Laboratoire Arago Observatoire Océanologique Banyuls-sur-mer
|
|
|
- Stephen Atkins
- 6 years ago
- Views:
Transcription
1 Laboratoire Arago Observatoire Océanologique Banyuls-sur-mer University Pierre & Marie Curie (Paris 6) Centre National de la Recherche Scientifique Institut National des Sciences de l Univers et de l Environnement
2 Laboratoire d Océanologie Biologique (LOB) Banyuls-sur-mer Conference Room seats
3 Teaching in Banyuls Between 800 and students per year Teaching and lessons outside
4 Station vessel coastal area 6-8 persons
5 Monitoring stations Station SOLA : 26m depth Weekly sampling : -T C, S, Fluo - Nutrients - Chla - Bacterial counts, SSCP Station MOLA : 1000m depth At least monthly sampling. Additional samples based on real-time monitoring of basic Parameters (T C, S, Fluo) - Nutrients - Chla, phyto (FCM) - Bacterial counts, SSCP, DNA
6 2 research units UMR 7621, Director : A. Grémare Laboratoire d océanographie biologique de Banyuls, 2 teams «Processus and biological production at the interface watersediment» and «Diversity and functioning of pelagic ecosystems» UMR 7628, Director : G. Bœuf Models in cellular and evolutive biology, 2 teams «Cellular and integrated biology» and «Dispersion strategies and consequencies on the structuration of species and evolution»
7 Laboratory of Biological Oceanography (LOB) Team 1 : Processus and biological production at the interface water-sediment Team 2 : Diversity and functioning of pelagical ecosystems Microbial ecology group involved in MARPLAN
8 Microbial Ecology Group Head of the team : Philippe Lebaron Philippe Lebaron Biodiversity and functions of bacteria in the oceans Ingrid Obernosterer Autotrophy vs heterotrophy Role of the bacterioneuston in the carbon cycle Julia Baudart Water quality - pathogens Fabien Joux Effect of UV radiations on bacterial communities Jean-François Ghiglione Biodegradation of Pollutants - microarrays Muriel Bourrain Biodiversity and biomolecules
9 Linking diversity and functions 1 - Diversity : 4 approaches - Fingerprinting methods : SSCP - Cloning/sequencing/phylogeny/probes - Fluorescent in situ hybridization - Isolation of strains, characterization, collection and database
10 Time scale variability : 4 Months (March to June 2002) PCR 16S rdna / CE-SSCP analysis March April Same OTU in the 4 profiles : 15 / 29 (1,3,4,5,9,10,11,12,13,15,17,18,19,23, May June Ghiglione et al, AME 2005 (in press)
11 Cloning / sequencing phylogeny Phylogenetic relationships between AlphaProteobacteria Banyuls clones and relative genbank sequences T31 47 T31 5 T CloneCHAB-I-5 AJ2409 CloneKTc0993 AF23512 T31 96 T31 15 T T31 6 CloneAS-26 AJ T31 77 T T T Strain GMD29F12.1 AY StrainATAM AF3 CloneLA1-B32N AF5139 T T31 14 CloneNAC11-3 AF24563 CloneDC AY145 T31 98 T Octadecabacter sp. A T31 36 ClonePLY-P3-48 AY354 T31 37 CloneNAC11-7 AF24563 T31 24 T31 88 T T31 97 Sphingomonas sp. AF4 T T31 63 CloneZD0209 AJ CloneOCS126 AF T31 4 CloneOCS12 U75252 T Pelagibacter ubique ClonePLY43 U13159 clonearctic95a-16 AF CloneMB11E07 AY03330 T31 1 T T T31 87 CloneMB11F01 AY03330 CloneEBAC40E09 AF268 T31 7 T31 83 CloneZD0410 AJ T31 71 Roseobacter (15.2% of the library) SAR116 (1.6% of the library) SAR11 (4.9% of the library) 10% divergence
12 Bacterial 16S Ribosomal RNA Fluorescence In-Situ Hybridization (FISH) Probe AGACUGCUAACACAGGC + fluorescent dye (CY3, FITC,..)
13 Fluorescent in situ hybridization (FISH and CardFish) QuickTime et un décompresseur TIFF (non compressé) sont requis pour visionner cette image.
14 Isolation - Solid medium (R2A, Zobell, ) - Dilution technique - Extinction technique - Different enrichment conditions Purification of isolates Metabolic characterization Collection -80 C -DMSO - Glycerol - Cryobeads SSCP profile Sequencing - identification Bioactivity Screening on different targets UV resistance FCM signature under standardized conditions Biovolume, carbon and nitrogen content,..
15 Solar simulator *
16 Linking diversity and functions - Cell sorting using FCM - MicroFish - Metaproteomic : new in the lab
17 1 - Cell sorting by FCM Prelabeling Radioactive substrates Nucleic acid probes Physiological probes Laser (488 nm) Trash Further analyses of sorted fractions * Activity (radioactivity) * Identification * Isolation * Chemical analyses (C; N; P,.)
18 Micro-FISH Radioactive compounds ( 3 H, 14 C, 35 S) amino acids, leucine, glucose, DMSP, polysaccharides, protein Detect silver grains around assimilating cells Combine with phylogenetic probes to identify which groups assimilate the specific compound
19 DAPI stain + silver grains Probe positive From Dave Kirchman and Matt Cottrell
20 An original and well equiped cytometry plate-form in Banyuls : - Transmission electronic microscope (TEM) - Confocal laser microscope (CLM) - 2 Flow cytometers equipped with cell sorters (FCM) - Epifluorescence microscope and image analysis system - Solid-phase cytometer (SPC) Involved in the development of new cytometers
21 - Solid-phase cytometry : a new concept in cytometry with great potential in populational ecology - Flow cytometry cell sorting : an interesting approach for linking diversity and functions
22 Cytometry laboratory FACSCalibur FACSVantage High speed cell sorter
23 Solid-phase cytometry : a new technique for populational ecology - Detection and quantification of specific populations of bacteria, phytoplankton and zooplankton - High resolution : detection of rare events
24 Oscillating mirrors (scan < 3 mn) Oscillating mirrors Laser Line-to-line space = 3 µm Laser spot = 7 µm Recording each 0.5 µm Detectors
25 Solid phase cytometer (ChemScan RDI)
26 LOB interest in MARPLAN Single cell analysis : FISH / MICROFISH : training, improve protocols, inter-comparison of results between different groups, Cell sorting techniques : training, development of new protocols to improve PCR amplification of sorted cells, to share protocols between groups Collection of isolates (bacteria,..more????) : Environmental collections and databases : can we share our data and organize a single database? At least, we could develop a website on which collections and databases could be linked. A listing of strains available in these collections will be essential for the upcoming genomic program. Can we share expertises for the characterization of environmental strains? Training : We can organize in Banyuls (2007) : A workshop and/or a practical course on FISH in collaboration with Roscoff (Banyuls : bacteria / Roscoff : phytoplankton) - intercomparison of methods
Microbial Diversity and Assessment (III) Spring, 2007 Guangyi Wang, Ph.D. POST103B
Microbial Diversity and Assessment (III) Spring, 2007 Guangyi Wang, Ph.D. POST103B guangyi@hawaii.edu http://www.soest.hawaii.edu/marinefungi/ocn403webpage.htm Overview of Last Lecture Taxonomy (three
How to quantify bacteria in sediments?
How to quantify bacteria in sediments? Parkes, R.J., B.A. Cragg and P. Wellsbury, 2000 Phasecontrast microscopy Counting chamber (Thoma, Petroff-Hausser,...) Only feasable for liquid samples. Perry & Staley,
Chapter 10: Classification of Microorganisms
Chapter 10: Classification of Microorganisms 1. The Taxonomic Hierarchy 2. Methods of Identification 1. The Taxonomic Hierarchy Phylogenetic Tree of the 3 Domains Taxonomic Hierarchy 8 successive taxa
Insight into microbial world molecular biology research in environmental microbiology
Insight into microbial world molecular biology research in environmental microbiology Aleksandra Ziembi ska The Silesian University of Technology, Environmental Biotechnology Department aleksandra.ziembinska@polsl.pl
REPORT ON THE RESEARCH UNIT:
Research evaluation REPORT ON THE RESEARCH UNIT: Laboratory of Microbial Biodiversity and Biotechnology (LBBM) UNDER THE SUPERVISION OF THE FOLLOWING INSTITUTIONS AND RESEARCH BODIES: Université Pierre
Recent technology allow production of microarrays composed of 70-mers (essentially a hybrid of the two techniques)
Microarrays and Transcript Profiling Gene expression patterns are traditionally studied using Northern blots (DNA-RNA hybridization assays). This approach involves separation of total or polya + RNA on
Index. Index 419. B Bacillus mojavensis, Bacterial cells, 14, 17, 21, 103, 104, 147, 182, 183, 214, 354, 360, 370, 398 isolation, 14, 370
Index 419 Index 13 C NMR, in vivo, 173 175, 183 186, 396, 402 16S rdna, 3 8, 25, 30, 31, 56 58, 159, 160, 235, 412 amplification, 23 27, 62, 78 81, 237 240, 325, 407, 408, 411 library construction, 78
Does the High Nucleic Acid Content of Individual Bacterial Cells Allow Us To Discriminate between Active Cells and Inactive Cells in Aquatic Systems?
Does the High Nucleic Acid Content of Individual Bacterial Cells Allow Us To Discriminate between Active Cells and Inactive Cells in Aquatic Systems? Philippe Lebaron, Pierre Servais, Helene Agogué, Claude
Molecular Evolution and Ecology. Martin Polz
Molecular Evolution and Ecology Martin Polz mpolz@mit.edu Overview I. Molecular evolution 1. History of life on Earth 2. Genes as chronometers 3. Tree of life II. Molecular ecology 1. Prokaryotic abundance
Main Topics. Microbial habitats. Microbial habitats. Lecture 21: Bacterial diversity and Microbial Ecology. Ecological characteristics of bacteria
Lecture 21: Bacterial diversity and Microbial Ecology Dr Mike Dyall-Smith Haloarchaea Research Lab., Lab 3.07 mlds@unimelb.edu.au Ref: Prescott, Harley & Klein, 6th ed., parts of chapters 21-24 (refer
Expressed genes profiling (Microarrays) Overview Of Gene Expression Control Profiling Of Expressed Genes
Expressed genes profiling (Microarrays) Overview Of Gene Expression Control Profiling Of Expressed Genes Genes can be regulated at many levels Usually, gene regulation, are referring to transcriptional
Contents. 1 Basic Molecular Microbiology of Bacteria... 1 Exp. 1.1 Isolation of Genomic DNA Introduction Principle...
Contents 1 Basic Molecular Microbiology of Bacteria... 1 Exp. 1.1 Isolation of Genomic DNA... 1 Introduction... 1 Principle... 1 Reagents Required and Their Role... 2 Procedure... 3 Observation... 4 Result
Classification of Microorganisms
PowerPoint Lecture Presentations prepared by Bradley W. Christian, McLennan Community College C H A P T E R 10 Classification of Microorganisms The Study of Phylogenetic Relationships Taxonomy is the science
Microbial cycling of dissolved organic matter
Microbial cycling of dissolved organic matter MOG Lecture 8 March 1 st, 2011 8.1 Microbial cycling of dissolved organic matter The inclusion of microbes in marine food webs Microbial production (from 2
Gene expression profiling experiments:
Gene expression profiling experiments: Problems, pitfalls, and solutions. Heli Borg The Alternatives in Microarray Experiments bacteria - eucaryots non poly(a) + - poly(a) + oligonucleotide Affymetrix
Single cell analysis of bacteria: activity and genomics
Single cell analysis of bacteria: activity and genomics Single cell activity - CTC Lysotracker for heterotrophic protists Matching phylogeny and metabolism - one cell at a time Conceptualization Most marine
Probing Phytoplankton Biodiversity. Isabelle C. Biegala (IRD, Marseille, France)
Probing Phytoplankton Biodiversity Isabelle C. Biegala (IRD, Marseille, France) The content of this presentation is the exclusive property of its author. Any use is prohibited. If you wish to use any material
MMG 301 Dr. Frank Dazzo Microbial Ecology: Methodology & Soil Microbiology. Some methods used to study microbes in natural habitats:
MMG 301 Dr. Frank Dazzo Microbial Ecology: Methodology & Soil Microbiology Some methods used to study microbes in natural habitats: Microbial abundance: microscopy, computer-assisted image analysis measurement
Earthworms, efficient soil engineers
Functional Ecology Department, University of Rennes, Brittany, France Biotic Control of Atrazine Degradation in Soils: Bacterial Functional Diversity of Degradation and Macrofauna Effects MONARD Cécile
Chapter 4B: Methods of Microbial Identification. Chapter Reading pp , ,
Chapter 4B: Methods of Microbial Identification Chapter Reading pp. 118-121, 244-245, 250-251 Biochemical Testing In addition to morphological (i.e., appearance under the microscope) and differential staining
including, but not limited to:
*This Section is part of the original Request for Proposal # P08 080. The Contractor should provide the following eligible Scientific Biomedical Research Equipment, Reagents & Supplies including, but not
Genomic and Proteomic Analysis of Deep Underground Microbial Communities
Genomic and Proteomic Analysis of Deep Underground Microbial Communities Kenneth F. Reardon, Chemical & Biological Engineering, Colorado State University Amy Pruden, Civil & Environmental Engineering,
The effect of agricultural nutrient loading on estuarine bacterioplankton communities
The effect of agricultural nutrient loading on estuarine bacterioplankton communities Jude Apple Horn Point Laboratory University of Maryland Center for Environmental Science Cambridge, Maryland What is
Methods of Biomaterials Testing Lesson 3-5. Biochemical Methods - Molecular Biology -
Methods of Biomaterials Testing Lesson 3-5 Biochemical Methods - Molecular Biology - Chromosomes in the Cell Nucleus DNA in the Chromosome Deoxyribonucleic Acid (DNA) DNA has double-helix structure The
DNA Microarray Technology
2 DNA Microarray Technology 2.1 Overview DNA microarrays are assays for quantifying the types and amounts of mrna transcripts present in a collection of cells. The number of mrna molecules derived from
Importance. Prokaryotes vs. Eukaryotes. Viruses: a form of life or not?
1 Importance Microorganisms (esp. bacteria) plays a key role in the decomposition and stabilization of organic matter Control of diseases caused by pathogenic organisms of human origin Prokaryotes vs.
Feasibility-report of a quantitative method for rapid assessment of microbial population composition in drinking water using flowcytometry
Techneau, 06. June 2007 Feasibility-report of a quantitative method for rapid assessment of microbial population composition in drinking water using flowcytometry combined with FISH Deliverable 3.3.2 Techneau,
Imaging the immune system with a Two photon (2P) microscope
Imaging the immune system with a Two photon (2P) microscope QuickTime et un décompresseur Cinepak sont requis pour visionner cette image. Main advantages of 2P microscopy : 1/ Deep penetration into tissue
Marine Microbial Processes
Marine Microbial Processes Outline size-structured food webs brief history of the development of our current understanding of microbially dominated food webs carbon cycling in marine food webs evolving
Microbial biomass in the sea: Methods, limitations, and distributions
Microbial biomass in the sea: Methods, limitations, and distributions Matt Church (MSB 612 / 956-8779 / mjchurch@hawaii.edu) Marine Microplankton Ecology OCN 626 E. F. DeLong The tripartite goals of microbial
Preparation and characterization of nanostructured thermoelectric materials
QuickTime et un décompresseur TIFF (non compressé) sont requis pour visionner cette image. Preparation and characterization of nanostructured thermoelectric materials Laboratoire de Physique des Matériaux,
Gene Expression Technology
Gene Expression Technology Bing Zhang Department of Biomedical Informatics Vanderbilt University bing.zhang@vanderbilt.edu Gene expression Gene expression is the process by which information from a gene
Eva Sintes* and Gerhard J. Herndl
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Nov. 2006, p. 7022 7028 Vol. 72, No. 11 0099-2240/06/$08.00 0 doi:10.1128/aem.00763-06 Copyright 2006, American Society for Microbiology. All Rights Reserved. Quantifying
Characterizing Phenotypes of Bacteria by Staining Method
Experiment 3 Laboratory to Biology III Diversity of Microorganisms / Wintersemester / page 1 Experiment 3 Characterizing Phenotypes of Bacteria by Staining Method Advisor NN Reading Chapters in BBOM 9
Characterizing Phenotypes of Bacteria by Staining Method
Experiment 3 Laboratory to Biology III Diversity of Microorganisms / Wintersemester / page 1 Experiment Characterizing Phenotypes of Bacteria by Staining Method Advisor Reading NN Chapters 3.1, 3.7, 3.8,
Comparative assessment of epifluorescence microscopy, flow cytometry and solid-phase cytometry used in the enumeration of specific bacteria in water
AQUATIC MICROBIAL ECOLOGY Vol. 25: 301 309, 2001 Published September 28 Aquat Microb Ecol Comparative assessment of epifluorescence microscopy, flow cytometry and solid-phase cytometry used in the enumeration
Visualizing Cells Molecular Biology of the Cell - Chapter 9
Visualizing Cells Molecular Biology of the Cell - Chapter 9 Resolution, Detection Magnification Interaction of Light with matter: Absorbtion, Refraction, Reflection, Fluorescence Light Microscopy Absorbtion
C. acetobutyricum. Initial co-culture. Transfer 1
a Co-culture C. acetobutyricum b Initial co-culture Transfer 1 Transfer 2 c Supplementary Figure 1: Persistence of the bacteria in co-culture. (a) Specificity of genes used to follow and in pure culture
Combining Techniques to Answer Molecular Questions
Combining Techniques to Answer Molecular Questions UNIT FM02 How to cite this article: Curr. Protoc. Essential Lab. Tech. 9:FM02.1-FM02.5. doi: 10.1002/9780470089941.etfm02s9 INTRODUCTION This manual is
LysoTracker Red DND-99 (Invitrogen) was used as a marker of lysosome or acidic
information MATERIAL AND METHODS Lysosome staining LysoTracker Red DND-99 (Invitrogen) was used as a marker of lysosome or acidic compartments, according to the manufacturer s protocol. Plasmid independent
BIOTECHNOLOGY. Course Syllabus. Section A: Engineering Mathematics. Subject Code: BT. Course Structure. Engineering Mathematics. General Biotechnology
BIOTECHNOLOGY Subject Code: BT Course Structure Sections/Units Section A Section B Unit 1 Unit 2 Unit 3 Unit 4 Unit 5 Unit 6 Unit 7 Section C Section D Section E Topics Engineering Mathematics General
Are the actively respiring cells (CTC+) those responsible for bacterial production in aquatic environments?
Are the actively respiring cells (CTC+) those responsible for bacterial production in aquatic environments? Pierre Servais, Hélène Agogué, Claude Courties, Fabien Joux, Philippe Lebaron To cite this version:
Day 3. Examine gels from PCR. Learn about more molecular methods in microbial ecology
Day 3 Examine gels from PCR Learn about more molecular methods in microbial ecology Genes We Targeted 1: dsrab 1800bp 2: mcra 750bp 3: Bacteria 1450bp 4: Archaea 950bp 5: Archaea + 950bp 6: Negative control
School of Fisheries, Aquaculture and Aquatic Sciences
School of Fisheries, Aquaculture and Aquatic Sciences 1 School of Fisheries, Aquaculture and Aquatic Sciences Fisheries science combines a general foundation in chemistry, mathematics and biological sciences
Mochamad Nurcholis. Food Technology Department Agricuktural Technology Faculty Brawijaya University 2013
Mochamad Nurcholis Food Technology Department Agricuktural Technology Faculty Brawijaya University 2013 Microbial Identification Type Conventional Identification DNA Hybridization PCR Amplification DNA
Comparison of Blue Nucleic Acid Dyes for Flow Cytometric Enumeration of Bacteria in Aquatic Systems
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, May 1998, p. 1725 1730 Vol. 64, No. 5 0099-2240/98/$04.00 0 Copyright 1998, American Society for Microbiology Comparison of Blue Nucleic Acid Dyes for Flow Cytometric
Fundamentals and Applications of Biofilms Analysis, Structure and Physiology of Bacterial Biofilms Ching-Tsan Huang ( 黃慶璨 ) Office: Agronomy
1 Fundamentals and Applications of Biofilms Analysis, Structure and Physiology of Bacterial Biofilms Ching-Tsan Huang ( 黃慶璨 ) Office: Agronomy Building, Room 111 Tel: (02) 33664454 E-mail: cthuang@ntu.edu.tw
What shapes the metabolic and phylogenetic structure of microbial communities in aquatic systems?
What shapes the metabolic and phylogenetic structure of microbial communities in aquatic systems? What is a microbial food web and what is the ecological importance? Marine Microbial Food Web Dynamics
10. BIOTECHNOLOGY (Code No. 045)
10. BIOTECHNOLOGY (Code No. 045) An unprecedented growth of human knowledge in the field of Biological Sciences coupled with equally significant developments in the field of technology have brought significant
Bacterial Abundance. Objective Measure bacterial numbers and mass per unit volume. Note, we are not concerned with identification here.
Bacterial Abundance Objective Measure bacterial numbers and mass per unit volume. Note, we are not concerned with identification here. Why do we want to know abundance? Allows determination of biomass
Biotechnology. Chapter 20. Biology Eighth Edition Neil Campbell and Jane Reece. PowerPoint Lecture Presentations for
Chapter 20 Biotechnology PowerPoint Lecture Presentations for Biology Eighth Edition Neil Campbell and Jane Reece Lectures by Chris Romero, updated by Erin Barley with contributions from Joan Sharp Copyright
Biology 201 (Genetics) Exam #3 120 points 20 November Read the question carefully before answering. Think before you write.
Name KEY Section Biology 201 (Genetics) Exam #3 120 points 20 November 2006 Read the question carefully before answering. Think before you write. You will have up to 50 minutes to take this exam. After
Selected Techniques Part I
1 Selected Techniques Part I Gel Electrophoresis Can be both qualitative and quantitative Qualitative About what size is the fragment? How many fragments are present? Is there in insert or not? Quantitative
Contents... vii. List of Figures... xii. List of Tables... xiv. Abbreviatons... xv. Summary... xvii. 1. Introduction In vitro evolution...
vii Contents Contents... vii List of Figures... xii List of Tables... xiv Abbreviatons... xv Summary... xvii 1. Introduction...1 1.1 In vitro evolution... 1 1.2 Phage Display Technology... 3 1.3 Cell surface
BIMM 121 Learning Goals, Outcomes, Assessments, Practice
BIMM 121 Learning Goals, Outcomes, Assessments, Practice Learning Goals: tudents will: A. General cientific/lab kills and Competencies 1. Learn the importance of designing and using the right controls
Basic Principles in Flow Cytometry. Flow Cytometry
Basic Principles in Flow Cytometry Flow Cytometry» Flow Cytometry is the technological process that allows for the individual measurements of cell fluorescence and light scattering. This process is performed
B. Incorrect! Ligation is also a necessary step for cloning.
Genetics - Problem Drill 15: The Techniques in Molecular Genetics No. 1 of 10 1. Which of the following is not part of the normal process of cloning recombinant DNA in bacteria? (A) Restriction endonuclease
Bacterial Abundance. Objective Measure bacterial numbers and mass per unit volume. Note, we are not concerned with identification here.
Bacterial Abundance Objective Measure bacterial numbers and mass per unit volume. Note, we are not concerned with identification here. Why do we want to know abundance? Allows determination of biomass
Fluorescent In Situ Hybridization (FISH) Assay
Fluorescent In Situ Hybridization (FISH) Assay 1 What is FISH 2 Probes 3 FISH Procedure 4 Application Definition, Principle and Sample Types The core of FISH technology A quick and simple FISH protocol
Lecture 22 Eukaryotic Genes and Genomes III
Lecture 22 Eukaryotic Genes and Genomes III In the last three lectures we have thought a lot about analyzing a regulatory system in S. cerevisiae, namely Gal regulation that involved a hand full of genes.
An integrative academic-industry collaboration for biotechnology driven discovery of new functions in marine environmental metagenomes
An integrative academic-industry collaboration for biotechnology driven discovery of new functions in marine environmental metagenomes Renzo Kottmann Microbial Genomics and Bioinformatics Research Group
SGN-6106 Computational Systems Biology I
SGN-6106 Computational Systems Biology I A View of Modern Measurement Systems in Cell Biology Kaisa-Leena Taattola 21.2.2007 1 The cell a complex system (Source: Lehninger Principles of Biochemistry 4th
Departments of Biotechnology. Academic planner for (ODD semesters) Month I Semester III Semester V Semester
Departments of Biotechnology Academic planner for 2014-15 (ODD semesters) Subject: Biotechnology Month I Semester III Semester V Semester JULY AUGUST Cell as a Basic unit of Living Systems, Discovery of
Bacteria in Semen. Sponsored by Bedford Research Foundation
Bacteria in Semen Background: The concept of detecting and identifying bacteria by ribosomal RNA gene sequences is about 15 years old. Although limited, the application of this approach to clinical specimens
King George's Medical University UP., Lucknow center for advance research
SUMMER/WINTER: SHORT TERM TRAINING COURSE-DRAFT DURATION: 30 DAYS Molecular Biology Unit-Genomics 10:00 to 12:00 AM 2:00 to 4:00 PM Lecture 1: Basics of Molecular Biology- DNA and RNA Blood Collection
Bioinformatics: Microarray Technology. Assc.Prof. Chuchart Areejitranusorn AMS. KKU.
Introduction to Bioinformatics: Microarray Technology Assc.Prof. Chuchart Areejitranusorn AMS. KKU. ความจร งเก ยวก บ ความจรงเกยวกบ Cell and DNA Cell Nucleus Chromosome Protein Gene (mrna), single strand
Innovations in Molecular Lab Operation. Joseph M. Campos, PhD, D(ABMM), F(AAM)
Innovations in Molecular Lab Operation Lessons in the Best Ways to Blend Automation, Lean Workflow, and Paperless Processes Joseph M. Campos, PhD, D(ABMM), F(AAM) Interim Chief, Division of Laboratory
Why carbon? The Struggle for Composition 9/9/2010. Microbial biomass in the sea: Methods, limitations, and distributions
Microbial biomass in the sea: Methods, limitations, and distributions Key concepts in biological oceanography Matt Church (MSB 614 / 956-8779 / mjchurch@hawaii.edu) Marine Microplankton Ecology OCN 626
STUDY GUIDE SECTION 13-1 DNA Technology
STUDY GUIDE SECTION 13-1 DNA Technology Name Period Date Multiple Choice-Write the correct letter in the blank. 1. To cut DNA molecules into pieces at specific sequences of nucleotides, genetic engineers
Background. Biogeochemical Role. Archaeal Nitrification in the Ocean by Cornelia Wutcher et al Presenters: Brian Drupieski and Megan McCurdy
Archaeal Nitrification in the Ocean by Cornelia Wutcher et al. 2006. Presenters: Brian Drupieski and Megan McCurdy Background Crenarchaeota From the kingdom Archaea Most abundant oceanic prokaryote Limited
Microbiology. Zhenmei Lu ( 吕镇梅 ) 2010 Spring-Summer 2017 Spring-Summer
Microbiology Zhenmei Lu ( 吕镇梅 ) lzhenmei@zju.edu.cn 2010 Spring-Summer 2017 Spring-Summer Chapter 23 Microbial Ecosystems 23.1 Ecological Concepts Ecosystem: a dynamic complex of plant, animal, and microbial
Supporting Information for
Supporting Information for Building Electromagnetic Hot Spots in Living Cells via Target-Triggered Nanoparticle Dimerization Wen Zhou, 1,2 Qiang Li, 1 Huiqiao Liu, 1 Jie Yang, 1 Dingbin Liu 1,2 * 1. College
Applications and Uses. (adapted from Roche RealTime PCR Application Manual)
What Can You Do With qpcr? Applications and Uses (adapted from Roche RealTime PCR Application Manual) What is qpcr? Real time PCR also known as quantitative PCR (qpcr) measures PCR amplification as it
Taxonomy. Classification of microorganisms 3/12/2017. Is the study of classification. Chapter 10 BIO 220
Taxonomy Is the study of classification Organisms are classified based on relatedness to each other Chapter 10 BIO 220 Fig. 10.1 1 Species Binomial nomenclature for species identification A eukaryotic
MOLECULAR TYPING TECHNIQUES
MOLECULAR TYPING TECHNIQUES RATIONALE Used for: Identify the origin of a nosocomial infection Identify transmission of disease between individuals Recognise emergence of a hypervirulent strain Recognise
Molecular Cell Biology - Problem Drill 11: Recombinant DNA
Molecular Cell Biology - Problem Drill 11: Recombinant DNA Question No. 1 of 10 1. Which of the following statements about the sources of DNA used for molecular cloning is correct? Question #1 (A) cdna
Overview Microscale Applica0ons Group. Mar 24, 2015
Overview Microscale Applica0ons Group Mar 24, 2015 We need ng s of DNA to sequence a microbial genome How do we study the uncultured majority? cul%vated uncul%vated Single Cell Genomics Pipeline Most microbes
Page 1. AMPTask Force on MLS Molecular Pathology Curriculum Dear Molecular Diagnostics Laboratory Manager,
AMPTask Force on MLS Molecular Pathology Curriculum Dear Molecular Diagnostics Laboratory Manager, Recently the Association for Molecular Pathology (AMP) has decided to develop a suggested curriculum in
DOI: 10.1038/ncb3259 A Ismail et al. Supplementary Figure 1 B 60000 45000 SSC 30000 15000 Live cells 0 0 15000 30000 45000 60000 FSC- PARR 60000 45000 PARR Width 30000 FSC- 15000 Single cells 0 0 15000
SIMS2003. Instructors:Rus Yukhananov, Alex Loguinov BWH, Harvard Medical School. Introduction to Microarray Technology.
SIMS2003 Instructors:Rus Yukhananov, Alex Loguinov BWH, Harvard Medical School Introduction to Microarray Technology. Lecture 1 I. EXPERIMENTAL DETAILS II. ARRAY CONSTRUCTION III. IMAGE ANALYSIS Lecture
MOLECULAR STRUCTURE OF DNA
MOLECULAR STRUCTURE OF DNA Characteristics of the Genetic Material 1. Replication Reproduced and transmitted faithfully from cell to cell (generation to generation) 2. Information Storage Biologically
Executive Summary. clinical supply services
clinical supply services case study Development and NDA-level validation of quantitative polymerase chain reaction (qpcr) procedure for detection and quantification of residual E.coli genomic DNA Executive
POPULATION- RELATED PROCESSES
THE ROLE OF FLOW CYTOMETRY IN MARINE BIODIVERSITY AND ECOSYSTEM FUNCTION UNTREATED WATER FLUORESCENT DYE PRESERVED WATER FLUORESCENT PROBE POPULATION- OUTPUTS2 OUTPUTS1 FLOW MULTI- SORTING DATA VARIATE
Infectious Disease Omics
Infectious Disease Omics Metagenomics Ernest Diez Benavente LSHTM ernest.diezbenavente@lshtm.ac.uk Course outline What is metagenomics? In situ, culture-free genomic characterization of the taxonomic and
A Supersandwich Fluorescence in Situ Hybridization (SFISH) Strategy. for Highly Sensitive and Selective mrna Imaging in Tumor Cells
Electronic Supplementary Material (ESI) for ChemComm. This journal is The Royal Society of Chemistry 2015 Electronic Supplementary Information (ESI) A Supersandwich Fluorescence in Situ Hybridization (SFISH)
Lab 2-Microbial Enumeration
Lab 2-Microbial Enumeration 2/19/08 CE 573 Introduction There are many different techniques that can be utilized when trying to quantify microorganisms found in a given sample. The purpose of this lab
Chapter 20 Biotechnology
Chapter 20 Biotechnology Manipulation of DNA In 2007, the first entire human genome had been sequenced. The ability to sequence an organisms genomes were made possible by advances in biotechnology, (the
DNA/RNA MICROARRAYS NOTE: USE THIS KIT WITHIN 6 MONTHS OF RECEIPT.
DNA/RNA MICROARRAYS This protocol is based on the EDVOTEK protocol DNA/RNA Microarrays. 10 groups of students NOTE: USE THIS KIT WITHIN 6 MONTHS OF RECEIPT. 1. EXPERIMENT OBJECTIVE The objective of this
ESOF2010,Torino 2-7 July
1 From Life Science to Environmental Science: Successes and Challenges Teresa Lettieri European Commission Joint Research Centre; Institute for Environment and Sustainability http://ies.jrc.ec.europa.eu/
Supplementary Figure S1 Supplementary Figure S2 Supplementary Figure S3. Supplementary Figure S4
Supplementary Figure S1 Supplementary Figure S2 Supplementary Figure S3 Supplementary Figure S4 Supplementary Figure S5 Supplementary Figure S6 Supplementary Figure S7 Supplementary Figure S8 Supplementary
ANAT 3231 Cell Biology Lab12 Stem Cell Analysis
ANAT 3231 Cell Biology Lab12 Stem Cell Analysis 2 June 2010 Dr Antonio Lee Neuromuscular & Regenera9ve Medicine Unit School of Medical Sciences, UNSW Introduction to Flow Cytometry Contributed by Vittoria
Computers in Biology and Bioinformatics
Computers in Biology and Bioinformatics 1 Biology biology is roughly defined as "the study of life" it is concerned with the characteristics and behaviors of organisms, how species and individuals come
Use of molecular methods for the assessment of microbial community composition and dynamics in Alpine Lakes.
Use of molecular methods for the assessment of microbial community composition and dynamics in Alpine Lakes. Ursula Dorigo, Stéphan Jacquet, Dominique Fontvieille, & Jean-François Humbert UMR CARRTEL Equipe
Assays for gene expression and protein production
Assays for gene expression and protein production Module 3, Lecture 5! 20.109 Spring 2011! Topics for Lecture 5 Measuring protein levels! Measuring transcript levels! Imaging assays! 2 Module overview:
ALP (alkaline phosphatase) calibrators were analyzed manually in microtiter plates to find the linearity range by following this protocol:
Exam Mol 3008 May 2009 Subject 1 (15p) ALP (alkaline phosphatase) calibrators were analyzed manually in microtiter plates to find the linearity range by following this protocol: Reaction solutions: 50
Measuring gene expression
Measuring gene expression Grundlagen der Bioinformatik SS2018 https://www.youtube.com/watch?v=v8gh404a3gg Agenda Organization Gene expression Background Technologies FISH Nanostring Microarrays RNA-seq
Soil resources and soil microbial processes in agro-environments Aiming at improved agricultural. practices
Sustainable cultivation methods for agriculture increasing soil crop cover and optimizing ecosystem services of soil microorganisms (SUCCESS) Soil resources and soil microbial processes in agro-environments
Methods in Microbial Ecology
LECTURE PRESENTATIONS For BROCK BIOLOGY OF MICROORGANISMS, THIRTEENTH EDITION Michael T. Madigan, John M. Martinko, David A. Stahl, David P. Clark Chapter 22 Lectures by John Zamora Middle Tennessee State
Biology (with a concentration in Biochemistry and Cellular and Molecular Biology) University of Tennessee, Knoxville Awarded in May 2001
Business Address: 101-A Science & Engineering Bldg University of Tennessee Knoxville, TN 37996 Business Phone: (865) 974 9202 Steven C. Minkin Jr. Home address: 6506 Westland Dr. Knoxville, TN 37919 Phone
QuickTime et un décompresseur Graphismes sont requis pour visionner cette image.
QuickTime et un décompresseur Graphismes sont requis pour visionner cette image. Dust Emission by Powders Renaud ANSART, John DODDS, Alain De RYCK Centre RAPSODEE Ecole des Mines d Albi, France QuickTime