2014 BIO World Congress on Industrial Biotechnology and Bioprocessing Philadelphia, Pennsylvania May 13 th, 2014
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1 2014 BIO World Congress on Industrial Biotechnology and Bioprocessing Philadelphia, Pennsylvania May 13 th, 2014
2 Phytonix s biosynthesis technology does not convert biomass into fuels and chemicals The biomass converts carbon dioxide directly into the target fuels and chemicals.. A Generation 4 biofuels technology
3 Using synthetic biology, photobiology and genomics technologies, we have Developed transformant cyanobacterial organisms as fuel production platforms. N-butanol is currently being produced directly from carbon dioxide feedstock. - This should result in low cost and high-yielding biofuels. - Removing the biomass from the biofuels cost equation can eliminate up to 75% of the production cost.
4 $8bn n-butanol + $900bn motor gasoline markets $1.35 per gallon projected production cost IP portfolio & carbon-negative production process World-class scientific development team Multiple exit options - M&A, Sub-license, JV, IPO Seeks syndicate investors for $6m Series A round n-butanol
5 In 2010, Px purchased the exclusive global license to globally patentpending genomics and synthetic biology technologies to develop environmentally safe, high yielding, direct solar liquid transportation fuels. In January, 2014, Phytonix received a USPTO Notice of Allowance on its core IP. Follow-on IP is patent-pending.
6 International IP Portfolio INVENTOR U.S.P.T.O. Notice of Allowance, U.S.12/918,784, IP globally patent-pending Designer organisms for photobiological butanol production from carbon dioxide and water Own exclusive licensing option on patent-pending/patent-granted follow-on butanol/pentanol IP AUGUST 2010: EXCLUSIVE GLOBAL LICENSE WITH FULL SUB-LICENSING RIGHTS PHYTONIX Phytonix technology is patent-pending in many countries. Sub-Licensing opportunities. Inventor is Dr. James Lee, who is on faculty at Old Dominion University (Norfolk, VA)
7 US$ Billions 1, x increase Global Motor Gasoline Market China car purchases Higher crude oil prices Biofuels adoption Wholesale price. Sources: IAE, EAI, Clean West Capital estimates
8 GLOBAL BUTANOL MARKET (US$bn) BUTANOL PRICE (US$000 per metric ton) CAGR 12% n-butanol Propylene Source: Nexant
9 (Bio)butanol (n-butanol) is a four-carbon, straight-chain alcohol. Used to run spark ignition engines (e.g., gasolinepowered vehicles) Can replace gasoline since the energy content of the two fuels is nearly the same. Global industrial chemical market for n-butanol is $ 9 billion with a 12+% CAGR
10 Quality Methanol Ethanol Butanol Gasoline Chemistry CH 3 OH C 2 H 5 OH C 4 H 10 OH Many Energy Content 63,000 Btu 78,000 Btu 110,000 Btu 115,000 Btu (per gallon) Vapor Pressure 4.6 PSI 2.0 PSI 0.33 PSI F (Reid V.P.) Motor Octane Air to Fuel Ratio Higher energy content, less evaporative (safer), no seasonal blends required, can be used as a drop-in gasoline replacement fuel, large n-butanol industrial chemical market
11 Production generates lbs. CO2
12
13 Source: Purves et al., 2003
14 Calvin Cycle 2- Phosphoglycerate Pyruvate Fructose-6- phosphate 1,3- Diphosphoglycerate Butyraldehyde 20,22 12,44 Glycogen 17,18,19 Fructose- 1,6- diphosphate 1- Butanol
15 Enzyme Source Organism GenBank #/Protein ID NADH-dependent Butanol dehydrogenase (12) Glucose-phosphate isomerase (18) Fructose-diphosphate aldolase (20) Clostridium carboxidivorans Saccharomyces cerivisiae Chlamydomonas reinhardtii ADC48983 M21696 JGI Chlre3 protein ID
16 Modular PhytoconverterTM Systems: Designed for direct biobutanol and biopentanol fuel/chemical production with static cyanobacterial cultures and back-end separation system. Modular, continuous batch process Will allow Px production facilities to scale from small industrial operations to large-scale commercial facilities
17 Separation technology Will use <energy than other approaches in use now Will achieve >8% n-butanol concentration At which point it will "phase separate" into 100% n-butanol Will occur through state-of-the-art membrane filtration technology and pervaporation
18 Net 16 lbs CO2 reduced per gallon of n-butanol produced Our butanol & pentanol fuel lifecycles from prodution through combustion are carbonneutral Butanol and pentanol combust more cleanly than gasoline and other fossil-based fuels: Complete combustion of n-butanol emits only CO2 and water No emissions of carbon monoxide, hydrocarbons or SOx
19 Cost Effective: Px s technology will be far more cost-effective than biomass and petroleum-based fuels: < $2.00/gallon High Yields: ~15,000-30,000 gallons of n- butanol or n-pentanol per acre/year.
20 Phytonix s proprietary biosafety-guarded technology can be used for other transgenic and modified organisms and applications Phytonix owns the exclusive rights to sublicense this technology and will do so at a reasonable cost to encourage its use.
21 Each biofuel generation uses less land to produce a given volume of biofuel Algal/CB photobioreactor based biofuels have by far the highest productivity yield per acre.
22 We expect to develop highly adaptive organisms that proliferate in a variety of environments and media Direct sunlight, ambient sunlight, cool to warm temperatures, and fresh, salt, and brackish water This adaptability will enhance the global adoption of Px s photobiological fuel production technology.
23 & Answers
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