HTGAA Biofabrication and advanced manufaturing

Transcription

1 HTGAA Biofabrication and advanced manufaturing Fiorenzo Omenetto, Benedetto Marelli Silklab,

2 making with natural materials

3 how does nature make? what does nature make with?

4

5

6 synthesis control variables ph viscosity shear concentration water content crystallinity electric field deposition end product

7 solid liquid liquid biopolymers Silk reconstituted silk regenerated silk silk III

8 liquid solid form generation

9 Silk polymorphism forms (I) fibers/waveguides waveguides Advanced Materials 2008 Nature Photonics 2008 Science, 2010 Advanced Materials, 2011 PNAS, 2012 Nature Photonics, 2013 Nature Nano, 2014 PNAS, 2014 PNAS, µm mechanical nanoparticles and nanoshells sheets 10 μm

10 Silk polymorphism forms (II) nanoscale optics opals Λ d Advanced Materials 2008 Nature Photonics 2008 Science, 2010 Advanced Materials, 2011 PNAS, 2012 Nature Photonics, 2013 Nature Nano, 2014 PNAS, 2014 PNAS, 2015 holograms microprism arrays 10 μm

11 Silk polymorphism forms (III) hollow tubes microneedles Advanced Materials 2008 Nature Photonics 2008 Science, 2010 Advanced Materials, 2011 PNAS, 2012 Nature Photonics, 2013 Nature Nano, 2014 PNAS, 2014 PNAS, 2015 mechanical ceramic-like materials

12 Silk polymorphism forms (IV) flexible/conformal RF sensors electronics implantable light Advanced Materials 2008 Nature Photonics 2008 Science, 2010 Advanced Materials, 2011 PNAS, 2012 Nature Photonics, 2013 Nature Nano, 2014 PNAS, 2014 PNAS, 2015 metamaterials resorbable electronics

13 protocols

14 14 Omenetto et al, Silk, 2011

15 ingredients you received delipided and cut silk cocoons what you need salts dialysis membranes syringes needles water (better if DI) oven hot plate

16 Bombyx mori silk cocoons Cut cocoons and dispose of worm Boil cocoons for 30 min in Rinse fibers for 20 min M Na 2 CO 3 times Squeeze out excess water and allow to dry overnight Add 9.3 M LiBr on top of silk fibers and incubate at 60 C for 4 h Add silk/libr to dialysis cassette Dialyze against ultrapure water for 48 h Rockwood et al, Nat. Prot., 2011 Remove silk solution from cassette Centrifuge twice Store at 4 C

17 1. Materiale grezzo Bozzoli di seta 2. Bollire con sali e lavare per rimuovere la sericina (proteina collosa descritta a pag. 14) 3. Dissolvere la seta in un secondo bagno di sali 4. Rimuovere la seta tramite dialisi Risultato: soluzione biocompatibile di pura fibroina e acqua Omenetto et al, Silk, 2011

18 Nonpatterned Add silk to substrate Dry overnight Remove film from substrate Patterned Water anneal for 1 day Rockwood et al, Nat. Prot., 2011

19 Omenetto et al, Silk,

20 Other material options + Fernandez JG, Ingber DE. Unexpected Strength and Toughness in Chitosan-Fibroin Laminates Inspired by Insect Cuticle. Advanced Materials [Internet]. 2012;24:

21 Other material options + Daiwei Ding et al., From Soft Self-Healing Gels to Stiff Films in Suckerin-Based Materials Through Modulation of Crosslink Density and -Sheet Content Advanced Materials - 26 May 2015, DOI: /adma.

22 Cashmere Marelli and Omenetto, J Mater Chem B, 2015

23 Cashmere Marelli and Omenetto, J Mater Chem B, 2015

24 Other material options /03/10/ucla-technology-seminar/ Zhang et al., Ultralightweight and Flexible Silylated Nanocellulose Sponges for the Selective Removal of Oil from Water, Chem. Mater. 2014, 26, pubs.acs.org/doi/pdfplus/ /cm research/projects/polymers-forthe-brain

25 form and function

26 form + function sustainable processed in water controlled degradation edible implantable technological preserves bio-function unique material outcomes dictated by combination of functions above

27 UNIQUE MATERIAL OUTCOMES biological stabilization + bioactive forms enzyme stabilization reagent stabilization flavor stabilization

28 direct mixing of dopants (both organic and inorganic) DxR solution DxR MPA -20 C -20 C 60 C 60 C Au- NP Au nanorods blood BMP-2 antibiotics GFP RhB Rh6G Azobenzene Stilbene QD Porphyrins Enzymes photosystems

29 An example in printing

30 Printing Functional inks printed functions Effective printing area: 8 by 10 letter size inkjet printed objects Development of silk inks Three key things Nozzle voltage Ink temperature Printing waveform

31 Functional inks printed functions biologically active inks no refrigeration needed inkjet printing patterned antibiotics to print drugs on demand active enzyme ink to make surfaces active/reactive/ sensing patterned bone to print patterned living matter Tao, Marelli et al. Advanced Materials, 2015

32 Functional inks printed functions + PDA 2 mm + Antibody + silk UV exposure bacteria exposure

33 Further reading on silk Inkjet printing - H. Tao, B Marelli et al., Inkjet Printing of Regenerated Silk Fibroin: From Printable Forms to Printable Functions, Advanced Materials, doi: /adma , cover story 3D Printing Rod R. Jose, et al., Polyol-Silk Bioink Formulations as Two-Part Room-Temperature Curable Materials for 3D Printing, ACS Biomater. Sci. Eng., 2015, 1 (9), pp doi: /acsbiomaterials.5b00160 Silk Screws Gabriel S. Perrone, et al., The Use of Silk-Based Devices for Fracture Fixation,, Nature Communications, Silk Opals S. Kim, A.N. Mitropoulos, J.D. Spitzberg, H. Tao, D.L. Kaplan, F.G. Omenetto, Silk inverse opals, Nat. Photonics, 6 (2012)