Andrew D. Hollenbach, PhD Louisiana State University Health Sciences Center Department of Genetics 533 Bolivar St. New Orleans, LA 70112

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1 Andrew D. Hollenbach, PhD Louisiana State University Health Sciences Center Department of Genetics 533 Bolivar St. New Orleans, LA Phone: (504) EDUCATION Research Associate (2001-present) Postdoctoral Fellow ( ) Focus: The identification of proteins that interact with the transcription factor Pax3, the regulation of Pax3 transcriptional activity through protein-protein interactions, the alteration of normal Pax3 transcriptional activity by the oncogenic fusion protein Pax3- FKHR, and the development of a viable cellular myoblast model to study the biological effects of the Pax3-FKHR oncogenic fusion protein on the generation of the solid muscle tumor alveolar rhabdomyosarcoma. The Johns Hopkins University, Baltimore, Maryland Ph.D., Biochemistry ( ) Dissertation: Studies on Periplasmic Binding Protein-Dependent Thiamin Transport in Escherichia coli. The University of Delaware, Newark, Delaware Bachelor of Science (Cum Laude), Chemistry (1989) EXPERIENCE 2003-present Assistant Professor Louisiana State University Health Sciences Center, Department of Genetics, New Orleans, Louisiana Research Associate Postdoctoral Fellow

2 Graduate Researcher The Johns Hopkins University, Baltimore, Maryland Undergraduate Researcher The University of Delaware, Newark, Delaware TEACHING EXPERIENCE Undergraduate Teaching Assistant Freshman Chemistry (September 1988-December 1988) Freshman Chemistry (Winter term 1989) Freshman Biochemistry (January 1989-May 1989) AWARDS and HONORS The Harry D. Kruse Award in Nutrition and Public Health The Johns Hopkins University, 1993 Phi Lambda Upsilon, Honorary Chemical Society The University of Delaware, Science and Engineering Research Scholarship The University of Delaware, Delta Phi Alpha, Honorary German Language Society The University of Delaware, ADMINSTRATIVE ORGANIZATIONS Member St. Jude Children s Research Hospital Postdoctoral Review Committee Member Executive Council for the St. Jude Children s Research Hospital Council of Postdoctoral Fellows 2001-present Member St. Jude Children s Research Hospital Institutional Animal Care and Usage Committee

3 PUBLICATIONS Washabaugh, M. W., Yang, C. C., Hollenbach, A. D. and Chen, P. (1993) Hydrolysis of thiamin: evidence for rate limiting breakdown of the tricyclic dihydrothiachromine intermediate in neutral aqueous solution. Bioorg. Chem. 21, Hollenbach, A. D.*, Dickson, K. and Washabaugh, M. W. (2002) Overexpression, purification, and characterization of the periplasmic space thiamin-binding protein of the thiamin traffic ATPase in Escherichia coli. Prot. Exp. Purif. 25, Hollenbach, A. D. *, Dickson, K. and Washabaugh, M. W. (2002) Thiamin transport in Escherichia coli: the mechanism of inhibition by the sulfhydrylspecific modifier N-ethylmaleimide. Biochim. Biophys. Acta. 1564, Lam, P. Y.-P., Sublett, J. E., Hollenbach, A. D. and Roussel, M. F. (1999) The oncogenic potential of the Pax3-FKHR fusion protein requires the Pax3 homeodomain recognition helix but not the Pax3 paired-box DNA binding domain. Mol. Cell. Biol. 19, hdaxx. EMBO Journal 18, Hollenbach, A. D., McPherson, C. J., Lagutina, I., and Grosveld, G. (2002) The EF-hand calcium-binding protein calmyrin inhibits the DNA-binding and transcriptional activity of Pax3. Biochim. Biophys. Acta 1574(3), Hollenbach, A. D., McPherson, C. J., Mientjes, E. J., Iyengar, R. and Grosveld, G. (2002) Daxx and histone deacetylase II associate with chromatin through an interaction with core histones and the chromatin-associated protein Dek J. Cell Sci. 115, Pritchard, C., Grosveld G. and Hollenbach, A. D.* (2003) Alternative splicing of Pax3 produces a transcriptionally inactive protein. Gene 305, Hollenbach, A. D., Hobbs, B. P., and Grosveld, G. (2004) The oncogenic fusion protein Pax3-FKHR inhibits terminal differentiation and enhances apoptosis of primary mouse myoblasts. To be submitted to Mol. Cell. Biol. (* - indicates corresponding author.)

4 GRANT APPLICATIONS The Development of a Yeast One-Hybrid Assay for the Identification of Direct Transcriptional Targets of Pax3 and the Oncogenic Fusion Protein Pax3-FKHR. Cancer Association of Greater New Orleans (CAGNO), funding period 1/1/04 12/31/04. Determining the Role of Phosphorylation in the Promotion of the Solid Muscle Tumor Alveolar Rhabdomyosarcoma. Louisiana State Board of Regents, submitted November 7, The Role of Phosphorylation in the Development of the Solid Muscle Tumor Alveolar Rhabdomyosarcoma. National Institutes of Health, Centers of Biomedical Research Excellence (COBRE) grant, submitted November 18, POSTER PRESENTATIONS Hollenbach, A. D., Sublett, J. E., McPherson, C. J. and Grosveld, G. (1997) Isolation of PAR4, a Novel Pax3 Associated Repressor. Gordon Research Conference on Cancer, Newport, RI, USA. hdaxx. ISREC Conference on Cancer and the Cell Cycle, Lausanne, Switzerland. hdaxx. The Southeast Developmental Biology Meeting, Memphis, TN, USA. Hollenbach, A. D., McPherson, C. J. and Grosveld, G. (1999) Inhibition of Pax3 DNA binding activity by the Pax3 associated calcium binding protein PAPI. Gordon Research Conference on Biological Structure and Gene Expression, Meridin, NH, USA. INVITED PRESENTATIONS The PAX3/FKHR oncoprotein is unresponsive to the PAX3-associated regulator hdaxx. The International Meeting on Forkhead/Winged Helix Proteins, La Jolla, CA, USA. The hdaxx. The Southeast Developmental Biology Meeting, Memphis, TN, USA. The Differential Regulation of Pax3 and the Oncogenic Fusion Protein Pax3- FKHR Through Protein-Protein Interactions. University of Tennessee Medical School, Memphis, TN, USA.

5 Pax3 and Pax3-FKHR in Myogenic Differentiation and Alveolar Rhabdomyosarcoma. Tulane University School of Medicine, Department of Biochemistry, New Orleans, LA, USA. Pax3 and Pax3-FKHR in Myogenic Differentiation and Alveolar Rhabdomyosarcoma. Louisiana State University Health Sciences Center, Department of Cell Biology and Anatomy, New Orleans, LA, USA.