Electro Water Separation : a Breakthrough in Harvesting and Microbial Control Facilitates Commercialization of Algae Biomass Systems

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Electro Water Separation : a Breakthrough in Harvesting and Microbial Control Facilitates Commercialization of Algae Biomass Systems By Jose

1 Electro Water Separation : a Breakthrough in Harvesting and Microbial Control Facilitates Commercialization of Algae Biomass Systems By Jose Sanchez Piña, VP QA & Services, OriginOil Inc Pacific Rim Summit, San Diego CA, December 9th, OriginOil, Inc. Pacific Rim Summit 2013

2 Important Disclaimer Matters discussed in this presentation contain forward-looking statements within the meaning of the Private Securities Litigation Reform Act of When used in this presentation, the words "anticipate," "believe," "estimate," "may," "intend," "expect," poised, and similar expressions identify such forward-looking statements. Actual results, performance or achievements could differ materially from those contemplated, expressed or implied by the forward-looking statements contained herein. These forward-looking statements are based largely on our expectations and are subject to a number of risks and uncertainties. These include, but are not limited to, risks and uncertainties associated with our history of losses and our need to raise additional financing, the acceptance of our products and technology in the marketplace, our ability to demonstrate the commercial viability of our products and technology and our need to increase the size of our organization. Further information on our risk factors is contained in our quarterly and annual reports as filed with the Securities and Exchange Commission. As a result there can be no assurance that the forward-looking statements included in this presentation will prove to be accurate or correct. In light of these risks, uncertainties and assumptions, the future performance or events described in the forward-looking statements in this presentation might not occur. Accordingly, you should not rely upon forward-looking statements as a prediction of actual results and we do not assume any responsibility for the accuracy or completeness of any of these forward-looking statements. We undertake no obligation to revise or update publicly any forward-looking statements for any reason.

3 OriginOil: In a few words... OriginOil develops & licenses breakthrough technologies that solve major problems in expanding multi-billion-dollar energy industries Proprietary technologies boost yields, slash cost, and return profits in: Algae harvesting and shelf life Ammonia elimination and disinfection of aquaculture water 98% decontamination of oil & gas frack water Independent tests and trials in US government and commercial labs, Pacific Rim and European partner sites verify breakthrough results Technology protected by 29 patents pending; Australia grants the first Income streams from scale-up and deployment partners Creating and receiving enthusiastic media coverage Loyal following of large and small stockholders Proven management team with bull's-eye industry experience

4 From R&D to Commercialization THE ALGAE MARKET TAKES OFF 4

5 ALGAE MARKETS Large Biofuel Market (High Volume Low Value) In 2010, algae biofuels markets were $217 million By 2015, they are forecast to reach $1.6 billion Mounting global pressure for renewables (France s Green Buildings law) Cost and profits are the challenge 2013: Algae fuel producers receive $1.01/gallon tax credit on output Specialty Markets (Lower Volume Much Higher Value) Opportunities for green chemicals and other high-value end products Emerging markets for green commodities: chemicals, feed, fertilizer High value products such as: Omega 3, astaxanthin, pharmaceuticals, plastics What Is Our Industry's Greatest Challenge? Extracting algae from the water up to 1000:1 water to algae! Crashes in growth ponds from bacteria, rotifers, ciliates and adverse algae Shelf life once harvested Source: Algae Biofuels Production Technologies Worldwide Market Research Report

6 The Solutions to the Challenges OriginOil's Electro Water Separation Greatest Challenge Solved by OriginOil's EWS Technology Extracting algae from the water up to 1000:1 water to algae! OriginOil s EWS Algae for harvesting Crashes in growth ponds from bacteria, rotifers, ciliates & adverse algae OriginOil s EWS Algae Screen for enhanced growth Shelf life once harvested OriginOil s EWS Algae for harvesting 2012 OriginOil, Inc.

The OriginOil EWS Algae Harvesting Solution OriginOil s breakthrough algae harvesting system: Lower capital and operating costs than any other de-watering

7 The OriginOil EWS Algae Harvesting Solution OriginOil s breakthrough algae harvesting system: Lower capital and operating costs than any other de-watering process High speed Energy efficient Chemical free Completely scalable Integrates upstream and down Now a standardized, selling product line: The EWS Algae

The EWS Algae A12 for R&D Fully integrated algae harvester Dewaters more thoroughly Decontaminates to extend shelf life Model 12 delivers up to 12 LPM In commercial production and sales Entry-level,

8 The EWS Algae A12 for R&D Fully integrated algae harvester Dewaters more thoroughly Decontaminates to extend shelf life Model 12 delivers up to 12 LPM In commercial production and sales Entry-level, low-cost Testing, R&D, process improvement Will process 33% of daily harvest at 60,000-liter/day facility Options: Decontamination, pre-harvest stimulation, capacity upgrade Operator training, literature and support included

9 The EWS Algae A200 Commercial algae harvester rated at 200 liters/minute Larger harvesters also available

contamination, temperature, grown in autotrophic or

10 Versatile, Adaptable, Rugged Operates with all algae types and conditions: Any strain, salinity, degree of contamination, temperature, grown in autotrophic or heterotrophic mode Harvesting algae grown in light Harvesting algae grown in dark

11 Savings Over Other Methods

12 Savings Over Other Methods

13 Savings Over Other Methods

14 Two-Stage Harvesting System 1. Single-Step Extraction (SSE) neutralizes algae cells electrical charge so algae clump together (flocculate). This is a "flocculation" type SSE, which does not damage the algae. 2. Hydrogen Flotation creates a gas cloud of micro-bubbles pushing algae solids upwards for surface collection (Video of Appliance Model 12)

15 Stage One: Efficient Dewatering OriginOil s first stage dewaters algae more efficiently than any other solution Single Step Extraction eliminates fatal flaws of current dewatering methods: ORIGINOIL EWS Algae Removes Bacteria 2013 OriginOil, Inc. Pacific Rim Summit 2013

Stage Two: Integrated Concentration Hydrogen Flotation integrates closely with the extraction stage to concentrate the algae into a highdensity

16 Stage Two: Integrated Concentration Hydrogen Flotation integrates closely with the extraction stage to concentrate the algae into a highdensity slurry No further equipment is required to achieve 5 to 10% concentration of solids Surface concentrate and clear effluent are fully ready for next steps

17 Additional Advantages Clean Growth: OriginOil s Algae Screen works on living algae to eliminate most bacteria, rotifers, ciliates and adverse algae Decontaminated algae grows better, demonstrating improved yield at harvest* Degree of improvement exceeds 50%, with potentials exceeding 80% Extended Shelf Life: Normally, algae begins to rot after harvesting; short shelf-life complicates yield/roi EWS Algae decontamination delivers shelf-life up to one month** * Source: OriginOil Announces Breakthrough Innovation to Increase Algae Yield ** Source: OriginOil Internal Estimate (3 rd party study in progress)

18 EWS Aqua Removes Bacteria (with no Algae harm) 1 2 Note: these results depend on conductivity, electrode alloy and flow rate 3 P. aeruginosa after several exposure steps (pay attention at cell walls) 2013 OriginOil, Inc. Pacific Rim Summit 2013

Additional Advantages Promise to Change the Paradigms in Asthaxantin Production: EWS Algae can Harvest HP green cells

and remove bacteria from HP We have empirically seen that this concentrated, viable, cleansed and stressed paste of

We have more cells, given that we do not have attacks from predators/bacteria in the green phase; preliminary results

19 Additional Advantages Promise to Change the Paradigms in Asthaxantin Production: EWS Algae can Harvest HP green cells yielding viable cells for shift EWS Algae can Harvest HP red Cysts and concentrate them EWS Algae EM pulses stress and remove bacteria from HP We have empirically seen that this concentrated, viable, cleansed and stressed paste of HP green cells once placed inside a stressing reactor induces a more controlled metamorphosis into the red cysts. We have more cells, given that we do not have attacks from predators/bacteria in the green phase; preliminary results show that the cells present faster rates of evolution into cysts. We can harvest the red cysts as well to dry them swiftly

Sounds Familiar? Lab Conditions Algae Screen Model 20 The scale up of Haematococcus in green phase often stops due to crashing as soon as the culture leaves the lab.

20 Sounds Familiar? Lab Conditions Algae Screen Model 20 The scale up of Haematococcus in green phase often stops due to crashing as soon as the culture leaves the lab. The use of Algae Screen as it passes to the next level reduces the possibility of crashing, allowing to reach the thousands of liters level required for commercial production. To inoculate large systems CRASH!! 2013 OriginOil, Inc. Pacific Rim Summit 2013

21 Additional Advantages Haematococcus pluvialis sustains production with Algae Screen Average Doubling time 2 days Average Doubling time 2 Days Average Doubling time 4 days Average Doubling time 4 days (Low Voltage, Low Amperage) (High Voltage, Low Amperage) (Low Voltage, High Amperage) (High Voltage, High Amperage) The normal doubling time for this strain and growth conditions without Algae Screen is 6.9 days (if it does not crash due to adverse microorganisms) 2013 OriginOil, Inc. Pacific Rim Summit 2013

22 What can OriginOil do for Algae Farmers? The Usual Suspects that Crash Algae in Ponds and Reactors Parasitic Diatom Bacteria Rotifer Fungus Ciliate YOUR STRAIN Adverse Algae

23 What can OriginOil do for Algae Farmers? The Drama of Contamination (Conventional Paradigms) "It's contaminated, it needs to be discarded" Use of Antibiotics Use of Chemicals such as Sodium Hypochlorite or Hydrogen Peroxide Changes in Salinity, ph or nutrients Several days to recover production levels due to Algae Stress/damage

24 What can OriginOil do for Algae Farmers? When Contaminated Harvest the problems away 10,000 Liters of Contaminated Algae Culture at 300 mg/l 50 Liters of Clean Algae Paste at mg/l 90 to 99% Less Bacteria 9950 Liters of Clean Water at 15 mg/l Harvest with Appliance

25 What can OriginOil do for Algae Farmers? When Contaminated Harvest the problems away Algae Paste (The cells are totally alive) Water Sterilized with OOIL SSE, Chlorination, UV, Hydrogen Peroxide, etc Back to Production in a few Hour$$$!!!

small photobioreactors Need Clean Culture

15,000 gallon levels Inoculation of algal

26 Algae Farmers Basic Need for Microbiological Control Need Clean Inoculum? From a few cells to Inoculation Carboys in the Culture Room Need Clean Inoculum? Culture intermediate scale up to 300 gallons in small photobioreactors Need Clean Inoculum? Culture production in large photobioreactors up to 15,000 gallon levels Inoculation of algal culture and nutrients in the production bags DO YOU REALLY THINK WE NEED CLEAN INOCULUM?? Production bags or ponds Harvest Biomass without Bacteria Increased Shelf Life/Quality

27 What can OriginOil do for Algae Farmers? Clean Intake Water and Inoculum Supply Bleach? 1 ml/l and Survives UV Lamps? 70% survival OriginOil EWS Aqua Terminator

28 What can OriginOil do for Algae Farmers? Increase in Production through Heterotrophic Jump Terminator class SSE 2013 OriginOil, Inc. Pacific Rim Summit 2013

transforms it into nitrates in seconds (so we can clean fish water and get fertilizer for algae).

29 What can OriginOil do for Algae Farmers? Sell your Algae to Aquaculture producers and get fast revenue The first stage of the OriginOil Appliance system (SSE), equipped with an special alloy core, can remove Ammonia from water and transforms it into nitrates in seconds (so we can clean fish water and get fertilizer for algae). This would decrease capital costs of Aquaculture (more animals per pond, and use the land of ammonia-reduction ponds for production) OriginOil Appliance provides algae meal which is cheaper and more nutritious than conventional corn, soybean and fish meal-based balanced food OriginOil SSE can kill bacteria, virus and parasites that attack fish/shrimp species and induce crashes in production (and for algae, this will provide you a clean water source) OriginOil, Inc. Pacific Rim Summit 2013

30 What can OriginOil do for Algae Farmers? Sell your Algae to Aquaculture producers and get fast revenue The lack of cheap aquafeed is constraining the development of aquaculture Source: Dr. Ignace Debruyne & Associates Consultancy

31 What can OriginOil do for Algae Farmers? Sell your Algae to Aquaculture producers and get fast revenue The current price of fishmeal is around $1700 US per metric ton. Aquaculture farmers would buy your algae if you could provide more competitive prices. The use of the appliance allow production costs below $800 US per ton for good and clean algae biomass to substitute fishmeal. Fishmeal monthly price US $/Metric Ton Source:

32 Aquafeed will pay your bills now, is a step to future your goals OriginOil technologies help farmers to provide the best-cleanest algae meal possible First Stage ( ) Second Stage ( ) Third Stage (2017 and up) Products Partners Markets/Customers Spirulina Fishmeal Omega 3 Algasol OriginOil GNC Aquaculture Farms Nutraceutics Phycocyanin Phycoerytrin Asthaxantin Algasol OriginOil BioMolecular Nutraceutics Garden State BioE L Oreal Farmacias Similares (Mex) Genoma Labs European Pharmaceuticals Bio-jetfuel JP5 Biodiesel BioGasoline Algasol OriginOil IH2 Shell, Exxon, BP Rolls Royce or GE US NAVY, Siemens, GE, Airlines, Automobile fuel markets Initial Investment 4 Million Dlls 25 Million Dlls Million Dlls Anual Sales 5 Million Dlls 100 Million Dlls Million Dlls 32

33 "Automobile will never replace horses" (We are in good company) Late XIX British Self Propelled vehicle RED flag act, required - 1. Self-propelled vehicles to have a speed limit of 4 mph(6 Km/h) in country roads and 2 mph (3 Km/h) in city roads 2. It should have a crew of 3 a driver, a stoker and flag man 3. The flag man need to carry a Red flag and walk 60 yards (55 m) ahead of the vehicle. This effectively restricted the speed of the vehicle to the walking speed of the man carrying the Red flag. He has to warn the horse carriages about the self-propelled vehicle ahead and ensure that the driver stops the vehicle till the horse or the horse carriage passes by. The amended Highway & Locomotive act of 1878 reduced the distance of the Red flag man to 20 yards but all the other conditions remained same. These restrictive rules and regulations choked the development of the British Motor Industry, helped by the Railway and Horse carriage lobbies

34 "Automobiles will never replace horses" (We are in good company) (Proposal by Camden County Freeholders to Ban Automobiles in 1900) Henry Ford in his late XIX Century Quadracycle

35 "Automobiles will never replace horses" (We are in good company) As an Algae Producer Why do you keep using horses to control your microbiological management issues. If you have access to a Model T that will change the game from now on?

36 If you want to see these technologies Thermal CA test site for Algae and Aqua EWS December 18 th, 2013 CA 86 Hwy and 81 St, Thermal CA, 11AM to 2 PM

37 Thanks!! Jose L Sanchez Piña VP QA & Services OriginOil Inc. jose@originoil.com jlsanchez@post.harvard.edu +1 (714) OriginOil, Inc. Pacific Rim Summit 2013

Use of Algae Appliance for Harvesting and Microbiological Control of Algal Biomass

Use of Algae Appliance for Harvesting and Microbiological Control of Algal Biomass By Jose Sanchez Piña, General Manager Algae Division, OriginOil Inc European Algae Biomass, Vienna Austria, April 24-25,