MultiMode 8 Upgrade. Benefits of Upgrading to the High-Performance AFM Benchmark
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1 MultiMode 8 Upgrade Benefits of Upgrading to the High-Performance AFM Benchmark
2 What you will learn How much Multimode evolved: Easy to use Even higher lateral resolution Even more productive / faster scanning How well Multimode is suited for today s and future research on: High-resolution imaging Nano-mechanical modes Nano-electrical modes More questions? Contact me at samuel.lesko@bruker.com 2/22/2017 Bruker Nano Confidential 2
3 Background Current Nanoscope real-time software: 9.2 under Win 7-64 bits Current Nanoscope Analysis: 1.8 under Win XP/7-32 bits or Win 7/10-64 bits Any old Multimode can be upgraded to last brain new system New PC & Controller NS5 New board in base and potentially new Head Upgrade 2/3/2016 Bruker Confidential 3
4 MultiMode AFM Details Bruker Confidential
5 Multimode-8 AFM Highest Resolution Imaging. Any Time, any Sample, every Where Most Complete Quantitative Nano-Mechanical & Nano-Electrical characterization Surprisingly Simple, Making Every User an AFM Expert Bruker has consistently offered upgrades to the MultiMode to extend and improve its capabilities Most used AFM in scientific publications About 50% of all publications using commercial AFMs, used a Multimode AFM 2/22/2017 Bruker Nano Confidential 5
6 Nanoscope-V controller Integrated Signal Access Module 3 Analog Inputs 2 Software controllable Output Line and image TTL signals 3 Lock-in Inputs & Reference signals Digital fast oscilloscope Recording of transient events kHz Up to 50MHz Direct control lock-ins 3 independent Lock-ins Software routable signals 8 channels 3/3/2017 Bruker Confidential 6
7 Gaining lateral resolution Bruker Confidential
8 Ultra High Resolution Any Time, any Sample, every Where, anybody Height Routinely achieve DNA minor groove resolution. Plasmid DNA strand immobilized on mica using low (5 mm) NiCl2 concentration. Major groove, minor groove, and the right-handedness of the helix are clearly visible along the entire strand 0.5 nm 1.5 nm Point defect dimensional and mechanical resolution and not just on hard, flat crystals. Adhesion map of mica in water highlighting differences in the maximum attractive interaction force between individual atoms and the tip Polydiacetylene Organic Monolayer - 5 x 5 nm scan size Bruker Nano Surfaces
9 How to reach this high resolution? Get closer! Sharp tip (& keep it sharp) Resonant mode with tiny amplitude or Peakforce tapping R = 10nm R = 3nm 1.8nm 1nm 1nm 5nm 10nm 2.4nm 9.8nm 17nm 80% VdW force regions 2/22/2017 Bruker Nano Confidential 9
10 How to reach this high resolution? Get the right probe! Advanced applications often require dedicated probes Bruker is the only AFM company with Probe manufacturing & Probe R&D For topography: Higher speed/faster response time Higher resolution 130nm Check 17µm 2/22/2017 Bruker Nano Confidential 10
11 How to reach this high resolution? Get right feedback mode! Carbon nanotube Etched glass Nioprobe tip check Peak Force Tapping Same location & same probe 300nm scan size Tapping 3/3/
12 Gaining ease of use Bruker Confidential
13 Genuine user interface Built-in Experiment database At selection, all default parameters are loaded Simple & Expanded Mode Display only the essential or all parameters for more advanced applications & customization Context-sensitive Help Single-click (F1) access to contact sensitive help & advice Simple workflow Step by step calibration panel with touchless calibration February 11, 2015 Bruker Confidential 13
14 Peak Force Tapping Shorter learning time Direct force control & reading Feedback on peak forces < 20 pn Results in higher resolution, longer tip lifetime, and capability to measure more challenging samples Over 1500 scientific publications Fastest growing new AFM mode Scanasyst Automated parameter optimization in Peakforce Tapping No cantilever tuning required in air & liquid No cantilever Tuning required Easy in both air & liquid 3/3/2017 Bruker Confidential 14
15 PeakForce Tapping Benefits Relative to TappingMode ScanAsyst light TappingMode hard TappingMode 1.5µm scans of poly(butyl acrylate) polymer brush molecules. (Sample courtesy of S. Sheiko, University of North Carolina, Chapel Hill) Bruker s exclusive Peak Force Tapping, directly controls tip-sample interaction forces. This makes it possible to imaging challenging samples that can t be imaged well by conventional AC-mode (tapping) techniques. Here, the sticky side chains of the polymer brush molecules damp the TappingMode oscillation, preventing the tip from reaching the polymer backbone. 3/3/2017 Bruker Nano Surfaces 15
16 PeakForce QNM Eliminates the Uncertainty PFQNM-Height PeakForce QNM can quantitatively and unambiguously identify modulus and adhesion variations where phase imaging cannot. Tapping-Height PFQNM-Adhesion Though phase contrast is often assumed to correlate with modulus variations, both modulus and adhesion actually contribute PeakForce QNM unambiguously measures modulus and adhesion variations, removing any uncertainty about the source of contrast. Tapping-Phase PFQNM-Modulus Multilayered polymer film, 10 µm scans Left: PeakForce QNM Right: TappingMode 16
17 Peak Force TApping Easier operation in Fluid Imaging Designed for the highest resolution fluid imaging with a wide range of dedicated accessories The fluid cell can be used with unique s-shaped o-rings to form a perfusion cell through which fluid can be exchanged using Luer fittings, tubing and syringes Standard EV and JV scanners are highly resistant to fluid damage Bruker guaranteed Standard MultiMode EV and JV scanners are fluid resistant and warranted against fluid damage New PeakForce Tapping High Resolution Fluid probes Fluid cell can be used either as an open cell or a closed perfusion cell Fluid cell O-rings offered in silicone, fluorosilicone and viton for broad range chemical resistance New PeakForce Tapping probes for high resolution fluid imaging 3/3/2017 Bruker Nano Surfaces 17
18 Localizing Chemical Groups on Native Proteins with nanometer resolution PeakForce QNM Adhesion Mapping Resolution of Ni 2+ -NTA probe remains high (3-4nm) compared to unmodified probe (2-3nm). Specific adhesion events detected at distance clearly separate from tip-sample contact region (>4nm). Specific adhesion events were shown to be repeatable in consecutive images (~88.2%). PeakForce Tapping of SAS-6 proteins with unmodified probes. Height Adhesion Overlay PeakForce Tapping of His 6 -Tag engineered SAS-6 proteins with Ni 2+ -NTA modified probes. Adhesion events from 3 consecutive images are shown as red, blue, and green. Pfreundshuh et al. (2014) Nano Lett, vol 14: Bruker Nano Surfaces 3/3/
19 Gaining productivity Bruker Confidential
20 Max scanner size Higher productivity Get on spot Fast navigation through sample (30 seconds per 256x256 pixels images typical) to hunt for right location Collect full data 5k x 5k pixel image on pentacene crystal in less than 2 hours for 50µm scan. Digital zoom over 8µm reveals fine terrace structures (>600x600 pixel image). Create statistics Define automation within the scanner range for multiple automatic image collection together with the self adapting algorithm ScanAsyst to match each local topography 20
21 Higher productivity Experience ScanAsyst-HR in action 1 Hz scan rate, 256x256 5 minutes 10 Hz scan rate, 256x seconds ScanAsyst-Air-HR Probe Tip radius: 10 +/- 5nm Freq: 130 +/- 30 khz k: 0.4 +/- 0.2 N/m Length: 60 µm 21
22 ScanAsyst-HR on biological materials Lambda digest DNA. Scan size 1 4 Hz. Amyloid beta peptide fibrils. Sample clearly shows both twisted mature fibrils and protofibrils. Scan size Hz. (Sample courtesy of A. Lai and J. McLaurin, University of Toronto. Image courtesy of Dr. James Shaw, Bruker.) Bacteriophage: protein capsid with attached tail through which viral DNA is ejected into the bacteria. Scan size 4 Hz. (Sample courtesy of Udom Sae-Ueng and Dr. Alex Evilevitch, Carnegie Mellon University. Image courtesy of James Shaw, Bruker) 3/3/2017 Bruker Nano Surfaces 22
23 Analysis Software Unlimited licenses, free upgrades. Can be installed on other PCs & laptops Fully Featured: Analysis commands: Sections, Steps, Width, Ring, Indentation, Roughness, Depth, Bearing, Filtering: Flatten, Planefit, FFT, filter, erase, Export: JPG, TIFF, Automate analysis sequences 2/22/2017 Bruker Nano Confidential 23
24 Full mechanical suite Bruker Confidential
25 Full force spectroscopy suite Acquisition Time (512x512) 1 day 1 hour 1 minute 10 s Force Spectroscopy Sinus Fast Force Volume Linear Peak Force QNM Ramp rate (Hz) LDPE PS Modulus map of polymer blend (Low Density Polyethylene / Polystyrene) showing dependence versus ramping rate April 14-16, 2015 Bruker Confidential 25
26 Quantitative Mechanical Mapping Faster, more accurate & better lateral resolution Height, Modulus & Adhesion maps of Polydiethylsiloxane (PDES) on Si showing the 3 different materials with high resolution and in normal imaging time (9 min for 512x512 image) Correlation Topo/Force curve 3-compound polymer: hard matrix with rubbery inclusions & semi-crystalline phases. The modulus image & histogram show the 3 materials with high resolution. 3/3/2017 Bruker Confidential 26
27 PeakForce QNM Precise location anytime, anywhere Different amyloid fibril morphologies will vary in rigidity but should have identical Young s modulus. PF-QNM was able to simultaneously: Identify fibril morphology. Quantitatively and accurately measure the modulus independent of fibril morphology (~ kpa). Adamcik et al. (2012) Nanoscale, vol 4: /3/2017 Bruker Nano Surfaces MultiMode 8 AFM operated in air using ScanAsyst Air probes. 27
28 Advanced capabilities in force spectroscopy Force volume from <1Hz to 300 Hz Ramp Rate with up to (256x256) pixels 2048 data points for force curve or (956x956) pixels and 256 data points for force curve Easy calibration interface with touchless deflection sensitivity calibration Advanced modeling models Hertz/DMT Sneddon Cone-Sphere model 3/3/2017 Bruker Nano Surfaces Division 28
29 PeakForce QNM Calibration QNM Calibration within Minutes Significant enhancements to PFQNM Probe calibration Step by Step Wizard Easy for all user types - 4 step routine Run thermal tune Locate sample & engage Measure deflection sensitivity Calibrate QNM at different frequencies Allows for No touch calibration for functionalized probes Requires height sensor 1. Run Thermal Tune 2. Locate Sample & Engage 3. Measure Deflection Sensitivity 4. Calibrate QNM Only takes minutes!! 3/3/2017 Bruker Nano Surfaces 29
30 NanoScope Force Analysis Nanomechanics & Force spectroscopy analysis Complete Force Analysis Wide range of filters and models Automated routines for statistics 3D visualization of Force Data Cubes MATLAB Toolbox for custom analyses Identify true surface with Real Height Visualize Force Data Cubes with Density Plot, Contour Plot, Force Section Soon: Expanded Deformation Models, Adhesion Analysis, Nano-rheology Analysis 3/3/2017 Bruker Nano Surfaces 30
31 Probes Advanced applications often require dedicated probes Bruker is the only AFM company with Probe manufacturing & Probe R&D For nanomechanics: Pre-Calibrated with laser vibrometer Tips with known radius & shape Harder tip materials (diamond) 130nm 17µm 2/22/2017 Bruker Nano Confidential 31
32 Advanced Nano-Electrical Bruker Confidential
33 Electrical characterization PeakForce Tapping Mode current map A V Contact AC + V DC time Peak Force Lift height Force vs. time -1V 2V 2V 0V 0V Peak Current V surface potential map -1V Lift Scan PFT Height Potential Data PeakForce TUNA: PeakForce KPFM: conductivity measurements on fragile samples correlation with nanomechanical properties PFT-topography better suited for KPFM than Tapping Mode (probes, artefacts) correlation with nanomechanical properties
34 Peak Force KPFM Example: Sn-Pb Alloy Correlated electrical & mechanical characterization Height Potential (FM) Adhesion 4x4 µm scan 240 mv 2/22/ Bruker Nano Confidential
35 PeakForce KPFM Repeatability test Test: 9 different probes on same sample Result: 20mV probe-toprobe consistency 2/22/2017 3/3/2017 Bruker Nano Surfaces 35 Division Bruker Nano Confidential 35
36 KPFM observations of corrosion In-situ, over time Delamination of organic coating on carbon steel Topographic images show swelling beneath coating when filament delaminates from the steel substrate Dark areas in surface potential are areas of growing filament acting as anodes, bright areas are acting as cathodes Volta potential from KPFM can be correlated to corrosion potential & used to predict corrosion behavior Topography 0.5hr 2hr cathodes 10hr Potential anodes AISI/SAE 1045 carbon steel with 300nm epoxy coating (90% humidity) Pan, T. et al., Surf. Interface Anal., 45 (6), pp /22/2017 Bruker Nano Confidential Surfaces 36
37 PeakForce TUNA Example Carbon Nanotube Network 2/22/2017 Bruker Nano Confidential 37
38 Conclusion Bruker Confidential
39 Multimode-8 One AFM Many Possibilities General Imaging Modes ScanAsyst TappingMode with Phase Contact Mode and LFM STM One AFM Electrical and Magnetic Properties PeakForce TUNA TUNA, CAFM, SSRM, SCM EFM, KPFM, MFM Life Science and Fluid Imaging ScanAsyst in fluid Small volume fluid cell with perfusion Liquid Resistant Scanners Sample heating, ambient - 60 C Temperature & Environmental Control Heating and Cooling, -35 to 250 C Environmental chambers Many Possibilities Don t see something? Just ask- we can t fit everything on one slide! Material Science / Nanomechanical properties PeakForce QNM Nanoscale Thermal Analysis Piezoresponse microscopy Electrochemistry Glovebox for <1ppm H2O and O2 EC AFM and EC STM Scanning Electrochemical Potential 39
40 Conclusion Peak Force Tapping Ease of use air & fluid Direct & Low force imaging ScanAsyst: Right away expert level image in seamless manner Fast Scanning: statistics & productivity Quantitative Mechanical mapping: accuracy & resolution Advanced Electrical Modes Unique capabilities Accuracy and stability Latest generation NanoScope software: Win7 & offline 2/22/2017 Bruker Nano Confidential 40
41 Interested in seeing the results on your samples? We invite you for a demonstration & have the Multimode-HR tested on your samples Our Team of Application Experts are ready to assist you in solving your AFM challenges, and welcome you in one of our 15 labs worldwide 3/3/2017 Bruker Confidential 41
42 Copyright Bruker Corporation. All rights reserved.
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