Establishment of hybrid Poplar on a Reclaimed Mine site in West Virginia
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1 Establishment of hybrid Poplar on a Reclaimed Mine site in West Virginia A. Hass, 1 R.S. Zalesny Jr. 1, D. Patel 2, J. Vandevender 3 1 West Virginia State University; Agricultural & Environmental Research Station; Institute, WV 2 U.S. Forest Service; Northern Research Station; Rhinelander, WI 3 U.S. USDA NRCS Plant Material Center, Alderson, WV
2 Land Productivity Picture by Dale K. Ritchey
3 Land Productivity
4 Land Productivity Yield Gap (Actual [management])
5 Land Productivity Yield Ceiling (Potential [genetic]) Yield Gap (Actual [management])
6 Objectives Use phyto-recurrent selection to identify Populus genotypes that grow better on reclaimed mine sites. 6
7 Objectives Use phyto-recurrent selection to identify Populus genotypes that grow better on reclaimed mine sites. Test whether biochar can improve tree survival & growth on reclaim mine site. 7
8 Site Prep and Soil Treatment 8
9 Site Prep and Soil Treatment 9
10 Genomic Group Clone P. deltoides D , C910809, D105, D109, D110, D112, D117, D118, D121, D125, D133, D134 P. trichocarpa T , 0.4.3, 0.6.3, 0.7.5, 1.1.5, 1.2.5, 1.3.1, 2.1.2, 3.2.2, 4.1.3, P. deltoides P. deltoides DD P. deltoides P. nigra DN DN154, DN164, DN17, DN170, DN177, DN182, DN34, DN5, DN70, NE264 P. deltoides P. suaveolens subsp. maximowiczii DM P. nigra P. suaveolens subsp. maximowiczii NM (P. trichocarpa P. deltoides) P. deltoides TDD , , , , , DM105, DM108, DM111, DM112, DM113, DM114, DM115, DM117, NC14103, NC14104, NC14105, NC14106, NC14107 NM2, NM6 NC13536, NC13555, NC13624, NC13728, NC13740, NC13820 Aigeiros Duby P. deltoides Bartr. ex Marsh, P. nigra L. Tacamahaca Spach P. suaveolens Fischer subsp. maximowiczii A. Henry, P. trichocarpa Torr. & Gray
11 Genomic Group Clone P. deltoides D , C910809, D105, D109, D110, D112, D117, D118, D121, D125, D133, D134 P. trichocarpa T , 0.4.3, 0.6.3, 0.7.5, 1.1.5, 1.2.5, 1.3.1, 2.1.2, 3.2.2, 4.1.3, P. deltoides P. deltoides DD P. deltoides P. nigra DN DN154, DN164, DN17, DN170, DN177, DN182, DN34, DN5, DN70, NE264 P. deltoides P. suaveolens subsp. maximowiczii DM P. nigra P. suaveolens subsp. maximowiczii NM (P. trichocarpa P. deltoides) P. deltoides TDD , , , , , DM105, DM108, DM111, DM112, DM113, DM114, DM115, DM117, NC14103, NC14104, NC14105, NC14106, NC14107 NM2, NM6 NC13536, NC13555, NC13624, NC13728, NC13740, NC13820 Aigeiros Duby P. deltoides Bartr. ex Marsh, P. nigra L. Tacamahaca Spach P. suaveolens Fischer subsp. maximowiczii A. Henry, P. trichocarpa Torr. & Gray
12 CYCLE 1 Field (2014) Survival Ht, Diam Biomass Health Cycle 1 Cycle 2 Rep 8 8 Soil treatment 2 0 Clone CYCLE 2 Field (2015) Survival Ht, Diam Health DEPLOYMENT
13 CYCLE 1 Field (2014) Survival Ht, Diam Biomass Health Cycle 1 Cycle 2 Rep 8 8 Soil treatment 2 0 Clone IV = 0.3*MASS *HEIGHT *DIAMETER + 0.2*SURVIVAL + 0.2*HEALTH CYCLE 2 Field (2015) Survival Ht, Diam Health DEPLOYMENT
14 Cycle 1 Probability Values Source of variation Height Diameter Biomass Health Soil Amendment Clone < < < < Soil Treatment Clone Soil Amendment Genomic Group < < < < Soil Treatment Genomic Group
15 Cycle 1; Year 1
16 Cycle 1; Year x 6.0 x 5.4 x 4.5 x
17 Cycle 1; Year 2 Height (cm) b b b b b b a a a a a a Diameter (mm) b b b b b b a a a a a a NC DM Clone DM115 NM6 DM NC NC14105 DM113 DM115 NM DM112 DN170 Clone
18 IV = 0.3*MASS *HEIGHT *DIAMETER + 0.2*SURVIVAL + 0.2*HEALTH
19 IV = 0.3*MASS *HEIGHT *DIAMETER + 0.2*SURVIVAL + 0.2*HEALTH
20 Cycle Genomic 1 Probability Group Values Clone P. deltoides D , C910809, D105, D109, D110, D112, D117, D118, D121, D125, D133, D134 P. trichocarpa T , 0.4.3, 0.6.3, 0.7.5, 1.1.5, 1.2.5, 1.3.1, 2.1.2, 3.2.2, 4.1.3, P. deltoides P. deltoides DD P. deltoides P. nigra DN DN154, DN164, DN17, DN170, DN177, DN182, DN34, DN5, DN70, NE264 P. deltoides P. suaveolens subsp. maximowiczii DM P. nigra P. suaveolens subsp. maximowiczii NM (P. trichocarpa P. deltoides) P. deltoides TDD , , , , , DM105, DM108, DM111, DM112, DM113, DM114, DM115, DM117, NC14103, NC14104, NC14105, NC14106, NC14107 NM2, NM6 NC13536, NC13555, NC13624, NC13728, NC13740, NC13820
21 Cycle 2 Probability Values Source of variation Height Diameter Health Clone Genomic Group
22 Cycle 2 Height (cm) A) a B) a 3.0 b a b 2.5 b c Diameter (mm) a b b a c D DM DN NM TDD 0.0 D DM DN NM TDD Genomic Group Genomic Group
23 Cycle 2 Height (cm) A) a B) a 3.0 b a b 2.5 b c Diameter (mm) a b b a c D DM DN NM TDD 0.0 D DM DN NM TDD Genomic Group Genomic Group
24 Conclusions SOIL AMENDMENT PRACTICES Biochar treatment DID NOT significantly increase tree survival & growth relative to standard soil ripping techniques without amendments PLANT SELECTION Phyto-recurrent selection is a viable tool for selecting superior genotypes P. nigra P. suaveolens subsp. maximowiczii NM performances were superior to all other genomic groups 24
25 Funding Renewable Energy Applications on Surface-Mined Lands Program Administered by: West Virginia Brownfields Assistance Center at Marshall University A Program of: Marshall University s Center for Environmental, Geotechnical & Applied Sciences (CEGAS) Funded by & Under Direction of: West Virginia Division of Energy Office of Coalfield Community Development & Appalachian Regional Commission 25
26 Soil Treatment - Biochar Biochar ph EC Ash Content P S C N O (ms/cm) % weight C/N Ratio Alfalfa Miscanthus Poplar Sorghum Yellow Pine
27 Soil Treatment - Biochar CO 2 Release From Biochar Amended Soil Feedstock Effect 350 mg CO 2 -C kg Soil -1 d Biochar Feedstock
28 Soil Treatment - Biochar CO 2 Release From Biochar Amended Soil Char TN Content mg CO 2 -C kg Soil -1 d R² = Biochar Nitrogen Content (%) Biochar Feedstock
29 Soil Treatment - Biochar Biochar Amended Soil Foxtail Millet Growth Response
30 Soil Treatment - Biochar Biochar Amended Soil Foxtail Millet Growth Response Plant biomass (g pot -1 ) st 1 st cut harvest 2nd 2 st harvest cut 3rd 3 st cut harvest First Total crop biomass Total Biomass y = x R² = Nutrients Treatments Nutrients x2 Treatments 5% Biochar G - Granite fine mix Treatments 2% Biochar G Treatments 5% Biochar G Treatments Total N addition to soil by fertilizer or biochar amendments (mg kg -1 )
31 USDA National Resources Inventory
32
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