Advanced Laboratory Testing Ltd

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1 Ltd Boxer House, Unit 4, Newbridge Industrial Estate, Newbridge, Co. Kildare Testing Laboratory Registration number: 315T is accredited by the Irish National Board (INAB) to undertake testing as detailed in the Schedule bearing the Registration Number detailed above, in compliance with the International Standard ISO/IEC 17025: nd Edition General Requirements for the Competence of Testing and Calibration Laboratories (This Certificate must be read in conjunction with the Annexed Schedule of ) Date of award of accreditation: 10:09:2013 Date of last renewal of accreditation: 10:09:2013 Expiry date of this certificate of accreditation: 10:09:2018 This shall remain in force until further notice subject to continuing compliance with INAB accreditation criteria, ISO/IEC and any further requirements specified by the Irish National Board. Manager: Dr Adrienne Duff Chairperson: Issued on 10 September 2013 Mr Tom O Neill Organisations are subject to annual surveillance and are re-assessed every five years. The renewal date on this Certificate confirms the latest date of renewal of accreditation. To confirm the validity of this Certificate, please contact the Irish National Board. INAB is a signatory of the European co-operation for (EA) Multilateral Agreement (MLA) and the International Laboratory Cooperation (ILAC) Mutual Recognition Arrangement, for Testing. Edition 15, 11/01/ T Page 1 of 25

2 Schedule of (Annex to Certificate) ADVANCED LABORATORY TESTING LTD Biological and Chemical Testing Laboratory Initial Registration Date : Postal Address: (Address of other locations as they apply) 10-September-2013 Biological Testing Laboratory Boxer House Unit 4 Newbridge Industrial Estate Newbridge Co. Kildare Chemical Testing Laboratory Unit T M7 Business Park Newhall Naas Co. Kildare Telephone: +353 (45) Contact Name: Facilities: grahamohalloran@altesting.ie Graham O'Halloran Public testing service Edition 15, 11/01/ T Page 2 of 25

3 Schedule of THE IRISH NATIONAL ACCREDITATION BOARD (INAB) is the Irish body for the accreditation of organisations including laboratories. Laboratory accreditation is available to testing and calibration facilities operated by manufacturing organisations, government departments, educational institutions and commercial testing/calibration services. Indeed, any organisation involved in testing, measurement or calibration in any area of technology can seek accreditation for the work it is undertaking. Each accredited laboratory has been assessed by skilled specialist assessors and found to meet criteria which are in compliance with ISO/IEC or ISO/IEC (medical laboratories). Frequent audits, together with periodic inter-laboratory test programmes, ensure that these standards of operation are maintained. Testing and Calibration Categories: : Permanent laboratory calibration and testing where the laboratory is erected on a fixed location for a period expected to be greater than three years. Category B: Category C: Category D: Site calibration and testing that is performed by staff sent out on site by a permanent laboratory that is accredited by the Irish National Board. Site calibration and testing that is performed in a site/mobile laboratory or by staff sent out by such a laboratory, the operation of which is the responsibility of a permanent laboratory accredited by the Irish National Board. Site calibration and testing that is performed on site by individuals and organisations that do not have a permanent calibration/testing laboratory. Testing may be performed using (a) (b) (c) (d) portable test equipment a site laboratory a mobile laboratory or equipment from a mobile or site laboratory Standard Specification or Test Procedure Used: The standard specification or test procedure that is accredited is the issue that is current on the date of the most recent visit, unless otherwise stated. Glossary of Terms Facilities: Public calibration/testing service: Conditionally available for public calibration/testing: Normally not available for public calibration/testing: Commercial operations which actively seek work from others. Established for another primary purpose but, more commonly than not, is available for outside work. Unavailable for public calibration/testing more often than not. Laboratory users wishing to obtain assurance that calibration or test results are reliable and carried out to the Irish National Board criteria should insist on receiving an accredited calibration certificate or test report. Users should contact the laboratory directly to ensure that this scope of accreditation is current. INAB will, on request, verify the status and scope. Edition 15, 11/01/ T Page 3 of 25

4 Biological Testing Laboratory Microbiological tests on foods Egg and egg Meat and meat Detection of Salmonella spp by selective enrichment technique Detection of Salmonella spp using Solus method MTM001: ISO 6579:2002 (A1:2007) & ISO 6785: 2007 MTM002: Solus Salmonella ELISA test Confirmation of Salmonella spp MTM003: ISO 6579:2002, game and poultry Detection of Listeria spp by selective enrichment technique MTM004: ISO :1997 (A1:2004) Fish, shellfish and molluscs Fats and oils Soups, broths and sauces Fruit and vegetables Herbs and spices Non-alcoholic beverages Alcoholic beverages Detection of Listeria spp using Solus ELISA method MTM005: Solus Listeria ELISA test Confirmation of Listeria spp MTM006: ISO :1997 (A1:2004) Detection and confirmation of thermotolerant Campylobacter spp by selective enrichment technique Detection and confirmation of E. coli 0157 MTM007: ISO :2006 MTM008: ISO 16654: (other than wine) Ices and desserts Confectionery Detection and confirmation of E. coli 0157:H7 using Reveal test system MTM009: Reveal test system.16 Nuts and nut, snacks Enumeration of microorganisms: aerobic colony count (30 C for 48 hours) MTM010a: ISO : Additives Pet foods Animal feeds Enumeration of microorganisms: aerobic colony count (30 C for 72 hours) Enterobacteriaceae MTM010b: ISO :2013 MTM011: ISO :2004 Edition 15, 11/01/ T Page 4 of 25

5 Biological Testing Laboratory Microbiological tests on foods Egg and egg coliforms E. coli MTM012:ISO 4832:2006 MTM013a: ISO :2001 Meat and meat, game and E. coli MTM013b: ISO :2001 poultry Fish, shellfish and molluscs of coagulase positive staphylococci MTM014: ISO : Fats and oils Soups, broths and sauces of Bacillus cereus Enumeration of Listeria monocytogenes and Listeria spp MTM015: ISO 7932:2005 MTM016: ISO :1998 (A1:2004) Fruit and vegetables Herbs and spices Pseudomonas spp MTM017: ISO 13720: Non-alcoholic beverages Alcoholic beverages of Clostridium perfringens MTM018: ISO 7937: (other than wine) Ices and desserts Confectionery faecal streptococci (enterococci) MTM021: BS 4285:1985 section Nuts and nut, snacks Additives yeasts and moulds in food and swabs with an Aw greater than 0.95 MTM022: ISO : Pet foods Animal feeds Detection of Listeria SPP using ALOA One Day Method MTM036:ALOA ONE DAY TM (Detection) AFNOR Validation Certificate N 0 AES 10/3-09/00 Edition 15, 11/01/ T Page 5 of 25

6 Biological Testing Laboratory 811 Microbiological tests on foods yeasts and moulds in food and swabs with an Aw less than 0.95 MTM023: ISO : Egg and egg Meat and meat, game and poultry.06 Fish, shellfish and molluscs Soups, broths and sauces Fruit and vegetables Herbs and spices Ices and desserts Confectionery Nuts and nut, snacks Additives Pet foods Animal feeds Edition 15, 11/01/ T Page 6 of 25

7 Biological Testing Laboratory 811 Microbiological tests on foods of lactic acid bacteria MTM019: ISO 15214:1988 Meat and meat, game and of Lactobacillus species MTM020: Oxoid manual (online) poultry Meat and meat, game and poultry Enumeration of microorganisms: anaerobic colony count MTM030: Documented inhouse method Detection of coliforms MTM031: ISO 4831:2006 Meat and Meat and Meat game and poultry Detection and confirmation of Cronobacter spp (formally Enterobacter sakazakii) MTM040 Rapid Sakazakii ANOR validation Certificate N BRD 07/22-05/12 Meat and meat, game and poultry Detection and confirmation of E. coli O157 using VIDAS UP E. coli O157 Test MTM035: BIOMERIEUX VIDAS UP E. coli O157 (including H7) (ECPT) AFNOR VALIDATION Certificate N BIO 12/25-05/09 Meat and meat, game and poultry ENUMERATION OF CAMPYLOBACTER SPECIES MTM041: ENUMERATION OF CAMPYLOBACTER SPECIES based on ISO :2006 Edition 15, 11/01/ T Page 7 of 25

8 Biological Testing Laboratory Microbiological tests on foods Egg and egg Meat and meat, game and poultry Fish, shellfish and molluscs Fats and oils Soups, broths and sauces Fruit and vegetables Herbs and spices Non-alcoholic beverages Alcoholic beverages (other than wine) Ices and desserts Confectionery Nuts and nut, snacks Additives Pet foods Animal feeds Detection of Salmonella spp using Real-Time PCR Detection of Listeria spp using Real-Time PCR DETECTION OF SALMONELLA SPP BY IBISA RAPID CULTURE METHOD MTM037: BIORAD iq- CheckTM Salmonella II. AFNOR VALIDATION Certificate N BRD 07/06-07/04 MTM038: BIORAD iq- CheckTM Listeria spp. AFNOR VALIDATION Certificate N BRD 07/13-05/07 MTM047: IBISA Selective medium for the detection of Salmonella AFNOR VALIDATION Certificate N AES 10/11 07/11 Edition 15, 11/01/ T Page 8 of 25

9 Biological Testing Laboratory 811 Microbiological tests on foods Meat and meat, game and poultry DETECTION, IDENTIFICATION AND CONFIRMATION OF SHIGA TOXIN-PRODUCING ESCHERICHIA COLI O26, O45, O103, O111, O121, O145 and O157:H7 (STEC) USING REAL- TIME PCR MTM042A BIORAD iq-check TM STEC VirX Real-Time PCR. AOAC Certificate reference: MTM042B iq-check TM STEC SerO Real-Time PCR. AOAC Certificate reference: USDA FSIS MLG 5B.05 ISO/TS 13136: Testing of surfaces in abattoirs Meat surfaces Product contact surfaces Detection of Listeria SPP using ALOA One Day Method MTM036: ALOA ONE DAY TM(Detection) AFNOR Validation Certificate N0 AES 10/3-09/00 Detection of Salmonella spp using Real-Time PCR MTM037: BIORAD iq-check TM Salmonella II. AFNOR VALIDATION Certificate N BRD 07/06-07/04 Detection of Listeria spp using Real-Time PCR MTM038: BIORAD iq-check TM Listeria spp. AFNOR VALIDATION Certificate N BRD 07/13-05/07 Edition 15, 11/01/ T Page 9 of 25

10 Biological Testing Laboratory Testing of surfaces in abattoirs Meat surfaces Product contact surfaces DETECTION, IDENTIFICATION AND CONFIRMATION OF SHIGA TOXIN-PRODUCING ESCHERICHIA COLI O26, O45, O103, O111, O121, O145 and O157:H7 (STEC) USING REAL-TIME PCR MTM042A BIORAD iq-check TM STEC VirX Real-Time PCR. AOAC Certificate reference: MTM042B iq-check TM STEC SerO Real-Time PCR. AOAC Certificate reference: USDA FSIS MLG 5B.05 ISO/TS 13136: Testing of surfaces in abattoirs Detection of Salmonella spp by selective enrichment technique MTM001: ISO 6579:2002 (A1:2007) & ISO 6785: Meat surfaces Product contact surfaces Detection of Salmonella spp using Solus method MTM002: Solus Salmonella ELISA test Confirmation of Salmonella spp MTM003: ISO 6579:2002 Detection of Listeria spp MTM 004: ISO :1997 (A1:2004) Detection of Listeria spp using Solus ELISA method MTM 005: Solus Listeria ELISA test Confirmation of Listeria spp MTM006: ISO :1997 (A1:2004) Detection and confirmation of E. coli 0157 MTM008: ISO 1654:2001 Edition 15, 11/01/ T Page 10 of 25

11 Biological Testing Laboratory Testing of surfaces in abattoirs Meat surfaces Product contact surfaces Detection and confirmation of E. coli 0157:H7 using Reveal test system Enumeration of aerobic colony count (30 C for 48 hours) Enumeration of aerobic colony count (30 C for 72hours) Enterobacteriaceae coliforms MTM009: Reveal test system MTM010a: ISO :2013 MTM010b: ISO :2013 MTM011: ISO :2004 MTM012: ISO 4832: Testing of surfaces in abattoirs E. coli MTM013a: ISO : Meat surfaces Product contact E. coli MTM013b: ISO :2001 surfaces of coagulase positive staphylococci MTM014: ISO :1999 of Bacillus cereus MTM015: ISO 7932: 2005 Enumeration of Listeria monocytogenes and Listeria spp MTM016: ISO :1998 (A1:2004) Pseudomonas spp MTM017: ISO 13720:2010 Edition 15, 11/01/ T Page 11 of 25

12 Biological Testing Laboratory Testing of surfaces in abattoirs Meat surfaces Product contact surfaces of Clostridium perfringens faecal streptococci (enterococci) yeasts and moulds in food and swabs with an Aw greater than 0.95 Detection of Salmonella spp from carcass swabs using a USDA compliant method MTM018: ISO 7937:2004 MTM021: BS 4285:1985 Section 3.11 MTM22: ISO :2008 MTM033: MLG 4.08 Enumeration of coliforms and Escherichia coli in carcass swabs using a USDA compliant method MTM034: Association of Official Analytical Chemists (AOAC) official method Microbiological tests for factory hygiene purposes Surfaces DETECTION OF SALMONELLA SPP BY IBISA RAPID CULTURE METHOD Detection of Salmonella spp by selective enrichment technique Detection of Salmonella spp using Solus method MTM047: IBISA Selective medium for the detection of Salmonella AFNOR VALIDATION Certificate N AES 10/11 07/11 MTM001: ISO 6579:2002 (A1:2007) & ISO 6785: 2007 MTM002: Solus Salmonella ELISA test Confirmation of Salmonella spp MTM003: ISO 6579:2002 Detection of Listeria spp using selective enrichment technique MTM004: ISO :1997 (A1:2004) Edition 15, 11/01/ T Page 12 of 25

13 Biological Testing Laboratory 818 Microbiological tests for factory hygiene purposes Surfaces Detection of Listeria spp using Solus ELISA method MTM005: Solus Listeria ELISA test Confirmation of Listeria spp MTM006: ISO :1997 (A1:2004) Detection and confirmation of E. coli 0157 Detection and confirmation of E. coli 0157:H7 using Reveal test system Enumeration of microorganisms: aerobic colony count (30 C for 48 hours) Enumeration of microorganisms: aerobic colony count (30 C for 72 hours) Enterobacteriaceae coliforms MTM008: ISO 16654:2001 MTM009: Reveal test system MTM010a: ISO :2013 MTM010b: ISO :2013 MTM011: ISO :2004 MTM012: ISO 4832: Microbiological tests for factory hygiene purposes Surfaces E. coli E. coli of coagulase positive staphylococci of Bacillus cereus MTM013a: ISO :2001 MTM013b: ISO :2001 MTM014: ISO :1999 MTM015: ISO 7932: 2205 Edition 15, 11/01/ T Page 13 of 25

14 Biological Testing Laboratory 818 Microbiological tests for factory hygiene purposes Surfaces Enumeration of Listeria monocytogenes and Listeria spp Pseudomonas spp of Clostridium perfringens faecal streptococci (enterococci) yeasts and moulds in food and swabs with an Aw greater than 0.95 MTM016: ISO :1998 (A1:2004) MTM017: ISO 13720:2010 MTM018: ISO 7937:2004 MTM021: BS 4285:1985 Section 3.11 MTM022: ISO :2008 Detection of Listeria SPP using ALOA One Day Method Detection and confirmation of Cronobacter spp (formally Enterobacter sakazakii) Detection of Salmonella spp using Real-Time PCR Detection of Listeria spp using Real-Time PCR MTM036: ALOA ONE DAY TM(Detection) AFNOR Validation Certificate N0 AES 10/3-09/00 MTM040 Rapid Sakazakii ANOR validation Certificate N BRD 07/22-05/12 MTM037: BIORAD iq-check TM Salmonella II. AFNOR VALIDATION Certificate N BRD 07/06-07/04 MTM038: BIORAD iq-check TM Listeria spp. AFNOR VALIDATION Certificate N BRD 07/13-05/07 DETECTION OF SALMONELLA SPP BY IBISA RAPID CULTURE METHOD MTM047: IBISA Selective medium for the detection of Salmonella AFNOR VALIDATION Certificate N AES 10/11 07/11 Edition 15, 11/01/ T Page 14 of 25

15 Biological Testing Laboratory 870 Waters, including effluents.11 Bacteriological condition of potable waters.12 Bacteriological condition of industrial waters (treated, recirculating).14 Bacteriological condition of trade wastes.15 Bacteriological condition of swimming pools and spas.16 Bacteriological condition of environmental waters Enumeration of colony count in water of coliforms and E. coli in water of Pseudomonas aeruginosa in water of enterococci in water of Clostridium perfringens in water Analysis of water for total viable count (HTM 2030) MTM024: "The Microbiology of Drinking Water" 2012, Part 7 MTM025: "The Microbiology of Drinking Water" 2009, Part 4 MTM026: "The Microbiology of Drinking Water" 2010, Part 8 MTM027: "The Microbiology of Drinking Water" 2012, Part 5 MTM028: "The Microbiology of Drinking Water" 2010, Part 6 MTM029: Health Technical Memorandum 2030, 1997 Edition 15, 11/01/ T Page 15 of 25

16 Unit T, M7 Business Park, Newhall, Naas, Co. Kildare Chemical Testing Laboratory 751 Chemical Tests on Foods Meat and meat, game and poultry Determination of Nitrate, Nitrite and Salt using Anion Exchange Chromatography 7-275mg/kg NaNO 3 and mg/kg as NaNO 2 by IC Salt: 0.1-6g/100g FCTM005: Dionex application note 112, 1998 ISO 2918(1975) Meat and meat -Determination of Nitrite content ISO 3091 (1975) Meat and meat - Determination of Nitrate content, ISO 1841 (1996) Meat and meat - Determination of Chloride content.06 Meat and meat, game and poultry Fish, shellfish and molluscs Soups, broths and sauces DETERMINATION OF TOTAL FAT AND MOISTURE IN FOODS Range: Moisture % Fat: % Powders % Low dairy % FCTM006 based on AOAC Official Method ( , Chapter 39 p. 27). Using CEM NMR Analyser..08 Fruit and vegetables Dairy 10-85%.15 Confectionery Meat Low 0.1-4%.16 Nuts and nut, snacks Meat 4-30% Sauce %.22 Pet Foods Edition 15, 11/01/ T Page 16 of 25

17 Unit T, M7 Business Park, Newhall, Naas, Co. Kildare Chemical Testing Laboratory.06 Meat and meat, game and poultry Fish, shellfish and molluscs Soups, broths and sauces DETERMINATION OF ASH IN FOODS Range: g/100g FCTM007 based on AOAC Official method ( , Chapter 9, p. 19) using CEM Microwave Furnace..08 Fruit and vegetables.15 Confectionery.16 Nuts and nut, snacks.22 Pet Foods.06 Meat and meat, game and poultry Fish, shellfish and molluscs Soups, broths and sauces DETERMINATION OF SODIUM (SALT) IN FOODS Range: g/100g FCTM008 based on AOAC Official Method ( , Chapter 50 p. 65). AOAC Official Method , (Chapter 27 p. 21). Using Atomic Absorption Spectrometry Fruit and vegetables Confectionery Nuts and nut, snacks DETERMINATION OF SODIUM (SALT) IN FOODS Range: g/100g FCTM008 based on AOAC Official Method ( , Chapter 50 p. 65). AOAC Official Method , (Chapter 27 p. 21). Using Atomic Absorption Spectrometry. Edition 15, 11/01/ T Page 17 of 25

18 Unit T, M7 Business Park, Newhall, Naas, Co. Kildare Chemical Testing Laboratory.06 Meat and meat, game and poultry Fish, shellfish and molluscs Soups, broths and sauces DETERMINATION OF TOTAL SUGARS IN FOOD Range: g/100g FCTM009 based on Pearson s Composition and Analysis of Foods 9th Edition, Ronald S Kirk and Ronald Sawyer (page 198). Official Journal Of The European Union, COMMISSION REGULATION (EC) No 152/2009 Using HPLC..08 Fruit and vegetables.15 Confectionery.16 Nuts and nut, snacks Meat and meat, game and poultry Fish, shellfish and molluscs Soups, broths and sauces DETERMINATION OF NITROGEN (PROTEIN) IN FOOD Range: g/100g (%) FCTM010 AOAC Official Method ( , Chapter 39, p.7). Using Leco Nitrogen Analyser Fruit and vegetables Confectionery Nuts and nut, snacks DETERMINATION OF NITROGEN (PROTEIN) IN FOOD Range: g/100g (%) FCTM010 AOAC Official Method ( , Chapter 39, p.7). Using Leco Nitrogen Analyser. Edition 15, 11/01/ T Page 18 of 25

19 Unit T, M7 Business Park, Newhall, Naas, Co. Kildare Chemical Testing Laboratory.22 Pet Foods.06 Meat and meat, game and poultry Fish, shellfish and molluscs Soups, broths and sauces DETERMINATION OF FATTY ACID COMPOSITION IN FOODS Range: Saturated fatty Acids Mono unsaturated Fatty Acids Poly unsaturated Fatty Acids 0.1g/100g FCTM011 AOAC Official method ( , Chapter 41, p.27). Using GLC. Fruit and vegetables Confectionery Nuts and nut, snacks.16 Meat and meat, game and poultry Fish, shellfish and molluscs Soups, broths and sauces DETERMINATION OF TOTAL DIETARY FIBRE IN FOODS Range: g/100g FCTM012 Official AOAC method (32.1, Chapter 32, p.7). Using ANKOM Analyser..06 Edition 15, 11/01/ T Page 19 of 25

20 Unit T, M7 Business Park, Newhall, Naas, Co. Kildare Chemical Testing Laboratory Fruit and vegetables Confectionery Nuts and nut, snacks.06 Meat and meat, game and poultry Fish, shellfish and molluscs Soups, broths and sauces ENERGY CALCULATIONS FOR FOODS FCTM013 Regulation (EU) No 1169/2011 Of The European Parliament And Of The Council. Pearson s Composition and Analysis of Foods 9th Edition, Ronald S Kirk and Ronald Sawyer, (page 5). Fruit and vegetables Confectionery Nuts and nut, snacks ENERGY CALCULATIONS FOR FOODS FCTM013 Regulation (EU) No 1169/2011 Of The European Parliament And Of The Council. Pearson s Composition and Analysis of Foods 9th Edition, Ronald S Kirk and Ronald Sawyer, (page 5). Edition 15, 11/01/ T Page 20 of 25

21 Unit T, M7 Business Park, Newhall, Naas, Co. Kildare Chemical Testing Laboratory Meat and meat, game and poultry CALCULATIONS FOR FOODS FCTM014A Added Water. FCTM014B Apparent Total Meat Content by Stubbs and Moore method. Regulation (EU) No 1169/2011 on EU provision of Food Information for consumers Regulation. Ronald S Kirk and Ronald Sawyer - Pearson s Composition and Analysis of Food, 9th Edition. (Chapter 13) Meat and meat - the calculation of meat content, added water and connective tissue from analytical data 2nd Ed 2007 (Campden BRI) Royal Society of Chemistry, AMC technical briefs Meat and Poultry Nitrogen factors 2014 Edition 15, 11/01/ T Page 21 of 25

22 Unit T, M7 Business Park, Newhall, Naas, Co. Kildare Chemical Testing Laboratory Waters Waters for potable and domestic purposes Sewage Trade wastes Other Waters Determination of Chloride, ammonium, nitrite, total oxidised nitrogen (TON), nitrate, alkalinity, hardness, sulphate, orthophosphate using gallery autoanalyser Alkalinity as CaCO mg/L Ammonia as NH 3 -N mg/l Chloride as Cl mg/L Hardness as CaCO mg/l Nitrite as N 0-10mg/L Orthophosphate as P 0-50mg/L Sulphate as SO mg/L TON 1-110mg/L Nitrate as N 1-100mg/L ECTM001A: Thermo- Scientific Method I.D. ALKBpB, Issue 003, 01 ECTM001B: Thermo- Scientific Method ID AMMDIC, Issue 002, ECTM001C: Thermo- Scientific Method ID CHLOR, Issue 002, ECTM001D: Thermo- Scientific Method ID HAR001, Issue 002, ECTM001E:Thermo-Scientific Method ID NITRI, Issue 002, ECTM001F: Thermo- Scientific Method ID PHOS, Issue 002, ECTM001G: Thermo- Scientific Method ID SULP, Issue 002, ECTM001H: Thermo- Scientific Method ID TON, Issue 002, Edition 15, 11/01/ T Page 22 of 25

23 Unit T, M7 Business Park, Newhall, Naas, Co. Kildare Chemical Testing Laboratory 766 Waters Waters for potable and domestic purposes Sewage Determination of Electrical Conductivity ECTM002: Standard Methods for the Examination of Water and Waste water, Method 2510, 22 nd Edition, APHA, Trade wastes.99 Other Waters 766 Waters Sewage Determination of Suspended Solids using Gravimetry mg/L ECTM003: Standard Methods for the Examination of Water and Waste water, Method 2540, 22 nd Edition, APHA, Trade wastes.99 Other Waters Sewage Trade wastes Other Waters Determination of 5 day Biochemical demand (BOD) using dissolved Oxygen Meter mg/L O 2 ECTM004: Methods for the Examination of Waters and associated materials, HMSO 1988 ISBN , APHA 21st edition 2005, Standard Methods for the analysis of water and waste water 5210 and 4500-C, HACH user manual DOC , Edition 2, 2013 Edition 15, 11/01/ T Page 23 of 25

24 Unit T, M7 Business Park, Newhall, Naas, Co. Kildare Chemical Testing Laboratory Waters Sewage Trade wastes Determination of Chemical Oxygen Demand (Dichromate Value) Low-range ( 5-150mg/LO 2 ) Mid-range ( mg/LO 2 ) High-range ( mg/LO 2 ) ECTM005: Standard Methods for the Examination of Water and Wastewater, APHA, Method 5220 D, 22 nd Edition, Others Waters 766 Waters Waters for potable and domestic purposes Determination of ph using Orion Star A211 ph 4-10 Ectm007: Standard Methods for the Examination of Water and wastewater, Method 4500, 22 nd Edition, APHA, 2012 Sewage.05 Trade wastes.99 Other Waters Edition 15, 11/01/ T Page 24 of 25

25 Unit T, M7 Business Park, Newhall, Naas, Co. Kildare Chemical Testing Laboratory Waters Sewage Trade waters Determination of Total Phosphorus mg/L P ECTM008: Standard Methods for the Examination of water and wastewater Method 4500-E, 22 nd Edition, APHA, 2012, and HACH method 8190 Edition, 8, 04/ Other Waters 766 Waters Sewage.05 Trade waters.99 Other waters Determination of Total Nitrogen and Kjeldahl Nitrogen Total Nitrogen 1.0 to 200 mg/l N Kjeldahl Nitrogen (Calculation) ECTM006: Method in HACH water analysis Handbook Edition 15, 11/01/ T Page 25 of 25

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