BIO PACIFIC RIM SUMMIT
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1 Dec 9 th, 2013 Nagib M. WARD, Executive VP BIO PACIFIC RIM SUMMIT
2 Number of publications Number of patents DEINOCOCCUS A NEW SCIENTIFIC AND TECHNOLOGICAL OPPORTUNITY NUMBER OF PUBLICATIONS PER GENUS NUMBER OF PATENTS PER GENUS Escherichia coli Microorganisms Microorganisms Saccharomyces cerevisiae Deinococcus Deinococcus geothermalis Deinove holds the largest collection of Deinococci in the world (6,000 strains) A proprietary technological platform (16 patents) with validation through a POC (DEINOL) The world pioneer in deinotechnologies and their IP. 2
3 DEINOVE DEINOCOCCUS GENUS D. radiodurans discovered in 1956, by accident. Pr. Miroslav Radman: DNA repair research. Industrial tool: Deinove creation in 2006.
4 DEINOVE CORPORATION KEY DATES Deinobank (25 strains) Proprietary technological platform Initiation of DEINOBIOTICS program Initiation of DEINOCHEM program Deinobank (6 000 strains) Initiation of DEINOL program DEINOL EC2 (POC) THANAPLAST program DEINOL EC1 DEINOBIOTICS SAS Inception, co-founders Pr Miroslav RADMAN Dr Philippe POULETTY OSEO funding ( 6 m) Jacques BITON CEO OSEO funding ( 1.57 m) IPO ( 12 m) Partnership with TEREOS Partnership with VTT OSEO funding ( 1.4 m) Emmanuel PETIOT CEO Nagib WARD Exec VP OSEO funding ( 1.15 m) Market Cap: 53 m 30 FTE Staff
5 DEINOVE 6000 STRAINS LIBRARY, MANY UNSUAL Diverse environments have been targeted unusual selection by irradiation unusual carbon sources Deinococcus library (1800 strains) AND diverse library of original strains (4200) All strains collected according to the international laws (CBD) Source of original new biocatalysts 5
6 DEINOVE RESEARCH CENTER BIOPÔLE EUROMÉDECINE IN MONTPELLIER, FRANCE 1000 m EMPLOYEES 6
7 DEINOVE SSCF PROCESS 7
8 DEINOVE INDUSTRIAL ADVANTAGES Operating at 47-52C allows maximizing enzymes at their biochemical optimum, minimizing doses. CBP: enzymes are excreted in solutions to boost digestion, accelerate cofermentation Outstanding molecular biology tool to express specific sequences: Enzymes Metabolic Pathways An polyextremophile with remarkable resistance to harsh conditions or toxic substances. Vast IP opportunities for a largely unknown and underutilized bacterial strain. Metabolizes C3s but also coassimilates C6 and C5s. 8
9 DEINOVE PROCESS BENEFITS IN INDUSTRY Higher temperature (~50C): Inhibitors/Products resistance: Molecular Transformation: CBP/Enzyme release: IP Opportunities: Lower infection rates/risks Lower enzyme dose by leveraging biochemical optimums Allows utilizing cellulosic sugars Maximizes product yields Express proprietary sequences. Introduce new metabolic pathways Significantly affect conversion/hydrolysis costs Promote optimal process type: SSF Vastly unfiled IP field. 9
10 PIGMENTED BACTERIA BIODIVERSITY : PINK, ORANGE AND RED PIGMENTS Deinococcus ficus Deinococcus aquaticus Deinococcus daejeonensis Deinococcus marmoris Roseomonas aestuarii Rhodococcus corynebacterioides Kocuria turfanensis
11 DEINOVE BUSINESS MODEL 3: Feedstock 4: Process Benefits 1: Main Product 2: Co-Product
12 DEINOVE COMMERCIAL STRUCTURE Deinol Deinochem Deinobiotics Green Solvents Biofuels Renewable Chemicals Nutraceuticals Deinomediation ThanaPlast TM
13 DEINOVE MARKETS AND PRODUCTS ADVANCED CHEMICALS ETHANOL ADVANCED BIOFUELS RENEWABLE CHEMICALS & INTERMEDIATES HIGH-VALUE INTERMEDIATES BIODIGESTION SUGARS AND PREBIOTICS 13
14 THE DEINO WAY TO RENEWABLE CHEMICALS 14
15 DEINOCHEM OUR GREEN BIOTRANSFORMING PLATFORM Our biochemical platforms leverage Deinoccocus advantageous properties in industrial settings: The extreme robustness of the bacterial strains Their feedstock flexibility and usage efficiency The genetic modification efficiency and optimization of DEINOVE s strains These properties are key success factors for DEINOVE s targeted industrial applications, by: allowing a customization of the platforms for each commercial partner being independent of either the starting feedstock or pretreatment technology employed 15
16 DEINOCHEM BIOMASS VALUE GENERATION DEINOREDUCTION Nutraceuticals Solvents FERMENTATION PATH AcCoA HO HO O HO HO HO O ISOPRENOÏDS CH 3 HO H OH 3 C CH 2 CH 2 HO OH HO OH Geraniol Linalol Isoprene H 2 C CH 3 AcetoAcCoA MalonylCoA C3, C5 & C6 Sugars Conversion H 3 C CH 3 H 3 C CH 3 hydroxybutyrylcoa Cosmetic Speciality Chemicals crotonylcoa AMINO ACIDS DERIVED PATHWAYS H 3 C butyrylcoa OH O OH O CH 2 H 3 C O OH O HO O O H 3 C CH 3 H 3 C O OH NH 2 OH Isobutanol H 2 C H 2 N O Acrylate OH NH 2 Diaminobutane Butanol Solvents Paints Plastics Dispersants StyrEne H 3 C OH Butanol H 3 C CH 2 H 3 C Isobutene H 3 C CH 3 Renewable biochemicals 16
17 DEINOVE BIOTRANSFORMATION Specific molecular biology tools are necessary to biotransform Deinove s strains. Exact location genetic insertions, or deletions (KO). Numerous genetic cassettes can be expressed at high levels.
18 new sites in progress Available sites 0 Multi-targetting cassette Specific targetting cassette Deinococcus main chromosome
19 DEINOVE DEINOL CONVERSION RATES Green chemicals do not command price premiums in the market: Conversion rates and both products and feedstock prices are key! ADEME announced its support of Deinove s Terpenoids production ambition: $8.5MUSD in
20 DEINOCHEM MARKET OPPORTUNITIES Terpenoids: Linalool, Geraniol and B-Pinene Pathway Intermediates: Isopentene/isopentane Nutraceuticals: Green Chemicals: Prebiotics: Xylitol and Fucosylated Sugars Carotenoids Succinic Acid PDO Adipic Acid HMD 20
21 DEINOCHEM THE WAY TO ISOPRENOIDS The largest family of natural substances in the world: More than 22,000 isoprene compounds Many industrial applications: creams, fragrance, detergents, anti-cancer agents DEINOCHEM program Resources dedicated to the program (2012 launch/2013 acceleration) Promising early work from our laboratories Advanced discussions with many world leading industrials partners Looking for new production standards: Extraction from plants and chemical synthesis suffer from low yields and high production costs 21
22 DEINOCHEM HIGHER RESISTANCE TO ISOPRENOÏDS METABOLIZATION TEST (MTT) IC 50 (G/L) ISOPRENE GERANIOL LINALOOL b-pinene E. coli 7 0,6 4 91,7 S. cerevisiae 8 0,8 4 22,5 D. radiodurans R1 D. geothermalis MX6-1E D. geothermalis MX6-1C D. geothermalis MC2-2A D. geothermalis M36-7D 21 No Inhibition 25 22,5 12 7,8 5 ND 14 No Inhibition 7 ND 14 No Inhibition 7 ND 22 0,6 3 91,7 22
23 DEINOCHEM DEINOVE is now developing a brand new application platform named DEINOREDUCTION focused on the industrial bioreduction of various materials to produce high value renewable compounds. The first applications will be focused on the following opportunities: Xylitol PDO, Isopropanol and Butanol Fucose and fucosylated prebiotics 23
24 DEINOCHEM PRODUCTS OF INTEREST C6 derivatives, ideally from a cellulosic carbon source: Adipic Acid: >6BUSD Market C6 derivative, diamine for polyurethanes, nylon manufacturing. HMD: >4BUSD Market C6 derivative, dicarboxylic acid for polyesters, nylon manufacturing. Terpenoid pathway intermediates as solvents or synthetics: Isopentane: ~$2.5/Kg Isopentene: ~$3/Kg 24
25 DEINOCHEM HIGHER RESISTANCE TO SOLVENTS METABOLIZATION TEST (MTT) IC 50 (G/L) ETHANOL DECANOL SERINOL HEXANOL BUTANOL BUTANOL ACRYLIC HEXANOIC Gentle shaking Strong shaking ACID ACID E. coli ,2 S. cerevisiae 115 ND 93 ND ,3 D. radiodurans R D. geothermalis MX6-1E No inhibition No inhibition D. geothermalis MX6-1C No inhibition ND D. geothermalis MC2-2A No inhibition ND 1 ND D. geothermalis M36-7D ND ND
26 MERCI!!! THANK YOU!!! 26
27 CONTACTS Nagib M. WARD Vice President, Business Development CapTrust Tower, 4208 Six Forks Road, Suite 1000 Raleigh, NC USA (O): (M): (F): Jean-Paul LEONETTI VP R&D Tel : jean-paul.leonetti@deinove.com Emmanuel PETIOT CEO Tel : emmanuel.petiot@deinove.com 27
28 DISCUSSION SUPPORT
29 OUR PROPRIETARY TECHNOLOGICAL PLATFORM THE BEST OF GENETIC ENGINEERING AND BACTERIOLOGY COLLECTION ARCHIVING SELECTION OPTIMISATION SCALE UP Samples collection in extreme environment Library of cryopreserved strains Physiology High-throughput growth assays Metabolomic Functional tests Gene expression measurement Microbiology Genomic PCR Sequencing & Annotation Genetic & Metabolic Engineering Gene insertion Gene deletion Sequence optimization Fermentation engineering Optimization of culture & fermentation processes Industrial engineering Lab Assays Test & Integration DEINOBANK (6,000 clones) S1 S2 S3 LEAD DEINOCOCCUS POC CANDIDATE LAB PILOT INDUSTRIAL PILOT PRODUCTION 29
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