Schedule. Panel Discussion (30 minutes)
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1 Schedule Presentations (60 minutes) John Perkins (DSM) Lignocellulose biomass to ethanol Michael Leavell (Amyris) HTS and omics platform Jean-Paul Leonetti (Deinove SA) Deinococcus-based technology Hans van der Pol (Corbion/Purac) Bio-based products portfolio Panel Discussion (30 minutes)
2 Bright Science at DSM: Development of a Commercial Lignocellulosic Biomass-to-Ethanol Process John Perkins Principal Scientist December 11, 2013 BIO Pacific Rim Summit San Diego, CA
3 Safe harbor statement This presentation may contain forward-looking statements with respect to DSM s future (financial) performance and position. Such statements are based on current expectations, estimates and projections of DSM and information currently available to the company. DSM cautions readers that such statements involve certain risks and uncertainties that are difficult to predict and therefore it should be understood that many factors can cause actual performance and position to differ materially from these statements. DSM has no obligation to update the statements contained in this presentation, unless required by law. The English language version of this document is leading. A more comprehensive discussion of the risk factors affecting DSM s business can be found in the company s latest Annual Report, which can be found on the company's corporate website,
4 DSM the Life Sciences and Material Sciences Company Global science-based company active in health, nutrition and materials 23,500 employees deliver annual net sales of over 9 billion 10% Workforce in R&D with budget of 490 million No. 1 or 2 in Dow Jones Sustainability Index from 2004 to 2013
5 2nd International Polysaccharide Conference, Aug 29 -Sep 2, 2011 DSM s Biotechnology Platform Centuries of continuous innovations CLASSICAL BIOTECH (19 th century) MODERN BIOTECH (20 th century) INDUSTRIAL BIOTECH (21 st century) Yeast Ethanol Yeast extracts Vitamins Penicillin Enzymes Citric acid Recombinant enzymes (e.g. Chymosin) Metabolic engineering (e.g. Vitamin B2,Cephalexin) Biocatalysis (e.g. Pharma intermediates) Cell culture (e.g. Per C6TM cell line) New feedstocks New/improved biomass conversion technologies New platform chemicals New biomaterials New biofuels
6 DSM Breakthrough in Yeast & Enzyme Technology ( ) R&D Piloting Thermostable cellulase mix discovered Costs reduced by factor of 17 Efficient C5 yeast conversion enabled Performance of yeast and enzymes confirmed Supported by the European Seventh Framework Programme
7 2nd International Polysaccharide Conference, Aug 29 -Sep 2, 2011 DSM C5 Yeast Strain Performance Proven at Industrial Scale
8 Next Breakthrough in Lowest Cost Ethanol Package Requires Integrated View Enzymes Pretreatment CBH1 BG EG-IV Interdependent! CBH2 Yeast EG-1 EtOH Cost Biomass Synergy!!
9 2nd International Polysaccharide Conference, Aug 29 -Sep 2, 2011 C5 Yeast Strains With Improved Robustness Feedstock with high inhibitor concentration. Strain I Strain II Yield: 77% Yield: 85% A B Pitch: 1 g/l; NREL dilute acid & enzymatically hydrolyzed corn stover: 17% dw; 5 g/l Hac; ph: 5.0 Yield on ethanol produced from total sugars (C5 & C6) increased by 8% Increased xylose consumption
10 Increasing yield by reducing by-product formation and conversion of inhibitors gpd1 gpd2 AAcDH OE GLY DHAP + NADH no glycerol production reduction of inhibitory HAc increased ethanol yield (HAc to ethanol) DSM has an exclusive license to this technology
11 Pathway Optimization All 1280 pathways were assembled from the separate cassettes 28 separate cassettes Completed pathways in MTP format Transformation host strain Colony picking Screening in liquid media NMR analysis Statistics 10
12 2nd International Polysaccharide Conference, Aug 29 -Sep 2, 2011 Glycerol/acetic acid technology Benefits in relation to enzyme cost RN1016 RN1016 Next strain Glycerol KO+ acetic acid External glycerol addition Up to 28% cost reduction Next strain Glycerol KO+ acetic acid External Glycerol addition Enzyme Cost Contribution does not take into account additional cost for procurement of glycerol 11
13 Optimized Thermostable Enzyme Cocktail Enzymes CBH1 BG EG-IV CBH2 Yeast EG-1
14 Enzyme Cost Reduction Cost of Enzyme $/gallon Ethanol 4Q08 2Q09 4Q09 2Q10 4Q10 2Q11 2Q13
15 Summary CBH1 Enzymes CBH2 BG Yeast Corn bio-ethanol EG-1 EG-IV We continue to innovate together with our partners Interdependent! POET Pilot Plant $4.13 Cellulosic bio-ethanol EtOH Cost POET Pilot Plant < $ 3 Pretreatment Biomass Future
16 Thank you
17 Back-up Slides
18 Oil consumption Our vision: From land to fossils and back to land 1 billion people 9 billion people Living off the land A brief moment in history Living off the land
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