WHAT HAPPENS AFTER A PHD?
|
|
|
- Kerry McGee
- 5 years ago
- Views:
Transcription
1 WHAT HAPPENS AFTER A PHD? MY BIOGRAPHICAL SKETCH Sandviks gård 29 August 2013 Pernilla Eliasson
2 Outline Postdoc in Utrecht Leaving academia and work at a Biotech company Working in the Pharma industry
3 Göteborg Astra Zeneca Göteborg BD Biosciences Västerås Linköping Master in Medical Biology Born in 1979 Biomedical Research School 2003 PhD in Cell Biology Utrecht, The Netherlands Posdoc
4 Linköping University Master in Medical Biology Biomedical research school PhD student Forum Scientium PhD student , Postdoc 3 months
5 December 11, 2009 LIVE AND LET DIE CRITICAL REGULATION OF SURVIVAL IN NORMAL AND MALIGNANT HEMATOPOIETIC STEM AND PROGENITOR CELLS Pernilla Eliasson Linköping University, Sweden Linköping University Medical Dissertations No Pre-Doc Doc Post-Doc Specialist
6 Pre-Doc Doc Post-Doc Specialist
7 Some Techniques used during my PhD Flow Cytometry, for analysis and cell sorting Microarray Real time PCR Western blot Confocal Microscopy Reconstitution in vivo assays Transfections and Transductions of viral vectors Gene silencing with sirna and shrna Pre-Doc Doc Post-Doc Specialist
8 FLT3- ITD PI3K Ras Tyrosine kinase inhibitor Raf Mek p Akt Erk p FoxO Survival p27 Bim Pre-Doc Doc Post-Doc Specialist
9 Flow Cytometry to measure apoptosis and cell cycle Western blot to measure phosphorylated proteins Transduction of mutated FLT3 receptor Gene silencing of the pro-apoptotic member Bim Pre-Doc Doc Post-Doc Specialist 9
10 FoxO and HSCs FoxO important for HSCs FoxO expressed in HSCs How can the same protein regulate both apoptosis and survival? DNA repair HSC Oxidative stress resistance FoxO Apoptosis Cell cycle arrest Pre-Doc Doc Post-Doc Specialist
11 Molecular Immunology Lab UMC Utrecht, The Netherlands M I L molecularimmunology.nl Paul Coffer Immunology -Regulatory T cells -Myeloid Malignancy -Hematopoietic stem cells Pre-Doc Doc Post-Doc Specialist 11
12 Postdoc FOXO transcription factors at the cross-roads of metabolism and autophagy Important regulators for Hematopoietic stem cells Pernilla Eliasson University Medical Center, Utrecht, The Netherlands Pre-Doc Doc Post-Doc Specialist 12
13 Where? Postdoc Lab more important than subject Sweden, Europe or USA/Canada When and how to start the application process? In the mind from first day of PhD Talk to people at home and at conferences, write s How long? From 6 months to 4 years, depending on chose later in life Finansing- Salary or scolorship? Time Salary from host institute, after 1 year Postdoctoral fellowship, from The Swedish Research Council (VR) Social security 6-12 moths A-kassa Pension (Easy within EU)
14 Postdoc Advantages Experience (for life, private and no matter what career way you later choose) New Friends, new culture, new language etc Fun! Extended youth New challenges, international top research lab Disadvantages Family, Friends Economy (not in my case)
15 Postdoc
16 Flow Cytometry to measure apoptosis, autophagy and phenotypic markers Western blot, mainly phosphorylated proteins RNA silencing of both primary blood cells and cell lines Confocal Microspopy for co-localisation of autophagosomes and lysosomes Pre-Doc Doc Post-Doc Specialist 17
17 Postdoc Salary extended 6 months Applied for grants At the same time: Applied for jobs in Sweden
18 Some Techniques used during my PhD and Postdoc Flow Cytometry, for analysis and cell sorting Microarray Real time PCR Western blot Confocal Microscopy Reconstitution in vivo assays Transfections and Transductions of viral vectors Gene silencing with sirna and shrna Pre-Doc Doc Post-Doc Specialist
19 Application Specialist in Flow Cytometry FACS analysis training FACS sorting training and seminars Multicolor workshop Phosflow workshop Application support Pre-Doc Doc Post-Doc Specialist 20
20 Application Specialist at BD Bioscience Pre-Doc Doc Post-Doc Specialist
21 Application Specialist at BD Bioscience
22 Application Specialist at BD Bioscience Advantages Experience from other environment Traveling, hotels, dinners Meet new people Fun! Learn other people something they need in there daily job Being an expert is an ego boost Disadvantages Seldom follow up and able to follow progression in projects Hard to have a normal life after working hours Every week new city, new lab and new people Lonely No research! Pre-Doc Doc Post-Doc Specialist
23 Senior Researcher at AstraZeneca Started as a consultant for Proffice Needed a FACS expert Involved in many different projects in research related to type 2 diabetes and diabetic nephropathy Developing new functional cell-based assays FACS support in several projects Finding new potential targets Pre-Doc Doc Post-Doc Specialist Researcher
24 Senior Researcher at AstraZeneca Advantages Doing what you do the best (helping each other) Working together with other people in research projects (more research than I expected!) Responsibility and freedom Goal oriented with dead lines to reach next mile stone Being the expert but also learn new research areas Colleagues and nice physical training possibilities at local gym Disadvantages Not as much social activities with colleagues after work Less control and overview over projects than in academia Pre-Doc Doc Post-Doc Specialist Researcher
25 Partying with BD people Beer drinking with Postdoc colleagues in Utrecht
Position Description November 2018
Position Description November 2018 MISSION The mission of St Vincent s Institute (SVI) is to carry out the highest quality laboratory based biomedical research, in order to make discoveries that will improve
Supplementary Materials for
www.sciencesignaling.org/cgi/content/full/5/233/ra50/dc1 Supplementary Materials for Epidermal Growth Factor Receptor Is Essential for Toll-Like Receptor 3 Signaling Michifumi Yamashita, Saurabh Chattopadhyay,
SureSilencing sirna Array Technology Overview
SureSilencing sirna Array Technology Overview Pathway-Focused sirna-based RNA Interference Topics to be Covered Who is SuperArray? Brief Introduction to RNA Interference Challenges Facing RNA Interference
Dr Amy Prosser Careers in Research: Industry
www.rsb.org.uk Dr Amy Prosser Careers in Research: Industry @RoyalSocBio #BiologyWeek #RSBcareersDay Careers in Research: Working in the Pharma Industry Dr Amy Prosser My Career History 2011 Completed
7.06 Problem Set #3, 2006
7.06 Problem Set #3, 2006 1. You are studying the EGF/Ras/MAPK pathway in cultured cells. When the pathway is activated, cells are signaled to proliferate. You generate various mutants described below.
Flow cytometry Stained cells were analyzed and sorted by SORP FACS Aria (BD Biosciences).
Mice C57BL/6-Ly5.1 or -Ly5.2 congenic mice were used for LSK transduction and competitive repopulation assays. Animal care was in accordance with the guidelines of Keio University for animal and recombinant
Post-doctoral Research Fellow
Candidate Brief Post-doctoral Research Fellow Reference: R180142 Salary: Grade 8, 32,548 to 38,833 per annum Contract Type: Fixed Term (until 1st May 2020) Basis: Full Time Closing Date: 23.59 hours BST
OmicsLink shrna Clones guaranteed knockdown even in difficult-to-transfect cells
OmicsLink shrna Clones guaranteed knockdown even in difficult-to-transfect cells OmicsLink shrna clone collections consist of lentiviral, and other mammalian expression vector based small hairpin RNA (shrna)
Molecular mechanisms and epigenetic regulation of adenovirus genome structure in persistent infection
MSc MOL BIOTECH 12 003 Molecular mechanisms and epigenetic regulation of adenovirus genome structure in persistent infection Sibel Ciftci Degree project in molecular biotechnology, 2012 Examensarbete i
CURRICULUM VITAE. Educational Qualification: DEGREE INSTITUTION YEAR PROGRESS. Bharathidasan University Tamil Nadu
CURRICULUM VITAE M.BHARATH VIJAYARAGAVAN Masters in Biomedical sciences Nationality: Indian E-mail: bharathragavan@gmail.com Mobile: 91-9911738539 Sex: Male; Age: 23 Objective: Cell Signaling is an important
Supplementary Information
Supplementary Information Supplementary Figures Supplementary Figure 1. MLK1-4 phosphorylate MEK in the presence of RAF inhibitors. (a) H157 cells were transiently transfected with Flag- or HA-tagged MLK1-4
sherwood - UltramiR shrna Collections
sherwood - UltramiR shrna Collections Incorporating advances in shrna design and processing for superior potency and specificity sherwood - UltramiR shrna Collections Enabling Discovery Across the Genome
Boston Kobayashi Lab Ayako Hirata
Boston Kobayashi Lab Ayako Hirata Kyoto University Faculty of Medicine 4 th year medical student This year, I did my internship at Beth Israel Deaconess Medical Center in Boston, Massachusettes from July
TE5 KYSE510 TE7 KYSE70 KYSE140
TE5 KYSE5 TT KYSE7 KYSE4 Supplementary Figure. Hockey stick plots showing input normalized, rank ordered H3K7ac signals for the candidate SE-associated lncrnas in this study. Rpm Rpm Rpm Chip-seq H3K7ac
Supplemental Information Inventory
Cell Stem Cell, Volume 6 Supplemental Information Distinct Hematopoietic Stem Cell Subtypes Are Differentially Regulated by TGF-β1 Grant A. Challen, Nathan C. Boles, Stuart M. Chambers, and Margaret A.
PROTEOMICS AND FUNCTIONAL GENOMICS PRESENTATION KIMBERLY DONG
PROTEOMICS AND FUNCTIONAL GENOMICS PRESENTATION KIMBERLY DONG A Lentiviral RNAi Library for Human and Mouse Genes Applied to an Arrayed Viral High-Content Screen Jason Moffat,1,2,4,10 Dorre A. Grueneberg,1,10
Figure S1 (related to Fig. 1): The prototypical mitochondrial pathway of apoptosis is involved in cell-death of v-src-transformed cells.
Figure S1 (related to Fig. 1): The prototypical mitochondrial pathway of apoptosis is involved in cell-death of v-src-transformed cells. (A) Non-transformed (Control cells) and v-srctransformed 3T3 cells
11/19/2013. Janine Zankl FACS Core Facility 13. November Cellular Parameters. Cellular Parameters. Monocytes. Granulocytes.
DEPARTEMENT BIOZENTRUM Janine Zankl FACS Core Facility 13. November 2013 Cellular Parameters Granulocytes Monocytes Basophils Neutrophils Lymphocytes Eosinophils Cellular Parameters 1 What Is Flow Cytometry?
LoxP. SFFV mcherry wpre SIN-LTR. shrna-p53. LoxP. shrna-scr
LeGO-C-p53-shRNA SIN-LTR? RRE cppt H1/TO shrna-p53 SFFV mcherry wpre SIN-LTR LeGO-C-scr-shRNA SIN-LTR? RRE cppt H1/TO shrna-scr SFFV mcherry wpre SIN-LTR Supplementary Figure 3A: Scheme of the lentiviral
BIOL 461/ 661 Cell Biology 4 Credits Instructor: Dr. Kristin O Brien. T/TH 11:30-1:00, Irving I 208 Office hours: M 9-10, TH 3-4
BIOL 461/ 661 Cell Biology 4 Credits Instructor: Dr. Kristin O Brien Prerequisites: BIOL 362 Principals of Genetics Office: 226 Arctic Health CHEM 321 Organic Chemistry Laboratory: 229 Arctic Health T/TH
Time allowed: 2 hours Answer ALL questions in Section A, ALL PARTS of the question in Section B and ONE question from Section C.
UNIVERSITY OF EAST ANGLIA School of Biological Sciences Main Series UG Examination 2017-18 CELL BIOLOGY BIO-5005B Time allowed: 2 hours Answer ALL questions in Section A, ALL PARTS of the question in Section
SUPPLEMENTAL MATERIAL. Supplemental Methods. Cumate solution was from System Biosciences. Human complement C1q and complement
SUPPLEMENTAL MATERIAL Supplemental Methods Reagents Cumate solution was from System Biosciences. Human complement Cq and complement C-esterase inhibitor (C-INH) were from Calbiochem. C-INH (Berinert) for
Hector R. Wong, MD Division of Critical Care Medicine Cincinnati Children s Hospital Medical Center Cincinnati Children s Research Foundation
Mechanisms of Sepsis Exploiting Pathogenic Genes Hector R. Wong, MD Division of Critical Care Medicine Cincinnati Children s Hospital Medical Center Cincinnati Children s Research Foundation Critical Care
Supporting Information
Supporting Information SI Materials and Methods RT-qPCR The 25 µl qrt-pcr reaction mixture included 1 µl of cdna or DNA, 12.5 µl of 2X SYBER Green Master Mix (Applied Biosystems ), 5 µm of primers and
Translational Research
Translational Research Paddy Johnston Chair, MRC Translational Research Overview Group October 2012 Translational Research Strategy and its delivery To accelerate patient benefit and increase economic
TOOLS sirna and mirna. User guide
TOOLS sirna and mirna User guide Introduction RNA interference (RNAi) is a powerful tool for suppression gene expression by causing the destruction of specific mrna molecules. Small Interfering RNAs (sirnas)
Heparan Sulphate in Breast Cancer Low, Y.L.A 1 and Yip, C.W.G 2
Heparan Sulphate in Breast Cancer Low, Y.L.A 1 and Yip, C.W.G 2 Department of Anatomy, Yong Loo Lin School of Medicine, National University of Singapore MD10, 4 Medical Drive, Singapore 117597 ABSTRACT
Supplementary Figure 1. Expressions of stem cell markers decreased in TRCs on 2D plastic. TRCs were cultured on plastic for 1, 3, 5, or 7 days,
Supplementary Figure 1. Expressions of stem cell markers decreased in TRCs on 2D plastic. TRCs were cultured on plastic for 1, 3, 5, or 7 days, respectively, and their mrnas were quantified by real time
Supplementary Figure 1. IFN-γ induces TRC dormancy. a, IFN-γ induced dormancy
Supplementary Figure 1. IFN-γ induces TRC dormancy. a, IFN-γ induced dormancy of various tumor type TRCs, including H22 (murine hepatocarcinoma) and CT26 (murine colon cancer). Bar, 50 µm. b, B16 cells
ncounter Vantage 3D RNA:Protein Solid Tumor Assay
PRODUCT BULLETIN Product Highlights RNA More data from your precious samples; RNA:Protein profiling requires just 1 μg of protein or FFPE slides (protein-only analysis requires ng or 1 FFPE slide) Simple
ijobs Career Panel: Tech and Sales Support Tuesday October 3, :30-6pm 675 Hoes Lane West Room V10 Piscataway, NJ
ijobs Career Panel: Tech and Sales Support Tuesday October 3, 2017 4:30-6pm 675 Hoes Lane West Room V10 Piscataway, NJ Advice for those interested in careers in tech and sales support Types of jobs in
Tumor Growth Suppression Through the Activation of p21, a Cyclin-Dependent Kinase Inhibitor
Tumor Growth Suppression Through the Activation of p21, a Cyclin-Dependent Kinase Inhibitor Nicholas Love 11/28/01 A. What is p21? Introduction - p21 is a gene found on chromosome 6 at 6p21.2 - this gene
Supplementary Figure 1, related to Figure 1. GAS5 is highly expressed in the cytoplasm of hescs, and positively correlates with pluripotency.
Supplementary Figure 1, related to Figure 1. GAS5 is highly expressed in the cytoplasm of hescs, and positively correlates with pluripotency. (a) Transfection of different concentration of GAS5-overexpressing
From Bench To Clinic : a personal journey Career Research Advancement Focused Training
From Bench To Clinic : a personal journey Career Research Advancement Focused Training February 13, 2018 Irina Tcherepanova Ph.D. Vice President of Translational Medicine Argos Therapeutics Inc. FDA view
ER stress and autophagy: new players in the mechanism of action and drug resistance of SUPPLEMENTAL DATA
ER stress and autophagy: new players in the mechanism of action and drug resistance of the cyclin-dependent kinase inhibitor SUPPLEMENTAL DATA METHODS Confocal immunofluorescence microscopy of fixed cells:
Measuring gene expression and protein production
Measuring gene expression and protein production Module 3, Lecture 5! 20.109 Spring 2013! Lessons from Lecture 4 Always look for the recovery file! The myth of multitasking! task-switching taps processing
Job Description Postdoctoral Research Scientist (PRG-PD-BB) Length of appointment Three years in the first instance
Job Description Postdoctoral Research Scientist (PRG-PD-BB) Department Epigenetics Grade BI 6 Salary range 30,751 to 34,166 per annum Length of appointment Three years in the first instance Location Babraham
Revision Checklist for Science Signaling Research Manuscripts: Data Requirements and Style Guidelines
Revision Checklist for Science Signaling Research Manuscripts: Data Requirements and Style Guidelines Further information can be found at: http://stke.sciencemag.org/sites/default/files/researcharticlerevmsinstructions_0.pdf.
Job Description Postdoctoral Research Scientist
Job Description Postdoctoral Research Scientist Department Epigenetics Grade BI 6 Salary range 30,751 to 34,166 per annum Length of appointment Up to 18 months Location Babraham Institute Working Hours
Concepts and Methods in Developmental Biology
Biology 4361 Developmental Biology Concepts and Methods in Developmental Biology June 16, 2009 Conceptual and Methodological Tools Concepts Genomic equivalence Differential gene expression Differentiation/de-differentiation
Old EXAM 1 BIO409/509 NAME. Please number your answers and write them on the attached, lined paper.
Old EXAM 1 BIO409/509 NAME Please number your answers and write them on the attached, lined paper. 1) Describe euchromatin and heterochromatin. Which form of chromatin would the insulin gene be found in
The Institute of Microbiology of the Czech Academy of Sciences is looking for. four POSTDOCTORAL FELLOW positions in the fields of:
4 Postdoctoral Fellow positions in microbiology, molecular biology and ecology The Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic The Institute of Microbiology of the
SULTAN QABOOS UNIVERSITY COURSE OUTLINE PROGRAM: Biotechnology. Field or Work Placement
SULTAN QABOOS UNIVERSITY COURSE OUTLINE PROGRAM: Biotechnology 1. Course Code BIOL4500 2. Course Title Cell Biology 3. Credits 3 4. Pre-requisite Course(s) BIOL 2101 5. Co-requisite Course(s) 6. Equivalent
Department of Pharmacology and Toxicology. School of Medicine Medical Sciences Campus University of Puerto Rico
Department of Pharmacology and Toxicology School of Medicine Medical Sciences Campus University of Puerto Rico What is Pharmacology? Pharmacology is the science that studies how drugs, toxic substances
OriGene GFC-Arrays for High-throughput Overexpression Screening of Human Gene Phenotypes
OriGene GFC-Arrays for High-throughput Overexpression Screening of Human Gene Phenotypes High-throughput Gene Function Validation Tool Introduction sirna screening libraries enable scientists to identify
THE UNIVERSITY OF MANCHESTER PARTICULARS OF APPOINTMENT FACULTY OF MEDICAL AND HUMAN SCIENCES INSTITUTE OF CANCER SCIENCES
THE UNIVERSITY OF MANCHESTER PARTICULARS OF APPOINTMENT FACULTY OF MEDICAL AND HUMAN SCIENCES INSTITUTE OF CANCER SCIENCES SCIENTIFIC OFFICER WITH MICHELA GAROFALO TRANSCRIPTIONAL NETWORKS IN LUNG CANCER
Bioassays are required for a
B I O P R O C E S S TECHNICAL Bioassay Survey 006 007 Cell-Based Bioassays in the Biopharmaceutical Industry C. Jane Robinson, Laureen E. Little, and Hans-Joachim Wallny Bioassays are required for a variety
Stabilization of the Transcription Factor Foxp3 by the Deubiquitinase USP7 Increases Treg-Cell-Suppressive Capacity
Immunity, Volume 39 Supplemental Information Stabilization of the Transcription Factor Foxp3 by the Deubiquitinase USP7 Increases Treg-Cell-Suppressive Capacity Jorg van Loosdregt, Veerle Fleskens, Juan
Supplementary information
Supplementary information Supplementary Figure 1 (a) EM image of the pyramidal layer of wt mice. CA3 pyramidal neurons were selected according to their typical alignment, size and shape for subsequent
Transcription Factor Runx3 Is Induced by Influenza A Virus and Double-Strand RNA and
Transcription Factor Is Induced by Influenza A Virus and Double-Strand RNA and Mediates Airway Epithelial Cell Apoptosis Huachen Gan 1, Qin Hao 1, Steven Idell 1,2 & Hua Tang 1 1 Department of Cellular
A New Cellular and Molecular Engineering Curriculum at Rice University
Session A New Cellular and Molecular Engineering Curriculum at Rice University Ka-Yiu San, Larry V. McIntire, Ann Saterbak Department of Bioengineering, Rice University Houston, Texas 77005 Abstract The
Dual PI3K/ERK inhibition induces necroptotic cell death of Hodgkin Lymphoma cells through IER3 downregulation
Dual PI3K/ERK inhibition induces necroptotic cell death of Hodgkin Lymphoma cells through IER3 downregulation *Silvia Laura Locatelli, 1 Giuseppa Careddu, 1 Giuliano Giuseppe Stirparo, 1 Luca Castagna,
Effects of metformin on Sirt1, Nrf2 and AhR expression in cancer cells
A Dissertation for the Degree of Doctor of Philosophy Effects of metformin on Sirt1, Nrf2 and AhR expression in cancer cells Department of Pharmacy Graduate School Chungnam National University By Minh
PCR Arrays. An Advanced Real-time PCR Technology to Empower Your Pathway Analysis
PCR Arrays An Advanced Real-time PCR Technology to Empower Your Pathway Analysis 1 Table of Contents 1. Introduction to the PCR Arrays 2. How PCR Arrays Work 3. Performance Data from PCR Arrays 4. Research
Supplementary Figure 1: Overexpression of EBV-encoded proteins Western blot analysis of the expression levels of EBV-encoded latency III proteins in
Supplementary Figure 1: Overexpression of EBV-encoded proteins Western blot analysis of the expression levels of EBV-encoded latency III proteins in BL2 cells. The Ponceau S staining of the membranes or
Opportunities for Accelerating Cell Line Development and Beyond
Opportunities for Accelerating Cell Line Development and Beyond European CM&C Strategy Forum May 24, 2017 Christopher Frye, Ph.D. Research Advisor & Group Leader Bioprocess R&D Presentation Outline CM&C
Gene therapy. Findings by Alert
Gene therapy Published Mar 21, 2000 Version 1 Findings by Alert Research in gene therapy has increased dramatically during the past 15 years, particularly in the United States. The research has encompassed
Li et al., Supplemental Figures
Li et al., Supplemental Figures Fig. S1. Suppressing TGM2 expression with TGM2 sirnas inhibits migration and invasion in A549-TR cells. A, A549-TR cells transfected with negative control sirna (NC sirna)
3'UTR elements inhibit Ras-induced C/EBPß posttranslational activation and senescence in tumor cells
Manuscript EMBO-2011-77536 3'UTR elements inhibit Ras-induced C/EBPß posttranslational activation and senescence in tumor cells Sandip K. Basu, Radek Malik, Christopher J. Huggins, Sook Lee, Thomas Sebastian,
Recent years have witnessed an expansion in the disciplines encompassing drug
Course overview Recent years have witnessed an expansion in the disciplines encompassing drug discovery outside the pharmaceutical industry. This is most notable with a significant number of Universities
Supplementary Material. TRIB3 inhibits proliferation and promotes osteogenesis in hbmscs by regulating the. ERK1/2 signaling pathway
Supplementary Material TRIB3 inhibits proliferation and promotes osteogenesis in hbmscs by regulating the ERK1/2 signaling pathway Cui Zhang 1, Fan-Fan Hong 1, Cui-Cui Wang 1, Liang Li 1, Jian-Ling Chen
Curriculum Vitae. Mahina Tabassum Mitul. Contact Number:
Curriculum Vitae Mahina Tabassum Mitul Contact Number: 01779010515 E-mail: mahina18@gmail.com Academic Qualification: Degree University/ Board Major/Concentration Passing Year MS University of Dhaka Biochemistry
Course Agenda. Day One
Course Agenda BioImmersion: Biotech for the Non-Scientist A three-day, in-depth course that provides the background required for understanding today s fast-paced biotech marketplace. Beginning with an
CENTER FOR BIOTECHNOLOGY
CENTER FOR BIOTECHNOLOGY Keith A. McGee, Ph.D., Program Director Math and Science Building, 3 rd Floor 1000 ASU Drive #870 Phone: 601-877-6198 FAX: 601-877-2328 Degree Offered Required Admission Test M.
Information Extraction from Biomedical Text
Information Extraction from Biomedical Text BMI/CS 776 www.biostat.wisc.edu/bmi776/ Mark Craven craven@biostat.wisc.edu Spring 2009 The Information Extraction Task: Named Entity Recognition Analysis of
Supplementary Fig S1 Nutlin-3a treatment does not affect cell cycle progression in the absence
Supplementary Figure Legends Supplementary Fig S1 Nutlin-3a treatment does not affect cell cycle progression in the absence of p53 or p21. HCT116 cells which were null for either p53 (A) or p21 (B) were
Investigating the regulation of mirna biogenesis and Argonaute2 by RNA binding proteins
Investigating the regulation of mirna biogenesis and Argonaute2 by RNA binding proteins Patrick Peter Connerty Supervisor: Gyorgy Hutvagner Thesis Submitted for the Degree of Doctor of Philosophy (Science)
Thermo Scientific Dharmacon SMARTvector 2.0 Lentiviral shrna Particles
Thermo Scientific Dharmacon SMARTvector 2.0 Lentiviral shrna Particles Long-term gene silencing shrna-specific design algorithm High titer, purified particles Thermo Scientific Dharmacon SMARTvector shrna
DEPARTMENT OF BIOCHEMISTRY AND MOLECULAR BIOLOGY
Department of Biochemistry and Molecular Biology 1 DEPARTMENT OF BIOCHEMISTRY AND MOLECULAR BIOLOGY Office in Molecular and Radiological Biosciences Building, Room 111 (970) 491-5602 bmb.colostate.edu
Pre-made Reporter Lentivirus for p53 Signal Pathway
Pre-made Reporter for p53 Signal Pathway Cat# Product Name Amounts LVP977-P or: LVP977-P-PBS P53-GFP (Puro) LVP978-P or: LVP978-P-PBS P53-RFP (Puro) LVP979-P or: LVP979-P-PBS P53-Luc (Puro) LVP980-P or:
Page 1. AMPTask Force on MLS Molecular Pathology Curriculum Dear Molecular Diagnostics Laboratory Manager,
AMPTask Force on MLS Molecular Pathology Curriculum Dear Molecular Diagnostics Laboratory Manager, Recently the Association for Molecular Pathology (AMP) has decided to develop a suggested curriculum in
Supplementary Figure S1
Supplementary Figure S1 Supplementary Figure S1. CD11b expression on different B cell subsets in anti-snrnp Ig Tg mice. (a) Highly purified follicular (FO) and marginal zone (MZ) B cells were sorted from
Loss of Cul3 in Primary Fibroblasts
PSU McNair Scholars Online Journal Volume 5 Issue 1 Humans Being: People, Places, Perspectives and Processes Article 15 2011 Loss of Cul3 in Primary Fibroblasts Paula Hanna Portland State University Let
Supplemental Table S1. RT-PCR primers used in this study
Supplemental Table S1. RT-PCR primers used in this study -----------------------------------------------------------------------------------------------------------------------------------------------
ERASMUS STUDENT MOBILITY PLACEMENT FINAL REPORT
ERASMUS STUDENT MOBILITY PLACEMENT FINAL REPORT Motivation to participate in Erasmus programe My interest in Biology and science is extremly strong and a great part of my life. I have been studying Biology
Research Advances in the Development of Transgenic and Gene Edited Products in Sri Lanka
Research Advances in the Development of Transgenic and Gene Edited Products in Sri Lanka Dr. Pradeepa C.G. Bandaranayake Director, Agricultural Biotechnology Centre Faculty of Agriculture University of
Figure S1. Mechanism of ON induced MM cell death.
Figure S1. Mechanism of ON123300 induced MM cell death. To elucidate whether ON123300 induced decrease of viability of MM cells was associated with induction of apoptosis, the number of apoptotic cells
(Length: 16 min; file size: 9.4 MB; file format: mp3; location:
Science Signaling Podcast: 25 October 2011 Adam A. Friedman 1, 2, 3, Norbert Perrimon 1, 2, and Annalisa M. VanHook 4 1. 1Department of Genetics, Harvard Medical School, 77 Avenue Louis Pasteur, Boston,
Building a Career in the Pharmaceutical Industry: Personal Reflections. Carlo Nalin, PhD Executive Director Novartis Oncology
Building a Career in the Pharmaceutical Industry: Personal Reflections Carlo Nalin, PhD Executive Director Novartis Oncology 2 Career Development: Personal Reflections C Nalin 5 April 2016 Cornell BEST
Supplementary Information
Supplementary Information Supplementary Figure S1 (a) P-cRAF colocalizes with LC3 puncta. Immunofluorescence (IF) depicting colocalization of P-cRAF (green) and LC3 puncta (red) in NIH/3T3 cells treated
Confocal immunofluorescence microscopy
Confocal immunofluorescence microscopy HL-6 and cells were cultured and cytospun onto glass slides. The cells were double immunofluorescence stained for Mt NPM1 and fibrillarin (nucleolar marker). Briefly,
In-Cell Western QUANTITATIVE CELL-BASED ASSAYS ACCURATE QUANTIFICATION MULTIPLEX DETECTION HIGH SENSITIVITY DIRECT DETECTION
In-Cell Western QUANTITATIVE CELL-BASED ASSAYS ACCURATE QUANTIFICATION MULTIPLEX DETECTION HIGH SENSITIVITY DIRECT DETECTION IMMUNOFLUORESCENT-BASED ASSAY MULTIPLE TARGETS In-Cell Western QUANTITATIVE
Network System Inference
Network System Inference Francis J. Doyle III University of California, Santa Barbara Douglas Lauffenburger Massachusetts Institute of Technology WTEC Systems Biology Final Workshop March 11, 2005 What
Supplementary Materials for
www.sciencesignaling.org/cgi/content/full/6/282/ra53/dc1 Supplementary Materials for Estrogen Alters the Splicing of Type 1 Corticotropin-Releasing Hormone Receptor in Breast Cancer Cells Suchita Lal,
ASMS Workshop Report
ASMS Workshop Report 1.Title of workshop and those presiding Title: Get Ready to Become an MS Rising Star -Young Mass Spectrometrists Workshop Presided by Olga Friese (Pfizer) and Dian Su (Genentech) 2.Date
General Education Learning Outcomes
BOROUGH OF MANHATTAN COMMUNITY COLLEGE City University of New York Department of Science Title of Course: Cell Biology Class hours 3 BIO Section: 260 Lab hours 3 Semester Spring 2018 Credits 4 Schedule:
7.06 Problem Set #3, Spring 2005
7.06 Problem Set #3, Spring 2005 1. The Drosophila compound eye is composed of about 800 units called ommatidia. Each ommatidium contains eight photoreceptor neurons (R1 through R8), which develop in a
b alternative classical none
Supplementary Figure. 1: Related to Figure.1 a d e b alternative classical none NIK P-IkBa Total IkBa Tubulin P52 (Lighter) P52 (Darker) RelB (Lighter) RelB (Darker) HDAC1 Control-Sh RelB-Sh NF-kB2-Sh
Single-Cell Westerns. 1,000 cells at a time.
Single-Cell Westerns. 1,000 cells at a time. Meet Milo. He does Single-Cell Westerns that ll let you measure protein expression in thousands of single cells in a single run. You ll get it all done in 4
SUPPLEMENTAL FIGURE LEGENDS
SUPPLEMENTAL FIGURE LEGENDS Suppl. Fig. 1. Notch and myostatin expression is unaffected in the absence of p65 during postnatal development. A & B. Myostatin and Notch-1 expression levels were determined
Cellometer Vision CBA
Features of the Vision CBA Image Cytometry System All-in-One System Basic cell counting, primary cell viability, and cellbased assays. See for Yourself Why the Top Ten Pharmaceutical Companies Trust Cellometer
A Survey of Genetic Methods
IBS 8102 Cell, Molecular, and Developmental Biology A Survey of Genetic Methods January 24, 2008 DNA RNA Hybridization ** * radioactive probe reverse transcriptase polymerase chain reaction RT PCR DNA
Conference Feedback Report
Conference Feedback Report Earl Prinsloo, Ph.D South African Society Biochemistry and Molecular Biology 2010 SASBMB2010 18-20 January 2010 Illanga Estate Center Bloemfontein, South Africa Biomedical Biotechnology
The Pathways to Understanding Diseases
FOR PHARMA & LIFE SCIENCES White paper The Pathways to Understanding Diseases Deciphering complex biological processes Executive Summary Understanding complex biological processes is critical whether doing
GENDER EQUALITY PLAN RESEARCH CENTRE
GENDER EQUALITY PLAN RESEARCH CENTRE 2016-2018 ABOUT The Gender Equality Plan (GEP) for the Research Centre of Institut Curie was conceived in the framework of the H2020 project LIBRA. LIBRA is an ambitious
BIOCHEM + MOLE BIOLOGY- BC (BC)
Biochem + Mole Biology-BC (BC) 1 BIOCHEM + MOLE BIOLOGY- BC (BC) Courses BC 192 Biochemistry Freshman Seminar Credits: 2 (1-0-1) Introduction to curriculum and career options for biochemistry majors. Registration
Establishing sirna assays in primary human peripheral blood lymphocytes
page 1 of 7 Establishing sirna assays in primary human peripheral blood lymphocytes This protocol is designed to establish sirna assays in primary human peripheral blood lymphocytes using the Nucleofector
Regulation of metabolic pathways
Regulation of metabolic pathways Bacterial control of gene expression Operon: cluster of related genes with on/off switch Three Parts: 1. Promoter where RNA polymerase attaches 2. Operator on/off, controls
Supplementary information; Mungamuri et al., 2006
Supplementary information; Mungamuri et al., 6 Antibodies used for western blotting: The following antibodies were used for western blotting: antiser473 Akt (#4), antiakt (#97), antiser9 Gsk 3b (#9336),
Development of myostatin inhibitory peptides for muscle atrophic disorders
Cédric RENTIER, Ph.D. Tokyo University of Pharmacy and Life Sciences Department of Medicinal Chemistry Development of myostatin inhibitory peptides for muscle atrophic disorders European Research Day 2016