HICHROM. Chromatography Columns and Supplies. LC COLUMNS ES Industries. Catalogue 9. Hichrom Limited

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1 HICHROM Chromatography Columns and Supplies LC COLUMNS ES Industries Catalogue 9 The Markham Centre, Station Road Theale, Reading, Berks, RG7 4PE, UK Tel: +44 (0) Fax: +44 (0) sales@hichrom.co.uk

2 ES INDUSTRIES ES Industries ES Industries, based in the north-east of the USA, have been manufacturing HPLC columns for over 0 years. A number of their more popular products are discussed. Epic Ultra high purity spherical silica High density bonding Microbore to preparative dimensions Epic is ES Industries newest range of HPLC columns, based on high density bonded monomeric phases produced through a proprietary bonding process. The Epic series is compatible with a wide range of organic modifiers and buffers and stable over a wide ph range. Whereas the Epic C8-MS phase is specifically designed for LC-MS, Epic C8-SD is a super dense, highly hydrophobic and exceptionally inert phase for the analysis of both acids and bases. Epic Polar can be used with 00% aqueous eluents and is suitable for the analysis of polar analytes. Epic Phases Epic Phase Particle Size (μm) Pore Size (Å) Surface Area (m /g) Carbon Load (%) ph Range C8.8,, 5, C8.8,, 5, C8-MS.8,, 5, C8-SD.8,, 5, C4-SD.8,, 5, Polar.8,, 5, PFP-LB.8,, 5, N/A - 0 FO-LB.8,, 5, N/A - 0 HILIC-HC.8,, 5, N/A - 0 Phenyl-Hexyl.8,, 5, SCX.8,, 5, N/A - 0 The Epic PFP-LB phase is bonded with pentafluorophenyl groups and is ideal for LC-MS, due to low bleed and stabilised baseline. It is suitable for the separation of aromatics, polar compounds, halogenated compounds, isomers and natural products. The Epic FO-LB is a similarly baseline stabilised, alkyl perfluorinated C8 phase, which is well suited to the analysis of halogenated analytes and lipophilic compounds. Epic HILIC-HC (High Capacity) is designed for the LC-MS analysis of polar compounds. It is composed of a polyhydroxylated polymer, coated and bound to silica. This composition provides hydroxyl levels that are well above conventional diol type stationary phases. Figure illustrates the retention of uracil and cytosine on this HILIC column. Column: Epic HILIC-HC (5µm, 50 x 4.6mm) Eluent: CH CN - 6mM ammonium acetate (80:0) Flow rate: 0.5ml/min Figure. Epic HILIC-HC for retention of uracil and cytosine. Toluene. Uracil. Cytosine Mins For free technical advice and support technical@hichrom.co.uk Column Dimensions (mm) 5μm Epic Phase Guard Cartridges 50 x x x x 4.6 (5/pk) C8 59-EC8 59-EC8 59-EC8 559-EC8 000-EC8 C8 59-EC8 59-EC8 59-EC8 559-EC8 000-EC8 C8-MS 59-EC8-MS 59-EC8-MS 59-EC8-MS 559-EC8-MS 000-EC8-MS C8-SD 59-EC8-SD 59-EC8-SD 59-EC8-SD 559-EC8-SD 000-EC8-SD C4-SD 59-EC4-SD 59-EC4-SD 59-EC4-SD 559-EC4-SD 000-EC4-SD (POA) Polar 59-EPO 59-EPO 59-EPO 559-EPO 000-EPO PFP-LB 59-EPFP-LB 59-EPFP-LB 59-EPFP-LB 559-EPFP-LB 000-EPFP-LB FO-LB 59-EFO-LB 59-EFO-LB 59-EFO-LB 559-EFO-LB 000-EFO-LB HILIC-HC 59-EHIC 59-EHIC 59-EHIC 559-EHIC 000-EHIC Phenyl-Hexyl 59-EPHX 59-EPHX 59-EPHX 559-EPHX 000-EPHX (POA) SCX 59-ESCX (POA) 59-ESCX (POA) 59-ESCX (POA) 559-ESCX (POA) 000-ESCX (POA) Other particle sizes available Other column dimensions available Use with guard cartridge holder 0000 ( 87) and column coupler 0006 ( 9) Please contact Hichrom for ordering information for alternative particle sizes or column dimensions. 00

3 AquaSep TM Separates polar compounds with 00% aqueous eluents Rapid re-equilibration with gradients (0-00%) No ion-pairing reagents required Patented single step bonding AquaSep Phase Particle Size (μm), 5 Pore Size (Å) 00 Surface Area (m /g) 450 Carbon Load (%) 6 ph Range - 8 AquaSep TM was developed using patented technology for use with high aqueous content eluents. In order to obtain high aqueous stability and maximum hydrophobic interaction, AquaSep contains an ether linkage near the point of attachment to the silica base. This allows water to penetrate the surface and prevents phase collapse. AquaSep can retain highly water soluble compounds such as small organic acids (Figure ). 4 5 Figure. Separation of organic acids 6 Column: AquaSep (50 x 4.6 mm) Eluent: 0.05M KH PO 4, ph.4 Flow rate: ml/min. Oxalic acid. Tartaric acid. Formic acid 4. Malic acid 5. Malonic acid 6. Lactic acid 7. Maleic acid 8. Fumaric acid Mins 7 8 AquaSep Length Internal Diameter (mm) Particle Size (μm) (mm) Price 50 -AQS d-aqs 5-AQS AQS d-aqs 5-AQS AQS d-aqs 5-AQS AQS d-aqs 5-AQS AQS d-aqs 5-AQS AQS d-aqs 5-AQS AQS 5d-AQS 55-AQS 64 Use with guard cartridge 000-AQS ( ), guard cartridge holder 0000 ( 87) and column coupler 0006 ( 9) FluoroSep TM -RP FluoroSep TM -RP is a family of reversed-phase fluorinated ligands bonded to high efficiency silica. FluoroSep-RP columns may be used with all reversed-phase eluents, buffers and ion-pairing reagents. The phases are stable from ph to 8 and may be heated to 80 C. Three phases are available. The wide pore 00Å FluoroSep-RP Propyl is suitable for the separation of proteins and peptides. FluoroSep-RP Phases Phase Functional Group Particle Size (μm) Pore Size (Å) Surface Area (m /g) ph Range Phenyl (FSP) Pentafluorophenyl, (FSP), 450 (FSP/HS) - 8 Octyl (FO) Perfluorooctyl Propyl (FP) Perfluoropropyl FluoroSep-RP Phenyl (FSP) is used for the separation of closely related geometrical isomers, particularly aromatic compounds, halogenated compounds, conjugated systems and epimers. A high surface area FSP/HS material is also available. FluoroSep-RP Octyl (FO) contains monomerically bonded perfluorooctyl groups and is used for many pharmaceutical separations. The fluorinated octyl shows retention similar to that of standard C8 phases, but with unique selectivity. FluoroSep-RP Propyl (FP) is a short chain phase used for the separation of proteins, peptides and other related compounds. It is compatible with all standard buffers used in peptide work, including low TFA eluents. FluoroSep-RP Phase Column Dimensions (mm) Guard Cartridge (5/pk) 50 x x x x 4.6 (0 x.mm) μm Phenyl 5-FSP 5-FSP 5-FSP FSP Phenyl/HS 5-FSP/HS 5-FSP/HS 5-FSP/HS FSP/HS 5μm Phenyl 5-FSP 5-FSP 5-FSP 55-FSP 000-FSP Phenyl/HS 5-FSP/HS 5-FSP/HS 5-FSP/HS 55-FSP/HS 000-FSP/HS Octyl 5-FO 5-FO 5-FO 55-FO - Propyl 5-FP 5-FP 5-FP 55-FP - Use with guard cartridge holder 0000 ( 87) and column coupler 0006 ( 9) sales@hichrom.co.uk Tel:

4 GammaBond TM Alumina Spherical porous alumina core Stable from ph. to Unique selectivity GammaBond RP- used for USP method L9 GammaBond TM Alumina is a family of exceptionally stable alumina-based HPLC columns designed to provide high efficiency and unique selectivity for extreme ph applications. GammaBond Phase Particle Shape and Size Particle Size (μm) Pore Size (Å) ph Range Alumina Spherical alumina RP- Spherical alumina GammaBond Alumina is manufactured by bonding a polymer to a highly stable porous spherical 5μm alumina particle. This proprietary manufacturing process yields polymer-coated packings with the same high efficiency as traditional silica columns, but with all the advantages of alumina. The ES Industries GammaBond reversed-phase columns may be used with any eluent from ph. to ph, and any desired buffer system or additive. GammaBond RP- is a low load polybutadiene coated alumina, which exhibits both reversed-phase and ion-exchange characteristics, a useful combination in many separations. This phase is used for USP method L9. GammaBond Phase Column Length (mm) Internal Diameter (mm) Price Alumina 50 8-AL d8-al 58-AL 8 Alumina 00 8-AL d8-al 58-AL 57 Alumina 50 8-AL d8-al 58-AL 90 Alumina AL 5d8-AL 558-AL 47 Alumina RP ARP d7-arp 57-ARP 480 Alumina RP ARP d7-arp 57-ARP 597 Alumina RP ARP d7-arp 57-ARP 688 Alumina RP ARP 5d7-ARP 557-ARP 778 Chromegabond Chromegabond columns manufactured by ES Industries constitute a wide range of bonded phases, some being exclusive to ES Industries. Spherical porous silica supports are available in, 5 or 0μm particle sizes with pore diameters ranging from 60 to 000Å. A selection of the available phases is summarised below, with ordering information for 50 x 4.6mm i.d. columns. Please contact Hichrom for details of additional phases and for ordering information for alternative dimension columns. For free technical advice and support technical@hichrom.co.uk Chromegabond Phase (5μm) Chromegabond Phase (5μm) Code Description Catalogue No. Price Code Description Catalogue No. Price BAS-C8 Octadecyl 559-BAS-C8 64 CN-BD Diisopropyl Cyano 55-CN-BD 7 BAS-C8 Octyl 559-BAS-C8 64 CN-HS CN with higher surface area 55-CN/HS 7 BAS-CN Cyano 559-BAS-CN 64 C8-AI C8 with amide linkage 55-C8-AI 7 BAS-P Phenyl 559-BAS-P 64 D/RP Diol for RP 55-D/RP 7 HC-C8 Dense C8 55-HC-C8 64 A/RP Amino for RP 55-A/RP 7 TMS C 55-TMS 0 DA/RP Diamine for RP 55-DA/RP 7 C Dimethyl 55-C 0 TA/RP Triamine for RP 55-TA/RP 7 C4n-Butyl 55-C4 0 PSC C8/C8 55-PSC 64 C6 n-hexyl 55-C6 0 C C 55-C 64 MC-CC6 Cyclohexyl, endcapped 55-MC-CC6 4 LS Fluorinated C8 550-LS 64 C8 Octyl, non-endcapped 55-C8 0 LS/HS Fluorinated C8 55-LS/HS 64 C8 Octadecyl, non-endcapped 55-C8 0 ODS-PI Polar embedded C8 559-ODS-PI 64 MC8 Octadecyl, endcapped 55-MC8 0 NPI Monoalcohol for natural product isolation 55-NPI 4 WC8E Octadecyl 557-WC8E POA NO Nitro 55-NO 7 AP Alkyl Phenyl 55-AP 0 A/CN Mixed amino/cyano 55-A/CN 7 C8-BD Diisopropyl C8 55-C8-BD 7 RingSep For petroleum product applications 55-RS 595 C8-BD Diisopropyl C8 55-C8-BD 7 DNAP For petroleum product applications 55-DNAP 5 P-BD Diisopropyl Phenyl 55-P-BD 7 Silver/SI For petroleum product applications 55-AG/SI 7 00Å pore size other pore sizes available Ordering information is for 50 x 4.6mm i.d. columns.. Please enquire for details 0

5 ES Industries SFC Columns ES Industries offer a range of phases specifically designed for SFC separations. These GreenSep TM phases have proved to show superior separation, selectivity, peak shape and loading capacity compared to conventional normal-phase HPLC materials adapted for SFC (eg. amino, cyano, diol). The general features and ordering information for 50 x 4.6mm i.d. columns are highlighted below. All of these materials are also available in semi-preparative (0mm and 5mm i.d.) and preparative (0mm, 0mm and 50mm i.d.) dimensions. ES Industries also offer a range of Chromegabond SFC phases, including Amine HD, Cyano HS and Diol HD. Phase (5μm) Pore Size (Å) Features (50 x 4.6mm) Catalogue No. Price GreenSep TM Phase Ethylpyridine 0 Good for strongly basic compounds without use of additives 559-GS-PYE 9 Amino Phenyl 0 Normal phase mixed mode. Separation of amines, alcohols and acids without use of additives 559-GS-APH 9 Nitro 0 Useful for separation of geometrical isomers and diastereomers, also compounds containing aromatic groups, polarisable electrons and conjugate systems. Strong 559-GS-NO 9 charge transfer mechanism Pyridyl Amide 0 Separation of compounds functionalized with both amine bases and acidic groups. Does not require addition of TFA or amine modifier 559-GS-PYA 9 DEAP (Diethylaminopropyl) 0 Shows greater selectivity, superior peak shapes and increased loading capacity than conventional amino phases. Amine additives not required 559-GS-DEAP 9 PFP 0 Separation of geometrical isomers and diastereomers. Recommended for compounds containing aromatic groups, polarisable electrons and conjugate 559-GS-PFP 9 systems. Also suitable for halogenated compounds Basic 0 Based on imidazole chemistry. Good retention of amine-containing basic compounds 559-GS-BC 9 Silica 0 Ultra high purity silica specifically designed for SFC 559-GS-SI 9 Chromegabond Phase Amine HD 00 High loading for preparative applications 55-A/HD-SFC 57 Cyano HS 60 High surface area and high loading capacity 55-CN/HS-SFC 57 Diol HD 60 High density diol bonding for high loading capacity 55-D/HD-SFC POA GreenSep Guard Column System GreenSep GCS is a new guard column system developed specifically for use with preparative SFC columns. Hardware specially designed for SFC is employed and is packaged with a high performance coupler for attachment to the SFC column. GreenSep GCS is packed with highly deactivated, high performance silica particles and is designed to maintain the chromatographic integrity of the system. Four sizes are available, depending on the diameter of the preparative column. Please enquire with Hichrom for ordering details. Column: GreenSep Ethylpyridine (5µm, 50 x 4.6mm) Eluent: 5-50% Gradient CO :CH OH Flow rate:.5ml/min Temperature: 5 C -(4-Biphenyl)-6-phenylbenzoxazole Column: GreenSep Pyridyl Amide (5µm, 50 x 4.6mm) Eluent: 5-50% Gradient CO :CH OH Flow rate:.5ml/min Temperature: 5 C,5-Diethoxy-4-morpholinoaniline -( -Benzimidazolyl)-7- N,N-diethyl-aminocoumarin Furosemide Mins Mins Figure. SFC of basic compounds Figure 4. SFC of basic and acidic compounds sales@hichrom.co.uk Tel: