BioMater Centre. - the equipment and services at the university - Virpi Tiitu Translational research-kuh and UEF opportunities
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1 BioMater Centre - the equipment and services at the university - Virpi Tiitu Translational research-kuh and UEF opportunities
2 Basic Role of BioMater Centre "BioMater Centre acts as an independent department to provide services and equipment for researchers in different faculties." Operational focus areas in the Centre: to develop methods and modernize the equipment for biomaterials research to develop and activate education, instrumentation and research for the specific fields of research to utilize the equipment and know-how for the industrial needs One of the goals is to support development of education and research of technical and natural sciences
3 Status of BioMater in UEF Supports many of the strong research areas in UEF: Molecular medicine, drug development, biotechnology Environmental health and climate change Optics and new materials Forestry and environment BioMater acts as an interface to local high tech companies (analytical services, etc.) national companies and research centers and even to international companies and research networks in demanding projects
4 BioMater Centre in the University of Eastern Finland The goverment of the University of Eastern Finland Rector, academic rector Philosophical Faculty Faculty of Science and Forestry Faculty of Health Sciences Faculty of Social Sciences and Business studies BioMater - SMARC - InFotonics SIB-labs 2011
5 Laboratories in the BioMater Material testing: Centre Instron, Lloyd, hip joint simulator (destructive) Impedance, roughness, etc. (non-destructive) Microscopy: Optical microscopy (incl. confocal microscopy) Electron microscopy (TEM, SEM) Spectroscopy (FTIRi, FTIR, Raman) Sample preparation (EM samples, hard tissues) (Bio)chemistry Structural analysis: microct, ultrasound
6 Microscopy: Optical microscope 1 Nikon Microphot FXA Location S3151 Versatile optics Objectives: 1x - 100x Transmitted light; BF, DF Epifluorescence DIC (differential interference contrast microscopy), Phase contrast Color camera Examples: DIC (toluidine blue) Normal tr.light (epididymis) Fluorescence (pollen)
7 Microscopy: Optical microscope2 Zeiss AxioImager M2 Location S3136 Fully motorized (not yet in full operation) Objectives: 2.5x - 40x Transmitted light Polarization Color camera Examples: Bone (2 stains) Cartilage (2.5x) Plant root (40x)
8 Extra options: Zeiss AxioImager Extended focus - Each section in focus Topography using Reflected light
9 Microscopy: Confocal microscope Nikon TE-300 with UltraVIEW confocal unit Location S3151 Inverted microscope Objectives: 4x - 100x Confocal unit: Spinning disc 3-laser excitation 488 nm, 568 nm, 647 nm Z-stack, time-lapse imaging Examples: Pollen Cell culture (2 stains) Cartilage
10 Microscopy: Transmission electron microcope TEM is a method, where high energy electrons are used for (high resolution) imaging the specimen (structure) In vivo imaging Light microscopy Electronmicroscopy Resolution - Resolution +
11 JEM-2100F, Jeol Location S3144 Operation voltage up to 200 kv (possible also 80 kv) Magnification: 50x 1,5miljx
12 Examples: Bacteria: Golgi in a cell: Microvillae: Epithelial cells: Tails of fish sperm:
13 Modern TEM: Various applications Negative Staining Correlative Microscopy Immunogold labeling Cryo-TEM Tomography Elemental analysis
14 Pilot samples studied during autumn 2010 Tissues ImmunoAu labeled tissue Rat brain nanogold particles for labeling Artery in rat brain
15 Case-studies 1 The diabetes-prone NZO/HI stain (Pancreatic immunopathology) Electron micrographs of islet sections. A, Detail of an islet of a 52-week-old female. Fully granulated beta cells surround one pancreatic polypeptide cell (PP). B, Detail of an islet of an age-matched chronically diabetic male. Most beta cells are degranulated.
16 Case study 2: Viral nanoparticles as tools for intravital vascular imaging Intravital fluorescence imaging of chick CAM vasculature and subcellular localization of CPMV.
17 NANOTEM project 3-year projects to develope know-how and modernize equipment in the field of electron microscopy Organized by BioMater Centre Funding: State provincial office of Eastern Finland Also in the future: Tekes? Tekes-RDF Company-related development (2011 -)? Education: NANOTEM Lecture Series Practical training Instrumentation: Modern TEM Elemental analysis Tomography, etc. State provincial office-esf Educational project ( ) State provincial office-rdf Instrumentation ( )
18 Microscopy: Scanning electron microscope ESEM TMP XL30, Fei Company Location S3140 Operation voltage up to 30 kv Magnification 20x max x
19 Examples: ESEM TMP XL30 Pollen: Cultured cells: Bacteria: Red cells:
20 Microscopy: Sample preparation 1 EM sample preparation (Virpi Miettinen) Location S3137 and S3141 Samples for TEM and SEM need fixation dehydration embedding cutting staining fixation dehydration drying coating
21 Microscopy: Sample preparation2 Hard tissue laboratory (Ritva Sormunen) Location S3142 For demanding samples consisting metals and soft tissues, or large samples, for example dehydration - embedding - sawing with diamond saw - grinding - staining Examples: hip implant stent in a vein cadaver femoral bone
22 Microscopy: Spectroscopy "Imaging FTIR", PerkinElmer Spotlight Location S3145 Embedded or cryo-sections, special IR window needed "Raman spectrometer", Bruker Optics Senterra 200LX Location S3145 Tissue sections or blocks Optical image Spectral imaging Examples; collagen distribution in cartilage, typing of tumours, homogeneity of tablets, polymorphism
23 Structural Analysis The equipment include for example MicroCT, ultrasonic microscope Examples; - composition of bone, cartilage and tablets using ultrasonic microscope - structure of wood, bone and tablets using MicroCT
24 Contact information For more information, please visit Location: Snellmania Building 3 rd floor, corridor C
25 SIB-labs Three different units: Smarc-material research center (Joensuu), InFotonics Center (Joensuu) and Biomater centre (Kuopio) SIB-labs Research areas/interests: Material techniques Optics Biomaterials Focused Research services Customise research Equipment (maintenance and development) Participate research programs and networks
26 Quite big organization: over 200 persons SIB-labs started beginning of the year
27 Thank you Virpi Tiitu:
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Optical microscopy Theoretical background Galina Kubyshkina
Optical microscopy Theoretical background Galina Kubyshkina Elektromaterial Lendava d.d., Slovenia Crystalline materials presence of a unit (cell), which is periodically repeated in space regular structure
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NORDIC LIFE SCIEN THE LEADING LIFE SCIENCE BUSINESS MAGAZINE 169 SEK / 20 EUR / 25 USD // UMEÅ // KUOPIO // TROMSØ ANTIBIOTIC RESISTANCE SWEDEN S LIFE SCIENCE STRATEGY ILLUSTRATION AJAK ARCHITECTS ALL