Milestones July nd BioSolarH2 symposium Feb 1, 2006: BioSolarH2 team expands Dr. Steven Chu visits Princeton Lab July 2005 Goals and Payoff Kick Off Meeting Program and Photos Research Presentations Robert Bidigare - University of Hawaii John Peters - Montana State University Charles Dismukes - Princeton University Pacific Rim Meeting Presentation IGERT Meeting Presentation

2 -generating hydrogenase Biodiesel Ethanol H 2 Algae 20 um Hydrogen, carbohydrate and lipids are made by both algae and cyanobacteria

11 Renewable, Clean, Water-Based Biosolar H 2 Green Algae & Cyanobacteria Yield Hydrogen, Lipids & Sugars Light Reactions PSI and PSII Dark Reactions light 2 H 2 O O 2 chlorophyll water-splitting enzyme 4 e _ 4 H + CO 2 Triglyceride (Oil) Lipid Synthesis Carbohydrate Synthesis H 2 -generating hydrogenase Biodiesel Ethanol H 2 Algae 20 um Hydrogen, carbohydrate and lipids are made by both algae and cyanobacteria Hydrogen can be made in light and dark Algae are good at photo-production of H2 and cyanobacteria are good at dark-production of H2 Cyanobacteria 2 um

Renewable Bio-solar Hydrogen Production from Robust Oxygenic

Z-scheme genes GMOs Oxygenic Photosynthesis HoxE HoxF ORF?

(6934) Cla I (2981) Eco RI (4977) Cla I (7047) diaphorase moiety

platensis 7098 bp Ni-Fe hydrogenase moiety H 2 O O 2 Original New

using for arrayed rapid, microchemostats sensitive, HTP screening

collection of & cyanobacterial field sites for right NAD-linked

hypercarbonate-requiring catalytic mechanism via strain,

and sequence for symbiotic hydrogenases H 2 -exchanging genes

12 Renewable Bio-solar Hydrogen Production from Robust Oxygenic Phototrophs AFOSR-MURI H 2 mutants field screening genome The H 2 Z-scheme genes GMOs Oxygenic Photosynthesis HoxE HoxF ORF? HoxU HoxY HoxH Bam HI (1484) Nco I (1099) Nco I (3375) Nco I (6934) Cla I (2981) Eco RI (4977) Cla I (7047) diaphorase moiety genomic sequence around hox genes in S. platensis 7098 bp Ni-Fe hydrogenase moiety H 2 O O 2 Original New PI Objectives (>2006) Develop Directed new evolution H 2 sensors using for arrayed rapid, microchemostats sensitive, HTP screening & DNA shuffling Isolation/characterization Screen CMMED collection of & cyanobacterial field sites for right NAD-linked phototrophs hydrogenase Hydrogenase Examine hypercarbonate-requiring catalytic mechanism via strain, computational Arthrospira chemistry maxima Bioprospecting Isolate and sequence for symbiotic hydrogenases H 2 -exchanging genes from microbes halophiles Random & specific mutagenesis for higher H 2 production Genetically combining host phototrophs with optimal hydrogenases

HAWAII. Renewable Bio-solar Hydrogen Production from Robust Oxygenic Phototrophs AFOSR MURI Progress Update: January 2007.

HAWAII. Renewable Bio-solar Hydrogen Production from Robust Oxygenic Phototrophs AFOSR MURI Progress Update: January 2007. PRINCETON PENNSTATE HAWAII Renewable Bio-solar Hydrogen Production from Robust Oxygenic Phototrophs AFOSR MURI Progress Update: January 2007 -BioSolarH 2 Team Charles Dismukes PU photosyn. metabolism/chemistry