YMC-Triart C18. YMC Co., Ltd. Versatile Hybrid Silica Based HPLC Column PB-0052E. Versatile hybrid silica material Particle synthesis by Microreaction

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Nigel Nichols
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SEPARATION TECHNOLOGY PB-5E YMC-Triart C8 Versatile

silica material Particle synthesis by Microreaction

organic/inorgan ic hybrid si lic a Stationary : C8 (as

Bon ding : polym yme ric ty pe End -capping : mul ti-

1 SEPARATION TECHNOLOGY PB-5E YMC-Triart C8 Versatile Hybrid Silica Based HPLC Column Versatile hybrid silica material Particle synthesis by Microreaction Multistage endcapping Specification ions Base : organic/inorgan ic hybrid si lic a Stationary : C8 (as USP-L) Par ticle : m, 5 m Por e : nm Carbo bon % : % Bon ding : polym yme ric ty pe End -capping : mul ti- sta ge end -ca pping ph ran ge : ph ~ YMC Co., Ltd.

Versatile Hybrid Silica Based HPLC Column YMC-Triart C8 Rugged, long lasting, hybrid particle technology Integration of triple artistic technologies Feature Benefit Particle synthesis by

2 Versatile Hybrid Silica Based HPLC Column YMC-Triart C8 Rugged, long lasting, hybrid particle technology Integration of triple artistic technologies Feature Benefit Particle synthesis by Microreaction Versatile hybrid silica material Multistage endcapping YMC-Triart C8 Integration of Three Technologies YMC-Triart is a hybrid silica based ODS column emphasizing durability, low operating pressure, and excellent performance. YMC-Triart C8 has been developed and is manufactured using state-of-the-art technologies that define materials, granulation and surface modification. Versatile hybrid material YMC-Triart C8 is a multi-layer hybrid particle consisting of two types of layers: a silica based layer (inorganic) and a hybrid polymer based layer (organic). This layered structure contributes to Triart s combined physical durability and chromatographic performance. Image structure for hybrid-silica gel

New granulation process by microreactor technology Homogeneous and uniform particle Triart C8 Narrow pore distribution 45 Competitor s hybrid particle 4 Hybrid particle for Triart d Vp/d logd 5

in low operating pressure and YMC-Triart C8 has a very narrow pore size distribution that very reproducible surface modification making for the enables narrow and reproducible peak shapes for

3 New granulation process by microreactor technology Homogeneous and uniform particle Triart C8 Narrow pore distribution 45 Competitor s hybrid particle 4 Hybrid particle for Triart d Vp/d logd 5 Silica particle for conventional product 5 Hybrid particle for competitor's product Pore size (nm) Use of microreactor technology produces homogeneous and 5 5 uniform particles that results in low operating pressure and YMC-Triart C8 has a very narrow pore size distribution that very reproducible surface modification making for the enables narrow and reproducible peak shapes for analytes. ultimate in column to column and lot to lot reproducibility. Surface modification technology Multi-stage end-capping End-capping methods employed for production of conventional products typically employ a single end-capping compound in one chemical step to "neutralize" highly reactive silanols. Unfortunately, highly reactive silanols are easily hydrolyzed and contribute to poor durability. Additionally, low reactive silanols end-cap stubbornly, producing a surface modification that is often non-reproducible, and leading to peak tailing. YMC-Triart C8 solves this problem by using a innovative, newly developed "measured multi-stage/multi-compound surface modification" technology which results in the thorough end-capping of all silanols that are accessible to analytes. Reaction image for multi-stage end-capping B fore Before Bef EC STEP CùĀ Evaluation of silanol activity Ingredients in cough/ cold medication Triart C8 6 STEP CùĀ CùĀ. Chlorpheniramine. Dextromethorphan. Propyl p-hydroxybenzoate 4 Silanol group. End-capping (EC) group Q998C min CùĀ 6 4 End-capping by traditional method Peak tailing for basic compounds STEP. Q998E min 5 µm, 5 X. mmi.d. Column mm KHPO4-KHPO4 (ph 6.9)/acetonitrile (65/5) Eluent Flow rate.45 ml/min Temperature 4 Detection UV at 5 nm YMC-T -Triart C8

4 Quality Control Excellent reproducibility YMC-Triart C8 exhibits excellent lot to lot reproducibility for all types of compounds including difficult to chromatograph molecules that exhibit basic and coordination compound behavior. Basic compounds Coordination compounds Lot. E Lot. E Lot. D Lot. D Lot. C Lot. C Lot. B Lot. B. Chlorpheniramine. Dextromethorphan. Hinokitiol. Propyl p-hydroxybenzoate. Methyl benzoate Lot. A min min Lot. A Rigid control of theoretical plate number (N) and tailing factor (Tf) are exhibited for each YMC-Triart C8 packed column. Theoretical plate number 5 5 Theoretical plate number Tailing factor Column number Tailing factor Low back-pressure Free from pressure problem Standard ODS Best balance between and k Column presure (MPa) Column pressure and solvents Organic solvent rate (%) Triart C8, MeOH/W Triart C8, ACN/W Pro C8, MeOH/W Pro C8, ACN/W YMC-Triart C8 is designed for use in all conditions. Even with higher viscosty methanol, YMC-Triart C8 can operate with lower pressure (typically % less than conventional columns). Hydrogen-bonding capacity (caffeine/benzene) Comparison of /k of columns in market Standard ODS The most versatile columns as first-choice. Eluent : MeOH/W (8/) [amylbenzene], MeOH/W (/7) [caffeine, benzene] (S985A) Hydrophobicity k (amylbenzene) Conventional hybrid silica based ODS columns tend to be less hydrophobic than silica based columns. YMC-Triart C8 has a favorable carbon balance and may be used as a "versatile first-choice" method development column. 4

5 Great durability Wide ph range and long lifetime Rate of theoretical plate number Phosphate buffer (ph.5, 4 C) % % 8% 6% 4% % Silica based C8 YMC-Triart C8 Hybrid-silica based C8 % 4 5 Time (hr) Rate of theoretical plate number Triethylamine (ph.5, 4 C) % % 8% 6% 4% % Silica based C8 YMC-Triart C8 % Time (hr) Rate of theoretical plate number ph 6.9, 7 C % % 8% 6% 4% % Silica based C8 YMC-Triart C8 Silica based C8 alkaline-resistant type % Time (hr) Rate of theoretical plate number ph, 7 C % % 8% 6% 4% % YMC-Triart C8 Silica based C8 Hybrid-silica based C8 The columns are kept in acetonitrile/water/tfa (/9/) 7 C, and tested for performance every hours. % Time (hr) YMC-Triart C8 shows great chemical stability provided by newly developed hybrid silica. Even in high ph or high temperature conditions, the lifetime of YMC-Triart C8 is more than X greater than conventional ODS columns. Reduced column bleed Operate at high sensitivity Bleed test by LC/MS.E+9 8.E+8 Scan ES+ TIC Triart C8 No column 4.E+8.E Bleeding of stationary phase can often contribute to high background signals when employing MS and other hyphenated detection techniques. In the example cited above, use of a x 5 mm YMC-Triart C8 column shows no contribution to the signal observed by the MS positive total ion current (TIC) trace. Such performance allows for low limits of detection (high S/N ratio) for MS and other detection techniques. The inherent hybrid particle strength is also superior to conventional silica materials for detection techniques that are sensitive to particle degradation. min YMC-T riart C8 5

6 Excellent resolution Without adsorption and tailing Analysis of basic compounds YMC-Triart C8 Ingredients in a cough/cold medication.. Q998C Silica based C8 Brand D5. Chlorpheniramine Dextromethorphan Q98C Silica based C8 Brand N J9K Hybrid-silica based C8 Brand I8 Q9F Propyl p-hydroxybenzoate YMC-Triart C8's innovative surface modification technology results in excellent peak shapes even for the basic compounds that often exhibit tailing shapes on conventional silica and hybrid silica based ODS columns min Analysis of coordination compounds YMC-Triart C8 Hinokitiol.. Q995G Silica based C8 Brand N Hybrid-silica based C8 Brand I min J9B Q9A Hinokitiol Methyl benzoate YMC-Triart C8 has an extremely low level of metal impurities, much lower than conventional products. YMC-Triart C8 is able to provide excellent peak shape for coordination compounds. Analysis of acidic compounds YMC-Triart C8 Organic acid Q965C Hybrid-silica based C8 Brand G min Q977A YMC-Triart C8 is synthesized utilizing methodology borrowed from microreactor technology. This synthesis technique allows for a reduction of impurities that contribute to peak tailing during the analysis of some types of acidic compounds. 6

7 Excellent versatility buffer-independency Comparison of clemastine analysis with conventional columns mm phosphate buffer (ph 6.7)/organic solvent mm CHCOONH4/organic solvent methanol acetonitrile methanol acetonitrile YMC-Triart C8 Tf.97 Tf. Tf. Tf.6 F988H7 F988I7 F988F7 F987G7 Silica based C8 Brand C Tf. F977H7 Tf.56 F977I7 Tf. F977F7 Tf.4 F98G7 Silica based C8 Brand D5 Tf.9 F977H7 Tf.8 F977I7 Tf.5 F974F7 Tf. F974G7 Silica based C8 Brand I6 Tf. Tf.87 Tf. Tf.88 F97H7 F97I7 F97F7 F97G7 Hybrid-silica based C8 Brand I8 Tf.4 Tf.88 Tf. Tf.78 F97H7 F97I7 F97F7 F97G7... min min min min Clemastine Clemastine is a well known basic compound which can easily tail on conventional ODS columns. YMC-Triart C8 can separate clemastine sharply using many buffer/solvent combinations. Improvement of loadability Minimizing strong solvent/sample loading effects Influence on peak shapes by injection volume YMC-Triart C8 solvent for sample injection volume. µl.5 µl. µl. µl Hybrid-silica based C8 Silica-based C8 Peak anomaly at.5 µl.. Propranolol 5 µg/ml Verapamil 5 µg/ml.5..5 min YMC-Triart C8 can tolerate larger injection volumes of samples containing strong solvents (e.g., acetonitrile) while allowing for better peak shape than traditional columns. This can be important for difficult to solubilize samples that need higher concentrations of organic solvent for solubilization prior to analysis. YMC-T riart C8 7

8 Application Utilization of micron YMC-Triart C8 5 %B,. ml/min, 5.7 MPa 5 5%B,. ml/min, 5.6 MPa 5 insufficient separation Eluent optimization 5 Baseline separation, But long analysis time 5 5 R96J min.. Diquat Paraquat R97C min %B,.4 ml/min,. MPa Downsizing 8% reduction in run time compared to 5 micron,. x 5 mm column with modest (. MPa) operating pressure. R9B 4 5 min Ordering Information Analytical column YMC-Triart C8 Analytical column YMC-Triart C8 Guard cartridge holder Guard cartridge column (inner diameter. mm : -pack inner diameter 4. mm : -pack) Cartridge holder will need to be purchased separately before using this product for the first time Semi-preparative column YMC-Actus Triart C8 YMC Co., Ltd. sales@ymc.co.jp YMC America, Inc. info@ymcamerica.com YMC Europe GmbH info@ymc.de YMC India Private Ltd. sales@ymcindia.com URL URL URL URL K-

Introducition of a new product from Inertsil Series

Introducition of a new product from Inertsil Series is a C8 column developed focusing on superb inertness to basic, acidic, neutral and chelating compounds, which can virtually provide perfect peak shapes