University Hospital Waterford

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1 University Hospital Waterford Public Health Laboratory, Microbiology Department, University Hospital Waterford, Dunmore Road, Waterford. Testing Laboratory Registration number: 094T is accredited by the Irish National Board (INAB) to undertake testing as detailed in the Schedule bearing the Registration Number detailed above, in compliance with the International Standard ISO/IEC 17025: nd Edition General Requirements for the Competence of Testing and Calibration Laboratories (This Certificate must be read in conjunction with the Annexed Schedule of ) Date of award of accreditation: 05:05:2002 Date of last renewal of accreditation: 27:04:2017 Expiry date of this certificate of accreditation: 27:04:2022 This shall remain in force until further notice subject to continuing compliance with INAB accreditation criteria, ISO/IEC and any further requirements specified by the Irish National Board. Manager: Chairperson: Dr Adrienne Duff Issued on 27 April 2017 Mr Tom O Neill Organisations are subject to annual surveillance and are re-assessed every five years. The renewal date on this Certificate confirms the latest date of renewal of accreditation. To confirm the validity of this Certificate, please contact the Irish National Board. The INAB is a signatory of the European co-operation for (EA) Testing Multilateral Agreement (MLA) and the International Laboratory Cooperation (ILAC) Mutual Recognition Arrangement. Edition 34, 08/03/ T Page 1 of 15

2 Schedule of (Annex to Certificate) UNIVERSITY HOSPITAL WATERFORD Public Health Laboratory Initial Registration Date : Postal Address: (Address of other locations as they apply) 29-June-1998 Microbiology Department University Hospital Waterford Dunmore Road Waterford Telephone: +353 (51) Fax: +353 (51) gerard.doody@hse.ie Contact Name: Gerard Doody Facilities: Public testing service Edition 34, 08/03/ T Page 2 of 15

3 Schedule of THE IRISH NATIONAL ACCREDITATION BOARD (INAB) is the Irish body for the accreditation of organisations including laboratories. Laboratory accreditation is available to testing and calibration facilities operated by manufacturing organisations, government departments, educational institutions and commercial testing/calibration services. Indeed, any organisation involved in testing, measurement or calibration in any area of technology can seek accreditation for the work it is undertaking. Each accredited laboratory has been assessed by skilled specialist assessors and found to meet criteria which are in compliance with ISO/IEC or ISO (medical laboratories). Frequent audits, together with periodic inter-laboratory test programmes, ensure that these standards of operation are maintained. Testing and Calibration Categories: : Permanent laboratory calibration and testing where the laboratory is erected on a fixed location for a period expected to be greater than three years. Category B: Category C: Category D: Site calibration and testing that is performed by staff sent out on site by a permanent laboratory that is accredited by the Irish National Board. Site calibration and testing that is performed in a site/mobile laboratory or by staff sent out by such a laboratory, the operation of which is the responsibility of a permanent laboratory accredited by the Irish National Board. Site calibration and testing that is performed on site by individuals and organisations that do not have a permanent calibration/testing laboratory. Testing may be performed using (a) (b) (c) (d) portable test equipment a site laboratory a mobile laboratory or equipment from a mobile or site laboratory Standard Specification or Test Procedure Used: The standard specification or test procedure that is accredited is the issue that is current on the date of the most recent visit, unless otherwise stated. Glossary of Terms Facilities: Public calibration/testing service: Conditionally available for public calibration/testing: Normally not available for public calibration/testing: Commercial operations which actively seek work from others. Established for another primary purpose but, more commonly than not, is available for outside work. Unavailable for public calibration/testing more often than not. Laboratory users wishing to obtain assurance that calibration or test results are reliable and carried out to the Irish National Board criteria should insist on receiving an accredited calibration certificate or test report. Users should contact the laboratory directly to ensure that this scope of accreditation is current. INAB will, on request, verify the status and scope. Edition 34, 08/03/ T Page 3 of 15

4 Documented in-house methods: on foods Enumeration of Coagulase positive SAF 3V2 based on ISO :1999 &.01 Dairy products staphylococci AMD1: Egg & egg products (Pour plate on selective media).03 Meat and meat products, game and Detection of Salmonella species SAF 9V1 based on ISO :2017 poultry (Selective enrichment/selective.04 Fish, shellfish and molluscs.06 Soups, broths & sauces Enumeration of Escherichia coli ECF 23V2 based on ISO : Cereals and bakery (Surface spread) products.08 Fruit and Vegetables Enumeration of Enterobacteriaceae ENF 33V1 based on ISO : Herbs and spices (Pour plate).10 Non-alcoholic beverages Aerobic plate count at 30 O C, 37 O Cfor PCF 2V3 based on ISO 7218:2007, ISO.13 Ices and desserts 48 hours :2013, ISO 4833:2: Prepared dishes (Spiral plate/ Pour plate).21 Others Detection and enumeration of Listeria LSF 11V2 based on species ISO :2017 & AMD1:2004, ISO :2017 & AMD1:2004 (Selective enrichment & selective.21 Others - Mince meat mechanically separated Aerobic plate count at 30OC, 37OCfor 48 hours PCF 2V3 based on ISO :2013, ISO 4833:2:2013 (Spiral plate/ Pour plate) Edition 34, 08/03/ T Page 4 of 15

5 on foods Enumeration of Clostridium species CPF 6V1 based on ISO 7937 : Dairy products (Surface Spread plate with.02 Egg & egg products Enumeration of Bacillus species and overlay).03 Meat and meat determination of B. cereus products, game and BCF 7V1 based on ISO 7932:2004 poultry (Surface Spread).04 Fish, shellfish and molluscs.06 Soups, broths & sauces.07 Cereals and bakery products.08 Fruit and Vegetables.09 Herbs and spices.10 Non-alcoholic beverages.13 Ices and desserts.17 Prepared dishes.21 Others Documented in-house methods on foods.16 Nuts and nut products, Detection of Salmonella species SAF 9V1 based on ISO :2017 snacks (Selective enrichment & selective.23 Animal feeds Edition 34, 08/03/ T Page 5 of 15

6 Documented in-house methods: on foods Detection of Campylobacter species CAF 17V1 based on ISO Dairy products 1:2017 (Selective enrichment & selective.03 Meat and meat products, game and poultry.07 Cereals and bakery products.09 Herbs and spices.21 Others - Ready to Eat Foods Salads, Desserts eg. Cream cake, Sandwiches, rolls Cook Chill Documented in-house methods: on foods.04 Fish, shellfish and Detection and enumeration of E.Coli in ESF41V1 based on ISO/TS molluscs live bivalve molluscs and other 3: 2005 shellfish (MPN & selective Edition 34, 08/03/ T Page 6 of 15

7 Documented in-house methods: on foods EOF 20 V2 based on ISO.01 Dairy products Detection of E. coli O : Egg & egg products (Selective enrichment, IMS &.03 Meat and meat selective products, game and poultry.04 Fish, shellfish and molluscs.06 Soups, broths & sauces Mayonnaise & sauces.07 Cereals and bakery products.08 Fruit and Vegetables.09 Herbs and spices.10 Non-alcoholic beverages.13 Ices and desserts.17 Prepared dishes.21 Others.23 Animal Feeds Edition 34, 08/03/ T Page 7 of 15

8 on The Microbiology of Drinking Water. foods Methods for the Examination of Waters and Associated Materials parts Non-alcoholic beverages Enumeration of total coliforms CEW1V2 - Part 4 A: 2009 (Membrane filtration & selective Enumeration of E. coli CEW1V2 - Part 4 A: 2009 (Membrane filtration & selective Total bacteria count at 22oC / 72hrs and TCW4V2 - Part 7 A : 2012 (Pour plate) 37oC / 48hrs Enumeration of faecal Streptococci FSW3V1 - Based on ISO 7899:2:2000 (Enterococci) Enumeration of Clostridium perfringens CPW6V1 - Part 6 B:2010 (Membrane filtration & selective Pseudomonas aeruginosa PAW5V1 -" Enumeration of Pseudomonas aeruginosa in water" - Part 8 B: 2010 (Membrane filtration & selective Enumeration of sporulated sulphite- PPC-PATH-MIC-19 - Part 6 A:2010 reducing anaerobes Edition 34, 08/03/ T Page 8 of 15

9 on foods Detection and enumeration of Listeria LSF11V2 based on ISO species 1:2017 & Amd1 :2004, ISO : 2017 &Amd 1: 2004 (Selective enrichment & selective.16 Enumeration of Escherichia coli ECF23V2 based on ISO :2001 (Surface spread).08 Fruit and vegetables Detection of Shiga Toxin-producing PPC-PATH-MIC-38, Detection of Escherichia coli (STEC) stx 1 and stx 2 Shiga Toxin producing E. coli.09 Herbs and spices genes in food (STEC) stx1 and stx2 genes in food based on ISO/TS 13136:2012 and.16 Nuts (Only) ISO/TS 16654:2001 Edition 34, 08/03/ T Page 9 of 15

10 818 for factory hygiene purposes.01 Surfaces Enumeration of bacteria and fungi PPC-PATH-MIC-6 Microbiological environmental monitoring of.02 Air Aseptic Compounding Unit EMF22V1 Environmental monitoring in acute hospital settings ETF8V1 Environmental monitoring.01 Surfaces Identification of Micro-organisms and TM-A-MG005 Identification of Mycology isolates micro-organisms using Matrix.02 Air Assisted Laser Desorption/Ionisation (MALDI) Time of Flight (TOF) Mass Spectrometry. Edition 34, 08/03/ T Page 10 of 15

11 870 Waters, including The Microbiology of Drinking effluents Water. Methods for the Examination of Waters and Associated Materials parts Enumeration of total coliforms CEW 1V2 - Part 4 A condition of potable water.15 Enumeration of Escherichia coli CEW 1V2 - Part 4 A condition of swimming (membrane filtration & selective pools and spas.99 Other Tests Total bacteria count at 22ºC/ 72hrs TCW 4V2 - Part 7 A Hospital Process water and 37ºC/48 hrs (Pour plate) for use in dialysis Sea-shellfish water Enumeration of faecal Streptococci FSW 3V1 Based on ISO 7899:2 Source Water (Enteroccocci) 2000 Sea bathing water isolation) Enumeration of Clostridium perfringens CPW 6V1 - Part 6 B Edition 34, 08/03/ T Page 11 of 15

12 870 Waters, including The Microbiology of Drinking effluents Water. Methods for the.11 Examination of Waters and condition of potable Associated Materials parts water COW7V1 based on ISO Enumeration of total coliforms and E. 2:2012 Water Quality.15 coli by colilert Enumeration of E. Coli and condition of swimming Coliform bacteria Part 2 most pools and spas probable number method.16 Pseudomonas aeruginosa PAW5VI "Enumeration of condition of Pseudomonas aeruginosa in environmental waters water" - Part 8 B Other tests Source water 870 Waters, including Documented in-house methods: effluents LSW8V1 based on ISO : Detection, identification and condition of swimming enumeration of Legionella pools and spas pneumophila type 1,2-14. LSW8V1 based on ISO : condition of Detection, identification and environmental waters enumeration of Legionella sp. (presumptive) Edition 34, 08/03/ T Page 12 of 15

13 Waters, including effluents condition of potable water Other tests Source water Detection of E. coli O157 The Microbiology of Drinking Water. Methods for the Examination of Waters and Associated Materials parts EOW 2V1 - Part 4 F.2009 (Membrane filtration, selective enrichment, IMS & selective. ISO 16654:2001 Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Escherichia coli O157 Water for haemodialysis and related therapies Detection of Salmonella species SSW 10V1 - Part 9 A.2006 (Membrane filtration, selective enrichment & selective. ISO 6579:2002 Microbiology of food and animal feeding stuffs - Horizontal method for the detection of Salmonella spp. Total bacteria count TCW4V2 in house method based on ISO :2009; ISO 23500:2014 & The Microbiology of Drinking Water, Part 7.A) Edition 34, 08/03/ T Page 13 of 15

14 870 Waters, including effluents.99 Other tests Endoscopy Rinse Water Microbiological analysis of Endoscopy rinse waters or other hospital equipment rinse waters Total bacteria count EWW9V1 based on ISO (2006) and ISO (2008) Mycobacterium species EWW9V1 based on ISO (2006) and ISO (2008) Pseudomonas aeruginosa PAW5V1 -" Enumeration of Pseudomonas aeruginosa in water" - Part 8 B: 2010 (Membrane filtration & selective.11 Bacterial condition of potable water Enumeration of Sporulated Sulphite Reducing Anaerobes PPC-PATH-MIC-19 -Part 6 A Others - Marine and fresh recreational waters Enumeration of E.coli in marine and fresh waters using IDEXX Colilert 18 CEW 9V1 based on ISO :2012 Water quality-enumeration of E.coli and colifrom bacteria Part 2: Most probable number method Edition 34, 08/03/ T Page 14 of 15

15 870 Waters, including effluents.11 Bacterial condition of Detection of Shiga Toxin-producing PPC-PATH-MIC-37, Detection of potable wate Escherichia coli (STEC) stx 1 and stx 2 Shiga Toxin producing E. coli genes in water (STEC) stx1 and stx2 genes in water based on ISO/TS 13136:2012 and ISO/TS 16654:2001 Edition 34, 08/03/ T Page 15 of 15

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