Course Syllabus Microbiology BIOL 2420 Spring 2014 (January 13 May 11) 75877

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1 Semester with Course Reference Number (CRN) Instructor contact information (phone number and address) Office Location and Hours Course Location/Times Course Semester Credit Hours (SCH) (lecture, lab) If applicable Course Syllabus Microbiology BIOL 2420 Spring 2014 (January 13 May 11) Dr. Olumide Ogunmosin (713) Northline Campus Room 321 Mon and Wed 10:00am 11:00am Tue and Thur 10:00am 11:00am Northeast College - Northline Campus Tuesday Lecture 02:00pm 05:00pm Rm 309 Thursday Laboratory 02:00pm 05:00pm Rm 217 Credit Hours: 4 Lecture Hours: 3 Laboratory Hours: 3 Total Course Contact Hours Course Length (number of weeks) Type of Instruction In-Class Lecture (web enhanced) Course Description: Course Prerequisite(s) Study of the morphology, physiology, and taxonomy of representative groups of pathogenic and nonpathogenic Pure cultures of microorganisms grown on selected media are used in learning laboratory techniques. Includes a brief overview of food microbes, public health, and immunology. Core Curriculum course PREREQUISITE(S): BIOL 1406 College-level reading (or take GUST 0342) and College-level writing (or take ENGL 0310/0349)

2 Academic Discipline/CTE Program Learning Outcomes PSLO#1 - Will display an understanding of biological systems and evolutionary processes spanning all ranges of biological complexity, including atoms, molecules, genes, cells, and organisms. PSLO#2 - Will integrate factual and conceptual information into an understanding of scientific data by written, oral and/or visual communication. (This may include successful completion of a course-specific research project or a case study module). PSLO#3 - Will demonstrate proficiency and safe practices in the use of laboratory equipment and basic laboratory techniques. PSLO#4 - Will apply principles of the scientific method to problems in biology in the collection, recording, quantitative measurement, analysis and reporting of scientific data. Course Student Learning Outcomes (SLO): Bio LECTURE 1. Provide examples of the impact of microorganisms on agriculture, environment, ecosystem, energy, and human health, including biofilms. 2. Identify unique structures, capabilities, and genetic information flow of 3. Compare the life cycles and structures of different types of viruses. 4. Discuss how microscopy has revealed the structure and function of 5. Give examples of the range of metabolic diversity exhibited by microorganisms, impact of metabolic characteristics on growth, and control of growth. 6. Describe the causes and consequences of mutations on microbial evolution and the generation of diversity as well as human impacts on adaptation. 7. Classify interactions of microorganisms on human and non-human hosts as neutral, detrimental, or beneficial. Bio LABORATORY 1. Apply scientific reasoning to investigate questions and utilize scientific tools such as microscopes and laboratory equipment to collect and analyze data. 2. Use critical thinking and scientific problem-solving to make informed decisions in the laboratory. 3. Communicate effectively the results of scientific investigations. 4. Identify unique structures and capabilities of 5. Compare the life cycles of different types of viruses. 6. Discuss how microscopy has revealed the structure and function of 7. Give examples of the range of metabolic diversity exhibited by microorganisms, impact of metabolic characteristics on growth, and control of growth. 8. Classify interactions of microorganisms on human and non-human hosts as neutral, detrimental, or beneficial. Learning Objectives (Numbering system should be linked to SLO - Bio LECTURE 1. Provide examples of the impact of microorganisms on agriculture, environment, ecosystem, energy, and human health, including biofilms. 2. Identify unique structures, capabilities, and genetic information flow of

3 e.g., 1.1, 1.2, 1.3, etc.) 3. Compare the life cycles and structures of different types of viruses. 4. Discuss how microscopy has revealed the structure and function of 5. Give examples of the range of metabolic diversity exhibited by microorganisms, impact of metabolic characteristics on growth, and control of growth. 6. Describe the causes and consequences of mutations on microbial evolution and the generation of diversity as well as human impacts on adaptation. 7. Classify interactions of microorganisms on human and non-human hosts as neutral, detrimental, or beneficial. Bio LABORATORY 1. Apply scientific reasoning to investigate questions and utilize scientific tools such as microscopes and laboratory equipment to collect and analyze data. 2. Use critical thinking and scientific problem-solving to make informed decisions in the laboratory. 3. Communicate effectively the results of scientific investigations. 4. Identify unique structures and capabilities of 5. Compare the life cycles of different types of viruses. 6. Discuss how microscopy has revealed the structure and function of 7. Give examples of the range of metabolic diversity exhibited by microorganisms, impact of metabolic characteristics on growth, and control of growth. 8. Classify interactions of microorganisms on human and non-human hosts as neutral, detrimental, or beneficial. SCANS and/or Core Curriculum Competencies: If applicable Instructional Methods Student Assignments Core Curriculum Competencies: Lecture exams, laboratory practical exams, and class activities will enhance the learning process by giving the student the opportunity to demonstrate the basic intellectual competencies of reading, writing, speaking, listening, and showing critical thinking and problem solving ability. In-Class Lecture (web enhanced) Bio LECTURE 1. Provide examples of the impact of microorganisms on agriculture, environment, ecosystem, energy, and human health, including biofilms. 2. Identify unique structures, capabilities, and genetic information flow of 3. Compare the life cycles and structures of different types of viruses. 4. Discuss how microscopy has revealed the structure and function of 5. Give examples of the range of metabolic diversity exhibited by microorganisms, impact of metabolic characteristics on growth, and control of growth. 6. Describe the causes and consequences of mutations on microbial

4 evolution and the generation of diversity as well as human impacts on adaptation. 7. Classify interactions of microorganisms on human and non-human hosts as neutral, detrimental, or beneficial. (Includes immunity) Bio LABORATORY 1. Apply scientific reasoning to investigate questions and utilize scientific tools such as microscopes and laboratory equipment to collect and analyze data. 2. Use critical thinking and scientific problem-solving to make informed decisions in the laboratory. 3. Communicate effectively the results of scientific investigations. 4. Identify unique structures and capabilities of 5. Compare the life cycles of different types of viruses. 6. Discuss how microscopy has revealed the structure and function of 7. Give examples of the range of metabolic diversity exhibited by microorganisms, impact of metabolic characteristics on growth, and control of growth. 8. Classify interactions of microorganisms on human and non-human hosts as neutral, detrimental, or beneficial. (Includes immunity) Student Assessment(s) Explain microbial evolution, microbial diversity on earth, and environmental impact of microbes. Chapter assessment Understand microbial cell biology and genetics including, cellular structure and function, cell division and growth, metabolism, mutations, and inheritance. Chapter assessment Recognize Microbe-Human Interactions including, host non-specific defenses, adaptive immunity, chemotherapy (antibiotics), pathogenesis, and disease transmission. Chapter assessment Apply basics of biotechnology and genetic engineering, to provide an understanding of the importance of molecular methods in the construction of microbial products for scientific, medical, and industrial uses. Chapter assessment Apply microbiology laboratory safety rules and maintain lab equipment and lab environment in accordance with those rules. Chapter assessment Perform standard microbiological lab techniques including, use of the bright

5 field microscope, aseptic technique, smear preparation and staining, inoculation/streaking techniques, media preparation, serial dilutions, and incubation protocols. Chapter assessment Instructor's Requirements There will be an assessment on each chapter, three 2-hour examinations, one 1-hour comprehensive departmental final examination and one laboratory examination. Computer literacy is necessary as students will be required to obtain course materials from the HCC Eagle Online website. There will be no make-up examination unless there is a genuine excuse supported by acceptable documentation in which case the student must make up the missed examination within one week of the date of the examination. Chapter assessments must be submitted on due dates. There will be no time extension on any chapter assessment due date under any circumstance. Laboratory reports not submitted on the due date will attract a penalty of 2 points if submitted on the next day after the due date and an additional 1 point for each day thereafter. The penalty points shall be deducted from the laboratory reports grade. To minimize distractions in class and laboratory, students must turn off their cell phones or put them in the vibrate mode. Eating and drinking are not allowed in the classroom and laboratory. Any form of foul language is strictly prohibited. Please allow at least 48 hours for responses to your phone calls and s. Students must adhere to the rules and policies in the Houston Community College student handbook. Program/Discipline Requirements: If applicable HCC Grading Scale: Comprehensive Departmental Final Examination A = points per semester hour B = 89-80: 3 points per semester hour C = 79-70: 2 points per semester hour D = 69-60: 1 point per semester hour 59 and below = F 0 points per semester hour FX (Failure due to non-attendance) 0 points per semester hour IP (In Progress) 0 points per semester hour W (Withdrawn) 0 points per semester hour I (Incomplete) 0 points per semester hour AUD (Audit) 0 points per semester hour IP (In Progress) is given only in certain developmental courses. The student must reenroll to receive credit. COM (Completed) is given in non-credit and continuing education courses. FINAL GRADE OF FX: Students who stop attending class and do not withdraw themselves prior to the withdrawal deadline may either be dropped by their professor for excessive absences or be assigned the final grade of "FX" at the end of the

6 semester. Students who stop attending classes will receive a grade of "FX", compared to an earned grade of "F" which is due to poor performance. Logging into a DE course without active participation is seen as non-attending. Please note that HCC will not disperse financial aid funding for students who have never attended class. Students who receive financial aid but fail to attend class will be reported to the Department of Education and may have to pay back their aid. A grade of "FX" is treated exactly the same as a grade of "F" in terms of GPA, probation, suspension, and satisfactory academic progress. To compute grade point average (GPA), divide the total grade points by the total number of semester hours attempted. The grades "IP," "COM" and "I" do not affect GPA. Health Sciences Programs Grading Scales may differ from the approved HCC Grading Scale. For Health Sciences Programs Grading Scales, see the "Program Discipline Requirements" section of the Program's syllabi. Instructor Grading Criteria Chapter assessments 20% Examination 1 (chapters 1 5) 15% Examination 2 (chapters 6 12) 15% Examination 3 (chapters 13 17) 15% Departmental Final Examination (chapters 1 17) 5% Laboratory examination 10% Laboratory reports 20% Instructional Materials Textbook: Foundations In Microbiology, 8 th Edition, Kathleen Park Talaro McGraw Hill LAB Manual: Lab Manual For Microbiology 2420, 5th Edition.Edited by: Donna Wiersema & Permila Sen, 2007 HCC Policy Statement: Access Student Services Policies on their Web site: HCC ADA Statement: Any student with a documented disability (e.g. physical, learning, psychiatric, vision, hearing, etc.) who needs to arrange reasonable accommodations must contact the Disability Services Office at the Northeast College at the beginning of each semester. Instructors are authorized to provide only the accommodations requested by the Disability Support Services Office. If you have any special needs or disabilities that may affect your ability to succeed in college classes or participate in any college programs or activities, please contact the DSS office for assistance. ADA counselor contact information:

7 Kim Ingram (713) Room Northline campus 8001 Fulton Street, Houston, TX HCC Policy Statement: Academic Honesty Students are responsible for conducting themselves with honor and integrity in fulfilling course requirements. Disciplinary proceedings may be initiated by the college system against a student accused of scholastic dishonesty. Penalties can include a grade of "0" or "F" on the particular assignment, failure in the course, academic probation, or even dismissal from the college. Scholastic dishonesty includes, but is not limited to, cheating on a test, plagiarism, and collusion. HCC Policy Statement: Student attendance, Repeaters, withdrawal deadline Attendance Students are expected to attend classes and laboratory sessions regularly. Instructors check class attendance daily. It is the student s responsibility to always sign his/her name on the attendance sheet. 2 points shall be deducted from the final grade for each laboratory or class session missed. A student may be dropped from a course for excessive absences after the student has accumulated absences in excess of 12.5% of the hours of instruction (including lecture and laboratory time). Note that 12.5% is approximately 4 classes or laboratory sessions for a 4-semester hour course. Students are responsible for materials covered during their absences, and it is the student's responsibility to consult with instructors for make-up assignments. Habitual tardiness will not be tolerated. Students are expected to be in attendance for the entirety of the scheduled class and are responsible for completing assignments scheduled during their absence/s. It is the student s responsibility to amend their professional/personal schedule to meet the provided class schedule. Repeaters Students who repeat a course for a third or more times may soon face significant tuition/fee increases at HCC and other Texas public colleges and universities. Please ask your instructor / counselor about opportunities for tutoring / other assistance prior to considering course withdrawal or if you are not receiving passing grades. EGLS3 -- Evaluation for Greater Learning Student Survey Withdrawals Withdrawal from the course after the official day of record (see current catalog) will result in a final grade of W on the student transcript and no credit will be awarded. It is the student s responsibility to initiate and complete a request for withdrawal from any course. Students will be required to formally request a drop from their instructors prior to the administrative drop date deadline (Monday, March 31 st 2014). Abandoning the course or failing to formally drop, will result in a grade being given based on the work completed for the entire course (including missed exams). At Houston Community College, professors believe that thoughtful student feedback is necessary to improve teaching and learning. During a designated time near the

8 System end of the term, you will be asked to answer a short online survey of research-based questions related to instruction. The anonymous results of the survey will be made available to your professors and department chairs for continual improvement of instruction. Look for the survey as part of the Houston Community College Student System online near the end of the term. Distance Education and/or Continuing Education Policies Access DE Policies on their Web site: Access CE Policies on their Web site: LECTURE TOPICS Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Chapter 6 Chapter 7 Chapter 8 Chapter 9 Chapter 10 Chapter 11 Chapter 12 Chapter 13 Chapter 14 The Main Theme of Microbiology The Chemistry of Biology Tools of the Laboratory: The methods for Studying Microorganisms A Survey of Prokaryotic Cells and Microorganisms A Survey of Eukaryotic Cells and Microorganisms An Introduction to Viruses Elements of Microbial Nutrition, Ecology, and Growth An Introduction to Microbial Metabolism: The Chemical Crossroads of Life Microbial Genetics Genetic Engineering: A Revolution in Molecular Biology Physical and Chemical Agents for Microbial Control Drugs, Microbes, Host The Elements of Chemotherapy Microbe Human Interactions: Infection and Disease Host defenses: Overview and Nonspecific Defenses

9 Chapter 15 Chapter 16 Chapter 17 Adaptive, Specific Immunity and Immunization Disorders in Immunity Diagnosing Infections LABORATORY EXERCISES Exercise 1 Exercise 2 Exercise 3 Exercise 4 Exercise 5 Exercise 6 Exercise 7 Exercise 8 Exercise 9 Exercise 10 Exercise 11 Exercise 12 Exercise 13 Exercise 14 Exercise 15 Exercise 16 Exercise 17 Exercise 18 Exercise 19 Exercise 20 Exercise 21 Care and Use of the microscope Smear Preparation, Simple Staining and Bacterial Morphology Negative Staining Isolation of Bacteria Using the Streak Plate Method Gram Staining Acid-Fast Staining Bacterial Endospores Bacterial Capsules Bacterial Flagella and Motility Testing Selective, Differential, and Enriched Media Gas Requirements for the Growth of Bacteria Counting Bacteria in Milk Using the Pour-Plate Method Control of Microbial Populations:Effects of Heat Control of Microbial Populations:Effects of Chemicals Carbohydrate Fermentation by Bacteria Triple Sugar Iron (TSI) Fermentation Test The IMViC Biochemical Tests Rapid and Miniaturized Methods for Identification of Bacteria Bacteriophages Immune System: White Blood Cells Antibiotic Sensitivity Testing: Kirby-Bauer Method

10 EXAMINATIONS, CHAPTERS COVERED, AND DATES EXAMINATION 1 (Chapters 1 5) Thursday Feb 20 EXAMINATION 2 (Chapters 6 12) Thursday Mar 27 EXAMINATION 3 (Chapters 13 17) Thursday Apr 24 DEPARTMENTAL EXAMINATION (Chapters 1 17) Thursday May 1 LABORATORY EXAMINATION Thursday Apr 24 SESSION 1 SUGGESTED ACTIVITY Chapter 1 - The Main Themes of Microbiology Chapter 2 - The Chemistry of Biology Chapter 3 - Tools of the Laboratory Distribute course syllabus and materials OBJECTIVES 1. To discuss the benefits and risks of microorganisms in everyday life. 2. To understand the scope and significance of studying microbiology, including the kinds of 3. To learn the general characteristics of the different types of microbes. 4. To glimpse the historical foundations of microbiology. 5. To discover the basis for and significance of organizing, classifying and naming 6. To discuss strategies for studying microbes. 7. To study various methods for identification and classification of microbes including macroscopic colony observation, staining techniques, and biochemical reactions. 8. To learn basic principles of light and electron microscopy. 9. To Describe the types of media used for cultivation of microbes. SESSION 2 OBJECTIVES Laboratory Exercise 1 - Care and Use of the Microscope Departmental Laboratory Safety Rules and Regulations Each laboratory exercise has specific objectives to be discussed with the students. SESSION 3 OBJECTIVES Chapter 4 - A Survey of Prokaryotic Cells and Microorganisms

11 1. To describe the general external and internal cellular structures of a procaryotic cell. 2. To describe the various shapes, arrangements, and dimensions of bacterial cells. 3. To introduce more sophisticated bacterial classification and identification methods including: serological analysis, genetic and molecular analysis, DNA sequence analysis, and rrna analysis. 4. To survey groups of bacteria bearing unusual characteristics. SESSION 4 Laboratory Exercise 2 - Simple Staining and Bacterial Morphology Laboratory Exercise 3 - Negative Staining SESSION 5 Chapter 5 - A Survey of Eukaryotic Cells and Microorganisms OBJECTIVES 1. To theorize as to the evolution of the eucaryotic cell. 2. To review eukaryotic cell structure and organelle function. 3. To discuss Kingdom Mycetae(Fungi) and fungal diseases that affect man. 4. To discuss important protozoal diseases of man. 5. To survey the multitude and diversity among the eucaryotic microbial world, with a focus on the different styles of nutrition and habitat, locomotion, life cycles, and human disease. SESSION 6 Laboratory Exercise 4 - Isolation of Bacteria Using the Streak Plate Method Laboratory Exercise 5 - Gram Staining EXAMINATION 1- Chapters 1, 3, 4, 5 (Thursday, February 20th, 2014) SESSION 7 Chapter 6 - An Introduction to the Viruses OBJECTIVES 1. To understand the virus as an obligate intracellular parasite. 2. To study the general structures and components of viruses. 3. To classify viruses into groups. 4. To learn different modes and host ranges of viral replication. 5. To describe cultivation of animal viruses. 6. To discuss the impact of viruses human health and potential treatments. SESSION 8 Laboratory Exercise 6 - Acid-Fast Staining Laboratory Exercise 7- Bacterial Spores

12 SESSION 9 Laboratory Exercise 8 - Bacterial Capsules Laboratory Exercise 9 - Bacterial Flagella and Motility Testing SESSION 10 Chapter 7 - Elements of Microbial Nutrition, Ecology and Growth OBJECTIVES 1. To learn the many organic and inorganic nutritional requirements of microbes. 2. To understand various strategies that different microbes use to obtain nutrients. 3. To discuss the environmental factors that influence growth and survival of 4. To describe the bacterial growth curve. 5. To discuss methods for analyzing microbial growth. SESSION 11 Chapter 8 - An Introduction to Metabolism: The Chemical Crossroads of Life Laboratory Exercise 10 - Selective, Differential, and Enriched Media Gas Requirements for the Growth of Bacteria SESSION 12 Chapter 9 - Microbial Genetics OBJECTIVES 1. To study the basics of heredity, genetics and genes. 2. To learn how DNA is transcribed into RNA and RNA is translated into protein. 3. To discuss how protein synthesis and metabolism are controlled in 4. To understand how various changes and exchanges in microbial DNA can arise. SESSION 13 Laboratory Exercise 12 - Counting Bacterial in Milk Using Pour-Plate Method SESSION 14 Chapter 10 - Genetic Engineering: A Revolution in Molecular Biology OBJECTIVES 1. To survey the basic elements and applications of Genetic Engineering. 2. To learn the tools and techniques of Genetic Engineering. 3. To describe the methods for gene cloning and recombination. 4. To discuss the benefits of the products of Genetic Engineering. 5. To learn how scientists create recombinant plants and animals and

13 how these organisms can benefit us. 6. To introduce ways in which DNA can be exploited as medicine. 7. To survey methods for analyzing the makeup and mechanisms of the human body. SESSION 15 Chapter 11 - Physical and Chemical Control of Microbes SESSION 16 Chapter 12 - Drugs, Microbes, Host -- The Elements of Chemotherapy OBJECTIVES 1. To understand how the growth of microorganisms is controlled, including what is microbial death and the modes of action of antimicrobial agents. 2. To survey physical methods of controlling microbes. 3. To discuss chemical methods of controlling microbes. 4. To study the principles of antimicrobial therapy. 5. To describe the interactions between drug and microbe. 6. To survey the various groups of antimicrobial drugs. 7. To understand the characteristics of host/drug interactions. 8. To learn the considerations in the selection of an antimicrobial agent. SESSION 17 Laboratory Exercise 13 - Control of Microbial Populations: Effect of Heat SESSION 18 Laboratory Exercise 14 - Control of Microbial Populations: Effects of Chemicals Laboratory Exercise 21 - Antibiotic Sensitivity Testing: Kirby-Bauer Method EXAMINATION 2 Chapters 6, 7, 8, 11,12 (Thursday, March 27th 2014) SESSION 19 Chapter 13 - Microbe-Human Interactions: Infection and Disease OBJECTIVES 1. To survey the human as host to resident flora. 2. To understand the progression of an infection from route of entry, to mechanisms of invasion and establishment, to microbial resistance factors, to signs and symptoms of human disease, to route of exit. 3. To introduce principles of epidemiology. 4. To review Koch s Postulates. SESSION 20

14 Laboratory Exercise 15 - Carbohydrate Fermentation by Bacteria Laboratory Exercise 16 - Triple Sugar Iron (TSI) Fermentation Test SESSION 21 Chapter 14 - The Nature of Host Defenses OBJECTIVES 1. To realize the first line of host defense against microbial infection. 2. To understand the molecular basis of the immune response including surveillance, recognition, and destruction. 3. To survey the body systems involved in the immune response. 4. To discover the non-specific immune reactions of the body in response to microbial invasion. 5. To introduce the specific immune system. SESSION 22 Laboratory Exercise 17 - The IMViC Biochemical Tests SESSION 23 T0PIC TO BE COVERED Chapter 15 - Adaptive, Specific Immunity and Immunization OBJECTIVES 1. To realize the dual nature of specific immunity: humoral and cellmediated. 2. To understand underlying concepts of specific immunity, including the recognition of self and non-self, and the source of diversity and specificity. 3. To learn the complex involvement and responsibilities of lymphocytes in humoral and cell-mediated immunity. SESSION 24 Laboratory Exercise 18 - Rapid and Miniaturized Methods for Identification of Bacteria Laboratory Exercise 19 - Bacteriophages SESSION 25 Chapter 16 - Disorders in Immunity OBJECTIVES 1. To survey the practical applications of immunological function. These include Methods and principles of immunization Principles of vaccine preparation Strategies for new vaccines Exploitation of immunological responses for obtaining clinical and research data 2. To introduce microbial diseases. 3. To describe how an infectious agent is isolated from the patient,

15 cultivated, and identified as the causative agent. SESSION 26 Chapter 17 - Disorders in Immunity Laboratory Exercise 20 - Immune System: White Blood Cells SESSION 27 EXAMINATION 3 - Chapters 12, 13, 14, 15, 16, 17 (Thursday April 24th, 2014) LABORATORY EXAMINATION (Thursday April 24, 2014) DEPARTMENTAL FINAL EXAMINATION 3 - Chapters 1-17 (Thursday May 1st, 2014)