WILD OAT CONTROL STRATEGIES IN CLEARFIELD SPRING TURNIP RAPE

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1 Trial Report 2011 Herbicides, fungicides and insecticides WILD OAT CONTROL STRATEGIES IN CLEARFIELD SPRING TURNIP RAPE Trial ID Crop, variety Location Exp. starting date Exp. completion date H Spring turnip rape, Aurea CL Jokioinen 24/05/11 17/10/11 Purpose of trial: To compare the efficacy of wild oat control strategies with Clamox alone and in successive use with Focus Ultra in Clearfield spring turnip rape. SUMMARY Growing of spring turnip rape according to CLEARFIELD Production System developed by BASF has been possible from year 2011 in Finland. An IMI tolerant Finnish spring turnip rape variety Aurea CL and the herbicide Clamox (metazachlor 375 g/l + imazamox 17.5 g/l) have been registered and are available for growers. Clamox has been shown effective on many important dicot weed species and selective on turnip rape Aurea CL in several field studies in Finland before. Clamox has also efficacy on some grass weeds, among others on wild oat, Avena fatua. It is however unclear if Clamox alone can control wild oat effectively enough in turnip rape Aurea CL. The efficacy of wild oat control strategies with Clamox alone and in successive use with Focus Ultra (cycloxydim 100 g/l) were tested for BASF Agro Nordic/Baltic in one field trial in spring turnip rape Aurea CL in Jokioinen in The field trial was carried out according to GEP standards and relevant EPPO guidelines at in Because it was difficult to find a trial location with a natural and even occurrence of wild oat, the weed was simulated with common oat, Avena sativa. A trial site in Jokioinen with a low weed pressure was chosen. On May 24 th 2011, after one harrowing, a small amount (45 seeds/m 2 ) of oat Aslak was sown on the soil surface with a plot fertilizing machine and was harrowed again across the sowing direction. Immediately after that 9.7 kg/ha of turnip rape Aurea CL was sown across the sowing direction of oat with a normal combine fertilizer/sowing machine. There were six treatments in the field trial, untreated included. Clamox was tested at 2.0 l/ha and 1.5 l/ha doses, and compared with treatments with successive use of Clamox and Focus Ultra at doses 1.5 l/ha l/ha and 1.5 l/ha l/ha, respectively. Butisan S (metazachlor 500 g/l) at 2.0 l/ha followed with Focus Ultra at 1.5 l/ha was used as a reference treatment. The additive Dash at 1.0 l/ha was used in all applications with Clamox and at 0.5 l/ha in all applications with Focus Ultra. Clamox and Butisan S were sprayed on June 16 th 2011 (application time A), when turnip rape was at BBCH and oat at BBCH Focus Ultra was sprayed on June 21 st 2011 (application time B), when turnip rape was at BBCH and oat at BBCH The efficacy of treatments on oat was assessed visually 14 DA-A and 15 DA-B, and by taking plant samples on sample areas of one square metre per plot 22 DA-B. Also other weeds were sampled at the same time, and the number and the air-dry weight of each species were recorded. The phytotoxicity of treatments on turnip rape was assessed visually 7 and 13 DA-A and 15 DA-B. The yield of turnip rape was harvested on September 17 th 2011 (88, and the yield amount kg/ha, the purity % and the 1000 seed weight of the yield were recorded. Turnip rape and oat grew very evenly throughout the trial. In the untreated plots on average the number of oat (AVESA) plants was 30 per square metre on July 13 th at the weed sampling (27 DA-A, 22. Clamox was very effective on oat (Pictures 1-3, Figures 1-2). Already 14 DA-A the efficacy of the treatments with Clamox alone was % on oat, 15 DA-B 97 % and 22 DA-B %. All treatments with Focus Ultra destroyed oat finally 100 %, but statistically and practically there were no differences in the efficacy on oat between the herbicide treatments. There were also some dicot weeds in the trial, the most abundant species being Chenopodium album (CHEAL; 23 plants per square metre in the untreated). CHEAL was totally controlled in all treatments with Clamox (Figure 4). In the reference treatment with Butisan S the number of CHEAL was reduced only 10 % and the dry weight 59 %. Also Galeopsis spp. (GAESS) and Fumaria officinalis (FUMOF) were controlled more effectively with Clamox than with Butisan S. (Figures 5 and 6). The yield of turnip rape was rather low in the trial, only 1044 kg/ha in the untreated treatment (Figure 7). All herbicide treatments increased the yield statistically significantly, from 18 to 30 %. In the yield of the untreated treatment there were

2 Trial Report 2011 Herbicides, fungicides and insecticides a lot of oat seeds, and the purity of the yield was only 70.6 % (Figure 8). In the herbicide treatments there were practically no oat seeds in the yield, and the yield purities were from 87.7 % to 93.4 %. The herbicide treatments seemed to increase also the 1000 seed weight of turnip rape slightly, 6-8 % (Figure 9). The trial as a whole indicates that if wild oat is equally susceptible to Clamox as common oat, Clamox alone may be effective enough for the control of wild oat in Aurea CL turnip rape. Picture 1. The field trial H on August 2 nd An untreated plot in the foreground of the picture. Picture 2. Oat and Chenopodium album grew through the crop on August 2 nd 2011 on an untreated plot. Picture 3. A plot treated with Clamox at 2.0 l/ha (application A; treatment 2) was weed-free on August 2 nd 2011.

3 Trial Report 2011 Herbicides, fungicides and insecticides WILD OAT CONTROL STRATEGIES IN CLEARFIELD SPRING TURNIP RAPE, Jokioinen TRIAL H ;Focus Ultra 1.5 B;Dash 0.5 B ;Focus Ultra 1.0 B;Dash 0.5 B CONTROL % AVESA 14 DA-A CONTROL % AVESA 15 DA-B Figure 1. Visually assessed control (efficacy) % of treatments on oats (AVESA) 14 days after the herbicide application A (14 DA-A) on June 30 th and 15 DA-B on July 6 th 2011 ;Focus Ultra 1.5 B;Dash 0.5 B ;Focus Ultra 1.0 B;Dash 0.5 B AVESA no/m2 ( AVESA g/m2 (22 Figure 2. Number and air-dry weight of oats plants per square metre 22 DA-B on July 13 th 2011 ;Focus Ultra 1.5 B;Dash 0.5 B ;Focus Ultra 1.0 B;Dash 0.5 B TTTDD no/m2 ( TTTDD g/m2 (22 Figure 3. Number and air-dry weight of dicot weeds in total per square metre 22 DA-B

4 Trial Report 2011 Herbicides, fungicides and insecticides WILD OAT CONTROL STRATEGIES IN CLEARFIELD SPRING TURNIP RAPE, Jokioinen TRIAL H ;Focus Ultra 1.5 B;Dash 0.5 B ;Focus Ultra 1.0 B;Dash 0.5 B CHEAL no/m2 ( CHEAL g/m2 (22 Figure 4. Number and air-dry weight of Chenopodium album per square metre 22 DA-B ;Focus Ultra 1.5 B;Dash 0.5 B ;Focus Ultra 1.0 B;Dash 0.5 B GAESS no/m2 ( GAESS g/m2 (22 Figure 5. Number and air-dry weight of Galeopsis spp. per square metre 22 DA-B ;Focus Ultra 1.5 B;Dash 0.5 B ;Focus Ultra 1.0 B;Dash 0.5 B FUMOF no/m2 ( FUMOF g/m2 (22 Figure 6. Number and air-dry weight of Fumaria officinalis per square metre 22 DA-B

5 Trial Report 2011 Herbicides, fungicides and insecticides WILD OAT CONTROL STRATEGIES IN CLEARFIELD SPRING TURNIP RAPE, Jokioinen TRIAL H ;Focus Ultra 1.5 B;Dash 0.5 B ;Focus Ultra 1.0 B;Dash 0.5 B Yield of turnip rape kg/ha Pure yield of turnip rape kg/ha Figure 7. Total yield and pure yield (weed seeds and other trash removed) of turnip rape kg/ha 88 DA-B on September 17 th 2011 ;Focus Ultra 1.5 B;Dash 0.5 B ;Focus Ultra 1.0 B;Dash 0.5 B Figure ; Yield purity % Purity % of turnip rape seed yield

6 01/12/11 (H ) Confidential Trial Treatments Page 1 of 1 Trt Treatment Form Form Lot Rate Appl No. Name Conc Type Code Rate Unit Code 1 2 Clamox SC L/HA A -metazachlor g AI -imazamox g AI Dash EC L/HA A 3 Clamox SC L/HA A -metazachlor g AI -imazamox g AI Dash EC L/HA A 4 Clamox SC L/HA A -metazachlor g AI -imazamox g AI Dash EC L/HA A Focus Ultra 100 EC WO 1.5 L/HA B -cycloxydim g AI Dash EC L/HA B 5 Clamox SC L/HA A -metazachlor g AI -imazamox g AI Dash EC L/HA A Focus Ultra 100 EC WO 1.0 L/HA B -cycloxydim g AI Dash EC L/HA B 6 Butisan S 500 SC FRE L/HA A -metazachlor g AI Focus Ultra 100 EC WO 1.5 L/HA B -cycloxydim g AI Dash EC L/HA B Replications: 4, Conduct under GLP/GEP: Yes (GEP with audit trail), Design: Randomised Complete Block, Treatment units: Treated plot size, Dry Form. Unit: g/kg, Treated plot size Width: 3 meters, Treated plot size Length: 8 meters, Application volume: 200 l/ha, Mix size: 3 litres, Format definitions: G-All7.DEF, G-All7.FRM

7 09/12/11 (H ) Confidential Site Description Page 1 of 4 General Trial Information Title: Research Scientist Affiliation: Postal Code: FI pentti.ruuttunen@mtt.fi Trial Location City: Jokioinen Trial Status: ONE-YEAR/FINAL Postal Code: FI Initiation Date: 10/06/11 Country: FIN Planned Completion Date: 30/11/11 N -Latitude of LL Corner : E -Longitude of LL Corner : Map Reference: WGS 84 Conducted Under GEP: X Guideline Description 1. PP 1/181(3) Conduct and reporting of efficacy evaluation trials including GEP 2. PP 1/152(3) Design and analysis of efficacy evaluation trials 3. PP 1/49(3) Weeds in brassica oil crops Objectives: Evaluation of wild oat control strategies in Clearfield spring turnip rape. Wild oat was simulated with common oat, Avena sativa, which was drilled at the same time with turnip rape. Conclusions: Turnip rape and oat grew very evenly throughout the trial. In the untreated plots on average the number of oat (AVESA) plants was 30 per square metre on July 13 th at the weed sampling (27 DA-A, 22. Clamox was very effective on oat. Already 14 DA-A the efficacy of the treatments with Clamox alone was % on oat, 15 DA-B 97 % and 22 DA-B %. All treatments with Focus Ultra destroyed oat finally 100 %, but statistically and practically there were no differences in the efficacy on oat between the herbicide treatments. There were also some dicot weeds in the trial, the most abundant species being Chenopodium album (CHEAL; 23 plants per square metre in the untreated). CHEAL was totally controlled in all treatments with Clamox. In the reference treatment with Butisan S the number of CHEAL was reduced only 10 % and the dry weight 59 %. Also Galeopsis spp. (GAESS) and Fumaria officinalis (FUMOF) were controlled more effectively with Clamox than with Butisan S. The yield of turnip rape was rather low in the trial, only 1044 kg/ha in the untreated treatment. All herbicide treatments increased the yield statistically significantly, from 18 to 30 %. In the yield of the untreated treatment there were a lot of oat seeds, and the purity of the yield was only 70.6 %. In the herbicide treatments there were practically no oat seeds in the yield, and the yield purities were from 87.7 % to 93.4 %. The herbicide treatments seemed to increase also the 1000 seed weight of turnip rape slightly, 6-8 %. The trial as a whole indicates that if wild oat is equally susceptible to Clamox as common oat, Clamox alone may be effective enough for the control of wild oat in Aurea CL turnip rape. Crop Description Crop 1: BRSSP Brassica rapa sylvestris praec Spring turnip rape Variety: Aurea CL Description: IMI tolerant Finnish variety BBCH Scale: BRAP Planting Date: 24/05/11 Planting Method: SEEDED Rate, Unit: 9.7 KG/HA Row Spacing, Unit: 12.5 CM Harvest Date: 17/09/11 Harvest Equipment: Plot combiner Sampo Harvested Width, Unit: 2 M Harvested Length, Unit: 7.25 M

8 09/12/11 (H ) Confidential Site Description Page 2 of 4 Crop 2: AVESA Avena sativa Oat, common Variety: Aslak BBCH Scale: BCER Planting Date: 24/05/11 Planting Method: SEEDED Rate, Unit: 45 S/M2 Pest Description Pest 1 Type: W Code: AVESA Avena sativa Common Name: Common oat Pest 2 Type: W Code: CHEAL Chenopodium album Common Name: Lambsquarters, common Pest 3 Type: W Code: GAESS Galeopsis sp. Common Name: Hempnettle Pest 4 Type: W Code: VIOAR Viola arvensis Common Name: Field violet Pest 5 Type: W Code: FUMOF Fumaria officinalis Common Name: Fumitory, common Site and Design Plot Width, Unit: 3 M Site Type: FIELD Plot Length, Unit: 8 M Tillage Type: CONVENTIONAL-TILL Replications: 4 Study Design: Randomized Complete Block Trial Initiation Comments: After one harrowing Aslak oat was sown on the soil surface with a plot fertilizing machine and the soil was harrowed again across the sowing direction of oat. Immediately after that Aurea CL turnip rape was sown across the sowing direction of oat with a normal combine fertilizer/sowing machine. Maintenance Maintenance Rate No. Date Treatment Name Rate Unit 1. 24/05/11 Pellon Y1 (N:27 P:2 K:2) 330 KG/HA 2. 02/06/11 Mavrik 0.2 L/HA 3. 08/06/11 Decis 0.2 L/HA 4. 10/06/11 Decis 0.2 L/HA 5. 17/06/11 Decis 0.2 L/HA 6. 23/06/11 Karate 2.5 WG 0.3 KG/HA Soil Description % OM: 4.53 Texture: CLAY LOAM ph: 6.4

9 09/12/11 (H ) Confidential Site Description Page 3 of 4 Additional Measured Elements Element Quantity Unit Ca 2844 MG/L P MG/L K MG/L Mg MG/L Moisture and Weather Conditions Overall Moisture Conditions: See the appendix of weather data Closest Weather Station: Jokioinen Distance: 3.13 Unit: KM Application Description A B Application Date: 16/06/11 21/06/11 Time of Day: 14:40 7:45 Application Method: SPRAY SPRAY Application Placement: BROFOL BROFOL Applied By: PR,LR,EMT PR,LR,JA Air Temperature, Unit: 21.0 C 11.1 C % Relative Humidity: Wind Velocity, Unit: 1.3 M/S 1.3 M/S Dew Presence (Y/N): N Y Soil Moisture: DAMP DAMP % Cloud Cover: Next Rain Occurred On: 21/06/11 Crop Stage At Each Application A B Crop 1 Code, BBCH Scale: BRSSP BRAP BRSSP BRAP Stage Scale Used: BBCH BBCH Stage Majority, Percent: Height, Unit: 2 CM 6 CM Height Minimum, Maximum:

10 09/12/11 (H ) Confidential Site Description Page 4 of 4 Pest Stage At Each Application A B Pest 1 Code, Disc., Scale: AVESA W BBCH AVESA W BBCH Stage Majority, Percent: Height, Unit: 9 CM 14 CM Height Minimum, Maximum: Pest 2 Code, Disc., Scale: CHEAL W BBCH CHEAL W BBCH Stage Majority, Percent: Height, Unit: 3 CM 4 CM Height Minimum, Maximum: Pest 3 Code, Disc., Scale: GAESS W BBCH GAESS W BBCH Stage Majority, Percent: Height, Unit: 6 CM 10 CM Height Minimum, Maximum: Pest 4 Code, Disc., Scale: VIOAR W BBCH VIOAR W BBCH Stage Majority, Percent: Height, Unit: 0.8 CM 0.8 CM Height Minimum, Maximum: Pest 5 Code, Disc., Scale: FUMOF W BBCH FUMOF W BBCH Stage Majority, Percent: Height, Unit: 3 CM 4 CM Height Minimum, Maximum: Application Equipment A B Appl. Equipment: PLOT SPRAYER PLOT SPRAYER Operating Pressure, Unit: 1.9 BAR 1.9 BAR Nozzle Type: HARDI4110 HARDI4110 Nozzle Size: Nozzle Spacing, Unit: 50 CM 50 CM Boom ID: KSU 4 KSU 4 Boom Length, Unit: 3 M 3 M Boom Height, Unit: 50 CM 50 CM Ground Speed, Unit: 1.0 M/S 1.0 M/S Spray Volume, Unit: 200 L/HA 200 L/HA Mix Size, Unit: 3 Liters 3 Liters Propellant: AIR AIR Date By Notes 21/06/11 PR Some rain in the afternoon (1.5 mm at Jokioinen observatory on that day)

11 01/12/11 (H ) Confidential AOV Means Table Page 1 of 3 Pest Code AVESA AVESA AVESA Crop Code BRSSP BRSSP BRSSP BRSSP BRSSP Part Rated PLANT PLANT PLANT PLANT PLANT PLANT PLANT PLANT Rating Date 16/06/11 16/06/11 16/06/11 16/06/11 23/06/11 29/06/11 30/06/11 06/07/11 Rating Data Type COVER COVER COUPLA COUPLA PHYGEN PHYGEN CONTRO PHYGEN Rating Unit % % NUMBER NUMBER % % % % Sample Size Sample Size Unit M2 M2 M2 M2 M2 M2 M2 M2 Crop Stage Crop Stage Scale BBCH BBCH BBCH BBCH BBCH BBCH BBCH BBCH Pest Stage Assessed By PR PR PR PR PR PR PR PR Days After First/Last Applic Trt-Eval Interval 0 DA-A 0 DA-A 0 DA-A 0 DA-A 7 DA-A 13 DA-A 14 DA-A 15 DA-B Plant-Eval Interval 23 DP-1 23 DP-1 23 DP-1 23 DP-1 30 DP-1 36 DP-1 37 DP-1 43 DP-1 ARM Action Codes P P TST[3] TST[5] P P P P Trt Treatment Rate Appl No. Name Rate Unit Code Clamox 2.0 L/HA A Dash 1.0 L/HA A 3 Clamox 1.5 L/HA A Dash 1.0 L/HA A 4 Clamox 1.5 L/HA A Dash 1.0 L/HA A Focus Ultra 1.5 L/HA B 5 Clamox 1.5 L/HA A Dash 1.0 L/HA A Focus Ultra 1.0 L/HA B 6 Butisan S 2.0 L/HA A Focus Ultra 1.5 L/HA B Column 3: TST[3] = Standardise[3] Column 4: TST[5] = Standardise[5]

12 01/12/11 (H ) Confidential AOV Means Table Page 2 of 3 Pest Code AVESA CHEAL GAESS FUMOF VIOAR XXXDD TTTDD AVESA Crop Code Part Rated PLANT PLACUT PLACUT PLACUT PLACUT PLACUT PLACUT PLACUT Rating Date 06/07/11 13/07/11 13/07/11 13/07/11 13/07/11 13/07/11 13/07/11 13/07/11 Rating Data Type CONTRO COUPLA COUPLA COUPLA COUPLA COUPLA COUPLA COUPLA Rating Unit % NUMBER NUMBER NUMBER NUMBER NUMBER NUMBER NUMBER Sample Size Sample Size Unit M2 M2 M2 M2 M2 M2 M2 M2 Crop Stage Crop Stage Scale BBCH Pest Stage Assessed By PR LR LR LR LR LR LR LR Days After First/Last Applic Trt-Eval Interval 15 DA-B 22 DA-B 22 DA-B 22 DA-B 22 DA-B 22 DA-B 22 DA-B 22 DA-B Plant-Eval Interval 43 DP-1 50 DP-1 50 DP-1 50 DP-1 50 DP-1 50 DP-1 50 DP-1 50 DP-1 ARM Action Codes P APOC APOC APOC APOC APOC T4 APOC APOC Trt Treatment Rate Appl No. Name Rate Unit Code (100%) (100%) (100%) (100%) (100%) (100%) (100%) 2 Clamox 2.0 L/HA A Dash 1.0 L/HA A (1%) (0%) (22%) (44%) (0%) (9%) (5%) 3 Clamox 1.5 L/HA A Dash 1.0 L/HA A (0%) (25%) (6%) (28%) (11%) (7%) (1%) 4 Clamox 1.5 L/HA A Dash 1.0 L/HA A (0%) (6%) (0%) (72%) (11%) (10%) (0%) Focus Ultra 1.5 L/HA B 5 Clamox 1.5 L/HA A Dash 1.0 L/HA A (1%) (6%) (6%) (39%) (0%) (7%) (0%) Focus Ultra 1.0 L/HA B 6 Butisan S 2.0 L/HA A Focus Ultra 1.5 L/HA B (90%) (50%) (72%) (56%) (100%) (80%) (0%) Column 15: T4

13 01/12/11 (H ) Confidential AOV Means Table Page 3 of 3 Pest Code CHEAL GAESS FUMOF VIOAR XXXDD TTTDD AVESA Crop Code Part Rated PLACUT PLACUT PLACUT PLACUT PLACUT PLACUT PLACUT Rating Date 13/07/11 13/07/11 13/07/11 13/07/11 13/07/11 13/07/11 13/07/11 Rating Data Type WEIDRY WEIDRY WEIDRY WEIDRY WEIDRY WEIDRY WEIDRY Rating Unit G G G G G G G Sample Size Sample Size Unit M2 M2 M2 M2 M2 M2 M2 Crop Stage Crop Stage Scale Pest Stage Assessed By LR LR LR LR LR LR LR Days After First/Last Applic Trt-Eval Interval 22 DA-B 22 DA-B 22 DA-B 22 DA-B 22 DA-B 22 DA-B 22 DA-B Plant-Eval Interval 50 DP-1 50 DP-1 50 DP-1 50 DP-1 50 DP-1 50 DP-1 50 DP-1 ARM Action Codes APOC APOC APOC APOC APOC T5 APOC APOC Trt Treatment Rate Appl No. Name Rate Unit Code (100%) (100%) (100%) (100%) (100%) (100%) (100%) 2 Clamox 2.0 L/HA A Dash 1.0 L/HA A (0%) (0%) (1%) (100%) (0%) (1%) (0%) 3 Clamox 1.5 L/HA A Dash 1.0 L/HA A (0%) (18%) (0%) (0%) (0%) (2%) (0%) 4 Clamox 1.5 L/HA A Dash 1.0 L/HA A (0%) (2%) (0%) (8%) (0%) (0%) (0%) Focus Ultra 1.5 L/HA B 5 Clamox 1.5 L/HA A Dash 1.0 L/HA A (0%) (0%) (0%) (0%) (0%) (0%) (0%) Focus Ultra 1.0 L/HA B 6 Butisan S 2.0 L/HA A Focus Ultra 1.5 L/HA B (41%) (187%) (39%) (54%) (59%) (53%) (0%) Column 22: T5

14 02/12/11 (H ) Confidential AOV Means Table Page 1 of 2 Pest Code TTTDD TTTDD AVESA AVESA TTTDD AVESA AVESA Crop Code BRSSP Part Rated PLACUT PLACUT PLACUT PLACUT PLACUT PLACUT PLACUT SEED Rating Date 13/07/11 13/07/11 13/07/11 13/07/11 13/07/11 13/07/11 13/07/11 17/09/11 Rating Data Type COUPLA COUPLA COUPLA COUPLA WEIDRY WEIDRY WEIDRY YIELD Rating Unit NUMBER NUMBER NUMBER NUMBER G G G KG Sample Size Sample Size Unit M2 M2 M2 M2 M2 M2 M2 HA Crop Stage 99 Crop Stage Scale BBCH Assessed By LR LR LR LR LR LR LR AM Days After First/Last Applic Trt-Eval Interval 22 DA-B 22 DA-B 22 DA-B 22 DA-B 22 DA-B 22 DA-B 22 DA-B 88 DA-B Plant-Eval Interval 50 DP-1 50 DP-1 50 DP-1 50 DP-1 50 DP-1 50 DP-1 50 DP DP-1 ARM Action Codes T4 APOC TS[17] APOC TL[18] T5 APOC APOC TL[25] TY3 APOC Trt Treatment Rate Appl No. Name Rate Unit Code a 6.15 a 30 a 1.46 a 38.1 a 84.5 a 1.92 a 1044 b (100%) (100%) (100%) (100%) (100%) 2 Clamox 2.0 L/HA A 3 b 1.77 b 2 b 0.30 b 0.4 b 0.1 b 0.02 b 1273 a Dash 1.0 L/HA A (9%) (5%) (1%) (0%) (122%) 3 Clamox 1.5 L/HA A 3 b 1.68 b 0 b 0.08 b 0.6 b 0.0 b 0.00 b 1283 a Dash 1.0 L/HA A (7%) (1%) (2%) (0%) (123%) 4 Clamox 1.5 L/HA A 4 b 1.76 b 0 b 0.00 b 0.1 b 0.0 b 0.00 b 1273 a Dash 1.0 L/HA A (10%) (0%) (0%) (0%) (122%) Focus Ultra 1.5 L/HA B 5 Clamox 1.5 L/HA A 3 b 1.65 b 0 b 0.00 b 0.0 b 0.0 b 0.00 b 1356 a Dash 1.0 L/HA A (7%) (0%) (0%) (0%) (130%) Focus Ultra 1.0 L/HA B 6 Butisan S 2.0 L/HA A 31 a 5.53 a 0 b 0.00 b 20.3 b 0.0 b 0.00 b 1233 a Focus Ultra 1.5 L/HA B (80%) (0%) (53%) (0%) (118%) LSD (P=.05) Standard Deviation CV Bartlett's X P(Bartlett's X2) 0.042* * * 0.001* Replicate F Replicate Prob(F) Treatment F Treatment Prob(F) Means followed by same letter do not significantly differ (P=.05, Student-Newman-Keuls) Column 1: T4 Column 2: TS[17] = SQR([17] +.5) Column 4: TL[18] = LOG([18]+ 1) Column 5: T5 Column 7: TL[25] = LOG([25]+ 1) Column 8: TY3 = *[C26]*(100-[C36])/91

15 02/12/11 (H ) Confidential AOV Means Table Page 2 of 2 Pest Code Crop Code BRSSP BRSSP BRSSP Part Rated SEED SEED SEED Rating Date 29/09/11 17/09/11 17/10/11 Rating Data Type PURITY PUREYIELD 1000WEIG Rating Unit % KG G Sample Size Sample Size Unit G HA SEED Crop Stage Crop Stage Scale BBCH BBCH BBCH Assessed By EMT AM LR Days After First/Last Applic Trt-Eval Interval 100 DA-B 88 DA-B 88 DA-B Plant-Eval Interval 128 DP DP DP-1 ARM Action Codes T2 T6 APOC T1 APOC Trt Treatment Rate Appl No. Name Rate Unit Code b 730 b 3.30 a (100%) (100%) 2 Clamox 2.0 L/HA A 92.1 a 1172 a 3.56 a Dash 1.0 L/HA A (161%) (108%) 3 Clamox 1.5 L/HA A 93.0 a 1193 a 3.53 a Dash 1.0 L/HA A (164%) (107%) 4 Clamox 1.5 L/HA A 87.7 a 1123 a 3.57 a Dash 1.0 L/HA A (154%) (108%) Focus Ultra 1.5 L/HA B 5 Clamox 1.5 L/HA A 93.4 a 1266 a 3.56 a Dash 1.0 L/HA A (174%) (108%) Focus Ultra 1.0 L/HA B 6 Butisan S 2.0 L/HA A 90.7 a 1118 a 3.49 a Focus Ultra 1.5 L/HA B (153%) (106%) LSD (P=.05) Standard Deviation CV Bartlett's X P(Bartlett's X2) 0.004* 0.035* Replicate F Replicate Prob(F) Treatment F Treatment Prob(F) Means followed by same letter do not significantly differ (P=.05, Student-Newman-Keuls) Column 9: T2 = 100*[C29]/[C28] Column 10: T6 = [C27]*[C30]/100 Column 11: T1 = 10*((100-[C36])*[C33])/91

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