International Food Biotechnology Committee - IFBiC

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1 International Workshop on Harmonisation of GMO Detection and Analysis for Central and South America Foz do Iguazu, Brazil December 2009 ILSI International Food Biotechnology Committee - IFBiC The resource for science on agricultural biotechnology.

2 "The ILSI Experience: Advancing Science Underlying GM Sampling and Detection Methods Around the Globe" Elizabeth Hodson de Jaramillo, Ph.D. Profesora Emérita Pontificia Universidad Javeriana

3 Capacity building in biosafety, and articulation of protocols is crucial for biosafety evaluations.... molecular biology is a key tool for scientifically sound evaluations.

4 ILSI is a nonprofit, worldwide organizations whose mission is to improve public health and well-being and to advance scientific understanding in the areas related to nutrition, food safety, risk assessment, and the environment. IFBiC, the International Food Biotechnology Committee is an important source for science on agricultural biotechnology, a global leader in delivering training workshops on sampling and detection of GM products. It identifies gaps in knowledge; share basic science; and provide hands-on training in context of the local and regional needs.

5 ILSI is a neutral forum for discussing and advancing scientific issues that impact public health. Academic Scientists Industry Scientists Government Scientists ILSI is not a trade organization and conducts no lobbying activities. ties. ILSI International Food Biotechnology Committee

6 ILSI is a global network. ILSI Argentina ILSI Brasil ILSI Europe ILSI Focal Point in China ILSI-India India ILSI Japan ILSI Korea ILSI Mexico ILSI North Africa and Gulf Region ILSI North America ILSI North Andean ILSI South Africa ILSI South Andean ILSI Southeast Asia Region indicates ILSI Country or Regional Office ILSI International Food Biotechnology Committee

7 How ILSI accomplishes its mission: Research Workshops and Conferences Publications ILSI International Food Biotechnology Committee

8 ILSI Four Global Issues Functional foods Obesity Risk Assessment Biotechnology ILSI International Food Biotechnology Committee

9 ILSI is a Global network of scientists Partner with FAO, WHO, and other government agencies Source of credible, scientific knowledge providing science for better public health decision-making Visit us on the web: ILSI International Food Biotechnology Committee

10 IFBiC Mission and Objectives Encourage use of sound science in decision making Address key scientific issues by collaborating with international experts Develop and publish scientific underpinnings for the safety assessment of foods and feeds derived from biotech crops Ensure that scientific information regarding the food and feed safety of biotech crops is available to the scientific and regulatory communities Help build global scientific capacity ILSI International Food Biotechnology Committee

11 IFBiC = International Partnerships ILSI International Food Biotechnology Committee

12 Outreach and Publications The key to the success of IFBiC! >30 workshops/symposia organized/funded 16 publications/reports, 1 in process, plus posters Publications recognized and cited by EFSA, Codex countries, OECD, others

13 IFBiC = Publications Marci Levine, mlevine@ilsi.org ILSI International Food Biotechnology Committee

14 IFBiC = Key Scientific Issues Sampling and detection methods Lack of accessible, current baseline composition data Nutritionally improved crops Performance of animals fed biotechnology-derived feeds Protein safety New Technologies RNAi and transcription factors Stacked events combining biotech traits via breeding Role of mammalian toxicology studies in safety assessment ILSI International Food Biotechnology Committee

15 IFBiC = Capacity Building and Harmonization Workshops on what detection methods can and cannot do 16 workshops, 20 countries, 180 students ~ 1200 scientists who develop, assess, implement Demonstrations of critical concepts related to sampling and detection methods Hands-on wet-lab training in DNA/Protein methods Brazil and Argentina, 02, 08; India 2x 03; China 05, 2x 09; Singapore, 08, Colombia 08; Chile 08 >180 students Partners: ILSI Branches, AACC, JRC, FAO/WHO, AVA, UNU-BioLAC Well received, many new & follow-up requests

16 Sampling and Detection Workshops- Topic Areas Agricultural-biotechnology. Local and International perspective How sampling detection methods are used in the actual supply chain: Seeds, field, grain, commodities, food, complexity: matrix, sampling, units, costs. Low level presence, International standards such as Codex, ISO Technical aspects of Sampling and detection. Sampling (seed, grain, processed foods) Protein & DNA-based methods quantitative and qualitative methods, reference materials, etc. Interpretation of results Validation and proficiency testing Design and implementation of a detection laboratory Other topics: Screening methods Approaches to detection of combined events (stacks)

17 ILSI Brasil Workshop Bases Científicas para Avaliação do Risco de OGMs como Alimento Scientific Basis for GM Food Safety Assessment October 13th - 14th, 2008 Brasília, DF - Brazil

18 Beijing 2005

19 Beijing 2005

20 Singapore 2008 Marco Mazzara, Singapore 2008

21 Taller Internacional Métodos de Muestreo y Detección n en Organismos Genéticamente Modificados ILSI Sur-Andino e IFBiC Santiago de Chile Agosto, 2008

22 Shanghai 2009

23 Dr. Zhu Suifan CAIQ Beijing, 2009 Beijing 2009

24 Some examples of subjects analyzed in the workshops.

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26 International Workshop on Detection of Genetically Modified Organisms Bogotá,, Colombia Nov Participants from Latin America (100 theor. 36 Lab)

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28 International Workshop on Detection of Genetically Modified Organisms Bogotá,, Colombia Nov. 2008

29 International Workshop on Detection of Genetically Modified Organisms Bogotá,, Colombia Nov. 2008

30 Protein-based testing in laboratory

31 PCR laboratory

32 Screening methods By Screening Shipment is tested using methods which detect multiple events Typically uses common elements Screening test PCR method for promoter, terminator or Screening process PCR for multiple events ILSI International Food Biotechnology Committee

33 Does 35S or Tnos positive mean GM is present? CaMV is commonly found in Brassicas A A positive result for the CaMV35s 5 5 may indicate the presence of the Cauliflower Mosaic Virus itself and not a GM event. Mewett et al. Maintaining product integrity in the Australian seed and grain supply chain the role of sampling and testing for GM events Australian NMI Brassica (cauliflower ) is commonly used as a thickener in soups (Colombia, and others). ILSI International Food Biotechnology Committee

34 Sharing knowledge and updating researchers and practitioners through the workshops has been a very valuable input for capacity building in Biosafety

35 Examples of international cooperation for harmonisation in scientific risk evaluation BioLAC GEF-WB Biosafety projects (Brazil, Colombia, Costa Rica, Perú): Multi-Country Capacity-Building for Compliance with the Cartagena Protocol on Biosafety Regional technical & scientific capacities Communication & Public Perception

36 Thank you!! ILSI / IFBiC contacts: mlevine@ilsi.org