A 6( mrna

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1 Name: Row: Date: Period: Protein Synthesis Worksiieet Directions: 1^* Fill in tlie complimentai^^ DN strand using DN base pairing rales. Fill in the correct mrn bases by transcribing the bottom DN code. 3"^ ranslate the nirn codons andfindthe correct amino acid using the Codon able 4^*" Write ill the amino acid and the conect anti-codon the trn molecule. 5* he answer to the questions about protein synthesis below the amino acids. d) () () (s) d) (i) (i) ( ) d) (i) d) ^ ( ) d) 4 OOOOOOOOOOOOOOO l l l l l l l l l l l l l l l DN 6( mrn 5. mrn is synthesized in traaslation or ti^scription? mino 6. mrn lias codons or anti-codobs? ) ) i) ona 7 OOOOOOOOOOOOOOO ""'^ ' I I ^RN VV9V9 trn mino

2 n O O O O O O O O O O O O O O O " " ' ^ l l l l l l l l l l l l l l l i2.(i666666(!)66 WRN 13.9 Q trn 14. mino 15. i or 3 codons equal one aniino acid? 16. trn brings amino acids to the nucleus or ribosome? 17. polypeptide is a sequence of proteins or amino acids? 18. trn has codons or anti-codons? 19. frn tiansfers amino acids during translation or transcription? 20. Ribosomes are the site where translation or transcription takes place? DN d) ^RN 23.9 O trn 24. mino

3 ranscription Worl<slieet 1. Wliat are the bases in DN? 2. How do the bases pair up? 3. What are the bases in RN? What RN nitrogen bases match with the following DN nitrogen bases? C 4. If this is a sequence of one DN strand, what is the complementary sequence of the other DN strand? CC 5. What in RN sequence would bond with DN strand from question 5? CC 6. hree RN bases make a 7. What does a codon code for? 8. Ifyou have 1500 base pairs, how many amino acids are there? 9. he shape of a DN molecule is

4 10. What amino acids are made from these bases? (See chart) C UUC U CC 11. What do proteins do for us? 12. Where are proteins made in the cell (organelle)? Imagine you have the following strands of DN. o through the process of transcription and translation and build the correct protein. DN mrn mino cid C C C

5 SVHS LB BIOLOSY ( ) NME; PERIOC: ) If the substitution occurred to the 6**^ base on the template side, such that the cytosine was changed to a thymine, would the protein change? Why? 6) Summarize the roles of the following enzymes that play a role in DN functions? DN RNSCRIPION & RNSLION WORKSHEE a. Helicase b. DN polymerase ^ 1) Each DN molecule has two sides, one is called the tempiate from which the mrn is constructed by RN polymerase, and the other fs the coding side which codes for Q protein. If the template side of a DN molecule is the sequence shown below, what wili the coding side base sequence be? (Show the proper number of hydrogen bonds connecting the bases) c. RN polymerase 7) Describe the location of each process listed betow and what then name the product of each process. a. ranscription, P^jji^g I II I I M I I I t I 11 I I I I M I I li I I I b. ranslation,,, mlate C C C C ( 3 C CCC C ^ M M M I I I M M 1 I M I I I II I I I i I 8) Describe the role of the foiiowing base(s) on the DN molecule. a. Codon 2) If the template strand from above is used, what wifl the resulting mrn molecule base sequence be? Write the base sequence for mrn above the bases of the DN molecule. Diagram a RN nucleotide (197) In the box provided. a. Promoter b. erminotion codon 9) List the start and stop codons found on RN. a. Start codon b. Stop codons emplate CCCCCCC<3CC 1 I M I I M I M II I I i I i I M M I I M 10) If the DN coding strand is &&C&&. what would the amino acid sequence be? 3) Using the mrn codon list (Page 207) for amino acids, determine the amino acid sequence for the mrn sequence above. 11) If the above sequence was struck by radiation and the last "" in "S" was changed to an "", how would the amino acid sequence be changed? 4) If a mutation occurred to the fourth base in the template side, thymine, and a substitution occurred such that a guanine replaced the thymine, would the protein be changed? If so, how? 12) If the last base in the underlined sequence was changed by mutation to a "C", how would the protein be changed? 13) If the 4^^ base in the sequence was deleted by radiation (mutation), how would the sequence be changed?

6 ranscription: mrn in Eukaryotes Page 1 of 1 RN-coding sequencs DN Pre-mRN Promoter Cap 5'.' t Leader ranscription: Exon 1 ntron Eron^J ntron Exon railer erminator PolyW tail introns removed Protein-coding sequence mrn l-''' ranslation Polypeptide Most eukaryotic protein-coding genes contain segments called introns, which break up the amino acid coding sequence into segments called exons. he transcript of these genes is the pre-mrn (precursor-mrn), he pre-mrn is processed in the nucleus to remove the introns and splice the exons together into a translatable mrn. hat mrn exits the nucleus and is translated in the cytoplasm. 11/3/2011