Cement Industries. Environmental Monitoring. Food & Beverages Forensics. Agriculture

Transcription

1 Whatman TM Catalog Cement Industries Environmental Monitoring Food & Beverages Forensics Diagnostics Pharmaceuticals Agriculture Academia Filter Paper and Membranes Filtration Devices Specimen Collection Card TM All Latin America Authorised Whatman Distributors, Recommended Consumer. Ref. AWD/April 2016 gelifesciences.com/whatman 1

2 Welcome to Whatman the world s leading developer of high-end laboratory separation products. As a part of GE Healthcare, Whatman is creating a center of excellence in separations technology for the scientific community. Whether its a simple paper filter or technologies that help us understand the role of genes and proteins in disease treatments, our solutions enable scientists, engineers and medical staff to impact human lives every day from the child who needs clean drinking water to the elderly woman who needs precisely the right amount of medication to the man undergoing cancer treatment. With this, we can promote Early Health to aid earlier diagnosis and treatment of diseases. No matter what industry you are in, or what type of research you are conducting, Whatman will help you achieve your goals and touch lives. 2

3 CONTENTS Quick Product Finder 96 Alphabetical Index 97 Filter Papers and Membranes Technical Guide 8 General properties of filter papers Qualitative filter paper quick pick reference chart Quantitative filter paper quick pick reference chart Glass Microfiber filter quick pick reference chart Membranes quick reference chart Typical particle sizes Comparison of Cellulose Grades / PM 10 / PM 2.5 Cellulose filter papers Qualitative Filter Papers 9 Wet Strengthened Grades 11 Folded Prepleated Grades 12 Quantitative Filter Papers 14 Hardened Low Ash Grades 16 Hardened Ashless Grades 17 Chromatography Papers Pure Cellulose Blotting Sheets 19 Amersham Blotting Membranes 19 Amersham Protran 20 Amersham Protran Premium 20 Amersham Protran Supported 21 Amersham Hybond P 21 Amersham Hybond LFP 21 Amersham Hybond SEQ 21 Precut Membrane sandwiches 22 Amersham Hybond 22 Ion Exchange Papers 23 Application Specific Papers 23 Papers For Technical Use 24 Glass Microfilter Papers Glass Microfiber filters 25 Multigrade GMF Multigrade GMF Circles 26 Quartz Filters 28 Glass Micro Fiber Accessories 3-Piece Filter Funnel 29 Extraction Thimbles 29 Standard Cellulose Extraction Thimbles 29 High Purity Glass or Quartz microfiber thimbles 31 Glass fiber thimbles 31 Membrane Filters Track-Etched Polycarbonate and 33 Polyester Membranes Cyclopore Polycarbonate and Polyester Membranes 33 Nuclepore Track-Etched Membranes 33 Polyester Drain Discs 34 Anopore Inorganic Membranes - 34 Anodisc Aluminum Oxide Membranes for Analytical Application 35 Nylon Membranes 35 Regenerated Cellulose Membranes 35 PTFE Membranes 35 WTP and TE Membrane Ranges 36 PM 2.5 Air Monitoring Membrane 36 Membranes for Biology and Microbiology 36 Cellulose Acetate Membranes 36 Cellulose Nitrate Membranes 36 Filter Types 37 Polyamide Membranes 38 Polyethersulfone (PES) Membranes 38 Polypropylene Membranes 38 Mixed Cellulose Ester Membranes 38 Sterile Filters 38 STL Range of Membranes 39 Membrane Filter Holders 41 Syringe Type Filter holders 42 Single place vacuum filtration apparatus 42 Multiple Vacuum Filtration Apparatus 44 Pressure Filtration Devices 44 Specimen Collection Cards 903 TM Population Screening (Guthrie cards) 52 FTA Products FTA Technology 52 FTA Kits 53 Clonesaver Card 53 Easicollect Buccal sample collection device 53 FTA Elute Cards 53 FTA Accessories 54 DMPK Cards (Drug Metabolism & Pharmaco Kinetics) DMPK Cards 55 Indicating DMPK Cards

4 CONTENTS Filteration Devices Technical Guide 58 Syringe filter selection guide Membrane selection chart How to select your sample preparation device Syringe Filters GD/X Syringe Filters 63 GD/XP Syringe Filters 65 Puradisc Syringe Filters 66 Puradisc 4mm Syringe Filters 66 Puradisc 13mm Syringe Filters 67 Puradisc 25 mm Syringe Filters 68 Puradisc Aqua Anotop Syringe Filters 70 Uniflo Syringe Filters 71 Roby Syringe filters 71 Celtron 71 ReZist Syringe Filters 72 Spartan Syringe Filters 72 Syringeless Filters UniPrep Syringeless Filters 75 Autovial Syringeless Filters 75 Polydisc Filters 76 Polycap Capsule Filters 77 Venting Filters 80 Speciality Products Seed Testing Paper 85 Speciality Products Shark Skin Paper 85 Speciality Filter Paper for Textile Industry 85 Ignition Strength Papers 85 Antibiotic Assay (AA) Discs 86 Surface Protectors 86 Lens Cleaning Tissue 86 Weighing Boats 86 Specialty Filter paper 86 1 PS Phase Separators 87 ph Indicator & Test Papers 87 Multiwell Plates 88 Quick Product Finder 96 Alphabetical Index 97 Authenticity Message 99 Terms and Conditions 100 4

5 Whatman Cellulose Filter Papers: Whatman cellulose filter papers are manufactured from high-quality cotton linters, which have been treated to achieve a minimum alpha cellulose content of 98%. These cellulose filter papers are used for general filtration and exhibit particle retention levels down to 2.5 µm. There is a wide choice of retention/flow rate combinations to match numerous laboratory applications. Chromatography Papers: Whatman chromatography papers are the most widely used papers for chromatography worldwide. This acceptance and usage reflect the purity, high quality, and consistency of Whatman papers. The chromatography paper product line includes standard cellulose and ion exchange grades. Whatman Extraction Thimbles: Whatman extraction thimbles are widely used in Soxhlet extraction units, providing a safe, convenient, and efficient method of solvent extraction of solids and semi-solids. Soxhlet extraction is a widely used technique for the analysis of fats or pesticides in foods and soil materials as well as in many other procedures that involve a solid-liquid extraction. 5 5

6 Filter Papers & Membranes Filter Papers & Membranes Whatman offers an extensive line of filter papers. The innovative features of these filters make them the perfect choice for many filtering techniques. The qualitative and quantitative filter papers are manufactured from high-quality cotton linters. There is a wide choice of retention/ flow rate combinations to match numerous laboratory applications. The different groups of filter papers offer increasing degrees of purity, hardness, and chemical resistance. Whatman quantitative filter papers have extremely high purity for analytical and gravimetric work. 6

7 Technical Guide General Properties of Whatman Filter Papers Whatman Particle Initial ASTM Ash Thickness Weight Loading Wet Burst Dry Burst Grade Retention Filtration % (mm) (g/m2) Capacity kpa psi kpa psi (Liquid) Speed (Um) (Secs/100 ml) Herzberg Qualitative N N H N N N General Purpose and Wet - Strengthened NA VH NA N Ashless Quantitative N N N N N Hardened Low Ash N N N Hardened Ashless N N N Glass Microfiber GF / A NA H GF / B NA VH GF / C NA H GF / D NA VH GF / F NA H AH NA NA H EPM 2000 NA NA NA NA NA NA NA Dry Tensile (700g/1.5cm) GMF 150 (1um) NA NA VH Wet Tensile (320g//1.5cm Dry Tensile (680g/1.5cm) GMF 150 (2 um) NA NA VH Wet Tensile (333g/1.5cm) Dry Tensile (540g/1.5cm) Quartz Microfiber QM-A NA NA NA NA NA NA NA Dry Tensile (250g/1.5cm) QM-B NA NA NA NA NA NA NA Dry Tensile (400g/1.5cm) 7 7

8 Technical Guide A GUIDE TO LABORATORY FILTRATION TM Select the optimal Whatman filter for you application. Cellulose and glass filter papers for general laboratory filtration Symbols used in the selection guide: Δ Also available in prepleated version: Recommended for filtration of hard to filter samples or to increase flow rate. Θ Creped surface: increased filtration speed compared to smooth surface. µm All values in µm refer to particle retention rating at 98% efficiency. Is the suspension strongly acid or alkaline? Binder Free Glass Microfiber Filters Δ Δ Is the filter to be ashed during gravimetric analysis and/or do you require an ashless filter? Are you determining suspended solids in water? Is high wet strength required? Are you filtering under gravity or vacuum? Are you looking for a membrane pre-filter? Θ ΘΔ Δ Is the precipitate to be recovered from the filter? Binder Free Glass Microfiber Filters Do you require high loading capacity, very fine retention (<2.7µm) or a glass micro fiber filter? Binder Free Glass Microfiber Grades Whatever you need to filter, Whatman has it covered. Visit GE, imagination at work and GE monogram are trademarks of General Electric Company. 934-AH, Anotop, Whatman GD/X, GF/C, Mini-UniPrep, ReZist, SPARTAN, Whatman are trademarks of GE Healthcare companied General Electric Company All rights reserve. First published March GE Healthcare UK Limited, Amersham AA March 2014

9 Technical Guide Comparison of Cellulose Grades / PM10 / PM2.5 Trace Elements-Typical Grade Grade Grade Aluminum < Chromium Nitrogen Antimony <0.02 <0.02 Cellulose <0.02 Copper Potassium Arsenic <0.02 <0.02 <0.02 Fluorine Silicon 20 <2 <2 Barium <1 <1 <1 Iron Sodium Bromine Lead Sulfur 15 <5 <2 Calcium Magnesium Zinc Chlorine Manganese <0.05 * Qualitative Grade 1 shown for comparison Mercury <0.005 <0.005 <0.005 purposes. All results expressed as µg/g. Whatman QMA: Filters for Use in US EPA PM Ambient Air Monitoring 10 Property Test Method Unit of Measure Value Range Filter Media n/a n/a Quartz Fiber n/a Filter Thickness ASTM D mm 0.45 ± 15 Filter Dimension micrometer PM10 inches 8 x 10 ±1/16 Particle Retention (0.3 µm) ASTM D % minimum Flow Rate EPA RFM M3/min ± Tensile Strength ASTM D g/15 mm 200 minimum Brittleness Test for Fiber Filters n/a No crack larger than 1 inch maximum Integrity Test for Fiber Filters mg 2.5 maximum Weight Loss EMSL/RTP-SOP-QAD-522 %/filter 0.75 maximum Lead Content EPA RFM (40 CRF 50) µg/filter 0.25 maximum Alkalinity EPA/600/R-94/038b, Sec µeq/g of filter 25 maximum Whatman EPM 2000: Filters for Use in US EPA TSP Ambient Air Monitoring Property Test Method Unit of Measure Value Range Filter Media n/a n/a Glass Fiber n/a Filter Thickness ASTM D EPA mm 0.45 ± 15 Filter Dimension micrometer inches 8 x 10 ±1/16 Particle Retention (0.3 µm) ASTM D % minimum Flow Rate EPA RFM M3/min 1.52 ± 0.18 Tensile Strength ASTM D g/15 mm 500 minimum Brittleness Test for Fiber Filters n/a No crack larger than 1 inch maximum Integrity Test for Fiber Filters mg 2.5 maximum Weight Loss EMSL/RTP-SOP-QAD-522 %/filter 0.75 maximum Lead Content EPA RFM (40 CRF 50) µg/filter 0.25 maximum Alkalinity EPA/600/R-94/038b, Sec µeq/g of filter 25 maximum PTFE Filters for Use in US EPA PM Ambient Air Monitoring 2.5 Property Test Method Unit of Measure Value Range Filter Media n/a n/a PTFE Teflon n/a Filter Thickness micrometer µm 40 ± 10 Filter Diameter micrometer mm 46.2 ± 0.25 Filter Pore Size ASTM F PM2.5 µm 2 maximum Support Ring Media n/a n/a Polypropylene PMP or Equivalent Total Support Ring Thickness micrometer mm ± Support Ring - Width micrometer mm , Particle Retention (0.3 µm) ASTM D a % 99.7 minimum Pressure Drop (0.3µm) ASTM D a cm H liters/min. Alkalinity EPA/600-R-94/038b, Sec µeq/g of filter 25 maximum Weight loss As Above µg <20 maximum Loose Particles As Above µg <20 maximum Moisture Pickup As Above µg <10 maximum 9

10 Filter Papers and Membranes Whatman cellulose filters are manufactured from highquality cotton linters, which have been treated to achieve a minimum alpha cellulose content of 98%. These cellulose filter papers are used for general filtration and exhibit particle retention levels down to 2.5 μm. Whatman offers a wide choice of retention/flow rate combinations to suit numerous laboratory applications. Qualitative Filter Papers Grade 1: 11 μm Grade 1 standard filter paper is the most widely used filter paper for routine applications with medium retention & flow rate. This grade covers a wide range of laboratory applications and is frequently used for clarifying liquids. Traditionally, this grade is used in qualitative analytical separations for precipitates such as lead sulfate, calcium oxalate (hot), and calcium carbonate mm 500/pk mm 400/pk mm 400/pk mm 400/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 500/pk x 31mm 1000/pk x 100mm 500/pk x 570mm 100/pk x 570mm 500/pk x 600mm 100/pk x 680mm 100/pk x 680mm 500/pk Grade 2: 8 μm Grade 2 filter paper is slightly more retentive and absorbent than Grade 1 with a corresponding increase in filtration Time mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk x 570mm 100/pk x 600mm 100/pk x 680mm 100/pk 10

11 Filter Paper & Membranes Cellulose filter papers Grade 5: 2.5 μm Grade 5 filter paper is an excellent clarifying filter for cloudy suspensions and used for water and soil analysis. The maximum degree of fine particle filtration in the qualitative range. Capable of retaining the fine precipitates encountered in chemical analysis. Grade 3: 6 μm Grade 3 filter paper is double the thickness of Grade 1 with a still finer particle retention and excellent loading capacity. The extra thickness gives increased wet strength mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk x 570mm 100/pk Grade 4: μm Grade 4 filter paper is used when high flow rates in air pollution monitoring are required and the collection of fine particles is not critical mm 400/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk x 570mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk Grade 6: 3 μm Grade 6 filter paper is often specified for boiler water analysis applications. Twice as fast as Grade 5 with similar fine particle retention mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk Grade 591: 7-12 μm A thick filter paper with very high loading capacity for fast filtration of medium to coarse precipitates. This offers high absorbency and increased wet strength x580mm 250/pk 11

12 Filter Paper & Membranes Cellulose filter papers Grade 595: 4-7 μm Thin filter paper, medium-fast with medium to fine particle retention. Used for many routine analytical applications in different industries. For e.g. particle separation from food extracts or filtration of solids from digested environmental samples for ICP/AAS analysis mm 100/pk mm 100/pk mm 100/pk x580mm 500/pk Grade 597: 4-7 μm mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 1000/pk x580mm 500/pk x580mm 100/pk Grade 598: 8-10 μm A thick filter paper with high loading capacity. Combines medium retention with medium-fast to fast filtration speed mm 100/pk x580mm 250/pk Grade 602 h: < 2 μm A dense filter paper for collecting very small particles and removing fine precipitates. Used in sample preparation. For e.g., in the beverage industry for residual sugar determination, acidic spectra and HPLC mm 100/pk mm 100/pk mm 100/pk mm 100/pk Wet Strengthened Grades These extremely strong filter papers have a high wet strength due to the addition of a small quantity of chemically stable resin. Some wet strengthened grades are available in folded (prepleated) forms. Grade 91: 10 μm A general purpose creped filter for less critical routine analysis mm 4000/pk mm 4000/pk mm 1000/pk mm 1000/pk mm 1000/pk mm 1000/pk x 580mm 500/pk x 610mm 500/pk Grade 93: 10 μm mm 100/pk mm 100/pk x 580mm 500/pk x 610mm 500/pk 12

13 Filter Paper & Membranes Cellulose filter papers Grade 113: 30 μm Has an ultra high loading capacity with a particle retention making it ideal for use with coarse or gelatinous precipitates. Thickest paper & fastest flow rate of the qualitative grades with a creped surface mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk x 570mm 100/pk Grade 114: 25 μm Half the thickness of Grade 113 with a smooth surface for easy recovery of precipitates mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk Grade cm 100/pk Grade 1573: μm A fast filter paper with high wet strength. It has a very smooth surface, making iteasy to scrape or wash off precipitate. Resistant against: sulfuric and nitric acid solutions (up to 40% at 50 C), hydrochloric (up to 10% at 100 C, 20% at 60 C, 25% at 20 C) and alkalis (up to 10% at 20 C). Cat. No. Diameter mm Qtz mm 100/pk mm 100/pk mm 100/pk Grade 1575: < 2 μm Slow filter paper with high wet strength. This paper has the same chemical resistance characteristics as Grade mm 100/pk Folded Prepleated Grades Time-saving Whatman qualitative grades are offered in this convenient form, which have major advantages over flat circles: Saves time required to quadrant-fold circles to fit conical filter funnels in repetitive or multiple analyses Decreased overall filtration time because of the extra surface area exposed Increased total loading capacity as more filter area is available Maintained flow rate due to the reduction in filter paper contact with funnel side and the self-supporting shape of the filter itself. Grade 2V: 8 μm mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk Grade 1V V 12.5CM 100/PK V 18.5CM 100/PK V 27CM 100/PK 13

14 Filter Paper & Membranes Cellulose filter papers Grade 4V V 12.5CM 100/PK V 18.5CM 100/PK V 27CM 100/PK Grade 113V: 30 μm mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk Grade 114V: 25 μm mm 100/pk mm 100/pk mm 100/pk mm 100/pk Grade 287 ½ mm 50/pk mm 50/pk mm 50/pk Grade 520 a ½: μm mm 100/pk mm 100/pk x 580mm 250/pk Grade 520 b ½: μm mm 50/pk mm 50/pk mm 50/pk mm 50/pk mm 20/pk x 580mm 250/pk 14 Grade mm 100/pk mm 100/pk Grade mm 100/pk Grade 595 ½: 4-7 μm mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk Grade 597 ½: 4-7 μm mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk Grade 598 ½: 8-10 μm mm 50/pk mm 50/pk mm 50/pk mm 50/pk

15 Filter Paper & Membranes Cellulose filter papers Grade 602 h ½: < 2 μm mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk Grade 604 ½ mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk Grade 1573 ½: μm mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk Grade 1574 ½: 7-12 μm mm 100/pk Grade 1406 ½ mm 100/pk Quantitative Filter Papers Ashless Grades 0.007% ash maximum for Grades 40 to 44 and a maximum of 0.01% for the 589 Grades - Very pure filters suitable for a wide range of critical analytical filtration procedures. Grade 40: 8 μm The classic general purpose ashless filter paper with medium speed and retention. Typical applications include gravimetric analysis for numerous components in cements, clays, iron, and steel products mm 400/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk x 570mm 100/pk Grade 41: 20 μm The fastest ashless filter paper, recommended for analytical procedures involving coarse particles or gelatinous precipitates. Gravimetric analysis, general soil analysis, quantitative determination of sediments in milk, analytical clean-up filter mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk x 254mm 100/pk x 570mm 100/pk 15

16 Filter Paper & Membranes Cellulose filter papers Grade 42: 2.5 μm This paper contains the finest particle retention of all Whatman cellulose filter papers. Typical analytical precipitates include barium sulfate and finely precipitated calcium carbonate mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk x 570mm 100/pk Grade 43: 16 μm Food and soil analysis, air pollution monitoring and inorganic analysis in the construction, mining and steel industries mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk Grade 44: 3 μm mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk Black Ribbon Filter the established standard in quantitative analysis for the filtration of coarse precipitates (class 2a acc. to DIN ) mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk Grade 589/2: 4-12 μm White Ribbon Filter ashless standard filter paper for medium fine precipitates (class 2b acc. to DIN ) offering medium filtration speed mm 1000/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk Grade 589/2 ½ mm 100/pk mm 100/pk Grade 589/1: μm 16

17 Filter Paper & Membranes Cellulose filter papers Grade 589/3: 2 μm Blue Ribbon Filter ashless standard filter paper for very fine precipitates (class 2d acc. to DIN ). Slow filter paper with highest efficiency for collecting very small particles mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 1000/pk Hardened Low Ash Grades The maximum ash content of these grades is intermediate between ashless and qualitative grades. They are particularly suitable for Buchner filtrations where it is desirable to recover the precipitate from the filter surface after filtration. Other characteristics include high wet strength and chemical resistance, which are similar to the acid hardened ashless filter papers. Grade 52: 7 μm mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk Grade 50: 2.7 μm Retention of very fine crystalline precipitates. The thinnest of all Whatman filter papers with a slow flow rate. Hardened and highly glazed surface. This finish also keeps the paper free from loose surface fibers. Highly suitable for qualitative or quantitative filtrations requiring vacuum assistance on Büchner or 3-piece filter funnels mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk x 570mm 100/pk Grade 54: 22 μm mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk x 570mm 100/pk 17

18 Filter Paper & Membranes Cellulose filter papers Hardened Ashless Grades These are the supreme quantitative filter papers featuring high wet strength and chemical resistance. These papers are acid hardened, which reduces ash to an extremely low level. Their tough surfaces make them suitable for a wide range of critical analytical filtration operations. Each grade offers a convenient combination of filtration speed and particle retention. Grade 540: 8 μm This is a general purpose hardened ashless filter paper with medium retention and flow rate. Extremely pure and strong with a hard surface. High chemical resistance to strong acid and alkali. Frequently used in the gravimetric analysis of metals in acid/alkali solutions and in collecting hydroxides after precipitation by strong alkalis mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk x 570mm 100/pk Grade 542: 2.7 μm High retention of fine particles under demanding conditions. Slow flow rate with excellent chemical resistance mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk Ashless Clippings g 1/pk Grade 541: 22 μm Fast filtration of coarse particles and gelatinous precipitates in acid/alkali solutions during gravimetric analysis mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk 18

19 Filter Paper & Membranes Chromatography Papers Chromatography Papers Whatman chromatography papers are the most widely used papers for chromatography worldwide. Features & benefits Pure cellulose produced entirely from the highest quality cotton linters with no additives of any kind Manufactured and tested specifically for chromatographic techniques this ensures the wicking capability and uniformity of capillary action that are important in chemical separations Also widely used in protein and nucleic acid blotting. Grade 1 CHR The world standard chromatography paper. Good resolution for general analytical separations mm x 100mm 1/pk mm x 100mm 1/pk mm x 100mm 1/pk mm x 100m 1/pk mm x 100mm 1/pk mm x 100mm 1/pk mm x 100mm 1/pk x 300mm 100/pk x 200mm 100/pk x 250mm 100/pk x 570mm 100/pk x 680mm 100/pk Grade 2 CHR Recommended for optical or radiometric scanning x 570mm 100/pk Grade 3 CHR A medium thickness paper generally used for general applications with medium/heavy solute loadings. Frequently used for separation of inorganic compounds and for electrophoresis x 570mm 100/pk Recommended for routine chromatography when loadings are relatively low. This is suitable where speed is important and very high resolution is not required mm x 100mm 1/pk x 570mm 100/pk Grade 17 CHR Suitable for the heaviest loadings and for preparative paper chromatography and electrophoresis x 90mm 100/pk x 570mm 25/pk x 570mm 100/pk Grade 20 CHR Recommended for separation of samples of unknown composition, with outstanding resolution at low loadings x 570mm 100/pk Grade 31ET CHR Thick paper with highest flow rate. Principal application is in electrophoresis of large molecules cm x 100cm 1/pk x 50mm 1000/pk x 570mm 25/pk Grade 2668 Grade 4 CHR 19

20 Filter Paper & Membranes Chromatography Papers x 600mm 100/pk Grade mm 100/pk x 600mm 50/pk x 190mm 100/pk Grade 3MM CHR Though widely used as a blotting paper, 3MM Chr is also used both in electrophoresis and for general chemistry x175 pk/ x340 pk/ x355 pk/ x430 pk/ x450 pk/ x140 pk/ x100M pk/ x140 pk/ "x 8" pk/ x200 pk/ "x 5.25" pk/ x410 pk/ x100M pk/ x100M pk/ x100M pk/ x100M pk/ x100M pk/ x100M pk/ x100m pk/ x200 pk/ x254 pk/ x57CM pk/ x68CM pk/100 Pure Cellulose Blotting Sheets GB 003 Blotting paper with a high absorbency used as a membrane gel support recommended for the lysis/ denaturation of colony or plaque lifts and Western blots x x x x x x x x GB 005 A thick (1.5 mm) highly absorbent paper recommended for applications where fewer layers of blotting paper must still ensure a high capacity. Recommended for semi-dry blotting of proteins x x x Amersham Blotting Membranes Whatman offers a broad selection of nitrocellulose (NC) and poly-vinylidinedifluoride (PVDF) Western blotting membranes, with pore sizes ranging from 0.1 to 0.45 μm to suit your application requirements. The main advantage of NC membranes is the low background, regardless of detection method used. The low background makes NC membranes well-suited for applications where X-ray film is used for detection. In general, NC membranes are not recommended for stripping and reprobing, as they become brittle and difficult to handle when dry. However, AmershamProtran Supported NC membranes have an incorporated polyester support web for improved mechanical strength, allowing for stripping and re-probing of the membrane. PVDF membranes have higher mechanical strength than NC membranes and, thus, are more suitable for Western blotting applications where stripping and reprobing is needed. In addition, PVDF membranes exhibit higher protein binding 20

21 Filter Paper & Membranes Chromatography Papers capacity, however, with a tendency for a higher background, compared with NC membranes. For good results, a CCD imager is recommended for signal detection. Amersham Protran NC 300mm*4m 1 roll/pk NC 300mm*4m 1 roll/pk NC 300mm*4m 1 roll/pk NC 200mm*4m 1 roll/pk NC 200mm*4m 1 roll/pk NC 200mm*4m 1 roll/pk NC 150mm*4m 1 roll/pk NC 150mm*4m 1 roll/pk NC 150mm*4m 1 roll/pk NC 300mm*600mm 5/PK NC 300mm*600mm 5/PK NC 300mm*600mm 5/PK NC 200mm*200mm 25/PK NC 200mm*200mm 10/PK NC 200mm*200mm 25 sheets/pk NC 200mm*200mm 10 sheets/pk NC 200mm*200mm 25 sheets/pk NC 200mm*200mm 10 sheets/pk NC 140mm*160mm 25/PK NC 140mm*160mm 25 sheets/pk NC 140mm*160mm 25 sheets/pk NC 100mm*100mm 25 sheets/pk NC 80mm*90mm 25/PK NC 100mm*100mm 10 sheets/pk NC 100mm*100mm 25 sheets/pk NC 100mm*100mm 10 sheets/pk NC 100mm*100mm 25 sheets/pk NC 100mm*100mm 10 sheets/pk NC 80mm*90mm 25 sheets/pk NC 80mm*90mm 25 sheets/pk NC membranes with low background and high sensitivity and resolution, recommended for fluorescent detection systems mm x 4m 1 Roll mm x 4m 1 Roll mm x 4m 1 Roll/PK mm x 4m 1 Roll/PK mm x 4m 1 Roll/PK mm x 4m 1 Roll/PK mm x 600mm 5/PK mm x 600mm 5/PK mm x 200mm 25/PK mm x 200mm 10 sheets mm x 200mm 25/PK mm x 200mm 10/PK mm x 160mm 25/PK mm x 160mm 25/PK mm x 100mm 25/PK mm x 100mm 10/PK mm x 100mm 25/PK mm x 100mm 10/PK mm x 90mm 25 sheets mm x 90mm 25 sheets Amersham Protran Supported NC membranes supported by an inert polyester non-woven material, giving the membranes exceptional handling characteristics that allows for stripping and reprobing of the membrane mm x 4m 1 Roll/PK mm x 4m 1 Roll/PK mm x 4m 1 Roll/PK mm x 4m 1 Roll/PK Amersham Protran Premium 21

22 Filter Paper & Membranes Chromatography Papers mm x 4m 1 Roll/PK mm x 4m 1 Roll/PK mm x 600mm 5/PK mm x 600mm 5/PK mm x 200mm 25/PK mm x 200mm 25/PK mm x 200mm 10/PK mm x 200mm 10/PK mm x 160mm 25/PK mm x 160mm 25/PK mm x 100mm 25/PK mm x 100mm 10/PK mm x 100mm 25/PK mm x 100mm 10/PK mm x 90mm 25/PK mm x 90mm 25 sheets Amersham Hybond P PVDF membranes with low background and high sensitivity and resolution, for all labeling and detection systems, but particularly recommended for chemiluminescence detection µm PVDF 1 roll/pk 300mmx4m µm PVDF 1 roll/pk 150mmx4m µm PVDF 5/PK 300mmx600mm µm PVDF 25/PK 140mmx160mm µm PVDF 25/PK 100mmx100mm µm PVDF 10/PK 100mmx100mm µm PVDF 80mmx90mm 25 Sheets Amersham Hybond LFP PVDF membranes specifically optimized for fluorescence detection methods PVDF 254 mm*4m 1 roll/pk PVDF 5/PK 254mm*600mm PVDF 10 sheets 200mm*200mm PVDF 25 sheets 100mm*100mm PVDF 10 sheets 100mm*100mm PVDF 80mm*90mm 25 sheets/pk Amersham Hybond SEQ PVDF membranes specifically designed for protein sequencing applications and solid-phase amino acid analysis PVDF 260mm*4m 1 roll/pk PVDF 10 sheets 100mm*100mm PVDF 200mm*200mm 10 sheets Precut Membrane sandwiches µm NC + 3MM Chr 10+20/PK Paper 140mmx160mm µm NC + 3MM Chr Paper 140mmx160mm 10+20/PK 22

23 Filter Paper & Membranes Chromatography Papers µm NC + 3MM Chr Paper 140mmx160mm µm NC + 3MM Chr Paper 140mmx160mm µm NC + 3MM Chr Paper 140mmx160mm µm NC + 3MM Chr Paper 140mmx160mm µm NC + 3MM Chr Paper 140mmx160mm µm NC + 3MM Chr Paper 80mmx90mm µm NC + 3MM Chr Paper 140mmx160mm µm NC + 3MM Chr Paper 140mmx160mm µm NC + 3MM Chr Paper 80mmx90mm µm NC + 3MM Chr Paper 80mmx90mm µm NC + 3MM Chr Paper 80mmx90mm µm NC + 3MM Chr Paper 80mmx90mm µm NC + 3MM Chr Paper 80mmx90mm µm PVDF + 3MM Chr Paper 80mmx90mm µm PVDF + 3MM Chr Paper 80mmx90mm µm NC + 3MM Chr Paper 80mmx90mm µm PVDF + 3MM Chr Paper 140mmx160mm µm PVDF + 3MM Chr Paper 80mmx90mm 10+20/PK 10+20/PK 10+20/PK 10+20/PK 10+20/PK 10+20/PK 10+20/PK 10+20/PK 10+20/PK 10+20/PK 10+20/PK 10+20/PK 10+20/PK 10+20/PK 10+20/PK 10+20/PK 10+20/PK 10+20/PK Our Western blotting transfer membrane family is composed of high-quality polyvinylidene fluoride (PVDF) microporous membranes available as rolls, sheets, or as readymade sandwiches together with our high-grade filter papers. PVDF membranes retain proteins very strongly during transfer but also reduce nonspecific protein binding that can obscure highsensitivity detection. PVDF membranes are also an excellent choice for Northern and Southern blotting of nucleic acids PVDF 260mm*4m 1 roll/pk PVDF 10 sheets/pk 200mm*200mm PVDF 10 sheets/pk 100mm*100mm PVDF 5/PK 260mm*600mm PVDF 25 sheets/pk 100mm*100mm PVDF 80mm*90mm 25 sheets/pk PVDF 200mm*200mm 25 sheets/pk Ion Exchange Papers Simultaneous development of multiple samples on the same sheet under identical conditions and development of samples with different solvents and/or different concentrations of the same solvent Suitable for two-dimensional chromatography (change in direction of the solvent front) with possible improved resolution Used in enzyme assays to separate product from reactant by charge or polarity SG 81 A novel paper combining cellulose and large pore silica gel. This paper is not charged, but binds polar molecules from lesspolar solvent. Suitable for separations in which both partition and adsorption are important, including the separation of phospholipids, steroids, phenols and dyes x 57CM 25/pk Amersham Hybond Application Specific Papers Grade 2 (IS Certified) Certified for use in measuring the ignition strength of cigarettes according to ASTM standard E

24 Filter Paper & Membranes Chromatography Papers mm 100/pk Grade 0048 Filter mat made from a mixture of cellulose and polyester. This mat is used for optically testing baby food (artificial milk) for textile fibers mm 1000/pk Grade 72 Composite cellulose/glass filter loaded with activated carbon. Used to absorb radioactive iodine in air pollution monitoring and in nuclear installations mm 100/pk Grade 2555 ½ A medium fast filter paper. Used for the filtration of the mash for the determination of the extract in malt and wort and for removing carbon dioxide from beer. Prepleated mm 100/pk mm 100/pk Grade 0790 ½ Acid-washed paper with ash content of approximately 0.01%, low magnesium, and phosphorus for the determination of trace elements (Mg,Mn, Co, Cu, Mo, B) mm 100/pk mm 100/pk Grade 512 1/2 Low phosphate papers approximately 1.5 ppm phosphate, for the filtration of calcium lactate extracts from soil samples for the determination of K and P mm 100/pk Grade 3000 For sugar/food industry mm 1000/pk Grade 3459 For sugar/ food industry mm 1000/pk Papers For Technical Use Grade 0858: 7-12 µm A universal filter paper used for the filtration of extracts, oils, beer, syrups, etc., also applied in filter presses or for the aspiration of liquids mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk x 580mm 500/pk x390mm 500/pk x450mm 500/pk Grade 860: 12 µm Comparable to 0858 but with a smooth surface, slightly thinner and faster mm 100/pk mm 100/pk mm 100/pk mm 100/pk 24

25 Filter Paper & Membranes Cellulose filter papers mm 100/pk Grade 903: 4-7 µm Thin filter paper with smooth surface. Offers medium to slow flow rate and good retention for small particles x450mm 500/pk Grade 905: µm A creped paper for coarse particles, offers a very high filtration speed x 450mm 500/pk x 580mm 500/pk Grade 2294: 8-15 µm A very thick filter card with high wet strength. Offers very high flow rate and retains medium to coarse particles mm 100/pk mm 100/pk mm 100/pk Grade 2589 A fast to medium fast filter with high wet strength offering medium retention mm 500/pk x 580mm 100/pk x 75mm 100/pk x 75mm 100/pk 25

26 Filter Paper & Membranes Glass Microfiber filters Glass Microfiber filters These depth filters combine fast flow rates with high loading capacity and the retention of very fine particles, Stable upto 500 o C. Binder Free glass microfiber filters Whatman glass microfiber filters are manufactured from 100% borosilicate glass. Binder free grades can withstand temperatures up to 550 o C and can therefore be used in gravimetric analysis. Grades such as GF/B or GF/D are recommended because of low resistance to fluid flow and high particle loading capacity. Grade GF/A (1.6 µm) Used for high-efficiency general purpose laboratory filtration, monitoring water pollution, filtration of water, algae and bacteria cultures, foodstuff analyses, protein filtration, and radio immunoassay of weak ß emitters. Recommended for gravimetric determination of airborne particulates, stack sampling, and absorption methods of air pollution monitoring mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk x 254 mm 100/pk x 570mm 25/pk Grade GF/B (1.0 µm) Can be used as a finely retentive membrane prefilter. Used in LSC techniques where high loading capacity is required mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 25/pk mm 25/pk mm 25/pk mm 25/pk mm 25/pk x 570mm 5/pk x 570mm 25/pk Grade GF/C (1.2µm) Collection of suspended solids in potable water and natural and industrial wastes. Widely used for cell harvesting, liquid scintillation counting, and binding assays mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk x 254mm 50/pk x 10 inch 100/pk x 570mm 25/pk 26

27 Filter Paper & Membranes Glass Microfiber filters Grade GF/F (0.7 µm) Recommended for DNA binding and purification. Very effective in filtering finely precipitated proteins, GF/F can be used in conjunction with GF/D as a prefilter for the successful clarification of extremely difficult biochemical solutions and fluids, and nucleic acids. Grade GF/D (2.7 µm) GF/D will provide good protection for finely retentive membranes. Can be used in combination with GF/B to provide very efficient graded prefilter protection for membranes mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 25/pk mm 25/pk mm 25/pk mm 25/pk mm 25/pk mm 25/pk x 570mm 25/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 25/pk mm 25/pk mm 25/pk mm 25/pk mm 25/pk mm 25/pk x 570mm 25/pk Multigrade GMF 150 Multiple Porosities, Greater Filtration Efficiency Whatman GMF 150 is a binder free glass microfiber filter with a coarse top layer (10 µm) meshed with a finer layer of 1 µm or 2 µm. The GMF 150 allows for: Higher particulate loading capacity Faster flow rate Extended life of filter Multigrade GMF Circles mm 40/pk mm 20/pk mm (2µm) 40/pk mm (2µm) 20/pk 27

28 Filter Paper & Membranes Glass Microfiber filters Grade 934-AH A high retention borosilicate glass microfiber filter, which withstands temperatures over 500 C. Grade 934-AH is used for a wide range of laboratory applications. It is recommended for water pollution monitoring, cell harvesting, liquid scintillation counting, and air pollution monitoring. Acid treated low metal TCLP filters Used for EPA Method 1311, Toxicity Characteristic Leaching Procedure (TCLP) is an analytical test designed to determine the leaching potential in a landfill for hazardous organic and inorganic contaminants that could potentially migrate into groundwater, threatening drinking water sources. The Whatman TCLP Filter is manufactured using a binder free borosilicate glass microfiber with a particle retention rating of 0.6 to 0.8μm mm 100/pk mm 50/pk mm 50/pk mm 50/pk mm 50/pk mm 50/pk Grade EPM 2000: 2.0 µm EPM 2000 has been developed especially for use in high volume air sampling equipment that collects atmospheric particulates and aerosols. It is manufactured from 100% pure borosilicate glass of special purity, enabling detailed chemical analysis of trace pollutants to take place with the minimum of interference or background mm 100/pk x 10 inches 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk x 10 inches 100/pk x 28 inches 100/pk Grade 934 RTU mm 100/PK mm 100/PK mm 100/PK 28

29 Filter Paper & Membranes Glass Microfiber filters Quartz Filters High-purity quartz (SiO 2 ) microfiber filters are used for air sampling in acidic gases, stacks, flues, and aerosols, particularly at high temperatures up to 500 C and in PM-10 testing. QM-A, sequentially numbered according to EPA standards, is suitable for most applications. Grade QM-A: 2.2 µm mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk x 10 inches 25/pk x 10 inches 100/pk Grade QM-H Pure quartz fiber filter with low heavy metal content, can be used at temperatures up to 900 o C mm 25/pk QM-H 50MM 50/PK QM-H 90MM 50/PK QM-H 47MM 50/PK QM-H 150MM 50/PK Glass Microfiber filters with binder Grade GF 6 - Inorganic Binder This filter is used in water pollution applications, for removing protein from difficult-to-filter beers, for determination of chlorophyll and phytoplankton residues, for the determination of filterable substances and the residue on ignition (dry weight), for the analysis of aggressive media (e.g., acidic gases), for scintillation measurements, and for determination of the elemental iron content in the presence of iron oxides mm 200/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 200/pk mm 200/pk mm 100/pk x 620mm 100/pk Grade GF 8 - Inorganic Binder This glass fiber filter is used in the filtration of coarse particles. Frequently used in environmental analysis, in the determination of PCB, DDE, DD T, furans and dioxins in the air; pollution measurements in industrial, urban and populated areas, cement factories, iron and steel industry, dust measurements at the workplace, and testing the effectiveness of dust collecting mm 100/pk mm 200/pk x 90mm 100/pk Glass fibre filter with binder ~ 3mm Retention capability < 1mm GF8 GF9 GF10 GF3362 GF6 Fast Medium Slow Filtration Speed Grade GF 9 - Inorganic Binder mm 200/pk mm 100/pk mm 100/pk 29

30 Filter Paper & Membranes Glass Micro Fiber Accessories / Extraction Thimbles Grade GF 10 - Organic Binder This filter with extreme mechanical stability and temperature resistant up to 180 C is used as a weighing aid for infrared weighing mm 200/pk mm 100/pk mm 100/pk mm 100/pk mm x 100m 1/pk mm x 30m 1/PK mm 1/pk mm 1/pk mm 1/pk mm 1/pk mm 1/pk mm 1/pk mm 1/pk mm 1/pk mm 1/pk mm 1/pk mm 1/pk mm 1/pk mm 1/pk Extraction Thimbles GF 92 - Inorganic Binder mm 200/pk mm 200/pk mm 200/pk mm 100/pk mm 100/pk mm 100/pk Glass Micro Fiber Accessories 3-Piece Filter Funnel Whatman cellulose and glass microfiber extraction thimbles are known for their purity and consistent high quality. The thimbles are widely used in Soxhlet extraction units, providing a safe, convenient, and efficient method of solvent extraction of solids and semi-solids. Soxhlet extraction is a widely used technique for the analysis of fats or pesticides in foods and soil materials as well as in many other procedures that involve a solid-liquid extraction. Standard Cellulose extraction thimbles Standard single thickness thimbles have a wall thickness of approximately 1 mm (10.0 μm nominal particle retention). Double thickness thimbles have a wall thickness of approximately 2 mm (6.0 μm nominal particle retention) for applications where higher retention and increased wet or dry Whatman 3-Piece Filter Funnels have been designed to complement the range of fine particle retention, rapid flow rate glass microfiber filters. Also available with a choice of three plates and several sizes. 30

31 Filter Paper & Membranes Extraction Thimbles strength, or rigidity are required. Thimbles of type 603 are made from high quality cellulose x 80mm 25/pk x 60mm 25/pk x 80mm 25/pk x 100mm 25/pk x 60mm 25/pk x 80mm 25/pk x 60mm 25/pk x 80mm 25/pk x 100mm 25/pk x 80mm 25/pk x 100mm 25/pk x 60mm 25/pk x 80mm 25/pk x 90mm 25/pk x 94mm 25/pk x 100mm 25/pk x 118mm 25/pk x 130mm 25/pk x 205mm 25/pk x 130mm 25/pk x 150mm 25/pk x 85mm 25/pk x 123mm 25/pk x 145mm 25/pk x 200mm 25/pk x 230mm 25/pk x 250mm 25/pk x 60mm 25/pk x 80 x 250mm 25/pk HP Cellulose extraction Thimbles a x 50mm 25/pk x 55mm 25/pk x 90mm 25/pk x 65mm 25/pk x 80mm 25/pk x100mm 25/pk x 80mm 25/pk x 90mm 25/pk x 100mm 25/pk x 60mm 25/pk x 100mm 25/pk x 120mm 25/pk x 80mm 25/pk x100mm 25/pk x 80mm 25/pk x 100mm 25/pk x118mm 25/pk x 80mm 25/pk x 94mm 25/pk x 130mm 25/pk x 123mm 25/pk x 123mm 25/pk x 180mm 25/pk , x 60mm 25/pk x 80mm 25/pk x 80mm 25/pk x 60mm 25/pk x 80mm 25/pk x 123mm 25/pk High Purity Glass or Quartz microfiber 31

32 Filter Paper & Membranes Extraction Thimbles thimbles High-purity glass microfiber thimbles are manufactured from 100% pure borosilicate glass are available for specializedapplications. The thimbles are completely free of binders or additives and can be used at temperatures up to 500 C or when using solvents that are incompatible with cellulose thimbles. These thimbles are also used in pollution monitoring techniques. Features and Benefits Available in a range of sizes and wall thicknesses to suit your application Designed to fit most commercially available Soxhlet extractors No binders are added Applications Smoke stack gas monitoring. Soxhlet extraction. Analyzing pesticide residues. Determining oil/fat content of foods (e.g., French fries). Analysis of oil and grease in solid wastes. Grade HP-GF x 90mm 25/pk x 90mm 25/pk x 100mm 25/pk x 123mm 25/pk x 80mm 25/pk Glass fiber thimbles Grade 603g Thimbles of type 603 g are made from borosilicate glass fibers with inorganic binder x 50mm 25/pk x 90mm 25/pk x 80mm 25/pk x 100mm 25/pk x 100mm 25/pk x 60mm 25/pk x 100mm 25/pk x 94mm 25/pk x 118mm 25/pk x 150mm 25/pk x 38mm 25/pk x 68mm 25/pk Dionex ASE Extraction thimbles 25/pk for DIONEX ASE 200 extraction cell 11ml Extraction thimbles for DIONEX ASE 200 extraction cell 22ml 25/pk Quartz & Silca Thimbles (Tappered) x 90mm 10/pk 32

33 Filter Paper & Membranes Membranes Membrane Filters Whatman brings to the laboratory user a range of membrane filters whose advanced technical specifications make them today s preferred choice for a wide range of applications. The membrane filters offer accurately controlled pore size distribution and higher strength and flexibility, which ensure reproducibility and consistency. The Whatman membrane filter range includes pore sizes from to 12 μm with a wide selection of membrane filters. Sterile and autoclave packs are available for specialized applications. Colored and gridded types are also available. Quick Pick Reference Chart Sample Membrane Liquids Air Aqueous Solvents Cell Culture Media PES Non-polar (Hydrophobic) PTFE General Purpose Microbiology Nitrocellulose Particle Analysis Microbiology Mixed Cellulose Ester Particle Analysis SEM Epifluorescence Track-Etched General Purpose Filtration Low Protein Binding Applications Cellulose Acetate/Regenarated Cellulose Polar (Hydrophilic) Nylon PTFE Mixed Cellulose Ester Track-Etched / Polycarbonate RC Solvent Mixtures Nylon PTFE RC 33

34 Filter Paper & Membranes Membranes Track-Etched Polycarbonate and Polyester Membranes Whatman offers a complete range of track-etched membranes manufactured using proprietary Whatman technology to produce a precision membrane filter with a closely controlled pore size distribution. These membranes include Cyclopore polycarbonate and polyester, Nuclepore polycarbonate, chemotaxis membranes, black polycarbonate, and polycarbonate membranes for cell culture. Cyclopore Polycarbonate and Polyester Membranes Whatman Cyclopore membranes are true pore size microporous membranes featuring sharp cut-off and reproducible microfiltration performance characteristics of track-etched membranes. The smooth flat membrane ensures particles are retained on the surface so that they are easily visible under a microscope. Cyclopore membranes are manufactured using proprietary Whatman technology to produce a precision membrane filter with a closely controlled pore size distribution. Features and Benefits Low affinity for stains providing higher optical contrast and making visibility under a microscope easy True surface capture provides easy examination of samples and short analysis times Totally transparent membranes available Negligible absorption and adsorption of filtrate; nonhygroscopic Low tare weights No particle shedding provides ultra clean filtrate Biologically inert Cyclopore PC Membrane µm 13mm 100/pk µm 25mm 100/pk µm 25mm 100/pk µm 25mm 100/pk µm 47mm 100/pk µm 47mm 100/pk µm 47mm 100/pk µm 47mm 100/pk µm 25mm 100/pk Nuclepore 34 Track-Etched Membranes Nuclepore track-etched polycarbonate membranes are manufactured from high-quality polycarbonate film and have sharply defined pore sizes, high flow rates, and excellent chemical and thermal resistance. The membranes have a smooth flat surface and exhibit very low levels of extractables. Features and Benefits Low protein binding and low extractables, ensuring no sample contamination High chemical resistance and good thermal stability for a wide range of samples Low, consistent ash and tare weights Smooth flat surface for good visibility of particles Cat. No. Diameter mm, Qty Pore Size mm 0.1µm 100/pk mm 0.8µm 100/pk mm 0.05µm 100/pk mm 0.08µm 100/pk mm 0.1µm 100/pk mm 0.2µm 100/pk mm 0.4µm 100/pk mm 0.8µm 100/pk mm 1.0µm 100/pk mm 2.0µm 100/pk mm 3.0µm 100/pk mm 5.0µm 100/pk mm 8.0µm 100/pk mm 10.0µm 100/pk AOX 25mm 0.4µm 100/pk Black 25mm 0.2µm 100/pk Black 25mm 0.8µm 100/pk mm 0.8µm 100/pk mm 0.015µm 100/pk mm 0.05µm 100/pk mm 0.08µm 100/pk mm 0.1µm 100/pk mm 0.2µm 100/pk mm 0.4µm 100/pk mm 0.6µm 100/pk mm 0.8µm 100/pk mm 1.0µm 100/pk mm 2.0µm 100/pk

35 Filter Paper & Membranes Membranes mm 3.0µm 100/pk mm 5.0µm 100/pk mm 8.0µm 100/pk mm 10.0µm 100/pk mm 0.2µm 100/pk mm 12.0µm 100/pk mm 0.1µm 25/pk mm 0.2µm 25/pk mm 1.0µm 25/pk mm 3.0µm 25/pk mm 0.08µm 25/pk mm 0.2µm 25/pk x 42mm 5.0µm 100/pk x 10 inches 0.03µm 25/pk x 10 inches 0.2µm 25/pk NUC PC 13MM 8.0uM PVPF 100/pk Nuclepore Gold 13mm 0.8µm 10/pk Nuclepore PC 19mm 0.2µm 100/pk Nuclepore PC 19mm 0.40µm 100/pk Nuclepore PC 19mm 0.05µ 100/pk Nuclepore PC 19mm 0.1µm 100/pk Polyester Drain Discs For use with membrane hardware where extra support is needed for improved flow rate and throughput. The polyester drain disc is binder free and has a thickness of 100 μm. It provides a flat surface to eliminate filter tearing or rupturing. It is also used as a separator between membrane layers in serial stack filtration applications. This chemically inert support disc is available in a variety of diameters for use in a range of devices mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk Anopore Inorganic Membranes - Anodisc Aluminum Oxide Anopore inorganic membrane is composed of a highpurity alumina matrix that is manufactured electrochemically. The membrane also exhibits low protein binding, has minimal autofluorescence, is nontoxic, and supports cellular growth. The membrane is hydrophilic and is compatible with most solvents and aqueous material. No monomers, plasticizers, adhesives, surfactants or wetting agents are used in the manufacturing process, which eliminates sample contamination and ensures low protein binding and minimal loss of sample mm 0.02µm 100/pk Supported mm 0.1µm Supported 100/pk mm 0.2µm Supported 100/pk mm 0.02µm 50/pk Unsupported mm 0.02µm 50/pk Supported mm 0.1µm Supported 50/pk mm 0.2µm Supported 50/pk mm 0.02µm 50/pk Unsupported mm 0.1µm 50/pk Unsupported mm 0.2µm Unsupported 50/pk Membranes for Analytical Application Nylon Membranes High-quality nylon membranes are suitable for filtering aqueous solutions and most organic solvents. The membranes are suitable for use with a wide range of biological preparations and can be used where other membranes are unsuitable or difficult 35

36 Filter Paper & Membranes Membranes to use. Nylon membranes are hydrophilic, eliminating the need for wetting agents that could be extracted when filtering aqueous solutions. The membranes are flexible, durable and tear resistant, and can be autoclaved at 135ºC. Applications Filtration of aqueous and organic mobile phases Vacuum degassing Filtration of tissue culture media, microbiological media, buffers, and solutions White Plain, 0.2µm 100/pk 13mm White Plain, 0.2µm 100/pk 25mm White Plain, 0.2µm 100/pk 47mm White Plain, 0.2µm 50/pk 90mm White Plain, 0.45µm 100/pk 13mm White Plain, 0.45µm 100/pk 25mm White Plain, 0.45µm 100/pk 47mm White Plain, 0.45µm 50/pk 90mm White Plain, 0.8µm 100/pk 47mm White Plain, 1.0µm 47mm 100/pk RC µm, 110mm 25/pk RC µm, 142mm 25/pk RC58 0,2µm, 47mm 100/pk RC58 0,2µm, 50mm 100/pk RC58 0,2µm, 100mm 25/pk RC58 0.2µm, 5/pk 300x600mm RC60 1µm, 47mm 100/pk RC60 1µm, 50mm 100/pk PTFE Membranes Whatman PTFE membranes are chemically stable and intert. They are suitable for applications involving aggressive organic solvents, strong acids and alkalis. PTFE membranes are particularly suitable for preparing samples for HPLC analysis. The hydrophobic nature of the membrane also has applications for air and gas sterlization. The membrane is laminated onto a non woven polypropylene support web for improved strength and handling and can be used at temperatures upto 120ºC. Applications One of the major applications for the PTFE membrane is the clarification of corrosives, solvents, and aggressive fluids. This includes the important requirement in HPLC analysis for sample filtration where any solid particles can cause permanent damage to the column. The 0.5 μm pore size is normally used. Air and gas sterilization make use of the hydrophobic characteristics of PTF E membranes and their ability to stop aqueous aerosols. Usual pore sizes are 0.2 μm and 0.5 μm. Sterile venting of vacuum manifolds, fermentation vessels, and sterile filtrate tanks and containers utilize PTF E 0.2 μm membranes. Regenerated Cellulose Membranes Regenerated cellulose is a hydrophilic membrane with a very good wet strength. It is also extremely chemically resistant, suitable for both aqueous & organic solutions. Since it is manufactured without wetting agents & due to its excellent compatibility with solvents, it is most commonly used to filter HPLC solvents RC µm, 25mm 100/pk RC µm, 47mm 100/pk RC µm, 50mm 100/pk RC µm, 100mm 25/pk 36

37 Filter Paper & Membranes Membranes WTP and TE Membrane Ranges WTP membranes use a polypropylene grid as the support material whereas the TE range uses a randomly arranged polypropylene support material mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 50/pk mm 50/pk mm 50/pk mm 50/pk mm 50/pk mm 50/pk mm 50/pk mm 50/pk mm 50/pk mm 50/pk mm 50/pk mm 50/pk mm 25/pk mm 25/pk PM 2.5 Air Monitoring Membrane A high-purity, thin PTF E membrane in a sequentially numbered chemically resistant polypropylene support ring for PM 2.5 ambient air monitoring. Whatman PM 2.5 membranes have low tare mass for accurate gravimetric determinations. The thermally stable design eliminates curling, keeps the membrane flat, and makes the filter robot-friendly. The PM 2.5 PTF E membranes are manufactured under clean room conditions. These chemically resistant, low chemical background filters permit sensitive, interference-free determinations. No glues or adhesives are used in making these 46.2 mm diameter products. Whatman hereby states that every manufacturing lot that is offered for sale, and is identified for use with the PM 2.5 reference method, conforms to EPA acceptance criteria mm 50/pk Membranes for Biology and Microbiology Cellulose Acetate Membranes Whatman cellulose acetate membranes are made from pure cellulose acetate, making them suitable for biological and clinical analysis, sterility tests, and scintillation measurements. Cellulose acetate membrane filters exhibit very low protein binding capacity. They are hydrophilic, making them suitable for aqueous and alcoholic media mm 100/pk mm 100/pk mm 100/pk mm 50/pk mm 25/pk mm 25/pk mm 100/pk mm 5/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 50/pk mm 25/pk mm 50/pk mm 100/pk mm 100/pk White Plain 0.45µm 25mm 100/pk White Plain 0.45µm 47mm 100/pk White Plain 0.2µm 47mm 100/pk Cellulose Nitrate Membranes Recommended for the majority of routine applications, this membrane is manufactured under strictly controlled conditionas. Whatman nitrocellulose membranes are known for higher strength and flexibility, low extractibles and narrow pore size distribution. Features and Benefits Narrow pore size distribution for improved surface capture and analysis Low levels of extractables to ensure sample integrity 37

38 Filter Paper & Membranes Membranes Applications Sample preparation Microbiological studies Filtration of aqueous solutions Black Gridded, Sterile 0.45µm 100/pk 47mm Filter Types White Plain Filters This is the standard membrane filter for the majority of laboratory applications involving particles and cells in the range of 0.1 μm to 12.0 μm. The residue after filtration is found to be almost completely on the surface of the membrane and allows physical recovery of deposits and microscopic examination. Gridded Filters Gridded filters make it easier to count particles, microorganisms and colonies. If a gridded membrane is required, please see Mixed Cellulose Ester Membranes µm 25mm 100/pk µm 47mm 100/pk µm 13mm 100/pk µm 25mm 100/pk µm 47mm 100/pk µm 90mm 25/pk µm 142mm 25/pk µm 13mm 100/pk µm 25mm 100/pk µm 47mm 100/pk µm 50mm 100/pk µm 90mm 25/pk µm 142mm 25/pk µm 47mm 100/pk µm 47mm 100/pk µm 25mm 100/pk WCN WH 37MM 0.8uM 100/pk µm 47mm 100/pk µm 90mm 25/pk µm 25mm 100/pk µm 47mm 100/pk µm 50mm 100/pk µm 142mm 25/pk µm 25mm 100/pk µm 47mm 100/pk µm 25mm 100/pk µm 47mm 100/pk µm 90mm 25/pk µm, 47mm 100/pk µm, 50mm 100/pk µm, 25mm 100/pk µm, 47mm 100/pk µm, 50mm 100/pk µm, 25mm 100/pk µm, 47mm 100/pk µm, 50mm 100/pk µm, 25mm 100/pk µm, 47mm 100/pk µm, 50mm 100/pk µm, sterile 47mm 100/pk µm with hydrophobic rim, 100/pk 50mm µm, 47mm 100/pk µm, 50mm 100/pk µm 47mm 100/pk µm 47mm 100/pk µm 47mm 100/pk Sterile without pad 0.45µm 100/pk 47mm Sterile 0.45µm 47mm 200/pk NC µm, 25mm 100/pk NC µm, 47mm 100/pk NC µm, 50mm 100/pk NC µm, 90mm 50/pk NC45.45µm, 100mm 50/pk NC µm, 110mm 50/pk NC µm 142mm 25/pk NC µm, sterile 47mm 100/pk NC20 0.2µm, 47mm 100/pk NC20 0.2µm, 50mm 100/pk Polyamide Membranes Whatman polyamide membranes are made from pure polyamide, making them the universal filter for clarification and 38

39 Filter Paper & Membranes Mixed Cellulose Ester Membranes sterile filtration. Polyamide membrane filters are mechanically very strong and exhibit excellent wet strength and dry strength. They are hydrophilic, making them suitable for aqueous and organic solutions. The membrane filters can be used up to 135ºC. Many microbiological techniques include colony counting after incubation as the standard method of quantification. Whatman gridded filters have clearly defined grid lines spaced at 3.1 or 5 mm intervals. The special ink used is nontoxic and completely free from bacterial growth inhibitors. Whatman black mixed cellulose esters are available plain for automatic colony counting applications, as well as gridded to assist in manual counting procedures. Black membranes provide contrast between residue or cell colors and the filter without having to counter-stain the membrane. Polyamide membrane (0.45 μm, Type NL 17) electronic micrograph (magnification 1000 ) µm, 25mm 100/pk µm, 47mm 100/pk µm, 50mm 100/pk µm, 25mm 100/pk µm, 47mm 100/pk µm, 50mm 100/pk Polyethersulfone (PES) Membranes Whatman TM Polyether Sulfone (PES) membranes are hydrophilic, low protein binding, and stable in alkaline ph. Available in a 0.8 μm pore size, the PES membrane is recommended for aqueous applications and for biological samples. The Whatman TM PES membrane has a smooth surface that allows for easy enumeration of artifacts PES 47mm 0.8µm 100/pk Sterile Filters For those laboratories preferring to use membranes sterilized by autoclaving for microbiological work, Whatman provides black gridded membranes in packs with pads ready for laboratory autoclaving. Features and Benefits Sterile options available for critical applications Excellent contrast for easier particle detection Grids are nontoxic and do not inhibit bacterial growth, ensuring sample integrity Black plain and black gridded membranes have a mix of cellulose nitrate and cellulose acetate The membrane offers a high degree of internal surface area for greater adsorption of product Higher dirt loading capacity Biologically inert with good thermal stability No surfactants to contaminate samples Uniform microporous structure of membrane gives high flow rates Thermally stable Polypropylene Membranes µm 47mm 100/pk Mixed Cellulose Ester Membranes Whatman mixed cellulose ester membranes are composed of cellulose acetate and cellulose nitrate. These membranes are characterized by a smoother and more uniform surface than pure nitrocellulose filters. Also, the color contrast provided by the filter surface facilitates particle detection and minimizes eye fatigue. Mixed Cellulose Ester Membranes Circles, 0.2µm, 25mm 100/pk Circles, 0.2µm, 47mm 100/pk Circles, 0.2µm, 50mm 100/pk Circles, 0.2µm, 100mm 50/pk Circles, 0.2µm, 110mm 50/pk Circles, 0.2µm, 142mm 25/pk Circles, 0.2µm, 47mm 100/pk Circles, 0.2µm, 50mm 100/pk Circles, 0.45µm, 25mm 100/pk 39

40 Filter Paper & Membranes Mixed Cellulose Ester Membranes Circles, 0.45µm, 47mm 100/pk Circles, 0.45µm, 50mm 100/pk Circles, 0.45µm, 90mm 50/pk Circles, 0.45µm, 100mm 50/pk Circles, 0.45µm, 110mm 50/pk Circles, 0.45µm, 142mm 25/pk Circles, 0.45µm, 47mm 100/pk Circles, 0.45µm, 50mm 100/pk Circles, 0.6µm, 25mm 100/pk Circles, 0.6µm, 47mm 100/pk Circles, 0.6µm, 50mm 100/pk Circles, 0.8µm, 25mm 100/pk Circles, 0.8µm, 37mm 100/pk Circles, 0.8µm, 47mm 100/pk Circles, 0.8µm, 50mm 100/pk Circles, 0.8µm, 100mm 50/pk Circles, 1.2µm, 25mm 100/pk Circles, 1.2µm, 47mm 100/pk Circles, 1.2µm, 50mm 100/pk Circles, 1.2µm, 100mm 50/pk ST 1.2uM 50MM Circles white, 0.2µm, 100/pk 3.1 black grid, 47mm Circles white, 0.2µm, 3.1 black 100/pk grid, sterile, 50mm Circles, 0.45µm, 100/pk 3.1 black grid, 47mm Circles, 0.45µm, 3.1 black grid, 100/pk 50mm Circles white, 0.45µm, 3.1 black 100/pk grid, 47mm Circles white, 0.45µm, 3.1 black 100/pk grid, 47mm Circles white, 0.45µm, 3.1 black 1000/pk grid, 47mm Circles white, 0.45µm, 3.1 black 100/pk grid, 50mm Circles green, 0.45µm, 3.1 black 100/pk grid, 50mm Circles green, 0.45µm, 3.1 black 100/pk grid, 47mm Circles green, 0.45µm, 3.1 black 100/pk grid, 50mm Circles black, 0.45µm, 3.1 white grid, 50mm 100/pk Circles black, 0.45µm, 3.1 white 100/pk grid, 47mm Circles black, 0.45µm, 3.1 white 1000/pk grid, 47mm Circles black, 0.45µm, 3.1 white 100/pk grid, 50mm Circles, White Gridded, Sterile 1000/pk without pad 0.45µm 47mm Circles, White Gridded, Sterile 100/pk Autoclave pack 0.45µm 47mm Circles, Mixed Ester, White 100/pk Gridded, 0.8µm 25mm Circles black, 0.8µm, 3.1 mm 100/pk white grid, 47mm Circles white, 0.8µm, with grid, 100/pk sterile, 47mm Circles, 3µm, 25mm 100/pk Circles, 3µm, 47mm 100/pk Circles, 3µm, 50mm 100/pk Circles, 3µm, sterile, 50mm 100/pk Circles green, 0.8µm, with 3.1mm 100/pk black grid, 50mm Circles, 0.45µm, 5mm black grid, 100/pk 47mm Circles, 0.45µm, 5mm black grid, sterile, 50mm 100/pk STL Range of Membranes STL membranes are widely used in the food, beverage, pharmaceutical and cosmetic industries to determine microbial contaminants. MicroPlus and ME membranes deliver: Robustness: The high mechanical strength means that the membranes will not rupture Convenience: Sterile, individually packed, folded (STL) band of filters are housed in dispenser boxes with 100 numbered membrane filters for easy removal and safe handling with a membrane dispenser (supplied in 4 boxes each with 100 membrane filters). Single sterile (ST) packed filters are also available

41 Filter Paper & Membranes Accessories Speed and economy: The combination of high flow rate and membrane composition from standard mixed cellulose esters means that you can accomplish your processes faster without the need to reestablish standard experimental procedures Versatility: The hydrophilic membranes are specifically made for aqueous solutions Easy quantitation: All the membrane filters have a high contrast grid (3.1 mm), which allows convenient counting of the microbial colonies Microplus STL Membranes (Cellulose nitrate) Circles white, 0.45µm, 400/pk 3.1 black grid, 47mm Circles white, 0.45µm, 400/pk 3.1 black grid, 50mm Circles black, 0.45µm, 400/pk 3.1 white grid, 47mm Circles black, 0.45µm, 400/pk 3.1 white grid, 50mm Circles green, 0.45µm, 400/pk 3.1 black grid, 47mm Circles green, 0.45µm, 400/pk 3.1 black grid, 50mm Circles white, 0.45µm, 3.1 grid 100/pk black, 47mm Circles white, 0.45µm, 3.1 grid 100/pk black, 50mm Circles black, 0.45µm, 3.1 grid 100/pk white, 50mm Circles, Black Gridded, 0.45µm 47mm 100/pk ME STL membranes (mixed esters) 0.2µm Circles white, 0.2µm, 400/pk 3.1 grid black, 47mm Circles white, 0.2µm, 3.1 grid black, 50mm 400/pk 0.45µm Circles white, 0.45µm, 3.1 black 400/pk grid, 47mm Circles white, 0.45µm, 3.1 black 400/pk grid, 50mm Circles, 0.45µm, 5mm black grid, 400/pk 50mm Circles black, 0.45µm, 400/pk 3.1 white grid, 47mm Circles black, 0.45µm, 400/pk 3.1 white grid, 50mm Circles black, 0.8µm, 400/pk 3.1 mm white grid, 47mm Circles green, 0.45µm, 400/pk 3.1 black grid, 47mm Circles white, 0,45µm, 100/pk 3.1 black grid, sterile, 47mm Circles white, 0.45µm, 3.1 black grid, 47mm 400/pk 0.6µm Circles black, 0.6µm, 3.1 white 100/pk grid, 50mm Circles black, 0.6µm, 3.1 white 400/pk grid, 50mm Circles black, 0.6µm, 3.1 white 100/pk grid, 47mm Circles black, 0.6µm, 3.1 white 100/pk grid, 50mm Circles white, 0.8µm, with grid, 47mm 100/pk 0.8µm 41

42 Filter Paper & Membranes Mixed Cellulose Ester Membranes / Accessories mm 400/pk Circles green, 0.8µm, 3.1 black 400/pk grid, 50mm Circles white, 0.8µm, 3.1 black 400/pk grid, 50mm Circles white, 0.8µm, /pk mm, black grid, sterile, for Membrane-Butler, 47mm Circles black, 0.8µm, 3.1 mm white grid, 47mm 100/pk Membrane Filter Holders Whatman offers a choice of holders for use with membrane filters. Produced from borosilicate glass and available with a choice of support screen.suitable for aqueous and organic solvent filtration. The funnel seal ensures that the sample does not bypass the membrane and that particulates are retained on the surface of the membrane. The sintered glass support is recommended for filtration and biological analysis. The 304 stainless steel support screen is suitable for use with proteinaceous solutions. Applications General laboratory microfiltration Quality control and sterility testing Removal of particulates from HPLC solvents Tissue culture media filtration Membrane holder Glass support 25mm, 25ml 1/pk Glass support 47mm, 300ml 1/pk Glass support 90mm, 1000ml 1/pk Glass support 25mm, 50ml 1/pk Stainless steel 25mm, 25ml 1/pk Stainless steel 47mm, 300ml 1/pk Glass reservoir 47mm, 300ml 1/pk Stainless steel, for 25mm filters 1/pk Polysulfone, for 25mm filters 10/pk Polysulfone, for 50mm filters 1/pk FP 050/0/01 UPPERSECTION 1/pk FP 050/0/13 CONNECTOR M12 FOR 1/pk HOSE NIPPLE Polysulfone, for 50mm filters 5/pk Polysulfone, for In-line filtration 1/pk 50mm Polysulfone, for In-line filtration 50mm 5/pk 42

43 Filter Paper & Membranes Accessories Syringe Type Filter holders Available in stainless steel and polypropylene with luer fittings for use with a standard syringe. The holders are designed for the quick and easy clarification, sterilization, and removal of particulates from small volume samples, typically for HPLC applications. The holders contain PTFE gaskets and O-rings and allow the membrane to be autoclaved in place without the filter sticking to the holder. Plastic holders mm 10/pk mm 10/pk mm 8/pk Stainless steel holders mm 1/pk mm 1/pk Single place vacuum filtration apparatus MV 050 Series All MV series vacuum filtration devices are made of stainless steel, which is especially suitable for microbiological applications. The system can be used up to 200ºC, is autoclavable and can be sterilized by dry heat up to 180ºC. Technical Data - Vacuum Filtration - MV 050 Series Apparatus Selection Filter size 47/50 mm Filter volume 100 or 500 ml Filter area 12.5 cm 2 Prefilter 40 mm diameter Vacuum connection Rubber stopper Filter support Sleve (frit as accessory) Materials Selection Upper and lower parts Stainless Steel Cover Stainless Steel Frit Stainless Steel Sleeve Stainless Steel Seats PTFE and silicone Clamps Aluminium Vaccum Filtration - MV 050 Series MV 050/0/10 1/pk Rubberstopper for SF100+ WT MV 050/0/09 SILICONE 1/pk SEALING FOR LID * MV050A/0 stainless 1/pk steel, 500ml, 47/50mm MV 050/1/05 PTFE GASKET 50x43x0.5mm 1/pk Applications Microbiology (e.g., Escherichia coli detection), biochemistry, hydrobiology Drinks (e.g., cold sludge in beer), foodstuffs (e.g., ice cream), pharmaceuticals, cosmetics, water, wastewater Residue analysis, precipitate analysis, contamination tests 43

44 Filter Paper & Membranes Accessories MV050/0 stainless steel, 500ml, 47/50mm MV050/0/01 Funnel complete for MV050/0, 500ml MV050/0/03 Sieve for MV050-series GV Series (Glass) 1/pk 1/pk 1/pk GV025/0 Vacuum filter holder, Glass, 60ml, 25mm, glass frit GV025/2 Vacuum filter holder, 60ml, 25mm, glass frit, 250ml Erlenmeyer GV050/0 Vacuum filter holder, Glass, 250ml, 47/50mm, glass frit GV050/1 Vacuum filter holder, glass, 250ml, 47/50mm, support screen GV050/2 Vacuum filter holder, glass, 250ml, 47/50mm, glass frit, 1000ml Erlenmeyer GV050/3 Vacuum filter holder, glass, 250ml, 47/50mm, sieve, 1000ml Erlenmeyer GV100/0 Vacuum filter holder, glass, 500ml, 100mm, glass frit GV100/1 Vacuum filter holder, glass, 500ml, 100mm, sieve GV 100/1/03 SUPPORTSCREEN TEFLON GV 100/1/05 PTFE P TFECENTRING W/ SUPPORT GV100/0/01 Funnel 500ml, for GV100-series GV100/0/02 Lower part, for GV100-series 1/pk 1/pk 1/pk 1/pk 1/pk 1/pk 1/pk 1/pk 1/pk 1/pk 1/pk 1/pk GV 100/0/03 GLASS FRIT 1/pk GV 100/0/05 PTFECENTRING 1/pk RING FOR FRIT GV 100/0/06 LOCKING 1/pk CLAMPW/ SILICON-O-RING GV050/2/02 Lower part, 1/pk glass, NS29, for GV050/ GV050/2/08 Erlenmeyer flask 1/pk NS 29, 1000 ml GV050/2/12 Lower part, 1/pk glass, NS45, for GV050/ GV050/2/13 Erlenmeyer flask 1/pk NS 45, 1000 ml GV050/1/03 support screen 1/pk for GV050 sieries GV050/0/01 Funnel 250ml 1/pk without cap, for GV050- series GV050/0/02 Lower part for 1/pk GV050/0 1/pk GV050/0/03 Glass frit for 1/pk GV050-series GV050/0/05 PTFE center ring 1/pk for GV050-series, to be used with GV 050/0/12 RUBBERCAP 1/pk GV 025/2/02 SOCKET HOSE 1/pk CONNECTOR RD GV 025/2/08 ERLENMEYER 1/pk FLASK250ML GV 025/0/01 FUNNEL 60 ML 1/pk GV 025/0/03 GLASS FRIT1/PK 1/pk GV 025/0/05 PTFE CENTRING 1/pk RING FOR GV 025/0/06 SPRING-ACTION 1/pk CLAMP GV/MV050/0/06 Clamp for 1/pk GV050- and MV050-series GV050/0/10 1/pk RUBBERSTOPPER FOR SF GV100/0/10 RUBBERSTOPPER SF 100 1/pk Multiple Vacuum Filtration Apparatus AS 300 and 600 Series The stainless steel manifold for 3 or 6 filtration units is fitted with stainless steel units. The apparatus can be autoclaved and sterilized by dry heat at up to 180ºC. Suitable only for vacuum operation. If flushing tubes are used, do not exceed 1.3 bar (300 44

45 Filter Paper & Membranes Accessories mbar over-pressure). Applications Microbiological quality control Residue analyses Serial filtration carried out rapidly and easily with a common drainage outlet Technical Data - AS 300 and 600 Series - Multiple Vacuum Filtration Apparatus Apparatus Selection Filter size 47/50 mm Filter volume 100 or 500 ml Manifold 3 or 6 stopcocks and lower parts for individual choice of filter units Filter support Vacuum connection Sleve (frit as accessory) Tubing razzle 9 mm (inside diameter) Multiple filtration apparatus complete and ready for use. Filters and prefilters sold separately ml, 47/50mm, 1/pk ml, 47/50mm 1/pk ml, 47/50mm, 1/pk AS003/0/05 Cap with hose 1/pk nipple for filter bottles ml, 47/50mm 1/pk ml, 47/50mm 1/pk A100ml, 47/50mm 1/pk Place Manifold, Stainless 1/pk Steel Place Manifold, Stainless Steel 1/pk Microbiology Manifold & Accessories MBS I plastic funnel of 20/pk PP, auto clavable,100 ml Steel frit with ring for 1/pk AS MBS I plastic funnel of 20/pk PP, auto clavable, 350 ml Autoclaving bags for MBS I plastic funnels 20/pk Dispenser for 100 ml and 350 ml funnels for MBS AS220 2-place Vacuum filtration manifold for MBS Membrane-Butler- Manual Butler for dispensing filtration membranes Pressure Filtration Devices 1/pk 1/pk 1/pk Pressure filtration devices with a sample loading cylinder are suitable for batch filtration of samples from 20 ml, while devices without infusion cylinders are connected inline and are suitable for larger volumes of several liters. Filtration of liquids and gases is possible, including sterile filtration of serums or the clear filtration of media that are difficult to filter, especially those that are highly viscous.membranes, paper or glass fiber filter discs can be used. Cleaning and changing of filters is completed in a few steps. All units are equipped with pressure resistant filter supports. Highquality silicone or PTFE O-rings seal the systems. Please ensure you only use intact seals for safety reasons. PTFE versions are available, in addition to stainless steel devices, for use with corrosive media MD 050/0/03 SUPPORT 1/pk SCREEN MD 050/0/04 SUPPORT 1/pk SIEVE TEFLONIZED MD050/4 Pressure filtration 1/pk apparatus, stainless steel, 200ml, 50mm MD142/5 Pressure filtration 1/pk apparatus, stainless steel, 142mm MD142/5/3 Pressure 1/pk filtration apparatus, stainless steel, infusion vessel 2200ml, 142mm MD142/7/3 Pressure filter 1/pk holder, PTFE, infusion vessel 1500ml, 142mm MD142/2/03 Sieve (teflonisied) for MD142/5- and MD142/6-series 1/pk 45

46 Filter Paper & Membranes Accessories MD 142/2/04 SUPPOR TSIEVE TEFLONIZED MD 142/0/66 SILICONE-O- RING 134X5MM MD 050/0/11 CONNECTOR Z4 R3/8" on both sides MD 050/0/12 CONNECTOR Z1 quick release MD 050/0/13 CONNECTOR Z2 R3/8" with hose nipple NW MD 050/0/18 CONNECTOR Z3 R3/8" with hose nipple NW 7 1/pk 1/pk 1/pk 1/pk 1/pk 1/pk Membrane forceps Cat. No. Qty /pk Vacuum pump and accessories VP 003 1/pk ML 050/0 Filtration apparatus, stainless steel, for In-line filtration 1/pk ML 050/0/03 Steel frit with ring 1/pk SV 006 Vacuum tubing, 1/pk 1 meter length SF 100 Suction flask, 1000ml 1/pk with tubing nozzle WT 100 Witt's flask, 1000ml 1/pk with tubing nozzle PZ 001 Tweezers, stainless steel 1/pk 46

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48 48 Filter Paper & Membranes Diagnostic Membranes

49 Filter Paper & Membranes Diagnostic Membranes Absorption Pads 49

50 Filter Paper & Membranes Diagnostic Membranes Flow-Thru Immunoassays 50

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52 Specimen Collection Cards & Devices Whatman 903 Specimen Collection Paper is globally known for its high quality for collecting blood samples and has an international reputation as the marketleading paper for purity and reproducibility. Whatman FTA Elute Cards are used to collect DNA from virtually any source. DNA can then be amplified for a variety of downstream applications. Whatman FTA - Forensics of the future 52

53 Specimen Collection Cards 903 TM Population Screening (Guthrie cards) Whatman offers FDA and CDC approved 903TM specimen collection paper widely used for body fluid collection, transport, analysis and archiving. Applications: Population screening Screening the population for: HIV HCV Hemoglobin AIC testing Tuberculosis More recently, many programs are adding less frequently occurring disorders to their panel of assays, including MCAD, cystic fibrosis and a range of amino acid disorders. A Protein Saver Snap pk/ Proteinsaver card pk/ x 297mm pk/ x 57cm pk/ x 58cm pk/ Foil Barrier Reusable pk/100 Bags Biohazard Labels pk/ Protein Saver Card pk/ Dry Rack Without Velcro pk/ Dry Rack Without Velcro pk/ Plastic Zip Lock Bags pk/ Desiccant Packs pk/ Glassine Envelopes pk/100 FTA Technology Collect, Transport, Archive, and Isolate Nucleic Acids all at room temperature FTA and FTA Elute cards utilize patented Whatman FTA technology that simplifies the handling and processing of nucleic acids. FTA Cards contain chemicals that lyse cells, denature proteins, and protect nucleic acids from nucleases, oxidative, and UV damage. FTA Cards rapidly inactivate pathogens, and prevent the growth of bacteria and other microorganisms. FTA Cards used with virtually any sample type : Blood Cultured Cells Buccal Cells Plant Material Bacteria Plasmids Microorganisms Solid Tissue WB Fta Classic Card pk/100 WB Indicating FTA Classic pk/100 WB FTA Classic Card pk/25 WB Indicating FTA Classic pk/25 WB FTA Genecard pk/25 WB FTA Micro Card pk/25 WB Indicating FTA Micro pk/25 WB FTA Plantsaver pk/25 WB FTA Genecard pk/100 WB FTA micro card pk/100 WB Indicating FTA Micro pk/100 WB FTA Plantsaver pk/100 WB Clonesaver Card pk/5 53

54 Specimen Collection Cards FTA Cards WB FTA Minicard - 2 SPOT pk/100 WB Indicating FTA Minicard pk/100 WB FTA Minicard - 2 SPOT pk/25 WB Indicating FTA Minicard pk/25 Use FTA for a wide range of applications : Forensics Transgenic Identification Plasmid Screening Food and Agriculture Testing Drug Discovery Genomics STR Analysis Animal Identification Molecular Diagnostics Whole Genome Amplification Molecular Biology FTA Plant Kit Includes: 20 FTA PlantSaver Cards, 2.0 mm Uni-Core Punch with cutting mat, 50 ml FTA Purification Reagent, 1 pair of nitrile gloves, 1 cutting mat and round bottom test tube for sample crushing, instructions. WB FTA Plant Kit pk/1 Clonesaver Card Whatman offers a patented technology to collect, store, backup and process clone samples. This revolutionary FTA technology is available in a 96 well card Format for High-Through put Applications. Designed for the collection and storage of plasmid and BAC DNA from bacterial clones. WB Clonesaver Card pk/1 FTA Kit Includes: 25 FTA Micro Cards, 50 ml FTA Purification Reagent, 2 Harris Uni-Core Punches with cutting mat, instructions. WB FTA KIT pk/1 FTA Kit for blood Each kit contains FTA Minicard, Multi barrier pouch, Desiccants, Lancet, Gloves & Instructions for use. WB FTA Kit for buccal and colourless fluids Each kit contains Indicating FTA Minicard, Multi barrier pouch, Desiccants, Foam tipped applicator, Gloves & Instructions for use. WB Easicollect Buccal sample collection device Buccal sample collection device with unique foam surface for collection of maximum number of cells. Designed for simple and efficient transfer of cells in to a built-in FTA card. WB Genesys Buccal Device pk/50 FTA Elute Cards Long-term DNA storage at room temperature combined with easy elution for downstream applications from a single sample. FTA Elute Cards utilize patented Whatman FTA technology that simplifies the handling and processing of nucleic acids. DNA is eluted easily, providing you with DNA in solution for your amplification needs. Applications: Biobanking Genotyping Molecular Diagnostics Transgenics Pharmacogenics Rapid DNA isolation Genetic identification PCR WB FTA Elute Micro Card pk/25 WB FTA Elute Micro Card pk/100 WB Indicating FTA Elute Micro Card pk/100 54

55 Specimen Collection Cards FTA Accessories WB Indicating FTA Elute Micro Card pk/25 Collect and Isolate Samples Quickly and Easily Blood Sample Collection and Isolation of DNA Template WB Harris Micro Punch pk/1 1.2 mm Tip WB Harris Micro Punch pk/1 2.0 mm WB Harris Micro Punch pk/1 2.0 mm Tip WB Bloodstain Cards pk/100 WB mm Replacement Plunger pk/1 WB mm Replacement Plunger pk/1 WB Fta Gene Card Carrier pk/20 WB Harris Punch 3.0 mm 1/pk Replacement Plunger WB Harris Punch 3.0Mm Replacement Tip 1/pk FTA Accessories For convenient collection, storage, processing and shipping FTA and FTA Elute Cards. WB FTA Purification Reagent 500ml pk/1 WB HARRISUNI-CORE 1.25MM pk/4 PUNCH WB HARRISUNI-CORE 2.0 MM PUNCH pk/4 WB HARRISUNI-CORE 3.0 MM PUNCH pk/4 WB HARRISUNI-CORE 6.0 MM PUNCH pk/4 WB Replacement Cutting Mat pk/1 WB Harris Micro Punch 1.2 mm pk/1 WB Sterile Foam Tipped pk/100 Swab WB Spot Holder pk/1 WB Omni Swab Pack pk/100 WB Small (8 x 7 cm) pk/100 WB Large (9 x 15 cm) pk/100 WB Clonesaver Resealable pk/50 Pouch WB DESSICANT with indicator pk/

56 Specimen Collection Cards DMPK Cards DMPK cards (Drug Metabolism & pharmaco-kinetics) An easy & convenient way of collecting, shipping & storing a wide variety of samples including whole blood, plasma & serum. Quantification can be conveniently done by using LC-MS / MS Assay. Ethical: Reduce number of animals used (up to 50%) Elimination/reduction of rodent warming Technical/Clinical Supports juvenile testing/paediatric testing as lower volumes are required Compound/metabolite stabilized on card Serial sampling from one rodent (no composites from several individuals) Finger prick collection of clinical samples Cost savings Reduction in animals used Reduced shipping costs (room temperature and nonhazardous) Reduced storage costs (room temperature) Less test compound consumed in Discovery phase Simplified process (time saving) WB FTA DMPK-A IND Card 100/pack WB FTA DMPK-B IND Card 100/pack WB FTA DMPK-C IND Card 100/pack WB FTA DMPK IND Starter Pack 1/pack Features and Benefits Easy visualization of samples. Fast and easy collection of biofluids. Convenient room temperature storage and shipping. WB FTA DMPK A 100/pack WB FTA DMPK B 100/pack WB FTA DMPK C 100/pack WB FTA DMPK Starter Pack 1/pack Indicating FTATM DMPK Cards Drug studies involve the analysis of colorless biological fluids in order to support toxicological and pharmacokinetic/ pharmacodynamic (PK/PD) studies. Whatman Indicating FTATM DMPK Cards combine the advantages of the traditional FTA cards with the ability to clearly visualize colorless samples both immediately after application and after drying. Whatman Indicating FTA -DMPK Cards are available as FTA DMPK-A, DMPK-B, and DMPK-C cards and also as a starter pack. *Each pack consists of 15 x FTA DMPK-A IND Cards, 15 x FTA DMPK-B IND Cards and 15 x FTA DMPK-C IND Cards sealed in an aluminum foil pouch and packaged in a cardboard box. Also included is a Harris Uni-Core Punch (3.0mm) and cutting mat. 56

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58 GD/X Syringe Filters The GD/X range is specifically designed for high particulate loaded samples. Constructed of a pigment-free polypropylene housing with a prefiltration stack of Whatman GMF 150 and GF/F glass microfiber media, these filters eliminate sample contamination and allow you to filter even the most difficult samples with less hand pressure. GD/X syringe filters can process three to seven times more sample volume than standard syringe filters. Mini-UniPrep syringeless filters: Whatman Mini-UniPrep syringeless filters provide a faster and easier way to remove particulates from samples being prepared for HPLC and UHPLC analysis. Mini-UniPrep lets you prepare samples in one-third the time required by other methods. Mini-UniPrep is a preassembled filtration device consisting of a 0.4 ml capacity chamber and a plunger. The device can be used either manually or with a compressor unit. Carbon Cap: A capsule filter that is filled with high-purity, highefficiency, acid washed, granular-activated carbon and a pleated HEPA filter. It is made specially to meet the requirements for continuous column percolation purification processes. This filter capsule is suitable for adsorption of organics from air and removal of color, organics, and chlorine from water. 58

59 Filtration Devices Whatman disposable filtration devices are designed to enable filtration of many types of samples. They are available in a wide variety of filter choices with a polypropylene or polycarbonate housing and utilize the most advanced construction methods and design features. This level of engineering provides for the finest disposable filtration devices possible. 59

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64 Filtration Devices Syringe filters GD/X Syringe Filters The GD/X range is specifically designed for high particulate loaded samples. Constructed of a pigment-free polypropylene housing with a prefiltration stack of Whatman GMF150 (graded density) and GF/F glass microfiber media, these filters eliminate sample contamination and allow you to filter even the most difficult samples with less hand pressure. GD/X syringe filters can process three to seven times more sample volume than standard syringe filters. GMF 150 and GF/F are produced from 100% borosilicate glass microfiber. Benefits Eliminates sample contamination Requires less hand pressure, even with the most difficult samples Processes three to seven times more sample volume 13 mm GD/X Syringe Filters GD/x Nylon µm 150/pk µm 150/pk µm 1500/pk µm 1500/pk GD/X PVDF µm 150/pk µm 150/pk µm 150/pk µm 1500/pk GD/X PTFE a µm 150/pk µm 1500/pk µm 1500/pk GD/X PES µm 150/pk µm 150/pk GD/X Cellulose Acetate µm 150/pk µm 150/pk GD/X Polypropylene µm 150/pk µm 150/pk GD/X Glass Fibre µm, GF/A 150/pk µm, GF/B 150/pk µm, GF/C 150/pk µm, GF/D 150/pk µm, GF/F 150/pk µm 150/pk 64

65 Filteration Devices Syringe Filters 25 mm GD/X Syringe Filters GD/X Nylon µm 150/pk µm 150/pk µm 150/pk µm 1500/pk µm 1500/pk µm 1500/pk µm 150/pk GD/X PVDF µm 150/pk µm 150/pk µm 1500/pk µm 1500/pk GD/X 25 Syringe Filter, 0.45µm Cellulose Acetate GD/X Applications Dissolution testing Content uniformity Concentration analysis Routine sample preparation Food analysis Environmental samples Composite assay 1500/pk GD/X PTFE µm 150/pk µm 150/pk µm 1500/pk µm 1500/pk GD/X Polypropylene µm 150/pk µm 150/pk µm 1500/pk GD/X PES GD/X 25 Syringe Filter, 150/pk 0.2µm Polysulphone GD/X 25 Syringe Filter, 150/pk 0.45µm Polysulphone GD/X 25 Syringe Filter, 1500/pk 0.2µm Polyether sulphone GD/X 25 Syringe Filter, 1500/pk 0.45µm Polyether sulphone GD/X 25 Syringe Filter, 150/pk 0.2µm Cellulose Acetate GD/X 25 Syringe Filter, 0.45µm Cellulose Acetate 150/pk GD/X Regenerated Cellulose µm 150/pk µm 1500/pk GD/X Cellulose Acetate µm 150/pk µm 150/pk µm 1500/pk GD/X Glass Fibre µm GF/A 150/pk µm GF/A 1500/pk 65

66 Filteration Devices Syringe Filters µm GF/B 150/pk µm GF/C 150/pk µm GF/D 150/pk µm GF/F 150/pk µm GF/F 1500/pk µm 934AH 150/pk µm GMF 150/pk µm GMF 1500/pk µm Polyethersulphone µm Polyethersulphon µm Polyethersulphone µm PVDF µm PVDF µm Cellulose Acetate µm Cellulose Acetate µm Glass Microfibre GD/X Sterile Syringe filters Cat. No. Description µm Polyethersulphon GD/XP Syringe Filters Whatman GD/XP disposable syringe filters are designed for use with samples that require inorganic ion analysis, as levels of ion extractables are minimized. They are also an alternative choice for users requiring c filter that exhibits extremely low protein binding characteristics. GD/XP syringe filters contain a two layer prefilter stack comprised of 20 urn and 5 urn polypropylene filters. The last stage of filtration is a choice of membrane, which is positioned below the prefilter stack. Applications HPLC sample preparation Trace metal analysis Sample preparation prior to determination of dissolved heavy metals GD/XP Nylon µm 150/pk µm 1500/pk 66

67 Filteration Devices Syringe Filters GD/XP PVDFa Cat. No. Pore Size mm µm µm GD/XP PES µm 150/pk µm 1500/pk GD/XP PTFE Cat. No. Pore Size mm µm GD/XP PP µm 150/pk µm 1500/pk Puradisc Syringe Filters Puradisc syringe filters are produced from pigment-free polypropylene or polycarbonate with standard inlet (female luer lock) and outlet (male luer) connections (unless otherwise stated). They are used for the quick, efficient filtration of samples up to 100 ml volume. Features and Benefits Optional sterile, medical-grade blister pack Tube-tip format (optional) for accurate dispensing into a micro-vial Choice of membrane or glass microfiber filter media Choice of filter sizes (4,13 or 30 mm) to minimize sample loss Wide range of membranes Applications HPLC samples containing low solid content filtration will improve column life CE [Capillary Electrophoresis) samples filtration will eliminate spurious peaks Sterile filtration of low volume samples UV/Vis samples filter directly into cuvette using tube tip Refractometry filter samples to prevent damage to instrument optics and improve accuracy of results Minimizing nonspecific binding to membrane (due to small membrane size] Puradisc 4mm Syringe Filters Puradisc 4 Nylon μm 100/pk μm 100/pk μm 500/pk μm 500/pk μm 50/pk Puradisc 4 PVDF μm 50/pk μm 100/pk μm 100/pk μm 50/pk μm 500/pk μm 500/pk Puradisc 4 PVDF Sterile μm 50/pk Puradisc 4 PTFE μm 500/pk μm 500/pk μm 100/pk μm 100/pk 67

68 Filteration Devices Syringe Filters Puradisc 13mm Syringe Filters Puradisc 13 Nylon μm 100/pk μm 100/pk μm 100/pk μm 2000/pk μm 2000/pk μm 500/pk μm 500/pk Puradisc 13 Nylon Sterile μm 50/pk μm 50/pk Puradisc 13 PVDF μm 2000/pk μm 2000/pk μm 50/pk μm 50/pk μm 50/pk μm 100/pk μm 100/pk Puradisc 13 PVDF Sterile μm 50/pk μm 50/pk μm 500/pk μm 500/pk Puradisc 13 PTFE μm 2000/pk μm 2000/pk μm 50/pk μm 50/pk μm 500/pk μm 500/pk μm 100/pk μm 100/pk μm 100/pk μm 100/pk μm 100/pk Puradisc 13 PES μm 500/pk μm 100/pk μm 100/pk Puradisc 13 PES Sterile μm 50/pk μm 50/pk Puradisc 13 Polypropylene μm 500/pk μm 500/pk μm 100/pk μm 100/pk Puradisc 13 Glass Microfiber µm GMF 500/pk µm GF/F 100/pk µm GF/B 100/pk µm GF/C 100/pk µm GF/D 100/pk µm 934 AH 100/pk µm GF/A 100/pk μm GF/A 500/pk Puradisc 13 Cellulose Acetate μm 2000/pk μm 100/pk 68

69 Filteration Devices Syringe Filters Puradisc 25 mm Syringe Filters Puradisc 25 Nylon μm 50/pk μm 50/pk μm 50/pk μm 200/pk μm 200/pk μm 200/pk μm 500/pk μm 1000/pk μm 1000/pk μm 1000/pk µm 500/pk Puradisc 25 PTFE μm 50/pk μm 50/pk μm 50/pk μm 50/pk μm 200/pk μm 200/pk μm 1000/pk μm 1000/pk μm 1000/pk μm 1000/pk Puradisc 25 PES μm 200/pk μm 200/pk μm 200/pk μm 1000/pk μm 1000/pk µm 1000/pk μm 1000/pk μm 1000/pk Puradisc 25 PVDF µm 50/pk µm 50/pk µm 200/pk µm 200/pk µm 1000/pk Puradisc 25 GD µm 100/pk µm 100/pk Puradisc 25 PES Sterile μm 50/pk μm 50/pk μm 50/pk μm 1000/pk μm 1000/pk Puradisc 25 Polypropylene μm 50/pk μm 200/pk μm 1000/pk Puradisc 25 Depth Polypropylene µm 50/pk µm 200/pk µm 1000/pk 69

70 Filteration Devices Syringe Filters Puradisc 25 Glass Microfibre µm, GF/F 50/pk µm, GF/F 200/pk μm, GD1 100/pk μm, GD2 100/pk µm, GF/F 1000/pk μm, GD1 1000/pk Puradisc FP 30 Cellulose acetate µm 500/pk µm 500/pk µm 50/pk µm 100/pk µm 500/pk µm 50/pk µm 100/pk µm 50/pk µm 500/pk µm 50/pk µm 500/pk Puradisc FP 30 Cellulose acetate Sterile µm 50/pk µm 50/pk µm 50/pk µm 50/pk µm 50/pk µm 100/pk µm 50/pk Puradisc FP 30 Cellulose Nitrate Sterile mm 50/pk Puradisc FP 30 PTFE Sterile mm 50/pk Puradisc FP 30 Regenerated Cellulose Sterile mm 50/pk mm 50/pk mm 50/pk mm 50/pk Puradisc Aqua 30 Specifically designed for filtration of environmental samples prior to COD and DOC analysis. The membranes used in these devices are prewashed prior to assembly of the filters so as to reduce the organic carbon level µm 500/pk µm 100/pk µm 50/pk Puradisc FP 30 Cellulose Nitrate µm 500/pk 70

71 Filteration Devices Syringe Filters Anotop Syringe Filters Anotop syringe filters are a universal solution for numerous filtration applications. They can be used with most organic solvents and aqueous materials and are suitable for sample volumes up to 100 ml. The distinctive hexagonal housing is manufactured from pigment-free polypropylene to eliminate sample contamination. Anotop syringe filters contain the proprietary alumina based Anopore membrane and are supplied in three pore sizes. Glass microfiber Prefilter versions are available for difficult-tofilter samples. Anotop 10 Syringe filters μm 50/pk μm 50/pk μm 50/pk μm Sterile 50/pk μm Sterile 50/pk μm Sterile 50/pk μm 100/pk μm 200/pk Anotop Plus 10mm Syringe Filter with Microfibre prefilter μm 50/pk μm 50/pk μm 50/pk μm Sterile 50/pk μm Sterile 50/pk μm Sterile 50/pk Anotop 25 Syringe filters μm 50/pk μm 50/pk μm 50/pk μm 200/pk μm 50/pk μm Sterile 50/pk μm Sterile 50/pk μm 100/pk μm 200/pk Anotop IC µm 50/pk µm 100/pk µm 200/pk µm 200/pk µm 200/pk Anotop Plus 25mm Syringe Filter with Microfibre prefilter μm 50/pk μm 50/pk μm 50/pk μm Sterile 200/pk μm Sterile 50/pk μm Sterile 50/pk μm Sterile 50/pk 71

72 Filteration Devices Syringe Filters Uniflo Syringe Filters Uniflo syringe filters are non-sterile, single layered with 0.2 um and 0.45um available in Nylon, PTFE, PES and PVDF in higher quality packaging with blue overmold. Uniflo 13 syringe filters Uniflo 13 Nylon µm 500/PK µm 500/PK Uniflo 13 PTFE µm 500/PK µm 500/PK Uniflo 13 PVDF µm 500/PK µm 500/PK Uniflo 13 PESa µm 500/PK µm 500/PK Uniflo 25 Syringe filters Uniflo 25 Nylon µm 500/PK µm 500/PK Uniflo 25 PTFE µm 500/PK µm 500/PK Uniflo 25 PVDF µm 500/PK µm 500/PK Uniflo 25 PES µm 500/PK µm 500/PK Roby Syringe filters Roby 25mm syringe filters are specifically designed for automated sample filtration systems Glass Fibre GF92, 1µm 1000/pk Glass Fibre GF92, 1µm 200/pk Nylon, 0.45µm 200/pk Nylon with Glass Fibre 1000/pk prefilter GF92, 0.45µm Nylon with Glass Fibre 200/pk prefilter GF92, 0.45µm Regenerated Cellulose, 1000/pk 0.45µm Regenerated Cellulose, 200/pk 0.45µm Regenerated Cellulose with 1000/pk Glass Fibre prefilter GF92, 0.45µm Regenerated Cellulose with 200/pk Glass Fibre prefilter GF92, 0.45µm Cellulose Acetate with Glass 200/pk Fibre prefilter GF92, 0.45µm Glass Fibre GF55, 0,7µm 200/pk Glass Fibre GF55, 0,7µm 1000/pk Roby Automated Filter 25mm, Filter Validation Kit 1/pk Celtron Celtron 30/0,45 RC- 100/pk GF92-TG Celtron 30/0,45 RC- GF55 100/pk 72

73 Filteration Devices Syringe Filters ReZist Syringe Filters The Whatman ReZist range of PTFE syringe filters has been specifically designed to be resistant to organic solvents. These filters are suitable for the clarification of aggressive organic solvents. ReZist 30 mm filters can also be used as a venting filter for small vessels. ReZist Syringe filters mm, PTFE, 0.2µm 50/pk sterile mm, PTFE, 0.2µm 100/pk mm, PTFE, 0.2µm 500/pk mm, PTFE, 0.45µm 100/pk mm, PTFE, 0.45µm 500/pk mm, PTFE, 1.0µm 100/pk mm, PTFE, 1.0µm 500/pk mm, PTFE, 5.0µm 100/pk mm, PTFE, 5.0µm 500/pk mm, GF /pk mm, GF /pk mm, PTFE, 0.2µm 10/pk sterile mm, PTFE, 0.2µm 50/pk mm, PTFE, 0.2µm 100/pk mm, PTFE, 0.45µm 100/pk Spartan Syringe Filters SPARTAN syringe filters ensure reproducible results from the filtration of organic or aqueous solutions for HPLC. For batchto-batch consistency, the SPARTAN range of filters is tested and certified for the absence of UV-absorbing substances at wavelengths of 210 and 254 nm with water, methanol, and acetonitrile. Benefits Versatile: Use for any application requiring a chemically resistant, hydrophilic, low protein-binding membrane Documented batch-to-batch quality and consistency ensure reproducible results 13 mm diameter with Mini-Tip outlet is ideal for filtration into very small sample bottles mm, Regenerated 100/pk Cellulose, 0.45µm, with MiniTip outlet mm, Regenerated 500/pk Cellulose, 0.45µm, with MiniTip outlet mm, Regenerated 100/pk Cellulose, 0.2µm, with MiniTip outlet mm, Regenerated 500/pk Cellulose, 0.2µm, with MiniTip outlet mm, Regenerated 100/pk Cellulose, 0.45µm mm, Regenerated 500/pk Cellulose, 0.45µm mm, Regenerated 50/pk Cellulose, 0.45µm mm, Regenerated Cellulose, 0.2µm 100/pk 73

74 Filteration Devices Syringeless Filters mm, Regenerated Cellulose, 0.2µm mm, Regenerated Cellulose, 0.2µm mm, Regenerated Cellulose, 0.2µm mm, Regenerated Cellulose, 0.45µm mm, Regenerated Cellulose, 0.45µm 500/pk 100/pk 500/pk 100/pk 500/pk Syringeless Filters Whatman syringeless filters are preassembled convenient filtration devices for removing particulates from samples. They replace syringe-coupled filtration devices with single disposable units. Whatman has made sample preparation easier, faster, and more efficient with its innovative product line of syringeless filters. Mini-UniPrep Syringeless Filters The Whatman Mini-UniPrep Syringeless Filters provide a faster and easier way to remove particulates from samples being prepared for High-Performance Liquid Chromatography (HPLC) and Ultra High-Performance Liquid Chromatography (UHPLC) analysis. In fact, Mini-UniPrep lets you prepare samples in onethird the time required by other methods. Mini-UniPrep is a preassembled filtration device consisting of a 0.4 ml capacity chamber and a plunger. The device can be used either manually or with a compressor unit. The multi compressor can process up to 6 to 8 samples at one time, further improving sample processing time and reducing the risk of hand stress. Plastic Mini-Uniprep UN203NPEAQU 0.2μm PVDF 100/pk UN203NPENYL 0.2μm Nylon 100/pk UN203NPEORG 0.2μm PTFE 100/pk UN203NPEPES 0.2μm 100/pk Polyethersulphone UN203NPEPP 0.2μm Polypropylene 100/pk UN203NPERC 0.2μm RC 100/pk UN203NPUAQU 0.45μm PVDF 100/pk UN203NPUDPP 0.45μm Depth 100/pk Polypropylene UN203NPUGMF 0.45μm Glass Microfi bre 100/pk UN203NPUNYL 0.45μm Nylon 100/pk UN203NPUORG 0.45μm PTFE 100/pk UN203NPUPES 0.45μm 100/pk Polyethersulphone UN203NPUPP 0.45μm Polypropylene 100/pk UN203NPURC 0.45μm RC 100/pk UN503NPEAQU 0.2µm PVDF 1000/pk UN503NPENYL 0.2µm Nylon 1000/pk UN503NPEORG 0.2µm PTFE 1000/pk UN503NPEPES 0.2µm 1000/pk Polyethersulphone UN503NPEPP 0.2µm Polypropylene 1000/pk 74

75 Filteration Devices Syringeless Filters UN503NPERC 0.2µm RC 1000/pk UN503NPUAQU 0.45µm PVDF 1000/pk UN503NPUDPP 0.45µm Depth Polypropylene 1000/pk UN503NPUGMF 0.45µm Glass Microfibre 1000/pk UN503NPUNYL 0.45µm Nylon 1000/pk UN503NPUORG 0.45µm PTFE 1000/pk UN503NPUPES 0.45µm Polyethersulphone 1000/pk UN503NPUPP 0.45µm Polypropylene 1000/pk UN503NPURC 0.45µm RC 1000/pk Amber colored housing UN203APEAQU 0.2μm PVDF 100/pk UN203APENYL 0.2μm Nylon 100/pk UN203APEORG 0.2μm PTFE 100/pk UN203APEPES 0.2μm Polyethersulphone 100/pk UN203APEPP 0.2μm Polypropylene 100/pk UN203APUAQU 0.45μm PVDF 100/pk UN203APUDPP UN203APUGMF 0.45μm Depth Polypropylene 0.45μm Glass Microfi bre 100/pk 100/pk UN203APUNYL 0.45μm Nylon 100/pk UN203APUORG 0.45μm PTFE 100/pk UN203APUPES 0.45μm Polyethersulphone 100/pk UN203APUPP 0.45μm Polypropylene 100/pk Slit Septa US203NPEAQU 0.2μm PVDF 100/pk US203NPENYL 0.2μm Nylon 100/pk US203NPEORG 0.2μm PTFE 100/pk US203NPEPES 0.2μm Polyethersulphone 100/pk US203NPEPP 0.2μm Polypropylene 100/pk US203NPUAQU 0.45μm PVDF 100/pk US203NPUDPP 0.45μm Depth Polypropylene 100/pk US203NPUGMF 0.45μm Glass Microfibre 100/pk US203NPUNYL 0.45μm Nylon 100/pk US203NPUORG 0.45μm PTFE 100/pk US203NPUPES 0.45μm 100/pk Polyethersulphone US203NPUPP 0.45μm Polypropylene 100/pk US503NPEAQU 0.2µm PVDF 1000/pk US503NPENYL 0.2µm Nylon 1000/pk US503NPEORG 0.2µm PTFE 1000/pk US503NPEPES 0.2µm 1000/pk Polyethersulphone US503NPEPP 0.2µm Polypropylene 1000/pk US503NPUAQU 0.45µm PVDF 1000/pk US503NPUDPP 0.45µm Depth 1000/pk Polypropylene US503NPUGMF 0.45µm Glass Microfibre 1000/pk US503NPUNYL 0.45µm Nylon 1000/pk US503NPUORG 0.45µm PTFE 1000/pk US503NPUPES 0.45µm 1000/pk Polyethersulphone US503NPUPP 0.45µm Polypropylene 1000/pk Glass Mini-UniPrep G2 GN203NPEORGSP 0.2μm 100/pk GN203NPUORG 0.45μm 100/pk GN203NPUORGSP 0.45μm 100/pk GN203NPEAQU 0.2μm 100/pk GN203NPEAQUSP 0.2μm 100/pk GN203NPUAQU 0.45μm 100/pk GN203NPUAQUSP 0.45μm 100/pk GN203NPENYLSP 0.2μm 100/pk GN203NPEPP 0.2μm 100/pk GN203NPEPPSP Standard Cap 100/pk GN203APEORGSP 0.2μm 100/pk GN203APEAQUSP 0.2μm 100/pk GS203NPEORGSP 0.2μm 100/pk GS203NPUORGSP 0.45μm 100/pk Mini-uniPrep CR Mini UniPrep Compressor, 6 1/pk position UN PP WISP48 Adapter 150/pk 75

76 Filteration Devices Syringeless Filters MUPG2PWC1 76 Mini-UniPrep G2 Hand Compressor 1/pk UniPrep Syringeless Filters 1 /pk UniPrep syringeless filters are preassembled filtration devices for the filtration and storage of laboratory samples.these devices are quick and easy to use and feature a plunger,filter, and vial in one unit. They replace syringe-coupled filtration devices with single, disposable units. UniPrep devices consist of two parts: a test tube and a filterplunger. The design incorporates a prefilter and a membrane into the tip of the plunger. When the filter-plunger is pressed through the liquid placed in the test tube, positive pressure forces the filtrate up into the reservoir of the filterplunger. UniPrep devices function in a similar way to the Mini- UniPrep. However, UniPrep does not contain a septum in the cap and can be used to filter larger volumes (1 to 5 ml). Features and Benefits Integral storage vial saves time and minimizes laboratory waste Built-in glass fiber prefilter means even difficult samples are quick and easy to prepare Choice of membranes for wide sample compatibility Applications Sample preparation (e.g., prior to preparative HPLC) Difficult-to-filter samples Quick filtration of samples UN113EAQU 0.2µm PVDF 50/pk UN113ENYL 0.2µm Nylon 50/pk UN113EORG 0.2µm PTFE 50/pk UN113UAQU 0.45µm PVDF 50/pk UN113UGMF 0.45µm Glass Microfibre 50/pk UN113UNYL 0.45µm Nylon 50/pk UN113UORG 0.45µm PTFE 50/pk UN513EORG 0.2µm PTFE 1000/pk UN513UAQU 0.45µm PVDF 1000/pk UN513UNYL 0.45µm Nylon 1000/pk UN513UORG 0.45µm PTFE 1000/pk Autovial Syringeless Filters Autovial syringeless filters replace syringe-coupled filtration devices with single, disposable units. AV125UCA 0.45µm Cellulose Acetate 50/pk AV125UGMF 0.45µm Glass Microfibre 50/pk AV125UNAO 0.45µm Nylon 50/pk AV125UORG 0.45µm PTFE 50/pk AV125UPP 0.45µm Polypropylene 50/pk AV525BGMF 1.0µm GF/B 1000/pk AV525UNAO 0.45µm Nylon 1000/pk AV525UORG 0.45µm PTFE 1000/pk AV115NPEORG 5ml, 0.2µm PTFE 50/pk AV115NPUAQU 5ml, 0.45µm PVDF 50/pk AV115NPUNYL 5ml, 0.45µm Nylon 50/pk AV115NPUORG 5ml, 0.45µm PTFE 50/pk AV115UGMF 5ml, 0.45µm Glass 50/pk Microfibre AV125EAQU 12ml, 0.2µm PVDF 50/pk AV125ENAO 12ml, 0.2µm Nylon 50/pk AV125EORG 12ml, 0.2µm PTFE 50/pk AV125EPP 12ml, 0.2µm Polypropylene 50/pk AV125NPUAQU 12ml, 0.45µm PVDF with no 50/pk prefilter AV125NPUPSU 12ml, 0.45µm Polysulphone 50/pk with no prefilter AV125SAQU 12ml, Sterile 0.2µm PVDF 40/pk AV125SNAO 12ml, Sterile 0.2µm Nylon 40/pk AV125SORG 12ml, Sterile 0.2µm PTFE 40/pk AV125UAQU 12ml, 0.45µm PVDF 50/pk Polydisc Filters Whatman Polydisc 50 mm inline disc filters are designed for larger volume sample filtration in the laboratory, at a pilot plant, or in manufacturing. They are extremely versatile and cost effective. Sample volumes up to 1 liter can be filtered with one device. Polydisc devices can be used in conjunction with a syringe or connected inline via stepped hose barbs. The devices are autoclavable and sterile options are available. Polydisc AS The Polydisc AS (Aqueous Solution) family of 50 mmfilter devices features a high throughput polyethersulfonemembrane, which has low protein binding and nosurfactants, developed for use

77 Filteration Devices Polydisc Filters in the pharmaceuticalindustry. A glass microfiber prefilter extends the life ofthe membrane and effectively filters heavily contaminatedsamples. Each Polydisc AS device has a sterility cap onthe outlet and is sealed in its own medical-grade clearblister pack, radiation sterilized, and secured in aprotective shelf pack AS, 0.2μm sterile 10/pk AS, 0.45μm sterile 10/pk AS, 0.45μm 50/pk Polydisc SPF Contains a stack of filter media for the prefiltrationof serum and other hard-to-filter solutions. The glass microfiber and polyethersulfone membrane filterstack effectively filters the complex particulates found in serum samples SPF 10/pk Polydisc TF This device features a PTFE membrane, which is suitable for chemically aggressive solutions, reagents, and organic solvents. This lightweight unit is particularly suitable for protective vents and for inline filtration and isolation applications. The 1 μm device features a polypropylene prefilter for use with heavily contaminated samples μm 10/pk μm 10/pk μm 10/pk μm 10/pk Polydisc HD Excellent flow rate characteristics for filtering large volumesto 1 liter of aqueous and solvent samples.polydisc HD (HeavyDuty) is available in 5 and 10 μm retention ratings HD, 5.0μm 10/pk HD, 10.0μm 10/pk Polydisc GW Polydisc GW (Ground Water) is specifically designed forsample preparation of ground water samples for the analysisof dissolved heavy metals. It is an aqueous filter with lowbackground values for the determination of trace elements.it has everything that makes the preparation of aqueoussolutions for the analysis of dissolved heavy metals easy:a large filter surface, quartz fiber prefilter, and membrane filterin sandwich arrangement and a high dirt loading capacity.and, of course, it meets all the requirements of regulationssuch as NEN, EPA mm 20/pk mm 50/pk Applications Polydisc AS: Tissue culture media Pharmaceutical preparations Polydisc SPF: Virology, microbiology, and tissue culture laboratories Immunoassay methods Polydisc TF: Pharmaceutical: vents and inline applications Biotech: sterile vents, inline sterilization of gases Laboratory: clean or sterile gases, filtration of solvents and reagents, drying gases Polydisc HD: Large volume sample preparation Vacu -Guard TM These easy-to-use inline filter devices help to confine and isolate infectious materials in vacuum systems and protect your laboratory mm Disc 10/pk mm Disc 10/pk Vacuguard 150, 1/pk Activated Carbon Vacuguard 150, 1/pk Dessicant Vacuguard 150, Molecular Sieve 1/pk Polycap Capsule Filters Polycap AS (Aqueous Solution) is recommended for filtering aqueous solutions. 77

78 Filteration Devices Capsule Filters Polycap HD (Heavy Duty) is a well-engineered product that offers high filtration efficiency and excellent filtrate purity. Polycap SPF (Serum Prefilter) is an exceptional product that is optimized for prefiltration applications and is typically used upstream of a Polycap AS or a Polycap PES capsule for filtration of serum. Polycap TF filter devices are among the finest disposable encapsulated filters. They are designed for use with organic solvents and chemically aggressive solutions. Polycap 36 AS μm 1/pk μm 1/pk μm (with filling bell) 1/pk μm 1/pk μm 1/pk 2606T Polycap AS µm, Non-sterile, 5/pk Inlet: inch FNPT, Outlet: inch FNPT 2642 Polycap PES 36, 0.2µm 5/pk Polycap 75 AS μm 1/pk μm 1/pk μm 1/pk μm (with filling bell) 1/pk μm 1/pk μm 1/pk μm 1/pk 2706T Polycap AS 75, 0.2µm, Non-sterile, 5/pk Inlet: inch FNPT, Outlet: inch FNPT 2707NS Polycap AS 75, 0.45µm, Non-sterile, Inlet: 0.25 to inch SB, Outlet: 0.25 to inch SB 5/pk Polycap 150 AS 2806 Polycap AS 150, 0.45µm Sterile - Inlet: 5/pk 1/2 Stepped Hosebarb Outlet 1/2 Hosebarb 2807 Polycap AS 150, 0.45µm Sterile - Inlet: 1/2 Stepped Hosebarb Outlet 1/2 Hosebarb 5/pk SPF 75 1/pk SPF 36 1/pk Polycap 36 TF 2601 Polycap TF 36, 0.2µm 5/pk 2601T Polycap TF 36, 0.2µm, Inlet: /pk inch NPT, Outlet: inch NPT 2602S Polycap TF 36, 0.45µm, Inlet: 5/pk Sanitary, Outlet: Sanitary 2603 Polycap TF 36, 1.0µm 5/pk 2603T Polycap TF 36, 1.0µm, Inlet: /pk NPT, Outlet: NPT μm 1/pk μm 1/pk μm 1/pk μm 1/pk μm 1/pk μm 1/pk Polycap 75 TF 2700T Polycap TF 75, 0.1µm, Inlet: /pk inch FNPT, Outlet: inch FNPT 2702M Polycap TF 75, 0.2µm, Inlet: /pk inch MNPT, Outlet: 0.25 inch MNPT 2702T Polycap TF 75, 0.2µm, Inlet: /pk inch FNPT, Outlet: inch FNPT 2703T Polycap TF 75, 0.45µm, Inlet: /pk inch FNPT, Outlet: inch FNPT TF 75, 0.1μm 1/pk TF 75, 0.2μm 1/pk TF 75, 0.45μm 1/pk TF 75, 1μm 1/pk μm 1/pk μm 1/pk Polycap 75 SPF 78

79 Filteration Devices Capsule Filters Polycap 150 TF 2800T Polycap TF 150, 0.1µm, Inlet: /pk inch FNPT, Outlet: inch FNPT 5/pk 2802T Polycap TF 150, 0.2µm, Inlet: /pk inch FNPT, Outlet: inch FNPT 5/pk 2803T Polycap TF 150, 0.45µm, Inlet: /pk inch FNPT, Outlet: inch FNPT 5/pk 2804T Polycap TF 150, 1.0µm, Inlet: /pk inch FNPT, Outlet: inch FNPT 5/pk 2801 Polycap TF 150, 0.2µm Inlet - Sanitary Outlet - Sanitary 5/pk Polycap 36 HD 2611 Polycap HD 36, 0.45/1.0µm 5/pk 2610T Polycap HD 36, 0.2/0.45µm, Inlet: 5/pk inch FNPT, Outlet: inch FNPT 2611T Polycap HD 36, 0.45/1.0µm, Inlet: 5/pk inch FNPT, Outlet: inch FNPT 2612T Polycap HD 36, 1.0/5.0µm, Inlet: 5/pk inch FNPT, Outlet: inch FNPT 2613T Polycap HD 36, 5.0/10.0µm, Inlet: 5/pk inch FNPT, Outlet: inch FNPT 2614T Polycap HD 36, 10.0/20.0µm, Inlet: 5/pk inch FNPT, Outlet: inch FNPT HD 36, 1 μm 1/pk HD 36, 10 μm 1/pk HD 36, 20 μm 1/pk HD 36, 5 μm 1/pk Polycap 75 HD 2710 Polycap HD 75, 0.2/0.45µm Inlet: 5/pk 1/2 Hosebarb Outlet 1/2 Hosebarb 5/pk 2712 Polycap HD 75, 1.0/5.0µm Inlet: 1/2 5/pk Hosebarb Outlet 1/2 Hosebarb 5/ pk 2713 Polycap HD 75, 5.0/10.0µm Inlet: 5/pk 1/2 Hosebarb Outlet 1/2 Hosebarb 5/pk 2714 Polycap HD 75, 10.0/20.0µm Inlet: 5/pk 1/2 Hosebarb Outlet 1/2Hosebarb 5 /pk 2711T Polycap HD 75, 0.45/1.0µm, Inlet: 5/pk inch FNPT, Outlet: inch FNPT 2712M Polycap HD 75, 1.0/5.0µm, Inlet: 5/pk 0.25 inch MNPT, Outlet: 0.25 inch MNPT 2712T Polycap HD 75, 1.0/5.0µm, Inlet: 5/pk inch FNPT, Outlet: inch FNPT 2713T Polycap HD 75, 5.0/10.0µm, Inlet: 5/pk inch FNPT, Outlet: inch FNPT 2714T Polycap HD 75, 10.0/20.0µm, Inlet: 5/pk inch FNPT, Outlet: inch FNPT HD 75, 1 μm 1/pk HD 75, 10 μm 1/pk HD 75, 20 μm 1/pk HD 75, 5 μm 1/pk Polycap 150 HD 2810 Polycap HD 150, 0.2/0.45µm 5/pk 79

80 Filteration Devices Capsule Filters 2813 Polycap HD 150, 5.0/10.0µm Inlet: 1/2 Hosebarb Outlet 1/2 Hosebarb 2810T 2812T 2813T 2814T Polycap 36 TC Polycap HD 150, 0.2/0.45µm, Inlet: inch FNPT, Outlet: inch FNPT Polycap HD 150, 1.0/5.0µm, Inlet: inch FNPT, Outlet: inch FNPT Polycap HD 150, 5.0/10.0µm, Inlet: inch FNPT, Outlet: inch FNPT Polycap HD 150, 10.0/20.0µm, Inlet: inch FNPT, Outlet: 0.125inch FNPT , 0.1μm Sterile 1/pk , 0.2μm Sterile 1/pk , 0.45μm Sterile 1/pk , 0.1μm Sterile with filling bell , 0.2μm Sterile with filling bell , 0.45μm Sterile with filling bell 5/pk 5/pk 5/pk 5/pk 5/pk 1/pk 1/pk 1/pk , 0.2μm 1/pk , 0.8/0.2μm Sterile 1/pk Polycap 75 TC , 0.1μm Sterile 1/pk , 0.2μm Sterile 1/pk , 0.2μm Sterile with filling bell , 0.8/0.2μm Sterile with filling bell 1/pk 1/pk , 0.45μm Sterile 1/pk , 1.0μm Sterile 1/pk , 0.2μm 1/pk Polycap 150 TC , 0.1μm Sterile 1/pk μm Sterile 5/pk , 0.2μm Sterile 1/pk , 0.45μ 1/pk , 0.8/0.2 Sterile 1/pk Polycap 75 GW , 0.45μ 1/pk GW 75, 0.45μm 100/pk Carbon Cap This filter capsule is suitable for adsorption of organics from air and removal of color, organics, and chlorine from water Carbon Cap 150 1/pk Carbon Cap 75 1/pk Venting Filters Whatman Venting Filters are disposable devices designed and manufactured with a high-purity polypropylene housing to maintain sample purity. Products are available with a choice of filtration media to suit a range of venting applications. Applications Venting (filling) Isolation (incubators, autoclaves, lyophilizers, EtO sterilizers, fermenters) Electronics (gases) Sterivent 36 80

81 Filteration Devices Capsule Filters / Venting Filters 2103 Sterivent 36, 0.2µm Inlet - 3/8 to 1/2 inch Stepped Hosebarb Outlet- 3/8 to 1/2 inch Stepped Hosebarb Sterivent 150 1/pk 2107 Sterivent 150, 0.2µm Inlet -3/8-1/2" Stepped Hosebarb Outlet- 3/8-1/2" Stepped Hosebarb 2108 Sterivent 150, 0.2µm Inlet - Sanitary Outlet - Sanitary 1/pk 1/pk Hepa-Cap 150 1/pk 2809 HEPA-CAP 150, Inlet: 1/2 Hosebarb Outlet 1/2 Stepped Hosebarb 5/pk Hepa-Vent mm Disc 10/pk PolyVENT PolyVENT is an integral filter product for sterile venting of vessels and tanks Polyvent 4 50/pk Polyvent /pk Polyvent 16 10/pk Polyvent /pk Polyvent 500 1/pk HEPA-CAP and HEPA-VENT HEPA filter media are used throughout the scientific, research and industrial environments in a variety of air and gas filtration applications where high retention, dirt-holding capacity, and flow rates are required. *99.97% retention of particles ³ 0.3µm HEPA CAP Hepa-Cap 36 1/pk 2609T Hepa-Cap 36, Inlet: inch FNPT, Outlet, inch FNPT 5/pk HEPA CAP Hepa-Cap 75 1/pk 2709T Hepa-Cap 75, Inlet: inch FNPT, Outlet: inch FNPT 5/pk HEPA CAP

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84 Seed Testing Paper: Whatman seed testing papers are made from pure cellulose without any additives and do not contain any substances which could influence the growth of the seeds. The constant water absorption of the papers ensures the continuous provision of the required amount of water. The contrast of the color seed testing papers makes evaluation easier, particularly for seeds with fine white rootlets or under artificial light. Surface Protectors - Benchkote Benchkote is an absorbent, impermeable material designed to protect laboratory surfaces against hazardous spills. The material features a high-quality, smooth, absorbent Whatman paper, which quickly absorbs liquid spills, and a laminated polyethylene layer that prevents flow through to the working surface. ph Indicator Whatman offers a range of ph indicator and test papers made with traditional Whatman quality. These products combine ease-of-use with unsurpassed accuracy and consistency. The convenience of using indicator papers for the rapid determination of ph values has led to many applications in laboratories and industry. 84

85 Specialty Products Separate the organic from the inorganic. Protect lab surfaces. Test the ph levels in swimming pools. A range of products for a variety of tasks to help you touch lives with safety and security. Whatman offers a range of specialty products to meet your specific testing requirements. Made with traditional Whatman quality, these products combine ease of use with unsurpassed accuracy and consistency. 85

86 Speciality Products Seed Testing Paper Whatman seed testing papers are made from pure cellulose without any additives and do not contain any substances which could influence the growth of the seeds. The constant water absorption of the papers ensures the continuous provision of the required amount of water. The contrast of the color seed testing papers makes evaluation easier, particularly for seeds with fine white rootlets or under artificial light. This makes work easier, improves the results, and saves time. All seed testing papers meet the specifications published by the ISTA (International Seed Testing Association) and AOSA (Association of Official Seed Analysts). For the most common test methods (TP = top of paper, PP = pleated paper) several grades of paper are offered Pleated Strips White, 1000/pk Germination test paper, 50 double pleats, 110 x 20mm Pleated Strips Grey, 1000/pk Germination test paper, 50 double pleats, 110 x 20mm Sheets, Seed testing 1000/pk paper 140 x 200mm Sheets, Seed testing 100/pk paper 110 x 170mm Pleated Strips White, 1000/pk Germination test paper, 50 double pleats, 110 x 20mm, includes wrapping strips Pleated Strips Grey, Germination test paper, 50 double pleats, 110 x 20mm, includes wrapping strips 1000/pk Shark Skin Paper Creped, medium to slow filter paper. Resistant to weak acids and bases. Often used as a protective paper for filter press 86 cloths, as well as in processing of cocoa butter and edible oils. Cat. No. Diameter Qty mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk Speciality Filter Paper for Textile Industry mm mm Ignition Strength Papers Certified for use in measuring the ignition strength of cigarettes according to ASTM standard E mm 100/pk Antibiotic Assay (AA) Discs For determining the type of causal agent of infectious diseases and for checking their sensitivity to antibiotics and

87 Speciality Products chemotherapeutic agents in vitro by means of the inhibition zone determination method. The test discs can be coated with chemotherapeutic agents, placed on the innoculated nutrient agar and incubated. The size of the inhibition zone is a measure for the effectiveness of the substances. Cat. No. Diameter Qty mm 1000/pk mm 1000/pk mm 1000/pk Surface Protectors Benchkote: An absorbent, impermeable material designed to protect lab surfaces against hazardous spills. The material features a highquality, smooth, absorbent Whatman paper, which quickly absorbs liquid spills and a laminated polyethylene layer that prevents flow through to the working surface. Cat. No. Diameter Qty x 570mm 1/pk mm x 50 metres 1/pk mm x 50 metres 1/pk x 570mm 50/pk x 570mm 100/pk Weighing Boats Transfer your samples completely loss-free by simply dropping the entire weighing boat containing the sample into the acid solution in the Kjeldahl flask/digestion tube The fastest, safest and most reliable way to transfer Kjeldahl samples. Cat. No. Diameter Qty Kjeldahl Weighing 100/pk Boat Cat. No. Diameter Qty X150 mm 500/pk X100mm 500/pk Cat. No. Diameter Qty X12 inch 500/pk X3 inch 500/pk X4 inch 500/pk X6inch 500/pk Benchkote Plus: A thicker, more absorbent material for more demanding applications. Cat. No. Diameter Qty x 600mm 50/pk mm x 50 metres 1/pk Lens Cleaning Tissue Whatman lens cleaning tissue is chemically pure and free from silicones and other additives. Most importantly, it can be relied on to safely remove surface moisture and grease. The soft texture of the lens cleaning tissue will not damage lenses or optical surfaces. It is very strong and leaves no fibers. Cat. No. Diameter Qty x 150mm 25/pk x 300mm 100/pk x 570mm 500/pk Specialty Filter paper Cat. No. Diameter Qty cv, 70mm cv, 90mm Ruled Circles, 45mm 100/pk Ruled Circles, 70mm 100/pk Ruled Circles, 90mm 100/pk Ruled Circles, 75mm 100/pk 87

88 Speciality Products 1 PS Phase Separators The Whatman 1 PS Phase Separator is an easy to use highgrade filter paper impregnated with a stabilized silicone that renders it hydrophobic, retaining the aqueous phase and passing the solvent phase through. Cat. No. Diameter Qty mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk mm 100/pk ph Indicator & Test Papers Whatman offers a range of ph indicator and test papers to meet your specific needs. The convenience of using indicator papers for the rapid determination of ph values has led to many applications in laboratories and industry. Features and Benefits Instant ph readings Accurate for a wide range of routine ph testing Inexpensive Convenient and portable for field use a Cat. No. ph Range Qty A /pk A /pk A 4-7 1/pk A /pk A /pk A Reel, Litmus Blue, 7mm 1/pk x 5m A Reel, Litmus Red, 1/pk 7mmx5M A Reel, Phenophthalein, 1/pk 7mmx5M to /pk books of 20 strips 10/pk litmus blue books of 20 strips 10/pk litmus red A 7mmx50M-KI 1/pk A 7mmx50M Lead Acetate 1/pk ph Indicator & Test Papers Cat. No. Diameter Qty mm x5mm 1/pk x 100mm 1/pk x 80mm 1/pk x 80mm 1/pk x100mm 1/pk x100mm 1/pk x100mm 1/pk x100mm 1/pk x100mm 1/pk x 100mm 1/pk strips 10/pk ph range /pk x75mm 100/pk ph range /pk ph range /pk ph range /pk 88

89 Speciality Products Multiwell Plates Application specific plates ad Unifilter Microplates Cat. No. Diameter Qty U WPS P81 pk/ u PSC pk/ U CPS LD.45PVDF pk/ BINDING PLATE pk/ u PSC GF/C pk/ u PSC GF/B pk/ u PSC MBPP pk/ u PSC.45 PP pk/ u PSC.45 CA pk/ u PSC GF/F pk/ u GBPP GF/C pk/ mL GBPP.45 PVDF pk/ mL GBPP CL GF/D pk/ ML PP GF/C pk/ ML PP μm pk/25 MBPP ML PP μm MBPP pk/25 Multiwell Accessories Cat. No. Diameter Qty MIL BLANK pk/ SILICONE RD96 - pk/ UL PSC 100/EA pk/ ORING pk/ ASSIST FRAME 1/EA pk/1 Elutrap System Cat. No. Diameter Qty Starter Kit pk/ System Kit 4-Pak & pk/1 Chamber Electro-Phoresis pk/1 Chamber System Starter Kit & pk/1 Chamber BT-1 Membranes pk/ BT-2 Membranes pk/100 Uniplates Collection Plates Cat. No. Diameter Qty WELL 2ML - 25's pk/ WELL 10ML PP pk/ WL 5ML F PP pk/ Filter 96/2ML Fast Flow pk/ UN u PSC MBPP 25/ pk/ UNIPL 24-10ML PP ST W/L RB- pk/25 25's Uniseal 2MIL Blank pk/ Uniseal Silicone RD96 - pk/ UL PSC 100/EA pk/ Uniseal Oring pk/ Vacuum-Assist Frame pk/1 89

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97 Quick product finder Product Page No. Grade 1 Cellulose filter papers - Qualitative... 9 Grade 41 Ashless filter papers - Quantitative Grade 42 Cellulose filter papers - Quantitative Grade 541 Hardened Ashless filter papers - Quantitative Binder Free Glass Microfiber Filters - GF/A Series PM 2.5 Air Monitoring Membranes Mixed cellulose ester membranes EPM Cellulose Extraction Thimbles Grade 3MM Chr - Cellulose Chromatography Papers Grade 1 Chr - Cellulose Chromatography Papers Specimen Collection Paper FTA Cards DMPK Cards GD/X Syringe Filters Puradisc Syringe Filters Uniflo Syringe Filters Benchkote Surface Protectors Lens Cleaning Tissue

98 Page No. INDEX Page No. 1 CHR Roll CHR Sheets PS Phase Separators CHR Sheets /2 Folded filters CHR Sheets ET CHR Chroma Reel MM CHR Blotting Paper Place Manifold, PVC CHR Roll CHR Sheets Cellulose thimbles Dry Rak without Velcro Protein Saver Snap-Apart Card A Acid Treated Low Metal TCLP Circles Anotop 10 Syringe Filter AS220 2-place Vacuum filtration manifold for MBS I Ashless Clippings Autoclaving bags for MBS I plastic funnels Autovial Syringeless Filter 5ml, 0.2μm PTFE Autovial Syringeless Filter 5ml, 0.45μm Nylon Autovial Syringeless Filter 5ml, 0.45μm PTFE Autovial Syringeless Filter 5ml, 0.45μm PVDF B Benchkote Surface Protector Biohazard Labels C Carbon-Cap Cellulose Thimbles Clonesaver Card D Desiccant packs Dispenser for 100 ml and 350 ml funnels for MBS I E EPM2000 Circles F Foil Barrier Ziplock Bags FTA Classic Card FTA Elute Micro Card FTA Kit FTA Micro Card FTA Plant Kit FTA Plantsaver FTA Purification Reagent G GB 003 Blotting Paper GB005 Blotting Paper GD/X 25 Syringe Filter, 0.2μm Nylon GF/A Circles GF/A Sheets GF/B Circles GF/C Circles GF/D Circles GF/F Circles Glass Fiber Thimbles Glassine Envelopes Grade 1 Circles Grade 1 Sheets Grade 113 Circles Grade 113 Sheets Grade 17Chr Circles Grade 2 Circles Grade 2 Sheets Grade 3 Circles Grade 4 Circles Grade 4 Sheets Grade 40 Circles Grade 41 Circles Grade 42 Circles Grade 43 Circles Grade 44 Circles Grade 5 Circles Grade 50 Circles Grade 52 Circles Grade 54 Circles Grade 54 Sheets

99 Page No. INDEX Page No. Grade 540 Circles Grade 541 Circles Grade 541 Sheets Grade 542 Circles Grade 6 Circles Grade 934-AH Circles & RTU H Harris Uni-Core Punch 1.25mm Harris Uni-Core Punch 3.0mm Hepa-Cap I Indicating FTA Classic Indicating FTA Minicard Indicator paper, Litmus blue K Kjeldahl Weighing Boat L Lens Cleaning Tissue M MBS I plastic funnel of PP Membrane Circles, Anodisc Membrane Holder, Glass Support Mini UniPrep Compressor Mini UniPrep Syringeless Filter Mini UniPrep Syringeless Filter G Multi-Barrier Pouch Multigrade GMF Circles N Nuclepore Membrane Circles P ph Indicators, Book ph Indicators, Standard Full Range PM2.5 Membrane, PTFE Polycap AS Polycap HD Polycap TF Polycap TF Polydisc HD PolyVent Q Quartz filters QM-A Sheets QM-H R RC55 Membrane Circles, Regenerated cellulose RC58 Membrane Circles, Regenerated cellulose Replacement Cutting Mat Rezist Syringe Filter S Seed testing paper SG81 Sheets Shark Skin Circles Spartan syringe filter Spot Holder U Uniflo syringe filters V Vacuguard Vaccum filtration apparatus

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101 Terms and Conditions Rates Prices are net ex-godown. Packing Packing, forwarding, freight, postage etc. will be charged extra. Taxes VAT, Sales Tax, Central Sales Tax and Octroi will be charged extra as applicable. Payment Cash against delivery or through bank or as per negotiation. Claims Claims for breakages and shortages in transit will not be entertained. Insurance Consignment can be insured on specific, request by customers at their cost. Any increase in Central or State Government levies such as customs duty, sales tax and octroi etc will be charged to the customers. Complaints Complaints, if any, should be communicated to us within 30 days from the date of Invoice. 101