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1 Think of Highest Functionality Compose Your Own Personal Scope Listen to the Sound of Science. Discover
2 A new online source of trusted techniques in molecular and cellular biology Contains cutting-edge and classic protocols presented step-by-step with cautions and troubleshooting Frequently updated and annotated Interactive, customizable, and fully searchable Subject Coverage Antibodies Bioinformatics/Genomics Cell Biology Chromatography Computational Biology DNA Delivery/Gene Transfer Electrophoresis Genetics High Throughput Analysis Imaging/Microscopy Immunology Laboratory Organisms Molecular Biology Neuroscience Newly Added Protocols Plant Biology Polymerase Chain Reaction (PCR) Proteins and Proteomics RNA Interference (RNAi)/siRNA Stem Cells Transgenic Technology Cold Spring Harbor Laboratory is renowned for its teaching of biomedical research techniques. For decades, participants in its celebrated, hands-on courses and users of its laboratory manuals have gained access to the most authoritative and reliable methods in molecular and cellular biology. Now that access has moved online. Visit Cold Spring Harbor Protocols today and discover a rich, interactive source of new and classic research techniques. The site is fully searchable, with many tools that can be customized by users, including topic-based alerting and personal folders. Through a web-based editorial process, users also have the opportunity to add refereed comments to each protocol. Links in the online protocols offer additional resources, and step-by-step instructions print out in a convenient form, complete with materials, cautions, and troubleshooting advice. Each protocol is citable and presented and edited in the style that has made Molecular Cloning, Antibodies, Cells, and many other Cold Spring Harbor manuals essential to the work of scientists worldwide. The current collection of more than 1000 protocols is continuously expanded, updated, and annotated by the originators and users of the techniques. CSH Protocols is created by Cold Spring Harbor Laboratory Press in association with HighWire Press of Stanford University. Cold Spring Harbor Protocols Executive Editor: Dr. David Crotty ISSN / online, monthly Available exclusively via institutional site license For pricing information or to request a free trial, visit the website or: Phone: (Continental US and Canada) or (all other locations) Fax: cshpress@cshl.edu or Website: Write: Cold Spring Harbor Laboratory Press, 500 Sunnyside Blvd., Woodbury, NY
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4 LightCycler Real-Time PCR Systems Unleash the Potential of Real-Time PCR A B C The introduction of the LightCycler Instrument in 1998 launched a new era in real-time PCR, inspiring novel applications and discovery. The versatile LightCycler 480 System continues this tradition, unleashing the potential of real-time PCR through expanded analysis capabilities with the power to meet evolving research demands. Efficiently analyze genes with High Resolution Melting: Apply this publication-proven method to investigate SNPs, degree of methylation, and RNA editing. Figure 1: Confidently detect genetic variation with the powerful combination of LightCycler 480 System analysis modules for: A. Melting Curve Analysis B. Endpoint Genotyping C. High Resolution Melting For general laboratory use. Not for use in diagnostic procedures. This LightCycler 480 Real-Time PCR System is licensed under U.S. Patent 6,814,934 and corresponding claims in its non-u.s. counterparts and one or under more of U.S. Patents Nos. 5,038,852, 5,656,493, 5,333,675, or corresponding claims in their non-u.s. counterparts, for use in life science, by implication or by estoppel under any patent claims or for any other implication. The product is covered in-part by US 5,871,908, co-exclusively licensed from Evotec OAI AG. Parts of the Software used for the LightCycler 480 System are licensed from Idaho Technology Inc., Salt Lake City, UT, USA. LIGHTCYCLER and HRM are trademarks of Roche. Other brands or product names are trademarks of their respective holders Roche Diagnostics GmbH. All rights reserved. Detect genetic variation with confidence: Use a powerful combination of three robust and convenient analysis methods to obtain comprehensive results (Figure 1). Rapidly analyze gene expression data: Choose from a broad range of basic and advanced quantification methods to meet your research needs. Explore the possibilities of Real-Time PCR. Visit to learn more about the LightCycler 480 System and register for updates about special events celebrating the 10 th Anniversary of the LightCycler System. Roche Diagnostics GmbH Roche Applied Science Mannheim, Germany
5 Gordon Research Conferences GRC is holding over 190 meetings in A few upcoming meetings that may be of particular interest to Genes & Development readers are listed below. Signaling by Adhesion Receptors (Graduate Research Seminar) NEW! June 28-29, 2008 Mount Holyoke College, South Hadley, MA Signaling by Adhesion Receptors (GRC) June 29 - July 4, 2008 Mount Holyoke College, South Hadley, MA The Biology of Post-Transcriptional Gene Regulation June 29 - July 4, 2008 Colby College, Waterville, ME Signal Transduction by Engineered Extracellular Matrices July 6-11, 2008 Bates College, Lewiston, ME Plant Molecular Biology July 13-18, 2008 Holderness School, Holderness, NH Macromolecular Organization & Cell Function July 27 - August 1, 2008 Magdalen College, Oxford, United Kingdom Growth Factors & Signalling August 3-8, 2008 Magdalen College, Oxford, United Kingdom Immunochemistry & Immunobiology August 17-22, 2008 Magdalen College, Oxford, United Kingdom 75 years at the frontiers of science:
6 4th Edition By John A. Kiernan The chemical and physical principles of fixation, staining, and histochemistry in one volume! Now in its fourth edition, Histological and Histochemical Methods has been expanded and updated with the latest techniques and developments within the field, whilst retaining the details of the classic techniques still in use. The relations of chemical structures and reactions to fixation, tissue processing, staining, enzyme location, immunohistochemistry and other procedures are explained in simple, practical terms. This book is recommended for lecturers and students in biological and biomedical disciplines using histological and histochemical techniques. Postgraduate researchers will find this to be the ideal laboratory companion, as will medical laboratory technologists preparing for their professional examinations. Every histopathology laboratory should have a copy. 2008, 606 pp., Bibliography, Glossary, Index Paperback: $75.00 ISBN CONTENTS 1. Introduction to microtechnique 2. Fixation 3. Decalcification and other treatments for hard tissues 4. Processing and mounting 5. Dyes 6. Histological staining in one or two colours 7. Staining blood and other cell suspensions 8. Methods for connective tissue 9. Methods for nucleic acids 10. Organic functional groups and protein histochemistry 11. Carbohydrate histochemistry 12. Lipids 13. Methods for inorganic ions 14. Enzyme histochemistry: general considerations 15. Hydrolytic enzymes 16. Oxidoreductases 17. Methods for soluble organic compounds of low molecular weight 18. Metal reduction and precipitation methods 19. Immunohistochemistry 20. Miscellaneous data Bibliography Glossary Index To order or request additional information: Call: (Continental US and Canada) Fax: USorders@scionpublishing.com WWW Site: Write: Scion Publishing Ltd., 500 Sunnyside Blvd., Woodbury, NY
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