Preparative SFC QDa Applications in Natural Products Research Judith M. Rollinger Ulrike Grienke
|
|
- Nicholas Tate
- 6 years ago
- Views:
Transcription
1 Preparative SFC QDa Applications in Natural Products Research Judith M. Rollinger Ulrike Grienke ÖSTERREICHISCHES TECHNOLOGIE SYMPOSIUM 2016, October 25, 2016
2 Pharmacognosy Group, UNIVIE Plants, fungi, microorganisms, marine organisms, are still the major source of new chemical entities for drug discovery! 64% = derived/inspired by nature 34% = natural cpds /derivatives Sources of drug substances (small chemical entities; , n = 1078): Newman & Cragg (2012) J. Nat. Prod., 75,
3 Research Groups Dirsch Lab: Molecular Targets Research focus: to understand the molecular interaction of natural products with proteins/signaling molecules within cells and to provide cellular models for the identification of bioactive natural products. Rollinger Lab: Phytochemistry & Biodiscovery We use interdisciplinary approaches (e.g. in silico guided, ethnopharmacology-driven) to identify, analyze, and purify multi-component mixtures from plant, fungal, or marine sources aiming at the characterization, the targeted isolation and discovery of bioactive secondary metabolites. Zotchev Lab: Pharmaceutical Biotechnology Discovery of natural product from microorganisms, supported by cutting edge biotechnologyrelated approaches, including genome-based bioprospecting, metabolic engineering and synthetic biology.
4 Aims Pharmaceutical lead and target identification from natural resources Quality control of herbal medicinal products Sustainable production of natural products by optimized extract procedures, chromatographic workflows and biotechnological approaches Educational work and young researchers training
5 ... state-of-the-art equipment! Analytic lab for metabolic profiling, dereplication UPLC systems with different detectors (UV/Vis, DAD, QD, ELSD, CAD) UPLC - triple quadrupole MS with ESI and APCI; UPLC - ion trap mass spectrometer with ESI and APCI ion source GC-MS with EI- and CI-ionization; GC with flame ionization detectors (FID) Different HPLC systems with UV/Vis, DAD and/or ELSD for routine analysis High-performance TLC system with application unit, TLC visualizer and evaluation unit Extraction, fractionation, isolation Prep. supercritical fluid chromatograph (SFC) with parallel detection units (PDA, ELSD, QDa), fraction collector Prep. HPLC systems with autosampler, DAD and fraction collector; High performance counter-current chromatograph (HPCCC) Ultra-performance flash chromatograph combined with PDA and ELSD Chem. characterization of natural compounds/metabolites QTOF-MS with ESI and APCI ion source (with UPLC system; shared with Dep. Pharm. Chem.) NMR spectrometers (400 MHz & 500 MHz) with autosampler and cryoprobe (shared with Dep. Pharm. Chem) UV/VIS spectrophotometers Attenuated total reflection (ATR) - Fourier transform infrared (FTIR) spectrophotometer Biological characterization of natural compounds/multicomponent mixtures Caenorhabditis elegans Screening Plattform (in vivo Model) Different wild type strains (e.g., GFP marked) and genetically modified strains adapteto 96 well plates (for chemical genetics screens & therapeutic screen (neurodegen. diseases) In silico Screening Plattform: Expertise in computer-aided NP drug discovery; VS adapted NP databases (3Dmolecule-DB: > entries); access to modeling software (LigandScout, ROCs, ) See:
6 ... our latest additions To complement our systems H-Class UPLC (with PDA, ELSD, QDa) including fraction manager SFC Prep-15 (with PDA, ELSD, QDa)
7 Instrumental set-up: Waters SFC Prep-15
8 Instrumental set-up: Waters SFC Prep-15
9 Instrumental set-up: Waters SFC Prep-15 CO 2 cylinders chiller N 2 generator
10 Pump for co-solvent and CO 2 pump with different channels for 6 co-solvents pump for CO 2 (cooled with ethylene glycol)
11 Oven, fraction collector, and autosampler column oven: automated column switching and temperature control of up to 10 columns (analytical & preparative) fraction collector & autosampler: accommodates both sample injection & fraction collection
12 Waters H-Class UPLC Our add-on detection configuration QDa MS-based triggering better collection precision by targeting only the mass of interest
13 further chemistries: Silica 2-EP Silica Columns for the SFC Prep-15
14 Columns available in our lab Dimension analytical preparative 4.6 x 250 mm 10 x 250 mm 4.6 x 250 mm 10 x 250 mm 4.6 x 250 mm 10 x 250 mm Silica 2-EP 4.6 x 250 mm 10 x 250 mm particle shape: spherical, particle size: 5 µm
15 Application example: POLYPORES strain 2 strain 1 one species strain 3 different strains different metabolite profile different bioactivity! first evaluation of metabolite profile many triterpenes difficult to detect with HPLC! Profiling with SFC! TLC System: DCM : MeOH : H 2 0 (10 : 1 : 0.25) finding the best source material for bioactive compounds VIS Vanillin/H 2 SO 4 Dresch, P. Aguanno MN, Rosam K, Grienke U, Rollinger JM, Peintner U. (2015): Fungal strain matters: Colony growth and bioactivity of the European medicinal polypores Fomes fomentarius, Fomitopsis pinicola and Piptoporus betulinus. AMB Express 5:4. Pictures:
16 Comparison of metabolite profiles Strain 1 Strain 2 column Viridis BEH, 5 µm 4.6 x 250 mm flow rate make-up flow rate injection volume 5 µl 5 ml/min 3 ml/min Strain 3 sample conc. detection system back pressure 5 mg/ml ELSD 120 bar Strain 4 temp. 40 C Strain 5 Strain 6 Time (min) EtOH (%) best bioactive starting material to isolate the target compound
17 Application example: POLYPORES EtOH crude extract Step 1: liquid/liquid partition Step 2: Silica gel column DCM fraction active > 200 constituents 98% lanostane triterpenes highly similar molecular weight bad UV detection S 1 F 12 active
18 Purification of selected fractions of a polypore fungus HPLC-CAD S 1 F 12 Highly complex triterpene mixture! separated with SFC separated with Flash CC (RP18) SFC LC-parameter: stationary phase: Zorbax (4.6 x 150 mm, 3.5 µm); temperature: 35 C; mobile phase: A= water; B= CH 3 CN; flow rate: 1.0 ml/min; detection: CAD; injection volume: 10 µl; gradient: 0 min: 45% B; 42 min: 80% B; 43 min; 95% B.
19 SFC method development for S 1 F 12 analytical column screening: Viridis SFC columns (particle size: 5 µm, dimension: 4.6 x 250 mm) BEH CSH Fluoro-Phenyl Silica 2-EP co-solvent screening: MeOH EtOH 2-propanol CH 3 CN Time (min) co-solvent (%) flow rate make-up injection volume sample conc. detection system back pressure 5 ml/min 3 ml/min 20 µl 3 mg/ml PDA-ELSD 120 bar temp. 40 C
20 SFC method development for S 1 F 12 S 1 F 12 best separated with EtOH as co-solvent Silica 2-EP CSH FP BEH best separation with BEH column further optimisation
21 Scale-up Scale-up: analytical to preparative mode column Viridis BEH, 5 µm 4.6 x 250 mm (analyt.) 10 x 250 mm (prep.) flow rate make-up flow rate injection volume sample conc. detection 5 ml/min (analyt.) 15 ml/min (prep.) 3 ml/min (analyt.) 5 ml/min (prep.) 20 µl (analyt.) 150 µl (prep.) 3 mg/ml PDA-ELSD Time (min) EtOH (%) system back pressure 120 bar temp. 40 C good separation of single peaks
22 Development of fractionation method S 1 F 12 SFC separation FractionLynx Method: split/collector delay: 9 sec PDA/ELSD delay: 2 sec fractionation based on timed events Collection of 10 fractions
23 Fraction monitoring with RP-HPLC-CAD HPLC monitoring 9 fractions 5, 7, 9 monitoring with RP-HPLC-CAD: LC-parameter: stationary phase: Zorbax (4.6 x 150 mm, 3.5 µm); temperature: 35 C; mobile phase: A= water; B= CH 3 CN; flow rate: 1.0 ml/min; detection: CAD; injection volume: 10 µl; gradient: 0 min: 45% B; 42 min: 80% B; 43 min; 95% B. good separation on Prep-15 system enriched fractions still contain a few peaks
24 Pre-fractionation of S 1 F 12 with Flash chromatography Fraction 6 Fraction 7 HPLC monitoring Fraction 8 column solvents flow rate amount separated detection PuriFlash Column 15 C18 HP 6.0 g gradient: H 2 O/CH 3 CN 5 ml/min ~ 500 mg PDA-ELSD LC-parameter: stationary phase: Zorbax (4.6 x 150 mm, 3.5 µm); temperature: 35 C; mobile phase: A= water; B= CH 3 CN; flow rate: 1.0 ml/min; detection: CAD; injection volume: 10 µl; gradient: 0 min: 45% B; 42 min: 80% B; 43 min; 95% B. Pre-purified fractions further separation via SFC
25 Separation of two co-eluting cpds Fraction 6 A A B B HPLC monitoring SFC-parameter: stationary phase: Viridis BEH, 5 µm 10 x 250 mm; temperature: 40 C; system back pressure: 120 bar, mobile phase: A= CO 2 ; B= EtOH; flow rate: 15.0 ml/min; detection: ELSD; injection volume: 250 µl; gradient: 0 min: 10% B; 8 min: 10% B; 9.5 min: 50% B; 11.5 min: 50% B; 12 min: 10% B; 13 min: 10% B.
26 Separation of two co-eluting cpds Fraction 7 C C HPLC monitoring SFC-parameter: stationary phase: Viridis BEH, 5 µm 10 x 250 mm; temperature: 40 C; system back pressure: 120 bar, mobile phase: A= CO 2 ; B= EtOH; flow rate: 15.0 ml/min; detection: ELSD; injection volume: 250 µl; gradient: 0 min: 8% B; 9.5 min: 8% B; 10 min: 50% B; 11 min: 50% B; 11.5 min: 8% B; 12 min: 8% B.
27 Separation of further cpds Fraction 8 D D HPLC monitoring SFC-parameter: stationary phase: Viridis BEH, 5 µm 10 x 250 mm; temperature: 40 C; system back pressure: 120 bar, mobile phase: A= CO 2 ; B= EtOH; flow rate: 15.0 ml/min; detection: ELSD; injection volume: 250 µl; gradient: 0 min: 8% B; 9.5 min: 8% B; 10 min: 50% B; 11 min: 50% B; 11.5 min: 8% B; 12 min: 8% B.
28 Integration of SFC in NP workflows + SFC is orthogonal to C18 + inexpensive and non-toxic carbon dioxide + easy handling via automated sample collection through FractionLynx Application Manager + beneficial alternative to purification and analytical applications
29 Acknowledgement Dr. Ulrike Grienke Natalie Prenner Julia Zwirchmayr Department of Pharmacognosy, University of Vienna, AUSTRIA Thank you for your attention!
PLC Purification Systems
PLC Purification Systems Preparative HPLC, flash, and CPC in one compact purification system LARGE CAPACITY HIGH VERSATILITY COMPACT FOOTPRINT One system for many columns All the features you need in one
More informationMass Detection for the Chromatographer Waters ACQUITY QDa Detector
Mass Detection for the Chromatographer Waters ACQUITY QDa Detector Warren B Potts III Senior Director Americas Marketing warren_potts@waters.com 2017 Waters Corporation 1 Agenda Overview of technology
More informationImproving Resolution and Column Loading Systematically in Preparative Liquid Chromatography for Isolating a Minor Component from Peppermint Extract
Improving Resolution and Column Loading Systematically in Preparative Liquid Chromatography for Isolating a Minor Component from Peppermint Extract Jo-Ann M. Jablonski and Rui Chen Waters Corporation,
More informationEncompassing Method Development, Small Scale Purification, and Post-Purification Analysis with the Investigator SFC System
Encompassing Method Development, Small Scale Purification, and Post-Purification Analysis with the Investigator SFC System Jacquelyn Runco and Rui Chen, Ph.D. Waters Corporation, Milford, MA, USA A P P
More informationWhat differentiates Dynamic Extractions?
What differentiates Dynamic Extractions? Making liquid stationary phases available for high purity chromatography purification at all scales The difference is the amount of active stationary phase mobile
More informationAgilent SD-1 Purification System. Purify your way SD-1
Agilent SD-1 Purification System Purify your way SD-1 AGILENT SD-1 PURIFICATION SYSTEM PURIFY YOUR WAY WITH HIGH QUALITY SEPARATIONS AT ANY SCALE The Agilent SD-1 Purification System achieves better gradient
More informationAutomated alternating column regeneration on the Agilent 1290 Infinity LC
Automated alternating column regeneration on the Agilent 1290 Infinity LC Increasing throughput using two columns alternatively via an ultra-high pressure 2-position/10-port valve Application Note Environmental
More informationSean M. McCarthy and Martin Gilar Waters Corporation, Milford, MA, U.S. INTRODUCTION EXPERIMENTAL RESULTS AND DISCUSSION
UPLC Separation of DNA Duplexes Sean M. McCarthy and Martin Gilar Waters Corporation, Milford, MA, U.S. INTRODUCTION Over the past 2 years there has been a considerable amount of effort focused on the
More informationHPLC/FLASH Purification ADVANCE THE PACE OF YOUR DISCOVERY TO ACHIEVE THE RESULTS OF TOMORROW
HPLC/FLASH Purification ADVANCE THE PACE OF YOUR DISCOVERY TO ACHIEVE THE RESULTS OF TOMORROW PLC Purification Systems Accelerate the pace of your discovery S ave time by minimizing the number of fractions
More informationAgilent Prep LC Columns for Small Molecules and Biomolecules MAINTAIN RAPID, RELIABLE SEPARATIONS AS YOU SCALE-UP
Agilent Prep LC Columns for Small Molecules and Biomolecules MAINTAIN RAPID, RELIABLE SEPARATIONS AS YOU SCALE-UP AGILENT PREP COLUMNS FOR HPLC FLEXIBLE, COST-EFFECTIVE OPTIONS FOR SCALING AND PREPARATIVE
More informationCustomized systems to meet your lab s needs
WATERS PURIFICATION SYSTEMS Compound purification and isolation can be more efficient and more productive With Waters systems, purify using multiple detection choices: n Ultraviolet/Visible (UV/Vis) Absorbance
More informationBÜCHI Labortechnik AG
BÜCHI Labortechnik AG Purification BUCHI Chromatography History 1977: BUCHI Fraction Collector 1978: 1 st preparative chromatography system 2008: automated chromatography system 2014: automated high performance
More informationSolutions Guide. MX Series II Modular Automation for Nano and Analytical Scale HPLC And Low Pressure Fluid Switching Applications
Solutions Guide MX Series II Modular Automation for Nano and Analytical Scale HPLC And Low Pressure Fluid Switching Applications Page 1 of 12 Table of Contents Sample Injection... 3 Two- Selection... 4
More informationSFC Säulen für analytische und preparative Anwendungen
SFC Säulen für analytische und preparative Anwendungen Technology Symposium Vienna, 25 th October 2016 DI Verena Schmid 2016 Waters Corporation 1 CHIRAL SEPARATIONS ACQUITY UPC 2 Trefoil 2016 Waters Corporation
More informationStrengths of Instrumentation Division, IIIM, Jammu
Strengths of Instrumentation Division, IIIM, Jammu Quality & Reliability Skill / Technologies Complete analytical services available in-house Availability of large number of qualified experts Strict adherence
More informationMethod Development of a 2D LC-HRMS Extraction and Detection Method for Organophosphorus Flame Retardants in Environmental Water Samples
Method Development of a 2D LC-HRMS Extraction and Detection Method for Organophosphorus Flame Retardants in Environmental Water Samples Ken Rosnack 1 Lauren Mullin 1,2, Malorie Mella 1, Claude Mallet 1,
More informationApplication Note. Author. Abstract. Pharmaceuticals. Detlef Wilhelm ANATOX GmbH & Co. KG. Fuerstenwalde, Germany mau
Development, validation, and comparison of an HPLC method to analyze paracetamol and related impurities according to the European Pharmacopoeia (EP) and USP using the Agilent 1120 Compact LC and the Agilent
More informationACQUITY Arc VERSATILITY WITHOUT COMPROMISE
ACQUITY Arc VERSATILITY WITHOUT COMPROMISE BUILT UPON A FOUNDATION OF RELIABILITY With the ACQUITY Arc System, analytical scientists can experience true plug-and-play method compatibility for HPLC and
More informationHichrom Limited 1 The Markham Centre, Station Road, Theale, Reading, Berkshire, RG7 4PE, UK Tel: +44 (0) Fax: +44 (0)
Hichrom Limited 1 The Markham Centre, Station Road, Theale, Reading, Berkshire, RG7 4PE, UK Tel: +44 (0)118 930 3660 Fax: +44 (0)118 932 3484 Email: www.hichrom.co.uk Table of Contents Sample Injection...
More informationSMB-Chromatography. basic principle. Batch Chromatography. salad. True Moving Bed (TMB) salad. Chromatography Workshop 03/06 (Poland)
SMB-Chromatography basic principle Batch Chromatography salad True Moving Bed (TMB) salad Merck KGaA Darmstadt/Germany Page 53 SMB-Chromatography basic principle extract (A) feed raffinate (B) fluid solid
More informationThe Use of Preparative Supercritical Fluid Chromatography for the Isolation of Cannabidiolic Acid (CBDA) From Hemp Extract
The Use of Preparative Supercritical Fluid Chromatography for the Isolation of Cannabidiolic Acid (CBDA) From Hemp Extract Andrew Aubin and John Flynn Waters Corporation, Milford MA, USA #emeraldconference
More informationAgilent 218 Purification System. Purify your way
Agilent 218 Purification System Purify your way 218 AGILENT 218 PURIFICATION SYSTEM PURIFY YOUR WAY WITH A FLEXIBLE SYSTEM WITHIN REACH OF YOUR BUDGET The Agilent 218 Purification System has the flexibility
More informationACQUITY Arc TM System
ACQUITY Arc TM System Introducing the Newest Member of the Waters LC Portfolio 2015 Waters Corporation 1 Adopting Modern LC Technology in a Global Economy Highly competitive, regulated business environment
More informationPharmaceutical LC/MS Solutions from Agilent Technologies
Pharmaceutical LC/MS Solutions from Agilent Technologies Application Compendium Overview LC/MS plays a key role in the drug discovery and drug development process. Since the introduction of electrospray
More informationFast Preparative Column Liquid Chromatography (PCLC)
Fast Preparative Column Liquid Chromatography (PCLC) Application Note 224 Joan Stevens, Ph.D. and Gary Scharrer Introduction Preparative HPLC is recognized as a prime method for obtaining pure compounds
More informationIs there a Preparative Chromatography Green, Economic and Productive?
Is there a Preparative Chromatography Green, Economic and Productive? Granada, 23 Febrero 2012 Alberto Méndez Applications Specialist alberto_mendez@waters.com 2012 Waters Corporation 1 Why CO 2? CO 2
More informationFast and Comprehensive 2-Dimensional Liquid Chromatography of Block Copolymers through Temperature Gradient Driven Trapping
Fast and Comprehensive 2-Dimensional Liquid Chromatography of Block Copolymers through Temperature Gradient Driven Trapping International Symposium on GPC/SEC and related techniques Bastiaan Staal & Joshua
More informationApplication Note. Authors. Abstract. Introduction. Pharmaceutical
Scalability of Agilent s Across HPLC and UHPLC Instruments Application Note Pharmaceutical Authors Anne E. Mack, William J. Long Agilent Technologies, Inc. 2850 Centerville Road, Wilmington, DE 19808 USA
More informationSimple Techniques for Improving the Isolation of Synthetic Peptides Jo-Ann Jablonski Principal Scientist Waters Corporation
Simple Techniques for Improving the Isolation of Synthetic Peptides Jo-Ann Jablonski Principal Scientist Waters Corporation 2016 Waters Corporation 1 Agenda Background Techniques Scaling a separation Focusing
More informationSelective Extraction And Analysis of Chemical Migrants from Packaging Material using Supercritical Fluids (SFE)
Selective Extraction And Analysis of Chemical Migrants from Packaging Material using Supercritical Fluids (SFE) liver Burt International Symposium on GPC/SEC and Related Techniques 2015 Waters Corporation
More informationQuality-by-Design-Based Method Development Using an Agilent 1290 Infinity II LC
Quality-by-Design-Based Method Development Using an Agilent 129 Infinity II LC An Efficient Method Development Workflow Combined with ISET-mediated Method Transfer Under Waters Empower 3 CDS Control Application
More informationACQUITY UPLC. I-Class PLUS WHAT SEPARATES YOU FROM EVERYONE ELSE
ACQUITY UPLC I-Class PLUS WHAT SEPARATES YOU FROM EVERYONE ELSE COMPLEX CHALLENGES SOLVED Looking for answers to the most complex scientific challenges is what you do. Your research could lead to ground-breaking
More informationFinal Report. Characterisation of Sample Report. Job No 2016/11/12-34 AS No. 1234A. Client Example Contact Sample. Signed. Example report.
Final Report Title Characterisation of Job No 2016/11/12-34 AS No. 1234A Client Contact Sample Author report Signed Date 25 th November 2016 Easy Reach Report 20161124 1 of 30 Contents 1. Study Summary
More informationOverview of preparative HPLC. Analytical Technologies Limited
Overview of preparative HPLC Analytical Technologies Limited 1.Review of liquid phase chromatography origin develop prospect Mobile phase Tewett 1903 Stationa ry phase origin Separation result Liquid-stationary
More informationMass Spectrometry Analysis of Liquid Chromatography Fractions using Ettan LC MS System
GUIDE TO LC MS - December 21 1 Spectrometry Analysis of Liquid Chromatography Fractions using Ettan LC MS System Henrik Wadensten, Inger Salomonsson, Staffan Lindqvist, Staffan Renlund, Amersham Biosciences,
More informationAgilent 1260 Infinity LC & LC/MS Purification Systems. Purify your way
Agilent 1260 Infinity LC & LC/MS Purification Systems Purify your way 1260 AGILENT 1260 INFINITY LC & LC/MS PURIFICATION SYSTEMS PURIFY YOUR WAY WITH MAXIMUM PURITY AND RECOVERY Agilent 1260 Infinity LC
More informationSolubility Studies Utilizing a Phase Monitor
Solubility Studies Utilizing a Phase Monitor Jennifer L. Lefler*, Mansi Bhagdeo** and Manon S. Villeneuve Single Sample Purification: Analytical Sciences, GlaxoSmithKline, Research Triangle Park, NC *
More informationA Modular Preparative HPLC System for the Isolation of Puerarin from Kudzu Root Extracts
A Modular Preparative HPLC System for the Isolation of Puerarin from Kudzu Root Extracts Andrew Aubin and Ronan Cleary Waters Corporation, Milford, MA, USA APPLICATIN BENEFITS A modular preparative HPLC
More informationAutomated Purification Software for Agilent OpenLAB CDS
Automated Purification Software for Agilent OpenLAB CDS Data Sheet Introduction Agilent 1260 Infinity LC and LC/MS purification systems offer flexible and easy to use solutions for purification of microgram
More informationSupporting Information. Identification of N-(2-Phenoxyethyl)imidazo[1,2-a]pyridine- 3-carboxamides as New Anti-tuberculosis Agents
Supporting Information Identification of N-(2-Phenoxyethyl)imidazo[1,2-a]pyridine- 3-carboxamides as New Anti-tuberculosis Agents Zhaoyang Wu, Yu Lu, Linhu Li, Rui Zhao, Bin Wang, Kai Lv, Mingliang Liu,
More informationBiotherapeutic Method Development Guide
Biotherapeutic Method Development Guide HALO BIOCLASS 1000 Å PROTEIN SELECTIVITY KIT Fused-Core Particle Technology BIOCLASS Strategy for Optimizing Protein Separations Using Reversed-phase Liquid Chromatography
More informationTechniques for Improving the Efficiency of Large Volume Loading in Preparative Liquid Chromatography
Techniques for Improving the Efficiency of Large Volume Loading in Preparative Liquid Chromatography Jo-Ann M. Jablonski, Andrew J. Aubin, and Wendy Harrop Waters Corporation, Milford, MA, USA APPLICATION
More information[ PURIFICATION SOLUTIONS ] Pure at Heart
[ PURIFICATION SOLUTIONS ] Pure at Heart Purification is at the heart of laboratory analysis. It s the foundation of every lab analysis, whether focused on pharmaceutical/ life science, chemical materials,
More informationA Comparison of Compendia Normal Phase LC Methods Run Under Supercritical Fluid Chromatography Conditions
A Comparison of Compendia Normal Phase LC Methods Run Under Supercritical Fluid Chromatography Conditions Stéphane Dubant Waters ESAC April 5th 2011 2011 Waters Corporation 1 Agenda Introduction and Benefits
More informationGenotoxicity is the property of a compound
Impurities Analysis in Pharmaceuticals: Genotoxicity is the property of a compound known to have irreversible effects on the structure and functionality of the DNA in cells and cause DNA loss, DNA replication
More informationBiotherapeutic Method Development Guide
Biotherapeutic Method Development Guide HALO BIOCLASS 1000 Å PROTEIN SELECTIVITY KIT Fused-Core Particle Technology BIOCLASS Strategy for Optimizing Protein Separations Using Reversed-phase Liquid Chromatography
More informationChemical constituents of the mangrove-associated fungus Capnodium
SUPPLEMENTARY MATERIAL Chemical constituents of the mangrove-associated fungus Capnodium sp. SZ-F22. A new eremophilane sesquiterpene Haibing He a, Zhongjun Ma a*, Qianqian Wang a, Yu Liu b* and Hualin
More informationChem 321 Lecture 23 - Liquid Chromatography 11/19/13
Chem 321 Lecture 23 - Liquid Chromatography 11/19/13 Student Learning Objectives High Performance Liquid Chromatography With the advent of relatively inexpensive and reliable pumps, the development of
More informationITSP Extraction of Cortisol from Plasma
ITSP Extraction of Cortisol from Plasma Introduction Solid Phase Extraction (SPE) is well established as a preferred tool for extracting small organic molecules from complex biological matrices (e.g. plasma)
More informationA Comprehensive Workflow to Optimize and Execute Protein Aggregate Studies
A Comprehensive Workflow to Optimize and Execute Protein Aggregate Studies Combining Size Exclusion Chromatography with Method Development and Light Scattering Application Note Biotherapeutics and Biosimilars
More informationAnalysis of amoxicillin and five impurities on the Agilent 1220 Infinity LC System
Analysis of amoxicillin and five impurities on the Agilent Infinity LC System LC analysis of impurities down to the.% level with long sub--µm columns, high flow rates and back pressure greater than bar
More informationDisulfide Linkage Analysis of IgG1 using an Agilent 1260 Infinity Bio inert LC System with an Agilent ZORBAX RRHD Diphenyl sub 2 µm Column
Disulfide Linkage Analysis of IgG1 using an Agilent 126 Infinity Bio inert LC System with an Agilent ZORBAX RRHD Diphenyl sub 2 µm Column Application Note Biotherapeutics & Biosimilars Author M. Sundaram
More informationENVIRONMENTAL ANALYSIS. Solutions for Your Analytical Business Markets and Applications Programs. Solution Note
ENVIRONMENTAL ANALYSIS A TURN-KEY GUARANTEED ANALYSER FOR THE ROUTINE MEASUREMENT OF GLYPHOSATE AND AMPA IN DRINKING WATER (EUROPEAN DRINKING WATER DIRECTIVE 98/83/EC) AND OTHER ENVIRONMENTAL WATER SAMPLES
More informationAnalysis of Nucleosides Using an Agilent Infinity II High Speed UHPLC with the 6130 Single Quadrupole Mass Selective Detector
Analysis of Nucleosides Using an Agilent Infinity II High Speed UHPLC with the 6130 Single Quadrupole Mass Selective Detector Application Note Clinical Research Author Patrick Cronan Agilent Technologies,
More informationAutomatic Combination of Orthogonal Separation Modes for Multiple Components in Mixtures
Automatic Combination of Orthogonal Separation Modes for Multiple Components in Mixtures Thomas E. Wheat, Amanda B. Dlugasch, and Patricia R. McConville Waters Corporation IFPAC January 26, 2016 2016 Waters
More informationThe Agilent 1260 Infinity BioInert Quaternary Pump. Scope of a low-pressure mixing UHPLC pump with Bio-Inert Capabilities
The Agilent 1260 Infinity BioInert Quaternary Pump Scope of a low-pressure mixing UHPLC pump with Bio-Inert Capabilities Patrick Cronan Applications Scientist Agilent Technologies Boston, MA 1 Comparison
More informationNanoLC-Ultra system. data sheet. Introducing the NanoLC-Ultra family of high pressure HPLCs for proteomics research
data sheet NanoLC-Ultra system The new NanoLC-Ultra is Eksigent s third generation system, delivering superior gradient precision at pressures up to 10,000 psi. Introducing the NanoLC-Ultra family of high
More informationBenefits of 2D-LC/MS/MS in Pharmaceutical Bioanalytics
Application Note Small Molecule Pharmaceuticals Benefits of 2D-LC/MS/MS in Pharmaceutical Bioanalytics Avoiding Matrix Effects Increasing Detection Sensitivity Authors Jonas Dinser Daiichi-Sankyo Europe
More informationOptimizing Purified Sample Recovery on Reverse-Phase HPLC Systems
Optimizing Purified Sample Recovery on Reverse-Phase HPLC Systems Application Note 209 Joan Stevens, PhD, Alan Hamstra, Luke Roenneburg, Tim Hegeman (Gilson, Inc.) Craig Esser, PhD, Regina Black, Derek
More informationPurification, Purity Analysis, and Impurity Analysis Solutions. for Pharmaceutical Research and Development
Purification, Purity Analysis, and Impurity Analysis Solutions for Pharmaceutical Research and Development Realize Effective Pharmaceuticals The current environment within the pharmaceutical industry an
More informationHigh-Throughput LC/MS Purification of Pharmaceutical Impurities
High-Throughput LC/MS Purification of Pharmaceutical Impurities Application Note Small Molecule Pharmaceuticals Author Florian Rieck Agilent Technologies, Inc. Waldbronn, Germany Abstract Legal regulations
More informationDiscovery BIO Wide Pore
Discovery BIO Wide Pore Solutions to Protein and Peptide Separation Challenges T403118 2 Agenda: What is Discovery BIO Wide Pore Physical characteristics Why we developed it and for whom Performance demonstrations
More informationMartin Gilar Waters Corporation, Milford, MA, U.S. Origins of synthetic oligonucleotides impurities. Lab-scale isolation options
LIGNUCLETIDE SEPARATIN TECHNLGY: SYNTHESIS CHALLENGES AND HPLC ISLATIN PTINS Martin Gilar Waters Corporation, Milford, MA, U.S. INTRDUCTIN rigins of synthetic oligonucleotides impurities Use of synthetic
More informationPreparative HPLC: Factors and Parameters that Directly Affect Recovery of Collected Fractions
Preparative HPLC: Factors and Parameters that Directly Affect Recovery of Collected Fractions Gary Scharrer and Joan Stevens, Ph.D. Gilson, Inc Middleton, WI www.gilson.com Abstract Purification of synthetic,
More informationDRUG DISCOVERY & 2 September 2010
LC-MS/MS QUANTITATION IN DRUG DISCOVERY & DEVELOPMENT Ludmila Alexandrova 2 September 2010 1 For personal use only. Please do not reuse or reproduce without the author s permission. 2 Presentation Outline
More informationElute LC series. Innovation with Integrity. UHPLC Precision for MS applications LC-MS
Elute LC series UHPLC Precision for MS applications Innovation with Integrity LC-MS Delivering Precision and Speed for Mass Spectrometry Designed for reproducibility in retention time and peak shape, Elute
More informationScale-up with the Agilent SD-1 Purification System analytical and preparative runs on a single system
Scale-up with the Agilent SD-1 Purification System analytical and preparative runs on a single system Technical Overview Author Absorbance Monitored wavelength: 230 nm Methylparaben Ethylparaben 9.717
More informationTechnical Note CYP Inhibition Assays: Versatility in the ADDA High Speed Dual Arm Autosampling System
Technical Note CYP Inhibition Assays: Versatility in the ADDA High Speed Dual Arm Autosampling System Data provided by Mike West and John Janiszewski, Pfizer Inc., Groton, CT Introduction ADDA System Features:
More informationApplication Solutions. For Preparative-Scale Chromatography
Application Solutions For Preparative-Scale Chromatography APPLICATION SOLUTIONS FOR PREPARATIVE-SCALE CHROMATOGRAPHY 4 5 9 15 21 27 33 39 43 49 53 57 63 69 75 Introduction UV-Directed Purification of
More informationHPLC to UPLC Method Migration: An Overview of Key Considerations and Available Tools
HPLC to UPLC Method Migration: An Overview of Key Considerations and Available Tools Dr. Michael Swartz, Ph. D. Principal Consulting Scientist Worldwide Pharmaceutical Business Operations Waters Corporation
More informationMPLC. Simply Efficient! Watchers Flash Flash Chromatography by ISU. Ver
MPLC Simply Efficient! Watchers Flash Flash Chromatography by ISU Ver. 05-11 MPLC Watchers Flash Cartridges - For performance and simplicity of use - Fast flash technology - Direct dry loading feature
More informationDeveloping Quantitative UPLC Assays with UV
Developing Quantitative UPLC Assays with UV Detection for Antibodies & Other Proteins Steve Taylor 2011 Waters Corporation 1 Outline UPLC technology for RP protein separations Method development parameters
More informationReveleris Technologies
Reveleris Technologies A Full Spectrum of Purification Solutions The purification revolution continues... History of Commitment and Innovation Whether you are engaged in new-entity discovery or large-scale
More information9/2/2014. USP Monograph Modernization. Todays topics. USP basic. Todays topics. - USP basic. - USP publications. - USP monograph modernization
USP Monograph Modernization Procedure Review and Development Donald Min 2 USP basic Since USP's founding in 1820, our operations have grown exponentially: from 11 volunteers collaborating from their respective
More informationMethod Translation in Liquid Chromatography
Method Translation in Liquid Chromatography Technical Overview Abstract Ronald E. Majors Agilent Technologies, Inc. 2850 Centerville Rd Wilmington, DE 19808 USA With the recent emphasis on high performance
More informationMaximizing Sample Recovery on the ACCQPrep HP125
Maximizing Sample Recovery on the ACCQPrep HP125 Chromatography Technical Note TN42 Overview This technical note is to investigate the sample recovery and injection efficiency of different methods of injection
More informationAgilent 1260 Infinity Bio-inert Quaternary LC
Agilent 1260 Infinity Bio-inert Quaternary LC Features, Technical Details, Applications and Infinity Bio-inert Quaternary LC The Agilent 1260 Infinity Bio-inert Quaternary LC system is a dedicated solution
More informationand for the Development of Isolation Strategies
PLC for the nalysis of Synthetic Peptides and for the Development of Isolation Strategies Joomi hn, Ziling Lu, Thomas E. Wheat, and Jeffrey R. Mazzeo Waters Corporation, Milford, M,.S. Introduction Peptides
More informationApplication Note. Author. Abstract. Biopharmaceuticals. Verified for Agilent 1260 Infinity II LC Bio-inert System. Sonja Schneider
Combining small-scale purification and analysis of monoclonal antibodies on one instrument Protein purification with high-volume injection using the Agilent 126 Infinity Bio-inert Quaternary LC System
More informationBivalirudin Purification:
Bivalirudin Purification: Sorbent Screening and Overload Experiments Marc Jacob, Joshua Heng, and Tivadar Farkas Phenomenex, Inc., 411 Madrid Ave., Torrance, CA 90501 USA PO94190412_W Abstract In this
More informationOnline LC Sample Preparation with BioTrap 500 C18
Online LC Sample Preparation with BioTrap 500 C18 Jörgen Hermansson Anders Grahn Inger Hermansson ChromTech AB Stockholm, Sweden Phone: 08-464-8020 Fax: 08-464-7020 E-mail: ct@chromtech.se http://www.chromtech.
More informationGold Standard for recovery and purity
Gold Standard for recovery and purity Agilent 1200 Series Purification Systems Our measure is your success. products applications software services Maximize recovery and purity of your compounds Regardless
More informationPreparative Purification of Corticosteroids by HPLC; Scalability and Loadability Using Agilent Prep C18 HPLC Columns Application
Preparative Purification of Corticosteroids by PLC; Scalability and Loadability Using Agilent Prep C18 PLC Columns Application Pharmaceuticals Authors Cliff Woodward and Ronald Majors Agilent Technologies,
More informationTechnical Overview. Author. Abstract. Edgar Naegele Agilent Technologies, Inc. Waldbronn, Germany
New Features of the Agilent Method Scouting Wizard for Automated Method Development of Complex Samples Analysis of Large Data Sets by Method Scouting Reports and Automated Adjustment of Flow Rates and
More informationPersonal Organic Purification
Personal Organic Purification Optimize gradient at any time during run Purify 5 mg - 33 gm without additional hardware Separate compounds using normal or reversed phase chromatography Monitor and control
More informationAnalytical Instrument Qualification According to USP <1058>: Requirements and Examples for the Agilent 1290 Infinity LC System
Analytical Instrument Qualification According to USP : Requirements and Examples for the Agilent 1290 Infinity LC System 1) USP : Scope, approach, requirements 2) Qualification Examples for
More informationPerformance characteristics of the Agilent 1100 Series capillary LC system using diode-array UV and MS for detection. Technical Note.
Performance characteristics of the Agilent 11 Series capillary LC system using diode-array UV and MS for detection Technical Note Abstract This application has been verified using an Agilent 1 Series LC
More informationAutomated Scouting of Stationary and Mobile Phases Using the Agilent 1290 Infinity II Method Development Solution
Automated Scouting of Stationary and Mobile Phases Using the Agilent 129 Infinity II Method Development Solution Technical Overview Authors Edgar Naegele and Sonja Schneider Agilent Technologies, Inc.
More informationSimplifying Methods Transfer: Novel Tools for Replicating Your Established Methods on an ACQUITY Arc System
Simplifying Methods Transfer: Novel Tools for Replicating Your Established Methods on an ACQUITY Arc System Paula Hong, Richard Andrews, Peyton C. Beals, and Patricia R. McConville Waters Corporation,
More informationIsolation of cis-cinnamoylcocaine from Crude Illicit Cocaine via Alumina Column Chromatography
Technical Note Isolation of cis-cinnamoylcocaine from Crude Illicit Cocaine via Alumina Column Chromatography John F. Casale*, Enrique L. Piñero, and Elizabeth M. Corbeil U.S. Department of Justice Drug
More informationPuzzled About LCMS? Sample. Sensitivity in Mass Spec Analysis. Prep. Adapting LC-UV. Optimizing and Maintaining Your Mass Spec LCMS
Puzzled About LCMS? Sensitivity in Mass Spec Analysis Sample Prep Adapting LC-UV Optimizing and Maintaining Your Mass Spec To LCMS Paul Altiero Alex Ucci Applications Phone Support Chemistry and Supplies
More informationfor Acclaim Carbamate Column
for Acclaim Carbamate Column Product Manual for Acclaim Carbamate Page 1 of 13 Product Manual for Acclaim Carbamate Analytical Columns 5 µm, 4.6 x 250 mm, P/N 072924 3 µm, 4.6 x 150 mm, P/N 072925 3 µm,
More informationHighly GSH-sensitive SN-38 prodrug with OFF-to-ON fluorescence switch as a bifunctional anticancer agent
Electronic Supplementary Material (ESI) for Chemical Communications. This journal is The Royal Society of Chemistry 2018 Electronic Supplementary Information for Highly GSH-sensitive SN-38 prodrug with
More informationAutomated fraction re-analysis does it really make sense? Application. Udo Huber. Abstract
Automated fraction re-analysis does it really make sense? Application Udo Huber mau 3500 Yellow 3000 2500 Red 2000 1500 1000 500 Blue Pink 0 0 1 2 3 4 5 6 7 8 9 min Abstract Today preparative HPLC is the
More informationUpdate to USP <621> Chromatography: What does this mean? USP 37 NF 32 1S Current as of August 2014
Update to USP Chromatography: What does this mean? USP 37 NF 32 1S Current as of August 2014 2015 Waters Corporation 1 What is the USP-NF? The United States Pharmacopeia National Formulary (USP-NF)
More informationHigh Speed, Ultra-High Sensitivity, and Robustness Needed for the Quantitation of Pharmaceuticals in Blood Plasma. Application Note. Authors.
High Speed, Ultra-High Sensitivity, and Robustness Needed for the Quantitation of Pharmaceuticals in Blood Plasma Application Note Drug discovery and development: Drug metabolism and pharmacokinetics (DMPK)
More informationSeparate and Quantify Rituximab Aggregates and Fragments with High-Resolution SEC
Separate and Quantify Rituximab Aggregates and Fragments with High-Resolution SEC The Agilent 126 Infinity Bio-Inert Quaternary LC System and the AdvanceBio SEC 3Å, 2.7 µm Column Application Note Biologics
More informationFUTURE-PROOF SOLUTIONS FOR REGULATED LABORATORIES IN THE FACE OF CHANGING USP <621> GUIDELINES
FUTURE-PROOF SOLUTIONS FOR REGULATED LABORATORIES IN THE FACE OF CHANGING USP GUIDELINES How to use UPLC technology within allowable adjustments to improve QC laboratory throughput For many in the
More informationAgilent 1260 Infinity Purification Systems. Infinitely better purity and recovery
Agilent 1260 Infinity Purification Systems Infinitely better purity and recovery 1 Regardless whether you have nanograms or grams of sample Maximize recovery and purity Preparative HPLC has never been
More informationAntibody Analysis by ESI-TOF LC/MS
Application Note PROTEOMICS METABOLOMICS GENOMICS INFORMATICS GLYILEVALCYSGLUGLNALASERLEUASPARG CYSVALLYSPROLYSPHETYRTHRLEUHISLYS Antibody Analysis by ESI-TOF LC/MS Authors Lorenzo Chen, Merck & Company,
More information