Syllabus MCB 301. Laboratory Schedule. Date (pt total) Experiment Lab Manual Pages

Transcription

1 Laboratory Schedule Date (pt total) Experiment Lab Manual Pages DAY 1 Wed Jan 18 Th Jan 19 Course introduction 1: Streak plate 2: Luminescent bacteria I-XXXIX DAY 2 Mon Jan 23 Tu Jan 24 1, 2: Evaluations 3: Spread plate 4: Simple stain 8, DAY 3 Wed Jan 25 Th Jan 26 3: Evaluations 5: Gram stain DAY 4 (12) Mon Jan 30 Tu Jan 31 6: Wet mount, Darkfield and Phase Contrast 7a-d: Enzymes and adaptation, DAY 5 (33) Wed Feb 1 Th Feb 2 7a-d: Enzymes and adaptation, 2 8a: Lactic acid bacteria enrichment DAY 6 (27) Mon Feb 6 Tu Feb 7 8b: Lactic acid bacteria isolation 9: Antibiotic producing bacteria, drying soil DAY 7 (47) Wed Feb 8 Th Feb 9 8c: Lactic acid bacteria observation 9a: Antibiotic producing bacteria enrichment, 10a-b: Environmental effects, DAY 8 (25) Mon Feb 13 Tu Feb 14 9b: Antibiotic producing bacteria purification, 1 10a-b: Environmental effects, 2 10c-d: Environmental effects, II

2 Date (pt total) Experiment Lab Manual Pages DAY 9 (32) Wed Feb 15 Th Feb 16 10c-e: Environmental effects, 2 11a: Sporeformers enrichment DAY 10 (17) Mon Feb 20 Tu Feb 21 11b: Sporeformers isolation, 1 9b: Antibiotic producing bacteria purification, DAY 11 (18) Wed Feb 22 Th Feb 23 9b: Antibiotic producing bacteria purification, 3 11b: Sporeformers isolation, DAY 12 (18) Mon Feb 27 Tu Feb 28 9b: Antibiotic producing bacteria purification, 4 11c: Sporeformers post-enrichment, DAY 13 (30) Wed Mar 1 Th Mar 2 9b: Antibiotic producing bacteria isolation, 1 11c: Sporeformers post-enrichment, 2 13a-b: MIC determination, DAY 14 (17) Mon Mar 6 Tu Mar 7 9c: Antibiotic producing bacteria: determination of antimicrobial spectrum, 1 9d: Antibiotic producing bacteria: preparing frozen stock 9e: Antibiotic producing bacteria: preparing DNA 13a-b: MIC determination, DAY 15 (5) Wed Mar 8 Th Mar 9 9c: Antibiotic producing bacteria: determination of antimicrobial spectrum, 2 9d: Antibiotic producing bacteria: DNA isolation, DAY 16 (5) Mon Mar 13 Tu Mar 14 9c: Antibiotic producing bacteria: determination of antimicrobial spectrum, 3 9d: Antibiotic producing bacteria: DNA isolation, 2 9e: Antibiotic producing bacteria: PCR III

3 Date (pt total) Experiment Lab Manual Pages DAY 17 (5) Wed Mar 15 Th Mar 16 9c: Antibiotic producing bacteria: determination of antimicrobial spectrum, 3 9g: Antibiotic producing bacteria: PCR cleanup, prepare samples for sequencing Sat Mar 18 to Sun Mar 26 DAY 18 (13) Mon Mar 27 Tu Mar 28 Spring Break 9h: Antibiotic producing bacteria: gel electrophoresis 14a: Identification isolation DAY 19 (30) Wed Mar 29 Th Mar 30 14b: Identification purification, 1 15a-c: Biochemical tests, Th Mar 30 DAY 20 (33) Mon Apr 3 Tu Apr 4 OPEN HOURS BEGIN, SEE SCHEDULE 9h: Antibiotic producing bacteria: analyzing DNA sequences 14b: Identification purification, 1 15a-c: Biochemical tests, DAY 21 (21) Wed Apr 5 Th Apr 6 14c: Identification dichotomous keys; inoculate tests, 1 16a: Transposon mutagenesis conjugation DAY 22 (21) Mon Apr 10 Tu Apr 11 14c: Identification; inoculate tests, 2 16c: Transposon mutagenesis selection, IV

4 Date (pt total) Experiment Lab Manual Pages DAY 23 (20) Wed Apr 12 Th Apr 13 14c: Identification; inoculate tests, 3 16c: Transposon mutagenesis selection, Th Apr 13 OPEN HOURS END DAY 24 (12) Mon Apr 17 Tu Apr 18 16c: Transposon mutagenesis characterization, DAY 25 (38) Wed Apr 19 Th Apr 20 16c: Transposon mutagenesis characterization, 2 17a: ELISA fluid exchange 17b: ELISA analysis DAY 26 Mon Apr 24 Tu Apr 25 Lab Practical Review and Practice Lab clean up & drawer inventory DAY 27 (42) Wed Apr 26 Th Apr 27 Lab Practical Exam, Part I Dilution problem calculation, Gram stain, brightfield microscopy, streak plate DAY 28 (58) Mon May 1 Tu May 2 Lab Practical Exam, Part II Dilutions & spread plate, wet mount & phase contrast, biochemical tests, dichotomous key V

5 Lecture Schedule Date January 20 Lecture 1 January 27 Lecture 2 February 3 Lecture 3 February 10 February 17 Lecture 4 February 24 Lecture 5 March 3 Lecture 6 March 10 March 17 Lecture 7 March 24 March 31 Lecture 8 April 7 Lecture 9 April 14 April 21 Lecture 10 April 28 Lecture 11 FINALS WEEK VI Topic(s) Basic microbiology techniques (aseptic technique, pure culture, plating); Luminescent bacteria; Staining (cell wall structure) Microscopy; Enzymes and adaptation Enzymes and adaptation; Lactic acid bacteria Exam 1 Basic microbiology techniques; Luminescent bacteria; Staining; Microscopy; Enzymes and adaptation Lactic acid bacteria; Environmental effects Bacterial Growth; Sporeformers Sporeformers; MIC Exam 2 Lactic acid bacteria; Environmental effects; Bacterial growth DROP DEADLINE Biochemical Tests; Antibiotic producing bacteria Spring Break Biochemical Tests; Antibiotic producing bacteria Transposon mutagenesis; Identification Exam 3 Sporeformers; MIC; Biochemical tests Transposon mutagenesis; ELISA/Agglutination; Dilution problem TBA Final Exam Antibiotic producing bacteria; Transposon mutagenesis; Identification; ELISA/Agglutination

6 Laboratory Assignment Schedule Date Assignment Due Point Total DAY 1 Wed Jan 18 Th Jan 19 DAY 2 Mon Jan 23 Tu Jan 24 DAY 3 Wed Jan 25 Th Jan 26 DAY 4 Mon Jan 30 Tu Jan 31 DAY 5 Wed Feb 1 Th Feb 2 Pre-lab 1-6: Basic Technique Pre-lab 7: Enzymes and adaptation Pre-lab 8: Lactic acid bacteria Post-lab 1-6: Basic Technique DAY 6 Mon Feb 6 Tu Feb 7 Pre-lab 9: Antibiotic producing bacteria Post-lab 7: Enzymes and adaptation DAY 7 Wed Feb 8 Th Feb 9 7: Enzymes & adaptation Lab Report Pre-lab 10: Environmental effects 24 points Fri Feb 10 DAY 8 Mon Feb 13 Tu Feb 14 DAY 9 Wed Feb 15 Th Feb 16 Exam 1 Basic microbiology techniques; Microscopy; Staining; Luminescent bacteria; Enzymes and adaptation Post-lab 8: Lactic acid bacteria Pre-lab 11: Sporeforming bacteria 8: Lactic acid bacteria Lab Report 50 points 65 points VII

7 Date Assignment Due Point Total DAY 10 Mon Feb 20 Tu Feb 21 Post-lab 10: Environmental effects DAY 11 Wed Feb 22 Th Feb 23 10: Environmental effects lab report 60 points Fri Feb 24 12: Growth Curve opens in LON-CAPA at 2pm 41 points DAY 12 Mon Feb 27 Tu Feb 28 DAY 13 Wed Mar 1 Th Mar 2 DAY 14 Mon Mar 6 Tu Mar 7 Pre-lab 13: MIC determination 12: Growth Curve exercise on LON-CAPA Post-lab 11: Sporeforming bacteria 41 points DAY 15 Wed Mar 8 Th Mar 9 11: Sporeforming bacteria Lab Report Post-lab 13: MIC determination 63 points Fri Mar 10 Exam 2 Lactic acid bacteria; Bacterial growth; Environmental effects; 50 points DAY 16 Mon Mar 13 Tu Mar 14 DAY 17 Wed Mar 15 Th Mar 16 13: MIC Lab Report 24 points DAY 18 Mon Mar 27 Tu Mar 28 DAY 19 Wed Mar 29 Th Mar 30 DAY 20 Mon Apr 3 Tu Apr 4 Pre-lab 14: Identification Pre-lab 15: Biochemical Tests VIII

8 Date Assignment Due Point Total DAY 21 Wed Apr 5 Th Apr 6 Pre-lab 16: Transposon mutagenesis Post-lab 9: Antibiotic producing bacteria Post-lab 15: Biochemical tests DAY 22 Mon Apr 10 Tu Apr 11 DAY 23 Wed Apr 12 Th Apr 13 15: Biochemical Tests Lab Report 43 points 9: Antibiotic producing bacteria Lab Report 65 points Fri Apr 14 Exam 3 Sporeformers; MIC; Biochemical Tests 50 points DAY 24 Mon Apr 17 Tu Apr 18 DAY 25 Wed Apr 19 Th Apr 20 Post-lab 14: Identification Pre-lab 17: ELISA 14: Identification Lab Report 47 points DAY 26 Mon Apr 24 Tu Apr 25 16: Transposon mutagenesis Lab Report (NO LATE LAB REPORTS ACCEPTED) 17: ELISA Lab Report (NO LATE LAB REPORTS ACCEPTED) Post-lab 16: Transposon mutagenesis Post-lab 17: ELISA 62 points 26 points DAY 27 Wed Apr 26 Th Apr 27 DAY 28 Mon May 1 Tu May 2 Finals Week Lab Practical Exam, Part I Dilution problem calculation, Gram stain, brightfield microscopy, streak plate Lab Practical Exam, Part II Dilutions & spread plate, wet mount & phase contrast, biochemical tests, dichotomous key Final Exam Antibiotic producing bacteria; Transposon mutagenesis; ELISA/Agglutination; Identification Parts 1 and 2 worth a total of 100 points 50 points IX