Advancements in DNA Construction Tools and Technology

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1 Advancements in DNA Cnstructin Tls and Technlgy Future Bitechnlgy Prducts and Opprtunities t Enhance the Capabilities f the Bitechnlgy Regulatry System Kevin Munnelly President and CEO kmunnelly@gen9bi.cm

2 The Human Genme Prject initiated a cascade f technlgical innvatin and an enrmus amunt f sequence data

3 Rapid Grwth in Base Pairs Recent release f GenBank cntains ver 260,000 rganisms and >190 billin base pairs Rapid grwth in whle genme sequencing will cntinue t expnential grw base pairs in database 3

4 A Massive Divergence in Capacity 3,000x discrepancy READING WRITING Capacity in Base Pairs Per Year 4

5 Synthetic Bilgy and The Big Picture Sustainable energy and materials Fd t feed a grwing glbal ppulatin Therapeutics t fight disease and infectin 5

6 Creating Future Factries Using Synthetic Genes t Create Cellular Factries t Make High Value Prducts Agriculture Therapeutics Bifuels Chemicals Industrial Enzymes 6

7 Uses fr DNA On-Demand single genes* minimal life base pairs genetic circuits genme rewrite 7

8 What is hlding us back? Design build test cycles? Linking DNA design t screening? Ability t build in a brad range f sequence space? 8

9 The Synthetic Bilgy Slutin Using chip technlgy, Gen9 develped a high-thrughput prcess t build DNA at a very high quality and lw cst 9

10 Capacity f Each Chip Over 10 millin base pairs per chip 10

11 Current Prduct Prtfli DNA Synthesis Frm 300bp t as large as 10kb Custm vectr clning Turnarund time in days Industry-leading, vlume-based cnstruct pricing n a per-base basis Variant Libraries Ratinally-Designed High Diversity Variant Libraries 400bp t 1kb up t in diversity Uniquely enabled by the pwer f the BiFab Platfrm 11

12 Experiments Cnducted in Parallel Save Time Cncurrent Cycles RESULTS! TIME Test Design Test Design Test Design Test Design RESULTS! Build Build Build Build Cnsecutive Cycles 12

13 New Design Features and Strategic Prducts Prtein expressin ptimizatin Vectr design tls Yeast clning Lw GC, hmplymer prtcls API fr custmer rdering 10 day turnarund Single gene rdering PCR clning/mdular design suite Pathway sensr cmpnents Pathway design tls Pathway library cnstructin Genme cnstructin and design tls Hst engineering design tls >100kbp synthesis length 13

14 Multiplexing Technlgy Applied t lng DNA cnstructin building 50 gene cnstructs in single reactin Scales DNA synthesis by nearly tw rders f magnitude t billins f base pairs Enables scale dwn f reagents decrease csts t a fractin f a penny per base pair f DNA Technlgy applicatin t BiFab platfrm ver next 12 mnths 14

15 BiFab Platfrm v2.0 MAP Prgram Vlume Pricing $0.03/bp 50+ megabases $0.05/bp 10+ megabases $0.08/bp 2+ megabases Sequences up t 4kb less expensive per/bp than PCR ligs! Gen9 Cnfidential Infrmatin 15

16 Gen9 s BiFab Platfrm is High-Thrughput Design Tls fr Batch Optimizing DNA Sequences Massively Parallel Olignucletide Synthesis Intelligent Executin System fr High-thrughput Assembly Scalable Next-generatin Sequence Verificatin 16

17 What If we culd? Metablize plastics int nutrient rich rganic material? Neutralize Msquit-brn diseases? Prduce nutritius fds in land deemed unfarmable? Generate bisimilars and new therapeutics faster, better cheaper? 17

18 Thank Yu! Our DNA Matters