I compared the values in this and in the previous reports with the values of vitamin. Experiment (25)

Transcription

1 第3号 栄 養 学 雑 potency its on ( National different dependg to all-trans considered estimation. cause on materials; to be due to reported Institute Nutrition) omeration response depndg bio-potencies are at feedg estimation previous level changg potency th experiment, so it necessary different potencies on report biosay report. results fh are confirmed n I compared ir dose feedg. relationship, th applied to confirm. I compared th previous reports with potency followgs various determations. are experimental s, results albo rats test fh s are already described Results: high level several test albo rats same previous Methods: syntic but re w a trend to 2) experimental natural to compare level. s already described Fally, dose. were Th difference various conditions at ly fh expected s almost comparable w also observed on syntic. under such a difference potency it also necessary In th report, potencies fh whale occured th country w feedg feedg Baba lthough same result w also observed bio-test, rat some m had high potencies ir, it did not show any difference to consider albo, but some had only 70% difference previously fh 1) report, bio-potencies previous 101 Harm s shown 誌 weight crees I determation previous. (25) report. each group per week are shown Table 1 all-trans dcussions. calcurated curves se Fig. are 1 80%

2 Fig. 1. curves defficient albo rats fed critical dose. Table 1. weight cree deficient albo rat. Resulted 80 -Yo II result he I same previous report a little higher Note Each group cont 10 animals. Daily doses are 1 I. U. 21.U. per head, perday. but it different all-trans. But curves weang male rats same stra without any deficient period shown Fig. 2 do not Fig. 2. curves young albo rats cause any difference more five weeks at same level dose m. III rats fed 10 I. U. 20 I. U. per head per day observed used II. se doses correspond to about U. per Kg feed which nearly feedg level per body weight animal or a little lower. s described bee, potency should be expected a feed different true potency. So difference source fh syntic Note: Vitam level same Fig. 1. should be considered

3 第3号 栄 dose. feedg level. I 養 学 compared doses 雑 se fh 誌 Fig fed curves albo dose rats feedg level. group syntic, group natural level limitted to se syntic 70% same levels. result ir about group: at experimental fh are expected th group shown Fig. group h 3 shows a better grow-. apparent calculated per week 126% potency average syntic it weight clearly ment have er trend higher those experi- but mer a little latter natural experiment tion cree IV same. fh used here Notes: average high. u: Fh com- 1: Daily dose 2: Daily dose level paron syntic natural ment h been estimation Samples: bio-potencies previous Method: thier determed per head dose 201. U. u: r=10: 7 U. S. P. Method) experi- purposes 2) country th biosay ( group per head 10 I. U. usg But, derived several fh 3) relationship s carried out here relationship. molecular dtilled fh paper although Embree Results: th se with to confirm because used biosay, ten. group r: Syntic determa- rat number are both. (107, 000 I. U. 109,000 I. U. ). ir already described biosay, 74%. many s were 4) et al used here. ll s th results already are presented were shown Fig. horizontal ax (27) a little 4 where dicates higher vertical. those ax dicates

4 Fig. 4. relationship biopotency ( comparon with result mes) Table 2. amounts bio-potencies (1) Notes: Each group cont 10 albo rats. Daily dose per head 500 I. U. 000 I. U. Total dose per head 1500 I. U I. U. Table 3. amounts (2) *(Yo) RP=101.6-MV x (BB) RP= (MV) (MV)3 (MES) under Fig. 4 w curve simplification correction usg let squares. Notes: Daily dose per head U U. Total does per head U I. U. comparon fh potencies various determations s shown above bio-tests, w a little higher. nd potency fh shown expressed percentage all-trans, Table 4. potencies fh s * pparent

5 Table 5. comparon fh various determations (I. U. /g) Table 6. comparon potencies corrected with those origal we must know th comparg with those spectrophotometries, such 16 th edition U. S. Pharmacopeia, frequently used now commercial transactions. results comparon bio-tests, spectrophotometry antimony trichloride cludg previous paper are shown Table 4 Table 5. Table 6 comparon calculated to obta simplified corrected results author's bio-tests. with those calculated mes' origal. Dcussion In previous report, hio-potencies fh usg mes' methcd were about 70-90%. It w dcussed re th difference due to omeration fh fh not always ferior to syntic some fh samples showed same potencies all-trans. difference level dose w also observed. In th report, experiments albo rats were permed to confirm relationship potency. se bio-tests were a little higher those mes' origal. simplification correction estimation usg se results were tried. re-calculated

6 106 gaed 25yo higher true potency syntic m, a feed actions, tests, Murray fh also terestg marked fresh considered to use. consider s, cause feedg level to be limitted th result at 第20巻 w about not equal all higher true potency considered 16th w. s commercial to spectrophotometry dose. all-trans used supposed actually dose sufficient percentage all-trans range because s terestg expressed a small feedg syntic fresh high omeration h to edition 85%, trans - permed. it U, S. When author's bio- to show true potency when such already durg fh undtilled, extraction s some ly. previous fh dtillation. have all -trans undtilled occured samples et al. even omeration treatments equal mes high dtilled result almost omeration heat to prevent conditions w very especially omeration to get on expected practically extence fact, reports rat. differs th -extracted paper albo previous but 78%. Th result concurs with 5) et al spectrophotometrically shows true general It caluculated U. S. Pharm. all considered on Carr-Price syntic a comparon Pharmacopeia, fh syntic to know its 10% higher fh may be apparent, when but to here fh response important potency 0f w about potency p0tency. s sample. it necessary well lower From fh bio-potencies, will be vestigated who helped me to reported later. I express. also show quatic my gratitude to Ms my appreciation here Oil ssociation Kimiko Okazaki to Japan Vitam Fh Liver Oil Manufacturer's Oil Technical Conference which se experiment. ssociation, co-operated I Japan th study. Summary tests potency albo fh rats, w determed relationship (30) tests

7 第3号 栄 養 学 雑 誌 107 w confirmed. Even lower level needed dose, difference syntic fh w not occured non deficient In feedg syntic level, potency period. potency fh w shown better ir s. By comparon to all-trans various s, relationship spectrophotometry w dcussed. By corrected bio -tests th report, potency w about 10% higher origal, potency fh havg more 78yo equal to w spectrophotometry. References 1) Haruo Baba: Japan. J. Nutr. 2) Ryohei Takada, 3) mes, S. R., W. I. Swanson K. W. Lehman: J. 4) Embree, Goichiro Katsui, N. D., S. R. mes, nalys 4, 59, Interscience 5) Murray, 19, 137 (1961) T. K., R. E. Lehman 馬 Vitam (Japan) m. Pharm. P. L. Harr: 7, 662 (1954) ss. 49, 366 (1960) Method Biochemical Pub. (1957) Campbell, 魚 肝 油 ビ タ ミ ンの Michiko Fukano: J..: Fed. Proc. 20, 453 (1961) 効 力 と し ろ ね ず み に 対 す る 成 長 効 果(要 場 春 旨) 夫(国 立栄 養研 究 所) 前 報 に示 した とお り, 魚 鯨 肝油 ビタ ミンの 生 物効 力 は試 料 に よつ て種 々の値 を示 し, 効 力の大 な ものは 全 トランス型 ビタ ミンに 匹敵 す るが, なか に は相 対 生物 効 力 に して70%を 示 す もの もみ られ た ゆ の よ う な 異 同 は マ レイ ン価 か ら推 定 しえた よ うに, 種 々の条 件 に よっ て生 ず る異 性 化 によ る と考 え られ, ゆの よ うな異 性 化 は合成 ビタ ミンに つ いて も観 察 され た それ らの結果 は しろねず み の 成長 試験 にお い て も認 め ら れ た が, その投 与 量 に よつ て差 異 を生 ず るよ うな傾 向 が み られ た ゆと, 既 往 の実 験値 は合成と 天 然と の効 力 が 投 与 量 の高 い 所 で比 較 され, 且 つ差 異 を生 じてい ない こ とか ら, マ レイ ン価 と生 物効 力 の 関係 を た しか め, 飼 料 レベ ル に近 い投与 量 で の ビタ ミン効 力 の比 較 を行 つ た 又相対 生 物効 力 によつ て ビタ ミンの 効 力 を示 す 場 合, これ は全 トラン ス型 ビタ ミンの 効力 に対 す る%で 表 わ され るの で, 種 々得 られ た値 を用 いて, 一般 測 定 に用 い られ る吸 光法 によ る測 定値 との比 較 検討 を行 つた 以上 の 結果 よ り, 限界 投与 量 にお いて も, 非 欠 乏動 物 で は合成 ビタ ミン油, 天 然 ビ タ ミン油 の ビタ ミン の 生 長 効 力 に差 を生 じな い こ と, 充 分 量 の投 与 では む しろ, 天 然油 が ま さ る ゆと, マ レ イン価 か ら推 定 し た効 力 は, mesら の値 よ り10%程 度 大 きい こと, 相対 生 物効 力78%以 上 の魚肝 油 の ビタ ミン値 は吸 光 分析 値 と一 致 す る ことをみ た (受 付:昭 和37年2月7日) (31)