PROTEOMICS TODAY Protein Assessment and Biomarkers Using Mass Spectrometry, 2D Electrophoresis, and Microarray Technology
|
|
|
- Vernon Horn
- 5 years ago
- Views:
Transcription
1 PROTEOMICS TODAY Protein Assessment and Biomarkers Using Mass Spectrometry, 2D Electrophoresis, and Microarray Technology MAHMOUD HAMDAN GlaxoSmithKline PIER GIORGIO RIGHETTI University of Verona, Italy A JOHN WILEY & SONS, INC., PUBLICATION
2
3 PROTEOMICS TODAY
4
5 PROTEOMICS TODAY Protein Assessment and Biomarkers Using Mass Spectrometry, 2D Electrophoresis, and Microarray Technology MAHMOUD HAMDAN GlaxoSmithKline PIER GIORGIO RIGHETTI University of Verona, Italy A JOHN WILEY & SONS, INC., PUBLICATION
6 Copyright C 2005 by John Wiley & Sons, Inc. All rights reserved. Published by John Wiley & Sons, Inc., Hoboken, New Jersey. Published simultaneously in Canada. No part of this publication may be reproduced, stored in a retrieval system, or transmitted in any form or by any means, electronic, mechanical, photocopying, recording, scanning, or otherwise, except as permitted under Section 107 or 108 of the 1976 United States Copyright Act, without either the prior written permission of the Publisher, or authorization through payment of the appropriate per-copy fee to the Copyright Clearance Center, Inc., 222 Rosewood Drive Danvers, MA 01923, , fax , or on the web at Requests to the Publisher for permission should be addressed to the Permissions Department, John Wiley & Sons, Inc., 111 River Street, Hoboken, NJ 07030, (201) , fax (201) Limit of Liability/Disclaimer of Warranty: While the publisher and author have used their best efforts in preparing this book, they make no representations or warranties with respect to the accuracy or completeness of the contents of this book and specifically disclaim any implied warranties of merchantability or fitness for a particular purpose. No warranty may be created or extended by sales representatives or written sales materials. The advice and strategies contained herein may not be suitable for your situation. You should consult with a professional where appropriate. Neither the publisher nor author shall be liable for any loss of profit or any other commercial damages, including but not limited to special, incidental, consequential, or other damages. For general information on our other products and services please contact our Customer Care Department within the U.S. at , outside the U.S. at or fax Wiley also publishes its books in a variety of electronic formats. Some content that appears in print, however, may not be available in electronic format. Library of Congress Cataloging-in-Publication Data Proteomics today: Protein assessment and biomarkers using mass spectrometry, 2D electrophoresis, and microarray technology / Mahmoud Hamdan, Pier Giorgio Righetti. p. cm. Includes bibliographical references and index. ISBN (cloth) 1. Proteins Spectra. 2. Mass spectrometry. 3. Proteomics. I. Hamdan, Mahmoud, 1947-II. Righetti, P. G. QP551.M dc Printed in the United States of America
7 To Fatima, Giovanna, Mohammed, Jamilah To my nephews Mohsin, Hamdan, Mahmoud and Suad M.H.
8
9 CONTENTS PREFACE TO PART I ACKNOWLEDGMENT XV XVII I INTRODUCTION TO PART I 1 1 INSTRUMENTATION AND DEVELOPMENTS Introduction / Ionization Techniques for Macromolecules / Cf Plasma Desorption Ionization / Fast Atom/Ion Bombardment / Type of Fragment Ions and Nomenclature / Examples on Analytical Solutions Based on FAB MS / Detection of Abnormalities in Hemoglobin / Glycoprotein Structure Determination / Early Scanning Functions on Sector Machines / Electrospray Ionization / Matrix-Assisted Laser Desorption Ionization / MALDI at High Pressure / 21 vii
10 viii CONTENTS Desorption Ionization on Silicon (DIOS) / Delayed Extraction / Ion Detection / Microchannel Plates (MCPs) / Cryogenic Detectors / Superconducting Tunnel Junction / Thermal Detectors / Types of Analyzers / Quadrupole Mass Filter / Three-Dimensional Quadrupole Ion Trap / Linear Ion Trap / Time of Flight / Fourier Transform Ion Cyclotron Resonance / Hybrid Analyzers / Quadrupole Time of Flight / Ion Mobility TOF / Linear Ion Trap FT ICR / Ion Trap TOF / Tandem Mass Spectrometry / Postsource Decay / MS MS Measurements / Collisional Activation / Current MS Instrumentation in Proteome Analyses / MALDI TOF / MALDI TOF TOF / FT ICR MS / ION Mobility MS / Current MS-Based Proteomics / Delivering Peptides to Ion Source / Peptide Sequencing and Database Searching / Peptide Mass Fingerprinting / Searching with MS MS Data / Databases for MS Data Search / Recent Achievements and Future Challenges / Current Applications / Signal Transduction Pathways / Concluding Remarks / 61 References / 63
11 CONTENTS ix 2 PROTEOMICS IN CANCER RESEARCH Introduction / Two-Dimensional Gel Electrophoresis / Surface-Enhanced Laser Desorption Ionization / Protein Microarrays / Getting More Than Just Simple Change in Protein Expression / Laser Capture Microdissection / Pancreatic Ductal Adenocarcinoma / Analyses Based on Chip Technology / SELDI Analysis of Pancreatic Ductal Adenocarcinoma / Protein Profiling Following Treatment with DNA Methylation/Histone Deacetylation Inhibitors / Proteomic Profiling of PDAC Following Treatment with Trichostatin A / Proteomic Profiling of PDAC Following Treatment with 5 -aza-2 -deoxycytidine / Proteomic Analysis of Human Breast Carcinoma / Two-DE Analysis in Breast Cancer / Proteomic Profiling of Breast Cancer Cell Membranes / Proteomic Analysis on Selected Tissue Samples / Proteomic Profiling of Chemoresistant Cancer Cells / Protein Alterations in Pancreas Carcinoma Cells Exposed to Anticancer Drug / Proteomic Profiling of Cervix Squamous Cell Carcinoma Treated with Cisplatin / Signal Pathway Profiling of Prostate Cancer / Emerging Role of Functional and Activity-Based Proteomics in Disease Understanding / Activity-Based Protein Profiling / Probing Protein Functions Using Chromophore-Assisted Laser Inactivation / Role of Protein Tyrosine Kinases / Concluding Remarks and Future Prospects / 109 References / CURRENT STRATEGIES FOR PROTEIN QUANTIFICATION Introduction / Strategies Based on Labeling a Specific Amino Acid Residue / Isotope-Coded Affinity Tags / 128
12 x CONTENTS Two-Dimensional Gel Electrophoresis/MALDI TOF MS / Labeling Lysine Residues / Labeling Tryptophan Residues / Methionine-Containing Peptides / Global Internal Standard Technology / Acylation / Esterification / Incorporation of Heavy Isotopes / Differential In-Gel Electrophoresis / Quantification of Modified Proteins / Detection and Quantification of Phosphorylated Proteins / Detection and Quantification of Glycosylated Proteins / Ubiquitination / Comments and Considerations / Method of Separation / Detection / Other Approaches / Imaging Mass Spectrometry / Microarrays in Protein Quantification / Protein Recognition Molecules / Surface Chemistry / Some Applications / Emerging Role of Microfluidic Devices / Sample Preparation and Sample Delivery / Concluding Remarks / 174 References / 177 II PROTEOMICS TODAY: SEPARATION SCIENCE AT WORK CONVENTIONAL ISOELECTRIC FOCUSING IN GEL MATRICES AND CAPILLARIES AND IMMOBILIZED ph GRADIENTS Introduction / Brief Historical Survey / Conventional Isoelectric Focusing in Amphoteric Buffers / General Considerations / Basic Method / 190
13 CONTENTS xi Applications and Limitations / Specific Advantages / Carrier Ampholytes / Equipment / Electrophoretic Equipment / Polymerization Cassette / Polyacrylamide Gel Matrix / Reagents / Gel Formulations / Choice of Carrier Ampholytes / Gel Preparation and Electrophoresis / Assembling Gel Mold / Filling Mold / Gel Polymerization / Sample Loading and Electrophoresis / General Protein Staining / Micellar Coomassie Blue G-250 / Coomassie Blue R-250/CuSO 4 / Coomassie Blue R-250/Sulfosalicylic Acid / Coomassie Blue G-250/Urea/Perchloric Acid / Silver Stain / Specific Protein Detection Methods / Quantitation of Focused Bands / Troubleshooting / Waviness of Bands Near Anode / Burning along Cathodic Strip / ph Gradients Different from Expected / Sample Precipitation at Application Point / Some Typical Applications of IEF / Examples of Some Fractionations / Artifacts or Not? / Immobilized ph Gradients / General Considerations / Problems of Conventional IEF / Immobiline Matrix / Narrow and Ultranarrow ph Gradients / Extended ph Gradients: General Rules for Their Generation and Optimization / 230
14 xii CONTENTS Nonlinear, Extended ph Gradients / Extremely Alkaline ph Gradients / IPG Methodology / Casting an Immobiline Gel / Reswelling Dry Immobiline Gels / Electrophoresis / Staining and ph Measurements / Storage of Immobiline Chemicals / Mixed-Bed CA IPG Gels / Troubleshooting / Some Analytical Results with IPGs / Capillary Isoelectric Focusing / General Considerations / cief Methodology / Increasing Resolution by Altering Slope of ph Gradient / On Problem of Protein Solubility at Their pi / Assessment of ph Gradients and pi Values in cief / Separation of Peptides and Proteins by CZE in Isoelectric Buffers / General Properties of Amphoteric, Isoelectric Buffers / Troubleshooting for CZE in Isoelectric Buffers / Novel EOF Modulators / Conclusions / 260 References / SODIUM DODECYL SULFATE POLYACRYLAMIDE GEL ELECTROPHORESIS Introduction / SDS Protein Complexes: a Refinement of the Model / Theoretical Background of M r Measurement by SDS PAGE / Methodology / Purity and Detection of SDS / Molecular Mass Markers / Prelabeling with Dyes or Fluorescent Markers / Postelectrophoretic Detection / Nondiamine, Silver Nitrate Stain / Colloidal Staining / Hot Coomassie Staining / 288
15 CONTENTS xiii Turbidimetric Protein Detection (Negative Stain) / Negative Metal Stains / Fluorescent Detection / Possible Sources of Artifactual Protein Modification / On Use and Properties of Surfactants / The Use of Surfactants Other Than SDS / Acid-Labile Surfactants / Anomalous Behavior / Gel Casting and Buffer Systems / Sample Pretreatment / Standard Method Using Continuous Buffers / Composition of Gels and Buffers / Use of Discontinuous Buffers / Method of Neville / Method of Laemmli / Porosity Gradient Gels / Peptide Mapping by SDS PAGE / SDS PAGE in Photopolymerized Gels / Blue Native PAGE and Other Native PAGE Protocols / Blotting Procedures / Capillary and Electrophoretic Transfer / Detection Systems after Blotting / Conclusions / 331 References / TWO-DIMENSIONAL MAPS Introduction / Early Days and Evolution of 2D PAGE / A Glimpse at Modern Times / Some Basic Methodology Pertaining to 2D PAGE / Methods of Cell Disruption / Proteolytic Attack during Cell Disruption / Precipitation Procedures / Removal of Interfering Substances / Solubilization Cocktail / Sample Application / Sequential Sample Extraction / True Artifacts and Fata Morganas / 371
INTERNATIONAL PROJECT MANAGEMENT:
INTERNATIONAL PROJECT MANAGEMENT: LEADERSHIP IN COMPLEX ENVIRONMENTS International Project Management: Leadership in Complex Environments Copyright 2010 John Wiley & Sons, Inc. ISBN: 978-0-470-57882-7
t e g y s t r a i m p l e m e n t a t i o n M E N TA L L A N G U A G M O D E L S S T O R Y M A P S S O C I A L M E D I A
D E S I G N V I S U A L T H I N K I N G L A N G U A G V I R T U A L W O R K S O C I A L M E D I A s t r a M E E T t e g y V I S U A L I N G S R T U P S C O L L A B O R AT I O N S T O R Y M A P S M O D
The Procurement and Supply Manager s Desk Reference
The Procurement and Supply Manager s Desk Reference The Procurement and Supply Manager s Desk Reference Second Edition FRED SOLLISH, MS JOHN SEMANIK, MBA John Wiley & Sons, Inc. Copyright 2012 by John
Troubleshooting the Sequencing Batch Reactor
Troubleshooting the Sequencing Batch Reactor Illustrations by Brittany Lytle A John Wiley & Sons, Inc., Publication Troubleshooting the Sequencing Batch Reactor WASTEWATER MICROBIOLOGY SERIES Editor Nitrification
Commonsense Talent Management
Commonsense Talent Management Commonsense Talent Management USING STRATEGIC HUMAN RESOURCES TO IMPROVE COMPANY PERFORMANCE Steven T. Hunt Copyright 2014 by John Wiley & Sons, Inc. All rights reserved.
Managing Exports Navigating the Complex Rules, Controls, Barriers, and Laws Frank Reynolds
Managing Exports Navigating the Complex Rules, Controls, Barriers, and Laws Frank Reynolds JOHN WILEY & SONS, INC. Managing Exports Managing Exports Navigating the Complex Rules, Controls, Barriers,
LIFE CYCLE RELIABILITY ENGINEERING
LIFE CYCLE RELIABILITY ENGINEERING Life Cycle Reliability Engineering. Guangbin Yang Copyright 2007 John Wiley &Sons, Inc. ISBN: 978-0-471-71529-0 LIFE CYCLE RELIABILITY ENGINEERING Guangbin Yang Ford
STRATEGIC MARKETING FOR HEALTH CARE ORGANIZATIONS
STRATEGIC MARKETING FOR HEALTH CARE ORGANIZATIONS Building a Customer-Driven Health System PHILIP KOTLER, JOEL SHALOWITZ, AND ROBERT J. STEVENS STRATEGIC MARKETING FOR HEALTH CARE ORGANIZATIONS STRATEGIC
STRUCTURAL STABILITY OF STEEL: CONCEPTS AND APPLICATIONS FOR STRUCTURAL ENGINEERS
STRUCTURAL STABILITY OF STEEL: CONCEPTS AND APPLICATIONS FOR STRUCTURAL ENGINEERS Structural Stability of Steel: Concepts and Applications for Structural Engineers Theodore V. Galambos Andrea E. Surovek
Diagnosing and Changing Organizational Culture
Diagnosing and Changing Organizational Culture To download PDFs of the OCAI (Organizational Culture Assessment Instrument) and the MSAI (Management Skills Assessment Instrument) please go to www.josseybass.com/go/cameron.
Forensic Accounting and Fraud Investigation for Non-Experts
Forensic Accounting and Fraud Investigation for Non-Experts Forensic Accounting and Fraud Investigation for Non-Experts Third Edition HOWARD SILVERSTONE MICHAEL SHEETZ STEPHEN PEDNEAULT FRANK RUDEWICZ
Sarbanes-Oxley Internal Controls
Sarbanes-Oxley Internal Controls Effective Auditing with AS5, CobiT, and ITIL ROBERT R. MOELLER John Wiley & Sons, Inc. Sarbanes-Oxley Internal Controls Sarbanes-Oxley Internal Controls Effective Auditing
OPERATIONAL REVIEW WORKBOOK Case Studies, Forms, and Exercises Rob Reider John Wiley & Sons, Inc. New York Chichester Weinheim Brisbane Toronto Singap
OPERATIONAL REVIEW WORKBOOK Case Studies, Forms, and Exercises Rob Reider John Wiley & Sons, Inc. New York Chichester Weinheim Brisbane Toronto Singapore OPERATIONAL REVIEW WORKBOOK OPERATIONAL REVIEW
Proteomics. Proteomics is the study of all proteins within organism. Challenges
Proteomics Proteomics is the study of all proteins within organism. Challenges 1. The proteome is larger than the genome due to alternative splicing and protein modification. As we have said before we
MANAGEMENT OF TECHNOLOGY
MANAGEMENT OF TECHNOLOGY MANAGEMENT OF TECHNOLOGY Managing Effectively in Technology-Intensive Organizations Hans J. Thamhain This book is printed on acid-free paper. Copyright 2005 by John Wiley & Sons,
BEST PRACTICES IN PLANNING AND PERFORMANCE MANAGEMENT RADICALLY RETHINKING MANAGEMENT FOR A VOLATILE WORLD. Third Edition. David A. J.
BEST PRACTICES IN PLANNING AND PERFORMANCE MANAGEMENT RADICALLY RETHINKING MANAGEMENT FOR A VOLATILE WORLD Third Edition David A. J. Axson Best Practices in Planning and Performance Management Best Practices
Scrivener Publishing 100 Cummings Center, Suite 541J Beverly, MA
Biofuels Production Scrivener Publishing 100 Cummings Center, Suite 541J Beverly, MA 01915-6106 Publishers at Scrivener Martin Scrivener (martin@scrivenerpublishing.com) Phillip Carmical (pcarmical@scrivenerpublishing.com)
Executive Compensation Best Practices
Executive Compensation Best Practices Frederick D. Lipman Steven E. Hall John Wiley & Sons, Inc. Executive Compensation Best Practices Executive Compensation Best Practices Frederick D. Lipman Steven
Sarbanes-Oxley and the New Internal Auditing Rules
Sarbanes-Oxley and the New Internal Auditing Rules ROBERT R. MOELLER John Wiley & Sons, Inc. Sarbanes-Oxley and the New Internal Auditing Rules Sarbanes-Oxley and the New Internal Auditing Rules ROBERT
How to Estimate with. RSMeans Data
How to Estimate with RSMeans Data How to Estimate with RSMeans Data Basic Skills for Building Construction Fourth Edition RSMeans and Saleh A. Mubarak, Ph.D. Barbara Balboni, Technical Editor RSMeans
Bridge Design and Evaluation
Bridge Design and Evaluation Bridge Design and Evaluation LRFD and LRFR Gongkang Fu John Wiley & Sons, Inc. Cover Design: Michael Rutkowski Cover Photograph: Courtesy of Alamy Creativity/Alamy This book
COUPLED BIOLUMINESCENT ASSAYS
COUPLED BIOLUMINESCENT ASSAYS Methods, Evaluations, and Applications MICHAEL J. COREY A John Wiley & Sons, Inc., Publication COUPLED BIOLUMINESCENT ASSAYS COUPLED BIOLUMINESCENT ASSAYS Methods, Evaluations,
MANAGEMENT ACCOUNTING BEST PRACTICES
MANAGEMENT ACCOUNTING BEST PRACTICES A Guide for the Professional Accountant STEVEN M. BRAGG John Wiley & Sons, Inc. Management Accounting Best Practices A Guide for the Professional Accountant MANAGEMENT
Credit Risk Scorecards
Credit Risk Scorecards Developing and Implementing Intelligent Credit Scoring Naeem Siddiqi John Wiley & Sons, Inc. Additional praise for Credit Risk Scorecards... An essential book for anyone interested
Sarbanes-Oxley. Guide for Finance and Information Technology Professionals SANJAY ANAND. John Wiley & Sons, Inc.
Sarbanes-Oxley Guide for Finance and Information Technology Professionals SANJAY ANAND John Wiley & Sons, Inc. Sarbanes-Oxley Guide for Finance and Information Technology Professionals Sarbanes-Oxley
SOFTWARE EVOLUTION AND MAINTENANCE
SOFTWARE EVOLUTION AND MAINTENANCE A PRACTITIONER S APPROACH PRIYADARSHI TRIPATHY KSHIRASAGAR NAIK SOFTWARE EVOLUTION AND MAINTENANCE SOFTWARE EVOLUTION AND MAINTENANCE A Practitioner s Approach PRIYADARSHI
Business Intelligence Competency Centers A Team Approach to Maximizing Competitive Advantage
Business Intelligence Competency Centers A Team Approach to Maximizing Competitive Advantage Gloria J. Miller Dagmar Bräutigam Stefanie V. Gerlach John Wiley & Sons, Inc. Business Intelligence Competency
Proteomics And Cancer Biomarker Discovery. Dr. Zahid Khan Institute of chemical Sciences (ICS) University of Peshawar. Overview. Cancer.
Proteomics And Cancer Biomarker Discovery Dr. Zahid Khan Institute of chemical Sciences (ICS) University of Peshawar Overview Proteomics Cancer Aims Tools Data Base search Challenges Summary 1 Overview
Accounts Payable Best Practices Mary S. Schaeffer Executive Editor IOMA s Report on Managing Accounts Payable Co-creator The Accounts Payable Certification Programs John Wiley & Sons, Inc. Accounts Payable
WELDING METALLURGY SECOND EDITION Sindo Kou Professor and Chair Department of Materials Science and Engineering University of Wisconsin A JOHN WILEY & SONS, INC., PUBLICATION WELDING METALLURGY SECOND
Protein Methods. Second Edition. DANIEL M. BOLLAG Merck Research Laboratories West Point, Pennsylvania
Protein Methods Second Edition DANIEL M. BOLLAG Merck Research Laboratories West Point, Pennsylvania MICHAEL D. ROZYCKI Department of Chemistry The Henry H. Hoyt Laboratory Princeton University Princeton,
For more information on any of the above titles, please visit
ESSENTIALS of XBRL Financial Reporting in the 21st Century Bryan Bergeron John Wiley & Sons, Inc. ESSENTIALS of XBRL Essentials Series The Essentials Series was created for busy business advisory and
The Executive Director s GUIDE
JOSSEY-BASS NONPROFIT GUIDEBOOK S E R I E S The Executive Director s GUIDE TO THRIVING AS A NONPROFIT LEADER SECOND EDITION SECOND EDITION MIM CARLSON MARGARET DONOHOE The Executive Director s Guide to
CORPORATE FINANCE WORKBOOK
CORPORATE FINANCE WORKBOOK CFA Institute is the premier association for investment professionals around the world, with over 101,000 members in 134 countries. Since 1963 the organization has developed
Civil Engineer s Handbook of Professional Practice
Civil Engineer s Handbook of Professional Practice Civil Engineer s Handbook of Professional Practice Karen Lee Hansen and Kent E. Zenobia John Wiley & Sons, Inc. This book is printed on acid-free paper.
Proteomics and some of its Mass Spectrometric Applications
Proteomics and some of its Mass Spectrometric Applications What? Large scale screening of proteins, their expression, modifications and interactions by using high-throughput approaches 2 1 Why? The number
JOHN BASCHAB JON PIOT
T H E PROFESSIONAL SERVICES FIRM BIBLE JOHN BASCHAB JON PIOT John Wiley & Sons, Inc. T H E PROFESSIONAL SERVICES FIRM BIBLE T H E PROFESSIONAL SERVICES FIRM BIBLE JOHN BASCHAB JON PIOT John Wiley & Sons,
2-Dimensional Electrophoresis
2-Dimensional Electrophoresis Proteomics Pathway Proteomics Pathway History of 2-DE 1956 -paper & starch gel 1975 -Coupling of IEF & SDS-PAGE IPG-IEF 2DE-Techniques Immobilized ph Gradient IsoElectric
INTEGRATION OF ALTERNATIVE SOURCES OF ENERGY
INTEGRATION OF ALTERNATIVE SOURCES OF ENERGY FELIX A. FARRET M. GODOY SIMÕES A JOHN WILEY & SONS, INC., PUBLICATION INTEGRATION OF ALTERNATIVE SOURCES OF ENERGY INTEGRATION OF ALTERNATIVE SOURCES OF
LECTURE-3. Protein Chemistry to proteomics HANDOUT. Proteins are the most dynamic and versatile macromolecules in a living cell, which
LECTURE-3 Protein Chemistry to proteomics HANDOUT PREAMBLE Proteins are the most dynamic and versatile macromolecules in a living cell, which regulates essential activities of the cell. The classical protein
About This Book. Why is this topic important? What can you achieve with this book? How is this book organized?
About This Book Why is this topic important? Trainers sometimes fail to ask enough questions or the right question prior to designing, redesigning, or presenting a training program as the solution to a
DESIGN OF WATER SUPPLY PIPE NETWORKS
DESIGN OF WATER SUPPLY PIPE NETWORKS DESIGN OF WATER SUPPLY PIPE NETWORKS Prabhata K. Swamee Ashok K. Sharma Copyright # 2008 by John Wiley & Sons, Inc. All rights reserved Published by John Wiley & Sons,
GETTING STARTED IN PERSONAL AND EXECUTIVE COACHING
GETTING STARTED IN PERSONAL AND EXECUTIVE COACHING How to Create a Thriving Coaching Practice Stephen G. Fairley Chris E. Stout John Wiley & Sons, Inc. GETTING STARTED IN PERSONAL AND EXECUTIVE COACHING
Lecture 5: 8/31. CHAPTER 5 Techniques in Protein Biochemistry
Lecture 5: 8/31 CHAPTER 5 Techniques in Protein Biochemistry Chapter 5 Outline The proteome is the entire set of proteins expressed and modified by a cell under a particular set of biochemical conditions.
Frequently Asked Questions
K O U Z E S P O S N E R THE LEADERSHIP CHALLE NGE VALUES CARDS Frequently Asked Questions JAMES M. KOUZES AND BARRY Z. POSNER, WITH JO BELL AND RENEE HARNESS i Copyright 2010 by James M. Kouzes & Barry
John Wiley & Sons, Inc.
Corporate Fraud Case Studies in Detection and Prevention JOHN D. O GARA John Wiley & Sons, Inc. Corporate Fraud Corporate Fraud Case Studies in Detection and Prevention JOHN D. O GARA John Wiley & Sons,
marketing 3.0 PHILIP KOTLER From Products to Customers to the Human Spirit JOHN WILEY & SONS, INC.
marketing 3.0 marketing 3.0 From Products to Customers to the Human Spirit PHILIP KOTLER HERMAWAN KARTAJAYA IWAN SETIAWAN JOHN WILEY & SONS, INC. Copyright C by 2010 by Philip Kotler, Hermawan Kartajaya,
A Guide to Creating a Successful Algorithmic Trading Strategy
A Guide to Creating a Successful Algorithmic Trading Strategy The Wiley Trading series features books by traders who have survived the market s ever changing temperament and have prospered some by reinventing
EDGAR H. SCHEIN A N D R E V I S E D E D I T I O N N E W
EDGAR H. SCHEIN THE CORPORATE CULTURE SURVIVAL GUIDE A N D R E V I S E D E D I T I O N N E W The Corporate Culture Survival Guide Join Us at Josseybass.com Register at www.josseybass.com/email for more
COMPLYING WITH. SECTION 404 A Guide for Small Publicly Held Companies SARBANES- OXLEY LYNFORD GRAHAM
COMPLYING WITH SARBANES- OXLEY SECTION 404 A Guide for Small Publicly Held Companies LYNFORD GRAHAM Complying with Sarbanes-Oxley Section 404 Complying with Sarbanes-Oxley Section 404 A Guide for Small
ECONOMICS FOR INVESTMENT DECISION MAKERS WORKBOOK
ECONOMICS FOR INVESTMENT DECISION MAKERS WORKBOOK CFA Institute is the premier association for investment professionals around the world, with over 117,000 members in 134 countries. Since 1963 the organization
Strategies in proteomics
Strategies in proteomics Systems biology - understand cellpathways, network, and complex interacting (includes Genomics, Proteomics, Metabolomics..) Biological processes - characterize protein complexes,
Bioinformatics (Lec 19) Picture Copyright: the National Museum of Health
3/29/05 1 Picture Copyright: AccessExcellence @ the National Museum of Health PCR 3/29/05 2 Schematic outline of a typical PCR cycle Target DNA Primers dntps DNA polymerase 3/29/05 3 Gel Electrophoresis
Proteomics and Vaccine Potency Testing
Proteomics and Vaccine Potency Testing Louisa B. Tabatabai, PhD Respiratory Diseases of Livestock Research Unit National Animal Disease Center, ARS, USDA, Ames, Iowa What is proteomics Methodologies of
Zakopane, April, 2011
Clinical Proteomics: A Technology to shape the future? Zakopane, April, 2011 Protein Chemistry/Proteomics/Peptide Synthesis and Array Unit Institute of Biomedicine/Anatomy Biomedicum Helsinki, 00014 University
Alternative to 2D gel electrophoresis OFFGEL electrophoresis combined with high-sensitivity on-chip protein detection.
Alternative to 2D gel electrophoresis OFFGEL electrophoresis combined with high-sensitivity on-chip protein detection Application Note Christian Wenz Andreas Rüfer Abstract Agilent Equipment Agilent 3100
STAGING TO SELL THE SECRET TO SELLING HOMES IN A DOWN MARKET BARB SCHWARZ SOLD SOLD SOLD THE INVENTOR OF HOME STAGING
STAGING TO SELL THE SECRET TO SELLING HOMES IN A DOWN MARKET SOLD SOLD SOLD BARB SCHWARZ THE INVENTOR OF HOME STAGING STAGING TO SELL STAGING TO SELL The Secret to Selling Homes in a Down Market BARB
The. Rational. Project Manager A THINKING TEAM S GUIDE TO GETTING WORK DONE. Andrew Longman Jim Mullins KEPNER-TREGOE, INC. John Wiley & Sons, Inc.
The Rational Project Manager A THINKING TEAM S GUIDE TO GETTING WORK DONE Andrew Longman Jim Mullins KEPNER-TREGOE, INC. John Wiley & Sons, Inc. The Rational Project Manager The Rational Project Manager
Supplementary Material. The MIAPE-GE glossary (MIAPE-GE version 1.4).
Supplementary Material. The MIAPE-GE glossary (MIAPE-GE version 1.4). Classification Definition 1. General features 1.1.1 Date stamp The date on which the work described was initiated; given in the standard
Proteomics and Cancer
Proteomics and Cancer Japan Society for the Promotion of Science (JSPS) Science Dialogue Program at Niitsu Senior High School Niitsu, Niigata September 4th 2006 Vladimir Valera, M.D, PhD JSPS Postdoctoral
MANAGING AND LEADING SOFTWARE PROJECTS RICHARD E. (DICK) FAIRLEY A JOHN WILEY & SONS, INC., PUBLICATION
MANAGING AND LEADING SOFTWARE PROJECTS RICHARD E. (DICK) FAIRLEY A JOHN WILEY & SONS, INC., PUBLICATION MANAGING AND LEADING SOFTWARE PROJECTS Press Operating Committee Chair Linda Shafer former Director,
WELDING METALLURGY AND WELDABILITY OF NICKEL-BASE ALLOYS
WELDING METALLURGY AND WELDABILITY OF NICKEL-BASE ALLOYS John N. DuPont John C. Lippold Samuel D. Kiser A JOHN WILEY & SONS, INC., PUBLICATION WELDING METALLURGY AND WELDABILITY OF NICKEL-BASE ALLOYS
Protein microarray and proteomics
17 2 2005 4 Chinese Bulletin of Life Sciences Vol. 17, No. 2 Apr., 2005 1004-0374(2005)02-0132-05 510515 ; Q503;Q51 A Protein microarray and proteomics FEI Jia, MA Wen-Li*, ZHENG Wen-Ling (Institute of
ENTERPRISE PERFORMANCE MANAGEMENT DONE RIGHT
ENTERPRISE PERFORMANCE MANAGEMENT DONE RIGHT ENTERPRISE PERFORMANCE MANAGEMENT DONE RIGHT AN OPERATING SYSTEM FOR YOUR ORGANIZATION Ron Dimon Copyright 2013 by John Wiley & Sons, Inc. All rights reserved.
Laboratory Water Quality Affects Protein Separation by 2D Gel Electrophoresis
Laboratory Water Quality Affects Protein Separation by 2D Gel Electrophoresis 2D gel electrophoresis remains a dominant technique in proteomics. Obtaining high quality gels requires careful and tedious
12/6/12. Dr. Sanjeeva Srivastava IIT Bombay
Dr. Sanjeeva Srivastava IIT Bombay Gel-based proteomics 2-DE work-flow 1 Isoelectric focusing (first dimension) 2 Equilibration of IPG strips 3 SDS-PAGE (second dimension) 4 Staining gel visualization
Determination of Isoelectric Point (pi) By Whole-Column Detection cief
Determination of Isoelectric Point (pi) By Whole-Column Detection cief Tiemin Huang and Jiaqi Wu CONVERGENT BIOSCIENCE Determination of Isoelectric Point (pi) by Whole Column Detection cief Definition
How to Be an Investment Banker
How to Be an Investment Banker Founded in 1807, John Wiley & Sons is the oldest independent publishing company in the United States. With offices in North America, Europe, Australia and Asia, Wiley is
Protein Electrophoresis EZ-Run Protein Gel Solution EZ-Run Protein Standards EZ-Run Gel Staining Solution Traditional SDS-PAGE Reagents
Protein Electrophoresis EZ-Run Protein Gel Solution EZ-Run Protein Standards EZ-Run Gel Staining Solution Traditional SDS-PAGE Reagents Reliability. Purity. Certainty. Introduction Sodium dodecyl sulfate
392 Index. peptide N-glycosidase F. treatment, 331
Index 391 Index A Albumins, seed protein extraction, 20, 21, 23 Antibody screening, see Protein microarray Arabidopsis thaliana, see Chloroplast; Plasma membrane B Blue-native polyacrylamide gel (BN-PAGE),
DATA MINING AND BUSINESS ANALYTICS WITH R
DATA MINING AND BUSINESS ANALYTICS WITH R DATA MINING AND BUSINESS ANALYTICS WITH R Johannes Ledolter Department of Management Sciences Tippie College of Business University of Iowa Iowa City, Iowa Copyright
JOHN WILEY & SONS, INC.
Consultation Skills for Mental Health Professionals Richard W. Sears John R. Rudisill Carrie Mason-Sears JOHN WILEY & SONS, INC. Consultation Skills for Mental Health Professionals Consultation Skills
ELECTROPHORESIS TECHNOLOGY: GLOBAL MARKETS. MST040C April Andrew McWilliams Project Analyst ISBN:
ELECTROPHORESIS TECHNOLOGY: GLOBAL MARKETS MST040C April 2013 Andrew McWilliams Project Analyst ISBN: 1-56965-303-8 BCC Research 49 Walnut Park, Building 2 Wellesley, MA 021 866-285-7215, 781-9-7301 www.bccresearch.com
Advances in analytical biochemistry and systems biology: Proteomics
Advances in analytical biochemistry and systems biology: Proteomics Brett Boghigian Department of Chemical & Biological Engineering Tufts University July 29, 2005 Proteomics The basics History Current
Governance, Risk Management, and Compliance
Additional praise for Governance, Risk Management, and Compliance It Can t Happen to Us Avoiding Corporate Disaster While Driving Success In this complex and perilous global marketplace, it is vital that
SUPPLY CHAIN MANAGEMENT. Best Practices. Second Edition. David Blanchard
SUPPLY CHAIN MANAGEMENT Best Practices Second Edition David Blanchard Supply Chain Management Best Practices Supply Chain Management Best Practices Second Edition DAVID BLANCHARD John Wiley & Sons, Inc.
Leadership for the Common Good
Leadership for the Common Good Tackling Public Problems in a Shared-Power World Second Edition Barbara C. Crosby John M. Bryson Leadership for the Common Good Leadership for the Common Good Tackling
Streetsmart Financial Basics for Nonprofit Managers
Streetsmart Financial Basics for Nonprofit Managers Third Edition Thomas A. McLaughlin John Wiley & Sons, Inc. Streetsmart Financial Basics for Nonprofit Managers Streetsmart Financial Basics for Nonprofit
Enterprise Risk Management
Enterprise Risk Management A Methodology for Achieving Strategic Objectives Gregory Monahan John Wiley & Sons, Inc. Enterprise Risk Management Enterprise Risk Management A Methodology for Achieving Strategic
Computing with large data sets
Computing with large data sets Richard Bonneau, spring 2009 Lecture 14 (week 8): genomics 1 Central dogma Gene expression DNA RNA Protein v22.0480: computing with data, Richard Bonneau Lecture 14 places
EFFECTIVE INTERPERSONAL AND TEAM COMMUNICATION SKILLS FOR ENGINEERS
EFFECTIVE INTERPERSONAL AND TEAM COMMUNICATION SKILLS FOR ENGINEERS IEEE Press 445 Hoes Lane Piscataway, NJ 08854 IEEE Press Editorial Board 2012 John Anderson, Editor in Chief Ramesh Abhari George W.
Chapter 3. Exploring Proteins and Proteomes. Dr. Jaroslava Miksovska
Chapter 3 Exploring Proteins and Proteomes Dr. Jaroslava Miksovska Chapter objectives: Protein purification and isolation techniques Protein sequencing Immunology for protein identification Protein identification
FUNDAMENTALS OF SEMICONDUCTOR MANUFACTURING AND PROCESS CONTROL
FUNDAMENTALS OF SEMICONDUCTOR MANUFACTURING AND PROCESS CONTROL FUNDAMENTALS OF SEMICONDUCTOR MANUFACTURING AND PROCESS CONTROL Gary S. May, Ph.D. Georgia Institute of Technology Atlanta, Georgia Costas
Organizational Consulting How to Be an Effective Internal Change Agent
Organizational Consulting How to Be an Effective Internal Change Agent Alan Weiss, PhD John Wiley & Sons, Inc. Organizational Consulting Also by Alan Weiss THE ULTIMATE CONSULTANT SERIES Life Balance:
Chapter 6. Techniques of Protein and Nucleic Acid Purification
Chapter 6 Techniques of Protein and Nucleic Acid Purification Considerations in protein expression and purification Protein source Natural sources Recombinant sources Methods of lysis and solubilization
1-DE 2-DE??? PCPPAU 04/2011
1-DE 2-DE??? PCPPAU 04/2011 THEORY Any charged ion or group of ions will migrate when placed in an electric field. ph -----IEP (pi) Migration is dependent on charge density Charge/Mass + - PCPPAU 04/2011
Molecular characterization, detection & quantitation of biological products Purin Charoensuksai, PhD
Molecular characterization, detection & quantitation of biological products Purin Charoensuksai, PhD Department of Biopharmacy, Faculty of Pharmacy, Silpakorn University Example of critical checkpoints
Kinetics Review. Tonight at 7 PM Phys 204 We will do two problems on the board (additional ones than in the problem sets)
Quiz 1 Kinetics Review Tonight at 7 PM Phys 204 We will do two problems on the board (additional ones than in the problem sets) I will post the problems with solutions on Toolkit for those that can t make
Separation of Native Monoclonal Antibodies and Identification of Charge Variants:
Separation of Native Monoclonal Antibodies and Identification of Charge Variants: Teamwork of the Agilent 31 OFFGEL Fractionator, Agilent 21 Bioanalyzer and Agilent LC/MS Systems Application Note Biosimilar
Analysis of Protein Biopharmaceuticals
Analysis of Protein Biopharmaceuticals Comprehensive cgmp Services at Every Stage of Drug Development Amazing where you can go At Solvias, we work closely with you Solvias provides comprehensive services
Instant-Bands Protein Sample Loading Buffer for SDS-PAGE. User s Manual. View Protein Bands in an SDS Gel. Instantly. EZBiolab.
Instant-Bands Protein Sample Loading Buffer for SDS-PAGE User s Manual View Protein Bands in an SDS Gel Instantly www.ezbiolab.com 2 Instant-Bands User s Manual Table of Contents Introduction 3 Storage
Fraud and Fraud Detection
Fraud and Fraud Detection The Wiley Corporate F&A series provides information, tools, and insights to corporate professionals responsible for issues affecting the profitability of their company, from accounting
Faster, easier, flexible proteomics solutions
Agilent HPLC-Chip LC/MS Faster, easier, flexible proteomics solutions Our measure is your success. products applications soft ware services Phospho- Anaysis Intact Glycan & Glycoprotein Agilent s HPLC-Chip
Advanced Protein Electrophoresis. Advanced Protein Electrophoresis. Learning Objective. Proteomics ; Molecular & cell Biology
Electrophoresis is a powerful technique for finer protein separation and visualization of separated proteins. It is based on the principle of migration of charged proteins in an electric field. Electrophoretic
Project Management. Budgeting, Tracking, and Repor ting. Costs and Prof itability second edition
Praise for Project Management Accounting Project Management in relation to a company s mission, objectives, and strategy Project Management in an agile business Coverage of agile Project Management as
Enterprise Risk Management
Enterprise Risk Management Join Us at Josseybass.como Register at www.josseybass.com/email for more information on our publications, authors, and to receive special offers. Enterprise Risk Management A
Managing the Unexpected
Managing the Unexpected Managing the Unexpected Sustained Performance in a Complex World Third Edition Karl E. Weick Kathleen M. Sutcliffe Cover image: istock.com / ImageGap Cover design: Wiley Copyright
THROUGHPUT ACCOUNTING
THROUGHPUT ACCOUNTING A GUIDE TO CONSTRAINT MANAGEMENT STEVEN M. BRAGG JOHN WILEY &SONS, INC. THROUGHPUT ACCOUNTING A GUIDE TO CONSTRAINT MANAGEMENT THROUGHPUT ACCOUNTING A GUIDE TO CONSTRAINT MANAGEMENT
DRUG SAFETY EVALUATION
DRUG SAFETY EVALUATION DRUG SAFETY EVALUATION SHAYNE C. GAD A John Wiley & Sons, Inc., Publication This book is printed on acid-free paper. Copyright # 2002 by John Wiley and Sons, Inc., New York. All
Microchip Electrophoresis for Glycoprotein Separation. Analytical Strategy, September 30th, 2014 Karina Hasler & Adrian Müller
Microchip Electrophoresis for Glycoprotein Separation Analytical Strategy, September 30th, 2014 Karina Hasler & Adrian Müller Karina Hasler & Adrian Müller 30.09.2014 1 Content Glycoproteins Available