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1 i n t ro d u c i n g t h e n e w research antibodies catalog from w w w. s c b t. c o m Toll Free: Phone: Fax: The Power to Question

2 Costimulation T Cell DC Cytokines Elisa & Elispot Toll-Like Receptors TrueBlot Stem Cell Regulatory Foxp3 Antibodies Extensively Characterized, Validated & Published Mouse/Rat Foxp3 Human Foxp3 C57BL/6 spleen cryosections were stained with anti-mouse/rat Foxp3 antibody, FJK-16s (cat ). FJK-16s-PE - (cat# ) Resting Splenocytes -FITC - (cat# ) BALB/c splenocytes were stained with anti- (clone RM4-5) and antimouse/rat Foxp3 (clone FJK-16s). PCH101-PE - (cat# ) PBMCs 5-APC - (cat# ) Human PBMCs were stained with anti- 5 (clone BC96) and anti-human Foxp3 (clone PCH101) A B C Immunoblotting of PBMC (A), BALB/c splenocytes (B), or recombinant human Foxp3 fusion protein (C); probed with ebio7979 (cat ). NRRF-30 FJK-16s ebio7979 Mouse/Rat Zn finger/lz Forkhead Domain PCH101 Exon 2 alternatively spliced in human FJK-16s 236A/E7 ebio7979 Human/non-Human Primate ebioscience Foxp3 antibodies have been epitope-mapped and immunophenotyped. Intracellular co-staining using several Foxp3 antibodies demonstrates labeling of the same cell population, thereby confirming specificity. Additionally, the western blot data indicate the absence of the identified human splice variant in mouse. Description Clone Application Format Human/NHP Foxp3 PCH Flow, WB, IHC Purified, Biotin, PE-Cy5, FITC, PE, APC, Alexa Fluor Conjugates, Pacific Blue, Treg Staining Kit Human Foxp3 236A/E Flow, WB, IHC Purified, Biotin, PE, APC, Alexa Fluor 488 Human/Mouse Foxp3 ebio7979 Flow, WB, IHC Purified, Alexa Fluor 488 Mouse/Rat Foxp3 FJK-16s 1-12 Flow, WB, IHC Purified, Biotin, PE-Cy5, FITC, PE, APC, Alexa Fluor Conjugates, Pacific Blue, Treg Staining Kit Mouse Foxp3 NRRF-30 Flow PE Mouse GITR DTA-1 NU, Flow, IP Purified, FG Purified (azide-free), Biotin, PE, APC, FITC Mouse GITRL ebioygl386 Flow, IHC Purified, Biotin, PE NHP = non-human primate *Alexa Fluor and Pacific Blue are registered trademarks of and licensed under patents assigned to Molecular Probes, Inc. for research use only. 1) J. Exp. Med. 202: ) J. Immunol. 175: ) J. Immunol. 175: ) J. Immunol. 175: ) J. Exp. Med. 202: ) Immunity. 28: ) J. Exp. Med. 202: ) J. Immunol. 175: ) J. Exp. Med. 202: ) Blood. 106: ) Eur. J. Immunol. 35: ) J. Exp. Med. 202: ) J. Immunol. 175: ) Blood [Dec Epub]. 15) Eur. J. Immunol. 35: ) Leukemia [Epub]. 17) Clin. Cancer Res. 11: WEB ORDER FAX TECH TECH SUPPORT tech@ebioscience.com INQUIRIES contact@ebioscience.com

3 Bio-Plex suspension array system Bio-Plex, Leading Life Science Discovery Advance your understanding of cell biology and mechanisms of disease with the integrated Bio-Plex system Bio-Plex is the most widely cited multiplex assay platform with research applications in Alzheimer s and Parkinson s disease, diabetes, obesity, cancer, asthma, cystic fibrosis, autoimmune disease, viral infections, and vaccine development. The Bio-Plex system enables you to quantitate up to 100 different analytes in one sample with unsurpassed sensitivity, precision, and accuracy, giving you the power to understand complex relationships between proteins in normal and disease states. For more information, visit us on the Web at Newest Solution Human cytokine 27-plex panel includes the following cytokine targets: IL-1, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9,, IL-12 (p70), IL-13, IL-15, IL-17, eotaxin, basic FGF, G-CSF, GM-CSF, IFN-, IP-10, MCP-1 (MCAF), MIP-1, MIP-1, PDGF-BB, RANTES, TNF-, VEGF. Mouse cytokine 23-plex and rat cytokine 9-plex panels are also available. Visit us on the Web at discover.bio-rad.com Call toll free at BIORAD ( ); outside the US, contact your local sales office.

4 RAT IgM HEAVY CHAIN RAT KAPPA/LAMBDA LIGHT CHAIN CD13 CD16 3 FSH BETA 2 FSH BETA 3 FSH-INTACT Cancer bax Bcl-2 CALCITONIN RECEPTOR CD14 CD16 CD19 BETA 2 MICROGLOBULIN CD56 CD61 CD79a GLUCOCORTICOID RECEPTOR hcg CORE FRAGMENT hcg-alpha 2 EPITOPE hcg-alpha 3 EPITOPE c-erb B-3 0 Cytokines hcg-alpha 4 EPITOPE EGF R 2 5 hcg-alpha 5 EPITOPE EGF R (INTRACELLULAR) 3 CD114 hcg-alpha 6 EPITOPE We have GST thousands 3 of reliable, CD116 hcg-beta 4 EPITOPE Her2/neu 4 CLASS II CD119 hcg-beta 7 EPITOPE MCM5 8 CD120a hcg-holo MOESIN 5 CD181 hcg-holo-c3 EPITOPE MPO high quality antibodies that CD182 can INSULIN OESTROGEN RECEPTOR BETA 1 CD56 CD195 PC1/3 OESTROGEN RECEPTOR BETA 2 35a 10 4 CLASS II 5 8 CDw218a T3 be used in your research. 5 EGF R TSH RECEPTOR FGF BASIC CYCLIN D1 Breast Cancer IFN GAMMA IGF-BINDING PROTEIN Infection FIV-gp120 Just CD5 Not Bcl-2 FIV-p24 IL-6 CALCITONIN RECEPTOR IL-12 (p40 SUBUNIT) HEPATITIS B ANTIGEN CD7 EGF R IL-2 HSV TYPE 1 gd GST IL-4 HSV TYPE I & TYPE II COMMON EPITO CD9 Her2/neu IL-6 HSV TYPE I & TYPE II gb MOESIN IP-10 SALMONELLA BROAD REACTIVITY CD13 OESTROGEN RECEPTOR BETA 1 TNF ALPHA SALMONELLA ENTERITIDIS CD14 IL-6 Target Species: MOUSE SALMONELLA TYPHIMURIUM CD16 CD9 CD115 SIMIAN VIRUS 5 CD18 10 CD19 CD18 IFN GAMMA Cytoskeletal 3 CD18 (ACTIVATION EPITOPE) TUBULIN (ALPHA) 4 4 IL-2 All At Once. 9 9 IL-4 Serum Reagents 3 4 TNF ALPHA DONKEY SERUM 4 9f Target Species: RAT GOAT SERUM 8 CD54 CD122 GUINEA PIG SERUM 4 CD61 5 HORSE SERUM 5 CD66e Target Species: MOUSE MOUSE SERUM 9f CD146 CD115 PIG SERUM CD RABBIT (BABY) COMPLEMENT CD54 p53 aa10-16 IFN GAMMA RABBIT (NORMAL) COMPLEMENT CD56 p53 aa20-25 RABBIT HAEMOLYTIC SERA CD61 p53 aa26-35 IL-2 RABBIT SERUM CD66e CD79a CD115 CD138 CD146 CD a p53 aa46-55 p53 aa56-65 p53 aa p53 aa p53 aa p53 aa Hematology FACTOR H IL-4 TNF ALPHA Target Species: RAT CD122 Cardiac markers MYELOPEROXIDASE TROPONIN T RAT SERUM SHEEP SERUM Secondary Reagents APAAP COMPLEX FITC HI STAR DETECTION KIT HISTAR MOUSE ON MOUSE DETECTION KIT STREPTAVIDIN:ALK.PHOS. Leukemia MPO CD5 Kidney function BETA 2 MICROGLOBULIN Hormones ACTH aa11-24 ACTH N-TERMINAL STREPTAVIDIN:APC STREPTAVIDIN:FITC STREPTAVIDIN:HRP STREPTAVIDIN:PE-TR STREPTAVIDIN:RPE STREPTAVIDIN:RPE-CY5 Our Toll-like Receptor Antibodies: AbD Serotec Offers You: GOAT IgG (Fc) HAMSTER HAMSTER IgG LAP IgG (Fc) RABBIT IgG MOUSE IgG (Fc) MOUSE IgA (ALPHA CHAIN) MOUSE IgE HEAVY CHAIN MOUSE IgG MOUSE IgG (H&L) MOUSE IgG (H&L) (MULTISPECIES ADSORBED) MOUSE IgG (RAT ADSORBED) MOUSE IgG HEAVY CHAIN MOUSE IgG,A,M (HUMAN ADSORBED) MOUSE IgG1 MOUSE IgG1 HEAVY CHAIN MOUSE IgG2a MOUSE IgG2a HEAVY CHAIN MOUSE IgG2b MOUSE IgG2b HEAVY CHAIN MOUSE IgG3 MOUSE IgG3 HEAVY CHAIN MOUSE IgM (HUMAN ADSORBED) MOUSE IgM HEAVY CHAIN PAP OVI SHEEP IgG (HUMAN, MOUSE, RABBIT ADSORBED) RAT IgG2a RAT IgA RAT IgG (H&L) (HUMAN, RABBIT, BOVINE ADSORBED) RAT IgG (MOUSE ADSORBED) RAT IgG1 RAT IgG1 HEAVY CHAIN RAT IgG2a RAT IgG2a HEAVY CHAIN RAT IgG2b RAT IgG2b HEAVY CHAIN RAT IgG2c RAT IgG2c HEAVY CHAIN RAT IgM RAT IgM HEAVY CHAIN RAT KAPPA/LAMBDA LIGHT CHAIN Epitope Tags c-myc GST HISTIDINE TAG MBP (MALTOSE BINDING PROTEIN) V5 TAG Immunoglobulins IgA (SECRETORY) RITUXIMAB HUMAN HUMAN IgG (GAMMA CHAIN) HUMAN IgG (H&L) (HAMSTER, MOUSE, IgA IgA SECRETORY CHAIN IgE IgM J CHAIN LAMBDA LIGHT CHAIN MOUSE MOUSE IgG MOUSE IgG (H&L) MOUSE IgG (RAT ADSORBED) MOUSE IgG,A,M (HUMAN ADSOR MOUSE IgM (HUMAN ADSORBED MOUSE KAPPA LIGHT CHAIN NEGATIVE CONTROL PURIFIED MOUSE IgE RAT RAT IgG RAT IgG (MOUSE ADSORBED) RAT KAPPA CHAIN (IgKa PREFER Accessories AEC BUFFER AEC CONCENTRATE ALAMAR BLUE ANTIBODY DILUENT ANTIGEN UNMASKING FLUID AQUEOUS MOUNTING MEDIUM AVIDIN/BIOTIN BLOCKING SYSTE BLOCK ACE DAB BUFFER DAB CONCENTRATE DIALYSIS CASSETTE ELISA BSA BLOCK ELISA COATING BUFFER ELISA DILUENT RABBIT ELISA GENERAL BUFFER ELISA IgM REDUCING BUFFER ELISA NEPTUNE BUFFER ELISA SYNBLOCK ELISA ULTRABLOCK ERYTHROLYSE (RED CELL LYSING BUFFER) FAST RED BUFFER FAST RED TABLETS LEUCOPERM MOUSE ISOTYPING KIT MYCOPLASMA REMOVAL AGENT PEROXIDE BLOCKING REAGENT RAT ISOTYPING KIT STUF MARK 2 (ACID) STUF MARK 2 (BASIC) STUF MARK 2 (UNIVERSAL) TBS/TWEEN GST Her2/neu MOESIN MPO/ OESTROGEN RECEPTOR BETA 1 OESTROGEN RECEPTOR BETA 2 JOI 04/2006 TollLike MPO CD5 CD13 CD14 Hormones ACTH aa11-24 ACTH N-TERMINAL STREPTAVIDIN:FITC STREPTAVIDIN:HRP STREPTAVIDIN:PE-TR STREPTAVIDIN:RPE STREPTAVIDIN:RPE-CY5 HUMAN HUMAN IgG (GAMMA CHAIN) HUMAN IgG (H&L) (HAMSTER, MOUSE IgM J CHAIN OESTROGEN RECEPTOR BETA 2 1 (800) USA & Canada +49 (0) Germany, Austria & Switzerland Scandinavia & Baltic States France +44 (0) UK, Ireland, Benelux & Worldwide

5 IMMUNOLOGY OF JOURNAL THE Sponsoring a Symposium or Conference... You can place a quarter-, half-, or full-page advertisement in The Journal of Immunology for upcoming symposia or conferences. It s a great way to spread the word about forthcoming events! You can include important information, such as registration Steve West, Professional Media Group (PMG), at ext. 14 or steve@promedgroup.net! "! +,-.., ' / ) * * * contact information. For space availability and pricing, please contact fees, timetables, speakers, and all necessary!"#$% &' ( ) *

6 +/5+ +/5- Human PBMCs stained with FITC and FOXP3 (clone 206D) PE C57BL/6 splenocytes stained with FOXP3 (clone 150D) Alexa Fluor 488. C57BL/6 mouse splenocytes stained with 5 PE and GITR (clone YGITR765) FITC Applications: Applications: Applications: Flow Cytometry, IHC, WB Flow Cytometry, IHC, WB Flow Cytometry