T H E J O U R N A L O F C E L L B I O L O G Y
|
|
|
- Prosper Horn
- 5 years ago
- Views:
Transcription
1 Supplemental material Egan et al., T H E J O U R N A L O F C E L L B I O L O G Y Figure S1. Localization of dynein to microtubule plus ends requires kinesin-1 but not kinesin-3s. Micrographs showing the localization of dynein-gfp and histone H1 mcherry-labeled nuclei in wild-type, kinesin-1, kinesin-3/unca, and kinesin-3/uncb hyphae. In strains lacking kinesin-1, dynein comets are absent from the microtubule plus ends and instead decorate the length of the microtubules. Dashed lines indicate the outline of the hyphae. Bar, 5 µm. Figure S2. Endosome and peroxisome transport is unaffected by deletion of kinesin-3/uncb. (A and B) Micrographs showing the localization of GFP- Rab5/RabA labeled endosomes (A) and Pex11/PexK-GFP labeled peroxisomes (B) in wild-type (top images) and kinesin-3/uncb (bottom images) hyphae. Endosomes and peroxisomes are normally distributed throughout the length of the hyphae. GFP -tubulin microtubules are also visible in the kinesin-3/uncb PexK-GFP image. Dashed lines indicate the outline of the hyphae. Bars, 5 µm. S1
2 Figure S3. Kinesin-3/UncA accumulates in the hyphal tip in the absence of dynein. (A C) In wild-type hyphae (A), kinesin-3/unca-gfp particles are uniformly distributed (left images) and move bidirectionally, as indicated by kymographs generated from time-lapse videos (right images). In dynein hyphae (B and C), kinesin-3/unca particles are mislocalized to the hyphal tip (left images) and are largely immotile, as shown in a representative kymograph (B, right), except for rare plus-end directed movements (C, right). Dashed lines indicate the outline of the hyphae. The locations of microtubule plus ends in the hyphal tip are indicated by white plus signs. Figure S4. Tagging dynein or Lis1 with GFP does not affect function. (A) Targeted deletion of the sole genomic copy of dynein or Lis1 severely affects hyphal growth and colony size, leading to a nud phenotype. (B) Strains expressing the dynein heavy chain tagged with three tandem copies of GFP at its C terminus grow similarly to wild type. (C) Strains expressing Lis1 tagged with GFP at its C terminus grow similarly to wild type. Bars, 1 cm. S2
3 Figure S5. Dynactin is required for endosome and dynein motility. (A and B) Micrographs (left images) and representative kymographs (right images) showing the localizations and motilities of GFP-Rab5/RabA endosomes (A) and dynein-3xgfp (B) in wild-type (top images) and p150 (bottom images) hyphae. GFP-Rab5/RabA endosomes in wild-type hyphae (A, top left) are uniformly distributed and move bidirectionally (A, top right), whereas in p150, they accumulate in the hyphal tip (A, bottom left) and are nonmotile (A, bottom right). In wild-type hyphae, dynein-3xgfp (B, top left) localizes to microtubule plus ends and to discrete puncta decorating the microtubule length, which move bidirectionally (B, top right). In p150 hyphae (B, bottom left), dynein- 3xGFP appears diffuse in the cytoplasm and is nonmotile (B, bottom right). Dashed lines indicate the outline of the hyphae. The locations of microtubule plus ends in the hyphal tip are indicated by white plus signs. S3
4 Video 1. GFP-Rab5/RabA labeled endosome dynamics in a wild-type hypha. Long-distance bidirectional transport of GFP- Rab5/RabA endosomes in a wild-type hypha. Images were analyzed by time-lapse wide-field fluorescence microscopy using a custom-built microscope (DeltaVision OMX). Frames were taken every 162 ms for 32 s. Frame rate is 10 frames per second. Video 2. Pex11/PexK-GFP labeled peroxisome dynamics in a wild-type hypha. Sporadic anterograde and retrograde peroxisome movement in a wild-type hypha. Images were analyzed by time-lapse wide-field fluorescence microscopy using a custom-built microscope (DeltaVision OMX). Frames were taken every 162 ms for 15.9 s. Frame rate is 10 frames per second. Video 3. GFP-Rab5/RabA labeled endosome dynamics in a dynein hypha. Nonmotile GFP-Rab5/RabA endosomes accumulate in the hyphal tip of a dynein deletion mutant. Images were analyzed by time-lapse wide-field fluorescence microscopy using a custom-built microscope (DeltaVision OMX). Frames were taken every 142 ms for 28 s. Frame rate is 10 frames per second. Video 4. GFP-Rab5/RabA labeled endosome dynamics in a kinesin-3/unca hypha. GFP-Rab5/RabA endosomes are nonmotile in the absence of kinesin-3/unca. Images were analyzed by time-lapse wide-field fluorescence microscopy using a custom-built microscope (DeltaVision OMX). Frames were taken every 142 ms for 28 s. Frame rate is 10 frames per second. Video 5. Dynein-3xGFP dynamics in a wild-type hypha. Dynein-3xGFP particles moving bidirectionally throughout the length of a wild-type hypha. Images were analyzed by time-lapse wide-field fluorescence microscopy using a custom-built microscope (DeltaVision OMX). Frames were taken every 94 ms for 9.3 s. Frame rate is 10 frames per second. The arrowhead points to the hyphal tip. Video 6. Lis1-GFP and mcherry-rab5/raba labeled endosomes in a wild-type hypha. Lis1-GFP and mcherry-rab5/raba labeled endosome dynamics in a wild-type hypha. Images were analyzed by time-lapse wide-field fluorescence microscopy using a custom-built microscope (DeltaVision OMX). Frames were taken every 130 ms for 4.7 s. Frame rate is 10 frames per second. S4
5 Table S1. A. nidulans strains used in this study Strain Genotype Source LO1704 [hhoa::gfp::afribo]; ribob2; pabaa1; pyroa4; pyrg89;, fawn B. Oakley LO1915 [hhoa::mcherry::afpyrg]; wa::[gfp::tuba::afpyroa]; ribob2; pyroa4; pyrg89; B. Oakley MAD2056 argb2::[argb::alca(p)::gfp::raba]; ya2; pantob100 X. Xiang; Zhang et al., 2010 TFJ 20.6 argb2::[argb::alca(p)::gfp::raba]; ya2; pantob100 M. Peñalva trpc::[pexk::gfp::afpyro]; pyroa M. Hynes; Hynes et al., 2008 LZ12 [GFP::nudA]; pyroa; pyrg89; X. Xiang; Zhuang et al., 2007 RPA197 [ kina::afpyro]; [hhoa::mcherry::afpyrg]; [pexk::gfp::afpyro]; pyroa4; pyrg89; nkua::bar/argb RPA199 [ unca::afpyrg]; [hhoa::mcherry::afpyrg]; [pexk::gfp::afpyro]; pyroa4; pyrg89; nkua::bar/argb RPA218 [hhoa::mcherry::afpyrg]; [pexk::gfp::afpyro]; pyroa; pyrg89; nkua::bar/argb RPA230 [ uncb::bar]; [hhoa::mcherry::afpyrg]; [pexk::gfp::afpyro]; wa::[gfp::tuba::afpyroa]; pyroa4; pyrg89; nkua::bar/argb RPA261 [GFP::nudA]; [ kina::afpyro]; [hhoa::mcherry::afpyrg]; pyroa4; pyrg89; nkua::bar RPA262 [GFP::nudA]; [ uncb::bar]; [hhoa::mcherry::afpyrg]; pyroa4; pyrg89; nkua::bar RPA263 [GFP::nudA]; [ unca::afpyrg]; [hhoa::mcherry::afpyrg]; pyroa4; pyrg89; RPA265 [GFP::nudA]; [hhoa::mcherry::afpyrg]; pyroa4; pyrg89; nkua::bar RPA269 [ uncb::bar]; [hhoa::mcherry::afpyrg]; argb2::[argb::alca(p)::gfp::raba]; maybe nkua RPA282 [hhoa::mcherry::afpyrg]; argb2::[argb::alca(p)::gfp::raba]; pyroa4 RPA313 [EB1/AN2862::mCherry::Afribo]; [hhoa::mcherry::afpyrg]; wa::[gfp::tuba::afpyroa ]; ribob2; pyroa4; pyrg89; RPA349 [nuda::3xgfp::afpyrg]; [hhoa::gfp::afribo]; ribob2; pabaa1; pyroa4; pyrg89; fwa; RPA362 [ kina::afpyro]; [EB1/AN2862::mCherry::Afribo]; [hhoa::mcherry::afpyrg]; wa::[gfp::tuba::afpyroa]; ribob2; pyroa4; pyrg89; nkua::bar/argb RPA363 [ unca::afpyrg]; [EB1/AN2862::mCherry::Afribo]; [hhoa::mcherry::afpyrg]; wa::[gfp::tuba::afpyroa]; ribob2; pyroa4; pyrg89; nkua::bar RPA365 [ uncb::bar]; [EB1/AN2862::mCherry::Afribo]; [hhoa::mcherry::afpyrg]; wa::[gfp::tuba::afpyroa]; ribob2; pyroa4; pyrg89; nkua::bar/argb RPA368 [ nuda::bar]; [hhoa::mcherry::afpyrg]; [pexk::gfp::afpyroa]; pyrg89; pyroa4; RPA395 argb2::[argb::alca(p)::gfp::raba]; [hhoa::mcherry::afpyrg]; ya1; nkua::bar RPA401 [nuda::3xgfp::afpyrg]; argb2::[argb::alca(p)::mcherry::raba]; pyrg89; pyroa4; nkua::bar RPA413 [ nuda::bar]; [EB1/AN2862::mCherry::Afribo]; [hhoa::mcherry::afpyrg]; wa::[gfp::tuba::afpyroa]; ribob2; pyroa4; pyrg89; RPA432 [ kina::afpyro]; [hhoa::mcherry::afpyrg]; argb2::[argb::alca(p)::gfp::raba]; pyroa4, nkua::bar RPA442 [ unca::afpyrg]; [hhoa::mcherry::afpyrg]; argb2::[argb::alca(p)::gfp::raba]; nkua::bar RPA443 [ nuda::bar]; [hhoa::mcherry::afpyrg]; argb2::[alca(p)::gfp::raba]; RPA453 [ nudf::afpyrg]; argb2::[argb::alca(p)::gfp::raba]; pyrg89; RPA464 [TagGFP2::rabA::AfpyrG]; ya1; pyrg89; pyroa4; pabaa1; RPA480 [TagGFP2::rabA::AfpyrG]; [ nudf::afpyro]; ya1; pyrg89; pyroa4; pabaa1; RPA503 [ nudf::afpyro]; [nuda::3xgfp::afpyrg]; wa::[gfp::tuba::afpyroa ]; pyrg89; pyroa4; nkua::bar RPA508 argb2::[argb::alca(p)::mcherry::raba]; [nudf::gfp::afpyrg]; ya1; pyrg89; pyroa4; pabaa1; nkua::bar RPA524 [ nudf::afpyrg]; [nuda::3xgfp::afpyrg]; argb2::[argb::alca(p)::mcherry::raba]; pyrg89; pyroa4; nkua::bar RPA527 [nuda::3xgfp::afpyrg]; ya::[gpda(p)::mcherry::flag::pts1::afpyro]; pyrg89; pyroa4; RPA592 [ nudf::bar]; ya::[gpda(p)::mcherry::flag::pts1::afpyro]; [nuda::3xgfp:: AfpyrG]; pyrg89; pyroa4: RPA597 [unca::taggfp2::afribo]; ribob2; pyrg89; pyroa4; RPA660 [ nudm::afpyro]; [TagGFP2::rabA::AfpyrG]; ya1; pabaa1; pyrg89; pyroa4; RPA661 [ nudm::afpyro]; argb2::[argb::alca(p)::mcherry::raba]; [nuda::3xgfp::afpyrg]; pyrg89; pyroa4; nkua::bar RPA662 [ nuda::afpyrg]; [unca::taggfp2::afribo]; ribob2; pyrg89; pyroa4; RPA663 [p25/an5022::taggfp2::afpyrg]; argb2::[argb::alca(p)::mcherry::raba]; pyrg89; pyroa4; nkua::bar All promoters are the endogenous promoter, unless otherwise stated. S5
2 SI J. R. Larson et al.
Figure S1 Methods used to identify conditional drug-sensitive suppressors of nima1. Diagram of the genetic screen performed and the general steps involved to identify suppressors of nima1 temperature sensitivity.
T H E J O U R N A L O F C E L L B I O L O G Y
T H E J O U R N A L O F C E L L B I O L O G Y Supplemental material Craft et al., http://www.jcb.org/cgi/content/full/jcb.201409036/dc1 Figure S1. GFP -tubulin interacts with endogenous -tubulin. Ciliary
Figure legends for supplement
Figure legends for supplement Supplemental Figure 1 Characterization of purified and recombinant proteins Relevant fractions related the final stage of the purification protocol(bingham et al., 1998; Toba
T H E J O U R N A L O F C E L L B I O L O G Y
T H E J O U R N A L O F C E L L B I O L O G Y Supplemental material Allison et al., http://www.jcb.org/cgi/content/full/jcb.201211045/dc1 Figure S1. Spastin depletion causes increased endosomal tubulation.
Supplemental Information. Glutamylation Regulates Transport, Specializes. Function, and Sculpts the Structure of Cilia
Current Biology, Volume 27 Supplemental Information Glutamylation Regulates Transport, Specializes Function, and Sculpts the Structure of Cilia Robert O'Hagan, Malan Silva, Ken C.Q. Nguyen, Winnie Zhang,
Supplemental Data. Liu et al. (2013). Plant Cell /tpc
Supplemental Figure 1. The GFP Tag Does Not Disturb the Physiological Functions of WDL3. (A) RT-PCR analysis of WDL3 expression in wild-type, WDL3-GFP, and WDL3 (without the GFP tag) transgenic seedlings.
SUPPLEMENTARY INFORMATION
a 14 12 Densitometry (AU) 1 8 6 4 2 t b 16 NMHC-IIA GAPDH NMHC-IIB Densitometry (AU) 14 12 1 8 6 4 2 1 nm 1 nm 1 nm 1 nm sirna 1 nm 1 nm Figure S1 S4 Quantification of protein levels. (a) The microtubule
T H E J O U R N A L O F C E L L B I O L O G Y
Supplemental material Wang et al., http://www.jcb.org/cgi/content/full/jcb.201405026/dc1 T H E J O U R N A L O F C E L L B I O L O G Y Figure S1. Generation and characterization of unc-40 alleles. (A and
Supplementary Materials for
www.sciencemag.org/cgi/content/full/science.1245533/dc1 Supplementary Materials for A Mechanism for Reorientation of Cortical Microtubule Arrays Driven by Microtubule Severing Jelmer J. Lindeboom, Masayoshi
T H E J O U R N A L O F C E L L B I O L O G Y
T H E J O U R N A L O F C E L L B I O L O G Y Supplemental material Rainero et al., http://www.jcb.org/cgi/content/full/jcb.201109112/dc1 Figure S1. The expression of DGK- is reduced upon transfection
SUPPLEMENTARY INFORMATION
DOI: 1.18/ncb46 a Damage Fracture s s 7 5 16 1 9 26 5 µm 5 µm b Position of photo-damage site in cells 15 Counts 1 5-14 -12-1 -8-6 -4-2 Distance from damage site to tip (µm) Supplementary Figure 1 Effect
T H E J O U R N A L O F C E L L B I O L O G Y
T H E J O U R N A L O F C E L L B I O L O G Y Supplemental material Rodríguez-Fraticelli et al., http://www.jcb.org/cgi/content/full/jcb.201203075/dc1 Figure S1. Cell spreading and lumen formation in confined
Gene disruption and construction of C-terminally tagged strains
Supplementary information Supplementary Methods Gene disruption and construction of C-terminally tagged strains A PCR-based gene targeting method (Bähler et al., 1998; Sato et al., 2005) was used for constructing
Wt (Col-0) pom2-1 Wt (Ler) pom2-3 pom2-5. pom2-4 (0%) pom2-1 (0%)
Figure 1 Wt (Col-0) Wt (Ler) pom2-3 pom2-5 pom2-2 pom2-4 B Wt (0%) (0%) Wt (4.5%) (4.5%) Wt (0%) (0%) Wt (4.5%) (4.5%) (4.5%) Figure 1. Phenotypes of pom2 and wild type (Wt) seedlings after germination
Fig. S1. Endocytosis of extracellular cargo does not depend on dia. (A) To visualize endocytosis, fluorescently labelled wheat germ agglutinin
Fig. S1. Endocytosis of extracellular cargo does not depend on dia. (A) To visualize endocytosis, fluorescently labelled wheat germ agglutinin (WGA-Alexa555) was injected into the extracellular perivitteline
Supplementary Figures 1-6
1 Supplementary Figures 1-6 2 3 4 5 6 7 Supplementary Fig. 1: GFP-KlpA forms a homodimer. a, Hydrodynamic analysis of the purified full-length GFP-KlpA protein. Fractions from size exclusion chromatography
MT minus end catastrophe
DOI: 1.138/ncb3241 MT minus end catastrophe frequency [s -1 ].2.15.1.5 control mgfp- TPX2 * * mgfp- TPX2 mini Supplementary Figure 1. TPX2 reduces catastrophes at the microtubule minus ends. Modified box-and-whiskers
T H E J O U R N A L O F C E L L B I O L O G Y
T H E J O U R N A L O F C E L L B I O L O G Y Supplemental material Monteiro et al., http://www.jcb.org/cgi/content/full/jcb.201306162/dc1 Figure S1. 3D deconvolution microscopy analysis of WASH and exocyst
SUPPLEMENTARY INFORMATION
DOI: 10.1038/ncb3374 Supplementary Figure 1 staccato mutations affect a late step of the tube fusion process. (a) Immunostaining of stage 15 embryos labeled with anti- Dys (green in merge) and a probe
Neuronal Activity and CaMKII Regulate Kinesin-Mediated Transport of Synaptic AMPARs
Neuron Supplemental Information Neuronal Activity and CaMKII Regulate Kinesin-Mediated Transport of Synaptic AMPARs Frédéric J. Hoerndli, Rui Wang, Jerry E. Mellem, Angy Kallarackal, Penelope J. Brockie,
over time using live cell microscopy. The time post infection is indicated in the lower left corner.
Title of file for HTML: Supplementary Information Description: Supplementary Figures and Supplementary Table Title of file for HTML: Supplementary Movie 1 Description: Fusion of NBs. BSR cells were infected
unc-33/crmp and ankyrin organize microtubules and localize kinesin to polarize
Supplementary Information unc-33/crmp and ankyrin organize microtubules and localize kinesin to polarize axon-dendrite sorting Tapan A. Maniar 1, Miriam Kaplan 1, George J. Wang 2, Kang Shen 2, Li Wei
SUPPLEMENTARY INFORMATION
Clustering of a kinesin-14 motor enables processive retrograde microtubule-based transport in plants NATURE PLANTS www.nature.com/natureplants 1 2 NATURE PLANTS www.nature.com/natureplants NATURE PLANTS
T H E J O U R N A L O F C E L L B I O L O G Y
T H E J O U R N A L O F C E L L B I O L O G Y Supplemental material Karotki et al., http://www.jcb.org/cgi/content/full/jcb.201104040/dc1 Figure S1. Eisosome proteins form highly stable filaments of variable
Nature Biotechnology: doi: /nbt Supplementary Figure 1
Supplementary Figure 1 High dynamic range (HDR) imaging of live cells with spinning disk confocal microscopy. During image acquisition, z-sections, encompassing most of the cellular volume, are captured
JCB. Supplemental material THE JOURNAL OF CELL BIOLOGY. Kimura et al.,
Supplemental material JCB Kimura et al., http://www.jcb.org/cgi/content/full/jcb.201503023/dc1 THE JOURNAL OF CELL BIOLOGY Figure S1. TRIMs regulate IFN-γ induced autophagy. (A and B) HC image analysis
Microtubules. Forms a sheet of protofilaments that folds to a tube Both tubulins bind GTP
Microtubules Polymerize from a-tubulin/ß-tubulin dimers Hollow tube of 13 protofilaments In vitro- filament number varies In vivo- always 13 protofilaments Forms a sheet of protofilaments that folds to
Nature Biotechnology: doi: /nbt.4166
Supplementary Figure 1 Validation of correct targeting at targeted locus. (a) by immunofluorescence staining of 2C-HR-CRISPR microinjected embryos cultured to the blastocyst stage. Embryos were stained
T H E J O U R N A L O F C E L L B I O L O G Y
T H E J O U R N A L O F C E L L B I O L O G Y Supplemental material Yamaguchi et al., http://www.jcb.org/cgi/content/full/jcb.201009126/dc1 S1 Figure S2. The expression levels of GFP-Akt1-PH and localization
Mutants of Aspergillus nidulans deficient in nuclear migration during hyphal growth and conidiation
MiCrObiOlOgy (1 994), 140, 1 169-1 174 Printed in Great Britain Mutants of Aspergillus nidulans deficient in nuclear migration during hyphal growth and conidiation A. John Clutterbuck Tel: +44 41 339 8855
JCB. Supplemental material THE JOURNAL OF CELL BIOLOGY. Paul et al.,
Supplemental material JCB Paul et al., http://www.jcb.org/cgi/content/full/jcb.201502040/dc1 THE JOURNAL OF CELL BIOLOGY Figure S1. Mutant p53-expressing cells display limited retrograde actin flow at
Supplemental Information. Pacer Mediates the Function of Class III PI3K. and HOPS Complexes in Autophagosome. Maturation by Engaging Stx17
Molecular Cell, Volume 65 Supplemental Information Pacer Mediates the Function of Class III PI3K and HOPS Complexes in Autophagosome Maturation by Engaging Stx17 Xiawei Cheng, Xiuling Ma, Xianming Ding,
Supplementary Information. c d e
Supplementary Information a b c d e f Supplementary Figure 1. atabcg30, atabcg31, and atabcg40 mutant seeds germinate faster than the wild type on ½ MS medium supplemented with ABA (a and d-f) Germination
Liu et al., http :// /cgi /content /full /jcb /DC1
Supplemental material JCB Liu et al., http ://www.jcb.org /cgi /content /full /jcb.201506081 /DC1 THE JOU RNAL OF CELL BIO LOGY Figure S1. Identification of sorf-1 and sorf-2. (A) RNAi of ZK563.5 increases
Supporting Information
Supporting Information Engineering of an unnatural natural product by swapping polyketide synthase domains in Aspergillus nidulans Ting Liu, Yi-Ming Chiang, Amber D. Somoza, Berl R. Oakley, and Clay C.
Comparative functional proteomics of MAP Kinase signalling. in Magnaporthe oryzae
Comparative functional proteomics of MAP Kinase signalling in Magnaporthe oryzae Submitted by Romain Huguet to the University of Exeter as a thesis for the degree of Doctor of Philosophy, in Biological
T H E J O U R N A L O F C E L L B I O L O G Y
Supplemental material Prashar et al., http://www.jcb.org/cgi/content/full/jcb.201304095/dc1 T H E J O U R N A L O F C E L L B I O L O G Y Figure S1. FBT phagocytosis in cells expressing PM-GFP. (A) T-PC
Supplementary Figure 1. Confirmation of sirna in PC3 and H1299 cells PC3 (a) and H1299 (b) cells were transfected with sirna oligonucleotides
Supplementary Figure 1. Confirmation of sirna in PC3 and H1299 cells PC3 (a) and H1299 (b) cells were transfected with sirna oligonucleotides targeting RCP (SMARTPool (RCP) or two individual oligos (RCP#1
Supplemental Material
Supplemental Material 1 Figure S1. Phylogenetic analysis of Cep72 and Lrrc36, comparative localization of Cep72 and Lrrc36 and Cep72 antibody characterization (A) Phylogenetic alignment of Cep72 and Lrrc36
Lecture 13. Motor Proteins I
Lecture 13 Motor Proteins I Introduction: The study of motor proteins has become a major focus in cell and molecular biology. Motor proteins are very interesting because they do what no man-made engines
A versatile and efficient gene targeting system for Aspergillus nidulans. L. Murray, Michael J. Hynes, Stephen A. Osmani* and Berl R.
Genetics: Published Articles Ahead of Print, published on December 30, 2005 as 10.1534/genetics.105.052563 A versatile and efficient gene targeting system for Aspergillus nidulans Tania Nayak*, Edyta Szewczyk*,
Supporting Information. Table of Contents
Electronic Supplementary Material (ESI) for Chemical Science. This journal is The Royal Society of Chemistry 2015 Supporting Information Spatial Regulation of a Common Precursor from Two Distinct Genes
Fig. S1. Phosphorylation of eif2 by PEK-1 is increased in ire-1 mutants. Fig. S2. ire-1
Fig. S1. Phosphorylation of eif2a by PEK-1 is increased in ire-1 mutants. Representative western blot of phosphorylated eif2a and tubulin of day-0 wild-type animals and ire-1 mutants treated with control,
Supplementary Materials for
www.sciencesignaling.org/cgi/content/full//59/ra7/dc1 Supplementary Materials for Dok-7 ctivates the Muscle Receptor Kinase MuSK and Shapes Synapse Formation kane Inoue, Kiyoko Setoguchi, Yosuke Matsubara,
GM130 Is Required for Compartmental Organization of Dendritic Golgi Outposts
Current Biology, Volume 24 Supplemental Information GM130 Is Required for Compartmental Organization of Dendritic Golgi Outposts Wei Zhou, Jin Chang, Xin Wang, Masha G. Savelieff, Yinyin Zhao, Shanshan
The definitive version is available at www3.intersci. Supporting_Information.pdf (Supporting Information) Instructions for use
Title Nonmuscle myosin II folds into a 10S form via two po Kiboku, Takayuki; Katoh, Tsuyoshi; Nakamura, Akio; K Author(s) Masayuki CitationGenes to Cells, 18(2): 90-109 Issue Date 2013-02 Doc URL http://hdl.handle.net/2115/53054
Supplementary Figure S1. Immunodetection of full-length XA21 and the XA21 C-terminal cleavage product.
Supplementary Information Supplementary Figure S1. Immunodetection of full-length XA21 and the XA21 C-terminal cleavage product. Total protein extracted from Kitaake wild type and rice plants carrying
Septins AspA and AspC Are Important for Normal Development and Limit the Emergence of New Growth Foci in the Multicellular Fungus Aspergillus nidulans
EUKARYOTIC CELL, Jan. 2010, p. 155 163 Vol. 9, No. 1 1535-9778/10/$12.00 doi:10.1128/ec.00269-09 Copyright 2010, American Society for Microbiology. All Rights Reserved. Septins AspA and AspC Are Important
SUPPLEMENTARY INFORMATION
Nanomechanical mapping of first binding steps of a virus to animal cells David Alsteens, Richard Newton, Rajib Schubert, David Martinez-Martin, Martin Delguste, Botond Roska and Daniel J. Müller This PDF
The Plasmodium berghei translocon of exported proteins reveals spatiotemporal dynamics of tubular extensions
Supplementary Information for: The Plasmodium berghei translocon of exported proteins reveals spatiotemporal dynamics of tubular extensions Joachim M. Matz, Christian Goosmann, Volker Brinkmann, Josephine
Supplementary material
Supplementary material Manuscript Title Multiple effects of a commercial Roundup formulation on the soil filamentous fungus Aspergillus nidulans at low doses: evidence of an unexpected impact on energetic
Nature Structural & Molecular Biology: doi: /nsmb Supplementary Figure 1
Supplementary Figure 1 Impaired ITS2 processing results in mislocalization of foot-factors to the cytoplasm. (a) Schematic representation of ITS2 processing. The endonuclease Las1 initiates ITS2 processing
FtsEX is required for CwlO peptidoglycan hydrolase activity during cell wall elongation in Bacillus subtilis
FtsEX is required for CwlO peptidoglycan hydrolase activity during cell wall elongation in Bacillus subtilis Jeffrey Meisner, Paula Montero Llopis, Lok-To Sham, Ethan Garner, Thomas G. Bernhardt, David
Supplemental Fig. 1: PEA-15 knockdown efficiency assessed by immunohistochemistry and qpcr
Supplemental figure legends Supplemental Fig. 1: PEA-15 knockdown efficiency assessed by immunohistochemistry and qpcr A, LβT2 cells were transfected with either scrambled or PEA-15 sirna. Cells were then
Supplementary Data Supplementary Figures
Supplementary Data Supplementary Figures Supplementary Figure 1. Pi04314 is expressed during infection, each GFP-Pi04314 fusion is stable and myr GFP-Pi04314 is removed from the nucleus while NLS GFP-Pi04314
Supplementary Figure 1. Espn-1 knockout characterization. (a) The predicted recombinant Espn-1 -/- allele was detected by PCR of the left (5 )
Supplementary Figure 1. Espn-1 knockout characterization. (a) The predicted recombinant Espn-1 -/- allele was detected by PCR of the left (5 ) homologous recombination arm (left) and of the right (3 )
T H E J O U R N A L O F C E L L B I O L O G Y
Supplemental material Kunduri et al., http://www.jcb.org/cgi/content/full/jcb.201405020/dc1 T H E J O U R N A L O F C E L L B I O L O G Y Figure S1. Subcellular localization and interactions of PAPLA1.
Figure S1, related to Figure 1. Characterization of biosensor behavior in vivo.
Developmental Cell, Volume 23 Supplemental Information Separase Biosensor Reveals that Cohesin Cleavage Timing Depends on Phosphatase PP2A Cdc55 Regulation Gilad Yaakov, Kurt Thorn, and David O. Morgan
Supplemental Data. Borg et al. Plant Cell (2014) /tpc
Supplementary Figure 1 - Alignment of selected angiosperm DAZ1 and DAZ2 homologs Multiple sequence alignment of selected DAZ1 and DAZ2 homologs. A consensus sequence built using default parameters is shown
Expanded View Figures
The EMO Journal mediated aggrephagy reconstitution Gabriele Zaffagnini et al Expanded View Figures Figure EV1. and ubiquitinpositive proteins spontaneously cluster in solution. Representative micrographs
Mechanisms for focusing mitotic spindle poles by minus end directed motor proteins
JCB: ARTICLE Mechanisms for focusing mitotic spindle poles by minus end directed motor proteins Gohta Goshima, 1 François Nédélec, 2 and Ronald D. Vale 1 1 The Howard Hughes Medical Institute and the Department
Supplemental Data. Wang et al. (2017). Plant Cell /tpc NIP1;2 NIP7;1
Supplemental Data. Wang et al. (217). Plant Cell 1.115/tpc.16.825 NIP1;1 NIP1;2 NIP2;1 NIP3;1 NIP4;1 NIP5;1 NIP6;1 NIP7;1 Supplemental Figure 1. Distinct Localization of NIPs in Root Epidermal Cells. (Supports
Molecular properties of the N-terminal extension of the fission yeast kinesin-5, Cut7
Molecular properties of the N-terminal extension of the fission yeast kinesin-5, Cut7 M. Edamatsu Department of Life Sciences, Graduate School of Arts and Sciences, the University of Tokyo, Meguro-ku,
monoclonal antibody. (a) The specificity of the anti-rhbdd1 monoclonal antibody was examined in
Supplementary information Supplementary figures Supplementary Figure 1 Determination of the s pecificity of in-house anti-rhbdd1 mouse monoclonal antibody. (a) The specificity of the anti-rhbdd1 monoclonal
JCB. Supplemental material THE JOURNAL OF CELL BIOLOGY. Prospéri et al.,
Supplemental material JCB Prospéri et al., http://www.jcb.org/cgi/content/full/jcb.201501018/dc1 THE JOURNAL OF CELL BIOLOGY Figure S1. Myo1b Tail interacts with YFP-EphB2 coated beads and genistein inhibits
Microtubule Interactions with the Cell Cortex Causing Nuclear Movements in Saccharomyces cerevisiae
Microtubule Interactions with the Cell Cortex Causing Nuclear Movements in Saccharomyces cerevisiae Neil R. Adames and John A. Cooper Department of Cell Biology and Physiology, Washington University School
supplementary information
DOI: 10.1038/ncb1992 Figure S1 (a-b) Verification of actin flow patter using photobleaching of actin:gfp and Lifeact:RFP in migrating dendritic cells (DCs). (a) Actin:GFP and Lifeact:RFP double-trafected
T H E J O U R N A L O F C E L L B I O L O G Y
T H E J O U R N A L O F C E L L B I O L O G Y Supplemental material Feng et al., http://www.jcb.org/cgi/content/full/jcb.201408079/dc1 Figure S1. A modest elevation of disulfide-bonded K14 in primary mouse
Practice Problems 5. Location of LSA-GFP fluorescence
Life Sciences 1a Practice Problems 5 1. Soluble proteins that are normally secreted from the cell into the extracellular environment must go through a series of steps referred to as the secretory pathway.
Flow Cytometry of Microencapsulated Colonies for Genetics Analysis of Filamentous Fungi
INVESTIGATION Flow Cytometry of Microencapsulated Colonies for Genetics Analysis of Filamentous Fungi Lidia Delgado-Ramos,*,,1 Ana T. Marcos,*,1 María S. Ramos-Guelfo,* Leyre Sánchez-Barrionuevo,* Francis
A subclass of HSP70s regulate development and abiotic stress responses in Arabidopsis thaliana
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 Journal of Plant Research A subclass of HSP70s regulate development and abiotic stress responses in Arabidopsis thaliana Linna Leng 1 Qianqian Liang
Supplementary Figure S1
Supplementary Figure S1 Supplementary Figure S1 Subcellular localization of Rab6a and LIS1 in the DRG neurons. (a) Exogenous expression of EGFP-Rab6a(wild-type) in the DRG neurons by the Neon transfection
Alanine-scanning Mutagenesis of Aspergillus -Tubulin Yields Diverse and Novel Phenotypes V
Molecular Biology of the Cell Vol. 12, 2119 2136, July 2001 Alanine-scanning Mutagenesis of Aspergillus -Tubulin Yields Diverse and Novel Phenotypes V M. Katherine Jung,* Natalie Prigozhina,* C. Elizabeth
Supplementary Materials for
www.sciencesignaling.org/cgi/content/full/10/496/eaam6291/dc1 Supplementary Materials for Regulation of autophagy, NF-κB signaling, and cell viability by mir-124 in KRAS mutant mesenchymal-like NSCLC cells
Stargazin regulates AMPA receptor trafficking through adaptor protein. complexes during long term depression
Supplementary Information Stargazin regulates AMPA receptor trafficking through adaptor protein complexes during long term depression Shinji Matsuda, Wataru Kakegawa, Timotheus Budisantoso, Toshihiro Nomura,
mod-1::mcherry unc-47::gfp RME ser-4::gfp vm2 vm2 vm2 vm2 VNC
A mod-1::mcherry unc-47::gfp RME B ser-4::gfp vm2 vm2 VNC vm2 vm2 VN Figure S1 Further details of mod- 1 and ser- 4 reporter expression patterns. (A) Adult head region showing mod- 1::mCherry and unc-
Supplemental Figure 1 Human REEP family of proteins can be divided into two distinct subfamilies. Residues (single letter amino acid code) identical
Supplemental Figure Human REEP family of proteins can be divided into two distinct subfamilies. Residues (single letter amino acid code) identical in all six REEPs are highlighted in green. Additional
The microtubule lattice and plus-end association of Drosophila Mini spindles is spatially regulated to fine-tune microtubule dynamics
MBoC ARTICLE The microtubule lattice and plus-end association of Drosophila Mini spindles is spatially regulated to fine-tune microtubule dynamics Joshua D. Currie a, Shannon Stewman b, Gregory Schimizzi
Supplemental material JCB. Pitaval et al., Cell shape and ciliogenesis Pitaval et al.
Supplemental material THE JOURNAL OF CELL BIOLOGY Pitaval et al., http://www.jcb.org/cgi/content/full/jcb.201004003/dc1 S1 Figure S1. Cell cycle exit analysis. (A) Experimental procedure. RPE1 cells were
Figure S1. USP-46 is expressed in several tissues including the nervous system
Supplemental Figure legends Figure S1. USP-46 is expressed in several tissues including the nervous system Transgenic animals expressing a transcriptional reporter (P::GFP) were imaged using epifluorescence
indicated numbers of pups at day of life (DOL) 10, or embryonic day (ED) B. Male mice of
SUPPLEMENTRY FIGURE LEGENDS Figure S1. USP44 loss leads to chromosome missegregation.. Genotypes obtained from the indicated numbers of pups at day of life (DOL) 10, or embryonic day (ED) 13.5.. Male mice
SAS6-like protein in Plasmodium indicates that conoid-associated apical complex proteins persist in invasive stages within the mosquito vector
SAS6-like protein in Plasmodium indicates that conoid-associated apical complex proteins persist in invasive stages within the mosquito vector Richard J. Wall 1,#, Magali Roques 1,+, Nicholas J. Katris
Characterization Of Motility Alterations Caused By The Impairment Of Dynein/dynactin Motor Protein Complex
University of Central Florida Electronic Theses and Dissertations Masters Thesis (Open Access) Characterization Of Motility Alterations Caused By The Impairment Of Dynein/dynactin Motor Protein Complex
MCBII. Points this page
1. What makes intermediate filaments (IFs) an inefficient track for motor proteins (4 pts)? A. The outer surface of IFs is hydrophobic. B. IFs are nonpolar structures. C. IFs contain coiled coil domains.
Figure S1 early log mid log late log stat.
Figure S1 early log mid log late log stat. C D E F Fig. S1. The chromosome-expressed EI localizes to the cell poles independent of the growth phase and growth media. MG1655 (ptsi-mcherry) cells, which
SUPPLEMENTARY INFORMATION
doi: 10.1038/nature06721 SUPPLEMENTARY INFORMATION. Supplemental Figure Legends Supplemental Figure 1 The distribution of hatx-1[82q] in Cos7 cells. Cos7 cells are co-transfected with hatx-1[82q]-gfp (green)
Deletion of the Dynein Heavy-Chain Gene DYN1 Leads to Aberrant Nuclear Positioning and Defective Hyphal Development in Candida albicans
EUKARYOTIC CELL, Dec. 2004, p. 1574 1588 Vol. 3, No. 6 1535-9778/04/$08.00 0 DOI: 10.1128/EC.3.6.1574 1588.2004 Copyright 2004, American Society for Microbiology. All Rights Reserved. Deletion of the Dynein
SUPPLEMENTARY INFORMATION
doi:10.1038/nature11355 Supplemental Table 1. Gene targeting efficiency in mutants of nonessential genes. This table is presented as a separate Excel file. Supplemental Table 2. Genes with altered expression
Introduction of Biotechnology No.19: Microbial Genetics Fundamentals
Handai Cyber University Introduction of Biotechnology No.19: Microbial Genetics Fundamentals Graduate School of Engineering, Osaka University Graduate School of Information Science, Osaka University International
Supplemental Data includes Supplemental Experimental Procedures, 4 Figures and 5 Movie Legends.
SUPPLEMENTAL DATA Supplemental Data includes Supplemental Experimental Procedures, 4 Figures and 5 Movie Legends. Supplemental Experimental Procedures Dominant negative nesprin KASH (DN KASH) plasmid construction-
Received 10 December 2015 Accepted 29 February 2016 Accepted manuscript posted online 4 March Presented By Swathy J.R
Received 10 December 2015 Accepted 29 February 2016 Accepted manuscript posted online 4 March 2016 Presented By Swathy J.R Vyūha - 'to arrange troops in a battle array' a multi-tier defensive formation
Ac(n cytoskeleton 12/7/11. The Cytoskeleton. Func0ons of the Cytoskeleton. B Visegrady. Elements of the Cytoskeleton
The Cytoskeleton Ac(n cytoskeleton B Visegrady The cytoskeleton is an intricate network of protein filaments that extends throughout the cytoplasm. A skin cell (fibroblast) stained with Coomassie blue.
(A) Schematic depicting morphology of cholinergic DA and DB motor neurons in an L1
SUPPLEMENTAL MATERIALS Supplemental Figure 1 Figure S1. Expression of the non-alpha nachr subunit ACR-2. (A) Schematic depicting morphology of cholinergic DA and DB motor neurons in an L1 animal. Wide-field
Supplemental Data. Ondzighi-Assoume et al. Plant Cell (2016) /tpc G 60
Supplemental ata. Ondzighi-Assoume et al. Plant ell (26).5/tpc.5.946 ABA/Alexa 488 Merge Bright-field luorescence intensity A G 6 Average of fluorescence intensity of endodermis B Z 4 a a RAM 2 c Supplemental
Supporting information
Supporting information Construction of strains and plasmids To create ptc67, a PCR product obtained with primers cc2570-162f (gcatgggcaagcttgaggacggcgtcatgt) and cc2570+512f (gaggccgtggtaccatagaggcgggcg),
Supplemental Information. Kinesin-3 KLP-6 Regulates Intraflagellar. Transport in Male-Specific Cilia. of Caenorhabditis elegans
Current Biology, Volume 21 Supplemental Information Kinesin-3 KLP-6 Regulates Intraflagellar Transport in Male-Specific Cilia of Caenorhabditis elegans Natalia S. Morsci and Maureen M. Barr Figure S1,
fga phenotypes were also analyzed in the tracheal system, the organ responsible for
SUPPLEMENTARY TEXT Tracheal defects fga phenotypes were also analyzed in the tracheal system, the organ responsible for oxygen delivery in the fly. In fga homozygous mutant alleles, we observed defects
Supplemental Fig. 1. Mcr alleles show defects in tracheal tube size and luminal protein accumulation. (A-F) Confocal projections of living stage 15
Supplemental Fig. 1. Mcr alleles show defects in tracheal tube size and luminal protein accumulation. (A-F) Confocal projections of living stage 15 embryos expressing GFP and Verm-RFP in tracheal cells
Autophagy induction [h]
![Autophagy induction [h]](/thumbs/80/80564099.jpg "Autophagy induction [h]")
A SD-N [h]: SD-N [h]: 1 2 4 1 2 4 1 2 4 -GFP 1 2 4 percentage GFP-Atg8 [%] 1 9 8 7 6 5 4 3 2 1 1 2 4 Autophagy induction [h] -13xmyc SD-N [h]: 1 2 4 -GFP -13xmyc 1 prape1 mape1 - - GFP - 13xmyc mape1 [%]