NF-kB Research Tools, Product Brochure

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1 NF-kB Research Tools, Product Brochure Interest in any of the products, request or order them at Bio-Connect. Bio-Connect B.V. T NL +31 (0) T BE +32 (0) Begonialaan 3a F NL +31 (0) F BE +32 (0) TE Huissen E info@bio-connect.nl The Netherlands W

2 w w w. i m g e n e x. c o m NF-κB R E S E A R C H T O O L S w w w. i m g e n e x. c o m

3 NF-kB Nuclear factor kb (NF-kB) is a nuclear transcription factor that regulates expression of a large number of genes that are critical for the regulation of apoptosis, viral replication, tumorigenesis, inflammation, and various autoimmune diseases. Composed of homo- and heterodimers of five members of the Rel family including NF-kB1(p50), NF-kB2 (p52), RelA, RelB, and c-rel (Rel), activation of NF-kB is thought to be part of a stress response as it is activated by a variety of stimuli including growth factors, cytokines, lymphokines, UV, pharmacological agents, and stress. In its inactive form, NF-kB is sequestered in the cytoplasm, bound by members of the IkB family of inhibitor proteins, which include IkBa, IkBb, IkBg, and IkBe. The various stimuli that activate NF-kB cause phosphorylation of IkB (through the IkB kinase complex consisting of at least three proteins: IKK1/a, IKK2/b and IKK3/g), which is followed by its ubiquitination and subsequent degradation. This results in the exposure of the nuclear localization signals (NLS) on NF-kB subunits and the subsequent translocation of the molecule to the nucleus. In the nucleus, NF-kB binds with the consensus sequence (5 GGGACTTTCC-3 ) of various genes, activating their transcription. A nuclear transcription factor that regulates expression of many critical genes. The study of NF-kB activation has never been more important than today. As its role in diverse biological processes, such as arthritis, asthma, heart disease, chronic inflammation, cancer and certain neuro-degenerative disorders, become clear, it is becoming apparent that more tools using evolving technology are needed. IMGENEX offers a broad portfolio of different NF-kB detection tools and technologies for measurement, quantitation, and elucidation of critical pathway components. These tools include both phospho and pan-specific mono- and polyclonal antibodies, novel non-radiometric readout assays for the quantification of IkBa phosphorylation levels and NF-kB/p65, as well as peptide decoy inhibitors. Also available is our expanding line of validated plasmid-based and synthetic RNA interference kits targeting specific NF-kB related proteins. NF-kB dimers are sequestered in the cytosol of unstimulated cells via non-covalent interactions with a class of inhibitor proteins, called IkBs. To date seven IkBs have been identified: IkB-alpha, IkB-beta, IkB-gamma, IkB-epsilon, BCL3, p100 and p105. All known IkBs contain multiple copies of a aa sequence, called ankyrin repeats which mediate the association between IkB and NF-kB dimers. The ankyrin repeats interact with a region in the RHD of the NF-kB proteins and by this mask their NLS and prevent nuclear translocation. Signals that induce NF-kB activity cause the phosphorylation of IkBs, their dissociation and subsequent degradation, thereby allowing activation of the NF-kB complex. Activated NF-kB complex translocates into the nucleus and binds DNA at kb-binding motifs such as 5-prime GGGRNNYYCC Application Key FA-A - Functional Assay (Activation) FA-Neut - Functional Assay (Neutralization) Flow-CS+Intra - Flow (Cell Surface) and Flow (Intracellular) IF/ICC - Immunofluorescence/Immunocytochemistry CM - Confocal Microscopy Flow-CS - Flow (Cell Surface) Flow-Intra - Flow (Intracellular) IHC-Fr - Immunohistochemistry (Frozen) IP - Immunoprecipitation IHC-P - Immunohistochemistry (Paraffin) WB - Western Blot PAP Peptide Affinity Purified NF-kB 1

4 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine) and induce gene expression. The degradation of IkB proteins that permits NF-KappaB molecules to move into the nucleus is also carried out by the proteasome but only after prior phosphorylation of IkB by the IKK (IkB Kinase Complex). The IKK is composed of three subunits: two, IKK-alpha (IKK1) and IKK-beta (IKK2), are bonafi de kinases, while the third, IKK-gamma (NEMO), has no catalytic activity but plays a critical regulatory role. IKK-alpha is the predominant IkB kinase. Phosphorylated IkB is recognized by Beta-TrCP, a component of the SCF (skp-1/cul/f box) ubiquitin ligase complex that mediates poly-ubiquitination of IkB and its subsequent proteasomal degradation. In contrast, IKK-alpha mediates the phosphorylationdependent processing of p100, resulting in the generation of p52. 2 T F E i n f i m g e n e x. c o m

5 NF-kB can be activated by exposure of cells to LPS (Lipopolysaccharides) or infl ammatory cytokines such as TNF (Tumour Necrosis Factor) or IL-1 (Interleukin-1), growth factors, lymphokines, oxidant-free radicals, inhaled particles, viral infection or expression of certain viral or bacterial gene products, UV irradiation, B or T-Cell activation, and by other physiological and non physiological stimuli. The most potent NF-kB activators are the proinfl ammatory cytokines IL-1 and TNF, which cause rapid phosphorylation of kbs at two sites within their N-terminal regulatory domain. TNF, which is the best-studied activator, binds to its receptor and recruits a protein called TRADD (TNF-Associated Receptor Death Domain). TRADD binds to the TRAF2 (TNF Receptor-Associated Factor-2) that recruits NIK (NF-kB-Inducible Kinase). Both IKK1 and IKK2 have canonical sequences that can be phosphorylated by the MAP (Mitogen Activated Protein) kinase NIK/MEKK1 and both kinases can independently phosphorylate IkB-alpha or IkB-beta. TRAF2 also interacts with A20, a zinc fi nger protein whose expression is induced by agents that activate NF-kB. A20 functions to block TRAF2-mediated NF-kB activation. A20 also inhibits TNF and IL-1 induced activation of NF-KappaB suggesting that it may act as a general inhibitor of NF-kB activation. CD40, another member of the TNF receptor family, can signal the induced processing of p100 to p52. The ligand for CD40, CD40L (CD154), is expressed on activated CD4-T cells, and when it engages CD40 in a T:B interaction, can induce B-Cell proliferation and differentiation. CD40 signaling induces p100 processing through NIK in the non-canonical pathway. LT-BetaR (Lymphotoxin Beta Receptor), which is critically important for the development and organization of lymphoid tissue also gives way to two separate pathways, one that activates the canonical NF-KappaB pathway and depends upon IKK-beta and IKK-gamma/NEMO and another that induces p100 processing dependent on NIK and IKK-alpha. References 1. Baldwin AS Jr. The NF-kappa B and I kappa B proteins: new discoveries and insights. Annu Rev Immunol. 1996;14: PMID: Vigo Heissmeyer, Daniel Krappmann, Eunice N. Hatada, and Claus Scheidereit. Shared Pathways of I B Kinase- Induced SCF TrCP-Mediated Ubiquitination and Degradation for the NF-kB Precursor p105 and I B. Molecular and Cellular Biology, February 2001, p , Vol. 21, No. 4 PMID: Baeuerle, P. A. I-kappa-B--NF-kappa-B structures: at the interface of infl ammation control. Cell 95: , PubMed ID : Joel L. Pomerantz and David Baltimore. Two pathways to NF-KappaB. Molecular Cell, Vol. 10, , October PMID: Fulvio D Acquisto, and Sankar Ghosh. PACT and PKR: Turning on NF-kB in the Absence of Virus. Science s STKE, 3 July PMID: Vishva Dixit and Tak. W. Mak. NF-KappaB signaling: Many Roads Lead To Madrid. Cell, Vol. 111, , November 27, 2000 PMID: Nanxin Li and Michael Karin. Ionizing radiation and short wavelength UV activate NF-KappaB through two distinct mechanisms. Proc. Natl. Acad. Sci. USA, Vol.95, pp , October PMID: Markus Müller, Alessandro Morotti, and Carola Ponzetto. Activation of NF- B Is Essential for Hepatocyte Growth Factor-Mediated Proliferation and Tubulogenesis. Molecular and Cellular Biology, February 2002, p , Vol. 22, No. 4 PMID: Michael Hinz, Daniel Krappmann, Alexandra Eichten, Andreas Heder, Claus Scheidereit, and Michael Strauss. NFkB Function in Growth Control: Regulation of Cyclin D1 Expression and G0/G1-to-S-Phase Transition. Molecular and Cellular Biology, April 1999, p , Vol. 19, No. 4 PMID: Application Key FA-A - Functional Assay (Activation) FA-Neut - Functional Assay (Neutralization) Flow-CS+Intra - Flow (Cell Surface) and Flow (Intracellular) IF/ICC - Immunofluorescence/Immunocytochemistry CM - Confocal Microscopy Flow-CS - Flow (Cell Surface) Flow-Intra - Flow (Intracellular) IHC-Fr - Immunohistochemistry (Frozen) IP - Immunoprecipitation IHC-P - Immunohistochemistry (Paraffin) WB - Western Blot PAP Peptide Affinity Purified NF-kB 3

antibodies NF-kB Description Cat No Format Clone Host Species Application Caspase-1 (IMG-5025) Formalin-fixed, paraffin-embedded section of human reactive lymph node stained for Caspase-1 expression

6 antibodies NF-kB Description Cat No Format Clone Host Species Application Caspase-1 (IMG-5025) Formalin-fixed, paraffin-embedded section of human reactive lymph node stained for Caspase-1 expression (IMG-5025) using a DAB chromogen and Hematoxylin counterstain. A20 IMG-161A Purified 59A426 Mouse H IP, WB ABIN-2 IMG-434 Purified N/A Rabbit H, M, R WB ACT1 IMG-284 Purified N/A Rabbit H WB AKT1 IMG-487 Purified N/A Rabbit H WB AKT1 IMG-5411 PAP N/A Rabbit H, M, R IHC-P, WB AKT1 (Ser473) IMG-187A Purified 104A282 Mouse H, M IP, WB AKT2 IMG-351 Ascites 95C Mouse H WB AKT2/3 IMG-421 Purified N/A Rabbit H WB AKT3 IMG-326 Purified 66C Mouse H, M, R WB AKT3 IMG-3389 PAP N/A Goat H WB AKT3 IMG-355 Purified N/A Rabbit H, M, R WB ARAP1 IMX-6347 Sera N/A Rabbit Multi-species ELISA ATM (Ser1981) IMG PAP N/A Rabbit H, M IHC-P Bcl-3 IMG-497 Purified N/A Rabbit H WB BLyS/BAFF IMG-247 Purified N/A Rabbit H, M WB BLyS/BAFF IMG-5610 Purified N/A Rabbit H, M, R WB BMI1 IMX-6345 Sera N/A Rabbit Multi-species ELISA CARMA1/ CARD11 IMX-5160 Sera N/A Rabbit H ELISA CARMA1/ CARD11 IMX-5161 Sera N/A Rabbit H ELISA Caspase-1 IMG-5025 Purified N/A Rabbit H IHC-P, WB Caspase-1 IMG-5028 Purified 14F468 Mouse H, M IHC-P, WB CIKS/Act1 IMG-563 Purified N/A Rabbit H, M, R WB CIKS/Act1 (NT) IMG-5447 PAP N/A Rabbit H WB CUEDC2 IMG-6279A Purified N/A Rabbit Multi-species WB CUEDC2 IMG-6299A Purified N/A Rabbit H IHC-P ECSIT (CT) IMG-5618 Purified N/A Rabbit H, M WB eif2a (Ab-51) IMG PAP N/A Rabbit H, M, R IHC-P, WB eif2a (Ser51) IMG PAP N/A Rabbit H, M, R IHC-P, WB GSK3a (Ser21) IMG PAP N/A Rabbit H, M, R IHC-P, WB GSK3b (Ab-216) IMG PAP N/A Rabbit H, M, R WB GSK3b (Tyr216) IMG PAP N/A Rabbit H, M, R IHC-P, WB HDAC2 (Ab-394) IMG PAP N/A Rabbit H, M, R IHC-P, WB HDAC-4 (Ser632) IMG PAP N/A Rabbit H, M, R WB HDAC5 (Ab-498) IMG PAP N/A Rabbit H, M, R IHC-P, WB HDAC5 (Ser498) IMG PAP N/A Rabbit H, M, R IHC-P, WB HDAC8 (Ab-39) IMG PAP N/A Rabbit H IHC-P, WB HDAC8 (Ser39) IMG PAP N/A Rabbit H, M, R IHC-P HNLF IMG-5112A Purified N/A Rabbit M, H, R WB IKAP IMG-4047 Purified N/A Rabbit H WB IkBa IMG-127A Purified 6A920 Mouse H, M Flow-Intra, IHC-P, IP, WB IkBa IMG-5016 Purified N/A Rabbit H WB IkBa IMG-5270A Purified N/A Rabbit H WB IkBa IMX-5142 Sera N/A Rabbit H ELISA IkBa IMG-127B Biotin 6A920 Mouse H, M ELISA IkBa (Ser32/Ser36) IMG-156A Purified 39A1413 Mouse Multi-species ELISA, IP, WB IMG-156B Biotin 39A1413 Mouse Multi-species ELISA IkBa (Tyr305) IMG-5783 PAP N/A Rabbit H, M, R WB IkBa (Tyr42) IMG-5782 PAP N/A Rabbit H, M, R IP, WB 4 T F E i n f i m g e n e x. c o m

Description Cat No Format Clone Host Species Application IkBa (Tyr42) IMX-5097 Purified N/A Rabbit Multi-species ELISA IkBa (cleavage specific) IMG-394 Purified 5D1623 Mouse H WB IkBe (Ser22)

7 Description Cat No Format Clone Host Species Application IkBa (Tyr42) IMX-5097 Purified N/A Rabbit Multi-species ELISA IkBa (cleavage specific) IMG-394 Purified 5D1623 Mouse H WB IkBe (Ser22) IMG PAP N/A Rabbit H, M IHC-P, WB IkBz (zeta) IMG-5098A Purified N/A Rabbit M WB IKKa (Ab-23) IMG PAP N/A Rabbit H, M, R IHC-P, WB IKKa (Thr23) IMG PAP N/A Rabbit H, M, R IHC-P, WB IKKa/IKK1 IMG-136A Purified 14A231 Mouse Multi-species Flow-Intra, IHC-P, IP, WB IKKa/IKK1 IMG-136AF488 Alexa Fluor A231 Mouse Multi-species Flow-Intra IKKa/IKK1 (C1) IMG-5477 PAP N/A Rabbit H WB IKKa/IKK1 (C2) IMG-5478 PAP N/A Rabbit H WB IKKb/IKK2 IMG-129A Purified 10AG2 Mouse H, M Flow-Intra, IP, WB IKKb/IKK2 IMG-129AF488 Alexa Fluor AG2 Mouse H, M Flow-Intra IKKb/IKK2 IMG-159A Purified 10A9B6 Mouse H, M IP, Flow-Intra, WB IKKb/IKK2 IMG-159AF488 Alexa Fluor A9B6 Mouse H, M Flow-Intra IKKb/IKK2 (C3) IMG-5570 PAP N/A Rabbit H IF/ICC, WB IKKe/IKKi IMG-309 Purified N/A Rabbit H WB IKKe/IKKi/TBK1 IMG-270A Purified 72B587 Mouse H, M, R Flow-Intra, WB IKKg/IKK3/NEMO IMG-287A Purified 46B844 Mouse H Flow-Intra, WB IKKg/IKK3/NEMO IMG-324A Purified 72C627 Mouse H, M WB IMG-324B Biotin 72C627 Mouse H, M ELISA IKKg/IKK3/NEMO IMG-4044 Purified N/A Rabbit H WB IKKg/IKK3/NEMO IMG-4045 Purified N/A Rabbit H WB IKKg/IKK3/NEMO IMG-5480 PAP N/A Rabbit H, M, R WB IRAK IMG-179 Purified N/A Rabbit H, M IP, WB IRAK-2 IMG-574 Purified N/A Rabbit H, M WB IRAK-2 (C2) IMG-5495 PAP N/A Rabbit M WB IRAK-4 IMG-441 Purified N/A Rabbit H, M IP, WB IRAK-4 IMG-5488 PAP N/A Rabbit H WB IRS-1 (Ser639) IMG PAP N/A Rabbit H, M, R IHC-P JNK2 IMG-586 Purified N/A Rabbit H WB kb-ras1 IMG-390 Purified 184C278 Mouse H WB Lck (Tyr393) IMG PAP N/A Rabbit H, M, R WB LST1 IMX-6348 Sera N/A Rabbit H ELISA MALT1 IMG-5110A Purified N/A Rabbit H WB MALT1 IMG-5745 Sera N/A Rabbit H IHC-Fr, IHC-P, IP, WB MSK1 (Ab-376) IMG PAP N/A Rabbit H, M, R WB MyD88 IMG-178 Purified N/A Rabbit H, M, R WB MyD88 IMG-3796 PAP N/A Goat H ELISA, IHC-P, WB MyD88 (CT) IMG-5499 PAP N/A Rabbit H, M, R WB MyD88 (IN) IMG-5579 PAP N/A Rabbit H IHC-P, WB NALP5 IMX-6336 Sera N/A Rabbit H ELISA NALP7 IMG-6357A PAP N/A Rabbit Multi-species WB NALP9 IMX-6343 Sera N/A Rabbit H ELISA NALP11 IMG-6443A Purified N/A Rabbit H IHC-P NALP11 IMX-6344 Sera N/A Rabbit Multi-species ELISA NFATc1 IMG-4073 Purified N/A Rabbit H WB NFATc1 IMG-4074 Purified N/A Rabbit H WB NFATc1 IMG-5101A Purified N/A Rabbit H WB NALP11 (IMG-6443A) Immunohistochemical analysis of NALP11 in formalin-fixed, paraffin-embedded human liver tissue using an isotype control (top) and IMG-6443A (bottom) at 5 ug/ml. NF-kB antibodies Application Key FA-A - Functional Assay (Activation) FA-Neut - Functional Assay (Neutralization) Flow-CS+Intra - Flow (Cell Surface) and Flow (Intracellular) IF/ICC - Immunofluorescence/Immunocytochemistry CM - Confocal Microscopy Flow-CS - Flow (Cell Surface) Flow-Intra - Flow (Intracellular) IHC-Fr - Immunohistochemistry (Frozen) IP - Immunoprecipitation IHC-P - Immunohistochemistry (Paraffin) WB - Western Blot PAP Peptide Affinity Purified NF-kB 5

antibodies NF-kB Description Cat No Format Clone Host Species Application TRAF6 (IMG-5766) Formalin-fixed, paraffinembedded tissue sections stained for TRAF6 using IMG-5766 at 1:2000.

8 antibodies NF-kB Description Cat No Format Clone Host Species Application TRAF6 (IMG-5766) Formalin-fixed, paraffinembedded tissue sections stained for TRAF6 using IMG-5766 at 1:2000. Hematoxylin-eosin counterstain. A, mouse lymph node. B, mouse bone marrow. NFATc2 IMG-4075 Purified N/A Rabbit H WB NF-kB (p50) IMG-5845A Purified N/A Rabbit Multi-species WB NF-kB (p50) IMG-5812A Purified 2J10D7 Mouse H IHC-P, WB NF-kB (p65) IMG-150A Purified 112A1021 Mouse H, M, R Flow-Intra, IHC-P, WB IMG-150B Biotin 112A1021 Mouse H, M, R ELISA NF-kB (p65) IMG-512 Purified N/A Rabbit H, M, R WB NF-kB (p65) IMG-5915A Purified N/A Rabbit Multi-species WB NF-kB (p65) IMG Purified N/A Rabbit Multi-species IHC-P NF-kB p65-nls IMG-6066A PAP N/A Rabbit Multi-species IF/ICC, WB NIBP IMX-5896 Sera N/A Rabbit Multi-species ELISA NIK IMG-443 Purified N/A Rabbit H WB NOD1/CARD4 IMG-5224A Sera N/A Rabbit H WB NOD1/CARD4 IMG-5739 Sera N/A Rabbit H IHC-Fr, IHC-P, IP, WB NOD2/CARD15 IMG PAP N/A Goat H WB NOD2/CARD15 IMG-5738 Sera N/A Rabbit H IHC-Fr, IHC-P, IP, WB NOD2/CARD15 IMX-5053 Sera N/A Rabbit Multi-species ELISA OPG IMG-103A Purified 98A1071 Mouse H IHC-P, WB P38 MAPK (Thr180) IMG PAP N/A Rabbit H, M, R IHC-P P38 MAPK (Tyr182) IMG PAP N/A Rabbit H, M, R IHC-P, WB p50 NLS IMX-6200 Sera N/A Rabbit Multi-species ELISA p62dok IMG-177 Purified N/A Rabbit H, M WB p65 (Ser276/281) IMX-5238 Sera N/A Rabbit H ELISA p65-cleaved IMX-5098 Purified N/A Rabbit H, M, R ELISA p90rsk (Ab-348) IMG PAP N/A Rabbit H, M, R WB p90rsk (Ab-352) IMG PAP N/A Rabbit H, M, R WB p90rsk (Thr348) IMG PAP N/A Rabbit H, M, R IHC-P, WB PKCb (Thr642) IMG PAP N/A Rabbit H, M, R IHC-P, WB PKCz (Ab-1427) IMG PAP N/A Rabbit H, M, R IHC-P PKCz (Thr410) IMG PAP N/A Rabbit H, M, R IHC-P PKR (Ab-446) IMG PAP N/A Rabbit H, M IHC-P, WB PKR (Ab-451) IMG PAP N/A Rabbit H, M IHC-P PKR (Thr446) IMG PAP N/A Rabbit H, M IHC-P, WB PKR (Thr451) IMG PAP N/A Rabbit H, M IHC-P PLCg2 (Ab-753) IMG PAP N/A Rabbit H, M, R WB PLCg2 (Tyr753) IMG PAP N/A Rabbit H, M, R WB PRKR IMG-5120A Purified N/A Rabbit H WB PRKR IMX-5200 Sera N/A Rabbit M ELISA PRKR IMX-5201 Sera N/A Rabbit M ELISA PRKR IMX-5202 Sera N/A Rabbit M ELISA RANK/TNF11aR IMG-128A Purified 9A725 Mouse H, M Flow-CS, IHC-P, WB IMG-128D PE 9A725 Mouse H, M Flow-CS+Intra RANK/TNF11aR IMG-325A Purified 64C Mouse H, M IP, WB RANK/TNF11aR IMG-5116A Purified N/A Rabbit M, H WB Relaxin Receptor 1 IMG-6184A Sera N/A Rabbit H IHC-P Relaxin Receptor 1 IMX-6184 Sera N/A Rabbit H ELISA RelB (Ab-552) IMG PAP N/A Rabbit H, M, R WB 6 T F E i n f i m g e n e x. c o m

Description Cat No Format Clone Host Species Application RelB (Ser552) IMG-90318-1 PAP N/A Rabbit H, M, R IHC-P, WB RIP IMG-5747 Sera N/A Rabbit H IHC-Fr, IHC-P, IP, WB SINTBAD/TBKBP1 IMG-6172A PAP

9 Description Cat No Format Clone Host Species Application RelB (Ser552) IMG PAP N/A Rabbit H, M, R IHC-P, WB RIP IMG-5747 Sera N/A Rabbit H IHC-Fr, IHC-P, IP, WB SINTBAD/TBKBP1 IMG-6172A PAP N/A Rabbit H, M, R IHC-P, WB SINTBAD/TBKBP1 IMX-6119 Sera N/A Rabbit Multi-species ELISA TAK1 binding protein 3 IMG-5900A Purified N/A Rabbit Dog, H, M WB TAK-1/MAP3K7 IMG-424 Purified N/A Rabbit H, M WB TAK-1/MAP3K7 (NT) IMG-5528 PAP N/A Rabbit H, M, R WB TBK1 (Ser172) IMX-5194 Sera N/A Rabbit H ELISA TBK1/NAK IMG-139A Purified 108A429 Mouse Multi-species WB TBK1/NAK (CT) IMG-5501 PAP N/A Rabbit H, M WB TRADD IMG-5756 Sera N/A Rabbit H IHC-Fr, IHC-P, IP, WB TRAF1 IMG-5757 Sera N/A Rabbit H IHC-Fr, IHC-P, IP, WB TRAF1 IMG-5758 Sera N/A Rabbit M IHC-Fr, IHC-P, IP, WB TRAF2 IMG-162 Purified 33A1293 Mouse H WB TRAF2 IMG-5759 Sera N/A Rabbit H, M, R IHC-Fr, IHC-P, IP, WB TRAF2 IMG-5760 Sera N/A Rabbit H IHC-Fr, IHC-P, IP, WB TRAF2 IMG-5761 Sera N/A Rabbit M IHC-Fr, IHC-P, IP, WB TRAF2 and TNIK IMG PAP N/A Rabbit H IF/ICC, IHC-P TRAF2 and TNIK IMG PAP N/A Rabbit H IHC-P TRAF3 IMG-535 Purified N/A Rabbit H, M WB TRAF3 IMG-5762 Sera N/A Rabbit H IHC-Fr, IHC-P, IP, WB TRAF3 IMG-5763 Sera N/A Rabbit Multi-species IHC-Fr, IHC-P, IP, WB TRAF4 IMG-5764 Sera N/A Rabbit Multi-species IHC-Fr, IHC-P, IP, WB TRAF5 IMG-163A Purified 55A219 Mouse H WB TRAF5 IMG-5765 Sera N/A Rabbit Multi-species IHC-Fr, IHC-P, IP, WB TRAF6 IMG-536 PAP N/A Rabbit H, M WB TRAF6 IMG-5766 Sera N/A Rabbit Multi-species IHC-Fr, IHC-P, IP, WB TRAF6 (CT) IMG-5654 Purified N/A Rabbit H, M, R IF/ICC, WB TRAF6BP/TAX1BP IMG-5035 Purified N/A Rabbit H WB TRAM IMG-5272A Purified N/A Rabbit Chimp, H, R. Mk IHC-P, WB Trance/RANKL IMG-133A Purified 12A668 Mouse H, M IHC-P, WB IMG-133B Biotin 12A668 Mouse H, M ELISA IMG-133E Azide-Free 12A668 Mouse H, M IHC-P, WB Trance/RANKL IMG-185A Purified 12A380 Mouse H, M Flow-CS, IHC-Fr, WB IMG-185D PE 12A380 Mouse H, M Flow-CS, IHC-Fr, WB Trance/RANKL IMG-5090A Purified N/A Rabbit M WB Trance/RANKL IMG-5108A Purified N/A Rabbit M WB TRIB3/SKIP3/SINK IMG-5118A Purified N/A Rabbit H WB Ubiquitin IMG-5021 Ascites Ubi-1 Mouse Multi-species IF/ICC, IHC-P, WB Ubiquitin IMG T.C. Sup. FPM1 Mouse H IHC-P Ubiquitin IMG Purified N/A Rabbit Multi-species IHC-P Ubiquitin IMG-5020 Sera N/A Rabbit Multi-species IF/ICC, WB Zap-70 (Ab-319) IMG PAP N/A Rabbit H, M, R IHC-P, WB Zap-70 (Ab-493) IMG PAP N/A Rabbit H, M, R IHC-P Zap-70 (Tyr319) IMG PAP N/A Rabbit H, M, R WB Zap-70 (Tyr493) IMG PAP N/A Rabbit H, M, R IHC-P Trance/RANKL (IMG-133A) Immunohistochemical staining of Trance/RANKL/ OPGL/ODF in formalin-fixed, paraffin-embedded human lymph node using IMG-133A at 5 ug/ml. Hematoxylineosin counterstain. NF-kB antibodies Application Key FA-A - Functional Assay (Activation) FA-Neut - Functional Assay (Neutralization) Flow-CS+Intra - Flow (Cell Surface) and Flow (Intracellular) IF/ICC - Immunofluorescence/Immunocytochemistry CM - Confocal Microscopy Flow-CS - Flow (Cell Surface) Flow-Intra - Flow (Intracellular) IHC-Fr - Immunohistochemistry (Frozen) IP - Immunoprecipitation IHC-P - Immunohistochemistry (Paraffin) WB - Western Blot PAP Peptide Affinity Purified NF-kB 7

NF-kB Pathway Inhibitory Peptides Cat No Description IMG-2000 IKKg NEMO Binding Domain (NBD) Inhibitory Peptide Set Functions as an IKKa/IKKb decoy by binding to IKKg NBD, thereby preventing

10 NF-kB Pathway Inhibitory Peptides Cat No Description IMG-2000 IKKg NEMO Binding Domain (NBD) Inhibitory Peptide Set Functions as an IKKa/IKKb decoy by binding to IKKg NBD, thereby preventing formation of the IKK complex. IMG-2001 NF-kB p65 (Ser276) Inhibitory Peptide Set Functions as a p65 decoy through phosphorylation of the Ser276 site on the peptide. IMG-2002 TRAF6 Inhibitory Peptide Set Functions as a TRAF6 decoy by binding to the T6DP motif of RANK, thereby preventing binding of RANK to TRAF6. IMG-2003 NF-kB p65 (Ser529/536) Inhibitory Peptide Set Functions as a p65 decoy through phosphorylation of the Ser529/536 sites on the peptide. IMG-2004 NF-kB p50 (NLS) Inhibitory Peptide Set Functions as a p50 decoy by blocking the intracellular recognition mechanism of p50 NLS. IMG-2005 MyD88 Homodimerization Inhibitory Peptide Set Functions as a decoy by binding to the MyD88 TIR domain. IMG-2006 TIRAP Inhibitory Peptide Set Functions as a TIRAP decoy by binding to TIR interacting domains on specific TLR receptors. 8 T F E i n f i m g e n e x. c o m

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NFκB Pathway Interest in any of the products, request or order them at Bio-Connect. Bio-Connect B.V. T NL +31 (0)26 326 44 50 T B +32 (0)2 503 03 48 Begonialaan 3a F NL +31 (0)26 326 44 51 F B +32 (0)2