Limited Indoor Air Quality Assessment
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1 Limited Indoor Quality Assessment Salinas Elementary School 000 Century Drive West Laredo, Webb County, Texas September 5, 20 Terracon Project No R Prepared for: United Independent School District Laredo, Webb County, Texas Prepared by: Terracon Consultants, Inc. Laredo, Texas
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3 TABLE OF CONTENTS PROJECT DESCRIPTION.... Scope of Work....2 Regulatory Overview Standard of Care Reliance... 2 METHODS... 3 RESULTS Visual Evaluation Total Bioaerosol Analysis Thermal Comfort... 9 SUMMARY... RECOMMENDATIONS... 2 REFERENCES... 2 APPENDIX A: APPENDIX B: APPENDIX C: Analytical Reports Photographs Sample Location Drawing
4 Limited Indoor Quality Assessment Salinas Elementary School 000 Century Drive West Laredo, Webb County, Texas PROJECT DESCRIPTION Terracon Project No R September 5, 20 Terracon Consultants, Inc. (Terracon) is pleased to submit the enclosed report for the Limited Indoor Quality (IAQ) Assessment for Mold for the above-referenced site. This IAQ Assessment was performed in accordance with Terracon Proposal No. P9045-R dated August, 20, and was based on information provided by United Independent School District (UISD) regarding potential organic growth that was observed in a few of the classrooms at the site. The purpose of this report is to present the results of the limited IAQ testing performed on August 8, 5, and 24, 20, in specific areas of concern at Salinas Elementary School in Laredo, Texas. In early August, 20, what appeared to be organic growth was identified on the undersides of some of the desks and other furnishings in Classrooms #8 #23. Later, Classroom #2 was added to the list of assessed areas. The impacts did not appear to affect building materials. The cause of the mold proliferation was attributed to elevated humidity in the indoor air and adjustments to the air conditioning system have been implemented to address the problem. The impacted areas were less than 25 contiguous square feet and were cleaned by UISD Maintenance personnel. The rooms were subsequently thoroughly cleaned under the supervision of SERVPRO, a Texas-licensed Mold Remediation Contractor (MRC). Prior to the commencement of remediation activities, as well as during the cleaning process and for a minimum of 48-hours afterwards, high efficiency air particulate (HEPA) filtration units were placed in the classrooms. UISD requested that Terracon perform air quality testing for mold in these areas. The following is a summary of the findings of the limited IAQ assessment including a discussion of the remediation confirmation sampling laboratory reports, copies of which are attached.. Scope of Work This assessment primarily involved a visual inspection with limited non-culturable air sampling inside the above-identified classroom areas. The initial assessment involved a total of 6 rooms (Classrooms #8 - #23), with an additional room (Classroom #2) included in the final clearance sampling event. Terracon also measured indoor temperature and relative humidity levels in the areas that were sampled. In addition, outdoor samples were collected for comparison purposes. A Terracon employee Mr. Joseph A. Lambert, Texas Department of State Health Services (TDSHS) licensed Mold Assessment Consultant (MAC License #9), performed the field measurements and collected the corresponding mold samples on August 8, 5, and 24, 20. Responsive Resourceful Reliable
5 Limited IAQ Assessment for Mold Salinas Elementary School Laredo, Texas September 5, 20 Terracon Project 9045-R.2 Regulatory Overview The TDSHS regulates fungal assessment and remediation activities under the Texas Mold Assessment and Remediation Rules (TMARR). The TMARR requires that fungal assessments be performed by a TDSHS-licensed Mold Assessment Technician (MAT) or Mold Assessment Consultant (MAC) following specified minimum work practices and procedures. Bulk, surface and air samples collected during a fungal assessment must be analyzed by a TDSHS-licensed mold analysis laboratory. Visible fungal growth that exceeds 25 contiguous square feet is considered a regulated quantity and must be remediated by a licensed Mold Remediation Contractor (MRC) following a site specific fungal remediation protocol prepared by a MAC. Since the visible fungal growth at Salinas Elementary School did not exceed 25 contiguous square feet, the condition was considered non-regulated in accordance with the TMARR. The impacted areas were less than 25 contiguous square feet and were cleaned by UISD Maintenance personnel. Although not required under the TMARR, the rooms were subsequently thoroughly cleaned under the supervision of SERVPRO, a Texas-licensed Mold Remediation Contractor (MRC), using both SERVPRO and UISD personnel..3 Standard of Care The assessment was conducted in the subject areas at Salinas Elementary School based on information provided to Terracon by UISD. This investigation was conducted in a manner consistent with the level of care and skill ordinarily exercised by members of the profession currently practicing under similar conditions in the same locale. Terracon did not attempt to identify every potential exposure or hazard present in the subject building. The results, findings, conclusions, and recommendations expressed in this report are based on conditions encountered during Terracon s assessments conducted on August 8, 5, and 24, 20. Many factors such as weather conditions, building occupancy, ventilation patterns, and seasonal variations in fungal concentrations can affect the conditions observed. The information contained in this report should not be relied upon to represent conditions that existed previously or at a later date. Terracon does not warrant the services of regulatory agencies, laboratories, or other third parties supplying information that may have been used in the preparation of this report..4 Reliance The report has been prepared on behalf of and exclusively for use by UISD for specific application related to the assessed area as discussed. No other individual or entity may rely on this report without written permission of Terracon and UISD. Reliance on this report by UISD. and all authorized parties will be subject to the key understandings and limitations stated in the proposal, this report, and Terracon s Agreement for Services. The limitation of liability defined in Terracon s Agreement for Services is the aggregate limit of Terracon s liability to UISD and all relying parties. Responsive Resourceful Reliable 2
6 Limited IAQ Assessment for Mold Salinas Elementary School Laredo, Texas September 5, 20 Terracon Project 9045-R METHODS The visual evaluation was conducted in general accordance with guidelines published by the TDSHS (Texas Mold Assessment and Remediation Rules, 2004), the American Conference of Governmental Industrial Hygienists (Bioaerosols: Assessment and Control, 999), and American Standard Test Methods (Standard Guide for Assessment of Fungal Growth in Buildings, ASTM D338-0). The indoor environment was evaluated for visual evidence of moisture intrusion, visible fungal growth, or other hygiene concerns that could impact indoor air quality. Destructive sampling/testing or disassembly of mechanical components to evaluate hidden, interstitial spaces was not within the scope of work for the project. Terracon collected fungal spore trap samples using AllergencoD sampling cassettes and a Buck automated pump (Buck Bioe Sampling Pump, Model B520), calibrated to a flow rate of 5 liters per minute for 5 minutes per sample. After air sample collection, the sample cassettes were shipped under chain-of-custody (COC) to Mycotech Biological, Inc., a licensed TDSHS laboratory facility. Temperature and relative humidity were measured at representative locations using a EXTECH Model Digital Thermohygrometer (Serial #06503) and/or a AMPROBE Model TH-2A Digital Thermohygrometer (Serial #040382). RESULTS 3. Visual Evaluation General Observations: The subject area consisted of Classrooms #8 - #23 and #2. The majority of the interior walls consisted of painted cinderblock (concrete masonry units - CMU) construction. The ceiling systems consisted of suspended acoustical ceiling tiles. The floors in these classrooms were covered with vinyl floor tile. Evidence of water intrusion and/or fungal growth was not observed during the field visits except as follows: During the initial field visit conducted on August 8, 20, minor apparent mold growth was observed on a cabinet and bathroom door in Rm #9. It was also noted that this room, as well as a number of the other assessed areas, were generally grimy and were in need of considerable cleaning. Water stains were not observed on the ceiling tiles or walls of the assessed areas. During the follow-up field visits conducted on August 5 and 24, 20, it was observed that the assessed classroom areas were notably cleaner than during the initial sampling event. Evidence of visible mold growth was not observed in the classroom areas that were assessed. The building materials appeared to be in good condition and the areas appeared to be clean. Excessive dust was not observed on the supply and return air vents/diffusers. It is Terracon s understanding that SERVPRO, a Texas-licensed MRC, was contracted by UISD to assist and supervise the remediation activities. These activities included a thorough wet-wipe and HEPA vacuum cleaning. Responsive Resourceful Reliable 3
7 Limited IAQ Assessment for Mold Salinas Elementary School Laredo, Texas September 5, 20 Terracon Project 9045-R In addition, it is Terracon s understanding that higher efficiency filters were recently installed in the air handling units that service the areas of concern. 3.2 Total Bioaerosol Analysis An airborne biogenic particle, such as a fungal spore, is a bioaerosol. Fungal spores are ubiquitous in the environment and have specific requirements for survival, germination, and growth. Although fungal bioaerosols have not been conclusively associated with adverse health effects commonly noted in typically building-related complaints (e.g., mucous membrane irritation, headache, and fatigue), they are known in the medical literature to be associated with common allergy or more serious respiratory disease. Some studies and case histories have shown correlations between allergy, or more serious respiratory symptoms, and fungal contamination of heating, ventilating, and air-conditioning (HVAC) systems. The spore trap sampling method used for total bioaerosol sampling is capable of determining total fungal spore concentrations per cubic meter of air (spores/m 3 ). While many fungal spores can be identified by this method, others, which are morphologically similar, cannot be positively assigned a taxa designation and are grouped based on similar spore morphology (e.g., Aspergillus/Penicillium, Drechslera/Bipolaris). In the case of the Salinas Elementary School project, however, two Slide-Lift samples collected from the aforementioned areas in Classroom #9 confirmed that the elevated mold levels were primarily due to Aspergillus proliferation. High variability in fungal spore concentrations will exist in different geographic locations, during different seasons, and weather patterns, and over the course of a given day. As a general rule, indoor fungal spore concentrations in an HVAC-supplied building are typically less than, but qualitatively similar to, fungal spore concentrations found in the outside environment. To assist in the interpretation of bioaerosol sampling results, a comparative sampling strategy is employed. Generally speaking, fungal bioaerosols in the indoor environment should be lower than the contemporaneously collected outdoor control samples. For fungal spores that are unique to the indoor samples (not encountered in the outdoor samples), the results should not exceed more than 50 % of the High results for Typical Outdoor Spore Levels (Texas) as reported in the EMLab P & K IAQ Pocket Reference Guide, 6 th Edition (). Laboratory Initial Assessment Sampling Based on the findings of the sampling conducted during the initial site visit conducted on August 8, 20, elevated levels of mold were identified in nine of the classrooms (Rms #8 #5, #). The indoor mold proliferation was attributed to Aspergillus, a common mold genus. Terracon recommended that the classrooms be thoroughly cleaned by vacuuming with a HEPA vacuum and wet-wiping all accessible surfaces. In an abundance of caution, UISD directed that two additional classrooms (#6 and #8) also be cleaned. As mentioned above, the cleaning was conducted under the supervision of SERVPRO, a Texas-licensed Mold Remediation Contractor IAQ Pocket Reference Guide 6 th Edition, EMLab P & K. Responsive Resourceful Reliable 4
8 Room 9 Room 20 Room 2 Room 22 Room 23 Outdoors (North) Outdoors (South) Limited IAQ Assessment for Mold Salinas Elementary School Laredo, Texas September 5, 20 Terracon Project 9045-R (MRC), using both SERVPRO and UISD personnel. Prior to the commencement of remediation activities, as well as during the cleaning process and for a minimum of 48-hours afterwards, HEPA filtration units (air scrubbers) were placed in the classrooms. The total bioaerosol results for the areas determined to be acceptable from the sampling conducted on August 8, 20 are presented in the laboratory report in Appendix A and below in Graph. GRAPH, TOTAL BIOAEROSOLS (August 8, 20 Sampling Event) 6,000 4,000 3,36 2,000 0,000 8,000 Spores/m 3 6,000 4,000 4,028 2,000, Responsive Resourceful Reliable 5
9 Limited IAQ Assessment for Mold Salinas Elementary School Laredo, Texas September 5, 20 Terracon Project 9045-R Remediation Confirmation Sampling The results of the confirmation sampling conducted on August 5, 20 indicated that of the rooms that were cleaned, all but Classroom #9 had acceptable IAQ levels of mold. Although the levels of mold had dropped considerably from the initial sampling, the Aspergillus/Penicillium result was still elevated in Classroom #9. Terracon recommended that Rm #9 be thoroughly recleaned. As was done previously, HEPA filtration units (air scrubbers) were placed in the classroom prior to the commencement of the recleaning activities, as well as during the cleaning process and for a minimum of 48-hours afterwards. The average indoor total bioaerosol (mold spores and ) results were lower relative to the average outdoor total bioaerosol results on the day of the sample collection. The lower limit of detection (LOD) for the method was 3 particles/m 3 for the indoor samples and 38 particles/m 3 for the outdoor samples, based on a sample volume of 5 liters. The average total bioaerosol concentration in the indoor environment was 49 particles/m 3 (n = ), with a range of 26 to 442 particles/m 3. The total bioaerosol results for one of the classrooms (Rm #9 with 442 particles/m 3 ), was considered elevated and Terracon recommended recleaning/additional HEPA air filtration. The remaining ten rooms that passed the remediation clearance testing had an average total bioaerosol concentration in the indoor environment of 20 particles/m 3 (n = 0), with a range of 26 to 3 particles/m 3. The average total bioaerosol concentration in the outdoor environment was 4,655 particles/m 3 (n = 2), with a range of 4,30 to 4,940 particles/m 3. The total bioaerosol results documented in the laboratory report are based on empirical sample and analysis methods. The total bioaerosol results for the areas determined to be acceptable from the sampling conducted on August 5, 20 are presented in laboratory report in Appendix A and below in Graph 2. Responsive Resourceful Reliable 6
10 Room 8 Room 0 Room 2 Room 3 Room 4 Room 5 Room 6 Room Room 8 Outdoors (North) Outdoors (South) Limited IAQ Assessment for Mold Salinas Elementary School Laredo, Texas September 5, 20 Terracon Project 9045-R GRAPH 2, TOTAL BIOAEROSOLS (August 5, 20 Sampling Event) 6,000 5,000 4,940 4,30 4,000 3,000 Spores/m 3 2,000, Responsive Resourceful Reliable
11 Limited IAQ Assessment for Mold Salinas Elementary School Laredo, Texas September 5, 20 Terracon Project 9045-R Recleaning/Retesting Based on the results of the samples collected on August 5, 20, Rm #9 was thoroughly recleaned. As before, these activities included a thorough wet-wipe and HEPA vacuum cleaning that were conducted under the supervision of SERVPRO. HEPA filtration units were again placed in the classroom prior to the commencement of the recleaning activities, as well as during the cleaning process and for a minimum of 48-hours afterwards. At the direction of UISD, HEPA filtration units were also placed in Rm #, as well as in Rm #2 which reportedly had a small area with apparent mold growth on some paper items found inside a closet. On August 24, 20, Terracon conducted a final round of confirmation sampling in Classrooms #9, # and #2. The average indoor total bioaerosol (mold spores and ) results were lower relative to the average outdoor total bioaerosol results on the day of the sample collection. The lower limit of detection (LOD) for the method was 3 particles/m 3 for both the indoor and outdoor samples, based on a sample volume of 5 liters. The average total bioaerosol concentration in the indoor environment was 52 particles/m 3 (n = 3), with a range of 26 to 9 particles/m 3. The average total bioaerosol concentration in the outdoor environment was 42 particles/m 3 (n = 3), with a range of 32 to 598 particles/m 3. The test results for all of the three rooms indicated that mold was not present at levels exceeding acceptable IAQ levels. The total bioaerosol results documented in the laboratory report are based on empirical sample and analysis methods. The total bioaerosol results for the sampling conducted on August 24, 20 are presented in laboratory report in Appendix A and below in Graph 3. Responsive Resourceful Reliable 8
12 Room 9 Room Room 2 Outdoors (W/NW) Outdoors (West) Outdoors (South) Limited IAQ Assessment for Mold Salinas Elementary School Laredo, Texas September 5, 20 Terracon Project 9045-R GRAPH 3, TOTAL BIOAEROSOLS (August 24, 20 Sampling Event) Spores/m To date, there are no data that support a medically based threshold limit or dose-response relationship for exposure to fungal aerosols or fungal aeroallergens. State or federal exposure limits have not been established for fungal aerosols. 3.3 Thermal Comfort Indoor air temperature and relative humidity are physical conditions important to the perception of comfort. The American Society of Heating, Refrigerating, and -Conditioning Engineers (ASHRAE) have published recommendations regarding thermal comfort. ASHRAE Standard , Thermal Environmental Conditions for Human Occupancy, identifies six primary factors that affect comfort: metabolic rate (affected by the activity being performed), clothing insulation, air temperature, radiant temperature, air speed, and humidity. Responsive Resourceful Reliable 9
13 Limited IAQ Assessment for Mold Salinas Elementary School Laredo, Texas September 5, 20 Terracon Project 9045-R Although the relationships are complex, a temperature range between 68 and 9 degrees Fahrenheit ( F) is recommended for persons working at sedentary to moderately elevated physical activity levels. In order to avoid conditions sufficiently moist to promote the growth of molds or other biogrowth, relative humidity should not exceed 60%. Although the relationships are complex, a temperature range between 3 and 9 degrees Fahrenheit ( F) with relative humidity between roughly 20 and 60 percent (%) are recommended for persons performing office work and wearing light summer clothing. Higher temperatures require lower humidity for comfort. For persons in winter clothing, temperatures can range between 68 F and 5 F, with relative humidity between 30 % and 60 %. Relative humidity should not exceed 60% for extended periods or microbial growth could occur on surfaces that are cool enough for moisture in air to condense. The temperature and relative humidity results for the locations evaluated are presented below in Table 2.0. TABLE 2.0, THERMAL COMFORT Location Temperature Relative Humidity Date 2 Room #8.6 F 64.6% 8/5/20 Room #9 0.3 F 59.3% 8/24/20 Room # F 6.6% 8/5/20 Room # 68. F 62.5% 8/24/20 Room #2 0. F 68.2% 8/5/20 Room #3 0. F 69.% 8/5/20 Room # F 64.5% 8/5/20 Room #5 6.4 F 6.4% 8/5/20 Room #6.0 F 65.3% 8/5/20 Room # 69.2 F 59.5% 8/5/20 Room # F 63.2% 8/5/20 Room #9 2.5 F 50.% 8/8/20 Room # F 5.2% 8/8/20 Room # F 5.8% 8/8/20 Room # F 55.0% 8/8/20 Room # F 62.% 8/8/20 Room #2 0.8 F 6.5% 8/24/20 Temperature and humidity measurements reflect conditions during final clearance sampling event. Responsive Resourceful Reliable 0
14 Limited IAQ Assessment for Mold Salinas Elementary School Laredo, Texas September 5, 20 Terracon Project 9045-R The temperature levels measured during the three field visits were within or below the range between 3 F and 9 F. Many of the relative humidity measurements were at or above the upper end of the recommended range of 20 and 60 percent (%). It is Terracon s understanding that the temperature of the HVAC system was lowered at the request of a number of the teachers and to lower the relative humidity. SUMMARY Terracon was requested to conducted a Limited Indoor Quality (IAQ) Assessment for Mold in Classrooms #8 #23 at Salinas Elementary School. Later, Classroom #2 was added to the list of assessed areas. Based on the findings of the sampling conducted during the initial site visit conducted on August 8, 20, elevated levels of mold were identified in nine of the classrooms (Rms #8 #5, #). The indoor mold proliferation was attributed to Aspergillus, a common mold species, based upon surface sampling results. Terracon recommended that the classrooms be thoroughly cleaned by vacuuming with a HEPA vacuum and wet-wiping all accessible surfaces. At the direction of UISD, two additional classrooms (#6 and #8) were also cleaned. As mentioned above, the cleaning was conducted under the supervision of SERVPRO, a Texaslicensed Mold Remediation Contractor (MRC), using both SERVPRO and UISD personnel. Prior to the commencement of remediation activities, as well as during the cleaning process and for a minimum of 48-hours afterwards, HEPA filtration units (air scrubbers) were placed in the classrooms. During the follow-up field visits conducted on August 5 and 24, 20, it was observed that the assessed classroom areas were notably cleaner than during the initial sampling event. Evidence of visible mold growth was not observed in the classroom areas that were assessed. The building materials appeared to be in good condition and the areas appeared to be clean. The results of the confirmation sampling conducted on August 5, 20 indicated that of the rooms that were cleaned, all but Rm #9 had acceptable IAQ levels of mold. Although the levels of mold had dropped considerably from the initial sampling, the Aspergillus/Penicillium result was still elevated in Rm #9. Terracon recommended that Rm #9 be thoroughly recleaned. As was done previously, HEPA filtration units (air scrubbers) were placed in the classroom prior to the commencement of the recleaning activities, as well as during the cleaning process and for a minimum of 48-hours afterwards. At the direction of UISD, HEPA filtration units were also placed in Rm #, as well as in Rm #2 which reportedly had a small area with apparent mold growth on some paper items found inside a closet. On August 24, 20, Terracon conducted a final round of confirmation sampling in Rm #9. At the direction of UISD, Rm # was also retested, and Rm #2 was added to the list. The test results for all of the three rooms indicated that mold was not present at levels exceeding acceptable IAQ levels. Copies of the laboratory reports are attached. Responsive Resourceful Reliable
15 Limited IAQ Assessment for Mold Salinas Elementary School Laredo, Texas September 5, 20 Terracon Project 9045-R RECOMMENDATIONS Based on the field observations and analytical results of the limited IAQ survey conducted for this project, no additional assessment for mold in the areas that were assessed at Salinas Elementary School is warranted at this time. For improved comfort and overall indoor air quality, additional measures may be necessary to reduce the relative humidity levels in some of the areas at the school. It is also important to maintain clean building conditions for overall healthy indoor air quality. REFERENCES. The Texas Mold Assessment and Remediation Rules, (25 TAC ), Texas Department of State Health Services, May 6, American Conference of Governmental Industrial Hygienists; Bioaerosols: Assessment and Control, Standard Guide for Assessment of Fungal Growth in Buildings, ASTM D Responsive Resourceful Reliable 2
16 APPENDIX A: ANALYTICAL REPORTS
17 August 8, 20 Sampling Event
18 Terracon - San Antonio 69 Blanco Rd. San Antonio, TX 826 TERRACONS69 Sample No: (0) Outside (North) Mycotech Biological, Inc. AIHA EMPAT NO: TDSHS LIC. NO: LAB063 Analysis Type : Media : Sample No: (02) 2 Rm. #8 Allergenco D Hex-Sil Project : Salinas Elementary School Report No: Received date: Report date: Sample No: (03) 3 Rm. # /9/20 8/0/20 Sample Type: Post Sample Date: 8/8/20 Date Analyzed: 8/0/20 % Analyzed: 33% of Trace at 400X Magnification Reporting Limit: 38 Sample Type: Post Sample Date: 8/8/20 Date Analyzed: 8/0/20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Sample Type: Post Sample Date: 8/8/20 Date Analyzed: 8/0/20 % Analyzed: 33% of Trace at 400X Magnification Reporting Limit: ,28, 2 Asper./Pen. - like Asper./Pen. - like 68 26,06 05 Alternaria spp. 38, 2, 3 9, 2 3 4, 2 Ascospores - like 83 3,54, 05 Alternaria spp. 3, 2, 3 38 Basidiospores - like 0 380, 05 Basidiospores - like 3, 05 Nigrospora spp Cercospora spp. 38 Chaetomium spp. 3, 2, 3 3,36 Particles / M 3 02 Particles / M 3 26,258 Particles / M Rocky Creek Rd.. Dripping Springs, Texas Tele:
19 Terracon - San Antonio 69 Blanco Rd. San Antonio, TX 826 TERRACONS69 Sample No: (04) 4 Rm. #0 Mycotech Biological, Inc. AIHA EMPAT NO: TDSHS LIC. NO: LAB063 Analysis Type : Media : Sample No: (05) 5 Rm. # Allergenco D Hex-Sil Project : Salinas Elementary School Report No: Received date: Report date: Sample No: (06) 6 Rm. # /9/20 8/0/20 Sample Type: Post Sample Date: 8/8/20 Date Analyzed: 8/0/20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Sample Type: Post Sample Date: 8/8/20 Date Analyzed: 8/0/20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Sample Type: Post Sample Date: 8/8/20 Date Analyzed: 8/0/20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Asper./Pen. - like Asper./Pen. - like 39,80 05 Asper./Pen. - like , , , 2 Basidiospores - like 3, 05 3 Alternaria spp. 3, 2, Particles / M 3,950 Particles / M Particles / M Rocky Creek Rd.. Dripping Springs, Texas Tele:
20 Terracon - San Antonio 69 Blanco Rd. San Antonio, TX 826 TERRACONS69 Sample No: (0) Rm. #3 Mycotech Biological, Inc. AIHA EMPAT NO: TDSHS LIC. NO: LAB063 Analysis Type : Media : Sample No: (08) 8 Rm. #4 Allergenco D Hex-Sil Project : Salinas Elementary School Report No: Received date: Report date: Sample No: (09) 9 Rm. # /9/20 8/0/20 Sample Type: Post Sample Date: 8/8/20 Date Analyzed: 8/0/20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Sample Type: Post Sample Date: 8/8/20 Date Analyzed: 8/0/20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Sample Type: Post Sample Date: 8/8/20 Date Analyzed: 8/0/20 % Analyzed: 33% of Trace at 400X Magnification Reporting Limit: 38 Asper./Pen. - like 09,4 05 Asper./Pen. - like 88,44 05 Asper./Pen. - like 5 5, , , ,890, 2 Nigrospora spp. 3 Drechslera - like 2 6, 2, 3, 05 48,534 Particles / M 3,326 Particles / M 3 2,22 Particles / M Rocky Creek Rd.. Dripping Springs, Texas Tele:
21 Terracon - San Antonio 69 Blanco Rd. San Antonio, TX 826 TERRACONS69 Sample No: (0) 0 Rm. #6 Mycotech Biological, Inc. AIHA EMPAT NO: TDSHS LIC. NO: LAB063 Analysis Type : Media : Sample No: () Rm. # Allergenco D Hex-Sil Project : Salinas Elementary School Report No: Received date: Report date: Sample No: (2) 2 Rm. # /9/20 8/0/20 Sample Type: Post Sample Date: 8/8/20 Date Analyzed: 8/0/20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Sample Type: Post Sample Date: 8/8/20 Date Analyzed: 8/0/20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Sample Type: Post Sample Date: 8/8/20 Date Analyzed: 8/0/20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Asper./Pen. - like Asper./Pen. - like Asper./Pen. - like , ,08, , 2 Curvularia spp. 3, 2 Curvularia spp. 3, Particles / M 3 4,0 Particles / M Particles / M Rocky Creek Rd.. Dripping Springs, Texas Tele:
22 Terracon - San Antonio 69 Blanco Rd. San Antonio, TX 826 TERRACONS69 Sample No: (3) 3 Rm. #9 Mycotech Biological, Inc. AIHA EMPAT NO: TDSHS LIC. NO: LAB063 Analysis Type : Media : Sample No: (4) 4 Rm. #20 Allergenco D Hex-Sil Project : Salinas Elementary School Report No: Received date: Report date: Sample No: (5) 5 Rm. # /9/20 8/0/20 Sample Type: Post Sample Date: 8/8/20 Date Analyzed: 8/0/20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Sample Type: Post Sample Date: 8/8/20 Date Analyzed: 8/0/20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Sample Type: Post Sample Date: 8/8/20 Date Analyzed: 8/0/20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 6 8, 2 06,38, , Nigrospora spp. 3 8 Particles / M 3,4 Particles / M 3 82 Particles / M Rocky Creek Rd.. Dripping Springs, Texas Tele:
23 Terracon - San Antonio 69 Blanco Rd. San Antonio, TX 826 TERRACONS69 Sample No: (6) 6 Rm. #22 Mycotech Biological, Inc. AIHA EMPAT NO: TDSHS LIC. NO: LAB063 Analysis Type : Media : Sample No: () Rm. #23 Allergenco D Hex-Sil Project : Salinas Elementary School Report No: Received date: Report date: Sample No: (8) 8 Outside (South) /9/20 8/0/20 Sample Type: Post Sample Date: 8/8/20 Date Analyzed: 8/0/20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Sample Type: Post Sample Date: 8/8/20 Date Analyzed: 8/0/20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Sample Type: Post Sample Date: 8/8/20 Date Analyzed: 8/0/20 % Analyzed: 33% of Trace at 400X Magnification Reporting Limit: , 2 3, 2 6 2,38, 2 Alternaria spp. 3, 2, 3 Basidiospores - like 3, 05 Alternaria spp. 5 90, 2, 3 Drechslera - like 3, 2, 3, 05 Ascospores - like 29,02, 05 Basidiospores - like 38, 05 Drechslera - like 2 6, 2, 3, Nigrospora spp. 2 6 Cercospora spp Fusarium spp. 38, 2, 3 65 Particles / M 3 39 Particles / M 3 4,028 Particles / M Rocky Creek Rd.. Dripping Springs, Texas Tele:
24 Terracon - San Antonio 69 Blanco Rd. San Antonio, TX 826 TERRACONS69 Mycotech Biological, Inc. AIHA EMPAT NO: TDSHS LIC. NO: LAB063 Analysis Type : Media : Micro Tape Project : Salinas Elementary School Report No: Received date: 8/9/20 Report date: 8/0/20 Sample No: (9) Rm. #9 (Drawer Near Sink) Sample No: (20) 2 Rm. #9 (Inside Bathroom Door) Sample Type: Post Sample Type: Post Sample Date: 8/8/20 Sample Date: 8/8/20 Direct Direct Date Analyzed: 8/0/20 Date Analyzed: 8/0/20 % Analyzed: Not Applicable % Analyzed: Not Applicable Reporting Limit: N/A Reporting Limit: N/A Aspergillus spp., 2, 3, 29, 2, 29 Aspergillus spp., 2, 3, Rocky Creek Rd.. Dripping Springs, Texas Tele:
25 Project: Salinas Elementary School Report No: Client: Terracon - San Antonio General Comment Reference Page ONLY COMMENT NUMBERS INDICATED ON REPORT ARE RELEVANT. Mycotech Biological is not responsible for any errors resulting from improper or incorrect sampling procedures, atmospheric conditions at the time of sampling or during shipment, or from shipping conditions or methods. relate only to samples analyzed.. This is a known and documented aeroallergen. It may cause an allergic reaction to hypersensitive individuals at normal airborne concentrations. Chronic exposure, at above normal airborne concentrations, may also result in the sensitization and development of allergic disease in previously unaffected individuals. 2. This fungus is an opportunistic pathogen. Many factors affect host contraction; however, this fungus will typically infect only those who are immuno-compromised. Immuno-compromization may be a function of age, sex, race, state of health, or nutrition. Individuals exposed to immunotherapy, chemotherapy, radiotherapy, immunosuppressant drugs, or who have contracted an immunological disorder, are at greater risk of infection. As with other diseases, opportunistic infections may be contracted by a variety of potential routes including injection, ingestion, skin contact and/or respiration. 3. Various species within this Genus/Group have been documented as producing mycotoxins. Mycotoxins represent a wide variety of secondary metabolites produced by fungi that have been documented as toxic to humans. borne mycotoxin concentrations and their specific relationship to human health has not been established to date.. The observed represented desiccated/unorganized hyphal fragments that are not representative of established fungal growth. The presence of this is commonly identified in typical dust and debris collections. Organized are the tubular filamentous parts of a fungus that represents the structural entity of the majority of the fungi. 05. Due to the absence of supporting data, a definitive Genus could not be assigned. 29. The identified organism or organized mycelium revealed characteristics that are consistent with fungal growth. Chris Wardlaw, B.S. PAACB Certified Spore Analyst Laboratory Manager Mycotech Biological, Inc. 8 End of Report
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28 August 5, 20 Sampling Event
29 Terracon - San Antonio 69 Blanco Rd. San Antonio, TX 826 TERRACONS69 Sample No: (0) Outside (North) Mycotech Biological, Inc. AIHA EMPAT NO: TDSHS LIC. NO: LAB063 Analysis Type : Media : Sample No: (02) 2 Rm. 8 Allergenco D Hex-Sil Project : Salinas Elementary School Report No: Received date: Report date: Sample No: (03) 3 Rm /6/20 8//20 Sample Type: Clearance Sample Date: 8/5/20 Date Analyzed: 8//20 % Analyzed: 33% of Trace at 400X Magnification Reporting Limit: 38 Sample Type: Clearance Sample Date: 8/5/20 Date Analyzed: 8//20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Sample Type: Clearance Sample Date: 8/5/20 Date Analyzed: 8//20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: ,432, 2 Cladosporium sp. 6 8, 2 Asper./Pen. - like Alternaria spp. 3 4, 2, 3 3 3, 2 Ascospores - like , 05 Nigrospora spp Basidiospores - like 3 494, 05 Drechslera - like 38, 2, 3, 05 Curvularia spp. 38, ,30 Particles / M 3 04 Particles / M Particles / M Rocky Creek Rd.. Dripping Springs, Texas Tele:
30 Terracon - San Antonio 69 Blanco Rd. San Antonio, TX 826 TERRACONS69 Sample No: (04) 4 Rm. 0 Mycotech Biological, Inc. AIHA EMPAT NO: TDSHS LIC. NO: LAB063 Analysis Type : Media : Sample No: (05) 5 Rm. Allergenco D Hex-Sil Project : Salinas Elementary School Report No: Received date: Report date: Sample No: (06) 6 Rm /6/20 8//20 Sample Type: Clearance Sample Date: 8/5/20 Date Analyzed: 8//20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Sample Type: Clearance Sample Date: 8/5/20 Date Analyzed: 8//20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Sample Type: Clearance Sample Date: 8/5/20 Date Analyzed: 8//20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Drechslera - like 3, 2, 3, 05 Asper./Pen. - like , 2 Curvularia spp. 3, , Particles / M 3 43 Particles / M 3 04 Particles / M Rocky Creek Rd.. Dripping Springs, Texas Tele:
31 Terracon - San Antonio 69 Blanco Rd. San Antonio, TX 826 TERRACONS69 Sample No: (0) Rm. 3 Mycotech Biological, Inc. AIHA EMPAT NO: TDSHS LIC. NO: LAB063 Analysis Type : Media : Sample No: (08) 8 Rm. 4 Allergenco D Hex-Sil Project : Salinas Elementary School Report No: Received date: Report date: Sample No: (09) 9 Rm /6/20 8//20 Sample Type: Clearance Sample Date: 8/5/20 Date Analyzed: 8//20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Sample Type: Clearance Sample Date: 8/5/20 Date Analyzed: 8//20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Sample Type: Clearance Sample Date: 8/5/20 Date Analyzed: 8//20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Asper./Pen. - like , , , 2 Alternaria spp. 3, 2, 3 Basidiospores - like 3, Particles / M 3 43 Particles / M 3 26 Particles / M Rocky Creek Rd.. Dripping Springs, Texas Tele:
32 Terracon - San Antonio 69 Blanco Rd. San Antonio, TX 826 TERRACONS69 Sample No: (0) 0 Rm. 6 Mycotech Biological, Inc. AIHA EMPAT NO: TDSHS LIC. NO: LAB063 Analysis Type : Media : Sample No: () Rm. Allergenco D Hex-Sil Project : Salinas Elementary School Report No: Received date: Report date: Sample No: (2) 2 Rm /6/20 8//20 Sample Type: Clearance Sample Date: 8/5/20 Date Analyzed: 8//20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Sample Type: Clearance Sample Date: 8/5/20 Date Analyzed: 8//20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Sample Type: Clearance Sample Date: 8/5/20 Date Analyzed: 8//20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Asper./Pen. - like , 2 3, 2 9, 2 Alternaria spp. 3, 2, 3 Basidiospores - like 3, 05 Drechslera - like 3, 2, 3, Particles / M 3 65 Particles / M 3 39 Particles / M Rocky Creek Rd.. Dripping Springs, Texas Tele:
33 Terracon - San Antonio 69 Blanco Rd. San Antonio, TX 826 TERRACONS69 Mycotech Biological, Inc. AIHA EMPAT NO: TDSHS LIC. NO: LAB063 Analysis Type : Allergenco D Media : Hex-Sil Project : Salinas Elementary School Report No: -056 Received date: 8/6/20 Report date: 8//20 Sample No: (3) 3 Outside (South) Sample Type: Clearance Sample Date: 8/5/20 Date Analyzed: 8//20 % Analyzed: 33% of Trace at 400X Magnification Reporting Limit: ,686, 2 Alternaria spp. 2 6, 2, 3 Ascospores - like 3 494, 05 Basidiospores - like 6 228, 05 Curvularia spp. 38, Nigrospora spp. 3 4 Fusarium spp. 2 6, 2, 3 4,940 Particles / M Rocky Creek Rd.. Dripping Springs, Texas Tele:
34 Project: Salinas Elementary School Report No: -056 Client: Terracon - San Antonio General Comment Reference Page ONLY COMMENT NUMBERS INDICATED ON REPORT ARE RELEVANT. Mycotech Biological is not responsible for any errors resulting from improper or incorrect sampling procedures, atmospheric conditions at the time of sampling or during shipment, or from shipping conditions or methods. relate only to samples analyzed.. This is a known and documented aeroallergen. It may cause an allergic reaction to hypersensitive individuals at normal airborne concentrations. Chronic exposure, at above normal airborne concentrations, may also result in the sensitization and development of allergic disease in previously unaffected individuals. 2. This fungus is an opportunistic pathogen. Many factors affect host contraction; however, this fungus will typically infect only those who are immuno-compromised. Immuno-compromization may be a function of age, sex, race, state of health, or nutrition. Individuals exposed to immunotherapy, chemotherapy, radiotherapy, immunosuppressant drugs, or who have contracted an immunological disorder, are at greater risk of infection. As with other diseases, opportunistic infections may be contracted by a variety of potential routes including injection, ingestion, skin contact and/or respiration. 3. Various species within this Genus/Group have been documented as producing mycotoxins. Mycotoxins represent a wide variety of secondary metabolites produced by fungi that have been documented as toxic to humans. borne mycotoxin concentrations and their specific relationship to human health has not been established to date.. The observed represented desiccated/unorganized hyphal fragments that are not representative of established fungal growth. The presence of this is commonly identified in typical dust and debris collections. Organized are the tubular filamentous parts of a fungus that represents the structural entity of the majority of the fungi. 05. Due to the absence of supporting data, a definitive Genus could not be assigned. Chris Wardlaw, B.S. PAACB Certified Spore Analyst Laboratory Manager Mycotech Biological, Inc. 6 End of Report
35
36
37 August 24, 20 Sampling Event
38 Terracon - San Antonio 69 Blanco Rd. San Antonio, TX 826 TERRACONS69 Sample No: (0) Outside (W/NW Side) Mycotech Biological, Inc. AIHA EMPAT NO: TDSHS LIC. NO: LAB063 Analysis Type : Media : Sample No: (02) 2 Rm. 9 Allergenco D Hex-Sil Project : Salinas Elementary School Report No: Received date: Report date: Sample No: (03) 3 Rm /25/20 8/25/20 Sample Type: Retest/Clearance Sample Date: 8/24/20 Date Analyzed: 8/25/20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Sample Type: Retest/Clearance Sample Date: 8/24/20 Date Analyzed: 8/25/20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Sample Type: Retest/Clearance Sample Date: 8/24/20 Date Analyzed: 8/25/20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: , 2 Asper./Pen. - like , 2 Ascospores - like 3 39, 05 3 Basidiospores - like 3, 05 Drechslera - like 2 26, 2, 3, Nigrospora spp Fusarium spp. 3, 2, 3 Torula spp Particles / M 3 9 Particles / M 3 26 Particles / M Rocky Creek Rd.. Dripping Springs, Texas Tele:
39 Terracon - San Antonio 69 Blanco Rd. San Antonio, TX 826 TERRACONS69 Sample No: (04) 4 Rm. 2 Mycotech Biological, Inc. AIHA EMPAT NO: TDSHS LIC. NO: LAB063 Analysis Type : Media : Sample No: (05) 5 Outside (W) Allergenco D Hex-Sil Project : Salinas Elementary School Report No: Received date: Report date: Sample No: (06) 6 Outside (S) /25/20 8/25/20 Sample Type: Retest/Clearance Sample Date: 8/24/20 Date Analyzed: 8/25/20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Sample Type: Retest/Clearance Sample Date: 8/24/20 Date Analyzed: 8/25/20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: 3 Sample Type: Retest/Clearance Sample Date: 8/24/20 Date Analyzed: 8/25/20 % Analyzed: 00% of Trace at 400X Magnification Reporting Limit: , , , 2 Ascospores - like 4 52, 05 Ascospores - like 2 56, 05 Basidiospores - like 3, 05 Basidiospores - like 4 52, 05 Drechslera - like 3, 2, 3, 05 Curvularia spp. 3, Nigrospora spp Nigrospora spp Particles / M 3 32 Particles / M 3 50 Particles / M Rocky Creek Rd.. Dripping Springs, Texas Tele:
40 Project: Salinas Elementary School Report No: Client: Terracon - San Antonio General Comment Reference Page ONLY COMMENT NUMBERS INDICATED ON REPORT ARE RELEVANT. Mycotech Biological is not responsible for any errors resulting from improper or incorrect sampling procedures, atmospheric conditions at the time of sampling or during shipment, or from shipping conditions or methods. relate only to samples analyzed.. This is a known and documented aeroallergen. It may cause an allergic reaction to hypersensitive individuals at normal airborne concentrations. Chronic exposure, at above normal airborne concentrations, may also result in the sensitization and development of allergic disease in previously unaffected individuals. 2. This fungus is an opportunistic pathogen. Many factors affect host contraction; however, this fungus will typically infect only those who are immuno-compromised. Immuno-compromization may be a function of age, sex, race, state of health, or nutrition. Individuals exposed to immunotherapy, chemotherapy, radiotherapy, immunosuppressant drugs, or who have contracted an immunological disorder, are at greater risk of infection. As with other diseases, opportunistic infections may be contracted by a variety of potential routes including injection, ingestion, skin contact and/or respiration. 3. Various species within this Genus/Group have been documented as producing mycotoxins. Mycotoxins represent a wide variety of secondary metabolites produced by fungi that have been documented as toxic to humans. borne mycotoxin concentrations and their specific relationship to human health has not been established to date.. The observed represented desiccated/unorganized hyphal fragments that are not representative of established fungal growth. The presence of this is commonly identified in typical dust and debris collections. Organized are the tubular filamentous parts of a fungus that represents the structural entity of the majority of the fungi. 05. Due to the absence of supporting data, a definitive Genus could not be assigned. Chris Wardlaw, B.S. PAACB Certified Spore Analyst Laboratory Manager Mycotech Biological, Inc. 3 End of Report
41
42 APPENDIX B: PHOTOGRAPHS
43 Project Name: Limited IAQ Assessment for Mold Salinas Elementary School Project No Photos Taken: August 8, 5 and 24, 20 Photo # Photo shows the Calibration Check of the flow rate (5 L/min) for the Buck Bioe Sampling Pump prior to sample collection. Photo #2 Photo shows a sample being collected from the outdoor ambient area at Salinas Elementary School. Photo #3 Photo shows an IAQ sample being collected in one of the classrooms at Salinas Elementary School. Photo #4 Photo shows what appeared to be apparent mold growth on a drawer near the sink in Rm #9 prior to remediation activities. Photo #5 Photo shows what appeared to be apparent mold growth on the restroom door in Rm #9 prior to remediation activities. Photo #6 Photo shows an IAQ sample being collected in one of the classrooms at Salinas Elementary School.
44 Project Name: Limited IAQ Assessment for Mold Salinas Elementary School Project No Photos Taken: August 8, 5 and 24, 20 Photo # Photo shows one of the UISD HEPA filtration units being used at the site. Photo #8 Photo shows one of the SERVPRO HEPA filtration units being used at the site. Photo #9 Photo shows an IAQ sample being collected in one of the classrooms at Salinas Elementary School. Photo #0 Photo shows an IAQ sample being collected in one of the classrooms at Salinas Elementary School. Photo # Photo shows an IAQ sample being collected in one of the classrooms at Salinas Elementary School. Photo #2 Photo shows a sample being collected from the outdoor ambient area at Salinas Elementary School.
45 APPENDIX C: SAMPLE LOCATION DRAWING
46
DATE: October 31, TO: Toby Maxson, Principal. SUBJECT: Heritage ES - IAQ - Initial Contact - Room B-16
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