HPLC 920-LC ANALY TIC AL LIQUID CHROMATOGR APHY SOLUTIONS.
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1 HPLC varian, inc. 92-LC ANALY TIC AL LIQUID CHROMATOGR APHY SOLUTIONS
2 VARIAN, INC. Integrating Performance and Reliability Fast HPLC The Varian 92-LC analytical high performance liquid chromatograph is a fully integrated, pre-configured and pre-tested system. Designed to get you quickly up and running, the automated Varian 92-LC is easy to customize, install and use. Whether you require excellent sensitivity, high sample throughput, sophisticated data handling, regulatory compliance, or special services, the Varian 92-LC offers a choice of configurations and options to meet all your analytical needs, today and into the future. The Varian 92-LC delivers: Fast LC Superb analytical performance Industry-leading detection limits Enhanced productivity Maximized uptime With Our Experience, Your HPLC Needs Are Covered The Varian 92-LC stems from a history of liquid chromatography excellence. For four decades, Varian s range of reliable and easy-to-use HPLC instruments has served pharmaceutical, environmental, chemical, and other industrial R&D and QA/QC laboratories worldwide. So when you buy a HPLC from Varian, you can rest assured you are investing in a relationship with proven experts in HPLC. The Varian 92-LC liquid chromatograph is a fully-integrated HPLC, delivering performance, reliability and flexibilty. Our customized HPLC systems, combined with the widest range of Varian-produced column chemistries, packing materials and dedicated software, provide versatile solutions for all your analytical to processscale separations. Varian is your one-stop-shop for everything HPLC! 2
3 Dedicated to Your Application Flexible Enough For All Your Applications Whether you need a robust HPLC for routine analysis, productivity for high-throughput applications, or flexibility and sensitivity to develop analytical methods in a regulated environment, the Varian 92-LC has many options to create the system that works best for you. Analyze thermally unstable compounds using the temperaturecontrolled autosampler. Confirm the presence of specific analytes, such as pesticides and explosive residues, using automatic column switching. Ensure retention time reproducibility using the precise column heater. Gain extra detectability for trace analytes such as polyaromatic hydrocarbons using the sensitive fluorescence detector. Add the refractive index (RI) or evaporative light scattering (ELS) detector for universal detection of carbohydrates or polymers. Isocratic pump Quaternary gradient pump Flexible Systems Routine Method Development Research Grade Degasser Autosampler Temperature-controlled autosampler Column heater Switching valve UV-Vis detector PDA detector Fluorescence detector RI detector ELS detector (external) Solvent organizer Storage compartment Standard feature Optional feature 3
4 VARIAN, INC. Designed For Performance Take Advantage of Fast LC We have extended the boundaries of HPLC performance with Fast LC, for faster method development, reduced solvent consumption, and increased sensitivity. The Varian 92-LC delivers: Rapid and reproducible gradient formations, a low carry-over autosampler and sensitive detectors all essential requirements for Fast LC. A low delay volume kit with semi-micro mixer, narrow bore plumbing and micro flow cell. New, short, sub 3 μm Varian columns packed with small particles, for even faster LC. mau A B C D RT (min) E F G Dual-piston pump accurately blends the mobile phase for isocratic runs, improving retention time reproducibility and eliminating the need for manually pre-mixed solvent blends. Built-in vacuum Degasser eliminates bubble formation, maintains pump prime, and improves pump reliability. Automated Sample Handling Good chromatography begins with careful sample introduction. With the Varian 92-LC: Use the built-in autosampler to run your instrument unattended, and increase your laboratory s productivity, without sacrificing data quality for throughput. Sample injection is synchronized with piston position in the solvent delivery cycle to improve retention time reproducibility for gradient runs. Sample-to-sample contamination is eliminated by the external needle wash and needle-in-flow path design, which flushes the needle and injection path with solvent between samples. Temperature-controlled sample compartment maintains sample integrity for thermally unstable compounds. Use up to two hundred 2 ml HPLC vials or three 96 format wellplates, to further boost productivity. Use 2.8 µm particle columns with rapid gradients for Fast LC, increasing productivity and sensitivity. Above, seven phenones were analyzed in less than 1.5 minutes. (A G: acetophenone, propiophenone, benzophenone, valerophenone, hexanophenone, heptanophenone, octanophenone) Column: Varian Ultra; Mobile phase: water/acetonitrile gradient, 45 to 1%; Detection: UV at 245 nm; Flow: 1.6 ml/min Superb Solvent Delivery The Varian 92-LC delivers virtually pulse-free solvent flow for outstanding performance. mau Sample Blank State-of-the-art dual-piston, pulseless, low pressure, quaternary gradient solvent delivery system improves detection limits by reducing noise at the detector. Precision proportioning valves maximize productivity and reproducibility by delivering rapid, accurate and reproducible gradients RT (min) Measure low concentration samples with ease flushing of the needle and injection path with solvent drastically reduces carry-over. Above, a caffeine sample injection immediately followed by a blank injection produced no discernable carry-over. Column: Varian Pursuit XRs C18; Mobile phase: 85% water, 15% acetonitrile; Detection: UV at 273 nm; Flow: 1 ml/min 4
5 Unrivalled Sensitivity and Precision Outstanding Detection Whether you require UV-Vis detection for traditional analyses or information rich detection using photodiode array (PDA) technology, Varian has a solution for you. You can also add a fluorescence detector for more sensitive and selective detection, and for non-uv-vis absorbing compounds you have the option of a refractive index or evaporative light scattering detector. The UV-Vis detector for the Varian 92-LC features: Deuterium and quartz-halogen lamps with high light throughput optics, ensuring low noise and excellent linearity across the wavelength range. Outstanding detection limits and good response across a wide concentration range. Wavelength time programming, allowing you to optimize the detector performance. The ability to ratio two wavelengths for monitoring peak purity during a run, giving you greater confidence in your results. The information-rich PDA detector for the Varian 92-LC features: Advanced double-beam optics design to minimize noise and drift, ensuring the best performance at the detection limits, and the highest analytical reproducibility. Automatic spectral searching against a library of known compounds, for fast identification of unknowns. Pre-aligned flow cells with focusing lenses to dramatically reduce refractive index effects, improving accuracy at low concentrations. Programmable slit widths from 1 μm to 16 μm for optimum light throughput, and unmatched performance and versatility. 124 diode PDA detector with a wide wavelength range from 19 nm up to 9 nm. mau Achieve excellent signal-to-noise ratios with the highly sensitive, low noise, high light throughput UV-Vis detector. The above anthracene example shows a signal-to-noise ratio of 36. Column: Varian Pursuit C8; Mobile phase: 85% acetonitrile, 15% water; Detection: UV at 251 nm; Flow:.3 ml/min Accurate Column Temperature Control A heated column compartment ensures rapid heating of the mobile phase for a consistent temperature profile along the column, providing retention time repeatability, and improved analytical reproducibility. The low delay volume pre-heater minimizes band broadening, maintaining chromatographic integrity. mau X Offset: Y Offset: A C Anthracene: 1pg on column RT (min) D 2 B RT (min) Excellent reproducibility is made possible by the precise column heater, providing consistent retention times and the utmost confidence in your results. Above shows 12 sample injections of ibuprofen oral suspension. (A D: polydatin, resveratrol, pterstilbene, emodin) Column: Varian Pursuit XRs C8; Mobile phase: 55% phosphoric acid, 45% acetonitrile; Detection: UV at 22 nm; Flow: 2 ml/min 5
6 VARIAN, INC. Galaxie Chromatography Software Simple. Flexible. Secure. Software That Drives Productivity With Varian Galaxie Chromatography Software, control one or multiple Varian LCs, GCs, or third party chromatographs from a single PC. With an updated look and simple controls, Galaxie is easier than ever to use. It features: Automated method development, optimization and validation using optional Fusion AE (Automated Experiment) for Galaxie. Significantly improve productivity in R&D and QC laboratories. System Suitability software automatically monitors the quality of chromatographic analysis, such as peak asymmetry and column efficiency. It determines the best action to take to correct potential problems and ensure the integrity of results. Instrument Maintenance Scheduling The Galaxie Instrument Maintenance window displays parameters such as pump strokes, number of injections and lamp intensity. As these parameters change, you can make informed decisions about when to perform maintenance on your instrument, for maximum uptime and peace of mind. You can also: Perform pre-defined instrument tests such as system noise, linearity, injection reproducibility, gradient accuracy, detector wavelength reproducibility and pressure integrity to ensure the highest confidence in instrument performance. Perform automated IQ/OQ tests to assist with operating in validated environments. Customized reports and powerful data handling capabilities, providing all the information you need in the format you want. User access control, audit trails and electronic signature capabilities for enhanced data security, assisting with operation in regulated environments, such as US FDA 21 CFR Part 11. An upgrade path to a Client/Server configuration to centralize system control and data handling. For an overview of the status of your Varian 92-LC, check the Instrument Status window. This dynamic, uptime enhancing window is an excellent diagnostic tool. The Instrument Maintenance window lets you know when seals, valves and filters will require changing, so you are always prepared. 6
7 Depend on Varian for All Your Chromatography Needs... From Microlitre to Litre Flows Varian s unique capabilities in separation technologies extend from sample clean-up using Bond Plexa, OMIX, Bond Elut and Chem Elut extraction products, through analysis/identification and scale-up of the separation, all the way to pilot plant. Analytical to Process Scale Separation Solutions from One Provider µl/min Our range of liquid chromatography systems provides microflow pumping on the Varian 32-MS LC/MS/MS systems, ml/min flow rates on the Varian 92-LC, Varian 94-LC analytical to preparative HPLC and SD-1 PrepStar, to the ultimate in seamless scale-up with Varian expertise in pilot plant separations at L/min flow rates on our SepTech skid HPLC systems. Varian Columns and Separation Media Whether you need capillaries, 5 mm preparative columns or multi-kilo batches of prep and process chromatography packings for columns up to 15 mm ID, Varian can help. Our unique and exciting chemistries include Pursuit Diphenyl and PFP, Polaris and high performance Pursuit XRs C18 and C8 columns. We manufacture every component of our proprietary phases, such as the world-famous Polymer Laboratories polymeric resins, and the pure silica gels in our Microsorb range. Our aim is to provide the highest quality columns, giving you better peak shapes, higher reproducibility and longer column lifetimes, all at an affordable price. We are committed to meeting your needs with a full range of consumable products and supplies. Call today to request your free copy of our comprehensive analytical supplies catalog. Or, shop online at Varian 32-MS Varian 94-LC Varian SD-1 PrepStar ml/min Bond Elut Plexa for SPE Pursuit XRs columns Load & Lock columns Varian SepTech L/min 7
8 VARIAN, INC. 92-LC ANALY TIC AL LIQUID CHROMATOGR APHY SOLUTIONS Varian, Inc. Supporting Customers Worldwide All products are eligible for on-demand support from a worldwide network of specialists who can assist with any need. Varian, Inc. offers: Comprehensive programs that include a full range of services from minor repairs, to complex applications development and training. Factory-qualified Field Support Representatives and Specialists, specifically chosen for each installation. Global support teams strategically located around the world. To learn more about Varian s industry-leading customer support, visit our Web site at Varian, Inc. Serving Industries Worldwide Biosciences Pharmaceuticals Clinical Research and Forensics Food and Agriculture Chemical Analysis Environmental Fuels and Energy Material Sciences Bond Elut, Chem Elut, Fusion AE, Galaxie, Load & Lock, Microsorb, OMIX, Plexa, Polaris, Polymer Laboratories, PrepStar, Pursuit, Varian and the Varian logo are trademarks or registered trademarks of Varian, Inc. in the U.S. and other countries. 27 Varian, Inc. Varian, Inc. North America: , Europe The Netherlands: Asia Pacific Australia: Latin America Brazil: Other sales offices and dealers throughout the world _ check our Web site. GC LC MS GPC/SEC AA ICP ICP-MS UV-Vis-NIR FT-IR Fluorescence Dissolution NMR MRI FTMS Consumables Data Systems SI /7 Printed in Australia
HPLC 940-LC ANALY TIC AL TO PREPARATIVE LIQUID CHROMATOGRAPHY SOLUTIONS.
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