The Smallest Changes Lead to the Most Significant Discoveries
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1 The Smallest Changes Lead to the Most Significant Discoveries The StellARray Gene Expression System When using predefined normalizers, one often ignores small changes in gene expression. The dogma is that those small changes represent noise and are not relevant. In actuality, small changes in expression of key genes can have significant impact on metabolic pathways, and may consequently alter cell behavior. Even housekeeping genes change expression profiles,dependingonthemediaorenvironmentalfactorsto which they are exposed. The simplest way to recognize relevant gene expression changes is the StellARray Gene Expression System. Follow these three steps from database search to publishable results: n GeneSieve Query search a gene or biological pathway to screen 15 million publications and find the best StellARray Product n StellARray qpcr Arrays primers arranged in 96-well and 384-well formats for real time PCR n Global Pattern Recognition (GPR) Analysis Tool analyze data in minutes without housekeeping genes Visit togeta20%discountonyourfirstorderofstellarray GeneExpressionSystemProducts. GeneSieve, StellARray, and Global Pattern Recognition are trademarks of Bar Harbor BioTechnology, Inc.
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3 GE Healthcare Whatman is now part of GE Healthcare. Creating a centre of excellence in separation technology. The considerable expertise of Whatman in the fields of sample preparation and filtration technologies has earned them an outstanding reputation world wide. Whatman has key enabling technologies used in the understanding of gene and protein functions. Combining the skills of the two companies will enable GE Healthcare to create strong added value for customers giving unparalleled support to a wide range of research and lab applications. For more information visit
4 QIAGEN QIAGEN PCR PCR/RT-PCR RNAi mirna * HLA * AssayTech0708S1JP * artus cador SBTexcellerator HLA Sample & Assay Technologies
5 Assay technologies by QIAGEN Detect the essence of life Rely on QIAGEN assay technologies for: Multiplex PCR assays End-point and real-time PCR and RT-PCR RNAi and mirna assays Gene expression assays Pathogen detection assays* HPV testing HLA and haplotyping* AssayTech0907S1WW AssayTech0708S1WW Contact QIAGEN today or visit *artus, cador, EASYartus, GenoVision Olerup, QIAplex, and SBTexcellerator HLA products are not available for clinical use in US and Canada. cador, EASYartus, GenoVision Olerup, QIAplex, SBTexcellerator HLA Kits are for research use only in Europe. Sample & Assay Technologies
6 Someday,researcherswillfullyunravelthegeneticmysteries that define human life. When that day arrives, we hope to have played a part. To learn about scientists making significant discoveries today, visit TODAY COULD BE THE DAY Promega Corporation
7 You re Holding Sustainable Science The pages of this journal are made of recycled fiber. The staff of Science is doing its share to reduce, reuse, and recycle. The paper used in printing the pages of this journal contains reused materials. The additional wood used to create this paper comes from a paper mill participating in the PEFC Council (Programme for the Endorsement of Forest Certification) ensuring the paper is made from a sustainable managed forest. And in the future Science and AAAS will look to do more to advance science and conserve the environment. Sustainable Science, now printed on recycled paper with 30% postconsumer content.
8 LightCycler Real-Time PCR Systems Unleash the Potential of Real-Time PCR B C The introduction of the LightCycler Instrument in 1998 launched a new era in real-time PCR, inspiring novel applications and discovery. The versatile LightCycler 480 System continues this tradition, unleashing the potential of real-time PCR through expanded analysis capabilities with the power to meet evolving research demands. A Figure 1: Confidently detect genetic variation with the powerful combination of LightCycler 480 System analysis modules for: A. Melting Curve Analysis B. Endpoint Genotyping C. High Resolution Melting For general laboratory use. Not for use in diagnostic procedures. This LightCycler 480 Real-Time PCR System is licensed under U.S. Patent 6,814,934 and corresponding claims in its non-u.s. counterparts and one or under more of U.S. Patents Nos. 5,038,852, 5,656,493, 5,333,675, or corresponding claims in their non-u.s. counterparts, for use in life science, by implication or by estoppel under any patent claims or for any other implication. The product is covered in-part by US 5,871,908, co-exclusively licensed from Evotec OAI AG. Parts of the Software used for the LightCycler 480 System are licensed from Idaho Technology Inc., Salt Lake City, UT, USA. LIGHTCYCLER and HRM are trademarks of Roche. Other brands or product names are trademarks of their respective holders Roche Diagnostics GmbH. All rights reserved. Efficiently analyze genes with High Resolution Melting: Apply this publication-proven method to investigate SNPs, degree of methylation, and RNA editing. Detect genetic variation with confidence: Use a powerful combination of three robust and convenient analysis methods to obtain comprehensive results (Figure 1). Rapidly analyze gene expression data: Choose from a broad range of basic and advanced quantification methods to meet your research needs. Explore the possibilities of Real-Time PCR. Visit to learn more about the LightCycler 480 System and register for updates about special events celebrating the 10 th Anniversary of the LightCycler System. Roche Diagnostics GmbH Roche Applied Science Mannheim, Germany
9 LightCycler Real-Time PCR Systems Unleash the Potential of Real-Time PCR B C The introduction of the LightCycler Instrument in 1998 launched a new era in real-time PCR, inspiring novel applications and discovery. The versatile LightCycler 480 System continues this tradition, unleashing the potential of real-time PCR through expanded analysis capabilities with the power to meet evolving research demands. A Figure 1: Confidently detect genetic variation with the powerful combination of LightCycler 480 System analysis modules for: A. Melting Curve Analysis B. Endpoint Genotyping C. High Resolution Melting For general laboratory use. Not for use in diagnostic procedures. This LightCycler 480 Real-Time PCR System is licensed under U.S. Patent 6,814,934 and corresponding claims in its non-u.s. counterparts and one or under more of U.S. Patents Nos. 5,038,852, 5,656,493, 5,333,675, or corresponding claims in their non-u.s. counterparts, for use in life science, by implication or by estoppel under any patent claims or for any other implication. The product is covered in-part by US 5,871,908, co-exclusively licensed from Evotec OAI AG. Parts of the Software used for the LightCycler 480 System are licensed from Idaho Technology Inc., Salt Lake City, UT, USA. LIGHTCYCLER and HRM are trademarks of Roche. Other brands or product names are trademarks of their respective holders Roche Diagnostics. All rights reserved. Efficiently analyze genes with High Resolution Melting: Apply this publication-proven method to investigate SNPs, degree of methylation, and RNA editing. Detect genetic variation with confidence: Use a powerful combination of three robust and convenient analysis methods to obtain comprehensive results (Figure 1). Rapidly analyze gene expression data: Choose from a broad range of basic and advanced quantification methods to meet your research needs. Explore the possibilities of Real-Time PCR. Visit to learn more about the LightCycler 480 System and register for updates about special events celebrating the 10 th Anniversary of the LightCycler System. Roche Diagnostics Roche Applied Science Indianapolis, Indiana
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12 From Climbing to the Top of the Earth to Launching into Orbit Keynote Presentations THE Meeting Place for the Bioprocessing Industry OVER 1500 ATTENDEES 4 Comprehensive Tracks 110 Presentations featuring 40 Case Studies New FDA and Industry Plenary Session Site Tours to Genentech and Irvine Scientific 3 Pre-Conference Workshops September 23-26, 2008 Disneyland Hotel Anaheim, California BioProcess International 2008 brings you inspiration for the future of a streamlined industry with keynote presentations by visionaries who have climbed the highest mountains, surmounted physical challenges to achieve world endurance records, and supported the launch of NASA shuttles and spacecraft. In addition to the keynotes, the program remains fully grounded in nuts and bolts case studies from biopharmaceutical industry speakers that will help you bring back new tips that can improve your processes tomorrow, while you plan for the coming decade s changes. Biosimilars Follow-on Biologics Subsequent Entry Biologics Biogenerics? Robert L. Garnick, Ph.D. Senior Vice President Regulatory, Quality and Compliance Genentech, Inc. Paths to Flexible Regulatory Notification of Large Molecule Life-Cycle Changes John K. Towns, Ph.D. Director Global CMC Regulatory Affairs Eli Lilly and Co. The Challenges and Successes of Creating a Biologics Organization within Pfizer Rick Rutter, Ph.D. Vice President Pharmaceutical Sciences Biologics Pfizer Inc Manufacturing Support Lessons Learned and Trends for the Future Konstantin Konstantinov, Ph.D. Vice President Technology Development Genzyme Corp. Using Knowledge Management for Technical Collaboration across Generations Jeanne Holm, Ph.D. Chief Knowledge Architect NASA Jet Propulsion Laboratory How to Motivate Staff to Implement Lean Manufacturing to Raise the Bottom Line Rob Bryant, MBA Vice President, Quality, Lean/Six Sigma Program Lead Master Blackbelt Computer Sciences Corporation Special Exhibit Hall Keynote Presentation The Treacherous Path to Success in the Biotech Industry Prof. Charles L. Cooney Robert T. Haslam (1911) Professor, Department of Chemical Engineering, and Faculty Director, Deshpande Center of Technological Innovation, MIT Maximize Your Savings by Registering Early Organized by Priority Code B8171SAD
13 From the publishers of Science, Science Signaling, formerly known as Science s STKE, will add original research starting September Each week the journal will publish leading-edge findings in addition to the current features, including Perspectives, Reviews, Protocols, Meeting Reports, Book Reviews, Teaching Resources, and the Database of Cell Signaling. Science Signaling showcases high-impact research in cellular regulation in such fields as molecular biology, development, immunology, neuroscience, microbiology, physiology and medicine, pharmacology, biochemistry, cell biology, bioinformatics, and systems biology. Submit your research that provides new concepts and new understanding of biological signal transduction for consideration. ISSN: Subscribing to Science Signaling ensures that you and your lab have the latest cell signaling resources. From basic science to design of therapeutics, from molecules to networks and systems design, read the best source Science Signaling. Announcing Chief Scientific Editor for Science Signaling Michael B. Yaffe, M.D., Ph.D. Associate Professor, Department of Biology Massachusetts Institute of Technology Submit your paper to: stke.sciencemag.org/about/help/research.dtl
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15 Fashion Breakthrough of the Year Featured on the 2007 Breakthrough of the Year issue of Science, this T-shirt is designed from an annotated gene sequence map of human chromosome 1. It's yours for just $22.50 plus tax (where applicable) and S+H. Photos available online. Visit aaas.org/go/geneshirt to order.
16 AAAS/Science Business Office New Products Focus: Cell/Tissue Liquid Handling Culture Fast Sample Homogenization The Omni Prep homogenizing station streamlines the sample preparation process and removes tedious, time-consuming manual steps. The six-station system offers high throughput and eliminates cross-contamination. It is completely flexible in terms of tube size, sample type, and volume. It includes timer control, variable speed, and variable vertical oscillation to minimize processing time and handle almostanysampletype.plasticprobes,strongenoughtobeusedfromthesoftest sample to difficult-to-homogenize tissues, can be attached to the system along with the more traditional stainless steel tips. The plastic probes are designed for single usetoeliminatesamplecontamination,butcanbecleanedandreusedifdesired. Camlab For information ConcentratorsforProteinPrep The Vivaspin sample concentrators for protein sample preparation offer fast and convenient concentration of biological samples with minimal membrane blocking, recoveries of greater than 95 percent, and no risk of drying out the sample. Vivaspin provides fast, nondenaturing concentration of up to 30 times for volumes from 100 µl to 20 ml.theinstrumentconcentratessamplesbymembrane filtration to a single tube, with the molecular weight cutoff selected by the user. The sample is placed in the upper compartment of the tube, and centrifugation forces solvent through a semipermeable membrane into the lower compartment. This leaves a concentrated sample in the upper chamber. GE Healthcare For information Label-Free Assay Accessory A new automated liquid-handling accessory (LHA) integrates with the Corning Epic System for high throughput label-free assays. The Epic LHA includes a 384-wellpipettor,amicroplatestackerthatcan handle up to 20 lidded 384-wellmicroplatesandanautomatedtip wash station. The liquid-handling system is designed to maintain thermal stability to eliminate artifacts from temperature fluctuations. TheEpicSystemisahighthroughputlabel-freedrugdiscoverysystem that features patented optical biosensor technology to perform biochemicalandcell-basedassays.thesystemissensitiveenoughto measure the cell response from endogenous receptor expression on primary or nonengineered cell lines, along with protein/drug direct binding events in biochemical assays. Corning For information Safe Vacuum System The Vacusafe system was developed for safe, easy, and rapid handling ofhazardousliquids.thenewgenerationvacusafeisdesignedfor aspiration,disposal,orfiltrationofbiologicalandchemicalliquidsina cleanandprofessionalway.allrequiredfunctionalityisintegratedina compact design that can be used at the push of a button. For aspiration anddisposalapplications,thevacusafecanreplacenoisy,complicated home made systems or systems connected directly to the laboratory vacuum system that risk contamination of the entire vacuum system. Integra Biosciences For information LIFE SCIENCE TECHNOLOGIES Multilabel Plate Reader The Victor X Multilabel Plate Reader offers the flexibility to support new applications beyond primary screening, including quality control and therapeutic research. It includes an enhanced software package that features an improved Start Wizard for easier creation of new protocols, application-based example protocols for off-the-shelf use, andimprovedkineticssupportformonitoringcellularandenzyme assays in real time. The Victor X units are also designed to be easy to upgrade with new accessories. PerkinElmer For information µl Disposable Tip A 50-µl size is a new addition to a line of conductive, disposable tips that include sizes of 10-µl, 200-µl, and 1,000-µl. The tips fit Tecan s automated liquid-handling workstations, enhancing performance and minimizing cross-contamination in a broad range of genomics, forensics, and enzyme-linked immunosorbent assay (ELISA) applications. The 50-µl tips are optimal for polymerase chain reaction set-up, DNA quantification and normalization, and small-volume applications such as ELISAs. Tecan For information Nanoliter Dispensing Tips A new line of PocketTips enables nanoliter-volume dispensing on laboratory automated systems. An internally molded capillary pocket allows transfer of 50-nl to 500-nl molecular compounds suspended in dimethyl sulfoxide (DMSO) directly to assay plates. This in-tip mixing savestime,reagents,andlabwareaswellasimprovingdataquality. PocketTips are made from high-quality polypropylene that eliminates adherenceandmaximizestherecoveryoffluids.throughaspiration, the capillary pocket fills with the specified amount of compound solution and facilitates soft in-tip mixing of sample and assay material. By removing intermediate dilution steps, this innovative pipetting method makes it possible to perform assays more quickly, conserve valuable compounds, and prevent compound precipitation for more accurateandpreciseresults.thetipsaresuitableforallsecondary compound screening applications, such as selectivity assays, potency determinations, cell- and enzyme-based assays, and polymerase chain reaction primer dilutions. Thermo Fisher For information Electronically submit your new product description or product literature information! Go to for more information. Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this space. Emphasis is given to purpose, chief characteristics, and availabilty of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not implied. Additional information may be obtained from the manufacturer or supplier. SCIENCE VOL JULY
17 What a site to see. We veredesignedourwebsitetomake ordering and shopping much faster, easier and more convenient. Come visit us at pipetman.com to see for yourself. Pipettes Recognized worldwide as the gold standard in pipettes, our product line continues to grow and improve to advance your research abilities. From the classic Pipetman and now the new Pipetman Neo with lower spring forces, to digital and motorized pipettes that give you greater control and expanded functionality, you ll find many options to choose from. Tips Gilson s Diamond tips ensure accuracy, precision and purity for trustworthy results. Our Diamond tips cover a full range of volumes and are offered in many convenient quantity and packaging options to best fit the needs of your laboratory. Service Our new Gilson Service Center of Excellence and our new field service team are now available to serve you. From calibration to repair, using only genuine Gilson replacement parts, we ensure your pipettes always perform like new. Special Offer Place your order at pipetman.com and receive 5% off of your entire purchase! Offer expires 08/30/2008. Available only to US customers. Offer may be combined with other discounts or offers. Promotion code M785S must be used at the time order is placed. ti ervice pi tt GILSON O R D E R DIR EC T Gilson, Inc. PO Box Middleton, WI 53562, USA Tel: Fax:
18 Stain DNA, not nature. How can you protect yourself, the environment, and your DNA sample? Answer: SYBR Safe. Fact is, it s just as effective as ethidium bromide, yet it s not a hazardous agent. So it s not only better for the environment, but better for you. And since SYBR Safe uses blue light for visualization, your DNA sample won t be damaged by UV, so you ll get better cloning efficiencies. Next time you re staining DNA, be sure to make the safer choice for yourself and the environment. Get SYBR Safe Invitrogen Corporation. All rights reserved. These products may be covered by one or more Limited Use Label Licenses (see the Invitrogen catalog or our website,
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