Protein microarray and proteomics

Transcription

1 Chinese Bulletin of Life Sciences Vol. 17, No. 2 Apr., (2005) ; Q503;Q51 A Protein microarray and proteomics FEI Jia, MA Wen-Li*, ZHENG Wen-Ling (Institute of Molecular Biology, Southern Medical University, Guangzhou , China) Abstract: Protein microarray technology has become a crucial tool for large-scale and high-throughput biology. It allows fast, easy and parallel detection of thousands of addressable elements in a single experiment. This article reviewed protein chip's great potential in proteomics research. It has been applied to isolate, quantify protein and analyse protein-protein, protein-small molecule interaction. Protein chip combinated with mass spectrometry, biosensor technology expended its applicable scale. It also introduced the advance of proteome chip and alive chip in proteomics research. Protein microarray plays an important role in proteomics research. Key words: protein microarray; proteomics 1 [1] (two dimensional gel electrophoresis, 2DGE) (mass spectrometry, MS) [2] (Isoelectric focusing); SDS-PAGE (14 ) (14 ) * (12 )

2 20 80 Bjellqvist [3] 2DGE 2DGE (2) 1.2 (bio-mass spectrometry) (m/z) (3) DGE [7] (electrospray ionization ESI) (matrix-assisted laser desorption ionization MALDI ) [4~5] ( ); (2) DNA - 2 von Eggeling [8] Kodadek [6] ( ) 3.2 Mendoza [9] 36 8

3 coupled device) CCD(charge AIDA [10] Bacarese-Hamilton 5 IgG IgM 0.5pg 80% [11] Copeland 3.3 (2) (3) Wolf [16] (microfluidic networks, microfns) C (CRP) 10 1 CRP 30 ng/ml CRP (surface enhanced laser desorption/ionizationtime of flight-mass spectrometry, SELDI-TOF-MS) [12] Gray [17] [13] (Cdc28p) Cdc28p DNA 3.4 (Lab-on-chip) DNA [14] Chiem [15] 7.6cm.6cm (2) ( ( Western [19] - ); (3) ) [18] Davies

4 β (amyloid-β peptide A β) -Aβ( ) -Aβ Aβ SELDI-TOF-MS Aβ [20] Koopmann SELDI % 97% CA19-9(P<0.05) CA Pharmacia Biotech (biomolecular interaction analysis, BIA) (surface plasmon resonance,spr) (proteome chip) Zhu [22] Zhu [22] 4 DNA SPR BIA 3.8 ( ) MALDI-TOF-MS Uetz [23] 3.7 [21] 6000 (opening read frame ORF) Gal-4 DNA- RNA [24]

5 136, (2DGE) (MS) [1] Dove A. Proteomics: translating genomics into products? Nat Biotechnol, 1999, 17(3): 233~236 [2] O' Farrell P H. High resolution two-dimensional electrophoresis of proteins. J Biol Chem, 1975, 250(10): 4007~4021 [3] Bjellqvist B,Ek K Righetti P G et al. Isoelectric focusing in immobilized gradients: principle,methodology and some applications. J Biochem Biophys Methods, 1982, 6(4): 317~339 [4] Fenn J B Mann M, Meng C K, et al. Electrospray ionization for mass spectrometry of large biomolecules. Science, 1989, 246:64~71 [5] Karas M, Hillenkamp F. Laser desorption ionization of proteins with molecular masses exceeding daltons. Anal Chem, 1988, 60(20):2299~2301 [6] Kodadek T. Protein micriarray: prospects and problems. Chem Biol, 2001, 8(2):105~115 [7] Pennington S R, Dunn M J. : [M] ~176 [8] von Eggeling F, Davies H, Lomas L, et al. Tissue-specific microdissection coupled with Protein Chip array technologies: applications in cancer research. BioTechniques, 2000, 29(5): 1066~1070 [9] Mendoza L G, McQuary P, Mongan A, et al. High-throughput microarray-based enzyme-linked immunosorbent assay (). BioTechniques, 1999, 27(4):778~788 [10] Bacarese-Hamilton T, Mezzasoma L, Ardizzoni A,et al. Serodiagnosis of infectious diseases with antigen microarrays. J Appl Microbiol, 2004, :10~17 [11] Copeland S, Siddiqui J, Remick D.Direct comparison of traditional s and membrane protein arrays for detection / and quantification of human cytokines. J Immunol Methods, 2004, 284:99~106 [12] Cahill D J. Protein and antibody arrays and their medical application. J Immunol Methods, 2001, 250:81~91 [13] Gray N S, Wodicka L, Thunnissen WH,et al. Exploiting chemical libraries, structure, and genomics in the the search for kinase inhibitors. Science, 1998, 281: 533~538 [14] Emili A Q, Cagney G. Large-scale functional analysis using peptide or protein arrays. Nat Biotechnol, 2000, 18(4): 393~397 [15] Chiem N H, Harrison D J. Microchip systems for immunoassay: an integrated immunoreactor with electrophoretic separation for serum theophylline determination. Clin Chem, 1998, 44(3):591~598 [16] Wolf M, Juncker D, Michel B, et al.simultaneous detection of C-reactive protein and other cardiac markers in human plasma using micromosaic immunoassays and self-regulating microfluidic networks. Biosens Bioelectron, 2004, 19 (10): 1193~1202 [17] Merchant M, Weinberger S R. Recent advancements in surface-enhanced laser desorption/ ionization-time of flightmass spectrometry. Electrophoresis, 2000, 21(6):1164~1177 [18] MacBeath G, Schreiber S L. Printing proteins as microarray for high-throughput function determination. Science, 2000, 289(5485):1760~1763 [19] Davies H, Lomas L, Austen B. Profiling of amyloid β peptide variants using SELDI Protein Chip arrays. BioTechniques, 1999, 27(6):1258~1261 [20] Koopmann J, Zhang Z, White N, et al. Serum diagnosis of pancreatic adenocarcinoma using surface-enhanced laser desorption and ionization mass spectrometry. Clin Cancer Res, 2004, 10(3):860~868 [21] Nelson R W, Nedelkov D, Tubbs K A, et al. Biosensor chip mass spectrometry: a chip-based proteomics approach. Electrophoresis, 2000, 21(6):1155~1163 [22] Zhu H, Bilgin M, Bangham R, et al. Global analysis of protein activities using proteome chips. Science,2001,293 : 2101~2105 [23] Uetz P, Giot L, Cagney G, et al. A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae. Nature, 2000, 403(6770):623~627 [24] Uetz P. Two-hybrid arrays. Curr Opin Chem Biol, 2002, 6 :57~62 (ON01910) Cancer Cell Plk1 ON01910 I