COST FA1104 Training School Molecular diagnostics of bacterial diseases
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1 COST FA1104 Training School Molecular diagnostics of bacterial diseases Zurich University of Applied Sciences School of Life Sciences and Facility Management Institute of Natural Resource Sciences Environmental Genomics and Systems Biology Research Group Wädenswil, September 2015 Environmental Genomics and Systems Biology, IUNR, ZHAW Welcome «Grüezi mintenand» Environmental Genomics and Systems Biology, IUNR, ZHAW 2 1
2 Zurich University of Applied Sciences ZHAW ZHAW is one of the leading universities of applied sciences in Switzerland BSc & MSc students in a multidisciplinary teaching and research program in: - Management & Law, - Engineering, - Life Sciences, - Health Professions, - Linguistics - Social work - Architecture, - Psychology ZHAW at a glance: 3 locations (Winterthur, Zurich, Wädenswil) 8 schools 14 master s degree programs 26 bachelor s degree programs 11,186 students 2,984 employees 441 million CHF (cost volume) (Figures from the 2014 Annual Report) Environmental Genomics and Systems Biology, IUNR, ZHAW 3 School of Life Sciences and Facility Management (Wädenswil) Institutes in Wädenswil: Biotechnology Chemistry & Biological Chemistry Facility Management Food & Beverage Innovation Applied Simulation (Informatics) Natural Resource Sciences Topics in research: nutrition/food, health, society and environment Education: 5 Bachelor s degree programs (BSc) 2 consecutive Master s degree programs (MSc) Continuing education: 3 Master of Advanced Studies (MAS) 2 Diploma of Advanced Studies (DAS) Over 30 Certificates of Advanced Studies (CAS) Students (2014): 1,311 BA, 178 MA, 1,290 continuing education Employees (2014): 621, of which 160 are professors and lecturers Environmental Genomics and Systems Biology, IUNR, ZHAW 4 2
3 Three main locations Environmental Genomics and Systems Biology, IUNR, ZHAW 5 Three main locations Campus Reidbach (RC-RV) Environmental Genomics and Systems Biology, IUNR, ZHAW 6 3
4 Three main locations Campus Reidbach (RA) Environmental Genomics and Systems Biology, IUNR, ZHAW 7 Three main locations Campus Grüental (GA-GX) Environmental Genomics and Systems Biology, IUNR, ZHAW 8 4
5 Three main locations Campus Grüental (GA-GX) Environmental Genomics and Systems Biology, IUNR, ZHAW 9 Other location Halbinsel Au (Viticulture Research Group) Environmental Genomics and Systems Biology, IUNR, ZHAW 10 5
6 Institute of Natural Resource Sciences (IUNR) About 500 BSc, 50 MSc, 600 continuing education 190 teaching and research staff 300 research and service provision projects CHF 27 millions revenues (60 % from teaching and 40% from research) Horticulture Phytomedicine EGSB Viticulture Soil Ecology Sustainable energy Ecotechnologie Geoinformatics Ecohydrologie Vegetation analysis Roof greening Geography of food Environmental Genomics and Systems Biology, IUNR, ZHAW 11 Environmental Genomics and Systems Biology Genomics / Transcriptomics / Proteomics / Metagenomics 5 core research and teaching staff 1 postdoc 2 PhD students 2 scientific visitors (PhDs) 2 laboratories: RT 244, molecular biology RT 250, microbiology (BSL2) Environmental Genomics and Systems Biology, IUNR, ZHAW 12 6
7 Laboratory regulations 10:30-11:00 Lab tour and introduction Environmental Genomics and Systems Biology, IUNR, ZHAW 13 Practical information Lockers for personal belongings (5 CHF) on the 2 nd floor Sign attendance list every day Laboratories accessible only with a badge (building open from 7:00 to 19:00) Two coffee breaks per day at Mensa Vista Lunch paid with vouchers at the Mensa Vista (Mensa Grüental on Friday) Laptop required on Thursday afternoon (sequence analysis) Environmental Genomics and Systems Biology, IUNR, ZHAW 14 7
8 COST 1104 Sustainable production of high-quality cherries for the European market 16 April April 2016 Objectives: Development of strategies to safeguard European cherry production Adaptation of cherry cultivation to climate change Promotion of sustainable agriculture Promotion of high-quality fruits for European consumers Work packages: WP1. Genetic resources and Breeding, Genetics and Genomics WP2. Crop Production (Orchard management) WP3. Crop Protection ( Omics approaches) WP4. Socio-economics Environmental Genomics and Systems Biology, IUNR, ZHAW 15 COST 1104 Participating countries International Partner Countries: Australia, New Zealand, Argentina, South Africa Near-Neighboring Countries: Albania, Azerbaijan, Belarus, Morocco, Russia, Armenia, Georgia, Tunisia, Ukraine Environmental Genomics and Systems Biology, IUNR, ZHAW 16 8
9 COST 1104 Activities Meetings (annual/management, special task force) Final meeting 04-08/04/2016 in Greece, Naoussa Short Term Scientific Visits (1 week to 3 months) Only 5 until April 2016 Training Schools WG2 Sensory evaluation 13-16/05/2014 Portugal, Evora WG3 Managing cherry pests in space and time 01-05/09/2014 Greece, Volos WG2 Qualitative physicochemical and biochemical indicators of cherry fruit quality 07-10/06/2015 Cyprus, Larnaca WG3 Molecular diagnostics of bacterial diseases 21-25/09/2015 Switzerland, Wädenswil WG2 Training School Training systems and pruning France 07-11/03/2016 Environmental Genomics and Systems Biology, IUNR, ZHAW 17 Molecular diagnostics of bacterial diseases Task 17 of the MoU: to validate and optimize current visual or molecular detection assays for the most important cherry pathogens. Several types of pests (fruit fly, black cherry aphids) or diseases (both bacterial, fungal or viral) will be considered. Bacterial canker: Pseudomonas syringae species complex dead buds blossom blast leaf spots fruit necrosis canker + gummosis Objective Become familiar with the last and most up to date technologies for phytopathologists, especially with the LAMP technology Environmental Genomics and Systems Biology, IUNR, ZHAW 18 9
10 Molecular diagnostics of bacterial diseases Monday: introduction and classical bacteriology Trainers: Jaap Janse, Monika Kałuzna / Michela Ruinelli, Dmitrijs Konavko, Fabio Rezzonico Tuesday: LAMP Trainers: Steve Milington / Michela Ruinelli Wednesday: MALDI-TOF MS Trainers: Dominik Ziegler, Joël Pothier / Dominik Ziegler, Joël Pothier, Michela Ruinelli Thursday: PCR and sequence analysis Trainers: Theo Smits / Michela Ruinelli, Marilena Palmisano, Fabio Rezzonico Friday: case study Trainers: the trainees! Environmental Genomics and Systems Biology, IUNR, ZHAW 19 Molecular diagnostics of bacterial diseases Lab tour and introduction 10:30-11:00 2 nd floor RT 244, molecular biology RT 250, microbiology (BSL2) Environmental Genomics and Systems Biology, IUNR, ZHAW 20 10
11 Environmental Genomics and Systems Biology, IUNR, ZHAW 21 11
Environmental Genomics and Systems Biology Institute of Natural Resource Sciences Zurich University of Applied Sciences, Wädenswil, Switzerland
Environmental Genomics and Systems Biology Institute of Natural Resource Sciences Zurich University of Applied Sciences, Wädenswil, Switzerland www.zhaw.ch MALDI-TOF MS for microorganism identification:
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