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1 The University of Jordan Faculty: Pharmacy Department: Pharmaceutics & Pharmaceutical technology Program: Pharmacy & Pharm D Academic Year/ Semester: 2013/2014 Pharmaceutical Microbiology II (Course Number: ) Credit hours 2 Level 4 th yr Prerequisite Pharmaceutical microbiology I Coordinator/ Lecturer Course website Office number Office phone Place Office hours Day/Time Sunday Monday Tuesday Wednesday Thursday Course Description In this course the students will apply some of the knowledge they gained in pharmaceutical microbiology I & pharmaceutical microbiology II. The students will perform tests used to monitor the environment (air, personnel, water) & test sterile products. Also the students will perform microbial identification through gram staining. The students will perform various in vitro tests for evaluating antimicrobial agents & will perform some microbial quality tests for non sterile products. Learning Objectives By the end of this practical sessions the student will develop the skills of identifying microorganisms, measuring the efficacy and potency of different antimicrobial agents, using different sterilization methods and designing optimum sterilisation cycles and monitoring microbiological quality for both sterile and non sterile dosage forms. Intended Learning Outcomes (ILOs): Successful completion of the course should lead to the following outcomes: Page 1
2 A. Knowledge and Understanding: Student is expected to A.1 Know the general laboratory safety awareness practical skills A.2 Know the different qualitative and quantitative tests that are used to A.3 Evaluate microbial susceptibility towards different antimicrobial agents A.4 Know the different techniques that are used to monitor microbial quality of the environment and pharmaceutical preparations A.5 Know the different methods that are used to control microbial A.6 Know the different methods used to identify microorganisms A.7 Know the different methods used to culture microorganisms and the different media used B. Intellectual, Analytical and Cognitive Skills: Student is expected to B.1 Calculate and interpret the MIC of different antimicrobial agents B.3 Judge the microbial quality of different pharmaceutical preparation and environmental conditions B.4 Calculate D value, Z- value and the lethality of different sterilization cycles B.5 Identify microorganisms macroscopically and microscopically B.6 Quantifying microbial C. Subject-Specific Skills: Student is expected to C.1 Practical skills of aseptic techniques C.2 Practical skills of handling microbial culture C.3 Practical skills of identifying and quantifying bacterial culture C.4 Practical skills of designing sterilization cycle C.5 Practical skills of performing different susceptibility tests D. Transferable Key Skills: Students is expected to D.1. Team work D.2. Time management D.3. Data collection, presentation and interpretation D.4. written and oral communication D.5. Analysis D.6. Information data collection ILOs: Learning and Evaluation Methods ILO/s Learning Methods Evaluation Methods Demonstration Experiments Discussion Tutorial Reports Quizzes Practical exams Written exams Page 2
3 Course Contents Content Reference Week ILO/s Practical One Aseptic techniques and subculturing of bacterial cultures Practical Two Sources of microbial Practical three Gram staining and morphology identification of Gram positive, Gram negative and Yeast Qualitative methods used for the evaluation of bacteriostatic activity of different antimicrobial agents Practical four Determination of the minimal inhibitory concentration (MIC) of a bacteriostatic substance by agar diffusion method Practical five Determination of the minimal inhibitory concentration (MIC) using broth dilution method and two serial dilution technique Determination of the potency of an antibiotic solution by the cup plate method Mid term exam Determination of the concentration exponent (η) for different antimicrobial agents Determination of phenol coefficient using Chick 2 Importance of aseptic techniques to prevent 3 To know the practical methods to monitor environmental 4 Identification of microorganisms through staining How to evaluate a biocide qualitatively 5 How to evaluate a biocide quantitatively 6 How to evaluate a biocide quantitatively 7 To learn a method for determining biocide potency 8 Study the effect of dilution on disinfectant activity 9 Page 3
4 Martin Methods Determination of microbial count for water soluble non sterile solid product and water soluble non sterile semi solid product The efficiency of autoclaving as a sterilization method Tutorial "Data handling and interpretation associated with heat sterilization processes" 10 To understand pharmacopeial methods used to evaluate microbial quality of non sterile products 11 To understand the efficiency of autoclaving as sterilizing method 12 To be able to calculate lethal effect of autoclaving cycle Learning Methodology Demonstration Experiments Discussion Tutorial Evaluation Evaluation Point % Date Reports 10 Quizzes 10 Midterm Practical theoretical Final Practical Theoretical Main Reference/s: Page 4
5 Hugo, W.B and Russell, A.D. Pharmaceutical Microbiology. Eighth Edition References: Page 5
The University of Jordan
The University of Jordan Faculty: Pharmacy Department: Pharmaceutics & Pharmaceutical technology Program: Pharmacy Academic Year/ Semester: 2013/2014 Pharmaceutical Microbiology I (Course Number: 1202341)
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