ALGAE-BASED GREEN INGREDIENTS

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1 ALGAE-BASED GREEN INGREDIENTS

2 FROM ALGAE BASED INGREDIENTS PROFESSIONAL DIVERS 30 DIVING PER YEAR, 3 HOURS EACH LIMITED ACCESS TO THE BIOMASS COLLECTION BY BOAT HARVEST USING A «SCOUBIDOU» GREEN BROWN RED BLUE MICRO / ALGAE

3 TO ALGAE BASED GREEN INGREDIENTS We source and implement new industrial tools to master the most unexpected species and extract their unique active ingredients. GREEN EXTRACTION BIO-TECHNOLOGY PIONNER AT HEART

4 GREEN BASICS RECYCLING OF BY-PRODUCTS Parts of the algae which are unused by CODIF are used by other local industries. Process water is filtered and cleaned using a system of FILTERING GARDENS. Implementing a system to assess ENVIRONMENTAL FACTORS: e-factor, life-cycle and energy impact. 100% OF OUR POWER CONSUMPTION COMES FROM RENEWABLE SOURCES. ASSESSING ECO-TOXICITY & BIODEGRADABILITY of all active substances obtained via chemical transformation processes. Working with local players for RECYCLING SOLID PRODUCTION WASTE (ex of ALGOPACK)

5 GREEN PLAYGROUND Over 2,700 km of coastline Exceptional biodiversity They collect, isolate and identify microorganisms that produce unknown molecules. Private and protected concession. They farm algae on submerged systems. Entirely manual harvesting Guarantees an exemplary raw material. photobioreactors, enable mastering ancestral micro-algae or of calcified algae that were unable to be farmed until now. EXPLORERS GROWERS BIOTECH ENGINEERS

6 GREEN INVESTMENTS As generators of national research projects, we are dedicated to creating unique and technologically natural & green cosmetics for tomorrow's market CODIF invests in the company Polymaris. Specialist in marine biotechnologies, we became the first brand to offer a full exopolysaccharide (EPS) line. Unique innovation program started under the name POLYMER. Private companies and public labs pool their expertise to develop glyco-cosmetics for the future. HITEX, a specialist in supercritical CO2 technologies was acquired jointly in 2015 with a company that is a market leader in scented compositions.

7 GREEN SOURCING Understanding and identifying the role of each raw material within its habitat, its environment and its ecosystem. Over 6 years R&D WILD JANIA - DIVING CALCIFIED ALGA SLOW GROWTH RATE TREE OF LIFE EPIPHYTED CULTIVATED JANIA HIGHER GROWTH RATE PURE BIOMASS CONSTANT QUALITY NO ENVIRONMENTAL IMPACT

8 GREEN PLAYERS Once we master their cultivation in photobioreactors, algae and microalgae become an unlimited playground to develop unique extracts 1ST EXAMPLE Specific culture of Jania rubens for the production of vegetal taurine 2ND EXAMPLE Enrichment of seawater through the micro-alga Phormidium persicinum

9 1 ST EXAMPLE JANIA RUBENS THE DIFFERENT SOURCES OF TAURINE Cow milk 2.4ppm Human milk 42ppm Cultivated Jania: 400ppm TAURINE IS AN AMINO ACID MAINLY SOURCED IN MAMALS The cardiac muscles are strengthened in the presence of taurine, leading to improved overall function. This effect is also seen in skeletal muscles and is believed to improve exercise capacity and physical abilities. AMONG VEGETAL KINGDOM ONLY SOME RED ALGAE SYNTHESIS TAURINE It acts as an osmo-protector and is involved in calcium transfer. Thus maybe explain its high concentration in the calcified algae Jania rubens.

10 1 ST EXAMPLE JANIA RUBENS THE TECHNOLOGICAL FEAT Fed-batch cultures in photobioreactor with daily supply in vital nutriments; and specific conditions to orientate Jania s metabolism. Production rates: 20 to 50ppm of VEGETAL TAURINE in the final extract with SPECIFIC BIOAVAILABILITY for skin cells.

11 1 ST EXAMPLE JANIA RUBENS Vegetal Taurine has higher Bioavailability than synthetic Taurine % variation of mitochatp synthesis versus untreated control Control +24% Synthetic Taurine (20ppm) +33%*** 0.1% Jania extract (0.02ppm) +50%*** 0.15% Jania extract (0.03ppm) +50%*** ATP synthesis With vegetal taurine of Jania Extract. Protocol Human dermal fibroblasts from 30-year-old donor. Measure of ATP synthesis using Mito Tox Glo kit. ***p<0.001 Student test

12 2 nd EXAMPLE PHORMIDIUM Incredible adaptability to extrem environments Blue-micro algae Specific photo-protective pigments : BIOPTERIN BIOPTERIN is also: a cofactor in mitochondrial electron transfer that leads to ATP synthesis a co-factor of a powerful and universal antioxidant system: thioredoxin.

13 2 nd EXAMPLE PHORMIDIUM Enrichment of seawater in Biopterin Phormidium is cultivated in seawater in We cultivate a microalga naturally occurring in our seawater. Photobioreactor. Before the end of the culture, seawater is transfer in artic conditions (temperature <0 C) to make the alga release secondary meta bolites. Before the end of the culture, seawater is Then the microalga is removed by filtration. transfer in stressing conditions (temperature <0 C) to make the alga release secondary metabolites; among them BIOPTERIN. Then the microalga is removed by filtration, only the enriched seawater is kept for cosmetic applications

14 2 nd EXAMPLE PHORMIDIUM Tightening action of energized seawater The resulting seawater is used in a super-complex able to stimulates ATP synthesis to promote the polymerization of actin fibres. 50 years old fibroblasts + pollution 50 years old fibroblasts + pollution + Seawater Super Complex

15 GREEN EXTRACTION Supercricital CO2: Unique & Green Technology Technology based on the modulation of solvant properties of CO2 depending on temperature and pressure it is submited to. GREEN BENEFITS: - Natural solvant, inerte, non toxic - Technology that generates 0 polluting wastes - Absence of organic solvent in the final extract - Preservation of sensitive molecules - Selective extraction technology

16 SUPERCRITICAL CO2 Innovative depolymerization method: HTAC Ex of Carrageenans from red baltic algae HYDROTHERMOLYSIS ACCELERATED BY SUPERCRITICAL CO2, Subcritical water enriched with supercritical CO2. Supercritical CO2 gives subcritical water acid characteristic. fine depolymerization very high reproductibility without wastes rejections Once acidified by supercritical CO2, subcritial water promotes the depolymerization of sodium carrageenan.

17 SUPER GREEN COMPLEX WEEDING OF GREEN TECHNOLOGICAL EXPERTISE An extract of Jania rubens, rich in Taurine to energize skin cells. MARINE EXPERTISE: Algae cultivated in photobioreactor + A carrageenan with protective and instant moisturizing properties HTAC HI-TECH EXPERTISE: Marine Carrageenan depolymerized through patented HTAC technology: HydroThermolysis Accelerated by supercritical CO2.

18 GREEN OPTIMISATION OPTIMISATION OF EXISTING PROCESS TO REDUCE ENVIRONMENTAL IMPACT EX OF DERMOSCULPT Second generation of Chlorella vulgaris concentrate. While 1st generation was obtained through a classical hydrolysis process, this one has been designed using biotech tools in order to optimize our environmental impact. This is the first ECOBiotech Active Ingredient of a range REdesigned to decrease our environmental footprint. Chlorella vulgaris

19 GREEN OPTIMISATION Environmental factors selected to optimize the production of Chlorella vulgaris concentrate: WATER FOOTPRINT: production water + equipment cleaning water ENERGY CONSUMPTION: shipping of the raw materials from the supplier to our manufacturing site + energy used through the process E-FACTOR: (organic + inorganic wastes)/finished product ECO-FACTOR: (organic + inorganic + solid wastes)/finished product CLASSICAL CONCENTRATE Basic and acid extraction Filtration Acidification Filtration DERMOSCULPT Enzymatic extraction and enzymatic inactivation Filtration (Environmental and Eco Factors: Comparison of conventional and biotechnological extraction Processes. Cosm'ing 2013, Saint Malo).

20 GREEN OPTIMISATION Optimization of Chlorella vulgaris concentrate ENERGY CONSUMPTION ECO-FACTOR WATER WATER CONSUMPTI CONSUMPTION ON Classical concentrate Biotech concentrate E-FACTOR

21 WHAT S GREEN IN CODIF? PLAYGROUND INVESTMENTS SOURCING TECHNOLOGY ENGINEERS BRAND SPIRIT

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