Finding A Place for A New Microbial Biopesticide in Crop Protection Programs

Transcription

1 Finding A Place for A New Microial Biopesticide in Crop Protection Programs 2 nd Ag Innovations Conference - La Jolla, CA (13 Aug 2017) Michael B. Dimock, Ph.D. Vice President, Field Development Certis USA, L.L.C. Columia, Maryland

2 Who is Certis USA? Biopesticide developer, manufacturer & marketer Wholly owned y Mitsui & Co. (Fortune 500) Broad product portfolio (~30 active ingredients) Microials, otanicals, iochemicals, others Agriculture, Ornamentals, Specialty, Home Garden Sales in over 50 countries, all continents except Antarctica Headquarters and R&D: Columia, MD Manufacturing sites: Wasco, CA (liquid fermentation) Bangalore, India (neem extraction) Butte, MT (solid fermentation) Dedicated North America field sales, technical & regulatory team More info:

3 Active ingredient: Bacillus mycoides isolate J (BmJ) Common species of acterium in soil and plant samples worldwide. Isolate J from asymptomatic sugar eet foliage in field with severe Cercospora outreak in Montana (Dr. Barry Jacosen). Patented y Montana State University, licensed to Certis USA. Fermentation methods, scale-up and formulation developed y Certis. B. Jacosen

4 Field trials y B. Jacosen (Montana State Univ.) & collaorators, Treatments: 1. Best laeled fungicide rotation (4 applications total). 2. BmJ alone (4 applications at cfu/ml) 3. BmJ + ½ rate fungicide at disease onset, then 3 more BmJ applications. Cercospora leaf spot in sugar eet % Efficacy vs. Untreated Check Triazoles Stroilurins Mean ± SEM over 11 growing seasons Same disease control with fewer fungicide applications 0 Standard fungicide program BmJ only BmJ + reduced fungicide program

5 ASM Pathogen BmJ BmJ Mode of Action Chemical elicitors Cytosol Nonexpresser of Pathogenesis Related Protein 1 Nucleus NPR1 NPR1 TF PAMPs Oxidative urst (ROS) Salicylic Acid (SA) NPR1 NPR1 NPR1 NPR1 NPR1 PR genes Inactive oligomer Active monomers NPR1 MAMPs? Defense proteins Little or no direct antagonistic effect on plant pathogens. Activates plant s natural defenses against pathogens. Genetic and iochemical pathway similar to acienzolar-s-methyl (Actigard ). NPR1 - dependent SA - independent New FRAC group: P6 (microial inducer of plant resistance). Induced resistance response detectale within 3 hours, lasts days. No phytotoxicity in 20+ years of field trials. Adapted from: Bargaus et al, Physiol. Mol. Plant Pathol. 61: Bargaus-Larson & Jacosen, J. Sugar Beet Res. 44: Fu & Dong, Ann. Rev. Plant Biol. 64: Actigard is a registered trademarks of Syngenta Crop Protection, Inc.

6 Registration/Laeling: EPA & PMRA approved late 2016 OMRI listed (USA) ECOCERT (Canada) Exempt from residue tolerance - no PHI or MRL Water-dispersile granule (WG) 40% active ingredient Minimum 30 illion viale spores per gram Application rate: ounces/acre Target concentration: 4.5 oz/100 gal (= 10 7 spores/ml) Application methods Ground sprayer Aerial application (USA) Stationary overhead sprinkler chemigation See product lael for specific use instructions and other information.

7 Potato Early Blight RAUDPC a Aerdeen, ID CER Efficacy (% reduction relative to UTC) indicated for each treatment. 1.0 LG replaced 2 fungicide apps Untreated check 70% 1.2 oz/a (ABCD) 83% Fungicide 1 (AB) Fungicide 2 (CD) 87% (AC) Fungicide 1 (B) Fungicide 2 (D) Four applications on a 14-day interval y ATV-mounted R&D spray oom delivering 25 GPA at 30 psi through 2 nozzles/row. RAUDPC = Relative Area Under the Disease Progress Curve.

8 Potato Late Blight 2,000 1,500 1, AUDPC a Untreated check a 5% 3 oz/a 73% Fungicide Full rate Rock Springs, PA CER Efficacy (% reduction vs. UTC) indicated for each treatment. 65% 3 oz + Fungicide 1/2 rate 5 weekly applications y tractor-mounted side oom sprayer (45 GPA at 30 psi, 3 nozzles/row). Natural infection augmented y artificial inoculation with P. infestans (US-23). Although not effective alone, LifeGard replaced half of total fungicide when used in tank mix, without loss of efficacy. Final foliar light severity (%) 100 a Untreated check c 99% Standard fungicide (8 apps) ½ frequency (no LG) 68% Standard fungicide (4 apps) Weekly applications y ATV-mounted spray oom (25 GPA at 80 psi, 3 nozzles/row). Artificially inoculated with P. infestans (US-22). Clarksville, MI CER c 92% Std fung. alt oz (4 apps ea.) LifeGard alt. std. fungicide was as effective as full fungicide program. Reduced fungicide program w/o LifeGard was not.

9 Grape Downy Mildew Final disease severity on fruit (%) - Chardonnay variety a Efficacy (% reduction vs. UTC) indicated for each treatment. Reduced freq. (no LifeGard) Geneva, NY CER Untreated check c c 99% 90% only (7 apps) (4 apps) alt. Std Fung. (3 apps) 67% Standard fungicides (4 apps) c 86% Full fungicide program (7 apps) 7 applications at 14d intervals with hooded oom sprayer (50 GPA pre- & 100 GPA post-loom). LifeGard applied at 4.5 oz/100 gal (10 7 cfu/ml). Disease severity evaluated on 20 fruit clusters at harvest. LifeGard was as efficacious in stand-alone applications as a grower s standard fungicide program. Reducing frequency of fungicide application y alternating with LifeGard resulted in the same efficacy as the full fungicide program. Reducing fungicide frequency without LifeGard resulted in more disease.

10 Downy Mildew in Leafy Veg Spinach - Final severity (%) 12 DAA5 a Efficacy (% reduction relative to UTC) indicated for each treatment. a Guadalupe, CA CER Head Lettuce - AUDPC Guadalupe, CA CER % 39% 44% % 67% 0 Untreated check Fung 1 (AC) Fung 2 (BD) Fung 3 (E) (AE) Fung 2 (BD) Fung 1 (C) (ACE) Fung 2 (B) Fung 1 (D) Weekly appl. y CO 2 ackpack (40-50 GPA, 3 nozzles). Inclusion of LifeGard in rotation with standard fungicides resulted in improved disease control with fewer total fungicide applications. 0 Untreated check Fung 2 (ADG) Blockade (BEH) Fung 1 (CF) Fung 2 (ADG) (BEH) Fung 1 (CF) Weekly appl. y CO 2 ackpack (40-50 GPA, 3 nozzles). Products and rates per acre: LifeGard (BmJ): 2.25 oz (10 7 cfu/ml) Blockade* (acienzolar-s-methyl): 0.75 oz LifeGard performed similar to Blockade in a rotation with standard fungicides. *Blockade is a registered trademarks of Syngenta Crop Protection, Inc.

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