Microbiology with Laboratory (BIOL 190)

Transcription

1 Microbiology with Laboratory (BIOL 190) 4 credit hours June 5 - August 5, 2017 Professor Anastasia Levitin (Bldg. 215, Office 201) Lecture Schedule: Tuesdays and Thursdays, 10:30am-11:50am (Bldg. 517, Classroom 147) Laboratory Schedule: Tuesdays and Thursdays, 1:00pm-4:00pm (Bldg. 517, Laboratory 169) Office hours: by appointment Pre-requisites: General Biology I and II Text: Willey, J.M., Sherwood, L.M., Woolverton, C.J. Prescott s Microbiology, Tenth Edition (ISBN # ) Lab Manual: Harley, J.P., Laboratory Exercises in Microbiology, Tenth Edition (ISBN # ) The books are available for purchase at the Huntley Bookstore, 175 E. Eighth Street, Claremont. Learning Objectives: After completion of this course, students should: 1. Become familiar with important bacteria, viruses, fungi and parasites, with emphasis on clinical applications 2. Learn pertinent facts concerning the role of microorganisms in disease 3. Understand and be able to perform various lab techniques 4. Develop critical reading skills Grading: Group activity and participation 15% Quizzes 15% Midterm exam 20% Final exam 20% Lab notebooks 10% Lab reports 20% Attendance policy: It is very important that students attend all class and laboratory sessions. A significant portion of the grade for this class is based on class participation. Students will receive a zero class participation score for any unexcused absences. All students are allowed one excused absence so long as Dr. Levitin are informed by in advance. In

2 cases of extended (more than one day) excused absences due to sickness or emergencies, please be sure to inform the instructor and you will be given an opportunity to make up missed work. Students with Disabilities: KGI is committed to provide reasonable and appropriate accommodations in compliance with the Americans with Disabilities Act. Any student who needs academic adjustments or accommodations must discuss these needs with the instructor and must also register and be approved for accommodations each semester with the Student Services. All discussions will be kept confidential. Academic Integrity & Honor Code: KGI is committed to the highest ethical and professional standards of conduct. Ethical conduct is an integral part of KGI s mission of education and research aimed at translating into practice, for the benefit of humanity, the power and potential of the life sciences. The Institute expects all members to observe and exhibit ethical behavior, honesty, integrity, and good judgment regularly in all facets of their work for and their relationships with the Institute. Each member is expected to demonstrate respect for the rights of others and is accountable for his/her actions. To assist students in understanding their responsibilities under the Honor Code, the following is a partial list of conduct pertaining to academic matters, which violate the Honor Code. Prohibited conduct includes, but is not limited to, the following: 1. Plagiarism Knowingly appropriating another's words or ideas and representing them as one's own Use of another's ideas or words, including paraphrasing, without acknowledging the source Professor may use Turnitin plagiarism detection software for assignments 2. Provide false information, data, results, or any other misrepresentation of work 3. Cheating Unauthorized collaboration on assignments Unauthorized sharing of information about examination content or questions Use of previously submitted coursework for alternate purposes without prior approval Use of any unauthorized materials or electronic sources for exams or assignments 4. Impeding the learning of others, including but not limited to interrupting another student s exam, requesting unauthorized information, or taking credit for another student s work Reported violations of the Honor Code will be investigated. Failure to follow the Honor Code will carry sanctions which may include, an F for the assignment, exam or course. Impeding investigation, providing false statements, or failure to report violations are considered violations of the Honor Code.

3 Ignorance is NOT an excuse. The student bears the responsibility to learn from the individual instructor the procedure for acknowledging sources and indicating quotations as required for each assignment.

4 Lectures Week # Date Topics Homework (Readings prior to class) Evolution of Microbes (Ch.1) 6-Jun Introduction to Microbiology 1 Microbial Cell Cultures (Ch. 3-6) 8-Jun Microbial Nutrition, Growth, and Control of Microorganisms in the Environment (Ch. 8) 13-Jun Control Antimicrobial Chemotherapy (Ch.9) 2 15-Jun Mechanisms of Genetic Variation (Ch. 16) Microbial Molecular Biology and 20-Jun Recombinant DNA Technology (Ch.17) 3 Genetics 22-Jun Microbial Genomics (Ch. 18) 4 27-Jun Ecology and Symbiosis Microbial Interactions (Ch. 32) 29-Jun Pathogenicity and Host Response Adaptive Immunity (Ch.34) 4-Jul No Class 6-Jul No Class 11-Jul Midterm Exam 5 Ecology and Symbiosis Pathogenicity and Infection (Ch. 35) 13-Jul Pathogenicity and Host Response 18-Jul Clinical Microbiology and Immunology (Ch. 36) 6 20-Jul Epidemiology and Public Health Microbiology (Ch. 37) Microbial Diseases, Detection, and 25-Jul Human Diseases Caused by Viruses and Prions (Ch. 38) 7 Their Control 27-Jul Human Diseases Caused by Bacteria (Ch. 39) 8 1-Aug Human Diseases Caused by Fungi and Protists (Ch. 40) 3-Aug Final Exam

5 Laboratory exercises Week # Lab Topics Lab Manual Ex. 1 (Microscopy), 6-Jun 1 Microscopic techniques and Staining Ex. 7 (Simple Staining) 8-Jun Ex. 8 (Gram Stain) Jun 15-Jun 20-Jun 22-Jun 27-Jul 29-Jul Basic Laboratory and Culture Techniques Medical Microbiology Ex. 13 (Sterilization and media preparation) Ex. 14 (Culture transfer) Ex. 15 (Spreading) Ex. 16 (Streaking) Ex. 17 (Pour plate) Ex. 19 (Bacterial numbers) Ex. 45 (Growth Curve) 4-Jul 6-Jul No Lab 11-Jul Ex. 52 (Hemagglutination reactions) Medical Microbiology 13-Jul Ex. 53 (Isolation of microbiota) 18-Jul Ex. 52 (Hemagglutination reactions) 20-Jul Ex. 53 (Isolation of microbiota) Microbial Genetics and Genomics 25-Aug Transduction 27-Aug Ex. 65 (Isolation and Purification of Bacterial Genomic DNA) 1-Aug Scientific Inquiry Ex. 67 (Case Studies) 3-Aug No lab