Center for Mass Spectrometry and Proteomics Phone (612) (612)

Transcription

1 Welcome to the Center for Mass Spectrometry & Proteomics Workshop Department of Biochemistry, Molecular Biology and Biophysics College of Biological Sciences University of Minnesota

2 Welcome to the Center for Mass Spectrometry & Proteomics Workshop University of Minnesota CMSP St Paul CMSP MPLS * * 43 Gortner Laboratory 1479 Gortner Avenue St. Paul, MN maps.google.com MCB 420 Washington Ave. Minneapolis, MN maps.google.com

3 Welcome to the Center for Mass Spectrometry & Proteomics Workshop Department of Biochemistry, Molecular Biology and Biophysics University of Minnesota 43 Gortner Laboratory 1479 Gortner Avenue St. Paul, MN Phone : maps.google.com maps.google.com MCB 420 Washington Ave. Minneapolis, MN Phone :

4 WHO ARE WE? Research Associate

5 Center for Mass Spectrometry and Proteomics

6 OUR CLIENTS Over 52 departments. Customers from over 17 states.

7 WHAT DO WE DO? Support UMN researchers in their proteomic and metabolomic research projects. Project Planning and Management. Train students and other researchers in various aspects of proteomics and metabolomics. Quarterly hands-on workshops. One-Credit Undergraduate Class (January). Participate in research grants along with University Faculty. Emphasis on larger, team-science grants. Generate revenue from internal and external services - to support our operations.

8 DAY ONE: CMSP Sample Preparation Tools 2D HPLC Protein Separation System. Barocycler High Pressure Protein Lysis and Digestion (Pressure Biosciences, Inc). Isoelectric focussing apparatus (IPGphor (TM) (Amersham)). DIGE (Differential In-gel Electrophoresis) (Amersham).

9 DAY TWO: CMSP Mass Spectrometers St. Paul Campus Velos LTQ Orbitrap cap LC. Q-Exactive. LTQ cap LC Bruker Autoflex Speed MALDI-TOF/TOF GC GC TOF Pegasus 4D (LECO) LCQ Classic Minneapolis Campus Orbitrap Fusion (with Chen Lab) QTRAP Analytical and cap LC 4000 QTRAP Analytical and cap LC (with Parker Lab) Agilent Single-Quad LC-MS

10 DAY THREE: Computational Proteomics Software PEAKS (Bioinformatics Solutions) * Also available at Minnesota Supercomputing Institute ( ProteinPilot (Sciex)* Proteome Discoverer (Thermo) Scaffold (Proteome Software)* Galaxy-P platform (NSF grant)* (ProteinPilot, SearchGUI, Multiple software) MaxQuant (Max-Planck Institute)* Mascot (Matrix Science) Analyst QS (AB Sciex) and Xcalibur (Thermo) for raw data evaluation. ProteoWizard Progenesis (Waters)* Skyline (Washington University)

11 Workshop Goals Develop a fundamental understanding of Mass Spectrometry as applied to Proteomics Understand the requirements of biological sample preparation for introduction into Mass Spectrometers Understanding about input parameters and functioning of a Mass Spectrometer Understand Mass Spectrometry data output, interpretation and analysis. Ability to read and understand scientific literature on mass spectrometry. Instrumentation, facilities and resources available here at the University of Minnesota.

12 Workshop Itinerary August 9, 2016 Tuesday 9:05 10:55 Introduction to Proteomics I and II. (Gort 239) 15 minutes Break 11:10 12:00 Sample Preparation Basics. (Gort 239) Lunch Break 1:00 4:00 Workshops Ia, Ib and Ic (Gort 43) (split into groups)* August 10, 2016 Wednesday 9:00 9:45 Introduction to Mass Spectrometry (Gort 239) 9:50 11:05 Tandem MS and Intro to Liquid Chromatography-MS (Gort 239) 15 minutes Break 11:20 12:30 Specialized Applications in mass spectrometry (Gort 239) Lunch Break 1:30 4:00 Workshops IIa, IIb, IIc (Gort 43) (split into groups)* August 11, 2016 Thursday 9:00 9:40 ProteoInformatics Overview. (Gort 239) 10 minutes Break 9:50 10:45 Protein Identification. (Gort 239) 10:50 12:00 Protein Validation and Quantification. (Gort 239) Lunch Break 1:00 4:00 ProteoInformatics Workshop (Cargill Building 138 (computer lab))