NUTRIENT REDUCTION THROUGH THE USE OF ADVANCED BIOLOGICAL NUTRIENT RECOVERY. Rick Johnson Vice President, Market Development

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1 NUTRIENT REDUCTION THROUGH THE USE OF ADVANCED BIOLOGICAL NUTRIENT RECOVERY Rick Johnson Vice President, Market Development 1

2 Agenda What is the trend Technology description Results Economics "Vision without action is a daydream. Action without vision is a nightmare -Japanese Proverb 2

3 State Nitrogen and Phosphorus Criteria Level 5 Level 4 Level 3 Level 2 Level 1 Complete set of N and P criteria for all watertypes 2 or more watertypes with N and/or P criteria 1 watertype with N and/or P criteria Some waters with N and/or P criteria No N and/or P criteria Source: 3

4 2016 (est)

5 Percentage Numeric Criteria Trend: States & Territories Level 1 Level 2 Level 3 Level 4 Level 5 100% 80% 60% 40% 20% 0% est Level Level Level Level Level

6 ????

7 The challenge: Reducing targeted nutrients in a cost effective manner Our solution: View algae as a process not a product Sustainable, advanced nutrient recovery o US Patent 8,101,080 The benefits: Best in Class performance No chemical sludge produced Sustainable Multi-generational, single technology CO 2 recycling & O 2 release Biomass co-product 7

8 CO2 MIX Algae Return NUTRIENT RECOVERY SEPARATION Pure oxygen Clean Water Algae Harvest 8

9 TP, mg/l Controlled experiment: Nitrogen : Phosphorus relationship Raw Permeate Target NH3 dosing Date 9

10 Concentration, mg/l Multi constituent performance Municipal Summary Raw Treated TP TN NH3 TKN BOD TSS Raw Treated Nutrient Recovery Test 10

11 Result, mg/l Membranes alone cannot remove dissolved phosphorus Influence of separation on phosphorus reduction Run1 TP Ortho Run Raw Separation Permeate Raw Separation Permeate 11

12 Dissolved Oxygen, mg/l Dissolved Oxygen Median of 1 minute recordings from 2.5 month component evaluation year DMR average (ECHO) 5.2 Current DMR ABNR 12

13 5 MGD Municipality Primarily residential 36,000 Residents TMDL: 0.075mg/L TP Municipal wastewater treatment Constituent Before After % Removal Total P (mg/l) % TSS (mg/l) % PCB (pg) % 13

14 9/20/2010 9/21/2010 9/22/2010 9/23/2010 9/24/2010 9/25/2010 9/26/2010 9/27/2010 9/28/2010 9/29/2010 9/30/ /1/ /2/ /3/ /4/ /5/ /6/ /7/ /8/ /9/ /10/ /11/ /12/ /13/ /14/ /15/2010 Total Phosphorus, mg/l CPF EFF Flow, mgd Second 21 day pilot: Average Raw TP: 0.151mg/L Average Permeate TP: 0.024mg/L 1.4 Post Falls Effluent AlgEvolve Effluent PF Flow, mgd M Ts W Th F Sa Su M Ts W Th F Sa Su M Ts W Th F Sa Su M Ts W Th F 14

15 Results: Constituent Before After % Removal Total P (mg/l) % Ammonia (mg/l) % Total N (mg/l) %

16 3 MGD Pulp & Paper Process recycled paper 130 year old company TMDL: ~0.075mg/L TP Inland Empire Paper Company Results 16

17 Total Phosphorus, mg/l 2-Jun 9-Jun 16-Jun 23-Jun 30-Jun 7-Jul 14-Jul 21-Jul 28-Jul 4-Aug 11-Aug 18-Aug 25-Aug 1-Sep 8-Sep 15-Sep 22-Sep 29-Sep 6-Oct 13-Oct 20-Oct 27-Oct 3-Nov 10-Nov 17-Nov 24-Nov 1-Dec 8-Dec 15-Dec 22-Dec 29-Dec 5-Jan 12-Jan 19-Jan 26-Jan 2-Feb 9-Feb Average Raw TP: 0.126mg/L Average Permeate TP: 0.024mg/L 10 Total Phosphorus Trend Raw Treated Average Raw: 0.126mg/L Average Permeate: 0.024mg/L Separation Technology Vendor performance evaluations

18 45 MGD Municipality 250,000 residents Biosolids management for 14 communities Heavy Industrial Base (26 SIU s) Seasonal Permit: 0.1mg/L TP & 5.0mg/L TN 18

19 Average concentration, mg/l Total Phosphorus Raw Treated Raw Treated

20 TN, mg/l Additional nitrogen reduction Total Nitrogen response Raw Permeate Period 1 Period 2 Period 3 Weekly period 20

21 10/31 11/2 11/5 11/8 11/11 11/14 11/17 11/20 12/9 12/12 12/15 12/18 1/13 1/16 1/19 1/22 1/25 1/28 1/31 2/21 2/24 2/27 3/8 3/11 3/15 3/18 3/21 3/24 3/27 5/14 5/17 5/20 5/23 5/26 6/3 6/6 6/9 6/12 mg/l P Average 3 rd party lab (TP) Feed: 0.285mg/L Permeate: 0.02mg/L Total Phosphorus Feed and Permeate avg Feed Water TP avg Permeate TP 0.1 mg/l P Discharge Permit Feed Alpha Labs Permeate Alpha Labs City Water Baseline Hypo threshold study No light study Permeate results below detection limit of hand held meter-results supplemented with 3 rd party lab results

22 Date COD, mg/l TOC, mg/l Feed Permeate Feed Permeate 3/ / / / / Average Reduction 20.0% 19.4% Numbers in red reported as Non Detect. Level of detection shown 22

23 23

24 10/30/ /6/ /13/ /20/ /27/ /4/ /11/ /18/ /25/2014 1/1/2015 1/8/2015 1/15/2015 1/22/2015 1/29/2015 2/5/2015 2/12/2015 2/19/2015 2/26/2015 Temperature, F Temperature influence Temperature, F Feed PBR Air Average St Dev Feed fluid temp Avg PBR fluid Avg Outsid Air Temp

25 Biomass Market Average Biomass characteristics N, % P2O5, % Potash, % Protein, % Fat, % Carb, % Average Low High Sample size Typical applications 5 Year TAM, $M Bio-plastics $478 - $638 Soil amendment / composting aid $71 - $94 Fuel source $2 - $3 Others In development Average of 25 municipal / industrial sources 25

26 20 year PW Breakeven point for low P reduction at Molar Ratio = 8 Break even technology comparison MBR + chem P ABNR $35,000,000 $30,000,000 $25,000,000 $20,000,000 $15,000,000 $10,000,000 $5,000,000 $ Molar ratio, FeCl3 26

27 Thank You Rick Johnson

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